LOCUS       NZ_CP085300            10216 bp    DNA     linear   CON 18-JAN-2024
DEFINITION  Streptomyces sp. BSE6.1 chromosome, complete genome.
ACCESSION   NZ_CP085300
VERSION     NZ_CP085300.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. BSE6.1
  ORGANISM  Streptomyces sp. BSE6.1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8024389)
  AUTHORS   Ramesh,C., Anwesh,M., Vinithkumar,N.V., Kirubagaran,R. and
            Dufosse,L.
  TITLE     Complete Genome Analysis of Undecylprodigiosin Pigment
            Biosynthesizing Marine Streptomyces Species Displaying Potential
            Bioactive Applications
  JOURNAL   Microorganisms 9 (11), 2249 (2021)
   PUBMED   34835376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8024389)
  AUTHORS   Chatragadda,R. and Maile,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2021) Andaman and Nicobar Centre for Ocean Science
            and Technology, National Institute of Ocean Technology, Dollygunj,
            Port Blair, Andaman and Nicobar Islands 744103, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-OCT-2021
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/18/2024 07:10:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,273
            CDSs (total)                      :: 7,198
            Genes (coding)                    :: 7,094
            CDSs (with protein)               :: 7,094
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 47 of 104
            Pseudo Genes (incomplete)         :: 80 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 30 of 104
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:03:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 475569
            Orig. end                         :: 485785
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085300.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from National Institute of Ocean
            Technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(138..2624)
                     /locus_tag="LJA35_RS02200"
     CDS             complement(138..2624)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978110.1"
                     /locus_tag="LJA35_RS02200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_233644852.1"
                     /transl_table=11
                     /translation="MSHKRCNGDVVEEGTAERGPRALRAEGALKAIAAGPEPRERLRGI
                     LEQALVFAGASFAALYTPGDDPELLCLVVSAGAPRILYGVRDSYPAAGGSPVADAHRAG
                     EAVWIGPEEFAGTAESRHVPSSGFSLAVLPVRGDGGGCLLVLTERPGGFDADDRECLEL
                     IAEAMTCPTPAGAVGTTESGDLQPDAFTLAMDTGRAEVGDRILRLFGLAPEEFDGKVET
                     LLGLTVPEDLPSLMSVTESDHMTIGERELEFRVLQPTGPPKWLRLSGRLLPAGDGRPAR
                     LVGTVADASTLRTDVTDVARVQRLAAALATAGTVRDVSQAVVAALRRPLRADRIALAEL
                     ENDRLVVTVLDPPDPESWPELWRLEWRTEWPDAPVRAMPTLAAALREGRARIWPAGTGD
                     LEPALADVGPGGLAVLPLPAGGRMAGACLIGWDAPHDFGPDERALLTASAGLAGQALMR
                     AHAFDAEHELVGMLQRQLLPRRLPKLPGAVAVARYLPSTAGLELGGDWYDVIPLPDHHV
                     ALVIGDVQGHSAGAATLMGQMRTALRAYAVEGHPPDVVVSHANRLLTGMETDLFATCVY
                     VDLDMEEGSAWCVRAGHLPPVLRHPDGSTEIAEVEGGPPLGVVTQADFPMSPLRLRPGT
                     LIALTTDGLVESPGADIDEGMDRFAHALAVSDPTHLGLVADALLGNARRNDDVALLLVR
                     YDGMALRPLRESWSVWRLPEAVRHARRFTRRTLRSWGVPDDDIDGVLLIVSELVTNALV
                     HTDGKVRLDLTLISGRLRVAVADASPRTPVKPTSIGWEATGGRGILLVEAMSATWGTLP
                     VSGGKQMWSEIPVGGG"
     gene            complement(3123..4397)
                     /locus_tag="LJA35_RS02205"
     CDS             complement(3123..4397)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978109.1"
                     /locus_tag="LJA35_RS02205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S1 family peptidase"
                     /protein_id="WP_233644853.1"
                     /transl_table=11
                     /translation="MSHKRIPKRKAAMAAGGVVALGAAAVLLPNANASQDGGSPDDAAA
                     APRTLKAADASDLASRLAGLLGDTFAGSYYDSGAQQLVVNVAPGDDNQVVMRAKEAGAQ
                     VREVDNSMSELRSGARTLKSEASIPGTSWAIDPRTNKILVTADSTVTGDRWDRLESTVE
                     GLGDGMATVRKSAGTFKTFASGGDAIFGGGARCSLGFNVTAGDGSPAFLTAGHCGVAAA
                     QWSDAQGGRPIATVDQAVFPGQGDFALVTYDDPATEAPSEVNLGDQTLPISGPAEATVG
                     QEVFRMGSTTGLADGQVLGLDATVNYPEGTVTGLIQTDVCAEPGDSGGSLFTRDGLAIG
                     LTSGGSGDCTVGGETFFQPVTTALEAVGATLGDEGAGGGGGGGADGGGGAGDAGAGAGA
                     GESGGAGDAGEGTGVGGDDGSGLTG"
     gene            5001..5216
                     /locus_tag="LJA35_RS02210"
     CDS             5001..5216
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="LJA35_RS02210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_007389398.1"
                     /sec_met_domain="strepbact (E-value: 2.1e-37, bitscore:
                     126.2, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVETVEISDADLDNVSGGLNVNAVGTVTGLVDGIAPVSGL
                     VNTVVGTVEGATGLNTAPVTGLVAGL"
     gene            5316..6122
                     /locus_tag="LJA35_RS02215"
     CDS             5316..6122
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="LJA35_RS02215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_233644854.1"
                     /transl_table=11
                     /translation="MQFRQQALAKLQSPEDLDLPVRFARPQGWLVLSVTLVAMAAASVW
                     AVGGSVTSTVSAPAILTHGQGSYLLQSPVSGQVTAVLARPGQRLAADAPVLKVRTAEGE
                     SVVRTVDAGRVSALAATVGQIISTGANVASVEKVAHADDPLYATVYVPAENAAAIPAHA
                     SVDLTVQSVPTQQYGVLHGEVKSVDRAAQSAQTIGAFLGDGALGEQFTKDGRPVAVTVR
                     LDTSASTKSGYEWSSADGPPFELTSMTLASGSIRLADQRPVDWLLP"
     gene            6119..8341
                     /locus_tag="LJA35_RS02220"
     CDS             6119..8341
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978106.1"
                     /locus_tag="LJA35_RS02220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_233644855.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.2e-39, bitscore:
                     133.9, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTAQETRGRRRAAPPRRSVPKSRAKTVRTPTVLQMEAVECGAAS
                     LAMVLGHYGRHVPLEELRIACGVSRDGSRASNLLKAARSYGFTAKGMQMDLAALAEVTA
                     PAILFWEFNHYVVYDGMGRRFGRRGVYINDPGKGRRFVSLEEFDGSFTGVVLTMEPGDD
                     FTRGGRRPGVLGAMPARLRGTAGTLPAAVLASLLLVAVGAAVPALSRTYIDMFLIGGQT
                     SLLGVLFASMGACVLLTVVLTWLQQANLLHGRIISSTLSSARFLRHLLRLPVTFFSQRS
                     PADLVQRLQSNDAVAETLARDLAAAGVDAIVVVLYAVLLYTYDPQLTFVGIGVALLNIL
                     AMRVVIRLRATRTAKLRADTARLTNTAYTGLQLIETMKATGGEDGYFRKWAGQHATTLE
                     EQQRLGVPSAWLGVVAPTLATLNSALILWIGGMRAVEGHISVGLLVAFQALVVRFTAPL
                     TRLNGVAGRIQDFAADVARLKDVENFRADPLYDRPGGADSTRRLHGHVELQNVSFGYNP
                     LDKPLLTGFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYAPWDGVIRVDGQRLDDIPR
                     GALASSVSFVDQDVFLFEGSVRDNVALWDPSIPDDAVVDALKDAALYDVVMRRPGGIHS
                     PVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAETELVVMDNLRRRGCACV
                     VIAHRLSTVRDSDEIVVLEHGTVVERGRHAELVAHGGAYAALVRER"
ORIGIN
        1 cggacgtact gggtcatggg gtactcactc ctcggttccc gggttccgca cccgctcgtg
       61 gtcgaccggg cgggtacccg tcggggcgcc gcccgcacgc cggtgagggc ggtcccgtcg
      121 ggctcggccc cgggcgctca gccgccgccc accgggatct cgctccacat ctgcttgccc
      181 ccgctgaccg gcagtgtccc ccaggtcgcc gacatcgcct cgaccaggag gatgccccgt
      241 ccgccggtgg cctcccagcc gatactggtc ggcttgaccg gtgtgcgcgg cgaggcgtcg
      301 gcgaccgcga cgcgcagccg gccgctgatc agggtgaggt cgagccgcac cttgccgtcg
      361 gtgtgcacca gggcgttggt gaccagctcg gagacgatca gcagcacgcc gtcgatgtcg
      421 tcgtcgggta cgccccagga gcgcagggtg cgccgggtga agcggcgggc gtgccgtacg
      481 gcctcgggca gccgccacac cgaccagctc tcgcgcagcg ggcgcagggc catgccgtcg
      541 tagcgcacca gcagcagggc gacgtcgtcg ttgcggcggg cgttgcccag cagggcgtcg
      601 gcgaccagtc cgaggtgggt ggggtcggag acggccagcg cgtgcgcgaa ccggtccatg
      661 ccctcgtcga tgtcggcgcc gggcgactcc accaggccgt ccgtggtgag ggcgatgagc
      721 gtgccgggcc gcagccgcag cgggctcatc gggaagtcgg cctgggtgac cacgccgagc
      781 ggcggaccgc cctccacctc ggcgatctcc gtactgccgt cggggtggcg cagcaccggc
      841 ggcagatgtc cggcgcgcac gcaccaggcg gagccctcct ccatgtcgag gtcgacgtag
      901 acgcaggtgg cgaagaggtc ggtctccatg ccggtcagca gccggttggc gtgggagacc
      961 accacgtccg gcgggtggcc ctcgacggcg taggcgcgca gggccgtgcg catctgaccc
     1021 atcagggtgg cggcgccggc gctgtgcccc tggacgtcgc cgatgaccag ggcgacgtgg
     1081 tggtcgggca gcgggatcac gtcgtaccag tcgccgccca gctccagtcc ggccgtgctg
     1141 ggcaggtagc gggcgacggc gaccgcaccg ggcagcttgg gcagccggcg cggcagcaac
     1201 tggcgctgga gcatgccgac cagttcgtgc tcggcgtcga aggcgtgggc gcgcatcagg
     1261 gcctgcccgg cgaggcccgc cgaggcggtg agcagggcgc gctcgtcggg gccgaagtcg
     1321 tgcggggcgt cccagccgat caggcaggcg ccggccatgc ggccgcccgc gggcagcggc
     1381 agtacggcca gaccgccggg gcccacgtcg gcgagtgcgg gctccaggtc accggtgccc
     1441 gccggccaga tccgggcgcg tccctcgcgc agtgcggcgg cgagggtggg catggcccgc
     1501 accggggcgt ccggccactc ggtgcgccac tccaggcgcc acagttcggg ccaggactcg
     1561 gggtcgggcg gatccaggac ggtgacgacg agccggtcgt tctccagctc ggccagcgcg
     1621 atccggtcgg cgcgcagcgg tcggcgcagg gcggcgacca cggcctggct gacgtcgcgc
     1681 acggtcccgg cggtggcgag cgcggcggcg agccgctgga cgcgggcgac atcggtcacg
     1741 tccgtgcgca gggtggaggc gtcggcgacg gtgccgacca gccgggcggg ccggccgtcg
     1801 ccggcgggca gcaggcgtcc gctgagccgg agccacttcg gcgggccggt gggctggagc
     1861 acccggaact ccagctcgcg ctcgccgatg gtcatgtggt cggactcggt caccgacatc
     1921 agcgacggca ggtcctccgg cacggtgagc ccgagcagcg tctccacctt gccgtcgaac
     1981 tcctcggggg cgaggccgaa caggcgcagg atgcggtcgc cgacctccgc ccggccggtg
     2041 tccatggcca gggtgaaggc gtccggctgg aggtcgccgg actcggtggt tccgacggct
     2101 ccggccgggg tggggcaggt catggcctcg gcgatcagtt cgaggcactc gcggtcgtcg
     2161 gcgtcgaacc cgcccgggcg ctcggtcagc acgaggaggc agccgccgcc gtccccgcgc
     2221 acgggcagga ccgccagcga gaagccgctg gacggcacgt gccgcgactc ggccgttccg
     2281 gcgaactcct ccggcccgat ccacaccgcc tcgccggcgc ggtgggcgtc ggcgaccggg
     2341 gagccgccgg ccgcgggata gctgtcccgt acgccgtaca ggatgcgcgg cgcaccggcc
     2401 gacacgacca gacagagcag ctcggggtcg tcgccgggtg tgtagagggc ggcgaaggac
     2461 gcgcccgcga acaccagtgc ctgctcgagg atcccgcgca gccgctcccg gggctccggg
     2521 ccggccgcga tcgccttcag ggcaccctcg gcacgcagcg ccctcggtcc gcgttccgca
     2581 gtgccctctt cgaccacgtc gccattacag cgcttatggg acatctgcgc agcccctacg
     2641 gatcgggtac cggcgcccag gagcacatcg agggctcgcg tacgggcgct tccgccgcag
     2701 gacgggtgcg ggcgcagccc tgccggtggg cacggggcgg acacggccgg tacgcggggc
     2761 gcggggcgga cacgcgccag tgcgcggggc gccggacgga cacgcgccgg tacgcggggg
     2821 gcccgaccgg caccgcgggc gaaacggcga gggccggtgc cgcgggcgaa acggcacgga
     2881 ccggcaccgc gggcgaggcg ggcgcgaccg ggagcggatc ggccgggcag cggggtgggc
     2941 cgggagcccg ggggggcgcg cgccctgccg tccgggcctg cggcgcgccg acgcccgcca
     3001 ccggcggccg cccgccgggt cggtacgtga gccgtacacc cggaccgccg ccccgccggg
     3061 ggtcgggacg gcggcccggt ggacgtgccg tcagccgtca gcccccctcc cccgtcagcc
     3121 cgtcagcccg tcagccccga cccgtcgtcg ccgccgacgc ccgtgccctc acccgcgtca
     3181 ccggcaccac ccgactctcc ggcgccagca cccgcacccg cgtcacctgc acccccaccg
     3241 ccatcggcac ccccaccgcc accgccaccc gcgccttcgt cgccgagggt ggccccgacc
     3301 gcctccaggg cggtggtgac cggctggaag aaggtctcgc cgcccaccgt gcagtcgccg
     3361 ctgccgccgg aggtcagccc gatcgcgagg ccgtcccggg tgaacaggga gccgccgctg
     3421 tcgcccggct cggcgcagac gtcggtctgt atgaggccgg tgacggtgcc ctcggggtag
     3481 ttgacggtgg cgtccaggcc gaggacctgg ccgtcggcca gtccggtcgt gctgcccatg
     3541 cggaagacct cctggccgac cgtcgcctcg gcgggcccgc tgatcggcag ggtctggtcg
     3601 ccgaggttga cctcgctcgg ggcctcggtc gccggatcgt cgtacgtgac gagcgcgaag
     3661 tcgccctggc cggggaagac cgcctggtcg acggtggcga tcggccggcc gccctgcgcg
     3721 tcggaccact gggcggcggc gacgccacag tgaccggccg tcaggaaggc gggcgagccg
     3781 tcgcccgcgg tgacgttgaa gccgagcgag cagcgcgcac cgccgccgaa gatggcgtcc
     3841 ccgcccgacg cgaacgtctt gaaggtgccg gcggacttcc tgaccgtcgc catgccgtcg
     3901 ccgaggccct cgacggtgga ttcgaggcgg tcccaccggt cgccggtgac ggtgctgtcg
     3961 gcggtcacca ggatcttgtt ggtgcgcggg tcgatcgccc acgaggtgcc cgggatgctc
     4021 gcctccgact tgagggtccg tgctccgctc ctgagctcgc tcatgctgtt gtcgacctcg
     4081 cggacctggg cacccgcctc cttcgcccgc atcacgacct ggttgtcgtc gccgggggcg
     4141 acgttgacga cgagctgctg cgcgccggag tcgtagtagg agccggcgaa ggtgtcgccg
     4201 agcagaccgg cgagccgtga ggcgagatcc gacgcgtccg ccgccttgag ggtcctgggc
     4261 gccgcggcgg cgtcgtccgg ggagccgccg tcctgcgagg cgttggcgtt cggcagcagg
     4321 acggcggccg ctccgagtgc gaccacgccg cccgccgcca tcgcggcctt gcgcttggga
     4381 attcgcttgt gactcaacct actcgacctc ctgggaccgg ggttggtacc cgccggtccg
     4441 gcagttgggg gcgcctggac ggctgttggt acgcacgggc cgggcggggt gttcagacgg
     4501 gccgtcgagc cgcgccccgg cgcactccgt ttcacgccga ccctcgacac cctccgcgat
     4561 cacgcgacga cgctctgttc cggccacgga gcggaatgac tgcttcagcg aatctgcaca
     4621 tcgaatgccc aacccggtca cccccgcccg ggcatctgca caagccggcg aggacccgta
     4681 cgcctttggg gccggagtgt cccaatcacc tccgcacggg caagggatcg cacgagccga
     4741 tgtcgcgcac cgtgtgaaga agtcgccgcg aaggcgtgcg cgctcctgca cgcttgggtg
     4801 cccggacgtc gcaagtgcgc tggtgggagg ggtgattgat tggaagcccg gacccgccgc
     4861 gtgctttgat ccatcgcacc gcgggggccg cggagggctc tggaaacggg cgcccggcgc
     4921 ccgtgggccg cggccgtcat ctgtccccag agggggccgc tccccccttg tgaaatggct
     4981 atacgaaggg aagttccatc atgaactcca ccccccaggt tgagaccgtc gagatctcgg
     5041 acgccgacct cgacaacgtc tccggcggcc tgaacgtgaa cgccgtcggc accgtcaccg
     5101 gcctggtgga cggcatcgcc ccggtctccg gcctggtcaa caccgtggtc ggcacggtcg
     5161 agggtgccac cggcctgaac acggccccgg tcacgggcct ggtcgccggt ctctgatcgc
     5221 gcccttgcgt cgctgagtcc cggagccctc caggctccgg gacttttcgg ccgttctccc
     5281 acggccgttc tcgtcgagcc ctggatacgt cagccgtgca gttccgccaa caggccctcg
     5341 caaaactcca gtcaccggag gacctcgatc ttccggtgcg tttcgcccgc ccccagggct
     5401 ggctggtgct gtccgtgacc ctggtcgcga tggccgccgc gtccgtgtgg gcggtgggcg
     5461 gctcggtgac ctccacggtc tccgcgcccg ccatcctcac ccacggacag ggcagttacc
     5521 tcctccagag ccccgtctcc gggcaggtca ccgcggtcct ggcccgaccg ggtcagcggc
     5581 tcgccgccga cgccccggtg ctgaaggtcc gcaccgccga gggcgagagc gtggtccgca
     5641 cggtcgacgc cggccgggtc agcgcgctcg cggccaccgt ggggcagatc atcagtaccg
     5701 gtgccaacgt cgcctcggtg gagaaggtcg cccacgccga cgaccccctc tacgccaccg
     5761 tctacgtccc cgccgagaac gcggccgcca tccccgcgca cgcctccgtc gacctgaccg
     5821 tgcagtcggt gccgacacag cagtacggcg tcctgcacgg cgaggtgaag tccgtggacc
     5881 gcgcggccca gtcggcgcag acgatcggtg ccttcctcgg ggacggcgcg ctcggcgagc
     5941 agttcaccaa ggacggcagg ccggtcgccg tgacggtacg gctggacacc tccgcgtcca
     6001 cgaagagcgg ctacgagtgg tcctcggccg acggaccgcc gttcgaactg acctccatga
     6061 ccctggcctc gggctcgatc cggctggccg accagcgtcc cgtcgattgg ctgctgccgt
     6121 gagcaccgca caggagaccc ggggcagacg ccgcgccgcc ccgccgaggc gcagcgtgcc
     6181 caagagccgg gcgaagaccg tccgtacacc caccgtgctc cagatggagg ccgtggagtg
     6241 cggcgccgcc tccctcgcca tggtgctcgg gcactacggc cgtcatgtcc cgctggagga
     6301 gctgcggatc gcctgcggtg tctcccgcga tgggtcccgc gcgagcaacc tgctgaaggc
     6361 ggcgcgcagt tacggcttca ctgccaaggg catgcagatg gacctggccg cgctcgccga
     6421 ggtgacggca ccggccatcc tcttctggga gttcaaccac tacgtcgtct acgacggcat
     6481 gggccgccgg ttcgggcggc gcggggtgta catcaacgac ccgggcaagg gccgccgttt
     6541 cgtgtccctg gaggagttcg acggctcctt caccggtgtc gtgctgacca tggagcccgg
     6601 tgacgacttc acccgcggcg gccgcaggcc cggtgtgctc ggcgcgatgc cggcccggct
     6661 gcgcggaacc gcgggcacgc tccccgcggc cgtgctggcg agcctgctcc tggtggcggt
     6721 cggcgcggcg gtgcccgcgc tcagccgcac ctacatcgac atgttcctca tcggcgggca
     6781 gacctcgctg ctcggcgtgc tgttcgcgtc gatgggcgcc tgtgtgctgc tcacggtggt
     6841 gctgacctgg ctccagcagg ccaacctgct gcacggccgc atcatctcct ccaccctctc
     6901 cagcgcccgg ttcctgcggc acctgctgcg gctgccggtg accttcttct cccagcgcag
     6961 ccccgccgac ctggtgcagc gcctccagtc caacgacgcg gtcgccgaga cgctggcccg
     7021 cgacctcgcc gcggcgggcg tggacgcgat cgtggtcgtc ctgtacgccg tactgctgta
     7081 cacgtacgac ccgcagctca ccttcgtcgg catcggggtg gcgctgctca acatcctcgc
     7141 gatgcgggtc gtgatccggc tgcgcgcgac ccgtacggcg aagctgcggg cggacacggc
     7201 gcggctgacc aacaccgcgt acaccggcct ccagctgatc gagacgatga aggcgaccgg
     7261 cggcgaggac ggctacttcc gcaagtgggc cggccagcac gccaccacgc tggaggagca
     7321 gcagcggctc ggggtgccga gcgcctggct gggcgtggtc gctccgacgc tcgcgacgct
     7381 gaacagcgcg ctgatcctgt ggatcggcgg tatgcgcgcg gtcgagggcc acatctcggt
     7441 cggtctgctg gtcgccttcc aggcactggt ggtccgcttc acggccccgc tgacccggct
     7501 gaacggcgtc gcgggccgca tccaggactt cgcggccgac gtggcgcggc tgaaggacgt
     7561 ggagaacttc cgggcggacc cgctctacga ccgtcccggc ggcgccgact ccacccgtcg
     7621 gctgcacggt cacgtcgaac tgcagaacgt ctccttcggc tacaacccgc tggacaaacc
     7681 gctgctcacc ggcttcgacc tgaccgtcgg gccggggcag caggtggcgc tggtgggcgg
     7741 ttcgggcagc ggcaagtcga cggtgtcacg gctgatctcg ggcctgtacg cgccctggga
     7801 cggggtgatc cgcgtcgacg ggcagcggct ggacgacatc ccgcgcgggg cgctggcgtc
     7861 ctcggtctcc ttcgtcgacc aggacgtgtt cctgttcgag ggctcggtgc gcgacaacgt
     7921 ggcgctgtgg gacccgtcga tcccggatga cgccgtggtg gacgcactga aggacgcggc
     7981 gctgtacgac gtggtgatgc gcaggccggg cggcatccac agcccggtgg agcaggacgg
     8041 ccggaacttc tccggcgggc agcgccagcg tctggagatc gcccgggcgc tggtgcgccg
     8101 gccgagcatc ctggtcctgg acgaggtgac cagcgccctg gacgcggaga ccgaactggt
     8161 ggtcatggac aacctgcgcc ggcgcggctg cgcctgcgtg gtgatcgcgc accggctcag
     8221 caccgtgcgg gacagcgacg agatcgtcgt cctggagcac ggcacggtcg tggagcgcgg
     8281 gcggcacgcg gagctggtgg cacatggcgg cgcgtacgcg gcgctggtca gggagcggtg
     8341 agatgacgac cgtgcgggaa ggagagggcg acctcgtcct cggggcgctc ggcgcgctgg
     8401 gtacgcgcgt ggactgcgcc gggttcaacc ggctggacct ggaggggccg caggtgctgt
     8461 ggctcgtggt gtccggggcg gtggacctgt tcgcggtgga cgccgcggag cagggccact
     8521 ggcatcacct ggggcgcctg gaggcgggtt ctgtgctgct cgggccggtc gccgggccgc
     8581 agcacacact ggtggcgcgt ccgctgcggg actgcgtcgt gcaccgcatc gcactgcgcg
     8641 agctgtacca gccggcgcac acccagacgt ggtcgtacga cgcgtacggc aatccgcagt
     8701 acgtgccgcc gacgacgagc ccgctggagt acgccctcgc cctcggggtg ggccggggcc
     8761 tcaccgttct cttccaggcg ccgatggccg gcgagcgggc cgacgcgccc acggacgacg
     8821 acgtcttctg gatgcaggtg ccgccgggca gcgtgcagta cggggcggtg tacggcgagg
     8881 aggcggccgc cgacctgctg atggacccgg cgctgtggca gagcatggtc gaccagcagt
     8941 accggctgct gaccaccctg gaccgctgga tcgagcagct ggagcgcacc cacgagacgc
     9001 gtaccgcggc cggtatcaag gccggtgagg cggtgcgcgt ccaggccgac cggacgctgc
     9061 tggcctccat cggcaagcgg tcgggacagc gcaccacggc ggccgacgcc gatgccacgt
     9121 acgcggcctg caggctggtc gcccgcgccg ccggcatccc gctcgccgag ccggcccggg
     9181 gcggcacgga gagcgaccgg ctcgacccgg tggagcgcgt cgcgctcgcg tcgcgggtac
     9241 ggaccaggtc ggtgcggctg gaggaccgct ggtggcggga cgacgtgggg ccgctggtcg
     9301 ggcagcggac cctgtcgggc gcgccggtgg cgctgctgtg gcggcgcggc ggctatgtgg
     9361 ccgtgcatcc ggcgaccggg cgtgagacgc cggtggagaa ggcgaacgcc ggggagttcg
     9421 agccgcgcgc ggtgatgttc taccgtccgc tgcccgagcg ccggctcggt ccgctgcggc
     9481 tgctgcggtt ctgtctggcg ggcacgcgcg gggacctgac gggtctgctg ctcgccggtc
     9541 tggtgacggt ggggctcggc gcgctggtgc cgatcgcgac gggcaaggtg ctcggcgagt
     9601 tcgtgccgaa ggcgcagacc gggctgatcg tgcaggtgtg tctcgcggtg atgctgagca
     9661 gtgtcgtcgc ggcggccttc atgctgttgc agaacctcac catcctgcgc ctggagggcc
     9721 ggatcgaggc gacgctgcaa cccgccgtgt gggaccggct gctgcggctg ccgacgaagt
     9781 tcttcaccga gcgctccacc ggtgagctgg ccagcgcggc catgggcatc agcgcgatcc
     9841 gccggctgct ggcgggggtg ggtccgacgg tggcccagtc ggtgaccgtc ggggcggtga
     9901 acctggtgct gctgctctgg ttcagcgtgc cgatggcgct ggcggcgatc ggcatgctgg
     9961 tggtcgtcgc cggggtgttc ctggggctcg ggctgtggca ggtgcgctgg cagcggcggc
    10021 tggtcaagct caccaacaag ctgaacaacc aggggttcca gacgctgcgc gggctgccca
    10081 agctgcgggt ggcggcggcc gagaactacg cctacgcggc gtgggcggag cggttcgcgc
    10141 acagccgcga gctgcagcgg cgggcggggc ggatcaagaa cctgaacgcg gtgctcggcg
    10201 cggtctacct gccgct
//
