LOCUS       NZ_CP088109            11215 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Sinorhizobium medicae WSM1115 chromosome, complete genome.
ACCESSION   NZ_CP088109
VERSION     NZ_CP088109.1
KEYWORDS    RefSeq.
SOURCE      Sinorhizobium medicae WSM1115 (Ensifer medicae WSM1115)
  ORGANISM  Sinorhizobium medicae WSM1115
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
REFERENCE   1  (bases 1 to 4106266)
  AUTHORS   Kohlmeier,M.G., O'Hara,G.W., Colombi,E., Ramsay,J.P. and
            Terpolilli,J.
  TITLE     Australian commercial rhizobial inoculants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4106266)
  AUTHORS   Kohlmeier,M.G., O'Hara,G.W., Colombi,E., Ramsay,J.P. and
            Terpolilli,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2021) Legume Rhizobium Sciences, Murdoch
            University, 90 South Street, Murdoch, WA 6150, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 149x; 384x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 05:22:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,835
            CDSs (total)                      :: 6,766
            Genes (coding)                    :: 6,456
            CDSs (with protein)               :: 6,456
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 205 of 310
            Pseudo Genes (incomplete)         :: 185 of 310
            Pseudo Genes (internal stop)      :: 32 of 310
            Pseudo Genes (multiple problems)  :: 101 of 310
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:56:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2426223
            Orig. end                         :: 2437438
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP088109.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11215
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11215
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11215
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6215](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6215)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    140..156
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.82"
                     /tool="antismash"
     gene            complement(172..780)
                     /locus_tag="SmedWSM1115_RS12190"
                     /old_locus_tag="SmedWSM1115_12190"
     CDS             complement(172..780)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976334.1"
                     /locus_tag="SmedWSM1115_RS12190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12190"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_011976334.1"
                     /transl_table=11
                     /translation="MAATQNTRERIVSAAARLFYAEGIRAVSVDAVAEAAGLTKRTLYY
                     HFKSKDELIAAYLEERDQPNLKLFQKWFSETDGDAASKIKAIFLNLARAARHPKWKGCG
                     FLRTSAELANMPGHPAIRIAAAHKRKFEDWLRQSFETEGIAEAARLARQMLLLLDGSFA
                     VVLLHRDPSYMETAGEAAATLVKLALSEPSVQQPAGRGK"
     misc_feature    complement(861..881)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 22.49"
                     /tool="antismash"
     gene            864..1655
                     /locus_tag="SmedWSM1115_RS12195"
                     /old_locus_tag="SmedWSM1115_12195"
     CDS             864..1655
                     /NRPS_PKS="Domain: ECH (7-254). E-value: 4.8e-65. Score:
                     211.2. Matches aSDomain:
                     nrpspksdomains_SmedWSM1115_RS12195_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095567.1"
                     /locus_tag="SmedWSM1115_RS12195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12195"
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="WP_011976335.1"
                     /transl_table=11
                     /translation="MSDTVLLKISGGTALLTLNRPERLNALNYELIDRLLTLLDRLEVD
                     AKVHAVILTGAGDRAFSAGGDIHEFSHSVAEGVNAAIRDFVRRGQQLTSRLEAFPKPVI
                     AAVNGIAYGGGCEITEAVHLAIASDRSRFAKPEIKLGMPPTFGGTQRLPRLAGRKRALE
                     LLLTGDAFSAERALEMGLVNAVVPHADLIETARALAARIISHSPVATAAIISAVTRGLN
                     MAIGEGLLCESEQFARMVPTHDLNEGLAAWKERREPRYAGR"
     misc_feature    876..890
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.34"
                     /tool="antismash"
     aSDomain        885..1625
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SmedWSM1115_RS12195_ECH.1"
                     /evalue="4.80E-65"
                     /label="SmedWSM1115_RS12195_ECH.1"
                     /locus_tag="SmedWSM1115_RS12195"
                     /protein_end="254"
                     /protein_start="7"
                     /score="211.2"
                     /tool="antismash"
                     /translation="KISGGTALLTLNRPERLNALNYELIDRLLTLLDRLEVDAKVHAVI
                     LTGAGDRAFSAGGDIHEFSHSVAEGVNAAIRDFVRRGQQLTSRLEAFPKPVIAAVNGIA
                     YGGGCEITEAVHLAIASDRSRFAKPEIKLGMPPTFGGTQRLPRLAGRKRALELLLTGDA
                     FSAERALEMGLVNAVVPHADLIETARALAARIISHSPVATAAIISAVTRGLNMAIGEGL
                     LCESEQFARMVPTHDLNEGLAAWKE"
     gene            complement(1669..2148)
                     /locus_tag="SmedWSM1115_RS12200"
                     /old_locus_tag="SmedWSM1115_12200"
     CDS             complement(1669..2148)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976336.1"
                     /locus_tag="SmedWSM1115_RS12200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12200"
                     /product="anthrone oxygenase family protein"
                     /protein_id="WP_011976336.1"
                     /transl_table=11
                     /translation="MVTLVPVLAFAAVIGSGLMAGLFFVFSVCIMQALSRLPPEQGVAV
                     MNAINVVIQNPLFLIAFMGTALLGLVLLVMAVIWGGQGSYFLAAGGLVYLIGTLAVTIA
                     VNVPLNEALAAAPAGQGATELWQQRYLTEWVRWNHVRSFASTAALALFVLGFART"
     gene            complement(2198..3511)
                     /locus_tag="SmedWSM1115_RS12205"
                     /old_locus_tag="SmedWSM1115_12205"
     CDS             complement(2198..3511)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976337.1"
                     /locus_tag="SmedWSM1115_RS12205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12205"
                     /product="glucoamylase family protein"
                     /protein_id="WP_011976337.1"
                     /transl_table=11
                     /translation="MSLHSRTSDIACIDGLQRAAFEYFLRYSDMRSGLVADTSRAGSPS
                     SIAATGFGLAAYPVAVERGWIARSDAAWRVLTTLRFFAASRQGSDASATGYRGFYYHFL
                     DMRTGDRVWRSELSTIDTSLLLAGALTAAAYFSGRSEEESELRRLAALLYERADWYWAL
                     NGGDTVTMGWRPPGRFLKHRWRGHSEALLLYVLALGSPTRPIMPPNYEAYTAAHEWLEL
                     DGAAHLHAAALFVHLFPQAWIDFRSIRDKGMRDRATDYFDNTRRAIRLQRAHAEENPHG
                     FAGYCRDLWGFSACHAPKGWFRLRDGRRQKLLGYEARGAPFGADDGTLVPWAPLAGLPF
                     EPDACLGSLFHLMSRYPALVKEERLPGGFNPSLPGDGPEGWMDDRLVGLDQGLVVMMIE
                     NWRSGLVWELTRGIPAFRQGLSRAGFAGGWLSSTLLQV"
     gene            3743..4537
                     /locus_tag="SmedWSM1115_RS12210"
                     /old_locus_tag="SmedWSM1115_12210"
     CDS             3743..4537
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013844664.1"
                     /locus_tag="SmedWSM1115_RS12210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12210"
                     /product="DUF1194 domain-containing protein"
                     /protein_id="WP_011976338.1"
                     /transl_table=11
                     /translation="MLGTMALILALSGAPIQSAAISADVDVELLLAVDMSGSMDMEEAR
                     VQRSGYLQALRHPDFINAVQGGLLGRIAIGYFEWAGLVNEASVVSWQVIESSADAEAFA
                     GKLEARPIGTRRGTSISNAILFGSDLIDSNAYSGTRRVIDISGDGPNNTGPPVTPARND
                     ALSRGIIINGLAILIRPSVSTGPLDRYYAECVIGGPGSFVLPVHEPEDFAVAIRQKLIL
                     EVSGREPDPALRLAAGENAADCMIGEKLRPGFLDRVYPELDR"
     gene            complement(4644..4865)
                     /locus_tag="SmedWSM1115_RS12215"
                     /old_locus_tag="SmedWSM1115_12215"
     CDS             complement(4644..4865)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534187.1"
                     /locus_tag="SmedWSM1115_RS12215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12215"
                     /product="hypothetical protein"
                     /protein_id="WP_011976339.1"
                     /transl_table=11
                     /translation="MTNHSSSLTDTEVELASIARMLSHIRESARALEAQAVEYCIDMAL
                     QAVANELRDRGELVIAAAGFDNRTTLLQ"
     gene            complement(5001..6215)
                     /locus_tag="SmedWSM1115_RS12220"
                     /old_locus_tag="SmedWSM1115_12220"
     CDS             complement(5001..6215)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976340.1"
                     /locus_tag="SmedWSM1115_RS12220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12220"
                     /product="YcaO-like family protein"
                     /protein_id="WP_011976340.1"
                     /sec_met_domain="YcaO (E-value: 3.8e-79, bitscore: 264.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIGFAVGSPADTRNAYSDRVLSPEETFDRVRPLLSRFGVTRIARH
                     TGLDRIGLPVWCAYTPNARSIAVAQGKGLTDPEAKVSAVMEALERAVACEPAVGTMVAT
                     ARTLRASGRTVELLPCLIAAGQQDIGPDEELAWALGSDLISGREILVPLAAALLDRTLH
                     SRFWMSSDGLASGNVVEEAILHGLLERIERDAHVLWGISGREHRYGCCVAPESLGDAAL
                     NGLVERIGAAGLDLRLFEITSDIGIPCFLALLGPPDIATRGGGRFVEVTSGCGAHVFPA
                     RAAIRAVTEAAQSRLTFISGARDDVFPETFNRPLPELTRQAFLAEPSRRAIASPQVGGS
                     LEALLRHTVDHLTSAGVQSAIALRLSANALPFSVVKIFVPKLENPDGDRARRFGPRALS
                     KALAS"
     gene            complement(6212..6427)
                     /locus_tag="SmedWSM1115_RS12225"
                     /old_locus_tag="SmedWSM1115_12225"
     CDS             complement(6212..6427)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095572.1"
                     /locus_tag="SmedWSM1115_RS12225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12225"
                     /product="hypothetical protein"
                     /protein_id="WP_024310816.1"
                     /transl_table=11
                     /translation="MAEVVVIGIAGEQGLWLADLKNGTVRPLDQVSGDLATANKLRLQG
                     GTIVKNVDLAVAVSSADKVFSGHFDG"
     gene            complement(6445..8283)
                     /locus_tag="SmedWSM1115_RS12230"
                     /old_locus_tag="SmedWSM1115_12230"
     CDS             complement(6445..8283)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1272: TPR
                     repeat-containing protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095573.1"
                     /locus_tag="SmedWSM1115_RS12230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12230"
                     /product="adenylate cyclase"
                     /protein_id="WP_011976342.1"
                     /transl_table=11
                     /translation="MAVPHFHLQTFGELRLVDSGGVVVACPERGLLILAYLSISGQRTV
                     SREKLSMLIWPEREKSVAFKNLRSTLWRMRHLAANSGPLTSATEIDVTLGEIACDAAGF
                     EALGSSEEAFRAGLSLWRDTFLAGADVPPGRYADWVGEQRSVFTQKLRAALLAGGERFA
                     DRSMLRTACMHLLELDPADTAVRAILERLTGVGLVIGKGSPDGAWLRQGAFAGESPRAP
                     ARDAITPPVAPAPRVALLPPAAFGSDPMLHAVSMAVVEDITIGLCALRSMSVVAPYTAE
                     RIRDAADKAAFLEKHAVTYALDCRMSDQGLFTQLIFLPSDAIVWAERFSISPVGLLQQR
                     QEIAFHVASAVAEQVETGRIGHLDYVTNPDAYYAYLAGLRNLSNVGLPEIRRARRDFKT
                     ALRHKPDFAPALSGIARTYAIEWVLTARGDQELLTVAERHAKEAIESDEELPGAHREFG
                     VVRLYQGDLDGSLAALDRAENLSPHYADVLYSHADTLVHASRPREALDKLGKALSLNPL
                     APDMYFWSAAGASYFLEEYEDAIGYVQKMKDKSPGDRLLAASWAMLGDQKKARSYKVKA
                     LRANPTFDVDKWLAVVPMKEQWQKDLYREGLKRAGF"
     misc_feature    8454..8467
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.78"
                     /tool="antismash"
     misc_feature    8464..8480
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.12"
                     /tool="antismash"
     misc_feature    8563..8578
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.43"
                     /tool="antismash"
     gene            8650..9441
                     /gene="aac(3)-IV"
                     /locus_tag="SmedWSM1115_RS12235"
                     /old_locus_tag="SmedWSM1115_12235"
     CDS             8650..9441
                     /codon_start=1
                     /gene="aac_3_-IV"
                     /gene_functions="resistance (resist) AAC3"
                     /gene_kind="resistance"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976343.1"
                     /locus_tag="SmedWSM1115_RS12235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12235"
                     /product="AAC(3)-IV family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="WP_018208010.1"
                     /transl_table=11
                     /translation="MTQREWRISELVNQLLTLGVKPGGVLVVHSSFRSVRPVEGGPAGM
                     IAALRTAIGPEGTLVMPSWSGLDDEPFEPATTSATSDLGILADTFWRLEGVVRSDHPFA
                     FAAAGPLAARITSDPLPLPPHCPPSPIGRIHELDGQVILLGVGHDSNTTLHLAELMSKV
                     PYGVPRHCTIVENGSRVRIAYLENDHCCQNFSLADVWLRRNGLQKEGQIGNAHARLVRS
                     RDIVKIAMEQLAHDPLVFLHPAEVACEECDAARQSIGYVRS"
     gene            complement(9426..9757)
                     /locus_tag="SmedWSM1115_RS12240"
                     /old_locus_tag="SmedWSM1115_12240"
                     /pseudo=""
     CDS             complement(9426..9757)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787770.1"
                     /locus_tag="SmedWSM1115_RS12240"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12240"
                     /product="helix-turn-helix transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPRWKKPTKEEVLENRRDLANRARSGELRLPHAVAEIRKGFGMTQ
                     EEFAKMLALTRRQVAEIEAGTANPTLETLEKMAVYSALGWVSCRGR"
     misc_feature    complement(9989..10003)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.28"
                     /tool="antismash"
     misc_feature    10219..10236
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            complement(10360..10800)
                     /locus_tag="SmedWSM1115_RS12245"
                     /old_locus_tag="SmedWSM1115_12245"
     CDS             complement(10360..10800)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013844676.1"
                     /locus_tag="SmedWSM1115_RS12245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12245"
                     /product="hypothetical protein"
                     /protein_id="WP_018012671.1"
                     /transl_table=11
                     /translation="MTFEVDTRTININTVVSVAGFLATFVFIGIAWGASQAALSDLEEW
                     RIQHEGVHRDLSGTIRSHDAVVDQQISTLRANLAKLDQIEYRITANEKGIEALDTRIGR
                     VTESYSNQFSDFRNQLSSISTQIALTNQTLQRIEAATPAAPR"
     gene            complement(10800..10982)
                     /locus_tag="SmedWSM1115_RS12250"
                     /old_locus_tag="SmedWSM1115_12250"
     CDS             complement(10800..10982)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013844677.1"
                     /locus_tag="SmedWSM1115_RS12250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_12250"
                     /product="hypothetical protein"
                     /protein_id="WP_018012672.1"
                     /transl_table=11
                     /translation="MIEALIALAITILVVGLIIFLIRRAPFIPAPFGQWAEYVVIVIAV
                     LIILLRALPLLGVSV"
ORIGIN
        1 atccgccctc ggtggcgaag ctcacctggg gcatggcgga actgccatcc gccgcatagg
       61 tgacgatcca gccgtgattc atcacgatct ccaggccgag cagggccctg tagaagcgat
      121 ctgcctcgag cagatcggat acctgaaaat taggcatgat gcgtcggact ttcatttgcc
      181 cctcccggct ggttgctgca cactcggttc cgaaagcgcc agctttacga gcgtagccgc
      241 ggcctcgccc gccgtctcca tatagctcgg atcacggtgg agcaaaacga ccgcgaagct
      301 gccgtcgagg agcagcagca tctgccgcgc gagcctggcc gcctctgcaa ttccttccgt
      361 ctcgaagctc tgacgcagcc agtcctcgaa cttccttttg tgcgctgccg ctatgcggat
      421 cgccgggtgg ccgggcatat tggcgagttc cgccgaagtg cggaggaaac cgcagccctt
      481 ccatttcggg tgccgggcag cccgggcgag attgagaaaa atggctttga tctttgacgc
      541 cgcatcaccg tcggtttcgc tgaaccactt ctggaagagc ttgaggttgg gctggtcacg
      601 ctcctcaaga taggcggcaa tcaactcgtc cttgctcttg aaatgatagt agagagtgcg
      661 cttcgtcaag ccggccgcct cggcaacggc gtccacgctg acggcgcgaa ttccttccgc
      721 atagaacagc ctggcagcgg cggaaacgat acgttcgcgt gtgttttgtg tcgccgccat
      781 aatccaggta taccgactag tgaatataca ttcctttgtc tcccacttag cctccgttcg
      841 tcaacacccg acgggagaca aacatgtccg atactgtgct tctgaaaatt tcgggcggaa
      901 ccgccctcct gacgctcaac cggcccgaaa ggctgaatgc gctcaattac gagctgatcg
      961 accggctttt gaccctcctc gatcgcctcg aggtggacgc caaggtgcat gccgtcatcc
     1021 tcaccggagc gggtgatcga gccttttcgg caggcggcga cattcacgaa ttctcccaca
     1081 gcgtggccga aggcgtgaac gccgcgatcc gcgatttcgt gcgaagaggg cagcagctta
     1141 ccagccgtct cgaggccttt ccgaagcctg tgatcgccgc cgtcaacggc atcgcctatg
     1201 gcggcggctg cgaaatcacg gaggcggtgc atctcgcgat cgcgagcgac cggtcgcgat
     1261 ttgccaagcc ggagatcaag cttggcatgc cgcccacttt cggcggcacg cagagactgc
     1321 cgcgccttgc cggacgcaag cgcgcgctgg agctgcttct caccggcgac gcgttttcgg
     1381 ctgagcgcgc gctggagatg gggctggtaa atgcggtggt tccgcacgcg gatcttatcg
     1441 agaccgcccg agcgctcgcg gcgcgcatta tcagccactc tcctgttgcg accgccgcta
     1501 tcatctcggc cgtgacgcgc ggcctcaaca tggcgatcgg cgaaggtctg ctttgcgaga
     1561 gcgagcaatt cgcacgaatg gttccgaccc acgacctcaa cgaaggcctc gccgcctgga
     1621 aagagcggcg cgagccgcgc tatgccggcc gctgagattg cccgatcatc aggtccgggc
     1681 gaagccgagg acgaagagcg ccagagcagc ggtcgaagcg aagctgcgca cgtggttcca
     1741 gcggacccat tcggtcagat agcgctgctg ccagagttcg gtggctcctt gtccggcagg
     1801 ggcggcggca agcgcttcgt tcagcggcac gttgacggct atcgtcaccg cgagcgtgcc
     1861 gatgagatag acgagtccgc cagcagccag gaaatagctc ccctgccctc cccagatgac
     1921 ggccatgacc aggagcacca ggccaagcag cgccgttccc atgaaggcga tgagaaacag
     1981 cgggttctgg atcaccacgt taatggcgtt catcacggcg acgccttgct cgggcgggag
     2041 tcgcgaaagc gcctgcataa tgcagaccga aaagacgaag aacagcccgg ccatcaggcc
     2101 ggatccgatc acggcggcaa atgcgagcac aggaacaagg gtaaccatcg caacactcca
     2161 ggagggaatt gagccgcttc gggtcgcttc tctcgtttca gacttgaagc agcgtcgacg
     2221 aaagccagcc tcccgcaaat ccagccctgc tcaacccctg acgaaaggcc ggaatgccgc
     2281 gcgtcagttc ccaaaccagg ccgctcctcc aattctcgat catcatgacg acgagccctt
     2341 ggtcaaggcc gacgagtcta tcgtccatcc atccctcggg accgtccccc ggcaggctgg
     2401 gattgaaacc gccgggaagg cgttcctcct tcaccagggc ggggtaacgc gacatcagat
     2461 gaaaaagact gccgagacac gcgtccggtt cgaatggcag cccggccagc ggcgcccagg
     2521 gaacgagtgt tccgtcgtcg gcaccaaacg gtgcgccgcg tgcctcgtag cccagcaact
     2581 tctgccgccg gccgtcgcga agccgaaacc agcctttcgg cgcatgacag gcgctgaaac
     2641 cccagaggtc gcggcaatag ccggcgaatc catgagggtt ttcctcggca tgggcgcgct
     2701 gcagccggat ggcgcggcgg gtgttatcga aatagtcggt ggccctgtcg cgcatcccct
     2761 tatcccggat gctgcgaaaa tcgatccatg cctgcggaaa aaggtggacg aagagcgcgg
     2821 cggcatggag atgcgccgcc ccatcgagtt ccagccactc atgcgctgcc gtgtaggcct
     2881 cgtagttcgg cggcatgatc gggcgcgtgg gcgagccgag tgccaggacg tagaggagca
     2941 gcgcctcgct atgaccccgc cagcggtgtt tcaaaaatcg ccccggcggg cgccacccca
     3001 tcgtgaccgt gtcgccgcca ttcagcgccc agtaccaatc cgccctctcg tagaggagcg
     3061 ccgcaagccg cctgagctca ctctcctctt cgctgcggcc agagaaataa gctgccgccg
     3121 tcaaggcacc ggcgaggagc aggctggtgt cgatcgtgga gagctcactg cgccagacgc
     3181 gatcccccgt ccgcatgtcg aggaaatggt agtagaatcc gcgataaccg gtcgcgctcg
     3241 catcgctccc ctgacggctt gccgcgaaaa accggagggt ggtcagcacg cgccaggcag
     3301 cgtccgaccg ggcgatccag ccgcgttcga ccgcgaccgg ataggccgca agtccgaaac
     3361 ccgtggcggc gatgctcgac ggagaacccg cgcgagacgt gtcggcgacg aggccgcttc
     3421 gcatgtccga ataccgaaga aagtattcga aggcggcgcg ctgcagcccg tcgatgcagg
     3481 cgatatccga tgtgcggctg tggagagaca tgccagccta ctggtcgacg aggttggtca
     3541 cattgcggca ggccacgcag accaaccgcg atcaccgaat attgtaggtc tgcatcgctc
     3601 cgacagtcgc cgatctcaaa acctgtgata cattcatgga atgacatcga cccggacggc
     3661 gacatcgctc taaggcgcct cgcggtaccc gggtgcaggg gcagaacgcc cgacgcgtga
     3721 tccacggtga ttggctgatg cgatgttagg cacgatggca ttgatcctgg cactgagcgg
     3781 agctccgata cagtcggcag ccatttccgc tgacgtcgac gtagaactcc tgctggccgt
     3841 cgacatgtcc ggctcgatgg acatggagga ggcgcgcgtg cagcgctccg gatatctgca
     3901 ggcgctgcgg catccggatt tcatcaatgc ggtccaggga ggcctcctcg ggcggatcgc
     3961 cataggctat ttcgaatggg cagggctcgt aaacgaggcc tcagtcgtga gctggcaagt
     4021 gatcgaaagt tctgccgacg ccgaagcctt cgccggcaag ctggaggctc ggccaatcgg
     4081 aacacggcgc ggcacctcga tctcgaacgc gatcctgttt ggttcggacc tcatcgattc
     4141 gaacgcatat tcgggtacgc gaagggtcat cgacatctcc ggcgacgggc cgaacaatac
     4201 cgggccgccg gttacacccg cacgcaacga cgccctatcg cggggcatta taatcaacgg
     4261 tcttgcaatc ctcatccgcc cttcggtctc gaccgggccg ctcgaccggt attacgccga
     4321 atgcgtcatt ggcggccccg gctccttcgt gttgcccgtg catgaacccg aggatttcgc
     4381 ggtcgcaatc cgtcagaagc tgatcctcga ggtaagcggg cgcgagcccg atccggcgct
     4441 ccgcctggcg gccggcgaaa atgcggcgga ctgcatgata ggcgagaagc tccggccggg
     4501 atttctcgac agggtctatc cggaactgga tcgctgacgc ttgctcttca tatccccacc
     4561 ttcctgtatg ccacaggaat ggtgaggacg aaagaagtgc ggtcgggtgc ggtagcagcg
     4621 caggcgggtt tccccgcagc gcgctattgc agcagggtcg ttctattgtc gaagcccgca
     4681 gcggcaatga cgagctcgcc gcgatcccgc aattcgttgg cgaccgcttg cagtgccata
     4741 tcgatgcaat actcgacggc ctgtgcctcc aaggcacgag cgctctcgcg aatgtggctc
     4801 aacattcggg caatgctcgc aagttccacc tcggtatccg tcaaggacga agaatgatta
     4861 gtcatatgaa cccccaatcc atgcctgtga tgagggcgac tataaacgag ttcggcattt
     4921 ccgcgcgttt cacgagcgtt gcacacgctc aggtggagta tttagcggat cttcgcccgg
     4981 agccccattg cccgctcccg tcacgatgcg agcgctttgg atagtgcacg cggaccaaaa
     5041 cggcgcgctc gatcaccatc cggattctcg agtttgggaa cgaagatctt gacgaccgag
     5101 aacggaagcg cgttcgccga cagacgaagc gcaattgcag attgcacgcc ggcgctcgtc
     5161 aggtgatcaa cggtgtggcg cagcagcgct tcaaggcttc cgccgacctg cgggctggcg
     5221 atggcccgtc tggatggctc ggcgaggaag gcctgccgcg tcagttccgg caagggccgg
     5281 ttgaaggtct cgggaaagac atcgtcccgg gcaccactga tgaaggtgag ccgggactgc
     5341 gccgcttctg tcaccgccct gatcgccgcc cgggcgggaa aaacatgtgc gccgcagccg
     5401 ctcgtcactt cgacgaagcg gccaccgccc ctggtcgcga tatcgggtgg gccgagcagc
     5461 gccaggaaac agggaatgcc tatgtcgctg gtgatttcga aaagccggag atcgagtccg
     5521 gcggccccaa tgcgctcaac gagaccgttc aacgctgcat cgcccaggct ctcgggagcg
     5581 acacagcacc cgtaacggtg ctcccgcccc gagatgcccc agaggacgtg cgcgtctcgc
     5641 tcgatgcgct ccagcagtcc gtgcaatatc gcttcttcca cgacgtttcc cgacgcaagt
     5701 ccatccgacg acatccagaa acgcgaatgc agggtacggt cgagcagcgc cgctgcgagc
     5761 ggcacgagta tctcgcggcc ggagatcagg tcggagccga gggcccatgc gagttcttcg
     5821 tccggcccta tatcctgctg cccggccgca atcaggcagg gaagcagctc cacggtccgt
     5881 ccggatgcac gcaaggtccg ggccgtggcg accattgttc cgacggcggg ctcgcaggca
     5941 acggcgcgct ccagcgcctc catgaccgcg gaaaccttcg cctccggatc cgtcaatccc
     6001 ttgccctgcg caaccgcgat cgatcgtgcg ttcggcgtgt aggcgcacca gaccgggagc
     6061 ccgatcctgt cgaggcccgt gtgccgtgcg atgcgggtca cgccgaaccg ggaaagcagt
     6121 ggccggacac ggtcgaacgt ctcttccggg ctcagaacgc gatcggaata agcattgcgc
     6181 gtgtctgcag gactgccgac agcgaagccg atcacccgtc gaaatgcccc gaaaacacct
     6241 tgtcggccga cgacaccgct actgcgagat caacattctt gacgatcgtc ccgccctgca
     6301 ggcggagctt gttcgccgtg gcgagatcgc ccgacacctg atcgagcggc ctgaccgttc
     6361 catttttcag atccgccagc cacaaacctt gctcgccggc aataccaatt actacgactt
     6421 ctgccatatc gcttcccctc atcatcaaaa ccccgctctt ttcagcccct cgcgatagag
     6481 gtccttctgc cattgttcct tcatcggaac gacggcgagc cacttgtcga catcgaatgt
     6541 cggattggcc ctcagggcct tcaccttgta tgaccgcgct ttcttctgat cgccgagcat
     6601 cgcccagctt gctgcaagca gccggtcgcc cggcgacttg tccttcattt tctgaacgta
     6661 gccgatcgca tcctcatact cttcgagaaa gtagctggct ccggcagcgc tccagaaata
     6721 catgtccggc gccaggggat taagcgaaag cgccttgccg agcttgtcga gagcctcacg
     6781 cgggcgggag gcatggacga gcgtgtccgc atggctgtac agcacgtcgg cataatgcgg
     6841 gctcagattt tcggcgcggt ccagcgccgc aagacttcca tcgaggtcgc cctgataaag
     6901 tctgacgacg ccgaattcgc gatgtgctcc gggaagctct tcatcgctct cgatcgcttc
     6961 cttggcgtga cgctcggcta ccgtcaaaag ctcctggtcg ccccgagccg tcaggaccca
     7021 ctcgatcgca taggtgcgtg cgatgccgct tagcgccggg gcaaaatccg gcttgtgcct
     7081 gagcgccgtc ttgaaatcgc ggcgtgccct gcggatttcg ggaaggccga cgttcgaaag
     7141 gtttcgcagg ccggcgagat aggcgtaata ggcgtccgga ttggtcacgt aatcgaggtg
     7201 cccgatacgc ccggtctcca cctgttccgc taccgcgctg gccacgtgaa aggcaatttc
     7261 ctgacgctgt tgcaggagcc cgaccggaga gatcgaaaag cgctcggccc agacgatcgc
     7321 gtcggacggc aggaagatga gttgagtaaa cagcccctgg tccgacatgc gacagtcgag
     7381 cgcataagtg acggcatgtt tttccaggaa ggcagccttg tccgccgcat cccgaatacg
     7441 ctcggcagtg taaggggcaa caaccgacat cgagcgcagc gcgcataatc cgatcgtgat
     7501 gtcttcgacc acggccatgc tgaccgcgtg aagcatcgga tcactgccga aggccgcggg
     7561 cggaagcagc gcaacgcgcg gtgcgggcgc aaccggcggg gttatcgcat cccttgccgg
     7621 cgcccggggc gactcccctg caaaggcccc ctgccgcagc caggccccgt ccggcgagcc
     7681 tttcccgatg acgagaccca cgccggtcaa ccgctccagg attgcgcgca cggcagtatc
     7741 cgccggatcg agttcgagga gatgcatgca cgccgtccgc agcatcgacc tgtcggcaaa
     7801 tcgctctccg ccggctaaaa gtgccgcgcg cagcttttgc gtaaacacgc tccgctgctc
     7861 tccaacccag tccgcataac gcccgggagg cacatcggcg cctgccagga acgtatcgcg
     7921 ccagagcgag agcccggcgc gaaacgcttc ctccgagctc ccgagcgcct cgaaaccggc
     7981 ggcgtcgcag gcaatctccc ccagcgtcac atctatttcc gtcgccgacg tgagcggccc
     8041 cgagttcgcg gcaaggtggc gcatgcgcca cagggtagag cgcaggttct tgaaagcgac
     8101 gctcttctcc cgctcgggcc agatcagcat cgagagcttt tcgcgggaga ccgttctctg
     8161 cccggatatg ctgaggtagg ccagaataag caggccccgc tccgggcacg ccacgaccac
     8221 gccgccggaa tcgaccagcc gcaattcccc gaatgtctgc agatgaaaat gcggcaccgc
     8281 cacgaaaacc ccaaacggct ttagatcaat atgattttag gtcaccaccg gccgccgccg
     8341 gcgaatgcat cgaaatctcg ttcacccagc cctcttccac cccttagggc aatcacacct
     8401 cggtccgcag agcggatagc cagtgtgcgc ggttttcagc ccagatgcag ccatgcatga
     8461 acatgcttga aaaggcggaa catggcaacc gatgccgctt atagctccca aggggaacct
     8521 gaaagcgccg acggagctcc ccattattgt tccacgcccg cctcaacgct attttcaacg
     8581 cgttcgaagc cgataccgtg agcaagcgct gtcagaacac caattaaagt ggactcatcc
     8641 ggtacaacca tgacgcaacg tgaatggcgc atttcggaac tcgtcaatca actcctcacc
     8701 ctcggggtga agcccggagg cgttcttgtc gttcactctt cgtttcgcag cgtccggccg
     8761 gtcgaaggcg gtccggccgg catgatcgcc gctctgcgca ccgccatcgg cccggaaggc
     8821 acgctcgtca tgccctcctg gtcgggcctt gacgacgagc cattcgagcc ggcgacgacc
     8881 tcagcaacat cggatctggg aatactcgcc gatactttct ggcgtcttga gggtgtggtg
     8941 cgcagcgacc atccgttcgc ctttgcagcg gcgggaccgc ttgccgcacg catcacatcg
     9001 gaccccctgc ccctgccccc gcattgtccc cccagtccga tagggcgtat ccatgaactc
     9061 gacggacagg tgatactcct cggcgtcgga catgattcga acaccacctt gcatctcgcc
     9121 gagctgatgt ccaaggttcc ctatggagtg ccgagacact gcacgatcgt cgaaaatggc
     9181 agccgggtcc gcattgccta tctggagaat gatcactgtt gccagaactt cagcctggcc
     9241 gacgtctggt tgaggcgaaa cggcctccag aaggaggggc agatcggcaa cgcccatgcc
     9301 cgcctggtcc gctcacgcga catcgtgaaa atcgcaatgg aacaattggc ccacgacccg
     9361 ctcgtcttcc tgcatccggc agaagtggcg tgcgaagaat gcgatgcagc acgccagtcg
     9421 atcggctatg tgcgttcgtg agagcctttc ggcgccggat cacgctcgct ctcagttttc
     9481 tttatcgtcc ccggcacgaa acccacccca aagccgaata gacggccatt ttttccaatg
     9541 tctccaatgt cgggttcgct gtgccggctt cgatttcggc aacctgcctc ctggtgagcg
     9601 ccagcatctt cgcaaactcc tcttgcgtca tgccgaagcc cttgcgaatt tcagcaacgg
     9661 catggggcaa gcgcagctca ccgctacgcg cacggttcgc cagatcgcgt cgattttcca
     9721 ggacttcttc tttggtcggc ttcttccagc ggggcatcag cggtctccgt tccggctcag
     9781 gatagcgaca ccagaacgct ctcggcgatt tccgcgcagc gccccatgcc gcgatcaacg
     9841 caggaatggc gcagcagcac tatacgaagc cgtgcggacc cgagcgcatc atcgattcag
     9901 gtttcgagcg ctgcaagaca tatgtggccc gggaggtttc gctttcgcct aagcagaatt
     9961 ccggcgagac agacccagga aaatgaagga aaaacgacgt tcaatgaggg aatggtgggc
    10021 ccggaggaag ggccagaacc attgaaaaca ttgcagaatt cgtgggcaaa acgccgatgg
    10081 tttcccgcaa tgttccgttt ttgcccacgc gcccctgtgg gcaaacgccc ccaatcaccc
    10141 ccctataaac cagaatcccc gccagcttcc cgcccgtcga ggcgcgcagc gcgcttggcg
    10201 cccatggcat gcctaatcta tcccggcgat cggaaacgcg cgtcagcggc ccgctatggc
    10261 cgaaacaaaa aaagcccgcc ccggcgcgag gccaagggcg ggacagttgg gcgcgggccg
    10321 ccgggcgagg tttgcctagc ccgcgccggg aggaaatgat cagcgcgggg cggccggcgt
    10381 cgccgcctcg atccgctgga gggtctgatt ggtcaacgcg atctgcgtcg atatcgatga
    10441 cagctgattg cggaaatcgg agaactgatt gctgtagctt tcagtgaccc ggcctatccg
    10501 ggtatcgagt gcctcaatgc ctttttcgtt cgccgtaatg cggtattcga tctggtccag
    10561 cttggcgagg ttggcgcgga gggtcgaaat ctgctgatcg accacggcat cgtgcgagcg
    10621 gatcgtcccc gataaatcgc ggtgcacgcc ttcatgctga atgcgccatt cctccagatc
    10681 ggacagcgcc gcttgcgagg cgccccatgc gatgccgata aagacgaacg tcgcgaggaa
    10741 cccggcgacc gaaaccaccg tgttgatatt gatcgtccgg gtatcgactt cgaaggtcat
    10801 cacacgctga ctcctagcag cggcagcgcc cgcagcagga taatcaagac ggcgatgacg
    10861 atcacgacat attcggccca ttgaccgaac ggcgcgggga tgaatggcgc ccgccggatc
    10921 aggaagatta tcagcccgac gacgagaatc gtaatcgcca aggcaatcag tgcctcaatc
    10981 attgcggcct cctatttcca gcacttctgc gcgcgcccga aagcgttggt gctggcaacg
    11041 cctcggccgg taggaacgag atcgagatcg ctggccagct taacggcgcc ggccggggtg
    11101 acgggtatct tcctccaccc cgcgcaattg ctggcattcg tcgtgcaccc cgccgccaag
    11161 aagctcattg caaagagcga tgccgtccaa gccttgaatc cgttcatcga ttttc
//
