LOCUS       NZ_CP088109            20843 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Sinorhizobium medicae WSM1115 chromosome, complete genome.
ACCESSION   NZ_CP088109
VERSION     NZ_CP088109.1
KEYWORDS    RefSeq.
SOURCE      Sinorhizobium medicae WSM1115 (Ensifer medicae WSM1115)
  ORGANISM  Sinorhizobium medicae WSM1115
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
REFERENCE   1  (bases 1 to 4106266)
  AUTHORS   Kohlmeier,M.G., O'Hara,G.W., Colombi,E., Ramsay,J.P. and
            Terpolilli,J.
  TITLE     Australian commercial rhizobial inoculants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4106266)
  AUTHORS   Kohlmeier,M.G., O'Hara,G.W., Colombi,E., Ramsay,J.P. and
            Terpolilli,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2021) Legume Rhizobium Sciences, Murdoch
            University, 90 South Street, Murdoch, WA 6150, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 149x; 384x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 05:22:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,835
            CDSs (total)                      :: 6,766
            Genes (coding)                    :: 6,456
            CDSs (with protein)               :: 6,456
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 205 of 310
            Pseudo Genes (incomplete)         :: 185 of 310
            Pseudo Genes (internal stop)      :: 32 of 310
            Pseudo Genes (multiple problems)  :: 101 of 310
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:56:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1293932
            Orig. end                         :: 1314775
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP088109.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20843
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="2"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20843
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20843
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10843](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10843
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            903..1673
                     /gene="surE"
                     /locus_tag="SmedWSM1115_RS06195"
                     /old_locus_tag="SmedWSM1115_06195"
     CDS             903..1673
                     /EC_number="3.1.3.6"
                     /GO_function="GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="surE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969283.1"
                     /locus_tag="SmedWSM1115_RS06195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06195"
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="WP_018208605.1"
                     /transl_table=11
                     /translation="MRILLTNDDGIHAEGLSVLERIARTITDDVWVVAPEVDQSGLAHS
                     LTLSEPLRLRPVSERRFALRGTPTDCVIMAVKKILDRKPDLVLSGVNIGANLADDVTYS
                     GTVAGAIEGTLQGVRSMALSQAYHYAVGREVPWDVAETHAPALIRTLMDVDLPDGTLVN
                     LNFPNCAVDSVAGVEVTSQGKLDFGLSIDERTDGRGFPYFWLRFGERAGDFRSGTDIRA
                     LRDNKISVTPLKLDMTDHAVQDLIARALRKGTVA"
     gene            1670..2323
                     /locus_tag="SmedWSM1115_RS06200"
                     /old_locus_tag="SmedWSM1115_06200"
     CDS             1670..2323
                     /EC_number="2.1.1.77"
                     /GO_function="GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526709.1"
                     /locus_tag="SmedWSM1115_RS06200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06200"
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="WP_011975339.1"
                     /sec_met_domain="PCMT (E-value: 2.8e-38, bitscore: 129.6,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNARVSQQEGFAAMVLRLRSGGIVNHELLKAVEQTPRLLFAPPQY
                     QDEVYSKRLIPLDCGSFMEGCDMAVRLLHCLNLKSGQRILEVGTGSGFTAAVMGRIAER
                     VLTIDRYQTLVASAQKNLEKAGIRNVVVRQADGSAGVPGEGTFDRILITAAFNSLPRMF
                     SDHLVSGGTLLVPIMMSETHCRIVRVNRTGSRFDREDLFDAPYLPIVPQVASFL"
     gene            2486..3136
                     /locus_tag="SmedWSM1115_RS06205"
                     /old_locus_tag="SmedWSM1115_06205"
     CDS             2486..3136
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097725.1"
                     /locus_tag="SmedWSM1115_RS06205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06205"
                     /product="biotin biosynthesis protein BioC"
                     /protein_id="WP_018208604.1"
                     /transl_table=11
                     /translation="MQVGNRLNAAAAAGDGLGNLAGRPADQTGAASKSESGTQLPPASF
                     LDPTPRISLSADALLYLGRTKRTLEKLPPLTKDEWNNHLSPQLAAREHQAFGKFAQTAD
                     YRAYYRGFIDYYDNLRPEDQNSLRYFGTREAAVAGLRSLEYDVESGLDIGANFEGLVSV
                     FLEENKTALSPATAPPPAERAYFAWDASNISYEHEAPEPRLMTEIERLYSELI"
     gene            3372..4904
                     /locus_tag="SmedWSM1115_RS06210"
                     /old_locus_tag="SmedWSM1115_06210"
     CDS             3372..4904
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969286.1"
                     /locus_tag="SmedWSM1115_RS06210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06210"
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="WP_018208603.1"
                     /transl_table=11
                     /translation="MRKFESPQAGKSMIRLCAAILLAGVATGCSSDASRFGGLFSRSDD
                     IMTGSIPQGSSTMPRGDVASGGAAPSYGNGASVGQSYPAGGGGYNAAAAPVSSARVAST
                     PMAVQRTSLDEPSATSGQQQVRTASLDSQAAALPRSQPQSGGARDIPSKGGWSASNAPT
                     ILVRQGDTVTVLARRFGVPEKEILRANGLKSSSQVEPGQRLVIPTFGAAGSAAKAAASG
                     SIADVEGGKRRPSPLPTDQREVAILPGQSQSREKNESRSDVAAGKLNSAGEGGGTGGYA
                     VKPGDSLNRIAKANGVSVAALKQANGLTTEAIRIGQKLNIPSASANTPATDAVVTASVS
                     PKKNEAKVAATEQSKPAEAKAAAAKESVSEVAIKSDVNEDLPKSTGIGKYRWPVRGAVV
                     AAYGANVDGNRNDGINISVPEGTPIKAAENGVVIYSGSSLKELGNAVLVRHDDGTVTVY
                     GNAAELKVQRGQKVQRGQTLASSGMTGRASRPQVHFEVRKNATPVNPVTYLE"
     gene            complement(5308..6183)
                     /locus_tag="SmedWSM1115_RS06215"
                     /old_locus_tag="SmedWSM1115_06215"
     CDS             complement(5308..6183)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011013.1"
                     /locus_tag="SmedWSM1115_RS06215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06215"
                     /product="ATP-binding protein"
                     /protein_id="WP_011975342.1"
                     /transl_table=11
                     /translation="MPESKIDVLLDEIHRLSAALERIAGPAYAVNDWNEADCFVWAPAT
                     RHLQPVPKPNRIDLALISGVDHVRDILFDNTLRFAQGYPANNVLLWGARGMGKSSLVKA
                     VHAKVAHDADSALKLVEVHREDIATLPVLMEILKAAPVPVIVFCDDLSFDHDDTSYKSL
                     KAVLDGGVEGRPANVLLYATSNRRHLLPRNMMENEQSTAINPSEAVEEKVSLSDRFGLW
                     LGFYKCSQDDYLAMVDGYAQYFKLPVEPETLHAEALEWATTRGSRSGRVAWQFIQDLAG
                     RLRRQLDTPA"
     gene            6520..6852
                     /gene="yajC"
                     /locus_tag="SmedWSM1115_RS06220"
                     /old_locus_tag="SmedWSM1115_06220"
     CDS             6520..6852
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /gene="yajC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241187.1"
                     /locus_tag="SmedWSM1115_RS06220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06220"
                     /product="preprotein translocase subunit YajC"
                     /protein_id="WP_011975343.1"
                     /transl_table=11
                     /translation="MFITEAFAQTAAPGGGGADILMSILPFLLIFVVMYFLIIRPQRAQ
                     MKRREELLKNIRRGDQVVTGGGIVGKVTKVVDDTELEVEIAEGTKVRVVRSGVSEVRVK
                     GEPVKE"
     gene            7014..9590
                     /gene="secDF"
                     /locus_tag="SmedWSM1115_RS06225"
                     /old_locus_tag="SmedWSM1115_06225"
     CDS             7014..9590
                     /codon_start=1
                     /gene="secDF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013844336.1"
                     /locus_tag="SmedWSM1115_RS06225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06225"
                     /product="protein translocase subunit SecDF"
                     /protein_id="WP_011975344.1"
                     /transl_table=11
                     /translation="MPRFSPFKNILIWLVVLAGFACALPNLLSREQLADWPTWLPHRQV
                     PLGLDLSGGSHIVLQVNREDIVAERLHSTIDAIADALRQADIHYTGLSGSGRSLQFRLR
                     DGTKAPAAREALKALSAPVQQDGLFGKSIQELVYEESATNDTLRLRLTDEGIDFRVSAA
                     VARSVEVVRRRVGEVIAVAPSIERRENDRLSVQVPGLDEPQRLKDLLAQRGDVALRWLD
                     LSMPAQQAVDTRPPAASEVLYSLDDPPIPYLVERRAFAAAPDFVEAQPGFDPATAEPVV
                     DVRIAAGATERIAPILQSDPRKQFAIVLDGQVISTSVLDSAVSGDAVRISGNLSEDGAN
                     DLAALIRAGPLPVSLTVIEERTVGPEDGADAVANGLTAGLIAALAVAACMIGFYGFFGL
                     VAVVAVIVNVVLIIAVLSVTGATLTLPGIAGIILTIGIAVDSNVLIYERLREEARSSDK
                     PLRRALDDGFERVLRSIVDSNLTIFIAGVILLSLGSGAFSGFAVTLAVGSLTTILTAHS
                     LTRRLIRGWYRRRRPHRLPRGIRTGFFSRADFRFMAIRNPVFILMTALSLASLVLLTSL
                     GLNMGADFRGGSVIELRARTGVADRADIRARLDELNLGEVQIEELGSARDVLIRIPSQE
                     AGDNAEQTAVGLVRAELEDQYDFRRVAVVGPGVSGELTRAGSLALAAAVLSVLLYVWLR
                     FGRRFALGAVVATLHDVLLTLGFLGLTGIEFNLASIAALLTIVCYSLSDTMVIYDRVRE
                     NLVRYRRMPLSVLIDTSINQTLSRTVLTSLTTMLALVALYLFGGSVLVQTFSATLIFGV
                     LVGTVSSIYVAGPLLILFNRRNSRLGGTEDPNNPDSPSKTEAKGAV"
     gene            9593..9979
                     /locus_tag="SmedWSM1115_RS06230"
                     /old_locus_tag="SmedWSM1115_06230"
     CDS             9593..9979
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529512.1"
                     /locus_tag="SmedWSM1115_RS06230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06230"
                     /product="Mth938-like domain-containing protein"
                     /protein_id="WP_011975345.1"
                     /transl_table=11
                     /translation="MAKGIEMREAHFPGRAPVDAYGNGGFRFADMSHRGSVLMLPSGIY
                     AWGVAEGDLLAVAKFQRVFDEAPGIEVLLVGTGLEIRPLPVELKAALRAAGISSDPMST
                     GAAVRTYNVMLAESRAVAAALIAV"
     gene            10001..10843
                     /locus_tag="SmedWSM1115_RS06235"
                     /old_locus_tag="SmedWSM1115_06235"
     CDS             10001..10843
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529514.1"
                     /locus_tag="SmedWSM1115_RS06235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06235"
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="WP_011975346.1"
                     /sec_met_domain="PT_phytoene_like (E-value: 1.3e-14,
                     bitscore: 52.0, seeds: 61, tool: rule-based-clusters)"
                     /sec_met_domain="phytoene_synt (E-value: 2.6e-21, bitscore:
                     73.9, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQQSDDQILATLRDTDRDRYLACLLSPVEKRHSLAALYAFYAEIA
                     RVRDMVKEPLPGEIRLQWWRDILENEKSDGGGHPLAEALLRCVREHQLPVRVLQDMIDA
                     RIFDLYEDPMQDRAALEGYAGETASALIQLSSLILDPVNAAESADAAGHAGVAQTIAGL
                     LLLLPVHVGRGQVYLPAEILSATGLDREALLAVNDEGAVENAIRAFCGLGRDHLARARK
                     SASSISARNFSAFIPVALAEPVFDRAEAAGARILRQPLQAPQWQRQWRMWRASRRRKF"
     gene            10925..11380
                     /locus_tag="SmedWSM1115_RS06240"
                     /old_locus_tag="SmedWSM1115_06240"
     CDS             10925..11380
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241188.1"
                     /locus_tag="SmedWSM1115_RS06240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06240"
                     /product="hypothetical protein"
                     /protein_id="WP_011975347.1"
                     /transl_table=11
                     /translation="MLWLLFPAAIAAVCVFYIRMNARAERDADNPDAGSAIVEFGRAFP
                     DEAIRALHSSVDGTAIFLRLHDGKAGFIQSHGTHYVCHVVQPDKVRVNTSDSGRGLIVH
                     FPDFAYLDNAFEFRTAAEAAEVSLWLLGSFRTKLEKEASGNAAPGPD"
     gene            complement(11390..12832)
                     /gene="trmFO"
                     /locus_tag="SmedWSM1115_RS06245"
                     /old_locus_tag="SmedWSM1115_06245"
     CDS             complement(11390..12832)
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_process="GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="trmFO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011009.1"
                     /locus_tag="SmedWSM1115_RS06245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06245"
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="WP_018208601.1"
                     /transl_table=11
                     /translation="MNKLTSSYSPVHVIGGGLAGSEAAWQIAEAGVPVILHEMRGVRGT
                     DAHKTDSLAELVCSNSFRSDDATSNAVGVLHAEMRLAGSIIMRCADLNQVPAGGALAVD
                     REGFAEAVSAAIAAHPLITVLREEIRGLPPKEWDLAIIATGPLTAPDLADAIRAETGAD
                     ALAFFDAIAPIVHADTIDMDICWHQSRYDKVGPGGTGKDYINCPLKQEEYNAFVDALIA
                     GDKTGFKEWEGTPYFDGCLPIEVMAERGRETLRHGPMKPMGLTNAHNPSVKPYAVVQLR
                     QDNALGTLYNMVGFQTKLKYGAQGEVFRMIPGLEKAEFARLGGLHRNTYINSPTLLDQS
                     LTLKSRAGLRFAGQITGCEGYVESASIGLLAGRFAAAERKGASPAIPPVTTAFGALLNH
                     ITGGHIVSDDEPGKRSFQPMNVNFGLFPPLDPGALTRPEGAKRFRGKEKASAKKQAMAS
                     RALSDCATWLDKNLLIGGAATR"
     gene            complement(12829..13041)
                     /locus_tag="SmedWSM1115_RS06250"
                     /old_locus_tag="SmedWSM1115_06250"
     CDS             complement(12829..13041)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /locus_tag="SmedWSM1115_RS06250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06250"
                     /product="hypothetical protein"
                     /protein_id="WP_231234239.1"
                     /transl_table=11
                     /translation="MDQLPPHRCRTRQPFEPRPERYRHHSLRYPQHRGPLLPLTGRALH
                     SLKTAPSGAVFVSWKRRSAVVGSRA"
     gene            complement(13363..13506)
                     /locus_tag="SmedWSM1115_RS06255"
                     /old_locus_tag="SmedWSM1115_06255"
     CDS             complement(13363..13506)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529522.1"
                     /locus_tag="SmedWSM1115_RS06255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06255"
                     /product="DUF1127 domain-containing protein"
                     /protein_id="WP_003529522.1"
                     /transl_table=11
                     /translation="MNLARSFNNWRKYRQTCNELGRMSDRELTDLGIGRADIPYVARQA
                     IK"
     misc_feature    complement(13588..13604)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.7"
                     /tool="antismash"
     misc_feature    14009..14025
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.52"
                     /tool="antismash"
     tRNA            14509..14593
                     /anticodon=(pos:1308475..1308477,aa:Leu,seq:tag)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SmedWSM1115_RS06260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SmedWSM1115_06260"
                     /product="tRNA-Leu"
     gene            14509..14593
                     /locus_tag="SmedWSM1115_RS06260"
                     /old_locus_tag="SmedWSM1115_06260"
     gene            14769..16244
                     /gene="tig"
                     /locus_tag="SmedWSM1115_RS06265"
                     /old_locus_tag="SmedWSM1115_06265"
     CDS             14769..16244
                     /EC_number="5.2.1.8"
                     /GO_component="GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA]"
                     /GO_function="GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /GO_function="GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /GO_process="GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /gene="tig"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012651321.1"
                     /locus_tag="SmedWSM1115_RS06265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06265"
                     /product="trigger factor"
                     /protein_id="WP_018208599.1"
                     /transl_table=11
                     /translation="MQVIETLAQGLKRELKVVIPADEMEARMNERLVEVKDRVRINGFR
                     PGKVPVAHLKKVYGKSIMADLVNEIVREKPTEILTSRGEKSATQPEIGMTEDEAEADKI
                     LKAEADFEFTVAYEIIPPIELKDASGIKVTREVVDVGEDEVNEQIERIAESARTYESKK
                     GKAANGDRVTIDYLGKVDGEAFDGGKDEDAELVLGSNRFIPGFEEQLVGAKAGDEKTIT
                     VTFPADYPAANLAGKEATFDVTVKDVAAAAPIEINDELATKLGLESVDKLKEIVRGQIE
                     SQFGSITRQKVKRQLLDQLDELYQFDTPERLVDAEFENIWRQINTDLQQAGKTFADEDT
                     TEEEARAEYRKLAERRVRLGLVLSEIGEKAGVQVSDDEMQNSLFQQLRQFPGQEKEIIE
                     YFRNTPGAAASLRAPLFEEKVVDHLLTEVSVTDKKVSKEELTAEDDADEKPAKKTASKK
                     KAAAKADAAEGEEAAAPKRKAPAKKKASDESAE"
     gene            complement(16634..19498)
                     /gene="gcvP"
                     /locus_tag="SmedWSM1115_RS06270"
                     /old_locus_tag="SmedWSM1115_06270"
     CDS             complement(16634..19498)
                     /EC_number="1.4.4.2"
                     /GO_function="GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA]"
                     /GO_process="GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /NRPS_PKS="Domain: Beta_elim_lyase (535-679). E-value:
                     3.2e-09. Score: 28.4. Matches aSDomain:
                     nrpspksdomains_SmedWSM1115_RS06270_Beta_elim_lyase.1"
                     /codon_start=1
                     /gene="gcvP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017960600.1"
                     /locus_tag="SmedWSM1115_RS06270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06270"
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="WP_018208598.1"
                     /transl_table=11
                     /translation="MSMPKDFTFTDYKPYDFANRRHIGPSPAEMDEMLKVVGYPSLDAL
                     IDDTVPPSIRQQTPLAWGAPMTEREALDKLRETANRNRKVVSLIGQGYYGTITPPVIQR
                     NILENPAWYTAYTPYQPEISQGRLEALLNFQTMVCDLTGLDVANASLLDEATAAAEAMA
                     IAERVAKSKAKAFFVDENCHPQTIALLKTRAEPLGWQIVVGNPFEDLDAAIVFGAIFQY
                     PGTYGHVRDFSGLIARLHELGAIAAVAADPLALALLKSPGEMGADIAVGSTQRFGVPVG
                     YGGPHAAYMAVRDAYKRSMPGRLVGVSVDARGNRAYRLSLQTREQHIRREKATSNICTA
                     QVLLAVMASMYAVFHGPEGIKAIAQSVHQKTVRLALGLEKLGYTVEPDVFFDTITVEVG
                     KLQGIILKAAVAEDVNLRKIGTTKIGISLDERSRPITLEAVWRAFGGDFSVDQFEPDYR
                     LPKELLRTSDYLTHPIFHMNRAESEMTRYMRRLADRDLALDRAMIPLGSCTMKLNATAE
                     MLPITWPEFSEIHPFVPADQAMGYHHLIEDLSQKLCAITGYDAISMQPNSGAQGEYAGL
                     LAIRAYHIANGNEHRDVCLIPTSAHGTNPASAQMAGMKVVVVKVSDAGEIAMDDFRAKA
                     EQYAETLSCCMITYPSTHGVFEENVREVCEIVHKHGGQVYLDGANMNAMVGLSRPGDIG
                     SDVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFLPGHPESGEHEGAVSAAPFGSASI
                     LPISWSYCLMMGGEGLTQATKVAILNANYVAARLKGAFDVLYKSAKGRVAHECIIDTRP
                     LAESAGVTVDDVAKRLVDCGFHAPTMSWPVAGTLMIEPTESETKAELDRFCDALLAIRE
                     EARAIAEGRMDKINNPLKNAPHTVEDLVGDWDRPYSREQACFPPGAFRVDKYWSPVNRV
                     DNVYGDRNLVCTCPPIESYAEAAE"
     aSDomain        complement(17462..17893)
                     /aSDomain="Beta_elim_lyase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SmedWSM1115_RS06270_Beta_elim_ly
                     ase.1"
                     /evalue="3.20E-09"
                     /label="SmedWSM1115_RS06270_Beta_elim_lyase.1"
                     /locus_tag="SmedWSM1115_RS06270"
                     /protein_end="679"
                     /protein_start="535"
                     /score="28.4"
                     /tool="antismash"
                     /translation="DQAMGYHHLIEDLSQKLCAITGYDAISMQPNSGAQGEYAGLLAIR
                     AYHIANGNEHRDVCLIPTSAHGTNPASAQMAGMKVVVVKVSDAGEIAMDDFRAKAEQYA
                     ETLSCCMITYPSTHGVFEENVREVCEIVHKHGGQVYLDGA"
     gene            complement(19498..19860)
                     /gene="gcvH"
                     /locus_tag="SmedWSM1115_RS06275"
                     /old_locus_tag="SmedWSM1115_06275"
     CDS             complement(19498..19860)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /gene="gcvH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529528.1"
                     /locus_tag="SmedWSM1115_RS06275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SmedWSM1115_06275"
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="WP_011975352.1"
                     /transl_table=11
                     /translation="MLKFTEEHEWLKIDGDVATVGITEHAAGQLGDLVFVELPEAGSTF
                     SKGDTAATVESVKAASDVYCPLDGEIVEANQAIVDDPSLVNSDPQGAAWFFKLKLADPD
                     AAKALLDEAAYKELVA"
     misc_feature    19885..19898
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 20.74"
                     /tool="antismash"
ORIGIN
        1 tcgcgcttca cgatcctcaa aggtcagctc gcgcgcctgg aacgcgcgct cggccagttc
       61 atgctcgacc tacacacagg ggaacatggg tacatcgagg ttcagcctcc gttgctcgtc
      121 cgcgacgatg cgatgtatgg caccggccag ctgccgaaat tcactgatga tctcttccgc
      181 acgactgatg gacgctggtt gatcccgacg gctgaagtcc cgctgacgaa catggtccgg
      241 gaacagatcc tcgacggcga aaagctgccg ctgcgcttta cggccttgac cccatgcttc
      301 cgctccgagg ccggctccgc cggccgtgac acccgaggta tgttgcgcca gcatcagttc
      361 aataaagtcg aactggtgtc gatcacggat gccgagagcg cgctcgacga acacgagcgg
      421 atgactgctt gtgccgagga ggtacttaag cgtctcggcc tgcattaccg tgtgatgacg
      481 ctttgcaccg gcgacatggg atttagcgct cgcaagacgt acgatctcga ggtctggctg
      541 ccgggacagg acgcctatcg cgagatttct tcgtgctcgg tctgcggcga ttttcaaagc
      601 cgccgaatga acgcgcgtta ccgcggaaaa gaagaaaagg ggacgaagtt cgcccacacg
      661 ctgaacggtt ccggcgtcgc cctcggccgg gcgctgatcg ccgtcatcga gaattacctg
      721 aatgaggacg gctctatcac cgtgccagac gccctcatag cctacatggg cggcctgcga
      781 cggatcgaga agactgctta gggcaggccc agctttgtga aatcgagacc tgccggagca
      841 gagaacccgt tagacggttt tccgggaagt gctccggcac cataaatgga agaagaccgg
      901 caatgcgcat cctgctgacg aacgacgatg gcattcacgc ggaaggtctt tccgtgctcg
      961 aacggatcgc tcgaacgata accgacgatg tttgggtcgt cgcgccggaa gtggaccaga
     1021 gcgggcttgc ccattcgctg acgctttcgg agcccttgcg cctgcgcccg gtttcagaac
     1081 ggcggtttgc gctccggggc acaccgacgg attgcgtgat catggcggtg aagaagatcc
     1141 tcgaccgaaa gcccgatctc gttctttccg gcgtcaatat cggcgccaac cttgcagacg
     1201 acgtaaccta ttccggaacc gtggcgggcg cgatcgaagg cacgttgcag ggggttcgct
     1261 cgatggccct cagccaggca tatcactacg ccgtcggccg ggaggttccc tgggatgtgg
     1321 cggagacgca cgcgccggct ctcatccgca cattgatgga tgtcgacctg ccggatggaa
     1381 cgcttgtcaa tttgaacttc ccgaattgcg ccgtcgattc cgttgcggga gttgaggtca
     1441 catcgcaagg caagctcgat ttcggtctca gcatcgacga gcggacggat ggacgcggct
     1501 ttccctattt ctggctgcgt ttcggcgagc gtgccggcga tttccgttcc ggcaccgaca
     1561 ttcgggcgtt gcgggataac aagatatcgg taaccccgct taaactagat atgaccgatc
     1621 atgccgttca ggatcttata gcgcgcgcat tgcggaaagg gacggtcgct tgaacgcacg
     1681 ggtttcccag caggaggggt ttgcagcgat ggtgctgcgg cttcgctcgg gcggcatcgt
     1741 caaccatgag cttctgaagg ccgtggagca gacgccccgg ctgcttttcg ctccgccgca
     1801 atatcaggac gaagtctact ccaagcggct gatcccgctc gattgcggtt ccttcatgga
     1861 aggctgcgac atggccgtcc ggcttttgca ctgtctcaac ctgaagtccg gccagcgtat
     1921 actcgaggtc ggaaccggca gcggctttac ggcggccgtt atgggccgga tcgccgagcg
     1981 cgtgctgacg atcgaccgat atcagacgct ggtcgcatcg gcgcaaaaga acctggagaa
     2041 ggcgggcatc cgcaacgtcg tcgtccggca ggcggatggt agcgcaggcg ttccgggcga
     2101 gggcacgttc gaccgcatcc tcattaccgc tgcgttcaac agccttccgc ggatgttctc
     2161 tgatcacctc gtttccggtg gtaccctgct tgtgccgatc atgatgagcg aaacgcattg
     2221 ccggatcgtt cgcgtcaacc gcaccggcag ccgtttcgac cgggaagatc tgttcgatgc
     2281 gccttacctt ccgatcgtcc cccaggtagc gtcgttcctt tagagcggct cggcgtgttc
     2341 agaacaagcg caccatacga tcttaataga atcttaactc gctccagctc cgcgcaagct
     2401 tgcagtccta ggccggacag catgatcatg tcgcgtgtgg gcattccttc cgcatcgcgc
     2461 gtcatgcgaa cggcggagac ggcatatgca ggtcgggaac agattgaacg ctgccgctgc
     2521 ggctggagac ggcttgggca atctcgccgg gcggccggcc gaccagaccg gtgccgcaag
     2581 caagagcgag agcgggacgc agcttccgcc ggcaagcttt ctcgatccaa cgccccggat
     2641 ttcgctttcc gctgatgcgc tgctttacct cggccggacc aagagaacgc tcgagaaatt
     2701 gccgcctctc acaaaagacg agtggaacaa ccatctttcg ccgcagcttg cggcgcgcga
     2761 acatcaggcg ttcggaaagt tcgcccaaac cgctgattac agggcctatt ataggggctt
     2821 catcgactac tatgacaacc ttcggccgga ggaccagaat agcctgcgct atttcggaac
     2881 gcgcgaggcg gcggtcgcgg gtttgcgctc gcttgaatat gatgtggaaa gcggcctcga
     2941 tatcggcgcc aacttcgaag gcctggtcag cgttttcctc gaagagaata agactgccct
     3001 ctcgccggca accgcgccgc cgccggccga acgcgcgtat ttcgcttggg atgcatccaa
     3061 catcagctat gagcacgagg cgccggagcc caggctgatg accgaaatag aacggctgta
     3121 ttccgagctg atctagagca cgaacgtgat tggttccaat gggggatgcg ggcggaaagg
     3181 ccgcgatatt tttctcatcc cgctccagcg tttcatcttc gcgtcctgcg tgctgcatct
     3241 ggctggaaag cgcctgtttg gacgccctat ggttagcatt tcgccaaaat cgtgccgccc
     3301 ttaaccgggc ggtaactcta atgcgcttta atcattctac agcgagttgc gttcggagtg
     3361 ggtaaagcgt catgcgtaaa tttgaatctc cccaggcagg caagtctatg atccgtctct
     3421 gcgcggctat actgcttgcg ggtgtagcga ccggttgcag ttctgacgcc agccgcttcg
     3481 gcgggctctt ctcgcggtct gacgacataa tgacaggctc gatcccgcag ggttcgagta
     3541 cgatgccgag aggcgatgtc gcaagtggcg gtgcagcgcc ctcctatgga aacggcgcgt
     3601 cggtcggtca atcctatccc gctggcggcg ggggctacaa tgcggcagcg gcgccggtct
     3661 ccagtgctcg tgtcgcatcc acgcctatgg ccgtccagcg tacgagcctc gacgagccgt
     3721 ccgccacatc cgggcagcag caggttcgga ccgcttctct cgactcgcag gccgccgccc
     3781 ttccgaggtc gcagccgcag tcggggggcg ccagggatat tccgagcaag ggggggtgga
     3841 gtgcctccaa cgcgccgacg atcttggtcc gtcagggcga cacggtgacc gttctcgcca
     3901 gacggttcgg cgtcccagag aaggagatcc tgagggcgaa cggattgaaa tcgtcaagcc
     3961 aggtagagcc gggccagcgc ctggtcattc cgacgtttgg cgccgcgggc agcgccgcga
     4021 aggcggctgc atcggggtcg atcgccgacg tggaaggtgg taagaggcgc ccgtcgccgc
     4081 tgccgactga tcagcgcgag gtcgcaatcc tccccggtca gtcccaatcg cgcgaaaaga
     4141 acgaaagccg cagcgatgtg gccgcaggca agctcaacag cgctggcgag ggcggcggca
     4201 ctggcggtta tgcggtcaag ccgggcgact cgctgaaccg gatcgccaag gcgaacggtg
     4261 tctcggtcgc tgctctgaag caggcaaacg ggcttacgac ggaagccatc cgcatcggac
     4321 aaaaactcaa tatccccagc gcttcggcca atacgccagc gaccgacgct gtcgtcacgg
     4381 cttctgtctc gcccaagaag aacgaagcca aggttgccgc aacggagcag tccaagccgg
     4441 ccgaagccaa ggcggctgcc gccaaggaga gcgtgtccga agtcgccatc aagtcggatg
     4501 tcaacgaaga tctcccgaag tcgaccggca tcgggaaata ccgctggccg gtccgtggcg
     4561 cggtcgtcgc tgcctatggc gccaatgtcg atggcaaccg gaatgacggt atcaacatat
     4621 cggttccgga gggtacgcct atcaaggcgg ctgagaacgg cgtcgtgatc tactcgggca
     4681 gcagcctcaa ggagctcggc aatgccgttc tggtgcgcca cgatgacggt accgtaacgg
     4741 tctacggcaa tgctgccgaa ctgaaggtgc aacgcggtca gaaggtccag cgcgggcaga
     4801 cgctcgcatc atcaggcatg acgggcaggg catcgcggcc gcaggtgcac ttcgaggtgc
     4861 gcaagaatgc gaccccggtc aacccggtga cctatctcga ataggtatag caggcgcgta
     4921 tcccgaaccg tacgggactt aagagcaaac acgagacgat cgaaaacgcc gcgcggctga
     4981 caggccgcgc ggcgttttcg atcgttgcag ccttgctacg gcagccttgg tggcagctac
     5041 cgctgcatcg atctgatcga agtcaagtga cctaagccgc tagcaaaatg cagtggcgga
     5101 cgcacagcgg agaggcgttc aggtgacttt tccggatggt gcaattgagg ccattgcgcg
     5161 ggcgaacggt tcggctgcca tcgcgacgcg caatgagggt ccgttcgaag ccattggcat
     5221 ccgggccgtc aatccatgaa gggtagctta gtctagcgcg gggtgaggaa aggcgggcgt
     5281 ggttctctgc ccgcatcccc gcgtgtatca ggccggcgtg tcgagctgcc tgcgcaggcg
     5341 gccggccaga tcctggatga attgccaggc gacgcggccg gacctcgatc cccgtgtcgt
     5401 cgcccattca agagcttcgg catgcagcgt ttcgggctcg accggcaatt tgaagtattg
     5461 ggcatatccg tccaccatcg ccagataatc gtcctggctg catttgtaga aacccagcca
     5521 aagaccgaag cggtcggaca gcgacacctt ttcttcgacg gcctccgagg ggttgatggc
     5581 ggtggattgc tcgttttcca tcatgttgcg gggtagcagg tgtctgcgat tggatgtcgc
     5641 atagagcagg acatttgccg gacgtccttc gacgccgccg tcgaggactg ccttcagcga
     5701 cttgtaagac gtatcgtcat ggtcgaatga gagatcgtcg cagaatacga tcaccggcac
     5761 aggcgccgcc ttgaggattt ccatgagcac gggcagagtg gctatgtcct cccggtgcac
     5821 ttcgacgagt ttgagcgcgc tgtccgcgtc atgtgcgacc tttgcatgga ctgccttgac
     5881 cagggatgat ttgcccatgc cgcgcgcgcc ccacaagagc acgttgttgg cgggatagcc
     5941 ttgggcgaag cggagggtat tgtcgaagag aatgtcgcgt acgtggtcga cgccggaaat
     6001 gagtgcgaga tcgatacggt tcggcttcgg tacgggctgg agatggcgcg tcgccggagc
     6061 ccagacgaag cagtcggcct cattccagtc gttgacggca tatgccggtc cggcgatacg
     6121 ctccagcgcc gccgaaagcc tgtgtatttc gtcgagcagg acgtcgattt tgctttcggg
     6181 catttcatac ttctccggca ggctgaactt caaaatcggc agttgacccg cgcgatgacg
     6241 ggcaagcgcc aggcttaggc aaattcttga aacgccgcga cattcgtctt ccggtcgaat
     6301 ccattgaggc tgcccggcgg cgtttttctc ggtagcacgg cggtttacgc tggaaaagac
     6361 cggcagcctg caaccaaggg gttctcctgc tgcggaattt gtgccagagg caggagaaac
     6421 ggggaagagt gcaggtgatt tgtgttgcgg cacggctttc ctatattcct gcaggtttgc
     6481 cgcgggggca agcgcgctat tgaactcaag gagtgatcga tgtttattac cgaagctttc
     6541 gcgcagacgg cggcccctgg gggcggcggc gccgatattc tgatgtccat cctgccattc
     6601 ctgctgattt tcgtggtcat gtatttcctg atcatccggc cgcagcgtgc acagatgaag
     6661 cgccgcgaag aactgctgaa gaacatccgg cgcggcgatc aggtcgtcac cggcggtggt
     6721 atcgtcggca aggtgaccaa ggtcgtcgac gataccgaac tcgaggtgga aatcgccgag
     6781 ggcactaagg tccgcgtcgt ccgcagcggc gtttccgaag ttcgcgtgaa gggcgagccc
     6841 gtcaaggaat agcccgtcgc gggactgaga agcgtgcgcg gatttttcgc ccgcatccgc
     6901 gcttgctatc ggcatggatc acggtttgat tttggatctg tctgaggcca aaatcacctg
     6961 attagggcag gggtcgcgac cggctgagac cggcggatgg agcatttcga cgtatgccca
     7021 ggttttcgcc gttcaagaac attctgattt ggctggtcgt cctggcgggc tttgcctgtg
     7081 cgctgccaaa tcttctttcc agggagcagc ttgccgattg gccgacatgg ctgccgcacc
     7141 ggcaggtacc gctcggcctc gatctcagtg gcggctctca tattgtcctg caggtcaacc
     7201 gcgaagacat cgttgcggag cgcctgcatt caacgatcga tgccatcgcc gatgcgttga
     7261 ggcaggcgga tatccactat acgggtctgt caggtagcgg gcggagcctg cagtttcgtc
     7321 tccgcgacgg aacgaaggcg cctgccgcgc gtgaggcgtt gaaggctttg tccgctcccg
     7381 ttcagcagga cggcctcttt gggaagtcca tccaggaatt ggtctacgaa gaaagcgcga
     7441 ccaacgacac gcttcggctt cgcctgaccg acgaaggcat cgacttcagg gtctccgccg
     7501 cggttgcccg ttccgtcgag gtcgtgcgcc ggcgcgtcgg cgaggtcatc gcagttgcac
     7561 catcgataga gcgtcgggaa aatgaccgcc tcagcgtgca ggtgcccggt ttggacgagc
     7621 cgcaaaggct caaagatctt cttgcccagc ggggcgacgt cgctctgagg tggctggacc
     7681 tgtcgatgcc ggcacagcag gccgtcgaca ccaggccgcc ggcagcttcc gaggtcctct
     7741 attcgctcga cgatccaccc atcccctacc tcgtggagcg acgagccttc gctgcagcac
     7801 cggacttcgt cgaggcacag ccgggcttcg accccgcaac ggccgaacct gtcgtagatg
     7861 taaggatcgc ggcgggcgca accgagcgga ttgctccgat cctgcagtcg gatccaagga
     7921 agcagtttgc gatcgtactc gacgggcagg tcatctcgac ctccgttctg gacagcgccg
     7981 tctctggtga tgccgtccgg atttccggaa atctctcgga agatggtgcc aacgatctcg
     8041 ccgctttgat ccgtgccggt ccgcttcccg tttcgctgac cgtcatcgag gaacgtactg
     8101 tcggcccgga ggacggggcc gacgcggttg caaacggcct cacggccggc ctgatcgccg
     8161 cccttgcggt cgcggcctgt atgatcggct tttacggctt cttcggcctc gtcgcagtcg
     8221 tcgccgtcat cgtcaacgtc gtactgataa tcgcggtatt gtccgtcacc ggcgccacgc
     8281 tgaccttgcc cggaattgcc gggattatcc tcacgatcgg aattgcggtc gactcgaacg
     8341 ttctcatcta cgagcgtctc agggaagagg cacgcagcag tgacaagccg ttgcgacggg
     8401 cgctggacga tggcttcgaa cgcgtcttga gaagcatcgt cgactcgaac cttacgatat
     8461 tcatcgcggg cgttatcctg ctctctctcg gctccggcgc cttcagcggg tttgcggtca
     8521 cgcttgccgt cggcagcctt accaccattc taaccgcgca cagcctgacg cgccggctta
     8581 tccgcggctg gtatcgccgt cgtcggccgc accggctgcc acggggcatt cggacgggat
     8641 ttttcagtcg ggcagacttc cgtttcatgg cgatccgtaa tccggttttc attttgatga
     8701 cagctttgtc gcttgcttcg ctggtcctcc tgacgagcct cgggctcaac atgggagcag
     8761 atttcagagg tggctccgtt atcgagctgc gggcgcgaac cggggtggct gaccgtgccg
     8821 acatccgtgc ccgcctcgac gaactgaacc tcggagaggt tcaaatcgag gaacttggtt
     8881 cggcgcgaga cgttctcatc cgtatcccct cgcaggaagc gggcgacaat gccgagcaga
     8941 cggcagttgg tctcgttcgc gcagagctcg aggatcagta tgacttccgg cgtgtcgcgg
     9001 tggtcggtcc cggcgtttcc ggggagctga cgcgggcagg atcgcttgct cttgctgctg
     9061 cggtgctgag cgttctgctt tatgtctggc ttcgtttcgg ccggcgattt gcgttaggag
     9121 ccgttgtcgc gaccttgcac gatgtgctgc tgaccctcgg attccttggg ctgacaggca
     9181 ttgaattcaa tctcgccagc attgcggcgc tgctgacgat cgtgtgctat tcgctgagcg
     9241 ataccatggt gatttatgat cgagttcggg aaaatctggt gcgctatcga aggatgccgc
     9301 tttcagtcct gatcgacacg tcgatcaatc agaccctctc gcgaaccgtt ctgacgtcct
     9361 tgacgacgat gcttgctctc gtcgcgctct atctgttcgg gggcagcgtt cttgttcaga
     9421 ccttcagtgc gacgctcata ttcggcgtac ttgtgggcac agtctcttcg atctatgtcg
     9481 ccggaccgct gctcattctc ttcaaccgcc gaaacagtcg gcttggtggc acggaggacc
     9541 cgaacaatcc ggattcgccg tcgaagacag aagcaaaggg tgccgtctga tcatggcaaa
     9601 agggatcgaa atgcgcgaag cgcactttcc gggacgggcg cctgtcgacg cttacggcaa
     9661 tggcgggttc cgcttcgccg acatgtcgca tcgcggctcc gttctgatgc tgccttccgg
     9721 tatctacgcc tggggcgtgg ccgaaggcga tctgcttgcg gtcgcgaagt tccagcgtgt
     9781 tttcgatgag gcgcccggaa tcgaggtgct gctcgtcgga accggcctgg aaatccgccc
     9841 gttgccggtc gagctgaaag cggcgcttag ggccgccggc atctcgtccg atccgatgag
     9901 cacgggtgcg gcggtgcgaa cctacaacgt gatgctggcg gaatcgcgcg ccgtcgccgc
     9961 ggcattgatc gcggtgtgag gcgttccaag gaaactcggc gtgcaacagt ccgatgacca
    10021 aattctcgcg accctgcggg acaccgaccg cgatcgttac ctggcctgtc tgctttctcc
    10081 ggtagagaag cggcattcgc tcgcggcact ttatgccttc tacgccgaga tcgcccgcgt
    10141 ccgtgacatg gtcaaggagc cgctacccgg tgagatccgg ctgcagtggt ggcgcgacat
    10201 tctcgaaaac gaaaagtcgg acggcggggg gcatccttta gccgaagcgc tcctgcgatg
    10261 cgttcgcgag catcagctgc ctgttcgcgt tctgcaggac atgatcgacg cacgcatttt
    10321 cgatctttac gaggatccga tgcaggacag ggcggcgctc gaaggctatg ccggtgaaac
    10381 cgcatcggcg ctcatccagc tctcttcgct gatcctcgac ccggtcaacg cggcggaatc
    10441 cgcagacgcg gccggtcatg ccggtgtcgc tcagacgatc gccggtctcc tgcttcttct
    10501 gcctgttcat gtcgggcgtg gccaggtcta tttgcctgct gagattttga gcgcaacggg
    10561 gctcgacagg gaggctctcc ttgcggtgaa cgacgagggc gccgtcgaaa acgccatccg
    10621 cgcattttgc ggtctcggcc gcgatcatct tgccagggcg cgcaagagtg ccagttcgat
    10681 ttcggcccgg aatttctccg ccttcatacc cgtcgccctg gccgaacctg tgttcgaccg
    10741 tgccgaggcc gccggagcgc gaatccttcg tcagccactg caggcaccgc aatggcagcg
    10801 gcaatggcgg atgtggcggg cgagccggcg tcggaaattt tagcggatag ccgttcggcc
    10861 gtcatcctgg cgcaagtctc ccattgttct atcggattcc cgtcgctctc aagggaccca
    10921 cgcgatgctt tggttactgt tcccggctgc gattgccgcc gtctgcgtgt tttatatcag
    10981 gatgaacgca cgcgccgagc gcgatgcaga caatccggac gcgggttcgg cgatcgtgga
    11041 attcggccgg gcatttcccg acgaggccat ccgggcgctg cattcaagcg ttgacggaac
    11101 agcgatcttc ctgcgtctcc atgacggtaa ggcgggcttc atacagtcgc atggcacgca
    11161 ttatgtctgt catgtcgttc aaccggacaa ggtgcgggtc aatacatcag acagtgggcg
    11221 cgggttgatc gtccacttcc cggatttcgc ctatctcgac aatgctttcg aatttcggac
    11281 cgcggccgag gcggccgagg tctcgctatg gctgctcggc agtttcagga cgaaactcga
    11341 gaaggaagcg tcgggcaacg cggcgcctgg accggattga ccgttgacgt caacgggtcg
    11401 ctgccccgcc gatcaataga tttttgtcga gccaggttgc acagtcgctc agcgctcgtg
    11461 aggccatcgc ctgcttcttg gcgcttgcct tttccttgcc gcgaaagcgc ttcgccccct
    11521 ccggccttgt gagggccccg ggatcgagcg gcggaaacag cccgaaattg acattcatcg
    11581 gctggaagga gcgcttgccc ggttcatcgt cggagacgat gtgccctccg gtgatgtgat
    11641 tgaggagagc gccgaaggcg gtcgtcactg gcggaatggc gggagaggcg cccttgcgtt
    11701 ccgccgcggc gaagcggccg gccagaagtc cgatactcgc gctttctaca tagccctcgc
    11761 agccggtgat ctggccggcg aagcgcagtc ccgcacgcga tttgagggtc agcgactggt
    11821 cgagcagggt cggcgaattg atataggtat tgcggtgcag accgccgagt cttgcaaact
    11881 ctgccttctc gagccccggg atcatccgga agacctctcc ttgcgcgcca tatttcagtt
    11941 tcgtttgaaa gccgaccatg ttgtagagcg tgccgagcgc attgtcctgc cgaagctgta
    12001 cgaccgcata gggcttgacc gacggattat gggcgttggt aagccccatc ggcttcattg
    12061 gcccgtgacg cagggtctca cgtccgcgtt ctgccatcac ttcgattgga aggcacccgt
    12121 cgaaataggg tgtgccttcc cattccttga agccggtctt gtcgccggcg atgagcgcat
    12181 cgacgaaggc gttgtattcc tcctgcttca gcggacagtt gatgtaatcc ttgcccgtgc
    12241 cgccggggcc taccttgtcg tagcgcgact gatgccagca gatatccatg tctatcgtat
    12301 ccgcatggac gatcggcgcg atggcgtcga aaaaggcgag ggcgtcagcg ccggtttccg
    12361 cccggatcgc gtcggcgaga tccggtgccg taagcggacc ggtcgcaatg atggcgagat
    12421 cccattcctt cggcggcagg ccgcggattt cctcccgcag gacggtgatc agcggatggg
    12481 ccgcgatcgc cgcgctgaca gcctcggcga agccttcgcg atcaacggcc agcgcgccgc
    12541 cggcaggaac ctgattgaga tcggcgcatc gcatgataat cgaccccgcg aggcgcatct
    12601 ccgcatggag aacgccgacg gcattgctgg tcgcatcgtc ggaacggaaa gaattggagc
    12661 agacgagttc ggcaaggctg tcggtcttgt gagcgtccgt gccgcgcacg ccgcgcatct
    12721 catgcaggat gaccgggacg cccgcttccg cgatctgcca ggcggcttcc gatccggcaa
    12781 ggccgccgcc aatgacgtgg acgggggaat aggaggatgt gagtttgttc atgcgcggct
    12841 tcctaccaca gccgatcttc gcttccaaga aacgaaaacg gcgcccgagg gcgccgtttt
    12901 caagctgtgc aatgctcgtc cagttaacgg aaggagcggg ccgcgatgtt gcggatatcg
    12961 tagcgagtga tgccgatatc gttcagggcg tggttcgaaa ggctgccgag ttcggcaacg
    13021 gtgcggcggt agttgatcca gctctttgca atgcgaatcg ggttcatggt cgtttcctca
    13081 agaagatcgc tcgcgacagt gattgtctgc gtcgttgagg ttcttatacg cctgtctaat
    13141 tgggagatgg agtgcaaagt agatgcatct gctatgcatt tgtgcagtgc atcgatcatt
    13201 tgatgagcat gaatacagcc tcggctgaaa atgcgccatc gcttgagatt caaactcccg
    13261 gggtcactgc atgagaccaa aaagcagcag ataaagaaaa acgcccgccg gcagagccgg
    13321 aaggcgtttg aaaggccaag atctggcgaa aagctattct gcttacttga ttgcctgacg
    13381 cgcaacgtag ggaatgtctg cccggccgat gccgaggtca gtcagttccc gatcgctcat
    13441 gcggccgagt tcgttgcagg tctgacgata cttgcgccag ttgttgaaag aacgtgcgag
    13501 gttcatgatc ctattccttt ctaggtcgtt cgccagcgct ctatctctct tgctggccat
    13561 catctgatga cccggaatat aagcgatttg acccaaaatt acagagataa tgctgcatcg
    13621 cacccatgca tgtcttgcaa agctttgtcc gatattaatt ccgacttaac ctttttggtg
    13681 ccggtccgcc ttccaaattt ttgcagcgag cttttgcgcg cttctgagag gcgccgcgtt
    13741 tagcgcagat gcgcggtgta gctgtaatgg aataagaaag cagaaataaa caacgcccgc
    13801 tgggggagga ggtccagcag gcgtcgcttg ttctgattgg caactgggag gaggagtgct
    13861 gccaatccgt atcgggtgcg ctgggaggag gagtgcgctg cccgatcgat gagtggaata
    13921 taggcagcat acccgtagct tggtaggggt ctctccgaat ggcacctatg cgaacagtgc
    13981 atagctaagg agccggaaag tgaacatgtg ccaaaatagt aggcaggtgg atcacttgaa
    14041 gagtgcagtg cgggagagga agagaacgcc cgccggggga ggaccggcgg gcgtcacaaa
    14101 ggcgattggc aaccgggagg aggagtgctg ccaatcctat caaagcggcg ctgggaggag
    14161 gagtgcgctg cttcgaattc catgaggtga atataggacg cttaaatcga tttgtgcagt
    14221 gcaatgtcca catggaagat atgcagtttg cgcaacgcat cggcatgcgc cgccgcatcg
    14281 cgtttctgtc gtcacccggc tgtaaagagg gcaatcctcc cgttcggaga caagccggat
    14341 aagggcaacg ggcgcgagca ttttcccttt gatccgatga aaacgagtga tatagagacg
    14401 ccgcgctcgg gaagcccctg aacctttcgc acggcccaag aaatatctgc ggcattccgc
    14461 tggtgagatc ttgtgaggaa tgcgattggg cggtgcgatc gtctgaatgc gggtatggtg
    14521 aaattggtag acacgccaga tttaggttct ggtgccgcaa ggcgtgggag ttcaagtctc
    14581 tctacccgca ccaggacgat atagcagtca cgcgtcgttt tcggcgctga ggaaggccgg
    14641 caaggcgcca ttttgcttgc aaagcggtcg gaattgcgta aagccactcc cggcaaatgg
    14701 atcggctcac gtgctcgcct agccttatca agggaagagc accgtcaacg tgaagtgaag
    14761 gtttgaacat gcaggttatc gaaacgctcg ctcaagggct gaagcgcgaa ctcaaggtcg
    14821 tcattccggc cgacgaaatg gaagctcgga tgaacgagcg tctcgtcgag gtgaaggatc
    14881 gtgttcgcat caatggcttc cgccccggca aggtgccggt tgcgcatctg aagaaggttt
    14941 acggcaagtc gatcatggcc gatctcgtca acgagatcgt tcgtgaaaag ccgacggaaa
    15001 tcctcaccag ccgcggcgag aagtctgcga cccagccgga aatcgggatg accgaggacg
    15061 aggcggaagc tgacaagatc ctgaaggccg aagcggattt cgaattcacc gtcgcctacg
    15121 aaatcattcc gccgatcgaa ctcaaggacg cgagcggcat caaggtcact cgcgaagtcg
    15181 ttgatgtcgg cgaagacgaa gtcaacgagc agatcgagcg catcgctgaa agcgcccgta
    15241 cttatgaatc gaagaagggc aaggccgcca atggtgaccg cgtcacgatc gactacctgg
    15301 gcaaggtcga cggcgaagcc tttgacggcg gcaaggacga ggatgcggaa ctcgtgctcg
    15361 gctccaaccg cttcatcccc ggcttcgaag agcagctcgt tggcgcgaag gccggcgatg
    15421 aaaagaccat cacggtcacg ttcccggccg actacccggc agccaatctc gccggcaagg
    15481 aagcaacctt cgacgtcacc gtgaaggacg tcgctgccgc ggctccgatc gagatcaatg
    15541 atgagctggc aacgaagctc ggcctcgaat ccgtcgacaa gctgaaggaa atcgttcgcg
    15601 gccagatcga aagccagttc ggctcgataa cccgccagaa ggtcaagcgc cagcttctag
    15661 accagctcga cgaactctac cagttcgata cgcccgagcg ccttgtcgac gccgaattcg
    15721 agaacatctg gcgccagatc aacaccgatc tgcagcaggc cggcaaaacc tttgccgatg
    15781 aagacacgac ggaagaggaa gcccgcgccg aataccgcaa gcttgccgag cgtcgcgtcc
    15841 gccttggact ggttctctcc gaaataggcg agaaggccgg tgtccaggtc agcgatgacg
    15901 agatgcagaa ttcactgttc cagcagctgc gtcagttccc cggccaggaa aaggaaatca
    15961 tcgaatattt ccgcaatacg cccggcgctg ccgcctctct gcgcgcaccg ctcttcgagg
    16021 agaaggtcgt cgaccatctg ctcaccgagg tctcggtgac cgacaagaag gtgtccaagg
    16081 aagagctgac cgcggaagac gacgccgacg aaaaaccggc caagaagacg gcttccaaga
    16141 agaaggcagc tgccaaggcc gatgccgccg agggcgaaga agccgcagcg ccgaaaagga
    16201 aggctccggc caagaaaaag gcttcggacg agagcgccga gtaaatcgag tgtcttgcaa
    16261 tgaattgaaa aagccgccct ccgggcggct ttttctatgc gctgatgccg ggcgccaacc
    16321 ctgacgttcg cccaaccctg acgttcgccg cgcttgctgg gagctctcgc gccgtcgacg
    16381 cgctgaactg atgtggagcg aacgcccctc atccgcctgc cggcaccttc tccccgcgag
    16441 cggggggaag ggacaagcgg catgctcctg ttgcttccga gccgttgcgg agtattgagc
    16501 ggttgcggca agtcccttct cccgatggga gaaggtgccg gcaggcggat gaggggccga
    16561 acgcgcctgg tgtcctctac gccaagagac gatcgtcgag ccggacgcct gaccagcgcc
    16621 cggtccgatc atctcattca gcagcttccg catagctttc gatcggcggg caggtgcaga
    16681 cgagattgcg gtcgccatag acattgtcga cgcggttgac cggcgaccaa tatttgtcga
    16741 cgcggaatgc gccgggcggg aagcaggctt gctcgcgcga atagggccgg tcccagtcgc
    16801 cgacgaggtc ctcgaccgta tgcggggcgt tcttcagcgg attgttgatc ttgtccatcc
    16861 ggccttccgc tatggcgcgg gcttcctctc ggatcgccag caaggcatcg cagaagcggt
    16921 cgagctcggc cttggtctcc gattccgtcg gctctatcat caaggttccg gcaaccggcc
    16981 agctcatcgt cggcgcatgg aaaccgcagt cgaccagccg tttggcaacg tcgtcaacgg
    17041 tcacaccggc gctctcggca agggggcggg tatcgatgat gcattcatgc gcaacgcggc
    17101 ctttggccga cttgtagagc acgtcaaagg cacccttcag ccgggcggcg acataattgg
    17161 cattgaggat cgccaccttg gtcgcttgcg tcaggccttc gccgcccatc atcaggcaat
    17221 agctccagga gataggcagg atcgaagccg acccgaacgg tgcggcggaa acggcgccct
    17281 catgttcgcc gctttccgga tgccccggaa ggaagggtgc gaggtgggct ttgaccccga
    17341 tcggacccat gccgggcccg ccgccgccat gcggaatgca gaaggtcttg tgcaggttca
    17401 agtggctgac atccgagccg atatcgccgg gacgggaaag gccgaccatc gcattcatgt
    17461 tggcgccgtc caggtagacc tggccgccat gcttatggac gatctcgcag acctcgcgca
    17521 cgttctcctc gaatacgccg tgggtcgagg gataggtgat catgcagcag gagagcgttt
    17581 ccgcatattg ctcggctttg gcgcggaagt cgtccatggc gatctcgccg gcgtcgctca
    17641 ccttgacgac caccaccttc atgccggcca tctgcgccga ggcgggattg gtgccgtgcg
    17701 ccgaagtcgg gatcaggcag acgtcacgat gttcgttgcc attggcaata tggtaggcgc
    17761 ggatagcgag cagacctgca tattcgccct gggcgcccga gttcggctgc atggagatcg
    17821 cgtcgtagcc ggtgatcgcg cagagtttct gcgacaggtc ttcgatcaga tggtgatatc
    17881 ccatcgcctg gtcggcgggc acgaacgggt ggatctccga gaattccggc caggttattg
    17941 gcagcatctc cgccgtcgcg ttgagcttca tcgtgcagga gccgagcggg atcattgccc
    18001 gatcgagcgc aagatcgcga tcggcgaggc gtcgcatgta acgcgtcatc tcgctttcag
    18061 cccggttcat gtggaagatc ggatgggtga gatagtcgct ggtgcgcagc aattccttcg
    18121 gcaggcggta gtccggctcg aactgatcga cgctgaaatc gccgccgaaa gcccgccaga
    18181 cggcctcgag ggtgattggc cgggagcgct cgtcgagact gatgccgatc ttcgtcgtcc
    18241 cgatcttgcg aaggttcacg tcctcggcga cggcagcctt gaggatgatg ccttgcagtt
    18301 tgccgacctc gacggtgatc gtatcgaaga agacgtccgg ctcgacggta tagccgagtt
    18361 tttcgaggcc gagcgcaagg cggaccgtct tctggtggac gctctgggcg atcgccttga
    18421 tgccctcggg cccgtggaag acggcataca tggaggccat gacggcaagc agcacctgcg
    18481 cggtacagat gttggatgtc gccttttcgc gccgtatatg ctgctcccgc gtctgcagcg
    18541 aaagccgata ggcgcggttt ccgcgcgcgt cgaccgacac gcccacgaga cggccgggca
    18601 tcgagcgctt gtaggcgtcc ctgaccgcca tgtaggcggc gtgcggcccg ccatagccca
    18661 ccggaacgcc gaagcgctgg gttgagccga cggcgatgtc tgcgcccatc tcacccggcg
    18721 acttcaaaag cgccagcgcc agcggatcgg ctgcgacggc ggcgattgcg cccagttcgt
    18781 ggagcctggc gatcaggccc gagaaatcgc gaacatgacc ataagtgccg ggatactgga
    18841 aaatcgcgcc gaaaacgatt gcggcgtcca gatcctcgaa gggattgccg acgacgatct
    18901 gccagccgag cggctctgcc cgggtcttca acagtgcgat cgtctggggg tggcagttct
    18961 cgtcgacgaa aaaggccttc gctttggact ttgcgacgcg ctccgcaatg gccatcgctt
    19021 ccgccgccgc cgtcgcctcg tccagcagcg aggcgtttgc gacgtcaagt ccggtcaggt
    19081 cgcaaaccat cgtctgaaaa ttgagcaggg cttcgagacg cccctggctg atctccggct
    19141 gatagggcgt ataggcggtg taccaggcgg gattctcgag aatattacgc tggatgaccg
    19201 ggggcgtgat ggttccgtaa tagccctggc cgatcagaga aacgaccttc cggttgcggt
    19261 tcgccgtctc gcgcagcttg tcgagcgcct cgcgttccgt catcggcgca ccccaggcga
    19321 gtggagtctg ctgccggatc gacggcggaa cggtgtcgtc gatgagcgca tcgaggctcg
    19381 gatagccgac gaccttcagc atttcgtcca tttcggccgg cgaggggccg atgtgacggc
    19441 gattggcgaa gtcgtagggc ttgtagtcgg taaaggtaaa gtccttgggc atgctcatca
    19501 ggcgacgagc tccttatagg ccgcttcgtc caggagcgcc ttggcggcgt ccggatcggc
    19561 gagtttcagc ttgaagaacc aggcagcgcc ttgcggatcg ctgttgacga gtgaggggtc
    19621 gtcgacgatc gcctggttgg cctcgacgat ttcgccgtca agcggacaat agacgtccga
    19681 tgccgccttg accgactcga cggtcgctgc cgtatcgccc ttggagaagg tgctcccggc
    19741 ttccggcagt tccacgaaga caagatcgcc gagctggccc gctgcatgct cggtgatgcc
    19801 gacggtcgcg acatcgccat cgatcttcag ccactcatgt tcctcggtga atttaagcat
    19861 atgcaggttc tcctgatcag cgtttatacg tttgtttgat gaagggcagg gcggagacgg
    19921 cgacgggcag aaacttgccg cgcacctccg caaacagtct ggtaccggtt tccgcgtgtt
    19981 cggcgtcgac atagcccatg gcaatcggtc catcgacact cgggccaaaa ccgccggagg
    20041 tgaccgttcc tgctgccgtg ttgccttccg catcggcaaa tagccgcacg ccacctcgaa
    20101 cgggcgcacg gccctcgggc ttcaagccga cacgccgcct ggaaaccccg tgggtgaact
    20161 cggccagaat tctgtcggcg ccgggaaagc cgcctgcgcg ctcaccgtcg gcgcggcggc
    20221 ttttctggat tgcccattcg agtcccgctt cgatagggct ggtgctcgtg tcgatatcgt
    20281 tgccgtagag acaaaggccc gcttcgaggc ggagcgagtc gcgcgcgccg aggccgatcg
    20341 gcaggacgtc ggggtgttcg agaagacgtt gagtgacatc aacggccgct tcggtgggaa
    20401 tggaaatctc gaagccatcc tcgccggtgt aaccggagcg ggatatgatg cagcttacgt
    20461 cgtgaaggtc ggcttcggcg acatccatga agcgcatcga cgcgacgtct gcccacagtt
    20521 cgcagagaac cgcccccgcc cgagggcctt gaagggcaat cagggcgcgg tcggtcaaca
    20581 tggtcacgtc gcagagactg ccaagaccat tcttgagatg cgcgaaatcg gcgtccttgc
    20641 aggctgcatt aacgacgagg aaaagatgat cgccgcgatt gacgatcatc aggtcgtcga
    20701 gaatgccgcc tgtcgcattg gtgaacaggc catagcgttg ccggccttcc gcgaggctga
    20761 gcacgtccac cggcacgagc ttttccagag ccagtgccgc gtcctcgatc tttccggact
    20821 tcggccgcac gaggatctga ccc
//
