LOCUS       NZ_JAJNDF010000037      3570 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Rhodococcus qingshengii strain IEGM 746
            NODE_37_length_3570_cov_360.325747, whole genome shotgun sequence.
ACCESSION   NZ_JAJNDF010000037
VERSION     NZ_JAJNDF010000037.1
KEYWORDS    WGS; RefSeq.
SOURCE      Rhodococcus qingshengii
  ORGANISM  Rhodococcus qingshengii
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Rhodococcus; Rhodococcus erythropolis group.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Elkin,A.
  TITLE     Development of a sustainable strategy for remediation of
            hydrocarbon-contaminated territories based on the waste exchange
            concept
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Elkin,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2021) LAM, Institute of Ecology and Genetics of
            Microorganisms, Ural Branch of the Russian Academy of Sciences,
            Golev Str., 13, Perm, Perm Kray 614081, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-NOV-2021
            Assembly Method                   :: SPAdes v. 3.14.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 275x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021026075.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 04:42:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,393
            CDSs (total)                      :: 6,335
            Genes (coding)                    :: 6,273
            CDSs (with protein)               :: 6,273
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 21 of 62
            Pseudo Genes (incomplete)         :: 52 of 62
            Pseudo Genes (internal stop)      :: 5 of 62
            Pseudo Genes (multiple problems)  :: 14 of 62
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:48:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3570
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAJNDF010000037.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..3568)
                     /NRPS_PKS="Domain: AMP-binding (65-473). E-value: 2e-101.
                     Score: 331.5. Matches aSDomain:
                     nrpspksdomains_LQ424_RS31560_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (579-647). E-value: 3.8e-23. Score:
                     73.5. Matches aSDomain: nrpspksdomains_LQ424_RS31560_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (663-965). E-value:
                     4.7e-115. Score: 375.6. Matches aSDomain:
                     nrpspksdomains_LQ424_RS31560_Condensation_LCL.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211568.1"
                     /locus_tag="LQ424_RS31560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_31560"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_230793650.1"
                     /sec_met_domain="AMP-binding (E-value: 5e-113, bitscore:
                     363.8, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.5e-16, bitscore:
                     46.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 7.9e-67, bitscore:
                     211.5, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLFDASTVASFAERFERILTAVAADSAVRVGDVELLDSVERSLVL
                     DGWNDQVRVKPEVDTTLVSMFDAQVLARGMSTAVVFEDRRVSYVDLDAAANRLARKLIE
                     SGVGPESLVAVLLPRSVDLVVSLLAVLKAGGAYLPIDPSYPAERIAFTLDAARPVCVLG
                     GAELDLDIPVFDVASMDVSKYDGSSITDHDRVVPLRDSNSAYVIYTSGSTGRPKGVAVS
                     HRNVVELFANSQSRFEFDDSDVWTLFHSYAFDFSVWELWGALSFGGTVVVVDFLTSRSP
                     EQFRELVARERVTVLNQTPSAFYQFAEADRVAGQAATPLSLRYVIFGGEALDFAQLERW
                     FERHDDRSPRLVNMYGITETTVHVSFLELDAVWAREATASVIGRGLGGLSVFVLDDRLR
                     PAPVGVRGEMYIAGEQLSRGYVGQPGLTAGRFVANPFAHKSSGAVMYRTGDVGRWSVDG
                     QLEYLGRSDFQVQLRGFRIELGEIEAALLRVQGVARAVVLVRSDAALGDRLVGYVVPES
                     GVDLVVADVLSSVAQFLTGYMVPDSVMVLEELPLTVNGKLDRKLLPEPVFEKAVFRAPV
                     TAVEEIVASVFAEALGVDRVGLDDDFFALGGNSLIATRVAARLGQALDAQVPVRVLFEA
                     SSVELLAARVESEVGSGARAALTAMVRPERVPLSLAQQRMWFLNRFEPGSAVNNIPVAI
                     RLSGSLDVAALQAAVSDVVARHEVLRTVYPEVDGVGYQQIVSVDRVELDLSPVAVSESG
                     VVAAVSEFLSAGFDVAVEVPVRARLFAVSESEFVLAMAVHHISGDGFSMGPLTRDVMVA
                     YEARSRGEVPGWAPLAVQYADYALWQREVLGSEDDAASLISRQVEFWSRTLAGLPDQLV
                     LPWDRPRPAVQSFAGGTVGVSVTPEVHRALMELVQRTNTTMFMVVHAALAVTLARLSGS
                     DDIVIGSPIAGRGDEILDDLVGMFVNTLAFRTRVDPGESFSALLARSREADLAAFAHAD
                     VPFERLVEVLNPVRSTAYHPLFQVGLSFQNLAQSTFELSGLTLSGVEYESGNSQFDLHL
                     IVTDHHDEVGAAAGITGMITYASALFDESTVAGFVDQFVRVLETISAEPSVAVGDIILL
                     GEVDRALVLESGCGVCRAGVSGTLVDLFDEQVVRGADRVALVCGGVELSYGEFDSRVNR
                     FARYLISVGVGPESLVALAMRRSV"
     source          1..3570
                     /collected_by="M.I. Rychkova"
                     /collection_date="2001"
                     /db_xref="taxon:334542"
                     /geo_loc_name="Russia: Udmurt Republic"
                     /isolation_source="oil-polluted soil, oil-extracting
                     enterprise"
                     /mol_type="genomic DNA"
                     /organism="Rhodococcus qingshengii"
                     /strain="IEGM 746"
                     /submitter_seqid="NODE_37_length_3570_cov_360.325747"
     gene            complement(<1..>3570)
                     /locus_tag="LQ424_RS31560"
                     /old_locus_tag="LQ424_31560"
     region          1..3570
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..3570
                     /SMILES="NC(C(O)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..3570
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:3568](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..3568)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(520..636)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ424_RS31560_0010"
                     /evalue="5.10E-19"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="1016"
                     /protein_start="977"
                     /score="60.7"
                     /tool="antismash"
                     /translation="READLAAFAHADVPFERLVEVLNPVRSTAYHPLFQVGLS"
     aSDomain        complement(673..1578)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ424_RS31560_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.70E-115"
                     /label="LQ424_RS31560_Condensation_LCL.1"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="965"
                     /protein_start="663"
                     /score="375.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHISGDG"
                     /tool="antismash"
                     /translation="ERVPLSLAQQRMWFLNRFEPGSAVNNIPVAIRLSGSLDVAALQAA
                     VSDVVARHEVLRTVYPEVDGVGYQQIVSVDRVELDLSPVAVSESGVVAAVSEFLSAGFD
                     VAVEVPVRARLFAVSESEFVLAMAVHHISGDGFSMGPLTRDVMVAYEARSRGEVPGWAP
                     LAVQYADYALWQREVLGSEDDAASLISRQVEFWSRTLAGLPDQLVLPWDRPRPAVQSFA
                     GGTVGVSVTPEVHRALMELVQRTNTTMFMVVHAALAVTLARLSGSDDIVIGSPIAGRGD
                     EILDDLVGMFVNTLAFRTRVD"
     CDS_motif       complement(685..774)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ424_RS31560_0009"
                     /evalue="7.30E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="961"
                     /protein_start="931"
                     /score="44.1"
                     /tool="antismash"
                     /translation="DIVIGSPIAGRGDEILDDLVGMFVNTLAFR"
     CDS_motif       complement(1042..1080)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ424_RS31560_0008"
                     /evalue="2.30E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="842"
                     /protein_start="829"
                     /score="19.9"
                     /tool="antismash"
                     /translation="QYADYALWQREVL"
     CDS_motif       complement(1129..1194)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ424_RS31560_0007"
                     /evalue="9.40E-07"
                     /label="C3_LCL_132-143"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="813"
                     /protein_start="791"
                     /score="21.5"
                     /tool="antismash"
                     /translation="VHHISGDGFSMGPLTRDVMVAY"
     CDS_motif       complement(1396..1509)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ424_RS31560_0006"
                     /evalue="1.10E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="724"
                     /protein_start="686"
                     /score="56.4"
                     /tool="antismash"
                     /translation="VNNIPVAIRLSGSLDVAALQAAVSDVVARHEVLRTVYP"
     CDS_motif       complement(1531..1563)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ424_RS31560_0005"
                     /evalue="4.70E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="679"
                     /protein_start="668"
                     /score="9.6"
                     /tool="antismash"
                     /translation="SLAQQRMWFLN"
     aSDomain        complement(1627..1830)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ424_RS31560_PCP.1"
                     /evalue="3.80E-23"
                     /label="LQ424_RS31560_PCP.1"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="647"
                     /protein_start="579"
                     /score="73.5"
                     /tool="antismash"
                     /translation="EEIVASVFAEALGVDRVGLDDDFFALGGNSLIATRVAARLGQALD
                     AQVPVRVLFEASSVELLAARVES"
     aSModule        complement(1627..3372)
                     /complete
                     /domains="nrpspksdomains_LQ424_RS31560_AMP-binding.1"
                     /domains="nrpspksdomains_LQ424_RS31560_PCP.1"
                     /locus_tags="LQ424_RS31560"
                     /monomer_pairings="Thr -> Thr"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(2119..2184)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ424_RS31560_0004"
                     /evalue="3.60E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="483"
                     /protein_start="461"
                     /score="35.0"
                     /tool="antismash"
                     /translation="YLGRSDFQVQLRGFRIELGEIE"
     aSDomain        complement(2149..3372)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ424_RS31560_AMP-binding.1"
                     /evalue="2.00E-101"
                     /label="LQ424_RS31560_AMP-binding.1"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="473"
                     /protein_start="65"
                     /score="331.5"
                     /specificity="substrate consensus: Thr"
                     /tool="antismash"
                     /translation="FDAQVLARGMSTAVVFEDRRVSYVDLDAAANRLARKLIESGVGPE
                     SLVAVLLPRSVDLVVSLLAVLKAGGAYLPIDPSYPAERIAFTLDAARPVCVLGGAELDL
                     DIPVFDVASMDVSKYDGSSITDHDRVVPLRDSNSAYVIYTSGSTGRPKGVAVSHRNVVE
                     LFANSQSRFEFDDSDVWTLFHSYAFDFSVWELWGALSFGGTVVVVDFLTSRSPEQFREL
                     VARERVTVLNQTPSAFYQFAEADRVAGQAATPLSLRYVIFGGEALDFAQLERWFERHDD
                     RSPRLVNMYGITETTVHVSFLELDAVWAREATASVIGRGLGGLSVFVLDDRLRPAPVGV
                     RGEMYIAGEQLSRGYVGQPGLTAGRFVANPFAHKSSGAVMYRTGDVGRWSVDGQLEYLG
                     RSDFQVQLR"
     CDS_motif       complement(2275..2364)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ424_RS31560_0003"
                     /evalue="2.40E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="431"
                     /protein_start="401"
                     /score="42.2"
                     /tool="antismash"
                     /translation="PVGVRGEMYIAGEQLSRGYVGQPGLTAGRF"
     CDS_motif       complement(2899..2958)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ424_RS31560_0002"
                     /evalue="6.10E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="223"
                     /protein_start="203"
                     /score="34.0"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVAVSH"
     CDS_motif       complement(3145..3186)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ424_RS31560_0001"
                     /evalue="9.70E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="LQ424_RS31560"
                     /protein_end="141"
                     /protein_start="127"
                     /score="18.7"
                     /tool="antismash"
                     /translation="LAVLKAGGAYLPID"
ORIGIN
        1 accgaccggc gcatcgccaa cgccaccaac gactccggcc ccacacccac cgaaatcaaa
       61 taccgcgcaa aacgattcac ccgcgaatcg aactcaccat acgacagctc gacaccacca
      121 cacaccaacg cgacacgatc cgcaccacga accacctgct cgtcgaacaa atccaccaac
      181 gtccccgaaa caccagcacg acacaccccg caaccggact ccaagacgag cgcgcggtcg
      241 acctcaccca acaagatgat atcgcccaca gcgacactcg gttccgctga aatggtttcg
      301 agaacgcgca cgaactgatc cacaaaaccg gccaccgtcg attcgtcgaa caatgccgac
      361 gcataggtga tcatgccggt tatcccagcc gcggcgccca cctcatcgtg atgatcggtc
      421 acgatcaagt gaaggtcgaa ctgagagttc ccactctcgt actcgacacc ggaaagcgtc
      481 aaaccactca attcgaaagt cgactgcgcc aagttctgga acgacaaccc cacctggaac
      541 aacggatgat acgccgtcga ccgaaccgga ttgagcacct ccaccaaccg ctcgaacggc
      601 acatccgcat gcgcaaacgc cgccaaatcc gcctcacgcg aacgagcaag caaagcgctg
      661 aacgattccc ctggatcgac tctggtgcgg aatgccaacg tgttgacgaa catcccgacc
      721 agatcatcaa ggatctcgtc cccacgtccc gcaatcgggg aaccgatcac aatgtcatcg
      781 ctaccggaca aacgagccaa cgtcaccgcc aacgccgcat gcaccaccat gaacatggtc
      841 gtgttcgtac gctgaaccaa ctccatcaac gcccgatgaa cctccggagt caccgacaca
      901 ccaactgtgc cgccggcgaa cgactgcacc gcaggcctcg gccgatccca gggcaacacc
      961 aactgatccg gcaaccccgc caacgtccga gaccagaact cgacctgacg cgaaatcaac
     1021 gacgccgcat catcttccga acccaacacc tcacgctgcc acaacgcgta atccgcatac
     1081 tgcaccgcca acggcgccca ccccggaacc tcaccccgag aacgagcctc gtacgcaacc
     1141 atcacatcac gcgtgagcgg ccccatcgag aacccgtcac cactgatgtg atgaaccgcc
     1201 atcgccaaca cgaactccga ctccgacacc gcgaacaacc gcgcccgaac cggaacctcc
     1261 accgccacat cgaaaccggc agacaagaac tccgacaccg cagcaacaac accggactcc
     1321 gacaccgcaa ccggagacaa atccagctct acccgatcga ccgagacgat ttgctggtat
     1381 ccgacgccgt cgacctccgg atacaccgtc cgcaacacct catgccgcgc aacaacatcc
     1441 gacacagcag cctgcaaagc cgccacatcc aacgaccccg acaaccgaat cgccaccgga
     1501 atattgttca ccgcagaccc cggctcgaac cggttcaaga accacatccg ctgctgcgcc
     1561 aacgacaacg gcacccgctc cggacgcacc atcgccgtca acgccgcccg agcacccgaa
     1621 ccgacctccg actccacccg cgcagccaac aactccaccg acgacgcctc gaacaacacc
     1681 cgcaccggaa cctgcgcatc gagcgcctga cccaaccgag ccgccacccg cgtcgcaatc
     1741 agcgaattac cacccaaagc aaagaaatcg tcatcgagac ccacccgatc cacacccaac
     1801 gcctcggcaa agaccgacgc aacgatctcc tccaccgccg tcaccggagc ccggaacaca
     1861 gccttctcga acaccggctc cggcaacaac ttccgatcca acttgccatt gactgtcagc
     1921 ggcaattctt cgagcaccat cacagaatcc ggcaccatgt acccggtcag gaactgcgcc
     1981 accgatgaca agacatcagc aaccaccagg tcgacaccgg actccggcac cacgtatccc
     2041 acgagccgat ctccaagggc agcatccgaa cgcacaagca cgaccgcacg cgccacacct
     2101 tgaacacgca acaacgcagc ttcgatctca ccgagctcga tccggaatcc acgcaactgg
     2161 acctggaaat cactgcggcc taaatactcg agttgcccgt cgacactcca ccgcccaacg
     2221 tcaccggtgc gatacatcac cgcaccggaa ctcttgtgcg caaaaggatt cgccacaaag
     2281 cggccagccg tcagacctgg ctgtccgaca tacccacgcg aaagctgctc tcccgcgata
     2341 tacatctcgc cgcgaactcc caccggagcc ggacgcaatc gatcatcgag cacgaacacc
     2401 gagagtccac ccaacccgcg tccgatcaca gacgctgtcg cctcacgcgc ccatacagca
     2461 tccagctcga ggaacgacac gtgaacagtc gtctccgtga tgccgtacat gttgaccaac
     2521 cgcggggacc gatcgtcgtg gcgctcgaac caccgttcca actgcgcaaa gtccagcgcc
     2581 tcgccaccga atatcacgta ccgcaacgac agcggtgtcg ccgcttgacc cgctactcgg
     2641 tcggcctcag caaattgata gaacgcggat ggagtctgat tgagtaccgt cactcgctcg
     2701 cgagcaacca actcccgaaa ctgttcgggc gaacgcgacg tgaggaaatc gaccaccaca
     2761 accgtgccac caaatgacaa cgccccccac aattcccaca ccgagaaatc gaatgcgtaa
     2821 ctgtggaaca acgtccatac gtcggaatcg tcaaactcga accggctctg actgttggca
     2881 aacaactcga cgacgttgcg atgcgagacc gcaacaccct tcggtcggcc agtcgatccc
     2941 gaggtgtaga tcacatacgc actattcgaa tcacgtagcg gaacaacgcg atcatgatca
     3001 gtgattgaag atccgtcgta cttcgacaca tccatggacg cgacgtcgaa cacaggaatg
     3061 tcgagatcga gttcggcccc acccaaaaca cacaccggcc gcgccgcgtc gagcgtgaac
     3121 gcaatccgct cagcgggata gctcggatcg atcggcagat aggcaccacc tgccttcagc
     3181 accgccaaca acgacacgac caagtcaacc gatcgaggca gtaggacagc caccaacgac
     3241 tccggcccca cacctgactc aatcagtttg cgagccaaac gattcgctgc agcgtccaag
     3301 tcgacatacg acactctgcg atcttcgaac accaccgcag tcgacatgcc ccgagccagc
     3361 acttgcgcgt cgaacatcga gaccaacgtc gtatccactt cgggcttcac ccgaacctga
     3421 tcgttccagc cgtccagcac cagcgaacgc tcgaccgaat ccaacaactc cacatcaccg
     3481 acacgaacag ccgaatccgc cgctacggca gtgaggatcc gctcgaatcg ttcagcaaat
     3541 gaggcaaccg tcgacgcgtc gaacagatcc
//
