LOCUS       NZ_JAJNDF010000008     10888 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Rhodococcus qingshengii strain IEGM 746
            NODE_8_length_410288_cov_201.825823, whole genome shotgun sequence.
ACCESSION   NZ_JAJNDF010000008
VERSION     NZ_JAJNDF010000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Rhodococcus qingshengii
  ORGANISM  Rhodococcus qingshengii
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Rhodococcus; Rhodococcus erythropolis group.
REFERENCE   1  (bases 1 to 410288)
  AUTHORS   Elkin,A.
  TITLE     Development of a sustainable strategy for remediation of
            hydrocarbon-contaminated territories based on the waste exchange
            concept
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 410288)
  AUTHORS   Elkin,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2021) LAM, Institute of Ecology and Genetics of
            Microorganisms, Ural Branch of the Russian Academy of Sciences,
            Golev Str., 13, Perm, Perm Kray 614081, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-NOV-2021
            Assembly Method                   :: SPAdes v. 3.14.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 275x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021026075.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 04:42:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,393
            CDSs (total)                      :: 6,335
            Genes (coding)                    :: 6,273
            CDSs (with protein)               :: 6,273
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 21 of 62
            Pseudo Genes (incomplete)         :: 52 of 62
            Pseudo Genes (internal stop)      :: 5 of 62
            Pseudo Genes (multiple problems)  :: 14 of 62
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:48:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 40552
            Orig. end                         :: 51440
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAJNDF010000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10888
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10888
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10888
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5888](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5888)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(979..1692)
                     /locus_tag="LQ424_RS20230"
                     /old_locus_tag="LQ424_20225"
     CDS             complement(979..1692)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019349.5"
                     /locus_tag="LQ424_RS20230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_20225"
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="WP_003940486.1"
                     /transl_table=11
                     /translation="MPDSRIAPTARNIPLSVQIAEQLRSQITSGNWELGEKIPGEHQLV
                     EMTGASRNTVREAIRGLVHAGLLEARPGDGTYVCASSELEIALQRRAAAENVLEIFEAR
                     EALELYAARLAAERALPEDIDRMTRALDGRDAEPDVAKSVEYDLDFHLEMFAASGNQLI
                     TDIYRSLDRSDTMSIPQGKSDKETRQLFIGPWTDDDPHRQLLAAIRDRDPDGAAAAVVR
                     LMDHSRSLFLQNMPK"
     misc_feature    complement(1729..1744)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 21.62"
                     /tool="antismash"
     gene            complement(1912..3030)
                     /locus_tag="LQ424_RS20235"
                     /old_locus_tag="LQ424_20230"
     CDS             complement(1912..3030)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019745611.1"
                     /locus_tag="LQ424_RS20235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_20230"
                     /product="hypothetical protein"
                     /protein_id="WP_050656070.1"
                     /transl_table=11
                     /translation="MLTADILLSGPRGRRMLLAYAIDAERALRPEPGDDMFRINAHRAA
                     YHLDIGQGTSQVLFGPGAEEARQTIVTAEGVARSLTEISLPDVTAVDLRSALAEAVDAA
                     RYWQDPDGEDVLVATEVMRDELRRVAEHIAGSECARWWSTSLASSDQWSILSRGEESPD
                     TDEVPAAQQLLSEWRTSTLESEARAERDRPVDPAANWSGYWWSNPPTQGSTRALFDGTP
                     AGLWFVEDSMGWERVVVRALRPPTGARIYEIDSAQAWSELCRRFPLEVTAETRQVWYRT
                     TGRSGRWVIPDWVQVAERFEGVHLTVTGYLAAAGTAIEVDTETASVIAGWAPDETYWFT
                     DAATYISEPCAWVLHDGPEVTDQPAWTLESQQ"
     gene            complement(3389..4231)
                     /locus_tag="LQ424_RS20240"
                     /old_locus_tag="LQ424_20235"
     CDS             complement(3389..4231)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940604.1"
                     /locus_tag="LQ424_RS20240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_20235"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_030536480.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.3e-13, bitscore:
                     44.5, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 4.2e-08,
                     bitscore: 27.3, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLVLGATGFIGSAVMKQLVSRDGNAAIGFVRTATAAKQFGREG
                     IATVIGDINESASLRLAMSQASTVICCVSYVGDDEQQCVHVNQHGVQNVASMAADVGVE
                     RLLYVSTAAVYGPGPFRDLPVNGAPLSPFSPASRTRALAEKFVRDAGGLVVRPHLVYGP
                     GDRWFLPTLTRIVSRLDSIIDGGSAVLSVVDVDLLARSIYELSIEQQFRYGSTLHVNEP
                     VPRTVIDLLEQHARETNWTLPQNSIPRADAVKAAAQLSLDMHQIDMISLDHWFRSRLY"
     gene            4318..4962
                     /locus_tag="LQ424_RS20245"
                     /old_locus_tag="LQ424_20240"
     CDS             4318..4962
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940238.1"
                     /locus_tag="LQ424_RS20245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_20240"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_003940238.1"
                     /transl_table=11
                     /translation="MQERAVLTRQAVILGAAKSFEKFGYSASLGTILQHGGVSKGAMYF
                     HFASKEELAHAVIAAQHGMAMEGTRRVAAHSDIAVETLVLVSQEMARQLVTEPIARGGM
                     RLTMEIGSIQGPIEQPYLDWIEAIKQVATQAAEAGDIVPGTDIDALARFVVGSFTGVQI
                     LSEMLTGRTDLYERLSQMWNLVLPTIVREDKLAHVMQLAALAPREWEAPSA"
     gene            complement(5001..5888)
                     /locus_tag="LQ424_RS20250"
                     /old_locus_tag="LQ424_20245"
     CDS             complement(5001..5888)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729740.1"
                     /locus_tag="LQ424_RS20250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_20245"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_021331802.1"
                     /sec_met_domain="AfsA (E-value: 5.5e-19, bitscore: 62.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEYTRTVPRHLVHRHAVAEVFLTDYRIESDSAYQVAVQLPVSHRI
                     FDVSSEQHDPLGVAEAFRQAVVLLCHTRYEIPLHYKFLMETFSFEMLDALDVQSVAEPL
                     SFSMTVDNLTHRDGAVSGVEVSGILSEGNRHVARCNAVARGVSPEGYRRIRQGREDHRP
                     SRRILPSGAAVVGSERVGRLTDGDVLISTDPARHAMFCAPDPRNHSLFDHPVDHIPGMI
                     LFEAARQALRFHIGNPATKIASLSAVFPRFTEWETPCEVSTIPLDASGHVHRVRFTQEL
                     ETTAELEITVADQS"
     gene            complement(6057..6716)
                     /locus_tag="LQ424_RS20255"
                     /old_locus_tag="LQ424_20250"
     CDS             complement(6057..6716)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729739.1"
                     /locus_tag="LQ424_RS20255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_20250"
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_003939969.1"
                     /transl_table=11
                     /translation="MTLSSTVTLYTTHSDREFANAIAAGISARAYSEPQTADMSLLRHH
                     TSDHGRALVLLPLGGVDWQVKELSALEGSVLVGDLSSGNSMIRGLAAGAHAVLNSQLPF
                     ASLLDELQAAINPNSARSAHDKSSLVQRIRLRTEEFARISSLTDREFEIVGLLYGGSNV
                     DSIARTAHIAPTTVRAHIRSILQKLGVHSQLEACAMLRRSGHDARRHPLELRIGRF"
     gene            7234..8733
                     /locus_tag="LQ424_RS20260"
                     /old_locus_tag="LQ424_20255"
     CDS             7234..8733
                     /EC_number="1.14.13.-"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="other (smcogs) SMCOG1092: hypothetical
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019745606.1"
                     /locus_tag="LQ424_RS20260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_20255"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_003940522.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.8e-09, bitscore:
                     31.9, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQHVDVLIIGAGLSGIGAACHLIREQTGSTYAILERRENIGGTW
                     DLFKYPGIRSDSDMLTFGFGFRPWIGTKVLADGASIRDYVEETAKEYGVTDHINFGRKV
                     VAMDFDRATAQWSVTVLVEATGETETWTANVLVGACGYYNYDKGYRPAFPGEDDFRGQI
                     VHPQHWPEDLDYTGKKVVVIGSGATAITLIPSMAPTAGHVTMLQRSPTWIQALPSEDPV
                     AKGLKLARVPDQIAYKIGRARNIALQRASFQLSRTNPKLAKKLFLTQIRLQLGKNVDLR
                     HFTPNYNPWDQRLCVVPNGDLFKVLKSGKADIVTDRIATFTEKGIVTESGREIEADVIV
                     TATGLNVQILGGATMSIDGEPVKLNETVAYKSVLYSDIPNFLMILGYTNASWTLKADLA
                     ASYLCRVLKIMRKRDYTTFEVHAEPEDFAEESLMGGALTSGYIQRGDGEMPRQGARGAW
                     KVVNNYYRDRKLMHDAEIEDGVLQFSKVDIAVVPDSKVASA"
     gene            complement(8836..9294)
                     /locus_tag="LQ424_RS20265"
                     /old_locus_tag="LQ424_20260"
     CDS             complement(8836..9294)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940318.1"
                     /locus_tag="LQ424_RS20265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_20260"
                     /product="hypothetical protein"
                     /protein_id="WP_042447140.1"
                     /transl_table=11
                     /translation="MEPLEPMRPVSVAVDTRIKTPLWKMVVLYPAVTSVFMFAALTTRT
                     GIGLVVLGLAIFVVGATTYAMSERRMLRENSGIRVPYFAGPPVAPRHVDLLAAAGMPLL
                     TSGAVLTVRASETERPWVFISVLVIAMVLAITVPMVVHNARVKRTESA"
     gene            9372..10061
                     /gene="lexA"
                     /locus_tag="LQ424_RS20270"
                     /old_locus_tag="LQ424_20265"
     CDS             9372..10061
                     /EC_number="3.4.21.88"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009432 - SOS response [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="lexA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940361.1"
                     /locus_tag="LQ424_RS20270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_20265"
                     /product="transcriptional repressor LexA"
                     /protein_id="WP_174519486.1"
                     /transl_table=11
                     /translation="MSGPLTTILIVTDYDDLDMFGGLDASSLPLRQQQILMTIRDLVAA
                     NGCTPSTRQIGDAVGLRSTSTVSKHLKSLEEKGFLRRGAAMARQLDVRPFLVGAKEQSS
                     SNTVTVPVVGDIAAGAPILAEEHADEVLALPRELVGSGTVFGLRVRGESMVDAAICDGD
                     VVVVRRQDEAHSGEIVAAMIDGEATVKVLRRRDGHVYLEPRNPAYSVIDGDRAVILGKV
                     VSVMRRI"
     gene            complement(10075..10488)
                     /locus_tag="LQ424_RS20275"
                     /old_locus_tag="LQ424_20270"
     CDS             complement(10075..10488)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003939933.1"
                     /locus_tag="LQ424_RS20275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_20270"
                     /product="cupin domain-containing protein"
                     /protein_id="WP_042447137.1"
                     /transl_table=11
                     /translation="MEILKPQPTSKAPSDWFTGDVWWDVIYAGQEPSRMRANMVRFAPC
                     ARTDWHSHALGQTLHIVSGTALVQARGGEIVEVHPGETIYTPAGEEHWHGAAPDRFMTH
                     LALWEGPGGGSTETTWGDKVTDEEYGGPRAGRR"
ORIGIN
        1 agaactgcca ctgcggcgac cgacaccagc ccgaggacgg ctcctctctg atccttgggc
       61 agtttgtgca ggagcactgc tgtgaggagg ctgccgacga tcgagaatat ttgcatgatg
      121 ccttgatgca gtccggcggt agccgcggta gtgccgtgtg attcttcaat ggtgggccac
      181 catgtcagaa cggtgtagaa gaagagagat tggacgccca tgaatattgt gatctgccac
      241 ccgagtgctg agcgccaggg ggagcgatgg ccgcctggat gggaatccaa agccgcagca
      301 tgatcgtgtt tcggaagcgt gcgtcggcga agttggggaa ggaacacccc aaatcctatg
      361 agagccatag cagcccagat tccgaatgcg atccgccaac cgtgatcggt gagaccggcg
      421 atcggcacgg caagtccgga tgcggttgcg gcgacggctg cagagaccgc cgagaagatg
      481 ccggtcaaac ggccggcttc ggtcgggaag tctcgtttga ggagcgccgg cagtagaacg
      541 ttcattgttg cgatggacat gccgagcgat acggtaccga tccacagcgc aggcatccac
      601 ggaacagagc ggatcacgat cgctaaggcg agcatgccga gtgctgctgc gagtgtccgt
      661 tccattcccc accgagctgc gatgaccggg gcaacggggg agaacactgc gaagcacagc
      721 agtggaaggc tgatcagcgc tgatgctgtt gtcgacgaga tatcgagatc ggtgcgtacc
      781 tcaccgagta gcgggccgac gactgtgatt gaggctcgaa gattggctgc gatgagaaga
      841 atgccagcga taacgagtcc tcggcgcatt atcggtatcg cgctcggggc agacgccacg
      901 ttttcggtgt cggagatgac gttagccata ggtgtgccct tctgagattg tctgtgcgct
      961 gaacctcttg tgtgtcagct acttcggcat gttttgcaga aagagggatc ggctgtggtc
     1021 catcagtcgg accactgcgg cggctgcgcc gtccggatcg cggtcacgga tggccgcaag
     1081 cagctgccgg tgtgggtcgt cgtcggtcca aggtccgatg aacagctgtc gagtctcctt
     1141 gtcggactta ccttgtggga tgctcatggt gtcagagcga tcgagactgc ggtagatgtc
     1201 cgtgatcagt tggttcccgc tcgctgcgaa catctccagg tggaagtcga ggtcgtactc
     1261 gacggatttg gcgacgtcgg gctcagcgtc tcggccatcc aaagcccgag tcattcggtc
     1321 gatgtcttcg ggcagggctc gttcggctgc gagtcgggcg gcgtagagct ccaaagcttc
     1381 acgggcttcg aagatttcga gcacgttctc cgccgctgca cgtctttgaa gtgcaatttc
     1441 gagttctgag ctcgcgcaga cgtaggttcc gtctcccggg cgtgcttcga ggaggccggc
     1501 atggacgagg ccgcgaatgg cctcccggac cgtattgcgg ctcgcgccgg tcatttcgac
     1561 aagttgatgc tcgccgggga tcttctcccc taattcccag ttgccagaag tgatctggga
     1621 ccgcagttgt tccgcgatct gcactgaaag tgggatgttg cgtgcggtgg gtgcgatacg
     1681 ggaatccggc atgagtcaaa tgtaggacat ttggcgattt gtggataggt ttacctttgt
     1741 gatctagggc actcgggttc ggatcggcct gggcggggta ccgcccttcc gcatgtctgc
     1801 gtgcacgtga gtgctcctgc cgaccgcgca cggggcagtt gactcgactc gaagggcaac
     1861 tgcgatcgat tcctcacgga acctcatgcc tagcagaccc tcacgtgcgg gtcattgctg
     1921 cgattccagc gtccatgcgg gctggtcggt cacttccggg ccgtcgtgca gcacccaagc
     1981 acacggctcg gaaatgtagg tggcagcatc ggtgaaccag tatgtttcgt cgggtgccca
     2041 gcccgcgatg acgctcgccg tctcggtatc gacctcgatg gcggttcctg ctgccgcgag
     2101 gtatcccgtc acggtaaggt gcactccctc aaatctttcc gcgacctgca cccagtcggg
     2161 gatgacccac ctgcctgatc ggccggtagt ccgataccaa acctgccgcg tttctgcggt
     2221 gacctcgagc gggaaccgtc tgcacagctc cgaccaggct tgcgcgctgt cgatctcgta
     2281 gatgcgtgcg ccagtcggtg gccgcaatgc tcggacgacg acccgttccc atcccatgct
     2341 gtcttcgacg aaccacaagc ccgccggagt cccgtcgaac agcgccctcg tcgaaccctg
     2401 agtgggtgga ttcgaccacc aataacctga ccagttcgcc gcggggtcca ccgggcggtc
     2461 acgttcagct ctcgcttccg attccagggt gctcgtgcgc cactcgctga ggagttgttg
     2521 cgcagcaggc acttcgtctg tgtccggaga ttcctcacct ctgctcagaa tcgaccattg
     2581 gtcggatgag gcgagcgagg ttgaccacca ccgcgcgcat tcggagccgg cgatgtgctc
     2641 ggcgacgcgt cgaagctcat ctcgcataac ttcggtggcg acgaggacgt cttctccgtc
     2701 gggatcctgc cagtaacgcg ccgcatcgac cgcttctgcg agggcggagc gtagatcgac
     2761 ggcggtgacg tcggggagcg atatttccgt gaggctgcgc gccacgccct ccgcggtgac
     2821 tatggtctgc cgggcctctt cggcgcccgg gccgaacaac acttgggagg ttccttgtcc
     2881 gatgtcgagg tgatacgccg cccggtgagc gttgatgcgg aacatgtcgt cgcctggctc
     2941 gggacgcagg gcgcgttcgg cgtcgatcgc atacgcgagc agcatccggc gtccgcgcgg
     3001 gcccgacagc aaaatatccg cagtgagcat ctgttctccc gttttctggt tcagatgacg
     3061 gtagccgagg tcgttgagac ggagaacttt ctcctttgat gtcggaaatc ccgacggtcc
     3121 cgtcgcgcac gacatgatcg ccacacactg cattcggcgg cgaaggcgga cggaagggag
     3181 ccccaggctg ctgccagtcg cgtgaacgcc gcttctttgg tgtgcgcgat cactgccgtc
     3241 tttgtgtagc cgtggttttc gtcgatcaga gctctctgtt tgcctgtagt gactgcgaaa
     3301 taggtcgtat tgattgctga ggctgaacaa tggagagggc tcaaaaggtt gggagacagg
     3361 tgttttggcg agagtgatcc gtgtgccatc agtacagtct cgaccggaac cagtgatcga
     3421 ggctgatcat gtcgatctgg tgcatgtcga gactcagctg ggcagcagct ttcaccgcgt
     3481 cggcgcgggg gatagagttc tggggcagag tccagttggt ctcccgtgca tgttgctcga
     3541 gaaggtcgat gactgtccgc gggacaggct cgttgacgtg aagtgttgag ccgtagcgaa
     3601 attgctgttc tatcgacaac tcgtagatgg atcgggcgag taggtctacg tcgactaccg
     3661 acaggacagc gctaccgccg tcgatgatcg agtcgaggcg agagacaatt cgcgtcagtg
     3721 tcggaagaaa ccatctgtcg ccgggtccgt acacgagatg cggccggacg accaggcctc
     3781 cggcgtcgcg cacgaacttc tcggcgagag cgcgcgtgcg actggcaggt gagaaggggc
     3841 tgagaggtgc gccgttgacc ggcaagtctc gaaacggtcc cggcccgtac acggccgcgg
     3901 tactgacgta gagcaagcgt tcgacgccga cgtcggcggc catcgacgca acgttctgta
     3961 cgccgtgttg gttgacgtga acgcactgtt gttcgtcgtc gcccacgtac gacacgcaac
     4021 agatgacggt cgacgcctga ctcattgcaa gtcgtagaga agcactttcg ttgatgtcgc
     4081 cgatcacggt ggcgattcct tcacggccga actgtttggc tgcggtggcc gtgcgaacga
     4141 aaccgattgc agcgtttccg tcgcgggaaa ccagctgttt catcactgcg gagccgatga
     4201 aaccagtggc cccgagtacc agagtcgtca cggtgtgtcc gtgtcgagag cgttgctgcg
     4261 ggctctttcg acgtatcgtt gagattcatt ccgtcggcaa cgagagctgg gtagatcatg
     4321 caggagcgtg ctgtcctgac gcgccaggcc gtcattttgg gtgctgcgaa gagcttcgag
     4381 aagttcgggt actccgccag tttgggcacg atcttgcagc acgggggtgt gtcgaagggg
     4441 gcgatgtatt ttcatttcgc gtccaaggag gagcttgcgc acgcggtcat cgcagcgcag
     4501 cacggtatgg cgatggaggg tacccggcgg gttgctgcgc actccgacat cgcggtcgaa
     4561 accctggtgc tcgtttcgca ggagatggct cggcagcttg tgaccgagcc catcgcgcga
     4621 ggtggcatgc ggttgacgat ggagatcggc agtattcaag gccccatcga acagccgtac
     4681 ctcgattgga tcgaggctat caagcaggtg gcaacccaag cggccgaagc cggcgatatc
     4741 gtcccgggca ccgatataga cgcgttggct cgatttgtgg tggggtcgtt caccggagtg
     4801 cagattctct cggaaatgct gacgggcaga accgatctgt acgagcggtt gagtcagatg
     4861 tggaacctgg tgctgccaac gatcgtccgc gaggacaagt tggcgcacgt catgcaactg
     4921 gcggcgcttg caccgagaga gtgggaagca ccgagcgctt gacgtggtag acgcgacgag
     4981 aagaacgtgt tcgttccctc tcatgattgg tccgcaactg taatttcgag ttccgcggtg
     5041 gtttccagtt cttgagtgaa ccgaactcgg tgcacatggc ccgacgcgtc aagggggatc
     5101 gttgacacct cgcacggcgt ttcccattca gtgaacctcg gaaagaccgc cgagagtgag
     5161 gcgatctttg ttgctgggtt gccgatgtga aatcggagcg cttgtcgggc ggcctcgaaa
     5221 agtatcattc ctgggatgtg gtcgacgggg tggtcgaaaa gggagtggtt gcgcggatcg
     5281 ggagcgcaga acatcgcgtg ccgagcagga tcagtgctga tgagcacatc tccatcggtt
     5341 aggcgtccga ctcgctccga tccgaccacc gcggctccgg aaggaaggat gcgtcggctc
     5401 gggcggtggt cttctcgtcc ttgccgaatt cgacggtatc cctcggggct gactcctcgc
     5461 gcaacagcgt tgcatctggc gacgtgccgg ttgccctcgc tgaggatccc cgatacttcg
     5521 acccccgaaa ccgctccgtc gcgatgagtg aggttgtcca ctgtcatcga gaaactcaac
     5581 ggctccgcga ctgattgcac gtcgagcgcg tccagcatct cgaagctgaa cgtttccatg
     5641 aggaacttgt agtgcagtgg aatctcgtat cgggtgtggc acagaagcac cactgcctgt
     5701 cgaaatgctt ctgccacccc gagcggatcg tgttgctcgc tcgatacgtc gaatatccgg
     5761 tgcgagaccg ggagttgcac agcaacctgg tacgccgagt ccgattcgat ccggtagtcc
     5821 gtcaagaaaa cctcggcaac agcatgtctg tggacgagat gccgcggcac tgtgcgtgtg
     5881 tattccattg attgaatgca cctttcgatg ttcactcgtg tcttcggtgt cgtgtaggac
     5941 ctaatattcg acgtccgggc gcgtcatcga atcgtcaaaa ttgacgatct tttcgaaggt
     6001 cgagttcgat caggggcggc accccatctc ggcgagtgtg tctggggcac ttcctgctag
     6061 aagcgtccga tccgaagttc cagtgggtgt ctgcgtgcgt cgtgaccaga gcggcggagc
     6121 atcgcgcatg cctccagctg ggaatgcacc cccagttttt gcaggatgct tcggatgtgg
     6181 gcgcgaaccg tggtcggtgc gatgtgggcg gttcgagcaa tcgagtcgac attcgagcct
     6241 ccgtagagaa ggcccacgat ctcgaactcg cgatcggtga gactgctgat tcgtgcgaat
     6301 tcttcggtgc gcaaccggat gcgttgtacg agcgaggact tgtcgtgtgc agaccgagcg
     6361 gagttggggt tgatcgcggc ctgcagctca tcgagcaggg atgcgaatgg cagttgagag
     6421 ttgagcacgg cgtgggcgcc cgctgcgagc ccccgaatca tggagttgcc gctactgaga
     6481 tcgccgacca ggaccgatcc ctcgagcgcg gagagctcct tcacctgcca atcgacgccc
     6541 ccgagtggta ggagaacgag cgctctaccg tgatcgctgg tgtgatgcct taacaggctc
     6601 atgtctgcgg tttgtggctc ggaataagcc ctggcgctga ttccggcagc gattgcattg
     6661 gcgaactctc gatcgctatg agtggtgtac agggtgactg tgctggagag tgtcatagcg
     6721 actacatctc cggtggaggt ccactccgcc cgctctcttc gctgcggaac caaaaccgtt
     6781 gctttcagcg tggtttcgtc agcaggttag ctatcgcagt gacctttccg ctgatgcgca
     6841 cgaaggttac ggcgatatcg cagttgcccg tcctcccatg gaaactcgcg cgttcgacac
     6901 ctgatcgtgg accttcgaat tatgctaggg cgctgaatat ttgggcgatg agtatcccgc
     6961 gtagacgagc cattaggcgt tccgtcgacg acgtgaacga ctccccaatg gctcgaggca
     7021 ttcaggcgtc tcgatcgagt ttccgcgagg ccggtgcgtc gcgcctcaat gttccgtacg
     7081 cggccgggtc gagatattga ctgttaccgt cagttgcaca taccctgttc attggcgctg
     7141 accttaggga acccggcctg aaccggccga tcgttctcgg tcactgccga cgccagaagt
     7201 agcttcgcga cgaccgacga aacgagtttc ctcatgacac agcatgtcga cgtactgatc
     7261 atcggcgctg gcttgtccgg aatcggcgcg gcttgccacc tcattcgtga gcagaccgga
     7321 agcacttacg cgatcctcga acgccgcgag aacatcggtg gcacctggga cctgttcaag
     7381 tatcccggca tccgatcgga ttccgacatg ctcaccttcg gattcggttt ccgtccgtgg
     7441 atcggaacca aagtgctcgc agacggcgcc agtatccgtg actacgtcga ggaaaccgcc
     7501 aaggaatacg gcgtcaccga ccacatcaac ttcggccgca aggtcgtggc aatggacttc
     7561 gatcgcgcga ctgcacagtg gtccgtgacc gtcctggtcg aggcgacggg ggagaccgag
     7621 acgtggaccg cgaacgtcct cgtcggcgcc tgtggttact acaactacga caagggatac
     7681 cgccccgcct tccccggtga ggacgacttc cgcggtcaga tcgtgcaccc gcagcactgg
     7741 ccggaggatc tcgattacac cggaaagaag gtagtggtca tcggttccgg tgccaccgcg
     7801 atcaccctga tcccgtcgat ggcccccacc gccggtcacg tcaccatgct gcagcgctcg
     7861 cccacgtgga tccaggcgct gccgtccgag gaccctgttg ccaagggtct caagcttgcg
     7921 cgcgttcccg accagatcgc ttacaagatc ggtcgagccc gcaatatcgc actgcaacgc
     7981 gccagctttc agctctcacg caccaatccg aagctggcga agaagctgtt cctcacccag
     8041 atccgcttgc aactcggcaa gaacgtggac ctgcgtcact tcacccccaa ctacaacccg
     8101 tgggatcagc gcctgtgcgt ggttcccaac ggggacctgt tcaaggtgct caagagcggc
     8161 aaggccgaca tcgtcaccga ccgtatcgcc acgttcaccg agaagggcat cgtgaccgag
     8221 tcgggccgcg aaatcgaggc cgacgtcatc gtcacggcga ccggcttgaa cgtacagatc
     8281 ctgggcgggg cgaccatgag catcgacggc gagcccgtca agctcaacga gacggtcgcc
     8341 tacaagagcg tgctctactc cgacatcccg aacttcctga tgatcctcgg ctacaccaac
     8401 gcatcctgga cgctcaaggc cgacctggcc gcgtcctatc tgtgtcgcgt actcaagatc
     8461 atgcgtaagc gcgactacac gactttcgag gttcacgccg aacctgagga cttcgcggaa
     8521 gaatctctca tgggcggagc cctgacctcg ggctacatcc agcgcggcga cggagaaatg
     8581 ccgcgtcagg gtgcccgcgg cgcgtggaaa gtggtcaaca attactaccg cgaccgcaag
     8641 ctgatgcacg acgccgagat cgaagacggt gtgctgcagt tcagcaaggt cgatattgct
     8701 gtcgtgcctg atagcaaggt cgccagcgca tagtcggctc caccggggga tggaccggtc
     8761 gcaccaccca gacgtcggtc cgacgtctgg gaaggctgaa cgtgacgctg caagatgtcg
     8821 gtatggcgtg actgctcagg ctgattctgt ccgcttgacc cgcgcgttat gcacaaccat
     8881 cgggacagtt attgccagga ccatcgctat cacgagcacc gatatgaaaa cccagggacg
     8941 ttctgtctcg gatgctcgga cggtcaacac agctccactc gtaagcaggg gcatccctgc
     9001 tgccgcgagt agatcgacgt gccgcggggc gacgggaggt ccggcgaagt atggaactcg
     9061 aatccctgag ttctcccgaa gcatccgtcg ctccgacatc gcgtaagtgg tcgcgccgac
     9121 tacgaaaatg gccagtccga gtacgacaag accaattccc gttcgggtgg tgagcgcggc
     9181 aaacatgaac accgatgtca ctgccggata gagcaccacc atcttccaga gtggagtctt
     9241 gatccgcgtg tcgactgcaa cgctgaccgg gcgcatcggt tcgagaggct ccatcagctc
     9301 agcgtgccat gcgccgctcg ggaaacaaac tgcctgtgcc gtttctggca gactccggct
     9361 gcctggcctg gatgtcggga cccctcacta cgattctcat cgtgaccgat tacgacgatc
     9421 tcgacatgtt cggtggcctc gacgcctcct ccctgccgct ccgccagcag cagattctga
     9481 tgaccatccg ggatctcgtg gctgccaacg ggtgcacgcc gagcacacgg cagatcggag
     9541 acgcggttgg tctgcgctcg acgtcgacgg tgtccaagca cctcaagagt ctcgaagaga
     9601 agggcttcct ccgtagggga gcggccatgg ctcggcagtt ggacgtgcgg ccgttcctcg
     9661 taggcgcgaa ggaacagtcg tcgtcgaata cggtgacggt tccggtcgtc ggtgacatcg
     9721 cggccggcgc gccgatcctc gcggaggagc acgccgacga ggtgcttgcg cttcctcgcg
     9781 agctcgtcgg ctccggcacg gtgttcggac tccgggtgcg tggtgagtcg atggtcgacg
     9841 cagcgatctg tgacggtgac gtcgtcgtgg tgcgccgcca ggacgaagct cattccggtg
     9901 agatcgtcgc cgcgatgatc gacggcgagg ccacggtgaa ggtgctccgg cgtcgcgacg
     9961 gtcacgtgta cctcgagcct cgcaatcccg cgtacagcgt catcgacggc gatcgcgcgg
    10021 tcatcctggg caaagttgtt tccgtgatgc gccgtatctg agaggtgtcc ggtctcaacg
    10081 acgtccggcg cggggaccgc cgtattcctc atcggtgact ttgtcgcccc atgtagtttc
    10141 ggtgcttccg ccgccgggtc cttcccacag cgcaaggtgg gtcatgaaac ggtcgggtgc
    10201 tgcgccgtgc cagtgttcct ctcctgccgg ggtatagatg gtctcgcccg gatgaacttc
    10261 gacgatctcg ccgccgcgag cttgaaccag cgcggtcccg gagacgatgt ggagggtctg
    10321 tccgagggcg tgggaatgcc agtcggttcg cgcgcacggt gcaaagcgaa ccatgttggc
    10381 gcgcatcctc gacggctcct ggccggcgta gatgacgtcc caccagacgt cgccggtgaa
    10441 ccagtcgctg ggtgctttgc tggtgggctg gggcttcaag atttccatga ggtctcctcg
    10501 gaatcgaaag gggcggttca gttctgcgtc agcggaacaa gtcgaatggt gttgggtgcg
    10561 gcggcgcggc cggtgacgga gccgacgatc tgcgggccgt agcggtcgta cttggtgtga
    10621 taggcggagt cgatcgacga gtgggcatcc ggtgcggcgg actcgaacgc cacgtcccgc
    10681 tcgattcctc ctgctcggat tcggcccgtt ccacttgccg ttgctcggac gaaccacggg
    10741 ttgtcggttg cgtaggccga gcgaacgtac agttggttgt cggtacggac aacccaaatc
    10801 gtgacgtacg gccttgcggt tccgtctcgg cggtacgagg agacctgcaa ctcctcggcg
    10861 tcgccgatct tggtcagttc agcctcgg
//
