LOCUS       NZ_JAJNDF010000002     10399 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Rhodococcus qingshengii strain IEGM 746
            NODE_2_length_767368_cov_137.515908, whole genome shotgun sequence.
ACCESSION   NZ_JAJNDF010000002
VERSION     NZ_JAJNDF010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Rhodococcus qingshengii
  ORGANISM  Rhodococcus qingshengii
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Rhodococcus; Rhodococcus erythropolis group.
REFERENCE   1  (bases 1 to 767368)
  AUTHORS   Elkin,A.
  TITLE     Development of a sustainable strategy for remediation of
            hydrocarbon-contaminated territories based on the waste exchange
            concept
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 767368)
  AUTHORS   Elkin,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2021) LAM, Institute of Ecology and Genetics of
            Microorganisms, Ural Branch of the Russian Academy of Sciences,
            Golev Str., 13, Perm, Perm Kray 614081, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-NOV-2021
            Assembly Method                   :: SPAdes v. 3.14.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 275x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021026075.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 04:42:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,393
            CDSs (total)                      :: 6,335
            Genes (coding)                    :: 6,273
            CDSs (with protein)               :: 6,273
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 21 of 62
            Pseudo Genes (incomplete)         :: 52 of 62
            Pseudo Genes (internal stop)      :: 5 of 62
            Pseudo Genes (multiple problems)  :: 14 of 62
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:48:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 609197
            Orig. end                         :: 619596
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAJNDF010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="5"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            38..859
                     /locus_tag="LQ424_RS07375"
                     /old_locus_tag="LQ424_07365"
     CDS             38..859
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019749061.1"
                     /locus_tag="LQ424_RS07375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_07365"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_007731010.1"
                     /transl_table=11
                     /translation="MLSIGDFARAGRVSVRMLRHYDAIGLLRPARVDASTGYRSYTAGQ
                     LALLNRIVALKELGLTLAQVQQVVESAVTVDEIRGMLTLRRAELETRIQEDTARLAHVQ
                     ARLRIIESEGQMSAYEVVIKSVPALRVAELTGTAKTMDPSSIGPVVRGLYERLCTGLDR
                     AGIAAIGPAVAYYEDAGDDENVVVHAALPVDANPDGDYEFAVVDLRAEEHVASLLHKGS
                     MDNCLPAYQALAQWIDAAGYRNAGPSREVTLACPEDVDGWVTELQEPVAKA"
     gene            899..2998
                     /locus_tag="LQ424_RS07380"
                     /old_locus_tag="LQ424_07370"
     CDS             899..2998
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248057.1"
                     /locus_tag="LQ424_RS07380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_07370"
                     /product="DEAD/DEAH box helicase"
                     /protein_id="WP_030536630.1"
                     /transl_table=11
                     /translation="MTTATAPAMRDEAERLLRELAGPDARLREDQWTAIEALVVQRKRA
                     LVVQRTGWGKSAVYFISAKLLRATGHGPTVIVSPLLALMRNQVSSAERAGVRAATINSG
                     NVTEWDDIHARVAANDLDVLLVSPERLNNPDFRDQVLPALAADAGLVVVDEAHCVSDWG
                     HDFRPDYRRIRTLIAELGEGIPVLATTATANNRVVEDVSAQLGVGGEETLVLRGGLERE
                     SLHLSVVHIPEATARAAWLADMLETLPGSGIIYTLTVSAARDLASLLSERGHTVAAYTG
                     QTDPTERESLEQDLLGNRVKALVATSALGMGFDKPDLGFVVHLGAPSSPISYYQQVGRA
                     GRSTDRADVILLPGAEDKQIWSYFASVAFPREHLVRSVIEHLDTEKATSTPALEPLVEL
                     NRTRLEMVLKVLDVDGAVKRVKGGWISTGEPWVYDAERYGRLDDARQAEQAAMVEYQSI
                     TTCRMTFLREQLDDPGLTSENSQCGRCDNCTGNRYDTTVDTAALDETRARLQRPGFDLE
                     PRKMWPTGLSKLGVKLSGKITEGPETGRILGRMSDLGWGQRLRALFDGPDAEVPDAVVS
                     ACISVLAAWNWAERPTGVMALESTTHPLLVSSLAAKLAEVGRLTDLGTLRLRPDHPPVS
                     AANSAYRVSGLVDVWEVPDMSIHQGPILLVDAIADTGWTFTMAARALRQAGAHSVLPLA
                     LASPK"
     misc_feature    complement(3048..3063)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     misc_feature    complement(3125..3139)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     gene            3159..3674
                     /gene="ectA"
                     /locus_tag="LQ424_RS07385"
                     /old_locus_tag="LQ424_07375"
     CDS             3159..3674
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003944572.1"
                     /locus_tag="LQ424_RS07385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_07375"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_042450217.1"
                     /transl_table=11
                     /translation="MKPVDTRAIPTTAPDAVLFRSPQIDDGVRLWEIARDSKVLDLNSS
                     YAYLLWCRDFSATSIVAEVDGRVVGFVSGYTRPSAQETLFVWQVAVDEDQRGKAIAARM
                     LSDLMDRTAPLGVTHLETTISPDNEVSIALFTALARRREVPITRQTLFSPNDFPDGHEA
                     EDLYTIGA"
     gene            3723..4985
                     /gene="ectB"
                     /locus_tag="LQ424_RS07390"
                     /old_locus_tag="LQ424_07380"
     CDS             3723..4985
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (27-361). E-value: 6.3e-72.
                     Score: 234.3. Matches aSDomain:
                     nrpspksdomains_LQ424_RS07390_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003944528.1"
                     /locus_tag="LQ424_RS07390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_07380"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_007731008.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.5e-74, bitscore:
                     245.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNINEASIFETLESEVRSYCRDWPAVFTSASGSWIRDENGRDYLD
                     FFAGAGALNYGHNNPVLKSALVDYIMSDGITHGLDMSTVAKREFLETFQRNILEPRGLD
                     YKVQFPGPTGSNTVEAALKLARKVTGRSAVINFTNAFHGMTLGALSVTGNSMKRAGAGV
                     PLVHTTPMPFDNYFDGVTEDFQWFSRVLDDSGSGINRPAAVIVETVQGEGGVNVARPEW
                     LRALATLCSDRGILLIVDDVQMGCGRTGPFFSFEEAGIVPDIVTLSKSISGYGMPMALT
                     LFKRELDVWGPGEHNGTFRGNNPAFVTSKVALDHYWSDDALTKTTLKKGARISECFANL
                     SDQFPGEVSHRGRGLVQGLVFEQPERAGKVCQLAFDEGLLAETSGPSDQVVKLLPALTI
                     TDDELEHGLSILAEATLKVCS"
     aSDomain        3804..4805
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ424_RS07390_Aminotran_3.1"
                     /evalue="6.30E-72"
                     /label="LQ424_RS07390_Aminotran_3.1"
                     /locus_tag="LQ424_RS07390"
                     /protein_end="361"
                     /protein_start="27"
                     /score="234.3"
                     /tool="antismash"
                     /translation="TSASGSWIRDENGRDYLDFFAGAGALNYGHNNPVLKSALVDYIMS
                     DGITHGLDMSTVAKREFLETFQRNILEPRGLDYKVQFPGPTGSNTVEAALKLARKVTGR
                     SAVINFTNAFHGMTLGALSVTGNSMKRAGAGVPLVHTTPMPFDNYFDGVTEDFQWFSRV
                     LDDSGSGINRPAAVIVETVQGEGGVNVARPEWLRALATLCSDRGILLIVDDVQMGCGRT
                     GPFFSFEEAGIVPDIVTLSKSISGYGMPMALTLFKRELDVWGPGEHNGTFRGNNPAFVT
                     SKVALDHYWSDDALTKTTLKKGARISECFANLSDQFPGEVSHRGRGLVQGLVF"
     gene            5001..5399
                     /locus_tag="LQ424_RS07395"
                     /old_locus_tag="LQ424_07385"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009474015.1"
                     /locus_tag="LQ424_RS07395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_07385"
                     /product="ectoine synthase"
                     /protein_id="WP_003944463.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.5e-53, bitscore:
                     175.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTTTEITDTDRDITSEDGNWRSKRIVLGGDRVGFSFHETTIK
                     AGSVNEFHYANHVEAVWLVEGTGKLIDLDNNKEYDLAPGSMYLLNGHERHRVEPDTQMR
                     MLCVFNPPVTGREVHDENGVYPLVEVPA"
     gene            5409..6341
                     /locus_tag="LQ424_RS07400"
                     /old_locus_tag="LQ424_07390"
     CDS             5409..6341
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575166.1"
                     /locus_tag="LQ424_RS07400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_07390"
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="WP_007731007.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 1.6e-48, bitscore:
                     159.7, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATDTSPRVAIGPVPDALLDSAVLRGGGLISPIDTADALIWAGSP
                     ETFPSELPASVRWVQLPSAGIEDWFDSGVIPKDTEITWTSAAGAFSASVAEHALMLLLA
                     GVRHLPAHIEARTWRQFEFFPKIGTLRGSTVAIIGAGGIGRALIPMLAAQGADSIAVNR
                     SGSPVPGAISTYPASRMDEIYSRADHFVIAAPATPATRHLVGRAQLAAMQPHSWVINVA
                     RGSLVDTDALVEAIESEQIGGAGLDVTDPEPLPDGHPLWDLTNAIITPHDSNPPGVRPA
                     AFADHVSKNVTRFAKGEALAALVDPVAGY"
     gene            complement(6386..6907)
                     /locus_tag="LQ424_RS07405"
                     /old_locus_tag="LQ424_07395"
     CDS             complement(6386..6907)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019749065.1"
                     /locus_tag="LQ424_RS07405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_07395"
                     /product="VOC family protein"
                     /protein_id="WP_080726651.1"
                     /transl_table=11
                     /translation="MFNRTRVRSPILVHMNTVNPIPAGYRTITPYLAVPDGHAALDYYK
                     RALGAEIVDAMNAPDGSLMHAEIRIGDSMLQLSQEMPDFGLKSPQDGWVHSSIVVYTED
                     TDEFVARAVTEGGTLVTAVADTFSGDRHGVFLDPFGHRWAVCTKVEDVPAEEVARRARA
                     LFFPETQSPQ"
     misc_feature    6884..6899
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.76"
                     /tool="antismash"
     gene            6958..7815
                     /locus_tag="LQ424_RS07410"
                     /old_locus_tag="LQ424_07400"
     CDS             6958..7815
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730998.1"
                     /locus_tag="LQ424_RS07410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_07400"
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="WP_042450219.1"
                     /transl_table=11
                     /translation="MPTDNSRLDALPELLAGRRLAVLTGAGISTDSGIPDYRGPDSPPR
                     NPMTFQQFIGDPAFRRHYWARNHIGWRYMDSSRPNQGHRAVAALERTGRAVGVITQNVD
                     LLHTKAGARNVIDLHGTYAQVRCLSCQRLISRTTLAERLEEANPGFAETVAAATGVEIA
                     PDADAVIESTAHFRVVDCAVCGGLLKPDIVYFGESVPKERVARAYSLVDQSDALLVAGS
                     SLTVMSGLRFVRHAAKTGKPIAIVNRGTTRGDEFADLKLEVGCAEALSALVEHESYSAT
                     KSSA"
     gene            complement(7788..8891)
                     /gene="proB"
                     /locus_tag="LQ424_RS07415"
                     /old_locus_tag="LQ424_07405"
     CDS             complement(7788..8891)
                     /EC_number="2.7.2.11"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="proB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003944552.1"
                     /locus_tag="LQ424_RS07415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_07405"
                     /product="glutamate 5-kinase"
                     /protein_id="WP_003944552.1"
                     /transl_table=11
                     /translation="MLGSTRESIASAHSIVVKIGSSALTSLVAGLDTHRLDLLADAIEA
                     RMRAGSDVVVVSSGAVGAGLAPLGLTKRPRDLATKQAAASVGQLALAHAWGTSFARYGR
                     TVGQVLLTADDIARRTQHRNAQRTLDRLRALHAVAIVNENDTVATTELRFGDNDRLAAL
                     VAHLVGADALILLSDVDGLYDGDPRKGDATLIPEVNSPEDLDGVVAGSGGALGTGGMAS
                     KLSAARLAADSGVPVLLAAASDAAQALSGADVGTAFAPRPTRLSARRFWVRHAADTSGD
                     LHLDEGAVRAVVSKRRSLLSAGIVGVSGDFHAGDVVSLMGPGEKPVARGVVAFDAAEIE
                     EILGRSTTDLPADMQRPVVHADDLVAL"
     gene            complement(8936..10387)
                     /gene="obgE"
                     /locus_tag="LQ424_RS07420"
                     /old_locus_tag="LQ424_07410"
     CDS             complement(8936..10387)
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="obgE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019749068.1"
                     /locus_tag="LQ424_RS07420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ424_07410"
                     /product="GTPase ObgE"
                     /protein_id="WP_003944579.1"
                     /transl_table=11
                     /translation="MSRFIDRVVLHVSAGKGGNGCASVHREKFKPLGGPDGANGGRGGD
                     VILVVDENIHTLLDFHFHPNAKATNGKQGAGSNREGANGEDLILKVPDGTVVLDTDGNV
                     LADLVGVGSRFDAAQGGRGGLGNAALASKARKAPGFALLGEDGVERDLVLELKSVADVG
                     LLGFPSAGKSSLVSVLSAAKPKIADYPFTTLVPNLGVVSSGDTTFTVADVPGLIPGASE
                     GRGLGLDFLRHIERCAVLAHVIDCATLDPGRDPISDIDALEAELAAYTPALSGDSGLGD
                     LDKRPRVVILNKTDVPEAAELAEMVTPEIEARGWPVFTISAVSREGLRPLTFALAKMVR
                     DYREAHPKPEPKRQVIRPVKVKDSSFTIEKDPDIPGGFVVRGTRPERWIRQTAFDNDEA
                     VGYLADRLARLGVEDALVKKGAQPGASVTIGDVSFEWEPMTPAGIDLTRTGRGTDPRLD
                     QVERIGADERKHARRVRRGLLDEDA"
ORIGIN
        1 gtatcgggtt tgtgagagag aaacggggcg agcaaggatg ttgagcatcg gagacttcgc
       61 gagagcaggt cgtgtgtcgg tgcgcatgct ccgccactac gacgccatcg gtctgctcag
      121 acccgcgagg gtcgacgcat ccaccgggta tcgctcgtac accgcgggcc agttggcgct
      181 gctcaaccgc atcgtcgctc tcaaggagtt gggcctgact ctggcccagg tccagcaagt
      241 ggtggagtcg gcggtcaccg tcgacgagat ccgcgggatg ctcacgcttc gccgagcaga
      301 actcgaaacc cgaatccagg aagacaccgc gcgactggcg catgtacagg cgcgcctacg
      361 gatcatcgaa agtgagggac agatgtccgc atacgaggtt gtgatcaagt cagttccggc
      421 gctgcgggtc gcggagttga ccggtaccgc caagacgatg gatccgtcgt cgatcggccc
      481 ggtggttcgc gggttgtacg agcgcctttg taccggtttg gatcgtgccg gtatcgccgc
      541 gatcggaccc gctgtcgctt actacgagga tgccggtgac gacgagaacg tcgtcgtcca
      601 tgcggcgcta ccggtagacg cgaatcccga tggcgactac gagttcgctg tggtggacct
      661 gcgggccgaa gagcacgtgg cctcgctgct gcacaagggt tcgatggaca actgcctgcc
      721 cgcttatcag gcgttggccc aatggatcga cgcagcggga taccgcaatg ccggccccag
      781 ccgtgaggtg acgctggcgt gcccggagga tgtcgacggc tgggtcaccg aactgcagga
      841 acctgttgcc aaggcctgaa gcccgcacgt gtcggcaggg tcgtgcacca tggggtccat
      901 gaccaccgcc actgccccag ccatgcgcga cgaagccgag cggctgctcc gcgaactcgc
      961 ggggccggat gcccgcctac gcgaggacca gtggacggcc atcgaggcgc tggtggtgca
     1021 gcgcaaacgc gcgctcgtcg tacagcggac cggctggggc aaatctgcgg tctacttcat
     1081 ctcggcgaag ctgctccggg caacgggcca cggccccacc gtcatcgtct ctccgttgtt
     1141 ggccctgatg cgaaaccagg tcagttctgc cgaacgtgcc ggagtgcgcg ccgcaaccat
     1201 caattcgggc aacgtcaccg aatgggacga catccatgcc cgcgtcgcag ccaatgatct
     1261 cgacgtcctt ctggtcagcc ccgagcgcct gaacaaccct gacttccgcg atcaggtgct
     1321 cccggccctg gcagctgatg ccggcctggt cgtagtcgac gaggctcact gcgtttccga
     1381 ctggggccac gacttccgtc ccgactaccg ccgcatcagg accttgatcg ccgaactcgg
     1441 cgagggcatt cccgtcctcg caaccacggc gacggccaac aaccgcgtcg tcgaggacgt
     1501 gtccgcccaa ctcggcgtcg gcggtgagga aaccctcgtc cttcgcggcg ggctcgaacg
     1561 cgaatcgctt cacctctctg tagtccacat tccggaggcg acggcgcgcg ctgcctggct
     1621 tgccgacatg ctcgagacgc tgccgggttc gggcatcatc tacacgctga cggtttcggc
     1681 cgcgcgcgac ctcgcaagcc tgctctccga gcgcggccat accgtcgccg cctacacggg
     1741 ccagaccgat cccactgaac gtgaatctct cgagcaggac ttgttgggta atcgcgtcaa
     1801 ggctctggtg gccacctcgg cactcgggat gggcttcgac aagcctgatc tcgggttcgt
     1861 cgtccacctc ggcgcaccgt cgtcgccgat ctcgtactac cagcaggtgg gccgcgccgg
     1921 tcgttccacc gacagggcag acgtgatcct cctgcctggc gccgaggaca agcagatctg
     1981 gagttacttc gcctcggtcg ccttcccccg cgagcacctg gtccgcagcg tcatcgaaca
     2041 cctcgacacc gaaaaggcca cgtcgacacc ggcactcgaa cctctggtgg agctcaatcg
     2101 cacgcgtctc gagatggtcc tcaaagtcct cgacgtcgac ggcgcggtca aacgcgtcaa
     2161 gggcgggtgg atcagcaccg gcgaaccctg ggtgtacgac gccgagcgct acggccgact
     2221 cgacgacgcc cgccaggccg agcaggccgc aatggtcgag taccagagca tcacgacgtg
     2281 tcgaatgacc ttcctgcgtg aacaactcga cgatcctggt ctgacctcgg agaactcaca
     2341 gtgcggtcgc tgtgacaact gcacaggcaa tcgctacgac accactgtcg acaccgccgc
     2401 cctcgacgag actcgggcgc gcttgcagcg ccccggattc gacctggagc cgcgcaagat
     2461 gtggccgacc ggcctgagca agctgggcgt caagctgtcc gggaagatca ccgaggggcc
     2521 cgaaaccggg cgcattctcg gacggatgtc ggacctgggc tggggtcaac gtctccgcgc
     2581 gcttttcgac ggtcccgacg ccgaggtacc cgatgcggtg gtcagcgcgt gtatcagcgt
     2641 tctggccgcc tggaattggg ccgagcgacc caccggtgtc atggccctcg aatccacgac
     2701 gcatcctttg ttggtgtcga gcctggctgc caagttggcc gaggtcgggc gattgaccga
     2761 cctgggcacc cttcgcttgc gacccgacca ccctccggtg tcggcagcga actccgccta
     2821 ccgcgtgtcc ggactggtcg atgtttggga agtcccggac atgagcatcc accagggacc
     2881 gatacttctt gtcgacgcaa tcgccgatac cggttggact ttcacgatgg ccgcgcgcgc
     2941 acttcgacag gctggtgctc actctgtact tccgttggcg ctcgcaagcc cgaaatagcg
     3001 agtttttgcc cgaaatagtc gctccgacaa cggcaaaagt tacataatgt gagcaccatc
     3061 acattcatgt cctcacctgg ggcgcaacgg gttccacacg gcccgaaacg gacatctgca
     3121 tgacttcgac agcaatgatt gttacgttca gatgaccgat gaagcctgtt gatacgagag
     3181 ctatcccgac caccgcgccg gacgcggtgt tgttcagaag cccacagatc gatgacggag
     3241 tccggctctg ggagatcgca cgtgattcga aggtgctcga cctcaattcg agttatgcat
     3301 acctgttgtg gtgcagggat ttcagcgcga cgtccatcgt cgcggaggta gacggccgcg
     3361 tagtcggatt cgtttccggt tatacccgtc ccagcgcgca ggaaaccctg ttcgtgtggc
     3421 aagttgcggt cgacgaagac cagcgcggca aggcaattgc tgcccgcatg ctgtccgact
     3481 tgatggatcg cactgcccca ctgggtgtta cacatctgga gaccacgatc agccccgaca
     3541 acgaggtttc gatcgcactc ttcaccgcgc tcgcacgtcg gcgcgaagta ccgataacca
     3601 ggcagacctt gttctcgccc aacgattttc ccgatggaca cgaagccgaa gatctttaca
     3661 cgatcggcgc ctagcgcact tcgcgctcgg catacagcat gccccggagg aacaaagaag
     3721 atatgaatat caacgaagca agcatttttg aaaccctcga gtccgaggtt cgcagctact
     3781 gccgtgactg gcccgcagtg ttcaccagcg catccggatc ctggattcgt gacgagaacg
     3841 ggcgcgacta cctcgatttc ttcgccggtg ccggcgccct caactacggc cacaacaacc
     3901 cggtgctcaa gtccgcactg gtcgactaca tcatgagcga cggcatcacg cacggtctcg
     3961 acatgtcgac agtcgccaag cgtgagttcc tcgagacctt tcagcgcaac atcctcgagc
     4021 cgcgcggcct ggactacaag gtccagttcc ccggcccgac cggatcgaac accgtcgagg
     4081 ctgcactgaa gctggcccgc aaggtcaccg gacgttcggc agtcatcaac ttcaccaacg
     4141 catttcacgg aatgacactt ggtgcactct ccgtgaccgg caactcgatg aagcgcgcgg
     4201 gcgccggtgt ccccctcgtg cacacgacac cgatgccctt cgacaactac ttcgacggcg
     4261 tcaccgagga cttccagtgg ttctcccgcg ttctcgatga ttccggcagt ggaatcaacc
     4321 gccccgcggc agtgatcgtc gaaaccgtgc agggtgaagg tggcgtcaat gtcgctcgcc
     4381 ccgaatggct tcgggcgctg gcaacactgt gctcggatcg cggaatcctg ttgatcgtcg
     4441 acgacgtcca gatgggttgc ggccgtaccg gcccgttctt ctccttcgaa gaggccggaa
     4501 tcgttcccga catcgtcacg ttgtccaagt cgatcagcgg ctacggaatg ccgatggcgc
     4561 tcacgctgtt caagcgtgaa ctcgacgtct ggggaccggg cgagcacaac ggcaccttcc
     4621 gcggcaacaa cccggcgttc gtcacctcca aggtggcact cgaccactac tggtccgacg
     4681 acgcactcac caagacgacg ttgaagaagg gcgcacgcat cagcgaatgc ttcgccaacc
     4741 tctccgatca gttccccggc gaggtctcgc accgcgggcg tggcttggtt cagggcctcg
     4801 tcttcgaaca gcccgagcgc gccggcaagg tctgccagtt ggcattcgac gagggattgc
     4861 tcgccgagac ctccggaccc tccgaccagg ttgtgaaact gcttcccgca ctgacgatta
     4921 ccgacgacga gctcgagcac ggcctctcca tcctggccga ggcgacactc aaggtctgca
     4981 gctagaggaa ggactctccc atgatcgttc gcaccaccac cgagatcacc gacaccgacc
     5041 gcgacatcac gagtgaggac ggaaactggc gcagcaagcg catcgtcctc ggcggcgacc
     5101 gcgtcgggtt ctcgttccac gagaccacca tcaaggcggg ttcggtcaac gagttccatt
     5161 acgccaacca cgtcgaggct gtctggctcg tcgagggcac cggcaagctg atcgacctcg
     5221 acaacaacaa ggaatacgat ctcgcaccgg ggtcgatgta cctgctcaac ggccacgagc
     5281 gtcaccgcgt cgagccggac acgcagatgc gcatgctgtg cgtgttcaat ccgccggtca
     5341 ccgggcgtga ggtgcacgac gagaacggtg tctacccact ggtggaggtc cccgcgtagc
     5401 gtctagtcat ggcgactgac acctctccac gcgtagcgat cggccccgtc cccgatgcac
     5461 tgctcgacag cgcggtgctt cggggcggtg gtctgatcag tccgatcgac actgcggacg
     5521 ccctgatctg ggcgggctca cccgagacct ttccgtcgga gttaccggcg tcggtcaggt
     5581 gggtgcagct tccgtcggcg ggaatcgagg attggttcga ctccggtgtg attccgaagg
     5641 acacagaaat cacgtggacc tcggcggccg gtgcgttttc ggcgtcggtg gccgagcatg
     5701 cactcatgct tctcttggcc ggagttcgtc acttgcctgc acacatcgag gctcgaacgt
     5761 ggcgtcagtt cgagttcttt cccaagatcg gcacccttcg tggctcgacg gttgcgatca
     5821 tcggcgcggg cggtatcggc cgcgcgctga ttccgatgct ggccgcgcag ggggcggaca
     5881 gcatcgcagt caatcgttcc ggtagcccgg tccccggggc gatctccacg taccccgcat
     5941 ccagaatgga tgaaatctat tctcgcgcag atcacttcgt gattgctgcg cccgccaccc
     6001 cggccacccg tcacctcgtg ggccgtgcgc agttggcggc gatgcaaccc cattcctggg
     6061 tgatcaatgt cgcgcgcgga tcactggtcg acaccgacgc actggtcgag gccatcgagt
     6121 cggaacagat cggcggcgcc ggtctcgacg tcaccgatcc cgaacccctc ccggacggtc
     6181 acccactgtg ggatctgacg aatgcgatca tcacacccca cgattccaat ccgccaggcg
     6241 ttcgccccgc cgccttcgcg gatcacgtct ccaagaacgt gacccgcttc gcgaagggtg
     6301 aggcgctggc tgccctcgtg gaccccgtag ccggatactg agcacgccgc cgaagctcga
     6361 atgctcggac cacggttgcc acaaattact gtggcgactg cgtctccgga aagaaaagcg
     6421 cccgcgcccg gcgagcgacc tcttcggcag gtacatcctc gaccttcgtg catactgccc
     6481 acctgtggcc gaacggatcg agaaacactc catgccggtc gcccgaaaat gtatcggcca
     6541 cagccgtgac cagcgtgcct ccctcggtga ctgcccgagc gacaaactcg tccgtgtcct
     6601 cggtgtagac gacgattgac gaatgcaccc agccgtcctg cggtgatttg agaccgaagt
     6661 ccggcatctc ctgcgaaagc tgcagcatcg aatcgccgat ccggatctcc gcatgcatga
     6721 gggagccgtc aggtgcgttc atcgcatcga ctatctcagc tccaagtgct cgtttgtagt
     6781 agtcgagtgc ggcatggccg tccggcaccg caaggtaggg agtgatcgtg cggtaaccag
     6841 caggaatcgg gttcactgtg ttcatgtgta cgagtatcgg cgatcgcaca cgtgttctat
     6901 tgaacatttc ggacttcctc cgcgcctccg tctcgcacac gcgcctatcc tggaacggtg
     6961 cctaccgaca acagccgcct cgacgccctc cccgagctcc tggccggccg tcgcctggca
     7021 gttctgacgg gcgccggcat ctccaccgac tccggaatcc cggactaccg cggacctgat
     7081 tcacccccgc gcaatccgat gacgttccaa cagttcatcg gcgatccggc gtttcggcgg
     7141 cactactggg cgcgcaacca catcggctgg cgatacatgg acagctcccg ccccaaccag
     7201 ggccaccggg ccgtcgcggc actggaacga acagggcgag ccgtcggcgt gatcacccag
     7261 aacgtcgacc tcctgcacac caaggccggc gcgcgaaacg tcatcgacct ccacggcacc
     7321 tacgcgcagg ttcgatgcct gagctgccag cggctgatct cccgcaccac tctcgcggag
     7381 aggctggaag aagccaatcc cggattcgcc gaaacagtcg ctgctgcaac aggagtcgaa
     7441 atcgcacccg acgcagatgc cgtgatcgaa tccaccgcac actttcgcgt cgtggattgc
     7501 gccgtgtgcg gtggcttact caaaccggac atcgtctact tcggcgaatc cgtccccaag
     7561 gaacgcgtcg cccgtgcata cagccttgtc gaccaatccg acgcactgtt ggtagccggg
     7621 tcgtcgttga ccgtgatgtc cggcctacgt ttcgtccggc atgccgccaa gaccgggaag
     7681 ccgatcgcca tcgtcaatcg aggcacgact cgcggcgacg aattcgccga cctcaagctc
     7741 gaagtcggtt gcgctgaagc actttccgcc ctcgtcgagc acgaaagcta cagcgccacc
     7801 aagtcgtccg cgtgaaccac aggacgctgc atgtcggccg gcagatcggt ggtggagcgc
     7861 cccaggatct cctcgatctc cgcagcgtcg aacgcaacga caccccgggc gacgggcttc
     7921 tcccccggcc ccatcaggga cacgacgtcg ccggcgtgga agtcaccgga tacaccgacg
     7981 atgccagcgg acagaaggct tcgacgcttg gacacgaccg cgcggacggc gccttcgtcg
     8041 aggtggaggt cacccgatgt gtcagcggcg tgacgaaccc agaatcggcg tgccgacaat
     8101 cgcgtcggcc gcggcgcaaa tgccgttccg acatcggcac cactgagtgc ctgcgcagcg
     8161 tccgatgctg ccgcgagcag aaccggcacg ccggaatcag cggccaggcg tgctgctgag
     8221 agcttcgacg ccatgcctcc ggtaccgagg gcgccacccg aaccggcaac cacaccgtcg
     8281 agatcctcgg gactgttgac ctcgggaatc agtgtggcat cgccctttcg gggatccccg
     8341 tcgtacagtc cgtccacatc cgacaacagg atcaacgcgt cggcgccgac cagatgagca
     8401 accagagcag ccagccgatc gttgtcaccg aatcgaagtt cggtggtggc aacggtgtcg
     8461 ttctcgttca cgatcgccac cgcgtgcaat gcccgtagac gatcgagtgt ccgttgggcg
     8521 ttgcggtgct gcgtccgtcg agcgatgtcg tcagccgtga gtagaacctg cccgacggta
     8581 cggccgtaac gggcgaacga cgtaccccag gcgtgagcca gagccaactg accgacgctg
     8641 gccgcagcct gcttggtcgc gagatcccgc ggtcgcttgg tcagtcccag cggcgccagt
     8701 ccggcgccga cggcaccgga cgagacgacg acgacatcgg aacccgcacg catgcgagcc
     8761 tcgatggcat cggccaacag atcgagtcgg tgagtatcga gtcccgccac cagactggtg
     8821 agcgcagacg acccgatctt cacgacgata ctgtgagccg acgctatcga ctccctggtg
     8881 gacccgagca cagtcccgtt ccctctcttc ggttttcgtc ctgaaatcgc gtgggtcagg
     8941 cgtcttcgtc gagcaagccg cggcggacac gacgcgcgtg cttgcgctcg tccgcgccga
     9001 tgcgctccac ctgatcgaga cgcgggtcgg ttccacggcc cgtgcgggtc agatcgatac
     9061 cggccggcgt catcggctcc cactcgaagc tgacgtcacc gatggtgacg gaagctcctg
     9121 gctgagcgcc cttcttgacc aatgcgtcct cgacgcccaa tcgagcaaga cggtcggcga
     9181 ggtaaccaac ggcctcgtcg ttgtcgaagg cggtctgacg aatccaccgc tccgggcggg
     9241 tgcctcggac gacgaaccca ccgggaatat cgggatcctt ctcgatggtg aagctggaat
     9301 ccttgacctt gaccgggcga atgacctggc gcttcggctc gggcttcgga tgagcttcgc
     9361 ggtagtcgcg gaccatcttc gccaacgcga atgtcaacgg tcgcaatcct tctcggctca
     9421 ccgccgagat ggtgaagacc ggccatccgc gagcttcgat ctcaggcgtg accatttcgg
     9481 ccaattcggc ggcctcggga acgtcggtct tgttgaggat gacgacgcgc gggcgcttgt
     9541 cgaggtcacc gagtcccgaa tcgccggaga gcgcaggcgt gtacgcagcc agctctgctt
     9601 ccagggcgtc gatgtcggag atcgggtcac gtcccggatc gagggtggcg cagtcgatca
     9661 cgtgcgccaa tacggcgcag cgttcgatgt ggcgcaggaa gtccaggccc aaaccgcggc
     9721 cttcgctggc accggggatc agcccgggca cgtcggcgac ggtgaaggtg gtgtcgccgc
     9781 tcgagacgac gccgaggttg gggaccagag tggtgaaggg gtagtcggcg atcttcggct
     9841 tggccgcaga gagaacggac acaagcgaag acttaccggc ggaggggaat ccgagcagac
     9901 cgacgtcggc taccgacttg agttcgagaa ccaggtcacg ctcgacaccg tcctcaccga
     9961 ggagtgcgaa accgggagct ttgcgggctt tcgaggcgag tgctgcgttg ccgagtccgc
    10021 cgcggccgcc ctgagcggcg tcgaagcgtg aaccgactcc cacgaggtcg gcaagcacgt
    10081 tgccgtcggt atcgagaacc acagttccat ccggaacctt gaggatgagg tcttcaccgt
    10141 ttgcaccctc gcggttactg ccggcgccct gcttgccgtt ggtcgccttc gcgttgggat
    10201 ggaagtgaaa gtcgaggagg gtgtggatgt tctcgtcgac cacgaggatg acgtcgccac
    10261 cgcgacctcc gttggcaccg tccggaccgc cgagcggttt gaacttctcg cggtgtacgg
    10321 aggcgcaacc attgccgcct tttccagcgc tgacgtgcag cacaacacgg tcaatgaatc
    10381 gggacatgac tgccggatc
//
