LOCUS       NZ_JAJFDG010000020      9854 bp    DNA     linear   CON 13-JAN-2024
DEFINITION  Bacillus sp. LBG-1-113 seq20, whole genome shotgun sequence.
ACCESSION   NZ_JAJFDG010000020
VERSION     NZ_JAJFDG010000020.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. LBG-1-113
  ORGANISM  Bacillus sp. LBG-1-113
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Gao,J. and Zhang,X.
  TITLE     genome sequence of strain LBG-1-13
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   Gao,J. and Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2021) Beijing Agro-Biotechnology Research Center,
            Beijing Academy of Agriculture and Forestry Science, No. 9 Ban Jing
            Shu Guang Hua Yuan Middle Road, Beijing 100097, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/13/2024 03:11:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,238
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 3,996
            CDSs (with protein)               :: 3,996
            Genes (RNA)                       :: 99
            rRNAs                             :: 8, 8, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 1 (5S, 23S)
            partial rRNAs                     :: 8, 2 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 79 of 143
            Pseudo Genes (incomplete)         :: 88 of 143
            Pseudo Genes (internal stop)      :: 30 of 143
            Pseudo Genes (multiple problems)  :: 47 of 143
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:33:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9854
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAJFDG010000020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..9854
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:8310](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..8310)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..2082)
                     /locus_tag="LJF29_RS21015"
                     /old_locus_tag="LJF29_21010"
     CDS             complement(<1..2082)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (19-305). E-value:
                     9.4e-106. Score: 345.1. Matches aSDomain:
                     nrpspksdomains_LJF29_RS21015_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (471-690). E-value: 1.6e-60.
                     Score: 196.7. Matches aSDomain:
                     nrpspksdomains_LJF29_RS21015_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="LJF29_RS21015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJF29_21010"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_324255133.1"
                     /sec_met_domain="Condensation (E-value: 3.2e-99, bitscore:
                     320.1, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.1e-55, bitscore:
                     177.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEESAASGGYHMLKPADKQDVYPLSSAQKRMYVLNQLDRQTISYN
                     MPSVLLMEGELHISRLRESLNQLVNRHESLRTSFTEADGEPVQRIVEEASIDLHVFDAE
                     EGEAERKIKEFIRPFDLSNAPLIRAALLRMADQKHLLLLDMHHIIADGVSRGIFVKELA
                     LLYKGEQLPEPKLHYKDFAVWQNEAGQKERMKEHEQYWLSVLSGELPELDLPLDYTRPP
                     VQSFEGDTVRFRAGSGTAKAIEKLLAETGTTLHMVLHTVFHVFLSKISGQRDIVIGSVT
                     AGRTNADVQNMPGMFVNTLALRMEAGEKQTFAELLEQAKETNLSALEHQEYPFESLVNQ
                     LDLPRDMSRNPLFNVMVTTENPDKEQLELQDLTISPYEAHQGTSKFDLTLGGFTDENGI
                     GLQLEYATDLFSRETAEKWSEYVLRLLQAVAENPNQPLSSLSLVNETEKQTLLEAWKGK
                     TLPVPTDKTVHQLFEETVQRHKDRPAVTYNGQSWTYGELNAKANRLARILIDCGISADE
                     RVGILTKPSLEMSAAVLGVLKAGAAFVPIDPDYPDQRIEYILQDSGATLLLKQEGITVP
                     DSYTGEVILLDGNRTILSLPLDENDEEDPQTDTTADHLAYMIYTSGTTGQPKGVMIEHH
                     ALVNLCFWHHDAFGMTAEDRSAKYAGFGFDASIWEMFPTWTIGAELHVIDEAIRLDITR
                     "
     source          1..9854
                     /collection_date="2016-07"
                     /country="China: Gansu"
                     /db_xref="taxon:2886094"
                     /host="lily"
                     /isolation_source="root"
                     /mol_type="genomic DNA"
                     /organism="Bacillus sp. LBG-1-113"
                     /strain="LBG-1-113"
                     /submitter_seqid="seq20"
     region          1..9854
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9854
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     aSDomain        complement(13..669)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJF29_RS21015_AMP-binding.1"
                     /evalue="1.60E-60"
                     /label="LJF29_RS21015_AMP-binding.1"
                     /locus_tag="LJF29_RS21015"
                     /protein_end="690"
                     /protein_start="471"
                     /score="196.7"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FEETVQRHKDRPAVTYNGQSWTYGELNAKANRLARILIDCGISAD
                     ERVGILTKPSLEMSAAVLGVLKAGAAFVPIDPDYPDQRIEYILQDSGATLLLKQEGITV
                     PDSYTGEVILLDGNRTILSLPLDENDEEDPQTDTTADHLAYMIYTSGTTGQPKGVMIEH
                     HALVNLCFWHHDAFGMTAEDRSAKYAGFGFDASIWEMFPTWTIGAELHVIDEAIRL"
     aSModule        complement(13..2025)
                     /domains="nrpspksdomains_LJF29_RS21015_Condensation_LCL.1"
                     /domains="nrpspksdomains_LJF29_RS21015_AMP-binding.1"
                     /incomplete
                     /locus_tags="LJF29_RS21015"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(181..240)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21015_0007"
                     /evalue="5.20E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="LJF29_RS21015"
                     /protein_end="634"
                     /protein_start="614"
                     /score="34.2"
                     /tool="antismash"
                     /translation="AYMIYTSGTTGQPKGVMIEH"
     CDS_motif       complement(442..483)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21015_0006"
                     /evalue="6.20E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="LJF29_RS21015"
                     /protein_end="547"
                     /protein_start="533"
                     /score="19.2"
                     /tool="antismash"
                     /translation="LGVLKAGAAFVPID"
     CDS_motif       complement(1012..1125)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21015_0005"
                     /evalue="1.10E-17"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="LJF29_RS21015"
                     /protein_end="357"
                     /protein_start="319"
                     /score="56.4"
                     /tool="antismash"
                     /translation="KETNLSALEHQEYPFESLVNQLDLPRDMSRNPLFNVMV"
     aSDomain        complement(1168..2025)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJF29_RS21015_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="9.40E-106"
                     /label="LJF29_RS21015_Condensation_LCL.1"
                     /locus_tag="LJF29_RS21015"
                     /protein_end="305"
                     /protein_start="19"
                     /score="345.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="DVYPLSSAQKRMYVLNQLDRQTISYNMPSVLLMEGELHISRLRES
                     LNQLVNRHESLRTSFTEADGEPVQRIVEEASIDLHVFDAEEGEAERKIKEFIRPFDLSN
                     APLIRAALLRMADQKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPKLHYKDFA
                     VWQNEAGQKERMKEHEQYWLSVLSGELPELDLPLDYTRPPVQSFEGDTVRFRAGSGTAK
                     AIEKLLAETGTTLHMVLHTVFHVFLSKISGQRDIVIGSVTAGRTNADVQNMPGMFVNTL
                     ALRME"
     CDS_motif       complement(1174..1263)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21015_0004"
                     /evalue="5.80E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="LJF29_RS21015"
                     /protein_end="303"
                     /protein_start="273"
                     /score="47.6"
                     /tool="antismash"
                     /translation="DIVIGSVTAGRTNADVQNMPGMFVNTLALR"
     CDS_motif       complement(1522..1554)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21015_0003"
                     /evalue="1.00E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="LJF29_RS21015"
                     /protein_end="187"
                     /protein_start="176"
                     /score="14.9"
                     /tool="antismash"
                     /translation="HYKDFAVWQNE"
     CDS_motif       complement(1585..1650)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21015_0002"
                     /evalue="1.20E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="LJF29_RS21015"
                     /protein_end="166"
                     /protein_start="144"
                     /score="30.7"
                     /tool="antismash"
                     /translation="MHHIIADGVSRGIFVKELALLY"
     CDS_motif       complement(1846..1956)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21015_0001"
                     /evalue="4.10E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="LJF29_RS21015"
                     /protein_end="79"
                     /protein_start="42"
                     /score="51.3"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGELHISRLRESLNQLVNRHESLRTSF"
     gene            complement(<2095..>5088)
                     /gene="srfAB"
                     /locus_tag="LJF29_RS21020"
                     /old_locus_tag="LJF29_21015"
                     /pseudo=""
     CDS             complement(<2095..5088)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (1-264). E-value:
                     3.9e-95. Score: 310.2. Matches aSDomain:
                     nrpspksdomains_LJF29_RS21020_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (428-831). E-value:
                     1.1e-114. Score: 375.1. Matches aSDomain:
                     nrpspksdomains_LJF29_RS21020_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (937-997). E-value: 4.1e-23. Score:
                     73.4. Matches aSDomain: nrpspksdomains_LJF29_RS21020_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="LJF29_RS21020"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="LJF29_21015"
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 1.6e-85, bitscore:
                     275.2, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.6e-123, bitscore:
                     400.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.5e-15, bitscore:
                     46.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASTSYNVPAVLLLEGEVNKDRLEHAIQQLIDRHEILRTSFDMID
                     GEVVQTVHKNISFQLEAAKGREEDAEELIKSFVQPFELNRAPLVRSKLVQLEEERHLLL
                     IDMHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWQKEHADHQKDEAYWLDT
                     FKGELPVLDLPADFPRPAERSFSGERVMFGLDKQITAQIKSLLSETDTTMYMFLLAAFH
                     VLLSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTAPAGDKTFAQFLNEVKTV
                     SLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNMQNMEIPSLKLGDLKISSYSMLH
                     HVAKFDLSLEAVEREEEIGLSFDYATVLFKDETIRRWSSHFVNLIKAAAVNPNVRLSDL
                     EILSSAETAALLEEKKQTTHSGEDTFVSLFEKQVQQTPDDPAVISGGNQLTYRELDEQA
                     NQLAHHLRAHGAGSEDIVAVVMDRSADLMTAILGVMKAGAAFLPIDPDTPEERIRYSLE
                     DSGARFTVVNERNMTAIGQYQGTTINIDKADWRNENKERPELMSGPQNLAYIIYTSGTT
                     GKPKGVQIEHRNLTNYVSWFSKEAGLTKTDKSVLLSSYAFDLGYTSIFPVLQAGGELHI
                     VPKETYTAPDQIGRYIKEHGITYIKLTPSLFHTMVNTASFTKEPHFESLRLIVLGGEKI
                     IPADVLAFSKVYRHTEFINHYGPTEATIGAIAGRVDLTEPGTFAKRPTIGRPIANTGAL
                     VLNESLKLVPPGASGQLYITGQGLARGYLSRPQLTSEKFVENPYAPGSLMYKTGDVVRR
                     LADGTVEFIGRADDQVKIRGYRIEPKEIETVMLSLSDIREAVVLAVSEGGLQELCAYYT
                     ADQAIEKAELRKQLSQILPSHMIPAFFVQVDVIPLTANGKTDRNALPKPNAAQSGYEAS
                     AAPETALEESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELEKTVPLKA
                     LFEQPTVRQ"
     aSDomain        complement(2098..2277)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJF29_RS21020_PCP.1"
                     /evalue="4.10E-23"
                     /label="LJF29_RS21020_PCP.1"
                     /locus_tag="LJF29_RS21020"
                     /protein_end="997"
                     /protein_start="937"
                     /score="73.4"
                     /tool="antismash"
                     /translation="EESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELE
                     KTVPLKALFEQPTVR"
     aSModule        complement(2098..5085)
                     /complete
                     /domains="nrpspksdomains_LJF29_RS21020_Condensation_LCL.1"
                     /domains="nrpspksdomains_LJF29_RS21020_AMP-binding.1"
                     /domains="nrpspksdomains_LJF29_RS21020_PCP.1"
                     /locus_tags="LJF29_RS21020"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(2566..2631)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21020_0009"
                     /evalue="3.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="LJF29_RS21020"
                     /protein_end="841"
                     /protein_start="819"
                     /score="38.2"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIEPKEIE"
     aSDomain        complement(2596..3804)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJF29_RS21020_AMP-binding.1"
                     /evalue="1.10E-114"
                     /label="LJF29_RS21020_AMP-binding.1"
                     /locus_tag="LJF29_RS21020"
                     /protein_end="831"
                     /protein_start="428"
                     /score="375.1"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FEKQVQQTPDDPAVISGGNQLTYRELDEQANQLAHHLRAHGAGSE
                     DIVAVVMDRSADLMTAILGVMKAGAAFLPIDPDTPEERIRYSLEDSGARFTVVNERNMT
                     AIGQYQGTTINIDKADWRNENKERPELMSGPQNLAYIIYTSGTTGKPKGVQIEHRNLTN
                     YVSWFSKEAGLTKTDKSVLLSSYAFDLGYTSIFPVLQAGGELHIVPKETYTAPDQIGRY
                     IKEHGITYIKLTPSLFHTMVNTASFTKEPHFESLRLIVLGGEKIIPADVLAFSKVYRHT
                     EFINHYGPTEATIGAIAGRVDLTEPGTFAKRPTIGRPIANTGALVLNESLKLVPPGASG
                     QLYITGQGLARGYLSRPQLTSEKFVENPYAPGSLMYKTGDVVRRLADGTVEFIGRADDQ
                     VKIR"
     CDS_motif       complement(2713..2802)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21020_0008"
                     /evalue="2.10E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="LJF29_RS21020"
                     /protein_end="792"
                     /protein_start="762"
                     /score="42.4"
                     /tool="antismash"
                     /translation="PPGASGQLYITGQGLARGYLSRPQLTSEKF"
     CDS_motif       complement(3331..3390)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21020_0007"
                     /evalue="1.40E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="LJF29_RS21020"
                     /protein_end="586"
                     /protein_start="566"
                     /score="33.0"
                     /tool="antismash"
                     /translation="AYIIYTSGTTGKPKGVQIEH"
     CDS_motif       complement(3577..3618)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21020_0006"
                     /evalue="3.40E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="LJF29_RS21020"
                     /protein_end="504"
                     /protein_start="490"
                     /score="17.0"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     CDS_motif       complement(4135..4248)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21020_0005"
                     /evalue="1.60E-16"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="LJF29_RS21020"
                     /protein_end="318"
                     /protein_start="280"
                     /score="52.6"
                     /tool="antismash"
                     /translation="VSLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNM"
     aSDomain        complement(4297..5085)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJF29_RS21020_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.90E-95"
                     /label="LJF29_RS21020_Condensation_LCL.1"
                     /locus_tag="LJF29_RS21020"
                     /protein_end="264"
                     /protein_start="1"
                     /score="310.2"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="ASTSYNVPAVLLLEGEVNKDRLEHAIQQLIDRHEILRTSFDMIDG
                     EVVQTVHKNISFQLEAAKGREEDAEELIKSFVQPFELNRAPLVRSKLVQLEEERHLLLI
                     DMHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWQKEHADHQKDEAYWLDTF
                     KGELPVLDLPADFPRPAERSFSGERVMFGLDKQITAQIKSLLSETDTTMYMFLLAAFHV
                     LLSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTA"
     CDS_motif       complement(4303..4392)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21020_0004"
                     /evalue="9.20E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="LJF29_RS21020"
                     /protein_end="262"
                     /protein_start="232"
                     /score="50.1"
                     /tool="antismash"
                     /translation="DIIVGSPTAGRTHPDLQGVPGMFVNTVALR"
     CDS_motif       complement(4639..4674)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21020_0003"
                     /evalue="9.30E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="LJF29_RS21020"
                     /protein_end="150"
                     /protein_start="138"
                     /score="15.0"
                     /tool="antismash"
                     /translation="HYKDYAVWQKEH"
     CDS_motif       complement(4705..4770)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21020_0002"
                     /evalue="8.40E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="LJF29_RS21020"
                     /protein_end="128"
                     /protein_start="106"
                     /score="28.0"
                     /tool="antismash"
                     /translation="MHHIITDGSSTGILIGDLAKIY"
     CDS_motif       complement(4966..5079)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21020_0001"
                     /evalue="1.10E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="LJF29_RS21020"
                     /protein_end="41"
                     /protein_start="3"
                     /score="56.4"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGEVNKDRLEHAIQQLIDRHEILRTSF"
     gene            complement(5034..5174)
                     /gene="comS"
                     /locus_tag="LJF29_RS21025"
                     /old_locus_tag="LJF29_21020"
     CDS             complement(5034..5174)
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388232.1"
                     /locus_tag="LJF29_RS21025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJF29_21020"
                     /product="competence protein ComS"
                     /protein_id="WP_229150297.1"
                     /transl_table=11
                     /translation="MNRSGKHLISSIILYPRPSGECTSSISLDKQAQATTSLLYFCWRE
                     K"
     aSDomain        complement(<5218..5400)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJF29_RS21030_PCP.1"
                     /evalue="1.30E-19"
                     /label="LJF29_RS21030_PCP.1"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="1031"
                     /protein_start="970"
                     /score="62.1"
                     /tool="antismash"
                     /translation="ETKLAEIWEGILGVKASVTDNFFMIGGHSLKAMMMTAKIQEHFHK
                     EVPIKVLFEKPTIQEL"
     aSModule        complement(<5218..8292)
                     /complete
                     /domains="nrpspksdomains_LJF29_RS21030_Condensation_DCL.1"
                     /domains="nrpspksdomains_LJF29_RS21030_AMP-binding.1"
                     /domains="nrpspksdomains_LJF29_RS21030_PCP.1"
                     /locus_tags="LJF29_RS21030"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     gene            complement(<5218..8310)
                     /gene="srfAB"
                     /locus_tag="LJF29_RS21030"
                     /old_locus_tag="LJF29_21025"
                     /pseudo=""
     CDS             complement(<5218..8310)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-29). E-value: 1.4e-14.
                     Score: 45.0. Matches aSDomain:
                     nrpspksdomains_LJF29_RS21030_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (6-306). E-value:
                     4.9e-115. Score: 375.7. Matches aSDomain:
                     nrpspksdomains_LJF29_RS21030_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (470-864). E-value:
                     8.9e-113. Score: 368.9. Matches aSDomain:
                     nrpspksdomains_LJF29_RS21030_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1031). E-value: 1.3e-19. Score:
                     62.1. Matches aSDomain: nrpspksdomains_LJF29_RS21030_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="LJF29_RS21030"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="LJF29_21025"
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 1.2e-90, bitscore:
                     292.0, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 8.5e-119, bitscore:
                     385.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 8.2e-15, bitscore:
                     43.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIQGVFD
                     LEIFEKSVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISHADEDRQKDHI
                     ERYKQDVQRQGFNLAKDILFKVAVFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNY
                     EALRAGRAPANGQGKPYSDYIKWLGKQDNEEAEGYWSERLANFEQPSVLPGRLPEKKDE
                     YVNREYSFTWDEELVARIQQTANRHQVTGPNLFQAIWGIVLSKYNFTNDVVFGTVVSGR
                     PSEINGIETMAGLFINTIPVRVKVDRQSTFADIFTAVQQHAVEAERYDYVPLYEIQKRS
                     ALDSSLLNHLVAFENYPLDQELENGSMEDRLGFSIKVESAFEQTSFDFNLIVYPGKTWT
                     VKIKYNGAAFDSAFIERTAGHLTRMMTAAVERPDAFVREHGLVGEEEQRQIVETFNSTS
                     TELPERMAVHQVFEEQAKRTPASTALVYEGAELTYRELNEAANRLARKLIGQGLQQGET
                     AAIMNDRSAETVAAILAVLKAGAAYVPLDPALPEDRLRFMAEDSSIRIALAGEAYTKQA
                     NQLQVPVLPLEGVFEENETADNLNLPADPTDLAYIMYTSGSTGKPKGVMIEHKSILRLV
                     KNAGYVPVHEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDATRFSAFLRE
                     QKITTMWLTSPLFNQLAAKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNGYG
                     PTENTTFSTSFLIDREYSGSIPIGKPIGNSTAYIMDEQQRLQPIGAPGELCVGGSGVAR
                     GYVNLPELTEKQFLEDPFRPGEKMYRTGDLAKWLPDGNIEFLGRIDNQVKVRGFRIELG
                     EIENKLNMAEHVTEAAVIIRKNKADENEICAYFTADREVSVSGLRKTLSQSLPDYMVPA
                     HLIQMDSLPLTPNGKINKKELPAPQSDAVQSEYAAPKTESETKLAEIWEGILGVKASVT
                     DNFFMIGGHSLKAMMMTAKIQEHFHKEVPIKVLFEKPTIQEL"
     CDS_motif       complement(5689..5754)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21030_0010"
                     /evalue="1.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="874"
                     /protein_start="852"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        complement(5719..6900)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJF29_RS21030_AMP-binding.1"
                     /evalue="8.90E-113"
                     /label="LJF29_RS21030_AMP-binding.1"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="864"
                     /protein_start="470"
                     /score="368.9"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQAKRTPASTALVYEGAELTYRELNEAANRLARKLIGQGLQQG
                     ETAAIMNDRSAETVAAILAVLKAGAAYVPLDPALPEDRLRFMAEDSSIRIALAGEAYTK
                     QANQLQVPVLPLEGVFEENETADNLNLPADPTDLAYIMYTSGSTGKPKGVMIEHKSILR
                     LVKNAGYVPVHEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDATRFSAFL
                     REQKITTMWLTSPLFNQLAAKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNG
                     YGPTENTTFSTSFLIDREYSGSIPIGKPIGNSTAYIMDEQQRLQPIGAPGELCVGGSGV
                     ARGYVNLPELTEKQFLEDPFRPGEKMYRTGDLAKWLPDGNIEFLGRIDNQVKVR"
     CDS_motif       complement(5836..5925)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21030_0009"
                     /evalue="2.30E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="825"
                     /protein_start="795"
                     /score="48.7"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGSGVARGYVNLPELTEKQF"
     CDS_motif       complement(6427..6486)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21030_0008"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="628"
                     /protein_start="608"
                     /score="35.6"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       complement(6673..6714)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21030_0007"
                     /evalue="1.60E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="546"
                     /protein_start="532"
                     /score="21.1"
                     /tool="antismash"
                     /translation="LAVLKAGAAYVPLD"
     CDS_motif       complement(7240..7356)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21030_0006"
                     /evalue="3.20E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="357"
                     /protein_start="318"
                     /score="55.0"
                     /tool="antismash"
                     /translation="QQHAVEAERYDYVPLYEIQKRSALDSSLLNHLVAFENYP"
     aSDomain        complement(7393..8292)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJF29_RS21030_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="4.90E-115"
                     /label="LJF29_RS21030_Condensation_DCL.1"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="306"
                     /protein_start="6"
                     /score="375.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIQGVFDLEIFEK
                     SVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISHADEDRQKDHIERYKQD
                     VQRQGFNLAKDILFKVAVFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAG
                     RAPANGQGKPYSDYIKWLGKQDNEEAEGYWSERLANFEQPSVLPGRLPEKKDEYVNREY
                     SFTWDEELVARIQQTANRHQVTGPNLFQAIWGIVLSKYNFTNDVVFGTVVSGRPSEING
                     IETMAGLFINTIPVRVKVD"
     CDS_motif       complement(7405..7500)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21030_0005"
                     /evalue="7.50E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="302"
                     /protein_start="270"
                     /score="63.4"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       complement(7738..7776)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21030_0004"
                     /evalue="1.20E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="191"
                     /protein_start="178"
                     /score="11.5"
                     /tool="antismash"
                     /translation="PYSDYIKWLGKQD"
     CDS_motif       complement(7822..7887)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21030_0003"
                     /evalue="1.20E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="163"
                     /protein_start="141"
                     /score="37.0"
                     /tool="antismash"
                     /translation="NHHIMMDGWSMGVLMKSLFQNY"
     CDS_motif       complement(8104..8220)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21030_0002"
                     /evalue="3.00E-13"
                     /label="C2_DCL_024-062"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="69"
                     /protein_start="30"
                     /score="42.0"
                     /tool="antismash"
                     /translation="SYFTQLELGIQGVFDLEIFEKSVNELIRSYDILRTVFVH"
     aSDomain        complement(8224..8310)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJF29_RS21030_NRPS-COM_Nterm.1"
                     /evalue="1.40E-14"
                     /label="LJF29_RS21030_NRPS-COM_Nterm.1"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="29"
                     /protein_start="0"
                     /score="45.0"
                     /tool="antismash"
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHS"
     CDS_motif       complement(8245..8283)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21030_0001"
                     /evalue="2.40E-05"
                     /label="C1_dual_004-017"
                     /locus_tag="LJF29_RS21030"
                     /protein_end="22"
                     /protein_start="9"
                     /score="16.9"
                     /tool="antismash"
                     /translation="YALTPMQEGMLYH"
     CDS             complement(8323..>9850)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Epimerization (43-346). E-value:
                     1.5e-172. Score: 567.2. Matches aSDomain:
                     nrpspksdomains_LJF29_RS21035_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (351-505). E-value: 4e-55.
                     Score: 177.8. Matches aSDomain:
                     nrpspksdomains_LJF29_RS21035_TIGR01720.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="LJF29_RS21035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJF29_21030"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_229150295.1"
                     /sec_met_domain="Condensation (E-value: 6e-97, bitscore:
                     312.7, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASRLNQHGWKLEMKDLFQHPTIEELTQYVERAEGKQADQGPVEG
                     EVILTPIQRWFFEKNFTNKHHWNQSVMLHAAKGFDPERVEKTLQALIEHHDALRMVYRE
                     EQGEIVQFNKGLDAVSVHLEITKLEGQAIDHEDRIEREAERLQSSINLKEGGLLKAGLF
                     QAEDGDHLLLVIHHLVVDGVSWRILLEDFAAVYTQLEQGNEPVLPQKTHSFAEYAERLQ
                     DFANSKAFLKEKEYWKQLEEQTVPVKLPKDRESSDQRMKHTKTIGFSLTAEETEQLTTK
                     VHEAYHTEMNDILLTAFGLAMKEWTGQEQVSVHLEGHGREDIVEDLAISRTVGWFTSMF
                     PMVLDMKHADDLAYQLKQMKEDIRHIPNKGVGYGILRYLTAPEHKEDVAFSIQPDVSFN
                     YLGQFDEMSDAGLFTRSELPSGQSLSPDTEKPNALDVVGYIENGKLTMSLAYHSLEFHE
                     KTIQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLGELDKLMEIF"
     gene            complement(8323..>9854)
                     /locus_tag="LJF29_RS21035"
                     /old_locus_tag="LJF29_21030"
     aSDomain        complement(8335..8796)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJF29_RS21035_TIGR01720.1"
                     /evalue="4.00E-55"
                     /label="LJF29_RS21035_TIGR01720.1"
                     /locus_tag="LJF29_RS21035"
                     /protein_end="505"
                     /protein_start="351"
                     /score="177.8"
                     /tool="antismash"
                     /translation="LAYQLKQMKEDIRHIPNKGVGYGILRYLTAPEHKEDVAFSIQPDV
                     SFNYLGQFDEMSDAGLFTRSELPSGQSLSPDTEKPNALDVVGYIENGKLTMSLAYHSLE
                     FHEKTIQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLGELDKL"
     aSModule        complement(8335..9720)
                     /domains="nrpspksdomains_LJF29_RS21035_Epimerization.1"
                     /domains="nrpspksdomains_LJF29_RS21035_TIGR01720.1"
                     /incomplete
                     /locus_tags="LJF29_RS21035"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(8713..8772)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21035_0005"
                     /evalue="8.50E-10"
                     /label="NRPS-E6"
                     /locus_tag="LJF29_RS21035"
                     /protein_end="379"
                     /protein_start="359"
                     /score="30.8"
                     /tool="antismash"
                     /translation="KEDIRHIPNKGVGYGILRYL"
     aSDomain        complement(8812..9720)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LJF29_RS21035_Epimerization.1"
                     /evalue="1.50E-172"
                     /label="LJF29_RS21035_Epimerization.1"
                     /locus_tag="LJF29_RS21035"
                     /protein_end="346"
                     /protein_start="43"
                     /score="567.2"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="EGEVILTPIQRWFFEKNFTNKHHWNQSVMLHAAKGFDPERVEKTL
                     QALIEHHDALRMVYREEQGEIVQFNKGLDAVSVHLEITKLEGQAIDHEDRIEREAERLQ
                     SSINLKEGGLLKAGLFQAEDGDHLLLVIHHLVVDGVSWRILLEDFAAVYTQLEQGNEPV
                     LPQKTHSFAEYAERLQDFANSKAFLKEKEYWKQLEEQTVPVKLPKDRESSDQRMKHTKT
                     IGFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWTGQEQVSVHLEGHGREDIV
                     EDLAISRTVGWFTSMFPMVLDM"
     CDS_motif       complement(8818..8865)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21035_0004"
                     /evalue="2.30E-08"
                     /label="NRPS-E5"
                     /locus_tag="LJF29_RS21035"
                     /protein_end="344"
                     /protein_start="328"
                     /score="26.1"
                     /tool="antismash"
                     /translation="ISRTVGWFTSMFPMVL"
     CDS_motif       complement(9274..9339)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21035_0003"
                     /evalue="1.70E-12"
                     /label="NRPS-E2"
                     /locus_tag="LJF29_RS21035"
                     /protein_end="192"
                     /protein_start="170"
                     /score="39.4"
                     /tool="antismash"
                     /translation="LLLVIHHLVVDGVSWRILLEDF"
     CDS_motif       complement(9541..9615)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21035_0002"
                     /evalue="1.80E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="LJF29_RS21035"
                     /protein_end="103"
                     /protein_start="78"
                     /score="20.4"
                     /tool="antismash"
                     /translation="FDPERVEKTLQALIEHHDALRMVYR"
     CDS_motif       complement(9676..9705)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LJF29_RS21035_0001"
                     /evalue="3.00E-05"
                     /label="NRPS-E1"
                     /locus_tag="LJF29_RS21035"
                     /protein_end="58"
                     /protein_start="48"
                     /score="16.6"
                     /tool="antismash"
                     /translation="LTPIQRWFFE"
ORIGIN
        1 gcgggtgata tcaaggcgga tcgcttcatc aatgacgtga agctcagcgc cgatcgtcca
       61 ggtcgggaac atctcccaaa tggaagcgtc gaacccgaag cccgcgtact tcgcactgcg
      121 gtcctccgcc gtcatgccga acgcgtcatg atgccagaag cacaggttca caagcgcatg
      181 gtgctcgatc ataacaccct tcggctgtcc ggtcgttcct gacgtgtaaa tcatataagc
      241 aaggtgatcc gcggttgtgt cggtctgcgg atcttcctca tcgttttcat caagcggcag
      301 actcaggatc gtccggttgc cgtcaagaag aatgacctcg cccgtgtagc tgtccggcac
      361 tgtgatgcct tcctgtttca agagaagcgt cgcaccgctg tcctgtagga tatattcaat
      421 ccgctgatcc ggatagtcag ggtcaatcgg cacaaatgcc gcgcccgcct tcaggacacc
      481 aagcaccgcg gcggacattt caagcgacgg cttcgtcaga atgccgacgc gctcatccgc
      541 gctgatgcca cagtcgatca aaatccgcgc cagacggttc gccttcgcat tcagctcgcc
      601 gtacgtccat gactgcccgt tatacgtgac agccgggcgg tctttatggc gctgaacggt
      661 ttcttcaaac agctgatgaa ccgttttgtc tgtcggcaca ggcagcgttt ttcctttcca
      721 tgcctccaga agcgtttgtt tttctgtctc gttgacgagc gacaggctgg aaagcggctg
      781 gtttggattt tccgcaacag cctgcaataa gcggaggaca tattcgctcc atttctcagc
      841 tgtttctctt gagaatagat ccgtcgcata ttccagctgc agaccgatgc cgttttcgtc
      901 agtgaaaccg cctaatgtca gatcaaactt cgatgttccc tgatgggcct cataaggtga
      961 aatcgtgaga tcttgcagct caagctgttc tttatcaggg ttttctgtcg taaccatcac
     1021 attaaacaat ggatttcggc tcatatcccg aggaaggtct aactggttga ccagactttc
     1081 aaacggatac tcctgatgct caagcgctga cagattcgtc tcctttgctt gctccagaag
     1141 ctctgcaaat gtctgctttt ctcctgcttc cattctcagt gcaagcgtat tgacgaacat
     1201 ccccggcatg ttttgcacgt cggcattcgt gcggcctgct gtaaccgagc cgatcacgat
     1261 atcccgctgt ccggagattt tgcttaaaaa gacatggaag acggtatgaa gcaccatatg
     1321 caaagtcgtt cccgtttctg cgagcagttt ttcaatcgcc tttgccgtcc cgcttcccgc
     1381 gcggaaacgg acagtatccc cttcaaagct ttgaacaggc ggtcgggtgt aatcaagcgg
     1441 aaggtcaagc tccggcagtt cgcctgagag aactgatagc cagtattgct catgctcctt
     1501 catccgttct ttctgtccgg cttcattttg ccaaacagcg aaatccttat aatgaagctt
     1561 cggctctgga agctgttcgc ctttataaag caatgccaat tctttaacga aaatacctct
     1621 tgatacacca tccgcgataa tgtgatgcat atccaataga agcagatgtt tttggtctgc
     1681 cattcggagt aatgccgcgc gaatgagcgg agcgttgctt aagtcgaatg gcctgataaa
     1741 ttccttaatc ttccgttccg cctcgccctc ttctgcatca aacacatgaa gatcaattga
     1801 tgcctcctca acaatgcgct ggacaggctc tccgtctgct tccgtaaatg acgtgcgcaa
     1861 tgattcatga cggtttacga gctggttaag tgattctctc aggcgcgaaa tgtgaagctc
     1921 gccttccatc agaagaaccg atggcatatt atagctgatc gtctgccggt caagctgatt
     1981 gagcacgtac atccgtttct gtgcggatga caatggatac acatcttgtt tgtcagccgg
     2041 ctttaacatg tgatagccgc ctgatgctgc cgattcctcc atgtaagccg ccagctggcg
     2101 tacagtcggc tgctcgaaca gcgctttaag cggcacggtt ttctccaatt ccgcctgtat
     2161 attggcgatg agcatcattc cttttagtga atggccgcct aaatcgaaga aattgtcatc
     2221 gattccgatc gcttcaatgc cgagtgtttt ctgccagatg cggcaaaggc tttcttcaag
     2281 tgctgtttcc ggagcagctg acgcctcata tccggattgc gctgcgttcg gcttcggcaa
     2341 tgcgtttctg tcggtcttcc cgtttgccgt gagtgggatg acatctacct gcacgaagaa
     2401 ggcagggatc atatgagacg gcagtatttg tgaaagctgt ttccggagct ctgctttttc
     2461 aatggcttga tccgccgtat aatacgcaca aagctcttga agcccgccct cagaaaccgc
     2521 aagcactacc gcttctcgaa tgtcgctgag gctaagcatg accgtttcaa tttctttcgg
     2581 ctctatgcgg tagccacgga ttttcacctg atcatcagcc cggccgataa attcaaccgt
     2641 accgtccgcc agtctccgga cgacatctcc ggtcttgtac atcaggctcc ccggcgcata
     2701 cgggttttca acgaatttct cggatgtgag ctgaggacgg ctgagatatc ctctggcaag
     2761 cccctgtcct gtgatatata actgaccgct cgctcccgga ggcacaagct tcaatgactc
     2821 atttaagaca agtgcgccgg tattggcaat cggccgtccg attgtcggcc gtttcgcgaa
     2881 tgtgccaggt tcagtcaggt caacccgtcc ggcaatggcg ccgattgttg cttcggtcgg
     2941 accgtagtga ttgataaatt cggtatgccg atatactttg ctgaacgcta gaacatcagc
     3001 agggataatc ttttctcctc ccaacacaat caagcgcagg gattcaaaat gcggctcctt
     3061 tgtaaagctg gccgtgttca ccatcgtatg gaacagggac ggtgtcagct tgatatacgt
     3121 gatgccgtgc tccttaatat aacggccaat ctgatcgggc gccgtatagg tttctttcgg
     3181 cacaatgtga agctcaccgc ctgcttgaag cacagggaaa atgcttgtat aacccaagtc
     3241 gaaggcgtaa gacgaaagca agacagactt atccgttttt gtcaggcccg cctctttgct
     3301 aaaccaagaa acatagtttg tcagattgcg atgctcaatc tgcacaccct tcggcttccc
     3361 ggtcgtgccg gatgtgtaaa tgatatatgc aagattctga ggcccagaca tcaattccgg
     3421 acgctccttg ttttcattcc gccaatccgc tttatcgata ttgatcgtgg ttccttgata
     3481 ttgccctatg gcagtcatat ttctttcatt cacgactgta aatctcgcac cgctgtcttc
     3541 taatgaatag cggattcgtt cctcaggcgt atccggatca atcggaagaa aagcggctcc
     3601 cgccttcatg accccaagaa tagctgtcat taggtcagct gaacgatcca tgacaacggc
     3661 tacgatgtct tcacttcctg ccccgtgcgc gcgcaaatga tgcgccagct ggttcgcctg
     3721 ttcatcaagc tcacgatagg tcagctggtt tccgccagaa atcacagccg gatcatcagg
     3781 tgtttgctgc acctgttttt cgaaaagtga tacaaacgtg tcttcgccag aatgagtggt
     3841 ttgttttttc tcttcaagca aagctgccgt ttcagccgat gaaagaatct ccaaatcaga
     3901 cagccgtaca ttcgggttaa ccgcagctgc tttgatcaaa ttgacaaagt ggctgctcca
     3961 gcggcggatt gtctcgtcct taaataagac agttgcgtag tcaaagctta aaccgatttc
     4021 ttcttcacgt tcgacagctt ccaaggacag atcaaatttc gccacgtgat gtagcataga
     4081 ataggaagaa atcttcaaat ctcctaattt caaagaagga atctccatgt tctgcatgtt
     4141 gaacataacg ctgaacagcg ggctacggct tgtgtccctt gtgagcggca gcttttcaat
     4201 caattcctca agcggatagc tttggtgctc gaatgcttga agactgaccg ttttgacttc
     4261 gtttaggaat tgcgcaaagg ttttgtcccc cgcaggcgct gtccgtaatg ccaccgtgtt
     4321 gacaaacata cccggcacac cttgcaaatc aggatgtgtt ctgcccgcag tcggtgagcc
     4381 gacgatgatg tcatcctgtg acgcatactt agagagcaat acatgaaatg cggctaaaag
     4441 gaacatgtac attgttgtgt ctgtttctga cagcagcgat ttgatttgag ccgtgatttg
     4501 tttatcaagc ccgaacatga cgcgctcccc ggaaaagctc cgttctgcag gccgcgggaa
     4561 atccgccgga aggtcaagaa ccggcagctc gcctttaaac gtatccagcc agtatgcttc
     4621 atctttttga tgatccgcat gttctttttg ccagacagcg taatccttat agtgaatctg
     4681 cgggagctcc agatctgcgc cttgatagat tttggcgaga tcgccgatga gaatgccagt
     4741 cgagctgccg tccgtaatga tatgatgcat atcaatgagc agcagatggc gttcttcttc
     4801 aagctggaca agcttcgaac ggacgagcgg cgcgcggttt aactcaaacg gctgaacaaa
     4861 cgatttgatc agctcttccg cgtcttcttc ccgtcccttg gcagcctcca gctggaacga
     4921 tatgttttta tgaacggtct gtacgacttc tccgtctatc atgtcaaacg acgtacggag
     4981 aatttcgtgc cgatcaatta attgctgaat cgcatgttca agccggtctt tgtttacttc
     5041 tccctccagc aaaagtacag cagggacgtt gtagcttgtg cttgcttgtc caagctgatt
     5101 gaggatgtac attctccgct gggccgggga tacaggataa tgctgctgat aaggtgcttg
     5161 cctgatcggt tcaaacgtct gctcctcctt gctttcgctc tcttccaaat acagtgccag
     5221 ttcttgaatg gtcggctttt caaaaagcac tttaatcggt acttccttgt ggaaatgctc
     5281 ttgaattttc gccgtcatca tcatcgcttt cagagaatgt ccgccgatca taaagaagtt
     5341 atctgtaacg cttgctttaa cgccaagtat cccttcccaa atctcagcta atttcgtttc
     5401 actctctgtt ttgggtgctg catattcaga ctgcacagcg tctgattgag gcgcaggcag
     5461 ttctttttta ttgatttttc cgtttggtgt aagcggcagg ctgtccatct ggatcaaatg
     5521 ggcaggaacc atatagtccg gcaatgattg agacagcgtt tttctcagcc cgctcacaga
     5581 cacttcgcga tccgccgtaa agtacgcgca gatttcattt tcatccgctt tgttcttgcg
     5641 aatgatcacg gcagcctctg tcacatgttc ggccatgttc aatttgtttt cgatctcccc
     5701 aagctcaatc cggaacccgc gcaccttcac ctggttatca attcttccta aaaactcgat
     5761 attcccgtcc ggaagccatt tcgccaagtc gccagttcga tacatttttt cacctggtct
     5821 gaacggatct tcgagaaatt gcttctccgt cagctctggg aggttgacgt agccccgtgc
     5881 cacgccgctt cccccgacac acagttcacc gggcgctccg atcggctgca aacgctgctg
     5941 ctcatccatg atgtatgcag tggaatttcc gattggcttc ccgattggaa ttgaaccgct
     6001 gtactcgcga tcaatcaaaa agcttgtaga aaatgtcgta ttttctgtcg ggccgtaccc
     6061 gttccacaga gaaagcgacg gcgatgcctg tttcacttta cttacaatat gcggcacgag
     6121 cgcgtctccg ccgatgatta aatggcgcag tgtgccaaac atgccggcat cctttgcagc
     6181 aagctggttg aataatggcg atgtcagcca catcgtcgta attttctgct cacgcaggaa
     6241 cgctgaaaac cgtgtcgcgt caagcagtgt ctcttttttc accggataaa gcgccgcgcc
     6301 gttcagcagc gcaccaaaga cctcaaatgt gccggcatca aagctgacag ctcccgtttg
     6361 cgccatgcgg tcttcttcat gaacaggaac gtatcctgca tttttgacga gacgcagaat
     6421 gcttttgtgt tcgatcatga cgcctttcgg ttttccggtc gaacctgacg tgtacatgat
     6481 gtaagccaga tcagtcggat cggcgggcag attcagattg tcagccgttt cattctcttc
     6541 aaagacgcct tccaggggga gaaccggcac ttgcagctga tttgcctgtt ttgtataggc
     6601 ctctccagct aaagcgatcc gaatcgagct gtcctccgcc atgaaacgga gccgatcctc
     6661 cggaagtgcg ggatcaagcg gcacatatgc ggcgccggct tttaaaacag ccagtatggc
     6721 ggcaacagtc tctgccgaac ggtcattcat aatggctgct gtttcaccct gttgaagacc
     6781 ttgcccgata agctttctcg ccagacggtt cgccgcttcg ttcagctcgc ggtacgtcag
     6841 ctcggctcct tcatatacga gggcagtact tgccggtgtc cgctttgctt gttcttcaaa
     6901 tacttgatga acagccattc tttcagggag ttctgttgaa gtgctgttaa aggtctccac
     6961 aatttgccgc tgctcttctt ctcccacaag cccatgctca cgcacaaagg cgtccggccg
     7021 ctcgacagcg gctgtcatca tgcgggtgag atgtcctgct gtccgttcaa taaaagcaga
     7081 atcaaatgca gcgccgttat atttgatttt gacggtccag gttttacccg ggtaaacaat
     7141 gaggttgaaa tcaaagcttg tttgttcaaa tgcgctctca accttgatag agaacccgag
     7201 cctgtcttcc atgctgccgt tttcaagctc ttgatcaagc ggataatttt caaaagcgac
     7261 aagatggttc aaaagactgc tgtccaaggc tgaacgtttt tgaatctcgt aaagcggcac
     7321 gtaatcgtag cgctccgcct ccaccgcatg ctgctgaacg gctgtgaaaa tatcagcgaa
     7381 tgtagactgc cgatcaacct ttacacgcac cggaatggta ttgatgaaga gccccgccat
     7441 tgtttcgatt ccgttgattt cagacggccg tcccgatacg accgtgccga agacaacatc
     7501 attcgtaaag ttatatttgc tgaggacgat tccccagatc gcctgaaaca ggttaggccc
     7561 cgtcacttga tggcggttcg ccgtttgctg aatgcgtgca accagttcct catcccatgt
     7621 aaatgagtat tctctgttca catactcgtc tttcttctcc ggcaagcggc ccgggagcac
     7681 gcttggctgt tcaaaatttg ccaagcgttc gctccagtag ccttccgctt cttcattatc
     7741 ctgttttcca agccacttaa tatagtcgga atatggcttg ccctgcccat ttgcaggtgc
     7801 gcggcctgct ctgagcgctt cgtagttttg gaacaggctt ttcatcagca cgcccatgct
     7861 ccagccgtcc atcataatat ggtgattgct ccagacaaga tacagctgat ctgcatcgag
     7921 gcggaaaact gccaccttga acaaaatgtc ttttgccagg ttaaagcctt ggcgctgaac
     7981 gtcttgtttg taacgctcaa tgtggtcttt ctggcgatcc tcgtctgcat gactgatatc
     8041 ttcatagtgc acctttgtct tgcgttccgc taacacaacc tgacgcggct tttgcagctg
     8101 ctggtgcaca aataccgtac ggagaatatc gtatgaccgg atcagttcat tgacgctttt
     8161 ctcaaagatt tcaagatcaa aaacaccttg aatgccaagc tctaattgtg tgaagtaaga
     8221 ggaagaatgc ggatcaagca tcgcatgata cagcattcct tcctgcatcg gtgtaagtgc
     8281 gtacaccttt tgaattgatt ttttgctcat atgccacctc tattagaaaa tttccattaa
     8341 tttatcaagc tcacccagcg tcaaatcgtc gtcgccaagg tcactcggcg ttagttccgt
     8401 gccatcttga gataggcaat gttcaatgat tctaagaaga tgggccttaa agctgtcact
     8461 gaatgtctgt atcgtttttt catgaaattc gagagaatga taggccagtg acatcgtcag
     8521 ttttccattt tcaatatatc cgacaacatc aagcgcgttc ggtttttccg tgtccgggct
     8581 taatgactgt cctgatggca gctctgatct cgtgaataaa cctgcatccg acatttcgtc
     8641 gaactgccct aaatagttaa agctgacatc cggctgaatg gagaacgcca catcttcttt
     8701 atgctccggc gctgtcaaat agcgcaaaat gccgtagccc actcccttgt tcgggatatg
     8761 tctgatgtct tctttcattt gcttcagctg ataagccaaa tcatccgcat gcttcatatc
     8821 cagcaccatc gggaacatac tcgtaaacca gccgactgtc cgggaaatgg ccaggtcttc
     8881 tacgatatct tcacgtccat gcccctctaa atgaacactc acttgctcct gacctgtcca
     8941 ctccttcatc gctaatccaa atgccgtcag caaaatatca ttcatttctg tatgataagc
     9001 ctcatgcacc ttagtggtaa gctgttctgt ctcttcagca gtcagcgaga atccgattgt
     9061 ctttgtatgt ttcattcgct gatcactaga ttcgcggtct ttcggaagct tcaccgggac
     9121 agtctgttct tcaagctgtt tccaatactc tttttctttc agaaaggcct tggaatttgc
     9181 gaagtcttgt aatctctctg catactccgc aaacgaatgg gttttctgcg gaagaaccgg
     9241 ctcgttccct tgctccagct gtgtataaac cgcagcgaaa tcctccagta aaatccgcca
     9301 cgatacaccg tcaaccacta agtggtgaat cacaagcagc aagtggtctc catcttccgc
     9361 ttggaacaag cctgctttta acaagccgcc ttccttcaaa ttgatgctgc tttgcagacg
     9421 ctccgcttct ctctctatgc ggtcttcatg atctatagcc tgtccctcaa gtttggtgat
     9481 ctctaagtga acagagacag catcaagccc tttgttaaat tgaacgatct ccccttgttc
     9541 ctcgcggtag accatgcgaa gcgcgtcatg atgctcaatc agtgcctgca atgtcttttc
     9601 aacccgttca ggatcaaagc ccttcgcggc atgaagcata accgactggt tccagtgatg
     9661 tttgttcgtg aagttctttt caaagaacca gcgctgaatc ggcgtcagga taacttcgcc
     9721 ctccaccggg ccttggtctg cctgtttgcc ttcggcacgc tccacatatt gtgtcagttc
     9781 ttcgatcgtc gggtgctgga agagatcctt catttccagc ttccagccgt gctggttcaa
     9841 gcggctcgcc atct
//
