LOCUS       NZ_CP084541            11944 bp    DNA     linear   CON 20-NOV-2024
DEFINITION  Streptomyces marincola strain SCSIO 64649 chromosome, complete
            genome.
ACCESSION   NZ_CP084541
VERSION     NZ_CP084541.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces marincola
  ORGANISM  Streptomyces marincola
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6629020)
  AUTHORS   Shi,S.
  TITLE     Strepomyces indimicinicus sp. nov., an antibiotic producing
            actinomycetes isolated from the marine environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6629020)
  AUTHORS   Shi,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2021) Key Laboratory of Tropical Marine
            Bio-resources and Ecology, South China Sea Institute of Oceanology,
            Chinese Academy of Sciences, 164 Xingang West Road, guangzhou,
            Guangdong 510301, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. V6.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 444.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020410765.1-RS_2024_11_18
            Annotation Date                   :: 11/18/2024 03:45:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,763
            CDSs (total)                      :: 5,689
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 74
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 21 of 77
            Pseudo Genes (incomplete)         :: 60 of 77
            Pseudo Genes (internal stop)      :: 2 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:57:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2897933
            Orig. end                         :: 2909877
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11944
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="17"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11944
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11944
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6944](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6944
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(188..203)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.14"
                     /tool="antismash"
     gene            225..1343
                     /gene="serC"
                     /locus_tag="LC193_RS12280"
                     /old_locus_tag="LC193_12280"
     CDS             225..1343
                     /EC_number="2.6.1.52"
                     /GO_function="GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_5 (143-331). E-value: 3.9e-18.
                     Score: 57.4. Matches aSDomain:
                     nrpspksdomains_LC193_RS12280_Aminotran_5.1"
                     /codon_start=1
                     /gene="serC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006382893.1"
                     /locus_tag="LC193_RS12280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_12280"
                     /product="phosphoserine transaminase"
                     /protein_id="WP_226074011.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.8e-15, bitscore:
                     55.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADIQIPADIKPADGRFGSGPSKVRTEALDALAATGGSLLGTSHR
                     QAPVKNLVGRVRDGVRSLLSLPADYEVILGNGGSTAFWDVATHGLIEAKSQHLSFGEFS
                     SKFAKAASKAPWLDEPTIITSTPGSLPAPRAQRGADAYGLTHNETSTGVAAPIRRVPGA
                     DEGSLVLVDATSGAGGLPVDITETDVYYFAPQKSFASDGGLWVAAFSPAALERAERIAA
                     SGRHIPEFFSLPTAIDNSRKNQTYNTPALATLFLLAEQLDWLNGQGGLDWAVRRTADSA
                     GRLYGWADKSAVATPFVTDPAQRSQVVGTIDFADGVDAAAIAKVLRANGIVDTEPYRKL
                     GRNQLRVAMYPAVDPADVEALTACVDYVIERL"
     aSDomain        654..1217
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LC193_RS12280_Aminotran_5.1"
                     /evalue="3.90E-18"
                     /label="LC193_RS12280_Aminotran_5.1"
                     /locus_tag="LC193_RS12280"
                     /protein_end="331"
                     /protein_start="143"
                     /score="57.4"
                     /tool="antismash"
                     /translation="LTHNETSTGVAAPIRRVPGADEGSLVLVDATSGAGGLPVDITETD
                     VYYFAPQKSFASDGGLWVAAFSPAALERAERIAASGRHIPEFFSLPTAIDNSRKNQTYN
                     TPALATLFLLAEQLDWLNGQGGLDWAVRRTADSAGRLYGWADKSAVATPFVTDPAQRSQ
                     VVGTIDFADGVDAAAIAKVLRANGI"
     gene            complement(1422..2231)
                     /locus_tag="LC193_RS12285"
                     /old_locus_tag="LC193_12285"
     CDS             complement(1422..2231)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1065: ABC-2 type
                     transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315949.1"
                     /locus_tag="LC193_RS12285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_12285"
                     /product="ABC transporter permease"
                     /protein_id="WP_226078565.1"
                     /transl_table=11
                     /translation="MGQDGPERRVYWAFVDCWTIVKRELVHLVKQPSLIAWQLGFPIVS
                     VLLFVYVFGSAMDVGDGMDYKDYAMPGMFAMTMAFGFMNTAMAVVVEKERGVTDRFRSM
                     PMAPSAVVSGRGVSDVLHAALDLAVMAVIALAIGWRSDGSPLETLYAFALLLLLRFALI
                     WIGVWLGLLVPNMEAAGNLFAVAFPFGMISSVFTPPSMMPDWLGTIAMWNPVSATANAI
                     RDLYGNPLPTGGSWIEENAQLMAVVWPLVITAIFLPLAVRRYQALGR"
     gene            complement(2276..3301)
                     /locus_tag="LC193_RS12290"
                     /old_locus_tag="LC193_12290"
     CDS             complement(2276..3301)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018841712.1"
                     /locus_tag="LC193_RS12290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_12290"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_226074012.1"
                     /transl_table=11
                     /translation="MASASDHSVIAEGLRKRYGDKTALDGLDLAVPRGAVYGLLGPNGA
                     GKTTSVRILSTLLRADSGRALVGGYDVRREPRKVRRRIGMTGQNPAVDEILTARENLRM
                     FGRLFHLDHATARRRTEELLAEFSLLDAADKQVKTYSGGMRRRLDLAAGMILAPEVLFL
                     DEPTTGLDPRGRSEVWDSVRGLTALGTTVLLTTHYLDEADKLSDRIVLIDQGRKITEDT
                     PAGLKRAVGGDRVEVVVSDAADLPAAAEAVARVAGGRRPETDHEGLRVHAPVSDGVAAL
                     TDVAGTLRENGVVCEDIGLRRPTLDEVFLRLTGQSGAREPDRDREGHEGHEGHGKEGGT
                     R"
     misc_feature    3316..3334
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 22.17"
                     /tool="antismash"
     gene            3390..4193
                     /locus_tag="LC193_RS12295"
                     /old_locus_tag="LC193_12295"
     CDS             3390..4193
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431676.1"
                     /locus_tag="LC193_RS12295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_12295"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_226074013.1"
                     /transl_table=11
                     /translation="MTTHSSGSGDIDRSLELMWRPEGRPARGPKPGLTLSAIVGAAVAL
                     ADREGLAALSMRRVAAELGVGTMTLYRYIPGKSELLDLMLDHVVAPVTKPEGGDWRAAL
                     ERIALETFELHLAHPWLLQVNQARPLLGPNGLLGFEYFLAALDGLGLTGRERVNVIMTV
                     DAYVSGLSRHYVMLRQARAESQLTDEEFWGAQGPLIADALNSGRYPHVMSLPEDVFEGT
                     PEEILRFGIDRVLDGVAAFLAALPADRPEERPGWCEEPARDSGSA"
     misc_feature    4291..4306
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.95"
                     /tool="antismash"
     misc_feature    4517..4535
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 24.82"
                     /tool="antismash"
     misc_feature    4518..4534
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.5"
                     /tool="antismash"
     misc_feature    4813..4828
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.7"
                     /tool="antismash"
     gene            4829..4987
                     /locus_tag="LC193_RS12300"
                     /old_locus_tag="LC193_12300"
     CDS             4829..4987
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC193_RS12300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LC193_12300"
                     /product="hypothetical protein"
                     /protein_id="WP_226074014.1"
                     /transl_table=11
                     /translation="MNEELTPDAKGTTDSASGAADNAETHTAGRVPFTVEMMELDNNPI
                     ILQAKSY"
     gene            5001..6944
                     /locus_tag="LC193_RS12305"
                     /old_locus_tag="LC193_12305"
     CDS             5001..6944
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.5"
                     /locus_tag="LC193_RS12305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_12305"
                     /product="YcaO-like family protein"
                     /protein_id="WP_226074015.1"
                     /sec_met_domain="YcaO (E-value: 7.1e-39, bitscore: 132.8,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGPHLVTPLAAQATGPLGTTGFRHRLAPWVVTATRGAGTFVLGP
                     RLSLARIAPGAHDRDEVPVARSIARSMGLDRAVASGWLEPVAPTAPPPPEPPDEPPDEP
                     VTGILVHSDPATAHACTALAERLTAQGAGRWVALAAGQPPPATERPPFVVVAAHRRDAA
                     RDAVPPVRDGRVLHVGEAFEGTYITGPGRGTAPGSDTGDGFAARWAFETRLASLGLTDR
                     PVPLLFRIREDPAAVVRAIMTAMALPPSGAVLVATGRTVRFCTPDAVGSTPLRDVPRTQ
                     AWTFGMARGFGVGPGSVPGSYIATCRTPAGGQDHLEGNSGKGPTEEAATTGAVGEALER
                     YAAYEANWSLPPSRGPARRIDLADLHPYGDAWERRVAAGAAAGPPVAFVDGAGLADGAA
                     VAVPRALVAFPYVGADRPTDGTTTGLAAGPDLAAASLRGLREVLERHSLYGSFAHLRPG
                     YRLDPAASLDRLGLTGAFKGEVWAVHWPHEGYVLPDVHAFHHDPEEGLLVRASGSGLTF
                     DEAMDSALAELCQVHFEGVRARRAGAPAGPAHAAWARRPVVGRARRYLDALPAAAAPPV
                     PYTDPRAQFDHLVSRLADRGTGPVVVRLPLRGPDWTVVRVLVPGATTATAPSDSRGGRR
                     LADAPWTHGIPT"
     gene            7054..8160
                     /locus_tag="LC193_RS12310"
                     /old_locus_tag="LC193_12310"
     CDS             7054..8160
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC193_RS12310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LC193_12310"
                     /product="hypothetical protein"
                     /protein_id="WP_226074017.1"
                     /transl_table=11
                     /translation="MKTLDAFRDLEEALVAADTAAGAPAPGSDARRAHALAEVLADARD
                     IARADPGLLLADLVSDPRGWPPQSWKNYFGNACVTVIRTATLRVDVLYWLQNASTLHKH
                     VSSGAFLALSGRRLHLEYDFSALDSLNAGVTFGQLSATGRSMMREAAVSPILPTMVHEL
                     YWIEKPAVTVSVRCAPAPGSGRSDPDAPRPAPHRPHEFIAPGAGYLPAAFHDTSNVQRW
                     LDGLGMLRRANRELYLGALDTALRLVDSIHLIHVLDELCDNPVDEVSALLARADAARED
                     DELARLAPSVPEFRRRKIFSRAYAKGPDTQLLAALLWAGAEGGELAALLKAEKVADPEE
                     FVRVHGERLSAVDPRIAPYAESARRSLR"
     gene            8157..9398
                     /locus_tag="LC193_RS12315"
                     /old_locus_tag="LC193_12315"
     CDS             8157..9398
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC193_RS12315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LC193_12315"
                     /product="hypothetical protein"
                     /protein_id="WP_226074018.1"
                     /transl_table=11
                     /translation="MNDGWDIASFWEKEPRVLDGPRLDEETLFGLAVAAAARRAGLPDP
                     QDVRVFADDGTVTPARVDAYLPSAGRDGSFARYAERMLAAVGSFALTINSLQQFGWDFW
                     QTLRRATDPVLAAGGWSAGGVDCHLIASVYGTAPTLVHKDTAGVFTYVVRGFKRFYTWP
                     YEVFAGIAGEEATQRQVNLPASIRPEDHLDTATVVEGGPGSVLYWPSDRWHCAMSDGRL
                     AVSLHLAHYQWDDRLALLLRRVRRLAASELGTGRFEGGPVPEAGPGGDRSTDAQVRKVV
                     SRLVDDSALDLDLRLRRLKRRTASNFEVIPPPRPCPDLSATPGPLTVPEGSVARTLTAD
                     GVTYLAVNGHILRITGGAWLDAFLPWLVPGAAHSPEEWANAAAAAGGPSGPECTAFLGE
                     LVKRGALTPGEADA"
     gene            9395..10411
                     /locus_tag="LC193_RS12320"
                     /old_locus_tag="LC193_12320"
     CDS             9395..10411
                     /EC_number="2.1.1.-"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017522.5"
                     /locus_tag="LC193_RS12320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_12320"
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="WP_226074020.1"
                     /transl_table=11
                     /translation="MRLNDGAFVAVKDGQLMLGHVDRPFDLWRADAGVLVLLAEIARGT
                     PAGELPDAMAAVVGAAGESAVPAALAHLRRAHVVADGAGAALRDVNAEVAKVIGAAPDL
                     HADTGFMRAARACEGLTLTSAAAQYALWSAVRHVIAAGVPGALVECGVWRGGSMLLAAL
                     ALLRDGVRDRDLFLFDTFGWSWERTGEHDGFMEPGGTARAPERTPAEEAPAGSAAPGPM
                     DVGVSADEVHARITAAGYPADRVHCVAGLVQDTLPARAPDRIALLRLDTDQYESTLHEL
                     RELYPRVAPGGLVIIDDYGKLAGATRAADAYLAGLDRPALLHRIDTQGRIFVKPDER"
     gene            10417..11448
                     /locus_tag="LC193_RS12325"
                     /old_locus_tag="LC193_12325"
     CDS             10417..11448
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC193_RS12325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LC193_12325"
                     /product="hypothetical protein"
                     /protein_id="WP_226074022.1"
                     /transl_table=11
                     /translation="MVAVIDHAVRPRFSRHVSFALDDHEVRIEDGTYRAAFPAGGVTLP
                     DLDRLAAGVSPDELVAGVRGWLGCGEPDAIAVIERLFSTGLLVDPEAARLDSVPGSYVA
                     CRLVDAFRREFPRVVSGSPLLRDLAAGRDRGLALGFLLETYFVVRAAVWTTDPVFRHPM
                     TPAQREALDDFRASESGHGELLLGGFSAAGFDTDALRRSQAAVETLAYAQSYGAFAWQG
                     VAEFAASLVLPEVPALGADPAAPGVDVLDLIEQRHGVPGSLIARFRAHDAEDVEADHGG
                     LPGILLSEERDLTPAKVERLFVVLRHMMDLYRSHLDAVHRRYAHWDPDAAAGPELPDNA
                     LRF"
ORIGIN
        1 gggggcgtcg ctctcccggc tggaggcccg cgtcgcgctg cccgcgctgt tcgagcgctt
       61 ccccggtctg gcgctggccg tgccggacga ggagttgcgc aatatgccca tcgtcaccca
      121 gaacgacctg gccgaactgc ccgtccggct cggctgacgg tatccggtgt ccacgtcacg
      181 agccggatgt gcggtttcgg cgtccgaggg actacgctcc ggccgtggcc gatatccaga
      241 ttcccgctga catcaagccc gccgacggcc ggttcggctc tggcccctcc aaggtgcgca
      301 ccgaggcact cgacgcactg gccgcgaccg gcggctccct gctcggcacg tcccaccgcc
      361 aggcaccggt caagaacctg gtgggccggg tccgcgacgg cgtgcgctcc ctgctgtcgc
      421 tgcccgccga ctacgaggtg atcctcggca acggcggctc caccgcgttc tgggacgtcg
      481 cgacgcacgg cctcatcgag gcgaagtcgc agcacctgtc gttcggcgag ttctcgtcga
      541 agttcgccaa ggcggcgagc aaggccccgt ggctcgacga gccgaccatc atcacctcga
      601 cgcccggcag cctcccggct ccccgggcgc agcgcggcgc ggacgcctac ggcctcaccc
      661 acaacgagac ctcgaccggc gtcgccgccc ccatccgccg cgtgccgggc gcggacgagg
      721 gctcgctggt cctcgtcgac gccacctcgg gtgccggcgg cctgcccgtc gacatcaccg
      781 agaccgatgt ctactacttc gccccgcaga agtcgttcgc gtccgacggc gggctgtggg
      841 tcgcggcgtt ctccccggcc gccctggagc gcgcggagcg gatcgcggcc tcgggccggc
      901 acatcccgga gttcttctcg ctgccgacgg cgatcgacaa ctcccgcaag aaccagacct
      961 acaacacccc ggcgctcgcc accctgttcc tgctggccga gcagctggac tggctgaacg
     1021 ggcagggcgg cctcgactgg gcggtgcgcc gcaccgccga ctccgccggc cggctctacg
     1081 ggtgggccga caagtcggcg gtcgccacgc cgttcgtcac cgaccccgcg cagcgctccc
     1141 aggtcgtcgg caccatcgac ttcgccgacg gcgtggacgc ggcggccatc gccaaggtcc
     1201 tgcgggccaa cggcatcgtg gacaccgagc cctaccgcaa gctgggccgc aaccagctgc
     1261 gggtcgcgat gtacccggcc gtcgacccgg ccgacgtcga ggcactgacg gcgtgcgtcg
     1321 actacgtcat cgagcgcctc tgagcacacg ggacgcggca cggtgcggcg cccggcggag
     1381 cactccgccg ggcgccgcgc cgcgtacggg cacggccgtg gtcacctgcc gagcgcctgg
     1441 tagcggcgga cggcgagcgg caggaagatc gcggtgatca ccaacggcca gaccaccgcc
     1501 atcagttgcg cgttctcctc gatccagctg ccgccggtgg gcagcgggtt gccgtacagg
     1561 tcgcggatgg cgttcgcagt ggcggagacc gggttccaca tggctatggt gcccagccag
     1621 tccggcatca tcgacggcgg cgtgaacacc gaggagatca tgccgaacgg gaacgcgacg
     1681 gcgaacaggt tgcccgccgc ctccatgttc ggcacgagca gcccgagcca cacgccgatc
     1741 cagatgaggg cgaaccgcag caggagcagc agcgcgaacg cgtacagcgt ctcaagcggc
     1801 gagccgtcag aacgccagcc tatggccagc gcgatcacgg ccatcaccgc caggtcgagc
     1861 gccgcgtgca gcacgtcgga gacgccgcgg cccgagacga cggccgaggg cgccatcggc
     1921 atcgaacgga accggtcggt gaccccgcgc tccttctcga cgaccacggc catcgcggtg
     1981 ttcatgaagc cgaacgccat cgtcatggcg aacatgcccg gcatcgcgta gtccttgtag
     2041 tccatgccgt cgccgacgtc catggcgctg ccgaagacgt acacgaacag caggaccgag
     2101 acgatcggga agccgagctg ccaggcgatc agcgaaggct gcttcaccag gtggacgagc
     2161 tcgcgcttga cgatggtcca gcagtcgacg aacgcccagt acacccgccg ctccgggccg
     2221 tcctgcccga tgagggcgcc ggccgtcgcg ccggtcgtcg ctcgggtgtc cgcactcacc
     2281 tggtgccccc ctctttcccg tgtccttcgt gtccctcgtg tccctcgcgg tcccggtcgg
     2341 gctcccgcgc gccgctctgc ccggtcagcc gcaggaacac ctcgtcgagc gtgggcctgc
     2401 gcagcccgat gtcctcgcag accaccccgt tctccctgag ggtcccggcc acgtcggtca
     2461 gggcggcgac accgtcgctg accggcgcgt gcacgcgcag gccctcatgg tccgtctcgg
     2521 gccgccggcc cccggcgaca cgcgcgacgg cctcggccgc cgccggcagg tcggccgcgt
     2581 cggacaccac cacctcgacg cggtcaccgc ccacggcccg cttgagcccg gccggggtgt
     2641 cctcggtgat cttgcggccc tggtcgatga gcacgatccg gtccgacagc ttgtccgcct
     2701 cgtccaggta gtgcgtggtg agcagcacgg tcgtgcccag cgccgtcagc ccgcgcacgg
     2761 agtcccagac ctcgctgcgc ccgcgcgggt cgagaccggt cgtcggctcg tcgaggaaca
     2821 gcacctcggg agccaggatc atgccggccg ccaggtcaag ccgccgccgc atgccgccgc
     2881 tgtacgtctt gacctgcttg tccgccgcgt cgagcaggct gaactcggcc agcagctcct
     2941 ccgtcctgcg ccgcgccgtc gcgtggtcga ggtggaagag ccgcccgaac atccgcaggt
     3001 tctcccgggc ggtgagtatc tcgtcgaccg ccgggttctg cccggtcatg ccgatgcgcc
     3061 ggcgcacctt gcgcggctcg cgccgcacgt cgtacccgcc caccagcgcg cgaccgctgt
     3121 ccgcgcgcag cagcgtggac aggatgcgga ccgaggtggt cttgcccgcg ccgttgggcc
     3181 cgagcagtcc gtacaccgcg ccgcgcggca cggcgaggtc gagcccgtcg agcgcggtct
     3241 tgtcgccgta ccgcttgcgc aggccctcgg cgatgaccga gtgatccgat gcggacgcca
     3301 tacgtccccc ctccataact gggtacagtg tactcacaat ggcgtacagc gtaccaagtt
     3361 tttcgcccgg cgcttaggat gggcgcatca tgacgacgca cagcagcggc agcggcgaca
     3421 tcgaccgcag cctcgaactg atgtggcgtc ccgagggacg cccggccagg ggccccaagc
     3481 cgggcctcac cctgtccgcc atcgtcggcg ccgccgtggc gctggccgac agggaggggc
     3541 tcgcggcact ctcgatgcgc agggtggcgg ccgagctggg cgtcggcacc atgaccctgt
     3601 accgctacat ccccggcaag agcgagctgc tcgacctcat gctggaccac gtcgtggccc
     3661 cggtcacgaa gccggagggc ggcgactggc gcgccgcgct cgaacgcatc gccctcgaaa
     3721 ccttcgaact gcacctcgcc cacccctggc tcctccaggt caaccaggcg cgcccgctgc
     3781 tcggccccaa cggcctgctc ggcttcgagt acttcctcgc cgcgctcgac ggcctcgggc
     3841 tcaccggccg ggagcgcgtc aacgtgatca tgaccgtgga cgcgtacgtc tccggcctgt
     3901 cccggcacta cgtcatgctg cgccaggcgc gggccgagtc ccagctgacg gacgaggagt
     3961 tctggggagc gcaggggccg ctcatagcgg acgcgctgaa cagcgggcgg tacccgcacg
     4021 tcatgtcact ccccgaagac gtcttcgagg gcacacccga ggagatactg cgcttcggca
     4081 tcgaccgcgt gctcgacggc gtcgcggcct tcctcgccgc gctccccgcc gaccgccccg
     4141 aggagcggcc gggctggtgc gaggagcccg cccgggacag cggctccgcc tgagccccca
     4201 ccgccggagc gcgggtcgcg cgaccccgca agagcacata tgagtcttcg cccggccacg
     4261 ggaacaccca ccacggacga cgcgatgcac ttctcacttt tttccgcgag ggtcgttcga
     4321 ccgacaatac cgggcggccc cccacggaaa tcggctcgcc ttgtcggtca ttcgaccgac
     4381 aaggcgacga cggggaccgg cagacgattg acgaaatttc cgattgctca attccgccgc
     4441 cacccaggcc acacgcgaag caccgtggct tgatatctgc gcacgggtcg gcagttcgtg
     4501 gcggcgtcca ccgaaaatgg cgaacgacac ggaatgaata aggcgcacga cctcgacaca
     4561 ccacgaatga cctgcggatc cggcatggcc accgtgtgag cgcaggacgg ggaacgccgc
     4621 gtccgcggcc ggaaaaaccc ggcaggatac ccaccggacg ggaatagcaa acgacgctcc
     4681 gccgccctca actggacggc acgaacgccc acttggcctc tcgcacactc attgacatcc
     4741 gcgtaatcgc gtcgctacct tcaagtgaaa gcaattccct ccgcccgaga caatcgagtt
     4801 tttcgggcgc cgaacgggag gcatcatcat gaatgaagaa ctgacccccg acgccaaggg
     4861 cacgacggac agcgcgtccg gtgccgccga caacgcggag acccacacgg ccggccgcgt
     4921 gccgttcacc gtggaaatga tggagctgga caacaacccc atcatcctcc aggcgaagtc
     4981 ctactgaggt ccccgctccc atggccggcc cgcacctggt gaccccactc gccgcacagg
     5041 cgactgggcc gctcggtacc acgggcttcc ggcaccgtct cgccccttgg gtggtcacgg
     5101 ccacccgagg ggcgggcacg ttcgtgctgg gcccgcggct ctccctcgcc cggatcgcgc
     5161 ccggggccca cgaccgggac gaggtcccgg tggcacggtc gatcgcccgg tcgatggggc
     5221 tcgaccgggc cgtggcctcc gggtggctcg aacccgtggc ccccaccgcc ccgcccccgc
     5281 ccgagccgcc ggatgaaccg ccggacgagc cggtgacggg catcctcgtc cattcggacc
     5341 ccgcgacggc ccacgcctgc accgccctcg ccgaacggct gaccgcgcag ggcgcgggcc
     5401 ggtgggtcgc cctcgccgcc gggcagccac cgcccgcgac ggaacgccct cccttcgtcg
     5461 tggtggccgc ccaccgccgc gacgccgcac gggacgccgt gccgccggtg cgggatgggc
     5521 gcgtgctgca cgtcggcgaa gcgttcgaag gcacctacat caccggcccc gggcgcggca
     5581 cggctcccgg ctccgacacc ggggacggct tcgccgcgcg ctgggcgttc gagacccgcc
     5641 tcgcgtcgct cggcctcacc gaccgccccg tgccgctcct gttccgcatc cgcgaggacc
     5701 cggccgccgt ggtccgcgcg atcatgaccg ccatggccct gccgccgagc ggcgccgtcc
     5761 tcgtcgcgac cgggcgcacc gtgcgcttct gcacgccgga cgcggtcggt tcgacccccc
     5821 tgcgcgacgt gccgcgcacg caggcgtgga cgttcggaat ggcccgcggg ttcggcgtcg
     5881 ggcccggctc ggtccccggc agctacatcg ccacctgccg cacccccgcc ggcggccagg
     5941 accacctgga gggcaacagc ggcaagggcc cgacggaaga ggccgcgacc acgggcgccg
     6001 tcggcgaagc gctggagcgg tacgcggcct acgaggccaa ctggagcctg ccgccgagtc
     6061 gcggccccgc gcgccggatc gacctcgcgg acctgcaccc gtacggcgac gcgtgggagc
     6121 ggcgcgtcgc ggccggcgcc gcggccggcc cgcccgtggc cttcgtcgac ggcgccggcc
     6181 tcgccgacgg cgccgccgtc gcggtaccca gggccctcgt cgccttcccc tacgtggggg
     6241 cggaccgccc gacggacggg accaccaccg gcctggcggc cggccccgac ctcgccgccg
     6301 cgtcgctgcg cggcctgcgc gaggtcctgg aacggcacag cctgtacggg tcgttcgcgc
     6361 acctgcgtcc ggggtaccgg ctcgacccgg ccgcctcgct cgaccgcctg ggcctgaccg
     6421 gcgcgttcaa gggagaggtg tgggccgtcc actggccgca cgagggctac gtcctgccgg
     6481 acgtccacgc gttccaccac gacccagaag agggcctgct ggtgcgggcg agcggttcgg
     6541 gactcacctt cgacgaggcg atggacagcg ccctcgcgga gctgtgccag gtgcacttcg
     6601 agggagtgcg ggcgcgccgc gcgggtgcgc cggccggccc ggcgcacgcg gcgtgggcgc
     6661 gaaggcccgt cgtgggccgg gcccgccgct acctggacgc cctgcccgcc gccgccgcgc
     6721 caccggtgcc gtacacggac ccgcgcgcgc agttcgacca cctcgtgagc cgcctcgccg
     6781 accgcggcac cggcccggtc gtggtccggc tccccctgcg cgggccggac tggaccgtgg
     6841 tccgcgtgct ggtcccgggg gcgacgacgg cgacagctcc ctccgacagc cgcggcggac
     6901 gtcgcctggc cgacgcccca tggacccatg gcataccgac ctgagccgcc gccttccgca
     6961 ccgcacccga acgccggaca catcacgcag aaagccgaac gccgaacgcc gacagaccga
     7021 ctcgccgcat ccgaacgccg aaaggaagcc gctgtgaaga ccctggacgc cttccgcgat
     7081 ctcgaagagg cgctggtcgc cgccgacacc gccgccggtg ccccagcacc cggctccgac
     7141 gcccgccggg cgcacgccct ggccgaagtg ctcgcggacg cgcgggacat cgcgcgggcc
     7201 gaccccggcc tgctgctggc cgacctggtg tcggacccgc gcggctggcc gccccagtcg
     7261 tggaagaact acttcggcaa cgcctgcgtc accgtcatcc gcaccgcgac cctgcgggtc
     7321 gacgtcctgt actggctcca gaacgcctcg acgctgcaca agcacgtgtc gtcaggggcg
     7381 ttcctcgcgc tcagcggccg gcggctgcat ctcgaatacg acttctcggc cctggactca
     7441 ctgaacgccg gcgtgacgtt cgggcagctc tcggccaccg gcaggagcat gatgcgcgag
     7501 gccgccgtct cgccgatcct gccgacgatg gtgcacgagc tgtactggat cgagaagccg
     7561 gcggtgaccg tgtcggtccg ctgcgcgccg gcccccggct ccggccggag cgacccggac
     7621 gcgccgcggc cggcgccgca ccgcccgcac gagttcatcg cgccgggcgc cgggtacctg
     7681 cccgccgcct tccacgacac ctcgaacgtg cagcggtggc tcgacggact cggcatgctg
     7741 cggcgcgcga accgcgagct gtacctcggc gcgctcgaca cggccctgcg cctggtggac
     7801 tcgatccacc tcatccacgt cctcgacgaa ctgtgcgaca accccgtcga cgaggtctcc
     7861 gccctgctgg cacgcgccga cgcggcgcgg gaggacgacg aactggcgcg cctggcccct
     7921 tcggtgcccg agttccgccg ccgcaagatc ttctcgcgcg cctacgccaa gggccctgac
     7981 acccagctgc tcgcggcgct gctgtgggcc ggcgccgagg gcggcgaact ggccgccctg
     8041 ctcaaggccg agaaggtggc ggacccggag gagttcgtgc gcgtccacgg cgaacggctg
     8101 agcgccgtcg accccaggat cgcgccgtac gccgagagcg cgcggcggtc actgcgatga
     8161 acgacggctg ggacatcgcg tcgttctggg agaaggagcc gcgcgtcctc gacggcccgc
     8221 gcctcgacga ggaaacgctg ttcggcctcg ccgtcgcggc ggcggcccgg cgcgccgggc
     8281 tgcccgaccc gcaggatgtc cgcgtgttcg cggacgacgg gaccgtcacc ccggcccggg
     8341 tggacgccta cctgccgtcg gcaggacgcg acggcagctt cgcgcgctac gccgagcgga
     8401 tgctcgccgc ggtcggctcc ttcgccctga ccatcaattc gctccagcag ttcggctggg
     8461 acttctggca gaccctgcgc cgcgcgaccg atcccgtgct ggccgccggg ggctggtcgg
     8521 cgggcggcgt cgactgccac ctcatcgcgt cggtctacgg caccgccccg acgctggtcc
     8581 acaaggacac cgcgggcgtc ttcacgtacg tcgtgcgcgg cttcaaacgc ttctacacct
     8641 ggccctacga ggtattcgcc ggcatcgcgg gcgaggaggc gacgcagcgg caggtgaacc
     8701 tgcccgcgtc catccgcccc gaggaccacc tggacaccgc gacggtcgtc gagggcggac
     8761 ccggctccgt tctgtactgg ccttcggacc ggtggcactg cgcgatgtcg gacggcaggc
     8821 tggccgtctc gctgcacctc gcgcactacc agtgggacga ccgcctcgcg ctgctgctgc
     8881 gccgcgtgcg acggctggcc gcgtcggagc tcggcaccgg caggttcgag ggcgggccgg
     8941 tgcccgaggc cggccccggc ggcgaccgct ccaccgacgc gcaggtccgc aaggtcgtgt
     9001 cccggctcgt cgacgactcg gcactcgacc tcgacctgcg gctccgccgc ctcaagcggc
     9061 gcaccgcctc gaacttcgag gtgatacccc cgccgcggcc gtgtcccgac ctgtcggcga
     9121 cgcccggccc gctgaccgtg ccggaggggt cggtggcgcg gacactgacc gccgacgggg
     9181 tcacctacct cgcggtcaac ggccacatcc tgcggatcac gggcggcgcg tggctcgacg
     9241 cgttcctgcc ctggctcgtc cccggcgccg cgcacagccc cgaggagtgg gcgaacgccg
     9301 ccgccgcggc cggcggcccg tcgggtcccg agtgcacggc gttcctgggc gaactggtca
     9361 agcggggggc gctcaccccc ggggaggcgg acgcgtgagg ctcaacgacg gcgcgttcgt
     9421 cgcggtcaag gacggccagc tgatgctggg gcacgtcgac cgcccgttcg acctctggcg
     9481 ggccgacgcc ggcgtgctcg tcctcctcgc ggagatcgcg cgcggcacac cggccgggga
     9541 actgccggac gcgatggccg ccgtggtggg cgcggccggc gagtcggccg tgcccgccgc
     9601 cctggcgcac ctgcgccgcg cgcacgtcgt cgccgacggc gccggcgcgg cgctgcgcga
     9661 cgtgaacgcc gaggtcgcga aggtcatcgg cgcggcgccc gacctgcacg cggacaccgg
     9721 tttcatgcgg gcggcccgcg cgtgcgaggg gctgaccctc acgtcggccg ccgcccagta
     9781 cgccctgtgg tcggcggtgc ggcacgtgat cgccgccggg gtgcccggag ccctggtcga
     9841 gtgcggggtg tggcgcggcg gcagcatgct gctcgccgcg ctcgccctgc tgcgggacgg
     9901 cgtgcgcgat cgcgacctgt tcctcttcga caccttcggc tggagctggg agcggacggg
     9961 cgagcacgac ggcttcatgg agccgggggg aacggcgcgg gccccggagc gcacgcccgc
    10021 cgaggaggcg cccgccggat ctgccgcgcc ggggccgatg gacgtgggcg tgtcggccga
    10081 cgaggtgcac gcgcggatca ccgccgccgg ctacccggcg gaccgcgtgc actgcgtcgc
    10141 cggcctcgtc caggacacgc ttcccgcgcg ggctcccgac cgcatcgccc tgctgcggct
    10201 ggacaccgac cagtacgagt cgaccctgca cgaactgcgc gaactctacc cgcgcgtggc
    10261 ccctggcggg ctcgtcatca tcgacgacta cggcaagctc gccggcgcca ccagggccgc
    10321 ggacgcgtac ctggccgggc tcgaccgccc ggccctgctc caccgcatcg acacgcaagg
    10381 ccgcatcttc gtcaagcccg atgagaggta ggacccatgg ttgctgtcat cgatcacgcc
    10441 gtgcgcccgc ggttcagccg tcatgtctcc ttcgccctgg acgaccacga ggtcaggatc
    10501 gaggacggca cgtaccgggc cgcgttcccg gccggcggcg tcacgctgcc cgacctggac
    10561 cgcctcgcgg ccggcgtgtc cccggacgag ctggtcgccg gcgtccgcgg ctggctcggg
    10621 tgcggcgagc cggacgcgat cgccgtcatc gagcgcctgt tcagcaccgg cctgctcgtc
    10681 gacccggaag cggcgcgcct cgactccgtt cccggctcct acgtcgcctg ccggctcgtc
    10741 gacgcgttcc ggcgggagtt cccccgcgtc gtctccggct cgccgctgct gcgcgacctg
    10801 gccgccgggc gcgaccgcgg cctggcgctc ggcttcctgc tggagaccta cttcgtggtc
    10861 cgggccgcgg tctggacgac cgaccccgtt ttccggcacc ccatgacccc ggcccagcgc
    10921 gaggccctgg acgacttccg ggcgtcggag tcggggcacg gcgaactgct cctcggcggc
    10981 ttctcggccg ccgggttcga caccgacgcg ctgcgccgca gccaggccgc cgtcgagacc
    11041 ttggcctacg cgcagtccta cggcgcgttc gcgtggcagg gggtcgcgga gttcgccgcg
    11101 tccctggtgc tgcccgaggt gccggcgctc ggcgcggacc cggccgcccc cggtgtcgac
    11161 gtgctcgacc tgatcgaaca gcgacacggg gtgcccggca gcctgatcgc ccggttccgg
    11221 gcccacgacg ccgaggacgt cgaggccgac cacggcgggc tgcccggcat cctgctgtcg
    11281 gaggaacggg acctgacgcc cgcgaaggtg gaacggctgt tcgtcgtcct gcggcacatg
    11341 atggacctct accgcagcca cctcgacgcg gtgcaccggc gctacgcgca ctgggacccg
    11401 gacgcggcag ccggcccgga actgcccgac aacgccctcc ggttctgatc cggcgagcac
    11461 gccgaggaag gcgttcacca cacgacatga cagccaccgg tttttacgac ggtctcatcg
    11521 ggaccgtgta cgcttccgcg atcgccgacc aacacttcgg tgaggacgcg gtgttcgccc
    11581 gggtcgtcgc ccgcgccggc ggtaccgcgc tcgaactggg cagcggcacc gggcgcctgc
    11641 tgctgcgcct gctcggcgcg gggcactccg tgcacgggct ggagatctcc gaggagatga
    11701 ccgcccggtg ccgggccgcc gcggcacgcc aggggctcag ccccgtggtg caccgcggca
    11761 gcttcgcgcc cctggacggg gcgctgagcg ggtacgcggc gatgtactgc ccgctcaacg
    11821 cgttcagctt cgtcatcgac gacgaactcg cccgcgcgtc actgcgcggc tacgccgccg
    11881 cgctcgaaac gggcggcacg ctcgcgatca ccggatcggc cggcgacccg gccgcgctgc
    11941 gcgc
//
