LOCUS       NZ_CP084541            10396 bp    DNA     linear   CON 20-NOV-2024
DEFINITION  Streptomyces marincola strain SCSIO 64649 chromosome, complete
            genome.
ACCESSION   NZ_CP084541
VERSION     NZ_CP084541.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces marincola
  ORGANISM  Streptomyces marincola
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6629020)
  AUTHORS   Shi,S.
  TITLE     Strepomyces indimicinicus sp. nov., an antibiotic producing
            actinomycetes isolated from the marine environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6629020)
  AUTHORS   Shi,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2021) Key Laboratory of Tropical Marine
            Bio-resources and Ecology, South China Sea Institute of Oceanology,
            Chinese Academy of Sciences, 164 Xingang West Road, guangzhou,
            Guangdong 510301, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. V6.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 444.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020410765.1-RS_2024_11_18
            Annotation Date                   :: 11/18/2024 03:45:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,763
            CDSs (total)                      :: 5,689
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 74
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 21 of 77
            Pseudo Genes (incomplete)         :: 60 of 77
            Pseudo Genes (internal stop)      :: 2 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:57:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 961161
            Orig. end                         :: 971557
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10396
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="8"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10396
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10396
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5396](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5396
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(355..1254)
                     /locus_tag="LC193_RS04120"
                     /old_locus_tag="LC193_04120"
     CDS             complement(355..1254)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008748648.1"
                     /locus_tag="LC193_RS04120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_04120"
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_226071645.1"
                     /transl_table=11
                     /translation="MNRTARRGATALLAATVLTTPATARADEAGDRSAGTASAAVLRAG
                     LNVSLLDGGVRLPLTASLNEVSAPDGGGSADDTLLTATLEGVEDGQPFDLLRADAASAS
                     ATTGADGAFAETSLAGARVHLPGLPLLSLIEVDAVSARASCPVGGQASAETVMPATATV
                     LGRTAELSTEGTTEVSVPGVGDVTLALAQRESGDGTASASALDLNVAVDPLDLGVAEVT
                     GTVTLAEAACTAPAPPGRGPQTVTEDQPVERPDEDVDLAETGGGSSTPYMVGGAAGLLA
                     AGAATVLIARRRRAAAQG"
     gene            complement(1361..2485)
                     /locus_tag="LC193_RS04125"
                     /old_locus_tag="LC193_04125"
     CDS             complement(1361..2485)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008748647.1"
                     /locus_tag="LC193_RS04125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_04125"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_226071646.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.8e-18, bitscore:
                     65.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAGPPSAPVLHIRGRVLAAEGPDGGPDLVIRDELWVVGGRITYD
                     RPPAARDVVRLTGWVLPGLVDAHCHVGLDAHGAVGRAAAEAQALADRDAGALLLRDAGS
                     PSDTRWTDARADLPPIIRAGRHIARTRRYIREYAHEIEPDRLAAVVRQEARRGDGWVKL
                     VGDWIDRERGDLAPCWPADALVAAVAAAHEEGARVTAHCFAEDSLADLVAAGIDCVEHA
                     TGLTEDTIPLFAAHGVAIVPTLVNIATFPGLAESGEAKYPRWSAHMRRLHARRYDTVRA
                     AHDAGIAVFTGTDAGGSLPHGLIAREVAELCRAGLPVHDALAAATWRAREWLGRPGLTE
                     GAPADLVVYADDPRRDLAVLAEPRRVVLRGRVVA"
     misc_feature    2727..2740
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.89"
                     /tool="antismash"
     misc_feature    2870..2887
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.31"
                     /tool="antismash"
     misc_feature    complement(2872..2887)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2882..2897
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            3054..3635
                     /gene="ectA"
                     /locus_tag="LC193_RS04130"
                     /old_locus_tag="LC193_04130"
     CDS             3054..3635
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577188.1"
                     /locus_tag="LC193_RS04130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_04130"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_404819345.1"
                     /transl_table=11
                     /translation="MTPAQADHARARPDFMDSEFTAGTDGLTLDTPRLDDGADLWRLAR
                     DSRVLDLNSSYSYLLWCRDYARTSIVARDAAGVPVGFITGYTRPQAPSTLLVWQVAVDG
                     GQRGRGLAAAMLDALARRTGRELGVNMIETTVTPDNTASNRLFASFAARHDAAVEREVL
                     FDAGVFPERGHQPEVLYRIGPIAEPAATAR"
     gene            3702..4964
                     /gene="ectB"
                     /locus_tag="LC193_RS04135"
                     /old_locus_tag="LC193_04135"
     CDS             3702..4964
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-363). E-value: 5e-73.
                     Score: 238.0. Matches aSDomain:
                     nrpspksdomains_LC193_RS04135_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141823.1"
                     /locus_tag="LC193_RS04135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_04135"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_226071650.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.8e-75, bitscore:
                     251.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFTTLESEVRSYCRAWPTIFDRAQGSYLYDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALLDYLERDGITHGLDMSTTAKRAFLESFQNVILRPRD
                     LDYKVMFPGPTGTNAVESALKLARKVKGRESIISFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDDYLDGRTPDFIWFERLLEDTGSGLNKPAATIVETVQGEGGINVARP
                     EWLRGLADLCRRHDMLLIVDDIQMGCGRTGPFFSFEEAGIVPDIVTVSKSISGYGLPMA
                     LTLFKPELDIWGPGEHNGTFRGNNPAFVTAAAALETYWNDGQMEKQTLGRGEQVESALR
                     AIAAEHSGASYRGRGMVWGLTFEDTERATAVCRRAFELGLLLETSGPQSEVVKLLPALT
                     TTPEELDEGLRVLARAVRETD"
     aSDomain        3789..4790
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LC193_RS04135_Aminotran_3.1"
                     /evalue="5.00E-73"
                     /label="LC193_RS04135_Aminotran_3.1"
                     /locus_tag="LC193_RS04135"
                     /protein_end="363"
                     /protein_start="29"
                     /score="238.0"
                     /tool="antismash"
                     /translation="DRAQGSYLYDEDGHTYLDFFAGAGSLNYGHNNPVLKRALLDYLER
                     DGITHGLDMSTTAKRAFLESFQNVILRPRDLDYKVMFPGPTGTNAVESALKLARKVKGR
                     ESIISFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDDYLDGRTPDFIWFERL
                     LEDTGSGLNKPAATIVETVQGEGGINVARPEWLRGLADLCRRHDMLLIVDDIQMGCGRT
                     GPFFSFEEAGIVPDIVTVSKSISGYGLPMALTLFKPELDIWGPGEHNGTFRGNNPAFVT
                     AAAALETYWNDGQMEKQTLGRGEQVESALRAIAAEHSGASYRGRGMVWGLTFE"
     gene            5001..5396
                     /locus_tag="LC193_RS04140"
                     /old_locus_tag="LC193_04140"
     CDS             5001..5396
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384690.1"
                     /locus_tag="LC193_RS04140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_04140"
                     /product="ectoine synthase"
                     /protein_id="WP_086161249.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.6e-53, bitscore:
                     178.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLSDIEGTDRDVQSASGTWRSKRIVLAKEGVGFSLHETVLY
                     AGTETTMWYANHIEAVFCVEGEAELTNEETGEKHWISPGTMYLLDGHEKHTIRPKSDFR
                     VMCVFNPPVTGREDHDENGVYPLLTEA"
     gene            5417..6316
                     /gene="thpD"
                     /locus_tag="LC193_RS04145"
                     /old_locus_tag="LC193_04145"
     CDS             5417..6316
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358305.1"
                     /locus_tag="LC193_RS04145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_04145"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_226071651.1"
                     /sec_met_domain="PhyH (E-value: 6.7e-37, bitscore: 126.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGATEVTTPRQDPVVWSEPGAPGPFSTDELA
                     GFERDGFAAVDQLITPEEVLAYRAELDRLVTDPDIRADERSIVEPSSQEIRSVFEVHKI
                     SEKFAGLVRDPRLLDRARQILGSEVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     RMRTVSVSIALTENFDTNGGLMIMPGSHLSFLGCAGETPKDNYKKSLQMQDAGTPSDEA
                     LTGFADRHGIKLFTGRAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENAAVEPFA
                     APVRRPEFIGARDFTPVS"
     gene            6478..6876
                     /locus_tag="LC193_RS04150"
                     /old_locus_tag="LC193_04150"
     CDS             6478..6876
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC193_RS04150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LC193_04150"
                     /product="ATP-binding protein"
                     /protein_id="WP_226071654.1"
                     /transl_table=11
                     /translation="MDTDSDAWSRWVTLPDGSPDRIWREREITALLTGHTGGHLFLAGC
                     KSNQGGFYPLLDQVVLLSAPADVLLARIERRTGNPYGKRPEERAAVLRHLATVEPLLRA
                     TASAEIDATAPVADVVGQVESLAGPLPA"
     gene            complement(7061..8071)
                     /locus_tag="LC193_RS04155"
                     /old_locus_tag="LC193_04155"
     CDS             complement(7061..8071)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019365.5"
                     /locus_tag="LC193_RS04155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_04155"
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="WP_318842125.1"
                     /transl_table=11
                     /translation="MRGVDLSSVPKAEDLGAVFRYADGTVGDPVRILRDGGANWVRLKV
                     WVDSPDGYHGTAQVLTMARRAHALGMRLLVDFHYSDSWADPGQQRKPAAWTGLRGSALE
                     QALYDHTSDVLGALRAQGTPAAMAQTGNEINHGLLWPEGSTDDWNALAGLLRAGSRAVA
                     DASPGTEVALHLAEGGDNGGTVWWFDQAVARGVPFDVIALSFYVYWHGTLDDLGANLAD
                     AAARYGKDVVLAETAYPFTLRDADGHPNLIGDTGQLPPAFPPTPQGQAAWLDAVSGVVG
                     AVPGGRGRGLFYWEPAWIATRGNGWDPADPASGNAWENQALFDFTGRALPAARWR"
     gene            complement(8215..10215)
                     /locus_tag="LC193_RS04160"
                     /old_locus_tag="LC193_04160"
     CDS             complement(8215..10215)
                     /GO_component="GO:0009341 - beta-galactosidase complex
                     [Evidence IEA]"
                     /GO_function="GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174265.1"
                     /locus_tag="LC193_RS04160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LC193_04160"
                     /product="beta-galactosidase"
                     /protein_id="WP_226071656.1"
                     /transl_table=11
                     /translation="MDRLAFGGDYNPEQWPEELWPEDVALMREAGVTLVSIGIFSWARL
                     EPREGVYDFGWLDRIIDLLHEGGIRIDLATPTVVPPAWFYRAHPEALPVTREGVRLEFG
                     SRGAICHSSPAYREAAARITRALGERYGDHPALALWHVHNEYGVPVLECYCDVSAAHFR
                     RWLAERYGTLDALNAAWGTAFWGQAYGEWDEVQPPRLTPTTANPAQRLDFARFASDSAL
                     ENFRAERDLLHELSPGVPVTTNFMVSPTQCDTIDYWKWGREVDIVTNDHYLVAEDDRNH
                     INLSFAADLTRSVGAGRPWLLLEHSTSAVNWQPRGIAKRPGEMARNSLAHVARGSQGAM
                     FFQWRASRAGAEKFHSAMVPHAGTDSRVWREVVELGGHIDALADIMDTRTVADAAVVWD
                     WESWWAHKLEWRPSDDLGTRERAEAWYAALFDQHLTVDFAHPESGDLAGRPLVVVPALY
                     LATRAAGENLRRYVEGGGTLVVSCFSGIVDEHDAVHPGPHPGALRDVLGLVVEEFQPLR
                     AGETVRLAGPEGATATADVWAETVRTTGAETVWTYADGPAAGGPAITRHRLGDGTAWYV
                     SARVGQEGLRQVLAAAGKDAGLDPRDDLPRDVEVVRRAGPGGDFLFAINHTAEDHEVAL
                     PASGTELLTGTRADGPLAVPAGATRVVRLDR"
     misc_feature    complement(10177..10196)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.54"
                     /tool="antismash"
ORIGIN
        1 ggacccccga gggggtgacg agcacggcgt cccccacgcg gacggacacg ttgcccgagg
       61 tgccgacgac gaggccgtcc gccaccgtcc gccgcgcgtg cgcgaccagg tcgtgccacg
      121 cctcctgcca cgcgtccggc cacccgccgg acgtctcgcc ctgcccttgt ccgctcatcg
      181 ccgcgtccac ccgtctcccc tgtaccgctc gtcatcggcc gcaccgcccg tcgtcggcgc
      241 cgccggccac gggtgattgt ccggcaggcg gggcacgcgg cgacagggga acgggccggg
      301 cgcgcgcgag gcgcggcgcc cggcccgcgt tcccgcggcg ccgcgccggg ccggtcagcc
      361 ctgcgcggcg gccctgcggc ggcgggcgat cagcacggtg gccgcgccgg cggcgagcag
      421 gccggccgct ccgccgacca tgtagggcgt ggacgagccg ccgcccgtct ccgccaggtc
      481 gacgtcctca tcgggccgct cgaccggctg gtcctccgtg acggtctgcg ggccccgccc
      541 gggcggggcg ggcgcggtgc aggcggcctc ggcgagcgtg acggtgccgg tgacctcggc
      601 gacgcccagg tcgagcgggt cgaccgccac gttcaggtcg agcgcgctcg cgctcgcggt
      661 gccgtcgccc gactcgcgct gggcgagcgc gagggtgacg tcgccgacgc ccggcaccga
      721 cacctcggtg gtgccctcgg tgctcagctc ggcggtgcgg ccgagcacgg tggccgtggc
      781 gggcatcacg gtctcggccg acgcctgccc gcccaccggg cacgaggcgc gcgcggagac
      841 cgcgtccact tcgatcaggg agagcagcgg caggcccggg aggtggacgc gggctccggc
      901 gaggctcgtc tcggcgaacg cgccgtccgc gccggtcgtc gcggaggcgg aggcggcatc
      961 ggcgcgcagc aggtcgaacg gctgcccgtc ctcgacgccc tccagggtcg cggtcagcag
     1021 ggtgtcgtcg gcgctgccgc ccccgtcggg cgcgctgacc tcgttgagcg acgccgtgag
     1081 cgggagcctg acgccgccgt cgaggaggga gacgttcagg ccggctcgca ggactgcggc
     1141 gctcgccgtc cccgcgctcc ggtccccggc ctcgtcggca cgggccgtgg ccggggtcgt
     1201 caggacggtg gcggcgagca gcgcggtggc gccgcggcgg gcggttctgt tcacgacagg
     1261 ggactccatc gggatgggtg ttgctgggac gcccgccatc cttcggtccg ctcgcgccgg
     1321 ccgtcctggt tacgtcaccc gttcacccga acggggtaac tcaggcgacg acgcgccccc
     1381 gcagcacgac gcgtcgcggc tcggcgagta cggcgaggtc gcgccgcggg tcgtcggcgt
     1441 agacgacgag gtcggcgggc gcgccctcgg tcaggcccgg gcggccgagc cattccctgg
     1501 cccgccaggt cgccgcggcc agcgcgtcgt ggacgggcag tccggcccgg cacagctccg
     1561 cgacctcccg cgcgatgagg ccgtgcggga gcgagccccc ggcgtccgtt ccggtgaaga
     1621 cggcgatgcc cgcgtcgtgg gcggcgcgca cggtgtcgta cctgcgcgcg tgcagccggc
     1681 gcatgtgggc cgaccagcgc gggtacttgg cctccccgct ctcggcgagg ccggggaagg
     1741 tcgcgatgtt gaccagggtg ggaacgatgg ccacgccgtg cgcggcgaac agggggatgg
     1801 tgtcctcggt gagtccggtc gcgtgctcca cgcagtcgat gcccgcggcc acgaggtccg
     1861 cgagcgagtc ctcggcgaag cagtgcgcgg tgacccgcgc gccctcctcg tgcgcggcgg
     1921 cgacggcggc cacgagcgcg tcggccggcc agcagggcgc gaggtcgccg cgttcgcggt
     1981 cgatccagtc cccgacgagc ttgacccagc cgtcgccgcg cctggcctcc tgcctgacca
     2041 cagcggcgag ccggtcgggc tcgatctcgt gcgcgtactc gcggatgtag cgccgggtgc
     2101 gggcgatgtg ccggccggcg cggatgatcg ggggcaggtc ggcgcgcgcg tccgtccagc
     2161 gggtgtcgga gggcgacccg gcgtcgcgca gcagcagggc gcccgcgtcg cggtccgcga
     2221 gggcctgcgc ctccgcggcg gcgcggccga cggccccgtg cgcgtcgagg cccacgtggc
     2281 agtgggcgtc gacgagcccg ggcagcaccc agccggtgag gcggacgacg tcccgcgcgg
     2341 cgggcggccg gtcgtaggtg atccggccgc cgacgaccca gagttcgtcc cggatcacca
     2401 ggtcgggtcc gccgtccggt ccctcggccg cgagcacgcg gccccggatg tggaggacgg
     2461 gcgcggaggg cgggcctgcg ttcatgcgcc gcagcctacg gccgggccgc cgcccgcgcc
     2521 gagggccggt gcccgaactc gtgggcgggg cggccccggt ggacgactcc gatggacgac
     2581 tccgatggac gacttcgatg gagcggccgc cgcggggccc gcgcgccgcc ccgcgcccgg
     2641 gaacgggcgc gaggggttcg gggcaccggg tccgcgggtg gcggtccccc ggctgccgcg
     2701 cgataattcc cggcgcagct cccggcgttt tacaacggaa ttcaaactgc gattccccag
     2761 gtaattcaga cggagccaac gggcctcttc tgcccgcttt atcgacttct aaacggggcc
     2821 gatacacgat cgtggccggt caccggaacg ggtcagcacc ctcacttctt cgttacagac
     2881 atgtgaccaa gcccacacgg atgaccgatt tgcccggtaa ctaacggaca gaacgtaact
     2941 ttttgctccg aagtaagctg cggtcccccg cgcgcgcgat aacacatcgg gcccgacctg
     3001 ttaagaccag tcggcggtgc aatttcaaga atcgctgggt aaattgaatc tgcatgaccc
     3061 ccgcacaagc agaccatgca cgtgcccgac cggatttcat ggacagtgaa ttcaccgcag
     3121 gaaccgatgg cctcacactc gacaccccgc gcctggacga cggagcggac ctgtggcgcc
     3181 tcgcccggga ctccagggtc ctggacctga actcgtcgta cagctacctc ctgtggtgcc
     3241 gcgactacgc gcgcacctcg atcgtggcca gggacgcggc cggcgtcccg gtcggcttca
     3301 tcaccgggta cacccgcccg caggcgccga gcaccctgct ggtgtggcag gtcgccgtcg
     3361 acggcgggca gcgcggacgc ggcctcgccg ccgcgatgct tgacgccctg gcccgccgga
     3421 cgggccggga gctgggggtg aacatgatcg aaaccaccgt cacaccggac aacaccgcgt
     3481 ccaaccgcct cttcgcctcc ttcgccgcgc ggcacgacgc cgccgtggag cgcgaggtgc
     3541 tgttcgacgc gggggtgttc ccggaacggg ggcaccagcc ggaggtgctg taccggatcg
     3601 gccccatagc cgaaccggcg gcgaccgccc gctgactccg acccgaccgc gaccacttcc
     3661 gacccctcca cgtgatcacc ccctgagatc aggagaccgt tgtgaccatc acccagcccg
     3721 acctgagcgt gttcaccacc ctggagtccg aggtccgcag ctactgccgc gcctggccca
     3781 ccatcttcga ccgcgcgcag ggcagctacc tgtacgacga ggacggccac acctacctgg
     3841 acttcttcgc cggcgccggc tcgctcaact acggccacaa caacccggtg ctcaaacgcg
     3901 ccctgctcga ctacctggag cgcgacggca tcacccacgg cctggacatg tccaccaccg
     3961 cgaaacgggc gttcctcgaa tcgttccaga acgtgatcct gcgcccgcgc gacctcgact
     4021 acaaggtgat gttccccggc ccgaccggca ccaacgcggt ggagtccgcg ctgaagctgg
     4081 cccgcaaggt caagggccgc gagtcgatca tctccttcac caacgcgttc cacggtatgt
     4141 cgctcggctc gctcgccgtg acgggcaacg cgttcaagcg cgccggcgcc ggcatcccgc
     4201 tggtgcacgg caccccgatg ccgttcgacg actacctcga cggccgcacc ccggacttca
     4261 tctggttcga gcggctgctt gaggacaccg gttcggggct gaacaagccc gcggcgacga
     4321 tcgtggagac ggtgcagggc gagggcggca tcaacgtggc gcggcccgag tggctgcgcg
     4381 gcctggccga cctgtgccgg cggcacgaca tgctgctgat cgtcgacgac atccagatgg
     4441 gctgcgggcg caccgggccg ttcttctcgt tcgaggaggc gggcatcgtg cccgacatcg
     4501 tcacggtgtc gaagtcgatc agcggctacg ggctgccgat ggccctcacg ctgttcaagc
     4561 ccgagctcga catctggggc cccggcgagc acaacggcac gttccgcggc aacaacccgg
     4621 ccttcgtcac cgccgccgcc gcgctggaga cgtactggaa cgacgggcag atggagaagc
     4681 agaccctcgg ccgcggcgag caggtggagt ccgcgctgcg cgcgatcgcc gctgagcact
     4741 ccggcgcctc ctaccgcggc cgcggcatgg tgtggggcct gacgttcgag gacaccgagc
     4801 gcgccaccgc cgtgtgccgc cgcgcgttcg aactcggcct gctgctggag acgtcgggcc
     4861 cgcagagcga ggtcgtcaag ctgctgccgg cgctgacgac cacgcccgag gagctggacg
     4921 agggcctgcg ggtgctggcc cgtgccgtcc gcgagaccga ctgaagcaac cactgggaac
     4981 gggaagggaa gtacaccgcc gtgatcgtcc gatcgctcag cgacatcgag ggaaccgacc
     5041 gggacgtgca gtccgcctcc ggcacgtggc gcagcaagcg catcgtcctg gccaaggagg
     5101 gcgtcggatt ctcgctgcac gagaccgtgc tctacgcggg taccgagacc accatgtggt
     5161 atgcgaacca catcgaggcc gtcttctgtg tcgagggcga ggccgaactg accaatgagg
     5221 agaccggcga gaagcactgg atctcgccgg gaacgatgta tctgctcgac gggcacgaga
     5281 agcacaccat tcgtccgaag agcgacttcc gcgtcatgtg cgtcttcaac ccgccggtca
     5341 ccggccgtga ggaccatgac gagaacggtg tctacccgct gctcaccgag gcgtaaggag
     5401 aggaaggaac acccacatga ccaccgcacc cgaacgcacc gccgacctgt acccgacccg
     5461 cggggcgacc gaggtcacca cgccacgcca ggacccggtg gtgtggtcag aacccggggc
     5521 gccggggccg ttcagcacgg acgagctggc cggcttcgaa cgggacggct tcgccgccgt
     5581 cgaccagctg atcacgccgg aggaggtgct ggcctaccgc gccgaactgg accggctggt
     5641 caccgacccg gacatccgcg cggacgagcg ctcgatcgtc gagccctcgt cgcaggagat
     5701 caggtcggtg ttcgaggtgc acaagatcag cgagaagttc gccgggctgg tgcgcgaccc
     5761 gcggctgctc gaccgggcgc ggcagatcct gggctccgag gtgtatgtgc accagagccg
     5821 gatcaacgtc aagccggggt tcggcgccag cgggttctac tggcactcgg acttcgagac
     5881 gtggcacgcc gaggacgggc tgccccgcat gcggacggtg tccgtgtcga tcgcgctgac
     5941 ggagaacttc gacaccaacg gcggcttgat gatcatgccg ggctcgcacc tgtcgttcct
     6001 cggctgcgcc ggtgagacgc cgaaggacaa ctacaagaag tcgctccaga tgcaggacgc
     6061 gggcacgccg tcggacgagg cgctgaccgg gttcgcggac cggcacggga tcaagctgtt
     6121 caccggccgg gccggctcgg ccacgtggtt cgactgcaac tgcatgcacg gctcgggcga
     6181 caacatcacg ccctacccgc gcagcaacgt gttcatcgtg ttcaacagcg tggagaacgc
     6241 cgcggtcgag cccttcgcgg caccggtccg ccgtcccgag ttcatcgggg ccagggactt
     6301 cacgccggtg agctgagccg gtttcaccgg ccggcgggcg ccggttcccc gcgaggggga
     6361 ccggcgcccg ccgcgttcgc gtaggctctc ggcccgtggc caaggtcctg ctcaccggca
     6421 tgtcgggcac cggcaagtcg acggcgctgc gcctgctgcg cgcgggggca caggacggtg
     6481 gacaccgaca gcgacgcctg gagccgttgg gtgaccctgc cggacggctc ccccgaccgg
     6541 atctggcgcg agcgggagat caccgccctg ctgaccggcc acaccggtgg ccacctcttc
     6601 ctcgccggct gcaagagcaa ccagggcggc ttctatccgc tgctcgacca ggtcgtgctg
     6661 ctcagcgcgc cggccgacgt gctgctcgcc aggatcgagc ggcgcaccgg caacccctac
     6721 ggcaagcggc cggaggaacg ggccgccgtg ctgcgtcatc tggccaccgt cgaaccgctg
     6781 ctgcgagcca ccgcgagcgc cgagatcgac gccaccgcgc ccgtggcgga cgtggtcggg
     6841 caggtggaga gcctggccgg gcccctgccc gcgtgaccct ccgcggccga acggccaggc
     6901 ggcggccggg cgtcagacga acaggtggcg gttctcccgg ctccacacga cgcggctttc
     6961 gagttcgccg acgcgcacca cgcggcggtg cggctcgtcc tgcgacacct gttccacggc
     7021 catgcgggca gtatcccccg ccaggcaccg gagcggaaga tcagcgccag cgggccgcgg
     7081 gcagcgcgcg gccggtgaag tcgaacagcg cctggttctc ccaggcgttg cccgaggccg
     7141 ggtcggcggg gtcccagccg ttgccgcggg tcgcgatcca cgccggctcc cagtagaaca
     7201 gcccgcgtcc gcgcccgccg ggcacggcgc cgaccacgcc ggagacggcg tcgagccagg
     7261 ccgcctggcc ctgcggcgtc ggggggaacg cggggggcaa ctggccggtg tccccgatga
     7321 ggttggggtg gccgtccgcg tcgcgcagcg tgaacgggta cgccgtctcg gccaggacga
     7381 cgtccttgcc gtaacgggcg gccgcgtccg ccaggttggc gcccaggtcg tcgagcgtgc
     7441 cgtgccagta cacgtagaac gacagcgcga tcacgtcgaa cggcacgccg cgcgccaccg
     7501 cctggtcgaa ccaccacacg gtgcccccgt tgtcgccgcc ctccgcgagg tgcagggcga
     7561 cctcggtgcc gggcgaggcg tccgcgacgg cacgggagcc cgcgcgcagc aggccggcca
     7621 gggcgttcca gtcgtcggtc gagccctcgg gccacagcag cccgtggttg atctcgttgc
     7681 cggtctgcgc catcgcggcc ggggtgccct gggcgcgcag cgcgccgagg acatcggacg
     7741 tgtggtcgta gagggcctgt tcgagcgcgc tgccgcgcag tcccgtccac gcggcgggct
     7801 tgcgctgctg ccccgggtcg gcccagctgt ccgagtagtg gaagtcgacc agcaggcgca
     7861 tgccgagggc atgcgcccgc cgggccatgg tcaggacctg cgccgtgccg tggtagccgt
     7921 cgggcgagtc gacccacacc ttgagcctga cccagttggc gccgccgtcc cgcaggatgc
     7981 ggaccgggtc gccgacggtg ccgtcggcgt accggaagac ggcgcccagg tcctccgcct
     8041 tcgggacgga ggagaggtcg accccccgca cgggcggcgc cgcggcgtgc gcggcgccgc
     8101 ccggcaggga ggcgaggacg gcggccggcc agggcggccg ttgtggcgcg gagcagtgtg
     8161 cgtctgtgca tcatgggtcc cttccatgag tgacaggact tcacccgcgg ggcgtcaccg
     8221 gtccaggcgc accacccggg tggcacccgc gggcacggcg agcgggccgt cggcgcgggt
     8281 gccggtgagc agttcggtgc cggaggcggg cagggccacc tcgtggtcct cggccgtgtg
     8341 gttgatcgcg aacaggaagt cgccgccggg gcccgcgcgc ctgaccacct cgacgtcgcg
     8401 gggcaggtcg tcgcgcgggt cgagaccggc gtccttcccc gccgcggcca ggacctggcg
     8461 cagcccctcc tggcccacgc gggccgagac gtaccaggcg gtgccgtcac cgagccggtg
     8521 gcgggtgatc gcgggcccgc cggccgcggg tccgtcggcg taggtccaca ccgtttccgc
     8581 gcccgtggtg cggacggtct ccgcccagac gtcggcggtc gcggtggcgc cctcagggcc
     8641 tgccagccgc accgtctcgc ccgcgcgcag cggctggaac tcctcgacga ccaggccgag
     8701 gacgtcgcgc agcgcgccgg ggtgcggtcc cgggtgcacg gcgtcgtgct cgtcgacgat
     8761 gcccgagaag caggacacca ccagcgtgcc gccgccctcg acgtagcggc gcaggttctc
     8821 gcccgcggcc cgggtggcga ggtagagggc cgggacgacg accagcggcc ggccggcgag
     8881 gtcaccggac tcggggtgcg cgaagtcgac ggtcaggtgc tggtcgaaca gcgcggcgta
     8941 ccacgcctcc gcgcgttccc tggtgcccag gtcgtcgctc ggccgccact ccagcttgtg
     9001 cgcccaccag gactcccagt cccacacgac ggccgcgtcg gccaccgtgc gggtgtccat
     9061 gatgtccgcc aacgcgtcga tgtgcccgcc gagttcgacg acctcgcgcc acacacggct
     9121 gtcggtgccc gcgtgcggca ccatcgccga gtggaatttc tccgcgccgg cgcgtgaggc
     9181 gcgccactgg aagaacatcg cgccctgcga gccgcgggcg acgtgcgcga ggctgttgcg
     9241 cgccatctcg cccggccgct tggcgatgcc gcgcggctgc cagttgacgg cgctggtgga
     9301 gtgttcgagc aggagccagg ggcggccggc gccgacggaa cgggtgaggt cggcggcgaa
     9361 cgagaggttg atgtggttcc tgtcgtcctc ggccaccagg tagtggtcgt tggtgacgat
     9421 gtcgacctcg cggccccact tccagtagtc aatggtgtcg cactgggtgg gcgagaccat
     9481 gaagttggtg gtgaccggca cgccggggga cagttcgtgc agcaggtcgc gctcggcgcg
     9541 gaagttctcc agcgcgctgt cggaggcgaa gcgcgcgaag tccaggcgct gcgcggggtt
     9601 ggccgtggtg ggggtgagcc tgggcggctg cacctcgtcc cactcgccgt acgcctggcc
     9661 ccagaaggcc gtgccccagg ccgcgttgag cgcgtcgagg gtgccgtacc gctcggcgag
     9721 ccagcgcctg aagtgggcgg cggagacgtc gcagtagcac tcaaggacgg gcactccgta
     9781 ctcgttgtgc acgtgccaca gcgcgagcgc cgggtggtcg ccgtaccgct cgccgagcgc
     9841 gcgggtgatg cgcgcggcgg cctcgcggta ggcgggcgag ctgtggcaga tcgccccgcg
     9901 cgagccgaac tccaggcgca cgccctcccg cgtgaccggc agcgcctcgg ggtgcgcgcg
     9961 gtagaaccac gccgggggga cgaccgtggg ggtggcgagg tcgatccgga tgccgccctc
    10021 gtgcagcagg tcgatgatcc ggtcgagcca gccgaagtcg tacacgccct cgcggggttc
    10081 gagccgggcc caggagaaga tcccgatgct gacgagggtc accccggctt cgcgcatcag
    10141 cgcgacatcc tccggccaca gctcctcggg ccactgctcg gggttgtagt ccccgccgaa
    10201 cgcgagtcgg tccatgcgca tctgtcgagc tgcctttcct gtgaacgtgc acaggcaaca
    10261 taaccgcaca gcaccgacca ttgacaaggg ttggatcggg cccttcctgt gcacgatccc
    10321 agccctcgcc ccggctctcg cagcggcgcc gtacagtcgt gcccgtgagc ccgacgaaca
    10381 acacgacccg gcgcag
//
