LOCUS       NZ_JAIONG010000040     10332 bp    DNA     linear   CON 22-NOV-2024
DEFINITION  Bacillus amyloliquefaciens strain SN_Endo51 sequence40, whole genome
            shotgun sequence.
ACCESSION   NZ_JAIONG010000040
VERSION     NZ_JAIONG010000040.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus amyloliquefaciens
  ORGANISM  Bacillus amyloliquefaciens
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus; Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 18478)
  AUTHORS   Roy,M. and Dutta,T.K.
  TITLE     Community profile and biosynthetic gene analysis from end to end
            microbiomes of the medicinal mangrove Suaeda monoica Forssk. ex J.F.
            Gmel of Indian Sundarbans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18478)
  AUTHORS   Roy,M. and Dutta,T.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2021) Microbiology, Bose Institute, P-1/12, CIT
            Road Scheme VIIM, Kolkata, West Bengal 700054, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 30-MAY-2021
            Assembly Method                   :: SPAdes v. 25.05.2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020179775.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 08:35:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,314
            CDSs (total)                      :: 4,259
            Genes (coding)                    :: 4,097
            CDSs (with protein)               :: 4,097
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 91 of 162
            Pseudo Genes (incomplete)         :: 72 of 162
            Pseudo Genes (internal stop)      :: 30 of 162
            Pseudo Genes (multiple problems)  :: 28 of 162
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:57:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8146
            Orig. end                         :: 18478
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAIONG010000040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10332
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10332
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10332
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5360](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5360
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            916..1098
                     /locus_tag="K8B35_RS20625"
     CDS             916..1098
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K8B35_RS20625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_121984832.1"
                     /transl_table=11
                     /translation="MKKIVAVAVLISIMALFFSVFHNKHDEKRLAGRAEYAIQGTHYTA
                     LADGQFETQGRAEFA"
     gene            complement(1517..1705)
                     /locus_tag="K8B35_RS20630"
     CDS             complement(1517..1705)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016938739.1"
                     /locus_tag="K8B35_RS20630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_121984833.1"
                     /transl_table=11
                     /translation="MNDFERKVFRIIFNMTHFGKNPTLEELKRKTGKDERAIREAIKNL
                     MRQRLLKWDKKKNRWMF"
     gene            complement(1881..2330)
                     /locus_tag="K8B35_RS20635"
     CDS             complement(1881..2330)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014304257.1"
                     /locus_tag="K8B35_RS20635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_121984834.1"
                     /transl_table=11
                     /translation="MKSKKIFKSLIIVFALLISGIGFMGFSSPQKAEAKDWLHTYKSGY
                     FTNYVIFTQHPKFNAKTTKKFSLEDGRIKSSSKGKGTYKVYIQKQVNSKGAWKTIKTFN
                     AKKNGVTYFETPWLKKGDSYRFKMVNQGSKKRVNYNASWIDYASR"
     misc_feature    complement(2429..2447)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.41"
                     /tool="antismash"
     assembly_gap    3296..3390
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3391..3584)
                     /locus_tag="K8B35_RS20640"
     CDS             complement(<3391..3584)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K8B35_RS20640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_224988983.1"
                     /transl_table=11
                     /translation="MWNRLGAGFLCVLGRYGTPWMRLKAKRPAFMGRSGEPSAARRAVN
                     SPWLIEAAGAAGFRSRPGAG"
     gene            3815..4132
                     /locus_tag="K8B35_RS20645"
     CDS             3815..4132
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012118696.1"
                     /locus_tag="K8B35_RS20645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_198698516.1"
                     /transl_table=11
                     /translation="MNSQLRKGVMELCVLAMIRKEDLYGYELIRSLSEKFPVKGETIYP
                     VLKRLTNGGFLSTYIGESSQGPSRKYYKITPEGERQYQLLHAEWSEFSSVVNEIFEEEE
                     Y"
     gene            4132..4671
                     /locus_tag="K8B35_RS20650"
     CDS             4132..4671
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF046861.1"
                     /locus_tag="K8B35_RS20650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1700 domain-containing protein"
                     /protein_id="WP_121984792.1"
                     /transl_table=11
                     /translation="MSKNEYLKRLRSSIKQLPKDDQKEILQDIEEAFIIGLSSGKSEYE
                     ISTHLGNPQTLAKTLIADSIIQNSKNNHSVQHFFRIFMRVIGISFFNFFFTLCPFILIA
                     GILVSLFATSIMSVGAALFVVPRSFFNALFVLGGGVILFQISIDLIKVFIKFFLKYINW
                     TVKLVRRKKIDEKMAL"
     gene            5001..5360
                     /locus_tag="K8B35_RS20655"
     CDS             5001..5360
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="K8B35_RS20655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_224988985.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 3.9e-09,
                     bitscore: 27.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKVLASIVLGIGLLGGQAAFASQSNDVNHGEVNLDETRDLMSA
                     MKLDKSTPGGGTWYHGFEKGQVHSNYNHKKKTHKSSAAAGARFYETEWNTKNQGYTYAS
                     VYEALFGNKAFWDTK"
     gene            5457..7622
                     /locus_tag="K8B35_RS20660"
     CDS             5457..7622
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01654.1"
                     /locus_tag="K8B35_RS20660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_224988987.1"
                     /transl_table=11
                     /translation="MKKTIYILFILVLLSMNMSSYHYTLNKQIYYTLFSNKEALIINYT
                     SEFKSEDFVKRLVAFSKENKVNISQYNFLDEKSLNIYSTSPENEPSIHNGNKLVSNFLR
                     GHSEQKMIGSITFPLSTWKIRYFDFNQIKNIGIGSKFYISSNDEKVLEKAQNLFADYGE
                     VSYEDITENTAFMFNKTLLMLVLLSMLILFIGVLYFVIKNRKRLLLQKLWGYSKVKTLL
                     SYPAMFLKPLIIIIFCGAIIVALLAFSFSLNDWLAAYLRIYIRNAVIGALIMMVYTVIV
                     TLILYNNSDISGSIKGSTPIKKFQWLSVGFKIVVTILLFNIVASSLSNVFDLKQQLDNQ
                     SYWKKTQNTYKTSFANTGIDYSDLKLDREKNVRVRALYHQLEKHKNAFVIDAHNYAVLR
                     KDGKKPVYFYTLNTTKKNEIYSPSGRSITISPNYLKVNPIIGANGEAISQKQLRADSNT
                     LNLLVPKKYEKYKEKIIAAYRDHFFFKKVELDNMYNKEINQPVNTLKKDELKINIIYTK
                     NRQEYFTFNSEFGDAGNKNNIVDPIAAIFTDNVDPSVISAYSTSCLFFSDSSKGMAYDR
                     IVPYLEKTNTRDLINSSISVYQELSDGIARIQNQFLQNLIGLTITVILSIAFLVAYIWS
                     YYSANAYRLYLKELFGYSYWARNKNLIIFSLLTNCLIGLGCSIYYKIFELTIFIALFIV
                     IELLILYFLSVYLNRRNMNRILKGDRI"
     gene            7619..8239
                     /locus_tag="K8B35_RS20665"
     CDS             7619..8239
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328395.1"
                     /locus_tag="K8B35_RS20665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_224988989.1"
                     /transl_table=11
                     /translation="MITLNNISKTFDEKPVLSNFSLQIKENEFVSIVGKSGSGKTTLLN
                     IIGLLDTPHSGDIEILGFRNPKSNDVMKLRRENLGYIFQNYVLMDNETVLTNLLLSTAY
                     AKDFKKEKLTEALEMVGLDKSFLKKKIYQLSGGEQQRVAIARIILKPCDIILADEPTGN
                     LDEYNKSIILSLFHQLKGMGKTIICVTHDQEIADGSDRVINLS"
     gene            8253..8468
                     /locus_tag="K8B35_RS20670"
     CDS             8253..8468
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328394.1"
                     /locus_tag="K8B35_RS20670"
                     /note="Built to rescue LMOF2365_14255 during structural
                     annotation change regression; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="bacteriocin-like WGxF protein"
                     /protein_id="WP_121984796.1"
                     /transl_table=11
                     /translation="MKHIVFALSASILVVIAGVVHRILFRVFGFPFDQAIFFWGGFVVV
                     YFILALLASLLFRSIGSNNRSSRYEA"
     gene            8777..8875
                     /locus_tag="K8B35_RS21770"
     CDS             8777..8875
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF028245.5"
                     /locus_tag="K8B35_RS21770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="putative holin-like toxin"
                     /protein_id="WP_224988991.1"
                     /transl_table=11
                     /translation="MAIDSAIQLMLTFGLLIVGILTLAHNFSNKKK"
     gene            complement(9676..9864)
                     /locus_tag="K8B35_RS20680"
     CDS             complement(9676..9864)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K8B35_RS20680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_224988994.1"
                     /transl_table=11
                     /translation="MLFCRRRIGAEGRGSPSGGPFLDQDTKKADVWPAPESCRLRSLDE
                     QRRFHGEGVASALTIAP"
ORIGIN
        1 gcagccgaac ctttcagatt tattatcaga aattgagaat aatcagtctc gtatcaaagg
       61 aatgctggac tactatttta atttctttaa aggacaattt gaattttcta ggaagaatta
      121 tattcaagcg atacaatatt ataaacgagc agaattgaag ctagatctaa ttcctgaaga
      181 agagaaaatt gaaaaagcag aatttcatta taaagtggca gctgcttatt atagaatcaa
      241 gcagaatatg ttttcgctgc atcatgcaga gatcgcttta aatatgttcg aagccaataa
      301 actctatagt acaagtacca taagttgtga aatgatgtta ggagcaaata agcttgatct
      361 tcttcgattt gatgaagcag aggcccatta tacaaatgcc atgaagtttg ctgaattgac
      421 aaaggataat tattcaagag gattggcttg tcataatatt gggttaagct atgcgcttca
      481 aaacaaaagt gacttagctg aagaatattt ccgaaaagtc cttgaaatac cagaatatta
      541 tgaaactgaa cgtgggttaa ggactgattt tgaattgact tttgtcttgt ataagaatgg
      601 taaaatagaa gaggcacaaa agcttttagt aaagggcatc aagaaagctg aaaaagaagg
      661 tgaagcagag tatttggcga aatttaatat tgtccatgct ctttacggtg gcttagaggc
      721 acaaaagcgt gatgatctta ttcagagagc attcaaatat cttgaagaaa agcagctatg
      781 gacagatatt gaagaattga gccaagatat cgcggaacat tttcgcataa tcaaggatta
      841 tgcgaatact gtattatatc tggataaggt gatctacgcg aagaacaaaa ttctcagcgt
      901 atcggaggaa ttaatgtgaa gaaaatagtg gctgtcgcag ttctcatctc aattatggct
      961 ttatttttct ctgtatttca taataagcat gatgagaaac ggttggctgg cagggccgaa
     1021 tatgcaatac agggaacaca ttatacggct ttagccgatg gacagtttga aacccaaggt
     1081 agagctgaat ttgcatgatc ctttccctcc gacgaatgtt cggagggttt tacgtatgac
     1141 aaattttata tttatcatca aaaaatgatt ctaacgcaaa aacaaattca aaggggagaa
     1201 tgcctgtatg aattttagaa gaggcgtctt tatcattgta tcgactatcg tattaatgtc
     1261 agggatcggt ctaactgcat cggccgtttc attagatgga tcatatcaag tttcgtctga
     1321 tcgtaatcaa acctaagaag aagagtgcct gtatgaatct aaaaaaagag cgtcttcatc
     1381 gtcctgtaag tgggttgggt atgaacttag tagagaaaca aacacgagat acaacccagg
     1441 tagcgtctga tcgattttga cttgaacaac ttacgccttt tcttcggaaa aggtgtttta
     1501 tttttattat aaaatctcaa aacatccatc tgttcttttt cttatcccat ttcagcaaac
     1561 gctgtctcat gagattctta attgcttctc gtatagcgcg ttcatcttta ccagtttttc
     1621 tcttcaattc ttctaaggtg gggtttttcc caaagtgagt catgttaaaa atgatccgga
     1681 acactttcct ctcaaagtca ttcattcaaa ctgcctcctc gagtagataa taccagaaca
     1741 gacctaacta tggggaagcc agacatctga atgcatgggt ccgatgaagg agatccgtag
     1801 ctgttggctg ttattagggc aaataaaaag ctactgcagc tgcagtagct aaaaaagggt
     1861 ttataagccg caaaatcaaa ttagcgggaa gcgtaatcaa tccagcttgc gttataattc
     1921 acacgttttt tagaaccctg attgaccatt ttaaatctat atgaatctcc tttttttaac
     1981 caaggtgttt caaaataagt tactccattt ttctttgcat taaaggtttt aattgttttc
     2041 caagcgcctt tgctgttgac ttgtttttgg atgtatactt tatacgtgcc tttcccttta
     2101 ctgctagact taattctacc atcttccaag ctgaattttt ttgttgtttt tgcgttgaat
     2161 ttagggtgct gtgtaaagat cacatagtta gtgaaatacc cactcttata agtatgcaac
     2221 cagtctttag cttctgcttt ttgtggagaa gagaagccca taaaaccaat gccactaatt
     2281 aaaagtgcaa aaacaatgat taatgactta aaaatctttt tacttttcat ggagaaaatc
     2341 cttccttcct gtaactattt accctattta tgttaatggc aatataaaca atcataaacc
     2401 cgtctaactt actattttga atgtcagaag aggtgggttt aacctaaaaa tgactatatt
     2461 actaatactt atgattcatt attttgtttg gtttgggaat aaagttgttt tatgatgatg
     2521 cggaatatat ctgtttttat atcaattgtc atgagatgat tttacgtcta gttctatgtg
     2581 tatgggcggc aaagtaaagg atatataaga ttcattatac cggaagccca agcctgttaa
     2641 aatgcggtgg gtttatctcc ctcagcgcag ccggtcttct tccagtggtg tatcgaggat
     2701 gatagtcgcg gatctgatct ccttcagtag ccagggcgtc ccgttgtctg gacgcagggg
     2761 cataataagg ccgatatctg gctgcctgcg gacccgcctg ccccgcaact tctatgatcc
     2821 gcgcagattt taaggtgaag gcgctaccga tacactaccg atcgctccgt aaacgcggga
     2881 cgttttcgac ttcagatgct ctcctgccgg cgccgcatcg gcaccgacgg ccagggatag
     2941 ggcggcccgt agcttgccct ggacacaaaa agccaacgcc tggtcggctc cggaatccga
     3001 cagccccgca gcttcgatga gccgcagaga tttcatgagg cggcgctgcc ggtgcatccc
     3061 tgatcgcccc gtaaacgcgg ggcgttttgc cttcagacgc tctcctgctg gcgccgtatt
     3121 agggccgtcg gatgcggata tccttcagct gcagccaggg cggcccgtag cttgccctgg
     3181 acacaaaaag ccggcgcctg gtcggctccg gaatccggca gcccccgcag cttcgatgag
     3241 ccgcagagat ttcatggcgc ggcgcgctgc agatgctctc ccgatcgccc cgtaannnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccggcgcctg gtcggctccg gaatccggca
     3421 gcccccgcag cttcgatgag ccacggagag ttcacggcgc ggcgcgctgc tgatggctct
     3481 cccgatcgcc ccataaacgc ggggcgtttt gccttcagcc gcatccaggg cgtcccgtat
     3541 ctgcccagga cacataaaaa gccggcgcca agccgattcc acaatcctgc tgcctcgcag
     3601 cttcgatgag ccgcagagat ttcatggtgc ggcggagcca gcgcacgcct gatagcccca
     3661 aacgcagagt gattttcgcc ttctacacgc ggtctgctcg tctgtggtat aaattggtga
     3721 aggatcgtat ctataattac ctttaaaaca tggttgcata tataactagt gtgttttaca
     3781 ctatagtata aataagaaaa caggatggtt gattatgaat agccaactta gaaaaggcgt
     3841 aatggaactg tgtgtcttgg caatgataag aaaagaagat ttatatggtt acgaattgat
     3901 aaggagtctc agcgaaaaat ttccggtaaa aggtgaaaca atctatcctg tcctgaaaag
     3961 actgactaat ggcggcttct tatccactta tattggtgaa tcatcccaag gcccaagtag
     4021 gaagtattat aaaataacgc ctgaaggaga gcgtcaatat cagctcttac acgccgaatg
     4081 gtcagagttt tccagtgtag tgaatgaaat atttgaggag gaagagtatt aatgtcaaaa
     4141 aacgaatatt taaaaaggct ccgatcatct ataaaacagt taccaaagga tgatcaaaag
     4201 gaaatattgc aagatattga agaagcattt attattgggc tgtcttctgg aaaatcagaa
     4261 tacgagatct ccacgcatct aggaaaccca caaacactag ctaaaaccct tatagcagac
     4321 tctattattc aaaactctaa gaacaatcac tcagtacagc atttctttcg catcttcatg
     4381 cgagtgatag gcatcagttt ctttaatttt ttctttacac tttgtccctt catactcatt
     4441 gctggcattt tggtatcgct ttttgcaaca agcataatgt cagttggcgc agcacttttt
     4501 gttgttcctc gttcattttt taatgccctt ttcgtcttag gtggcggggt aattcttttt
     4561 cagatatcaa ttgatctaat aaaggtgttt atcaaattct ttttgaaata cattaactgg
     4621 accgtcaagt tagtaaggag gaagaaaatc gatgagaaaa tggctttata gcgttgggtt
     4681 aacacttatg ataattggtc ttatcggaag catttttttt ggtttttctg gcctctggta
     4741 ctaacccctc agacagacta atcaaagtaa aagagatagc tgttatttat aattccacca
     4801 ttaaagaata cacaccaagc tagcattcta tcggcattag ataacaaaca ttatgagcct
     4861 attgtgttcc actatgtcca ttttccgaat aaaaacagga atgttgtaag aatatcaaaa
     4921 tttaagttgt gaatccttac ccaatatggt aattttgatt tgtaatacca aataaaccct
     4981 aattggaagg agagatttca atgaaaaaga aggttcttgc ttcaattgta ttaggtatag
     5041 gtttactagg aggacaagct gcttttgcta gtcagtcgaa cgatgtcaat cacggggaag
     5101 ttaatttgga cgaaacaaga gatttaatga gcgccatgaa gcttgataaa tcaacaccgg
     5161 gcggtggtac gtggtatcat ggcttcgaaa aaggccaagt tcattccaat tacaatcata
     5221 agaaaaagac tcacaaatcg agtgctgcgg ctggagcaag attctatgaa actgaatgga
     5281 atacaaaaaa tcaagggtat acttacgctt ctgtatatga agcccttttc ggaaacaagg
     5341 ctttttggga tacaaaataa agaaaacgat agcaaagtga tttttaaatt tttcttccgg
     5401 tgtggtcaaa cttgaataca agtcagcacc ggatttcttt ttatggaggc tgtccaatga
     5461 agaaaacaat ctacatattg ttcattttgg ttttgctctc aatgaatatg tccagctatc
     5521 attacactct taacaaacaa atctattaca cgttattctc aaataaagaa gcattgataa
     5581 tcaattatac ttcagaattt aaaagtgagg actttgtaaa gagattggtc gccttctcca
     5641 aagaaaacaa agtgaacatt tctcaataca atttcttgga tgagaagagc ttaaacatct
     5701 attctacaag ccctgaaaat gaaccatcaa tacataacgg caataaactt gtgtcaaatt
     5761 ttctaagagg ccattcagag cagaagatga tcggcagcat tacttttcca ctttcgacat
     5821 ggaaaatcag atattttgat ttcaatcaaa taaagaatat aggcatagga agcaaatttt
     5881 atatttcgtc taacgatgaa aaggtattgg aaaaagcaca aaatcttttt gctgactatg
     5941 gcgaagtaag ttatgaggac atcactgaga atactgcttt tatgtttaac aaaactttgc
     6001 ttatgctagt gttgctctct atgctgattt tgtttatagg tgtcctttat tttgtaatta
     6061 aaaatcgcaa acggcttctg ctgcaaaagt tatgggggta ttccaaagtt aaaacactgc
     6121 tttcttaccc agcaatgttt ttgaagcctc taattataat catattttgc ggcgcaatta
     6181 ttgtggcttt gctagccttt tctttcagtt taaatgactg gttggctgca tatcttagaa
     6241 tttacattag aaacgcggtg attggtgcgc ttatcatgat ggtatacacc gtcattgtta
     6301 ctttaatctt gtataacaac agcgacatta gtggctcgat caaaggcagc acaccgatta
     6361 aaaaattcca atggttatct gtaggcttta aaattgttgt cacgatattg cttttcaata
     6421 ttgttgcgtc ctcactttct aacgtattcg atctcaaaca gcagcttgat aatcaatcgt
     6481 actggaaaaa gacccaaaac acttataaga caagctttgc aaataccggg atagactata
     6541 gtgatttaaa attagacagg gagaaaaatg ttagagttcg cgcgttgtat catcaattag
     6601 agaagcataa aaatgcgttc gtcattgatg cacataacta tgctgtacta aggaaagatg
     6661 gaaaaaaacc tgtttatttt tacaccttaa atacgacaaa gaaaaatgaa atttactctc
     6721 cttctggacg aagcattaca atcagtccta attatttaaa ggtgaatccc attatcggcg
     6781 ctaatgggga agccataagc caaaagcaat taagggctga ttcaaataca ttgaacctgc
     6841 ttgttccaaa aaagtatgaa aaatataaag aaaaaataat agctgcatac agggatcact
     6901 ttttctttaa aaaagttgaa ttagataata tgtataacaa ggagattaat caacctgtaa
     6961 acaccctcaa aaaagatgaa ttgaaaatca acatcattta tactaaaaat aggcaggagt
     7021 atttcacctt taatagtgag tttggggatg ccggcaataa aaataacatt gttgatccaa
     7081 tagctgcaat tttcacggac aacgttgatc catctgttat aagtgcttac tcaacctcat
     7141 gcttattctt ttcagattct tcaaaaggca tggcgtatga ccgcatagtt ccttatttgg
     7201 agaagaccaa tacgagagat ctaattaatt catcaatttc ggtttatcaa gaactaagcg
     7261 acggtatagc aagaattcag aatcaattct tgcagaattt aattggacta acaataacag
     7321 ttatcctctc catagccttt cttgttgctt atatttggtc ctattacagc gcaaatgcat
     7381 atcggctgta ccttaaagag ctttttggat attcatattg ggccagaaat aaaaatttaa
     7441 tcatcttttc ccttttaacc aattgcctta taggtttagg atgcagtatt tattacaaaa
     7501 tcttcgaatt aacaattttc atcgcgctgt ttattgtaat cgagctgctt attctttatt
     7561 ttctgagtgt ctatttaaac aggagaaaca tgaataggat cttaaaagga gatagaatat
     7621 gattacactc aacaacatat caaagacgtt tgatgaaaaa cctgttcttt cgaatttctc
     7681 tcttcagata aaagaaaatg aatttgtttc aattgttggt aagagtggtt caggaaagac
     7741 aacccttcta aatatcatag gtcttcttga tactccacac tcaggagata ttgaaatttt
     7801 aggttttcga aaccctaaat ctaacgacgt gatgaaacta cggagagaga acttagggta
     7861 tatctttcaa aattacgtgc ttatggacaa cgaaacagta ttaacaaact tactgctttc
     7921 tacggcatat gcaaaagatt tcaagaaaga aaagttgaca gaagcattag aaatggttgg
     7981 gcttgataaa agctttttaa agaaaaagat ctatcagctg agtggtggag agcagcaacg
     8041 tgtggccatc gcaagaataa ttttgaagcc ttgtgatatt atcctggctg atgaaccgac
     8101 aggaaattta gacgagtata acaaaagcat tattttgtct ttattccatc agttaaaagg
     8161 tatggggaaa acgattattt gtgttacaca tgatcaagaa atcgccgatg gttctgacag
     8221 agttattaat ctttcataag gagaggttta aattgaaaca cattgttttt gcattatcag
     8281 caagtattct ggtcgttatt gccggagtgg tacacagaat actctttaga gtgtttggct
     8341 ttccatttga tcaggctatc ttcttttggg gcggattcgt tgtagtttat tttatattag
     8401 cgttgctagc ttctctgctc tttagaagta taggttcaaa taacagaagt agcaggtatg
     8461 aggcttaaaa tcagcgatca acttaggaac gtatttctag gagaatgaat aaaacaagga
     8521 gaccagccga agatgccggt cttttttata tggagtggaa ttagccaggt aaaggatgat
     8581 catctatttg tacatctaaa gtagcaacaa tatgattata gacggcctgc gagagttttg
     8641 gattcgactt tttttaaaaa cgatctcagt gtgttgtgtt tttgtgtcag ttcatagtaa
     8701 agtataaata tcagtagaac ggcttttgca ggggtattgg ctcacctcca ttcttggaaa
     8761 gaaaagaggt gagaatatgg ctatagacag tgcaatccaa ttaatgctta ccttcgggtt
     8821 attaattgta ggaattctta cattagcaca caatttctca aataaaaaaa agtaagaacg
     8881 cctctgaccg tcacatcatg cgttctcact cattaatttg cttttaggta aaagccgact
     8941 cccttacagg agctattcta ttgtagatcg gagatgttag tagcatctct gatcttttta
     9001 ttttgcgtta ttattgacaa acgtattcac tttctgattg tcataaaaca taacgcgtat
     9061 ataacaactc atacctgatt atattgtgat tgtatctttt tattgaccga aaatcaacta
     9121 caaggaaaga ccgactttgt ttgctcaggg ataagcaacc ttcttttctc ctgagcaagc
     9181 aactgttttg ctctaaggat atagattgtg attcgtcgat gatttagccc tcatctcaag
     9241 cagttaggaa acgaagagta atgacactag caatacatga ttctctatgg cagtttttta
     9301 tgaagacggt gtttgataat aggagatgac tccaattatc cctaaaatta tcataacagc
     9361 tacaattgca aggatgatcc atactactat ttttatgctt tttgacatgt tatgtacctc
     9421 cattttaatt taattcaggt acgatccgtt ccattaatgt ttccaaaatg agtttaacac
     9481 aattttccaa ataaaaacac ggtagtatac tcggtttcat caagggcaga aggaatccat
     9541 aaccggccag gtaccgcctt ttttgtggcc agggcgggca acgggccgcc ctggctgtag
     9601 ctaaaggaaa tccgcgtccg ttggttccga tacagcgccg gcaggagaac gtttgaaggc
     9661 gaaaacgccc catgtttacg gagcgatcgt gagagcactg gcaacgcctt cgccgtgaaa
     9721 tcttcgctgc tcatcgaggc tgcggaggcg acaagattcc ggagccggcc agacgtcggc
     9781 tttttttgtg tcctggtcaa gaaacgggcc gcctgaagga gatccgcgtc cttcggcccc
     9841 gatacggcgc cgacagaaga gcatctaagg cgaaaacgcc ccgcgtttac aaagcgatcg
     9901 ggagtgtacc ggcagcgtcg cgccgtgaaa tctccgcagc tcatcgaagc tgcgggcaac
     9961 cggattccgg agccgaccag acgctggctt tttttgtgtc ctggtcaaga aacgggccgc
    10021 ctgaaggaga tccgtgtccg tcggccccga tacggcgcca gcaggagaac gtctgaagga
    10081 gaaaacgccc cgcgtttacg ggggcgatca gggctgcatc agcagcgcgc cgcgccgtga
    10141 aatctccgcg gctcatcgga gctgtggggc atggggattc cgaaaccggc cggtcaggcg
    10201 ccggcttttt ttgtggccag ggcgggcaac gggccgccct ggctgtagct aaaggaaatc
    10261 cgcggccatc ggcctcgata cggcgccagc aggagaacgt ctgaaggaga aaacgccccg
    10321 cgtttacgga gc
//
