LOCUS       NZ_JAIONG010000010     13079 bp    DNA     linear   CON 22-NOV-2024
DEFINITION  Bacillus amyloliquefaciens strain SN_Endo51 sequence10, whole genome
            shotgun sequence.
ACCESSION   NZ_JAIONG010000010
VERSION     NZ_JAIONG010000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus amyloliquefaciens
  ORGANISM  Bacillus amyloliquefaciens
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus; Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 138453)
  AUTHORS   Roy,M. and Dutta,T.K.
  TITLE     Community profile and biosynthetic gene analysis from end to end
            microbiomes of the medicinal mangrove Suaeda monoica Forssk. ex J.F.
            Gmel of Indian Sundarbans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 138453)
  AUTHORS   Roy,M. and Dutta,T.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2021) Microbiology, Bose Institute, P-1/12, CIT
            Road Scheme VIIM, Kolkata, West Bengal 700054, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 30-MAY-2021
            Assembly Method                   :: SPAdes v. 25.05.2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020179775.1-RS_2024_11_20
            Annotation Date                   :: 11/20/2024 08:35:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,314
            CDSs (total)                      :: 4,259
            Genes (coding)                    :: 4,097
            CDSs (with protein)               :: 4,097
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 46
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 91 of 162
            Pseudo Genes (incomplete)         :: 72 of 162
            Pseudo Genes (internal stop)      :: 30 of 162
            Pseudo Genes (multiple problems)  :: 28 of 162
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:57:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 44605
            Orig. end                         :: 57684
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAIONG010000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13079
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..13079
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13079
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:8079]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8079
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            468..1691
                     /locus_tag="K8B35_RS11480"
     CDS             468..1691
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012118857.1"
                     /locus_tag="K8B35_RS11480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="WP_224988561.1"
                     /transl_table=11
                     /translation="MKTLAAGAAVAAALSVGAVSDLPGAKWLHPAAAQAAETVFKQNHA
                     ASGFLAGRYDAQAMSPTMFNWSRESRFTSTADGALKWEKNVPATPQNGAGAAVDGDGTV
                     FIQSKDGKLTAYHPDGTVKWVTENLGTTYTLTPVLGTNGVIYLPSHDKKLYFIDKETGN
                     ILTSVPLSGAPSSDAAIGSDGTLYVSTLDNYIYAIKPTSPSTWTQKWKFKTNGVVGSAP
                     VLASNGTLYTATYNNIFYAINSGTGQVKWSKTTSNGFKGYPVIDRDGTVYAGNQDGNLY
                     AYTSTGAVKWTFPLNGFSSSSLAIDHNGNVYIGSGSGELFSISKTGNMNWSFYTDGPVR
                     TAPLIDADGNVYFGSDDKNVYAVDADGNEKWRYQTDSNVISSPVLAEDGTLYVGTYTKL
                     LAFGPKKS"
     misc_feature    514..528
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 20.59"
                     /tool="antismash"
     gene            complement(1722..3059)
                     /locus_tag="K8B35_RS11485"
     CDS             complement(1722..3059)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391865.2"
                     /locus_tag="K8B35_RS11485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_095352502.1"
                     /sec_met_domain="Aldedh (E-value: 5.1e-96, bitscore: 314.9,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDQHIKADIKRVFELQKKNQKTLRASTAGQRREKLQRFLDAVTAH
                     EEEIIEAIRRDVRKPYHEVKRAETQGTIKAIKDNMNNLDTWMAPKRVGSSVSPDADGVL
                     MYEPKGLTLILGPWNYPFMLTMAPLAAAIAAGNSAIVKLSDFTMETSHIAATVIREAFH
                     EEEVAIFEGEVDVATELLDQPFDHIFFTGSTNVGKIVMTAAAKHLSSVTLELGGKSPTI
                     IDSGYDLMDAAKKIAVGKFVNAGQTCIAPDYLLMKKEVQSTFTDLLQTIVNAGFMEDDH
                     TVDRNKFTQIVNDRNFNRVKALYDDAIEKGAKTAFGGVFDAHDRTISPTVLTNVTPDMK
                     IMQEEIFAPILPIMNYEDISEAVDYITERDKPLALYVFSHNQELIDYVLQHTTSGNASI
                     NDVVVHFTDVNLPFGGVNTSGIGSYHGVYGFKEFSHEKGVFIQASK"
     misc_feature    complement(3020..3033)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.31"
                     /tool="antismash"
     misc_feature    complement(3496..3513)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            complement(3535..5004)
                     /locus_tag="K8B35_RS11490"
     CDS             complement(3535..5004)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332544.1"
                     /locus_tag="K8B35_RS11490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_174747412.1"
                     /transl_table=11
                     /translation="MKRNFFTLQELKDSRELYESKPKPIGLIFVYLLIGILISAIAFSF
                     YGKKEVILKTTADIQYSKPNSNVTNEIAGKVKTVSFKQVDKVKKGDTLFTLDDTEIQTQ
                     RKASKADINDLKEQIALNKTLKKSIENDKNLFSEDDHWGFQDQYLSYKKQINLLETSEE
                     TVKTQKEKIDKHTKNVKNNLNKTKSKVTLKLQRISDLQNSIRRDKSFAKQDDKYSSYYN
                     NYLTSKQNAEQQLEDVEKMKDELDKSSYSEQKENLNGQIESLKQDMLVKLSEEKDELKQ
                     SLDEINSSIESDNTDIDSNDSEQTNYRLSIEQLKTNLLNQTAQKQTDLQTTLKAKEDDL
                     KMIDLQLEKYKIKAANDGYVNVLTNISPGDNLQAGTSILTIIPDKEKLKANIYIPSYEI
                     TNINEKDEVKFHLENTSQTIKGVITNISRDSISNQKGQKFYIAESTIANNEIVKDNGEK
                     IHLKNGMQTNVSIIVDTKRYIDYFLDVLHLH"
     gene            complement(5001..7184)
                     /locus_tag="K8B35_RS11495"
     CDS             complement(5001..7184)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014481436.1"
                     /locus_tag="K8B35_RS11495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_025650414.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 9.8e-45, bitscore:
                     144.7, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 4e-236, bitscore:
                     778.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFFKKYHCVKQMDEKDCGAACLSTIARQYGSKLSITYIRDIAGTD
                     TQGTSVLGMVKAAEQLGFSAKAVRAEHAAFDQSITLPAIAHIVIDQQLLHYVVIHKISK
                     EKIIIADPGKGLIKYKKDDFLKIWTGILILLTPTEAYEKQKEIRNISSDIFRLILQQKS
                     LIIHTFLASFIITIFGILGTFYFQTIIDQILPNGLSNTLHILSIGLLIMYLFKVFLIGF
                     RQYMMILLGQKLSITIMFGYFKHVLSLPMKFFSNRKVGEIISRFTDANKVIDAVVSASL
                     SILLDTIMLIMVGIFLYLQNATLFLITLAFVPLYILTVWIFIKPYANINNTEMENNAQL
                     TSKIVETLNGIETVKAYNAEYMMTFETEKRFVKYLRSAFKHGVIDNLQSSIKMFLDLAS
                     GALILWIGSTQVMKGNLTIGQLITYNALLTYFLNPLQNIIGLQSKFQSASVASKRLGEI
                     IEQDPEIKDNEKRITSISSITGPIVFNKINFRYGTRRLILKNIALQIQKGEKVAFVGES
                     GSGKSTLTKLLMKFYAAESGEILINGYHINDIHTNALRESISYIPQESHFIQGSILENL
                     LLGNSGSFTFEEIIEVCKQTKVHDFIDHLPMRYDTLIEENGSNLSGGQKQRLAIARALL
                     RKPNILIMDESTSNLDTTTEQGISKMIYDQTYNITTIIIAHRLSTIMNCDKIFVMENGE
                     IKESGSHHDLLMKKGKYYELWENQIPSKEMVTI"
     misc_feature    7169..7182
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.67"
                     /tool="antismash"
     gene            complement(7303..7563)
                     /locus_tag="K8B35_RS11500"
     CDS             complement(7303..7563)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K8B35_RS11500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_159353633.1"
                     /transl_table=11
                     /translation="MDFLYMLVETILWIIVTTIPSKRNLKFRRNLKSLKKQAWFCELKK
                     NYGPLFLMNSTIQDKIIEYNENNDLETYKEEIENLAKSIHV"
     misc_feature    complement(7606..7624)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 24.12"
                     /tool="antismash"
     misc_feature    complement(7778..7793)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.12"
                     /tool="antismash"
     gene            7858..8079
                     /locus_tag="K8B35_RS11505"
     CDS             7858..8079
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K8B35_RS11505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_025650416.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.4e-09,
                     bitscore: 30.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKQNNRFESLTVEEAEMIDGGGYGSYLLKGVGTGIAVGAALGGP
                     GGAVLGANAGAIWGSAAYGVDALISGHK"
     gene            8121..8339
                     /locus_tag="K8B35_RS11510"
     CDS             8121..8339
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K8B35_RS11510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_025650417.1"
                     /transl_table=11
                     /translation="MLHNFEKSNNMIELSEQEMLDTDGGGWRDLAGAAAGAAVMGGTAM
                     ALGAAGGPVGFAAGIGLAILTRPGHAK"
     gene            complement(8884..9108)
                     /locus_tag="K8B35_RS11515"
     CDS             complement(8884..9108)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472512.1"
                     /locus_tag="K8B35_RS11515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_025650419.1"
                     /transl_table=11
                     /translation="MNNKVKVERLTLGLTQATLAEKLGITRQTVSLIEKGQYNPSLNLC
                     IKICNVFNKTLDDIFWPSDIKREDRENDR"
     misc_feature    complement(9204..9220)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.37"
                     /tool="antismash"
     misc_feature    9592..9605
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.03"
                     /tool="antismash"
     gene            complement(<9699..9938)
                     /locus_tag="K8B35_RS11520"
                     /pseudo=""
     CDS             complement(<9699..9938)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003326192.1"
                     /locus_tag="K8B35_RS11520"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="aldehyde dehydrogenase family protein"
                     /pseudo=""
                     /sec_met_domain="Aldedh (E-value: 1e-08, bitscore: 27.1,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDQHIKADIKRVFELQKKNQKTLRASTAEQRREKLQRFLDAVTAH
                     EEEIIEAIRRDVRKPYHEDGAETSSCPAGGCKENL"
     misc_feature    complement(9899..9912)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.31"
                     /tool="antismash"
     gene            10080..11237
                     /locus_tag="K8B35_RS11525"
     CDS             10080..11237
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012118855.1"
                     /locus_tag="K8B35_RS11525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_159353632.1"
                     /transl_table=11
                     /translation="MPDSVTTDIKQALLAMLGERDIRRVTMKDIAERSHVSRGTLYLYY
                     EDKYAILEDITEEMKDGLSEALYDSLKNKDVLHLHGGWQKVHPTLSFVDRHREFFQTMM
                     DRHKMPYFHFHAFLKDVFRHDVLLSPVNADFSPTEQDMYIHYRALYTYAIVLYWLNEEP
                     GASPEAISGQVWDLISQKRFYWLFGRRVPEQEEKKPADRRVTRTRQALQEAMIGLMAEK
                     KEYAAITISDIARQSNLRRATFYDHYANKEALLKTIIHQSCRDLIGLLTVKGEPADCSM
                     EEAEAALVRLFSALSDGLPLVHFLREGSGVPDVIPEMFRALQSFYLHQPLHIQAGKKLY
                     AYYVASMLAGLIQYRLHEGKDHAPDMLALEFLQFLDVKKYRVILL"
     gene            11482..12531
                     /locus_tag="K8B35_RS11530"
     CDS             11482..12531
                     /EC_number="1.14.19.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) delta12_cd03507"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389799.1"
                     /locus_tag="K8B35_RS11530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="fatty acid desaturase"
                     /protein_id="WP_172869785.1"
                     /sec_met_domain="delta12_cd03507 (E-value: 1.1e-103,
                     bitscore: 340.3, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINHAATQKQASLKKQVAAFSGADTKHSVIQLFNTFVPFFALWFL
                     AYFSLDVSYLLTLAFTVIAAGFLTRIFIIFHDCCHQSFFKQKKLNRLFGFISGVLTLFP
                     FLQWQRSHSIHHATSSNLDKRGTGDIWMMTVKEYNEASAWTKVRYRLYRNPFIMFILGP
                     IYVFLIKNRFNVKGARRKERWNTYFTNAAIVLLAAATCLLVGWENFLLVQGPIFLISGS
                     IGVWLFYVQHTFEDSYFEADEHWDYVQAAVEGSSFYKLPKLLQWLTGNIGYHHVHHLSP
                     KVPNYKLEAAHEGHEPLKNVPTITLKTSLESMKFRLWDEDEKQFVTFREARKTPLPQPV
                     PKEPLKKNA"
ORIGIN
        1 ttctccgctt gcggaaacgg ggccgaacgc gaatcgtttt aattccgcgg gcatgtgggc
       61 cgccgtcggc cagatctttg ttatatatgc ggtcccgctc attttgtata ttctcggtat
      121 tcgtgtcatg aaaatcatca tggccgtttt ttgcggaatc gggcttatca tttgcgcggc
      181 tgtcttgttg gtgacgttat tgaccgcaat ttccttaggg caggagctgt ctctttatta
      241 cggccttctc gtttggtcag gcgcggcttt tatcgtcaat gtggtgtggt atattgccgc
      301 gtttcgatca tccccgaagc atcagcaggc tatgtgacat ttgttctata aatctgccga
      361 aaatacaaat atttcatagg ttaattatgg tatgatcaaa gtgatatagc agcttttaat
      421 agaaacggga ggaactgaca ttgaaaaaaa agacagcttc attgcgaatg aaaacattgg
      481 cggccggcgc tgcggttgct gcggctttat ctgtgggagc ggtttctgat ctgccggggg
      541 caaaatggct gcatccggcg gctgctcaag ctgcggaaac ggtatttaaa caaaatcatg
      601 cggcatccgg tttcttggcg ggacggtatg acgcgcaggc gatgtctccg acgatgttca
      661 actggtcaag agaaagccgg tttacaagca ctgccgatgg cgcattaaaa tgggaaaaga
      721 atgttccggc cacccctcaa aacggtgcgg gagccgcggt tgacggggac ggcaccgtat
      781 ttattcaatc aaaagacggg aagctgacgg cgtatcatcc ggacggtact gtgaaatggg
      841 tgacggaaaa cctcgggacg acctatacgc tgactccggt gctcggaaca aacggtgtca
      901 tttatcttcc ttctcacgat aaaaaactat atttcatcga caaagaaacc ggaaacattt
      961 taacatcggt tccgctgagc ggagcgccta gttccgatgc ggctatcggc tctgacggca
     1021 cgctgtatgt gtcgacgctg gataactata tctatgcgat taaaccgacg tcgccttcga
     1081 catggacgca aaagtggaag tttaaaacaa acggcgtggt cggctccgct cccgtgttag
     1141 catcaaacgg cactctgtat acggcaacgt acaataatat cttttacgcg atcaattccg
     1201 gaacgggcca agtgaaatgg tcaaaaacga cgtccaacgg gtttaaaggt tatccggtta
     1261 tagatagaga cggcaccgtt tacgccggaa accaggacgg caacttatat gcctatacat
     1321 caacgggtgc tgtaaagtgg acgttcccgt taaacggttt ctccagctct tcattagcga
     1381 tcgaccataa cggcaatgtg tatatcggtt ccggaagcgg cgagctgttt tccatcagca
     1441 aaaccggtaa tatgaactgg tctttctata cggacgggcc tgtgagaacg gcgccgctga
     1501 ttgatgcgga tggcaatgtg tacttcggtt ctgacgataa aaatgtctat gcggttgacg
     1561 ctgacggtaa tgaaaaatgg cgctatcaaa cagacagcaa tgtcatttcc tccccggttc
     1621 tggctgaaga cggcacgctt tatgtcggca cttatacgaa actgctcgca ttcgggccaa
     1681 aaaaatcata aaaaaagaat ccgcatgcgc ggattctttt tttatttaga ggcttgaata
     1741 aatacgcctt tttcatgaga aaattcttta aagccgtaca caccgtgata tgaaccgatc
     1801 ccgctcgtat tgacaccgcc gaacggaagg ttgacgtcag tgaaatgaac gacgacatca
     1861 ttgatagaag cattgccgga tgtcgtatgc tgcagcacat aatcgatcag ctcctgatta
     1921 tggctgaata catacaaggc aagcggcttg tcccgttcag tgatgtaatc aacggcttca
     1981 ctgatatctt cgtaattcat aatcggcaga atcggtgcga agatttcttc ctgcatgatc
     2041 ttcatatcag gcgtgacgtt tgtcagcacg gtcggagaaa tcgtgcggtc atgcgcatcg
     2101 aatacgccgc cgaacgccgt cttcgcccct ttttcaatgg cgtcatcata cagggctttg
     2161 acacggttaa agttgcggtc attgacaatc tgcgtgaatt tgttgcggtc aaccgtatga
     2221 tcatcctcca taaaacctgc attgacaatg gtttgcagga gatctgtaaa ggtgctctgc
     2281 acttctttct tcataaggag atagtcagga gcgatacatg tttgccccgc attgacaaat
     2341 ttgccgacgg cgattttttt cgccgcgtcc atcaaatcat agccgctgtc gatgatcgtc
     2401 gggctttttc cgccaagctc aagcgtgacg gacgacaagt gcttcgccgc tgccgtcatg
     2461 acgattttac cgacattcgt gcttccggtg aagaaaatgt ggtcaaacgg ctggtcgagc
     2521 agttctgtgg cgacatcgac ctcaccttca aaaatggcga cttcttcttc gtgaaatgcc
     2581 tcgcgaataa ctgtcgcggc gatatggctc gtctccatcg taaaatcgga cagcttcaca
     2641 atcgcgctgt tgccggccgc aatggcagcc gcaagcggag ccatcgtcag cataaacgga
     2701 taattccatg ggccgagaat taaggtaagg ccttttggct cgtacattaa tacgccgtca
     2761 gcgtcggggc tgacggatga gccgacgcgt ttcggcgcca tccaggtgtc gagattgttc
     2821 atattatctt taatcgcctt gatcgttccc tgcgtttccg ctcttttcac ctcatgataa
     2881 ggcttgcgga cgtcccgtct gatcgcctcg atgatttctt cttcatgtgc ggtgacggca
     2941 tctaaaaagc gctgaagctt ttctctgcgc tgcccggctg ttgaagcacg cagcgttttt
     3001 tgattttttt tctgaagctc aaagacgcgt ttgatgtctg cttttatatg ctgatccatc
     3061 ggtcttcctc ctctggttgc ttttttgttt cttcagggcc tattataatg tgatgtgttt
     3121 ttgcggcaaa gacagcgtaa acacatctat acgttaccgt acaaattgcg gtgatctgtc
     3181 taatgaaagg agtgtctctt atgccggatt ctgtgacaac tgacatcaaa caagcgctct
     3241 tggttatgct tggggagcgg gatatccgcc aaaagtaaaa gtggtattgc tttgacatgg
     3301 cccggagtta acatccggga tttttacttt ttataagatg tgttttagga ataggcgggc
     3361 ggaaaaagtg tagcatttca gttggaggct gtgaagaatt attttgcaaa cgagatgacc
     3421 aagtgctgga tttcaaagaa gatcataagg tgtagcattt ggatttctga aaaaaatgcc
     3481 tcaaagcaaa tcttctttcc gctttgaggc atatgagaac tacttttaac aaatttagtg
     3541 caagtgcaat acatctagga aataatcaat gtatcttttt gtatcaacga ttattgatac
     3601 attagtctgc attccgtttt ttaagtgtat tttttcgccg ttatctttaa cgatttcatt
     3661 gtttgctatt gtgctttctg caatataaaa cttttggcct ttttgattac taatactgtc
     3721 ccggcttatg ttagtaatta cgcctttaat ggtttgactt gtattttcaa gatggaactt
     3781 aacctcatct ttttcattaa tatttgtgat ctcataactt ggaatgtaaa tgttagcttt
     3841 aagtttctct ttatcaggga taatagtcaa aatagacgtt ccagcttgta aattgtctcc
     3901 tggggatata tttgtcaata cgttaacata gccatcattt gccgctttta ttttatattt
     3961 ttctaattgt aaatctatca ttttcagatc atcttctttt gctttaagag tggtctgaag
     4021 gtctgtttgt ttttgggctg tctgattcag caagtttgtt ttaagctgtt caatagaaag
     4081 cctataattc gtttgctctg aatcattgga atcaatgtca gtattgtcac tttcaattga
     4141 gctattaatc tcatcaaggg attgttttaa ttcgtctttt tcttcactta gttttactaa
     4201 catatcttgt tttaaacttt caatttgtcc gttcagattc tctttctgct ctgaataact
     4261 gcttttatca agttcatctt tcatcttttc tacatcttcc agctgctgtt ctgcattttg
     4321 cttgctggtc aaataattat tatagtaaga gctgtattta tcatcctgct tggcaaaaga
     4381 cttatctctt ctaattgagt tttgcagatc agaaattctt tgaagtttta atgtaacctt
     4441 gcttttggtt ttgttcagat tatttttcac attctttgta tgtttgtcga ttttttcttt
     4501 ctgggttttt acagtttctt ctgatgtctc taacaagttt atttgttttt tataagagag
     4561 gtattgatct tgaaaacccc aatgatcatc ctcgctgaat aagtttttgt cattttcaat
     4621 actcttttta agagtcttgt ttaatgcgat ttgttctttt aaatcattaa tatcagcttt
     4681 agaggctttt ctttgtgttt gaatttcggt atcgtccaga gtaaacaaag tgtctccctt
     4741 tttcacctta tccacttgtt taaaagaaac tgtttttact ttacccgcta tttcatttgt
     4801 tacattgctg ttaggcttac tgtactgaat atcagccgtt gtttttagaa tgacttcttt
     4861 tttgccatag aaactgaaag caatggctga tattaatatg cctatgagta aatatacaaa
     4921 gattagaccg attggttttg gctttgattc ataaagttct ctgctgtctt tcaactcttg
     4981 taatgtgaaa aagtttcgtt tcatattgtc accatctctt ttgatggtat ttggttttcc
     5041 cacagttcat agtacttgcc ttttttcatt aacaaatcat gatgacttcc agactctttg
     5101 atttctccat tttccatgac aaatattttg tcacaattca tgattgtact taaacgatga
     5161 gcgatgataa tcgttgttat attataagtc tgatcatata tcatctttga tattccttgt
     5221 tcagtagtcg tatcaagatt gcttgtggat tcatccataa tgagaatgtt aggttttctc
     5281 aataatgctc ttgcaattgc cagtctttgc ttctgaccgc cagataagtt agatccgttt
     5341 tcttctataa gagtatcgta tctcattggt aaatgatcaa taaaatcgtg tactttcgtt
     5401 tgtttgcaaa cttctataat ttcctcaaag gtaaaacttc ctgaattccc aagcaaaagg
     5461 ttttcaagaa tagatccttg tataaaatgt gattcttgag gtatatagct tatgctttcc
     5521 cttaacgcgt tagtgtggat atcatttata tgatacccat tgattaaaat ctcgcctgac
     5581 tcggcagcat aaaacttcat taaaagctta gtcaaagtag atttccctga accgctttct
     5641 ccgacaaagg caactttttc ccctttttga atttgcagtg caatattttt taggatgagc
     5701 cttcgagttc cgtagcggaa attaatttta ttaaaaacaa tggggccggt tattgaagaa
     5761 attgatgtta ttcgtttctc attgtctttg atttctggat cttgctctat aatttcaccg
     5821 agtcttttag aagcgacaga agctgattga aacttggatt gaaggccgat aatgttttgt
     5881 aaaggattta aaaagtaggt taataaggca ttatacgtaa tgagctgacc gatggtgaga
     5941 ttgcctttca ttacttgggt agacccgatc cacagtatca atgcaccgct tgctaaatct
     6001 aaaaacattt ttatggaact ctggagatta tcaataactc catgtttaaa agctgaccgt
     6061 aagtatttca caaacctttt ttctgtttca aaagtcatca tatattcagc attgtaagcc
     6121 ttaactgttt caatgccgtt aagcgtttca acaattttcg atgttagctg ggcattgttt
     6181 tccatttcgg tgttatttat atttgcatag ggctttataa agatccaaac tgtcagaata
     6241 taaagtggta cgaaagctaa agtgatgaga aacaacgtag cattctgcag gtacagaaat
     6301 ataccgacca taataagcat aatcgtatcg agcaggatag aaagggatgc actgacaaca
     6361 gcatcaatta ctttattagc atctgtaaat ctcgaaatga tttctccgac ttttcggttg
     6421 ctgaaaaact tcattggtaa agaaagtaca tgtttgaagt atccgaacat aatggtaata
     6481 cttaatttct gacccagcag gatcatcata tattgcctaa atcctattaa gaaaactttg
     6541 aatagataca taattaaaag gccgattgaa agaatatgta atgtattact tagcccattc
     6601 ggcaagattt gatcaataat cgtttgaaaa taaaaagtac ccagaattcc gaagattgta
     6661 ataataaaag atgctaagaa tgtatgtata atcaaggatt tttgttgaag aattaacctg
     6721 aaaatatctg aacttatatt ccttatctct ttttgttttt cataggcttc ggtgggtgtt
     6781 aaaagtatta agatccctgt ccagatcttt aaaaaatcat cttttttgta tttaataagc
     6841 cctttgccgg ggtctgcaat gattatcttt tctttactga ttttatgtat aacaacataa
     6901 tgcaataatt gttgatctat aacaatatgt gctattgccg gaagagtaat agattgatcg
     6961 aaagctgcat gctctgctcg caccgccttt gcagagaacc ccaattgttc agcggctttt
     7021 accattccaa gaacgctagt tccctgtgta tcagttccgg caatgtcacg gatataagtt
     7081 atagagagct ttgaaccata ttgtcttgca atggttgata aacaagcagc tccacaatct
     7141 ttttcatcca tttgttttac acagtgatat tttttaaaaa acatttttaa tctccatatt
     7201 atagaattgt tgaattatac aaaaattatt atcataatac aataagtatg aatatatcaa
     7261 tagttccggg aggaaatata tttgtttttg aataaaaaat ttttatacat gtatactttt
     7321 tgctaaattt tctatctctt ccttataagt ttccaaatca ttattttcat tatattcaat
     7381 aatcttgtct tgaatagttg aattcattaa aaacaacggt ccgtaattct tttttaattc
     7441 acaaaaccat gcttgttttt ttagactttt taagtttctt ctaaatttca aatttctctt
     7501 tgatggtatg gtagtaacaa taatccataa tatagtctca actagcatgt acaaaaaatc
     7561 catcaaatgt cctccttgag cttaattcac aatgtaaata aaacataatg taagactaac
     7621 ataaaagtac ttttagttaa agtttcaatt aattaatcag agtataagat taaatcttct
     7681 tcagtgagaa tcataaaaaa gagagtggtt attgtaacga atagacgttt tgtaggaatt
     7741 ttagatgaaa aatgtcatgt aattttgctt ttgagtattg aaaaaagtgt aaatcacaat
     7801 tatactaata caagtcgaca ataatttacc aagttcggac ttaggaggag tagtagtatg
     7861 gaaaagcaaa acaatagatt tgagtcttta acagtagaag aagctgaaat gatagatggc
     7921 gggggatacg gttcttattt attaaaagga gtcggaacag gaattgctgt tggagcagcg
     7981 cttggaggac ctggcggggc tgttttaggt gctaatgctg gagctatctg gggttctgca
     8041 gcgtatggtg tagatgcatt aatttctggt cataaataaa aaacataaat tgaacaaaaa
     8101 ttatatatag gaggtttatt atgttacata actttgaaaa aagtaataat atgattgaat
     8161 taagtgaaca agaaatgcta gatactgacg gcgggggctg gagagattta gcaggcgctg
     8221 ctgcaggcgc tgcagtaatg ggaggtacag caatggcttt aggagctgct ggtggcccag
     8281 taggttttgc tgcgggaata gggctggcaa tcttaacaag accagggcat gcaaaataat
     8341 ttgtgttata gaaaagctgt cttatgccga cagcttttct gtattaaaag gaatcttaac
     8401 tgcatacctt tccatgcagt tttaaattga agaacgaaaa ctaaataatt tgtgttaatt
     8461 cctcacaagg tttacttagc caatttaatg gactaattag attttttatg agaatactta
     8521 atcaaagaat ggacacttag aaataataca atgtagaaaa atgagaataa taataactga
     8581 atcagtaatt ctctcacatt ggtaatttct ttagaaagaa aacttatcat tacccaaaat
     8641 aatccgctca atataaaaat aaagaaatgt tttaatcttt ttctttttct ctgtattaat
     8701 aacccccttt ttattgcgaa aattgaataa aaagtgctga caactaaaaa cgatataatt
     8761 atacttagtt gagtgtaaaa taagtttttg gttagaatag aaacaaaaaa actaagaata
     8821 aataaaatta tagacagtcc gaaactgtaa tagctcagtt tatattgttc gtgttgtatt
     8881 ctttcatcta tcattctctc tatcctctct cttaatatca gaaggccaaa aaatatcatc
     8941 taaagtctta ttaaaaacat tacaaatttt tatacataag tttaaactgg ggttatactg
     9001 tcctttttct attaagctca cagtttgtct tgttattcca agtttttcag ccaatgttgc
     9061 ttgagttaaa ccaagtgtta atctttcgac tttaacttta ttattcatgg catcctcctt
     9121 tattaattaa tattgtaaca tatatattcc tttttgcaaa atatatattg cgtagatttg
     9181 gattctaaaa ctgaaataag ctattggaac acttttgcaa gttaagtgac tttcaataaa
     9241 tgactgtgtt ttgagaagaa tttcttgtat tgtacatgag aatggatatt taaggatggt
     9301 gtctttgcat atagacgtta tcatctcttt aagaggattg tttccaaagt ggaaattcat
     9361 tatgacaatg aacaagatag atacatttct cgaaatcagc aaacactaca tactcaacaa
     9421 ctgactacaa ggctgccggt catatcaatc aaatcctgaa atatgttcca tgtccactgc
     9481 ttgagaactg cagaaaatca aagaaccgga agaaaattat attacttagc atgtataggt
     9541 aaaccatagg gaaaaggtca gacaaaatcg cttgtctgtt tcaagaaaaa aactttataa
     9601 aaaaagaaaa taaaaaatag agcgaagctt tgcagatatt ccaaacagct gcatgggctt
     9661 cgctattgca ggttgggtaa aagcggaatg tgagcgagca agttctcctt acatccgcct
     9721 gccggacatg aagaagtttc ggcgccatcc tcatgataag gcttgcggac gtcccgtctg
     9781 atcgcctcga tgatttcttc ttcatgagcg gtgacggcat ctaaaaagcg ctgaagcttt
     9841 tctctgcgct gctcggctgt tgaagcacgc agtgtttttt gatttttttt ctgaagctca
     9901 aagacgcgtt tgatgtctgc ttttatatgt tgatccatcg gtcttcctcc tctggttgct
     9961 tttttgtttc ttcagggcct attatagtgt gatgtgattt tgcggcaaag acagcgtaaa
    10021 cgcatctata cgttaccgta caaattgcgg tgatctgtct aatgaaagga gtgtctctta
    10081 tgccggattc tgtgacaact gacatcaaac aagcgctttt ggccatgctt ggagagcggg
    10141 atatccgccg tgtgaccatg aaggacatcg ccgaacggtc gcatgtgagc cgggggacac
    10201 tgtaccttta ttacgaagat aaatatgcga ttttagaaga tataacggaa gaaatgaaag
    10261 acgggctgag cgaagcgctg tatgattcgc tgaagaataa agatgtgctt catttacatg
    10321 gcggatggca aaaagtgcat ccgaccctgt cttttgttga ccggcatcgg gagttttttc
    10381 aaacgatgat ggaccgtcat aaaatgccgt attttcactt tcatgctttt ttaaaagatg
    10441 tgttccggca tgatgtgctg ctgtcaccgg tcaatgcgga tttctcaccg accgaacagg
    10501 atatgtacat tcattaccgc gcgttataca catatgccat tgtgttgtac tggctgaatg
    10561 aggagcccgg ggcatccccg gaagcgatca gcggacaggt gtgggattta atttctcaaa
    10621 aacggtttta ctggctgttc ggccgccggg tgccggaaca agaagaaaaa aagcccgccg
    10681 accgccgggt gacccggacg aggcaggcct tgcaagaagc catgatcggc ttaatggcgg
    10741 aaaaaaaaga gtacgcggcg attacgattt ccgatattgc ccgtcaaagc aacctccgcc
    10801 gtgccacttt ttatgaccat tacgccaaca aagaagcatt gctgaaaacg atcatccatc
    10861 aatcatgccg ggatctgatc ggattattga cggtaaaagg agagcctgcc gattgctcca
    10921 tggaagaagc ggaagccgcg ctcgtccgtc tcttttccgc actgtcggat ggcctgccgc
    10981 tcgttcattt tctgagagag ggttcgggag tgcctgacgt catcccggaa atgttcagag
    11041 cgctgcaatc gttttatctt catcagcctc tgcacattca ggcggggaaa aagctctatg
    11101 catactatgt cgcgtccatg ctcgccggct tgattcaata tcgtcttcat gaaggaaaag
    11161 accacgcgcc ggacatgctg gccctggagt ttctgcagtt tttggatgtg aagaagtatc
    11221 gggtgatttt gctgtgagga tgatgaaaaa gaactccgaa aacaggcggg gttctttttt
    11281 gctgttttct ttgagaagtg ccgatatgaa gtgtccggct ttatctctta taaaaaagat
    11341 accttccata tgatgcctga tcagcattct gccgccaaac atgacaaatg tcatattcga
    11401 ggcatgatgg ctgctactac aaaagcgtct ggcgccccgt tatacttggg atcataatga
    11461 aaaagaaggg acatgacatt catgatcaat cacgccgcaa ctcaaaaaca agcgtcttta
    11521 aaaaagcagg ttgccgcatt ttccggcgcc gatacaaagc atagtgtcat tcagcttttc
    11581 aacacgtttg tgccgttttt tgccctttgg tttcttgctt acttcagcct cgatgtctcc
    11641 tatcttctga ccctcgcctt tactgttata gcagcgggat ttttgacaag aatctttatt
    11701 attttccacg actgctgcca tcaatccttt ttcaaacaaa agaagctgaa ccggctgttc
    11761 gggtttattt caggtgtgct gacactgttt ccgttccttc aatggcagcg cagccactcc
    11821 atccatcatg cgacgagcag caacttagat aaacggggca ccggcgatat ttggatgatg
    11881 accgtcaagg aatacaacga agcgtcagct tggacaaaag tacgctacag actttatcgc
    11941 aatccgttta ttatgtttat tctcggccca atttacgtat ttctgattaa aaaccgtttc
    12001 aatgtaaaag gcgcccgccg caaggagcgc tggaacacct atttcacgaa cgcggcgatt
    12061 gtcttattgg ctgctgcgac gtgcctgctt gtcggctggg aaaacttttt gcttgttcag
    12121 ggtccgattt tccttatttc ggggtcaatc ggcgtctggc tcttttatgt tcagcacacg
    12181 tttgaggatt cttattttga agccgatgag cattgggatt atgtgcaggc cgcggtagaa
    12241 ggaagctcct tttacaagct tccgaagctt ttgcaatggc tgacgggcaa tatcggctac
    12301 catcatgtcc accacttaag cccgaaagtg ccgaactaca agctggaagc cgctcatgag
    12361 gggcacgagc ctttaaaaaa cgtaccgacc attacgctta aaacaagtct tgaatccatg
    12421 aaattccgtc tgtgggatga ggacgaaaag caatttgtca cctttcggga ggcgcggaaa
    12481 acaccgctgc cccaaccggt accgaaagaa cctttaaaga aaaatgcctg atatggcgcg
    12541 cggctctttc tcttatcaga aagggccttc tcttcatgcg tgaggatgca atgaaggcgg
    12601 tgttttgata aagtgatggt agacacaagc agcagaaggt ggaacaaaga tgaagaagta
    12661 ttttaaattt caaaaactga atgggatttc cccttatata tggacgattt ttttcatctt
    12721 gcctttttat tttattttta aaagctcttc tacctttgtc atcgtggccg gcatcatttt
    12781 cacacttgtt ttctttggcg cataccgctt tgcatttgtt gcaaaaggct ggccgcttta
    12841 cttatgggca cttcttctga tcggcatttc gaccggctca gttatgctgt tcagctatat
    12901 ttatttcgcc ttctttatcg cctacttcat cggccatatc cgagacaaag ttccttttta
    12961 catcttatat tacattcata tcatcagtgc ggcggttgcc gtcaacttca gtcttgttct
    13021 gaaaaaaggg tggtttttga cacaaattcc gtttatcgtc attacactga tcagtgcca
//
