LOCUS       NZ_CP075970            20800 bp    DNA     linear   CON 27-NOV-2024
DEFINITION  Clostridium perfringens strain C269 chromosome.
ACCESSION   NZ_CP075970
VERSION     NZ_CP075970.1
KEYWORDS    RefSeq.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Clostridiaceae; Clostridium.
REFERENCE   1  (bases 1 to 3208157)
  AUTHORS   Jaakkola,K., Lahti,P., Virtanen,K., Lindstrom,M. and Korkeala,H.
  TITLE     Comparative genome analysis and spore heat resistance assay reveal
            niche adaptation within Clostridium perfringens enterotoxin-carrying
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3208157)
  AUTHORS   Korkeala,H. and Jaakkola,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) Department of Food Hygiene and Environmental
            Health, University of Helsinki, Agnes Sjoberginkatu 2, Helsinki
            000066, Finland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020138715.1-RS_2024_11_27
            Annotation Date                   :: 11/27/2024 04:03:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,876
            CDSs (with protein)               :: 2,876
            Genes (RNA)                       :: 128
            rRNAs                             :: 10, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 22 of 38
            Pseudo Genes (internal stop)      :: 10 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:22:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1981745
            Orig. end                         :: 2002545
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075970.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20800
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="2"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20800
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20800
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10800](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10800)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     misc_feature    complement(495..511)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 25.64"
                     /tool="antismash"
     gene            632..1168
                     /gene="lepB"
                     /locus_tag="KLF29_RS08600"
                     /old_locus_tag="KLF29_08550"
     CDS             632..1168
                     /EC_number="3.4.21.89"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /GO_process="GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /gene="lepB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464478.1"
                     /locus_tag="KLF29_RS08600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08550"
                     /product="signal peptidase I"
                     /protein_id="WP_195402786.1"
                     /transl_table=11
                     /translation="MKCFNYILDLPDSFFKIIIAILISLILILGFFLNNIFIAVVDGSS
                     MEDTIHHGDVLIINKKSYSTTSPKRYDIVNIYAPCKYDNFLVKRIIGLPGDTIEINNSE
                     VYINGDKIYESYIKEEMNLPYYLKLKIPKDKFFVMGDNRNISLDSRYFGLVQDTDIQGK
                     AIFKYCYKNHKFKLF"
     gene            complement(1225..2085)
                     /locus_tag="KLF29_RS08605"
                     /old_locus_tag="KLF29_08555"
     CDS             complement(1225..2085)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449700.1"
                     /locus_tag="KLF29_RS08605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08555"
                     /product="hypothetical protein"
                     /protein_id="WP_195402787.1"
                     /transl_table=11
                     /translation="MIKKIVVAVGLAFSLSFLVGCGNKSLVEQGKAAIKQGNYEEATAI
                     FKAASEEDSKDKEEAESLYDLTYNYVISNKAYNEFKLDVALDSLKKVEENKNKELMKED
                     ITSLKEEISKDKSVLDDFYSSMEKVNDLESEGKISEARDLLNKSLKNISYLGENVKELI
                     ASGNSKLKNLDEKLDKKEIVKEDINKEDNNKEEKTDSSEKNNKELANKALETVKNLGIV
                     KKDQYLELKSDEVYDLGDGSYGFVVNKYYYPEGKPEPAFICQYLVDKESWKVKEIKEGI
                     KKELN"
     gene            complement(2194..2463)
                     /locus_tag="KLF29_RS08610"
                     /old_locus_tag="KLF29_08560"
     CDS             complement(2194..2463)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590892.1"
                     /locus_tag="KLF29_RS08610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08560"
                     /product="ATP cone domain-containing protein"
                     /protein_id="WP_003449692.1"
                     /transl_table=11
                     /translation="MNIIKKDGRIEEFKIEKLSTSLSNAASDLNFTLNKSDINIIAKDV
                     EETLKSIRKDNGETSSYEVIGVVIDTLKNEKFSDVLRAYVKYRK"
     gene            complement(2658..4673)
                     /locus_tag="KLF29_RS08615"
                     /old_locus_tag="KLF29_08565"
     CDS             complement(2658..4673)
                     /EC_number="2.7.8.-"
                     /GO_function="GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449764.1"
                     /locus_tag="KLF29_RS08615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08565"
                     /product="LTA synthase family protein"
                     /protein_id="WP_224379946.1"
                     /transl_table=11
                     /translation="MNKKYFKEVLGNKKTLKNLGINVFIWITLSFLLVFLGESFHRGSI
                     ENGKDFLDNQTTVFLLNYLIFLMITSIAFFFKKTRMVYGIISTILMGFYMASGVILGFR
                     GTPLIWADMFSFKEGLAIAGNYLNLNILKYAVIALVIIIAILVLLWFSERYKSRNKVIN
                     LYGFIILPLSILAVGAFYGNAKGSIEVYRWDLPVSYERNGFMYSFLDTAAGFKVKEPSD
                     YNKASIEKIKNDIIEEAQLASNDTKMASVMPAKFPNIIIVQLESFMDLDRINGLTFTED
                     PIPTFRKIASESTNGFLKVPTYGGGTVRSEFEVLTGLSTDYLPVGEIPNNNILKKQPVE
                     SLAYILRDYGYGTNVIHNYEGNFYNRDTVYPNLGFDKYISMEYMDKPTNADWQYPEDVL
                     NIEPIEDIISNNEKPQFIYNVTVESHGGYSSSDFENYTVDGDLDQEEKNELQCYIDKLR
                     GVDEYIKELLDYVESSGEPTVIAMFGDHLPSLKIINDDESVLKDGNKYLADFFIWDNIG
                     LPKENVNMEAEEFTTYILEKLNMVAGVMPTFHNACKDDENYKEDFELLQYDMLFGNKYI
                     LNENKNKYEKTNMKMGLKEITLNNYDIKDDILTVTGDNFNYKSKIIINGKIKETNFIDE
                     NTLTTTEIPSNIKNISVGQIGKYDKILSSSNSLEIK"
     gene            complement(4975..5925)
                     /locus_tag="KLF29_RS08620"
                     /old_locus_tag="KLF29_08570"
     CDS             complement(4975..5925)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481670.1"
                     /locus_tag="KLF29_RS08620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08570"
                     /product="aromatic acid exporter family protein"
                     /protein_id="WP_003449684.1"
                     /transl_table=11
                     /translation="MRFIGVRTWKTGIGAVIAMIIAKELGLSYWVSAGIITILSIQSTK
                     RESLKIAIKRIESVIIALIVSSVLFLALGFNSVVFGLYLLVFIPLTVKLKVTDGIVVSS
                     VLVTHLLVEKQVNSYWIINELGLMLVGVLVALILNSYMPKNEEKIKEDIDYISEKIKEI
                     FMDMAYSLRTHSVSIKEQRLFDELENRIELAKKRANDNFNNYLFSDVKYYVHYIDMREV
                     QFQMLKYMREHFSRISITVKQTELVANFTEEVASVIGKEVNVNILINRLNRLKKELSEQ
                     ELPVTREEFENRAMLFQFINDLEIFIKTKNQCYIK"
     gene            6073..6714
                     /locus_tag="KLF29_RS08625"
                     /old_locus_tag="KLF29_08575"
     CDS             6073..6714
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468041.1"
                     /locus_tag="KLF29_RS08625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08575"
                     /product="undecaprenyl diphosphate synthase family protein"
                     /protein_id="WP_042268110.1"
                     /transl_table=11
                     /translation="MRIPKHIGVIPDGNRRWAVENGLEKDQGYLHGIEPGLELFKLCQK
                     LDIKEITYYGFTTDNTKRPSYQKKAFIDACIKSVELIANEDCELLVIGNADSPVFPEEL
                     KKYTKRTKMGKGGIRVNFLVNYGWEWDLNLLKACDIKSKNIYDNIQSKDISRIDLLIRW
                     GGRRRLSGFLPVQSVYSDIYIIDDLWPNFNKDHFFNALEWYKDQDITLGG"
     misc_feature    6082..6096
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.02"
                     /tool="antismash"
     gene            6719..8218
                     /locus_tag="KLF29_RS08630"
                     /old_locus_tag="KLF29_08580"
     CDS             6719..8218
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476382.1"
                     /locus_tag="KLF29_RS08630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08580"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_224379947.1"
                     /transl_table=11
                     /translation="MSSKKKAIIVGAGIGGLATAVRLLVNNFEVDIFEKNSKIGGKVNL
                     IEYKDFKIDSSASIFMLPKPYLEVFKYAKKDPKDYIELVELNTLYKVFNDEGDSFNIYS
                     DFLKTTESLEKVFNDESSNYYKYISDSYRRYLLVEKYFLNRSFFTLNYWRYFKSLPELI
                     KIHPFKNCYKTIEEYISNEYLKTLLAFQCMYIGESPLKSSNVFNLIPSTTQIYGLYYIK
                     GGMYSYVKALEKLILELGGKIHLNSNVTNIIMEKNVAIGAKINHENIFSDLIVCNSDFT
                     YTIQNLLPRSTFKNKISRRKQNNLSFSCSTFILHLFLKKKYKNLHVHNIVLNLNKKEVL
                     LAPFIDGPLPKEYIYYIYCPSSIDTSLTPPDCECINITVRVPNLKKYKSKWNEFTIVSL
                     KNKILYDLSKIKGLEDIKENIIYESYTTPLTLKNDFNCFFGAAFGLNHNLLQTTIFRPQ
                     AKIKKLKNIYFVGDSVHPGSGISMSLISAKLCCEKIISDFS"
     gene            complement(8250..9941)
                     /locus_tag="KLF29_RS08635"
                     /old_locus_tag="KLF29_08585"
     CDS             complement(8250..9941)
                     /EC_number="2.7.7.65"
                     /GO_function="GO:0009975 - cyclase activity [Evidence IEA]"
                     /GO_process="GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025: diguanylate cyclase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010508.1"
                     /locus_tag="KLF29_RS08635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08585"
                     /product="diguanylate cyclase"
                     /protein_id="WP_142420948.1"
                     /transl_table=11
                     /translation="MIRDVNDNKKKKVDEILSIVKVSSLLFSAMAFLQYTFNLTKYNNK
                     FLAYYSILSILISIFLLSTIYILWSLASLNKYKDHINKIQLIEIILFMIIFIMAILVSG
                     GNSSNNKFLFLFIIITSTIQGGMHKGVLISVVSSAIILAIDLIFSGNADVNIYFQNDLI
                     LAGVFVLTAWTLGFYVKTENDHIKELQTLVNEDGLTGVYNHRYFYDKLREKMHISEVEN
                     EKLSLIFMDIDYFKHYNDLYGHQKGDEALKKIGKLLKNIARENDLVARYGGEEFAIILP
                     NTSEEEATKIAERIRNKVEKTYFEGQENQPNANFTVSVGIAVFPDKAKSELDLVKCADD
                     ALYRAKFFHKNRVETYVSVLDELKRDLNEEENALVASIKTLISIINAKDKYTYSHVERV
                     VIYSRLMAKKLGLSEDEKKQLIYGAYMHDIGKINIPNEILTKKMRLTKEEWEILKQHPK
                     DGVEIIKSVNSLKNVVPLILYHHERYDGFGYPVGLKEKEIPYLARVLTVIDSFDAMTSS
                     RPYNKRKTYDEGLIELERCSGTQFDPEIVGIFSEVVKENINEIQDF"
     gene            complement(10001..10135)
                     /locus_tag="KLF29_RS08640"
                     /old_locus_tag="KLF29_08590"
     CDS             complement(10001..10135)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: TIGR04223"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468055.1"
                     /locus_tag="KLF29_RS08640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08590"
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="WP_003449588.1"
                     /sec_met_domain="TIGR04223 (E-value: 4.3e-15, bitscore:
                     52.8, seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLNKNLLTLFAALTTVVATTVATSACLWFTHQPEEPKSLRDE"
     gene            complement(10156..10800)
                     /locus_tag="KLF29_RS08645"
                     /old_locus_tag="KLF29_08595"
     CDS             complement(10156..10800)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592481.1"
                     /locus_tag="KLF29_RS08645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08595"
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_003455887.1"
                     /sec_met_domain="AgrB (E-value: 2.7e-44, bitscore: 148.1,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIENISKLIAEKVSSELNYDNERKEIIQYGTYALIQTLISIISVL
                     ILGLVFNIALEALIFLFTASILRKYSGGAHSESSNVCTLLGIIISICIGFLIKSSFFAK
                     MNFELVVFIGIVIFVFGYFIVFKFAPVDTKNKPIKTEKKKKRMKKGSLKILTIYLFIEV
                     LSIILYYNSGWSLAKPVMLSIIFGVAWQCMTLTYIGNILLKTIDSFTNKLL"
     gene            complement(10802..11263)
                     /locus_tag="KLF29_RS08650"
                     /old_locus_tag="KLF29_08600"
     CDS             complement(10802..11263)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455966.1"
                     /locus_tag="KLF29_RS08650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08600"
                     /product="hypothetical protein"
                     /protein_id="WP_003449794.1"
                     /transl_table=11
                     /translation="MLKVTLFETLVRGIPEALTMMLAMYAFANKKLEKKEYLISSLILV
                     LVVYLIRLLPINYGIHTILNIFVLIFLAFNVNKIDLIVSIKASILILMILFLCEGLNLL
                     FIEKFFNENIDYLFENVFTKTILGIPSTIMFMVIVTYYYLIASKKGKLR"
     gene            complement(11280..11846)
                     /locus_tag="KLF29_RS08655"
                     /old_locus_tag="KLF29_08605"
     CDS             complement(11280..11846)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456112.1"
                     /locus_tag="KLF29_RS08655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08605"
                     /product="DUF5317 domain-containing protein"
                     /protein_id="WP_003449620.1"
                     /transl_table=11
                     /translation="MIEAVIIALIISKIKGYSLKPFFKSWTIYPILIFEAIYIVFQISI
                     FMGNYDVVKWAPWFKSIYMYLFLIPIFWYKEYVSALIGSLFILAGTFLNHIVMAANGGE
                     MPVFPSLSYLTGYLKPNTISKVNDIHMIGTSTVKYKYLSDIIDVGYSVLSIGDILIRVF
                     VVIIIYVTVKKTNEKNEYKQRNIFA"
     misc_feature    11949..11962
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.36"
                     /tool="antismash"
     gene            complement(11998..12501)
                     /locus_tag="KLF29_RS08660"
                     /old_locus_tag="KLF29_08610"
     CDS             complement(11998..12501)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468057.1"
                     /locus_tag="KLF29_RS08660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08610"
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="WP_003464512.1"
                     /transl_table=11
                     /translation="MFISVIIALLGIVPSVFVTGANIVFFGPINGFLISLLGEVIGGWI
                     SFKVYRKGINKFAGNIEGKYELIDKIVKSEGRNVGILIFEGRLIPFIPSGLVTLAAAMS
                     KVNSFTFIISTFLGKIPSILLEVLASYGVIMASQKNLKLVIGVLSLILFLLTLKKLKDK
                     TNKK"
     misc_feature    12484..12499
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 25.19"
                     /tool="antismash"
     misc_feature    complement(12589..12604)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 23.7"
                     /tool="antismash"
     gene            complement(12818..13813)
                     /locus_tag="KLF29_RS08665"
                     /old_locus_tag="KLF29_08615"
     CDS             complement(12818..13813)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590894.1"
                     /locus_tag="KLF29_RS08665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08615"
                     /product="calcium/sodium antiporter"
                     /protein_id="WP_011010510.1"
                     /transl_table=11
                     /translation="MNYIFLIIGFLLLIKGADLFVDGASGIAKKLGVPAVIIGLTIVSL
                     GTSAPELAVSISASLKGSNDITMGNVLGSNLFNLLAALGCTAIVAPLVIKKHIIKNDFI
                     VNLIATFILYVFTFTGLIGSKNSLSRIDGAVLVLMCITYIIYLIYTVKRSNKREGEVAV
                     TAEFEDFEEQNIKSPNSTVKNIILSIVGVIGIILGGKIVVDSASAIALGLGLSEKLVGL
                     TIVAIGTSLPELVTSLVAAKKGENDIALGNILGSNTFNILLILGLSSVISPITIAASLS
                     IDLIFLIIVTLIIGALIFLNKKKEKVLTRYEGVFLLALYIGYTVYIIMRN"
     gene            complement(13943..15022)
                     /gene="dinB"
                     /locus_tag="KLF29_RS08670"
                     /old_locus_tag="KLF29_08620"
     CDS             complement(13943..15022)
                     /EC_number="2.7.7.7"
                     /GO_function="GO:0003684 - damaged DNA binding [Evidence
                     IEA]"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /gene="dinB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455932.1"
                     /locus_tag="KLF29_RS08670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08620"
                     /product="DNA polymerase IV"
                     /protein_id="WP_224379948.1"
                     /transl_table=11
                     /translation="MKENRKIIHIDMDAFYASIEQRDNPKYKGKPLIVGGDPNRRGVVA
                     TCSYEARKYGIHSAMPSLTAYKLCPKAIFIRPRMEVYKKVSRQVMNILNEYSNLVEPLS
                     LDEAFVDVSKSKRCKGSATLMALEIKERIFKEVGLTASAGVSFNKFLAKMASDFRKPDG
                     ITVITEENSKDFIRNLPIGKFFGVGRVTKNKLNNIGIFKGEDLLKFSEEELIDIFSERG
                     KILYEFARGIDNRPVNPYRIRKSIGKEITLREDIEDIEEMIEILEEIAERVSESLDLLN
                     KKGKTVTLKVKFNDFKHITRSITLEHFLKEKKEIMECVKDLISIVDFKNKKVRLLGITI
                     SSLEENIITEEREQLSFDV"
     misc_feature    15035..15048
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.07"
                     /tool="antismash"
     gene            complement(15165..15797)
                     /locus_tag="KLF29_RS08675"
                     /old_locus_tag="KLF29_08625"
     CDS             complement(15165..15797)
                     /EC_number="5.2.1.8"
                     /GO_function="GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /GO_process="GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464494.1"
                     /locus_tag="KLF29_RS08675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08625"
                     /product="peptidylprolyl isomerase"
                     /protein_id="WP_070956958.1"
                     /transl_table=11
                     /translation="MSLLKKVSKIVVGVTMASLLLIGCGGTGKKAKENIPEDKLPVATI
                     EVKNFGTIEAELYPNKAPNTVDNFISLANSGFYDGLTIHRIVKGFVLQGGDPKGTGTGG
                     PGYMIKGEFSSNGVENDIKHEEGILSMARSQNPDSAGSQFFIVTKEAPHLDGQYAAFGK
                     VTKGLDVVHEIEKVSVGANDKPVEDVVIESIKVDTKGIEYNEPEKVK"
     gene            complement(15975..16379)
                     /locus_tag="KLF29_RS08680"
                     /old_locus_tag="KLF29_08630"
     CDS             complement(15975..16379)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449702.1"
                     /locus_tag="KLF29_RS08680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08630"
                     /product="DUF3785 family protein"
                     /protein_id="WP_003468091.1"
                     /transl_table=11
                     /translation="MFSFTFDEKEYTLSEDKLLYFFNEDVEGFDIDKLFEILNSSEGIS
                     FGKAYYDGPCEECKFGLEEKKKSFPFLEFNMFIYAKNGKFVISNIEKAYEGLTYNKLLR
                     DKKVDKSYLVNINVCKNCGNFAVEVEELEV"
     gene            complement(16496..17326)
                     /locus_tag="KLF29_RS08685"
                     /old_locus_tag="KLF29_08635"
     CDS             complement(16496..17326)
                     /EC_number="3.1.3.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1179: hydrolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455945.1"
                     /locus_tag="KLF29_RS08685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08635"
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="WP_003455945.1"
                     /sec_met_domain="HAD_2 (E-value: 5.5e-06, bitscore: 24.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYKLVCIDMDGTLLDSHHKVSERNKEALKKAIEKGVHIAISTGR
                     VFPSARIYGNIIGVNAPLICSNGSYIKDKNTDEVIFKSTLDRETYFKICDIINKYKFLA
                     YVDSTDGLIADTDIPEDDSHRLMNSWVDEEEKIKFYKYDDLRDAYDEHGEDILKFIIIK
                     QEGGPNIEEAKEEFEALEGVDLVYASWGGCIEIMKKGTSKGSAVKALADKLGISLEEVI
                     CIGDSGNDVSMLEEAGLSVVMGNAPDFIKEYGDYITDTNENDGVAKAIEKFILE"
     gene            complement(17630..18343)
                     /locus_tag="KLF29_RS08690"
                     /old_locus_tag="KLF29_08640"
     CDS             complement(17630..18343)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010514.1"
                     /locus_tag="KLF29_RS08690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08640"
                     /product="PspA/IM30 family protein"
                     /protein_id="WP_003455961.1"
                     /transl_table=11
                     /translation="MGIFNRISNMIKAKTNSALDEMENPVELLDQKIRDMEKSFNEAKR
                     SSAQIFGNLKDTEKKMNEAKEESAQYDEKVRLAMSKGNEELAKKALKLKLDSDKKFESL
                     KASYEGQRAKADVLKAKLVELEKELDKTRSYRDEAVARLNNAEASKQINEVIANVQSKS
                     NSINIDDIERSISRKESYAAGLEELKGESLDDEFAKLEELSLDDELNKYRTQNTQEDKT
                     SDSEVDLELEKYKNK"
     gene            complement(18429..19130)
                     /locus_tag="KLF29_RS08695"
                     /old_locus_tag="KLF29_08645"
     CDS             complement(18429..19130)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449564.1"
                     /locus_tag="KLF29_RS08695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08645"
                     /product="hypothetical protein"
                     /protein_id="WP_003455926.1"
                     /transl_table=11
                     /translation="MKKHKRFGQLVLFFIAFFFLVGCFNISPIKKVKAETFGIENTIAK
                     GGRSSGGGSRSSSRSSGSSSRSSSKSSSYNSSKKYSKPSSSSSEKKKSQGNSNYSTTDK
                     FNKPSSGSNSSSDKVESGKDSSNSDKGSTQGNNNYSTTDKFNSKAPNNQEGSSKENNKY
                     ENNDKSSGSSNKSYRSSNSGGFSFFDMLMLNNILNMFGNMFSFMFIRRPFIGIGTILLI
                     ALIIFAIRKRR"
     gene            19278..19943
                     /locus_tag="KLF29_RS08700"
                     /old_locus_tag="KLF29_08650"
     CDS             19278..19943
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475402.1"
                     /locus_tag="KLF29_RS08700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_08650"
                     /product="hypothetical protein"
                     /protein_id="WP_224379949.1"
                     /transl_table=11
                     /translation="MNAKEFLDKEKNTYGNLYSELIFVLDDIEDLSEDSTLKERAYYKN
                     IKILKKYNDLLDKLSRELPEKKSFLSFLNKSKENEIIKECEDFKKTYKDSLDKIFVCSK
                     CMCVKCIRECGFNPCLSCNKTGKVNYCDKENTNLIVFKNFIKQQYNNETNENEPIEILC
                     EVEMLDMDKSFRIIKEVLSGEQFVLEYVYNIKDGDLYNSIEDVDLFNEIIDIYESNKI"
     misc_feature    complement(20647..20664)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 25.17"
                     /tool="antismash"
ORIGIN
        1 ctgaactctt atcaattgaa aaatctctta tatgcatctc atataaaatt gaatctaatg
       61 gattttcaag tgatggtttc ttttcatttt cccatccctt aggattagtt ttcttcaaat
      121 caataaccat agctctatac ccattaactc ctaaggcttt gcagtaagga tcaacaaaat
      181 catgctcaaa atcttctcct acaatatggt acttataata aactccattt aaatctcctt
      241 tctttactaa ttcccaaagt ccattacttt ttttatgaag caaatctctt tctattaaac
      301 tctctgtatt cccatctcta taaagtaata attctaaact cttagcttct ggtgaccata
      361 ccctaaaaat agtctcctct ttattataaa tagctccaag ttctccatca taataatact
      421 tatcattaaa ctctttagaa ttaaaatttc ttctcataaa atcctctccc catttcggta
      481 acgtttccaa ttaactgtat ataaattaca actaacttaa ttatattaaa gtagtaatta
      541 aatttcaact taaagttaat tattagttct tatttgaata cataaaaaat aatctaataa
      601 tatattctta atatacatga ggtaataatt aatgaaatgc tttaattata ttttagatct
      661 acctgatagc ttttttaaga ttataatagc catattaata tccttaattc taattttagg
      721 tttcttctta aacaatatat tcatagctgt tgttgatggc tcttcaatgg aagatactat
      781 tcatcacggt gatgtattaa ttataaataa aaaatcctat tccaccactt ctcctaagag
      841 gtatgatata gtaaatattt atgcaccttg taaatatgat aattttctag ttaaaagaat
      901 cataggtctt cctggtgata caattgaaat aaataattct gaagtatata taaatggtga
      961 taaaatttat gaatcatata taaaagaaga aatgaatctt ccttattatt taaaattaaa
     1021 gatacctaaa gataaattct ttgttatggg tgataatagg aatataagtt tggattctag
     1081 gtatttcgga ctagtacagg atactgatat tcaaggcaag gcaatattta aatattgcta
     1141 taagaatcat aaatttaaat tattttaatt taaattaaac aaactagaga gaataaaaat
     1201 atcactctag tttgtcataa atttttaatt taattctttt ttaattccct ctttaatctc
     1261 tttaactttc catgattctt tatctactaa atactgacat ataaatgcag gctctggctt
     1321 tccttctgga taatagtatt tatttactac aaacccgtaa cttccatcac ctaagtcata
     1381 tacttcatca gattttaact ctaaatactg atcctttttt actataccaa gattctttac
     1441 tgtttctaaa gctttatttg ctaattcttt attatttttt tcggaggaat cagttttttc
     1501 ttctttatta ttatcttctt tattgatatc ttctttaact atttcctttt tatcaagctt
     1561 ttcatctaaa ttctttaatt tagaatttcc acttgcaatt agttccttaa cattttctcc
     1621 taaataagaa atatttttaa gagatttatt taacaaatcc cttgcttctg atatcttacc
     1681 ttcactctct aaatcattaa ccttttccat acttgagtaa aagtcatcta gtacactctt
     1741 atctttactt atttcttctt ttaaactagt aatatcttct ttcattaatt ctttattttt
     1801 attttcttct actttcttta aactatctaa agccacatct aacttaaatt cattatatgc
     1861 tttattagat attacataat tatatgttaa atcatataag ctttcagctt cttctttatc
     1921 ctttgaatcc tcttcactgg ccgctttaaa aattgctgta gcttcttcat aatttccttg
     1981 ttttatggca gctttacctt gttctactaa acttttattt ccacacccaa ccaaaaaact
     2041 taaactaaat gctaaaccta cagctacaac tatttttttt atcatatttt cacttctctc
     2101 ccctatttac cattaataaa atatactctt tacaaataaa gggcatgtat caaaataagt
     2161 tttatttgtt atatttgata cagccccttt taattatttt ctatatttta catatgctct
     2221 taaaacatca ctaaacttct cattttttaa tgtatcaatt acaaccccaa taacttcata
     2281 acttgaagtt tctccattat cttttcttat tgattttagt gtttcctcaa catcttttgc
     2341 aattatattt atatctgatt tattaagtgt aaaatttaaa tcacttgctg cattagataa
     2401 gcttgttgat aatttttcaa ttttaaattc ttcaattctt ccatcttttt ttattatgtt
     2461 cataaatatc ctcctgttaa attagtataa tttagtaatt cttaaattaa gtataacatt
     2521 tacattttat tcttaaaaga taaatttatg ctaagagcta ataaataaaa ggctattctt
     2581 aaatttttaa aattatatgt tatgaattta attaaaatca tataattttt gaatagcctt
     2641 ttatataaac taaactacta ttttatttct aatgaattgc tactacttag tattttatca
     2701 tactttccta tttgaccaac tgaaatattt ttaatgtttg aaggaatttc agtagttgtt
     2761 aatgtatttt catctataaa attagtttct ttaatctttc catttataat aattttgctc
     2821 ttataattaa aattatcacc agttacagtt aatatatcat cttttatatc ataattattt
     2881 agagttattt cctttaatcc catcttcata ttggtttttt catatttatt tttattttca
     2941 tttaggatat atttatttcc aaatagcatg tcatattgaa gaagttcaaa atcttcttta
     3001 taattctcat catccttaca tgcattatgg aaagtaggca taactcctgc caccatatta
     3061 agcttttcta atatatatgt tgtaaactcc tctgcttcca tatttacatt ttcctttggt
     3121 aagccaatgt tatcccatat aaagaagtca gctaaatatt tatttccatc ttttaataca
     3181 ctctcatcat catttattat ctttaatgaa ggtagatgat ctccaaacat agcgattact
     3241 gtaggctctc ctgaagattc tacataatct aaaagttctt ttatgtattc atcaacccct
     3301 cttaatttat ctatgtaaca ttgaagttca tttttttctt cttgatctaa atctccatcc
     3361 actgtataat tttcaaaatc tgaagaagaa tatcctccat gactttctac agttacatta
     3421 taaataaact gaggcttttc attattagat attatatctt ctataggttc tatgtttaaa
     3481 acatcctctg gatattgcca atcagcatta gtaggtttgt ccatatactc catagatata
     3541 tatttatcaa atcctaagtt aggatacact gtgtctctat tataaaaatt tccttcgtaa
     3601 ttatgtataa catttgttcc atacccataa tcacgtaata tataagccaa actttcaact
     3661 ggctgttttt taagtatatt attattaggt atttcaccta ctggtaagta gtcagtactt
     3721 aatccagtta gcacttcaaa ttcacttcta acagttcccc ctccataagt aggcaccttt
     3781 aaaaagccat ttgttgattc actagctatt tttctaaagg tcggtatagg gtcctctgta
     3841 aaagttaatc catttattct atctaaatcc ataaatgatt ctaattgaac aattattata
     3901 ttaggaaatt ttgctggcat aacagatgcc attttagtat cattactagc tagttgtgct
     3961 tcttcaatta tatcattctt tattttctca atagatgcct tattgtaatc acttggctct
     4021 ttaaccttaa atcctgctgc agtatctaaa aatgagtaca taaacccatt tctctcatag
     4081 gaaactggca aatcccatct atatacttct atactccctt ttgcatttcc ataaaaagct
     4141 cctacagcca aaatagataa tggtaagatt ataaagccat atagattaat cactttattt
     4201 ctacttttat atctttcact aaaccataac aatactaata ttgcaattat aattactaag
     4261 gctataacag catattttaa aatatttaag tttaaataat ttcctgctat agctaatcct
     4321 tctttaaaag aaaacatatc tgcccatata agtggtgttc ctctaaagcc tagtattact
     4381 ccacttgcca tataaaagcc cataagaata gtgcttatta ttccataaac cattctagtc
     4441 ttcttaaaga aaaaagctat tgaagtaatc atcaaaaata ttagatagtt taatagaaat
     4501 actgttgttt gattatctaa aaaatccttt ccattttcaa tagatcctct atgaaatgac
     4561 tcccctaaaa atactaatag aaaagataga gtaatccaaa taaatacgtt aatccccaaa
     4621 ttcttcaaag tttttttatt tcctaaaacc tctttaaagt actttttgtt cattttatgc
     4681 ctcccttaga acgaatgtaa caaaacaaga cttaattaag aattaaattt ataaatctat
     4741 attgattatt ctaacttatt taagaatttt atgctatttt aattttgtta ataatcgtta
     4801 aatgaatatt aatcatattt actaatataa tatattttgc atataatttt atttattgta
     4861 ttttatttca tcataattat agccaaactt tatattttta accttattaa tagtaaaaca
     4921 tgtaagttta tctaaatatt tttatcttaa tatttatatt taaattataa ttttttattt
     4981 tatataacat tgattttttg tttttataaa gatttctaaa tcattaataa attgaaataa
     5041 catagctcta ttttcaaact cttctctggt tactggaagt tcttgttcac ttaactcttt
     5101 ttttaatcta tttaatctat taataagaat atttacatta acttctttac ctattacaga
     5161 ggctacctct tctgtaaaat tagcaactag ttcagtttgt tttacagtaa tagatattct
     5221 tgaaaagtgt tctctcatgt actttaacat ttgaaattga acttctctca tatctatgta
     5281 atgcacatag tacttaacat ctgaaaacaa atagttatta aaattatcat tggctctttt
     5341 tttagctaac tctattctat tttcaagttc atcaaaaagt ctttgttcct ttattgaaac
     5401 actatgtgtt cttaaagaat atgccatatc cataaatatc tcttttattt tttcagatat
     5461 ataatctata tcttctttta tcttttcctc attcttaggc atataactat ttaatattaa
     5521 tgcaactaaa accccaacta gcataaggcc tagttcattt attatccaat atgaattcac
     5581 ctgtttctca actaataaat gagttactaa aacagaactt acaacaattc catctgtcac
     5641 ttttagtttt actgttaaag gtataaaaac taaaagatat aatccaaaaa caactgagtt
     5701 aaatcctaat gctaagaata aaactgaaga cactattaag gctataataa cagattctat
     5761 tctctttata gctatcttta atgattctct ttttgtactc tgtatgctaa gtatggttat
     5821 aattcctgct gaaacccagt aacttagccc taattccttt gctattatca tagctattac
     5881 agcgcctatt cctgttttcc aagttctaac acctataaat ctcatttttt caactccgta
     5941 taattaacat taaatttttc catagaataa taatataatt aaaatttaaa ataatcaaat
     6001 ttatgcattt tatttattaa aatgggaata ctttcatttg aagctatttt taagaaaggg
     6061 ttgagacata ttatgagaat tccaaaacat ataggggtta tcccagatgg caatagacgt
     6121 tgggctgtag aaaatggact tgaaaaagat caaggatatt tacatggaat tgaacctgga
     6181 cttgaacttt ttaaactttg tcaaaaacta gatattaaag aaataactta ttatggtttt
     6241 actacagaca atacgaaaag accatcatat caaaaaaagg cattcataga tgcctgcata
     6301 aaatcagtag agttaattgc aaatgaagat tgtgaattgc ttgttatagg gaatgctgat
     6361 tctccagttt ttcctgaaga gttaaaaaaa tatactaaaa ggactaaaat gggtaaaggt
     6421 ggcatacgcg ttaacttcct tgttaactat ggttgggaat gggatttaaa ccttctaaaa
     6481 gcctgtgaca ttaagagtaa aaatatctat gataatattc aatcaaagga tatttcaaga
     6541 atagatttat taataagatg gggcggtaga cgtagattaa gcggattttt gccagttcaa
     6601 tcagtttatt ctgatatata cataattgat gatttatggc ctaattttaa taaagatcat
     6661 ttctttaatg ctcttgaatg gtataaagat caagatatta ccctaggcgg ttaattaaat
     6721 gagttcaaag aaaaaagcta taatagttgg agctggaata ggtggattag ctactgctgt
     6781 tcgccttctt gttaataatt ttgaagtaga tatttttgaa aaaaactcta agataggtgg
     6841 aaaagtaaat ttaattgaat acaaagattt taagatagat tcttctgctt caatatttat
     6901 gctccctaag ccctatttag aagtatttaa atatgcaaaa aaagacccta aagactatat
     6961 agaacttgta gaattaaata ctttatataa agtgtttaat gatgaagggg atagttttaa
     7021 tatttattca gactttctaa aaactacaga gtccttagaa aaggtattta atgatgaaag
     7081 ttcaaattat tataaatata tatctgactc ctatagaaga tatcttttag tagaaaaata
     7141 ctttttaaac aggagttttt tcaccttaaa ttattggaga tattttaaat ctttacctga
     7201 actaattaaa atacatcctt ttaaaaattg ttataaaacc attgaagaat atataagtaa
     7261 tgaatattta aaaaccttat tagcttttca atgcatgtat attggggagt ctccccttaa
     7321 aagttctaat gtttttaatt taattccatc aaccactcaa atatatggat tatattatat
     7381 taaaggtgga atgtactctt atgtgaaggc tttagaaaaa ttaatcctag aacttggcgg
     7441 aaaaatacat cttaactcaa atgtaactaa tataattatg gaaaagaatg tagcaattgg
     7501 agcaaaaata aaccacgaaa atatattttc tgatttaatt gtttgtaatt cagattttac
     7561 ttataccata caaaatttac ttcctagaag tacatttaaa aataaaattt ctaggcgaaa
     7621 acaaaataat ttatcctttt cttgctctac atttatacta catttatttc ttaagaaaaa
     7681 atataaaaat ttacatgtac ataatatagt acttaattta aataagaaag aagttttatt
     7741 agctcccttt atagatgggc ccttgccaaa ggaatatata tattatatct attgcccaag
     7801 ctcaatagat acttcattaa ctcctccgga ttgtgaatgc attaatataa cagtacgtgt
     7861 tccaaactta aaaaaatata agtcaaaatg gaatgaattt acaattgttt ctttgaaaaa
     7921 taaaatctta tatgacctaa gtaaaattaa aggtttagaa gatataaaag aaaatataat
     7981 ttacgaaagc tatacaacac ctctgacctt aaaaaatgat tttaattgct tttttggtgc
     8041 agcttttggt cttaatcata atttattaca aacaactatt tttagacctc aggcaaaaat
     8101 aaaaaaacta aagaatatat attttgtagg tgattcagta catcctggct ctggaatatc
     8161 aatgtcttta atctcagcta agctatgctg tgaaaaaata atatcagatt ttagctaaaa
     8221 taaaaagagg tgtattatga tacatctctt taaaaatctt gtatttcatt tatattttcc
     8281 ttaaccactt cactaaaaat accaactatt tctggatcaa attgagttcc actacatctt
     8341 tctagttcta ttaatccttc atcataagtt tttcttttat tataaggcct acttgaagtc
     8401 atagcatcaa aactatctat tacagttaag acccttgcta agtatggtat ttctttctcc
     8461 tttaatccta ctggatatcc aaagccatca tatctctcat gatgatataa aataagaggc
     8521 acaacattct ttaaggaatt tactgatttt attatttcta ctccatcttt aggatgttgc
     8581 tttaaaatct cccattcctc tttagtaagt ctcatctttt tagttaatat ttcattaggt
     8641 atatttattt tacctatatc atgcatataa gctccatata ttaactgctt tttctcatct
     8701 tctgacaatc ctaatttttt agccataagt ctactataaa taactactct ttctacatgg
     8761 ctatatgtat atttatcctt tgcatttata atacttatta aagtttttat agatgctaca
     8821 agagcatttt cctcttcatt taaatctcgt tttaattcat ctaaaacaga tacataagtt
     8881 tcaactctat ttttatggaa aaatttagct ctatataatg catcatcagc acatttaact
     8941 aaatctagct cacttttagc tttatctggg aatacagcta taccaacaga tactgtaaaa
     9001 ttagcattag gttgattttc ttgtccttca aaatatgttt tttctacttt atttctaatt
     9061 ctttctgcaa ttttagtagc ttcctcttct gaagtattag gtaaaattat tgcaaactct
     9121 tctcctccat atctagctac taaatcattt tctctagcta tattttttag aagttttcct
     9181 atttttttta atgcttcatc gcctttttga tgtccataca aatcgttata atgcttaaaa
     9241 taatctatat ccataaatat taatgacagt ttctcatttt caacctcact aatatgcatt
     9301 ttctctctta atttatcata aaaatatcta tgattataaa cccctgttaa tccatcttcg
     9361 ttaaccaagg tttgtaattc ctttatgtga tcattttcag tttttacata aaaacctaat
     9421 gtccaagctg ttaaaacaaa tactcctgct aatattaaat cattttgaaa ataaatattt
     9481 acatcagcat tccctgaaaa aatcaaatct atagctaaaa taatagctga tgacactact
     9541 gatatcaaaa ctcctttatg catgccacct tgtattgtag atgttattat aataaataaa
     9601 aataaaaatt tattattact agaattccct cctgaaacta atatagccat tataaatata
     9661 atcataaata aaattatttc tattaattgt atcttattta tatgatcttt atatttgttt
     9721 aaactagcta aactccaaag tatgtaaatt gttgataata agaatatact tattaaaatt
     9781 gataatatac tgtaatacgc taaaaattta ttgttatact ttgtaagatt aaaggtgtat
     9841 tgtaaaaaag ccatagctga aaataataat gatgatactt ttactattga taatatttca
     9901 tcaacttttt tctttttatt atcatttaca tccctaatca tattctccct ctctaatcta
     9961 gtataaaata aggccggttt aaaaccgacc tttaggtaaa ttattcatct cttaaagatt
    10021 ttggttcctc tggttggtgt gtaaaccaaa gacatgctga agtagcaact gttgttgcaa
    10081 ctactgttgt taatgcagca aataatgtta ataagttttt gtttaatttt ttcatttaac
    10141 tcatcccctc aagatttata ataatttatt tgtaaaagaa tctatagttt ttaacaatat
    10201 atttcctatg taggttagag tcatacattg ccatgctact ccaaagatta tacttaacat
    10261 aacaggctta gctaaactcc atcctgaatt ataatataaa attatactta atacttcaat
    10321 aaataaatat atagttaaaa tctttaaaga tccttttttc atgcgttttt tcttcttttc
    10381 ggtttttatt ggtttatttt tagtatctac tggagcaaat ttaaaaacta taaaatatcc
    10441 aaaaacaaaa ataactattc caataaacac tacaagctca aagttcattt ttgcaaaaaa
    10501 actacttttt attaaaaaac caatacatat tgaaattatt atgcctaata aagtacatac
    10561 attagaggat tctgaatgtg ctcctccaga atattttcta agtatgcttg ctgtaaataa
    10621 aaatatcaaa gcttctaaag ctatattaaa cactaatccc agtataagta ctgaaattat
    10681 tgatattaat gtttgtatta gagcataagt tccatactga ataatttctt ttctttcatt
    10741 atcataattt aattcactag aaactttttc agctattaac ttcgatatat tctcaatcat
    10801 tttatctcaa ctttcctttt tttgatgcta ttaaataata ataagtaaca ataaccataa
    10861 acattatagt agatggaata cctaatattg tttttgtaaa tacattttca aataaataat
    10921 caatattttc attgaaaaat ttctctataa ataataagtt taaaccttca cataaaaata
    10981 aaatcattaa tattaatatt gatgctttta tacttactat taaatctatt ttattaacat
    11041 tgaatgctaa aaatattaaa acaaatatgt tcaatattgt atgaatacca taatttatag
    11101 gtaataatct aattaaataa actactaata ccaatatcaa acttgaaatt aaatattcct
    11161 tcttttctaa ctttttatta gcgaaagcat acatggctaa catcatcgta agagcttctg
    11221 gtattcccct tactaaagtt tcaaataaag ttacttttaa cattcttcct ccgctgtcac
    11281 taagcaaata tattcctttg cttatattca tttttctcat tagttttttt aactgtaaca
    11341 tatattatta tgactacaaa aactcttata agtatatcac caatacttaa aacactatat
    11401 cctacatcta ttatgtcact taaatactta tattttactg tacttgttcc tatcatatgt
    11461 atatcattta cctttgaaat agtgttaggc ttaagatatc ctgttaaata tgataaactt
    11521 gggaaaacag gcatttcccc accgttagca gccattacaa tatgatttaa aaaagttcct
    11581 gctaaaataa ataacgatcc aattaaagca cttacatact ctttatacca aaatataggt
    11641 attaaaaaca agtacatata tatactttta aaccaaggtg cccattttac aacatcatag
    11701 ttacccataa atatacttat ctgaaagact atataaatag cttcaaaaat caaaatagga
    11761 tagattgtcc aacttttaaa gaaaggcttt aaactatatc cttttatttt tgaaattatt
    11821 aatgctataa tcactgcttc gatcattatt ttactccttt aactaattgt aaagctttaa
    11881 caaattatta aaactttatc catttaaatt aaatcatata attcatatta aatcaataat
    11941 aaacacccat tttttacaaa aaaaattgag ctttttttgt ttttaatgat aaatttctca
    12001 ttttttgttt gttttatctt ttaatttttt taatgttaat aagaacaaaa ttaaacttaa
    12061 aactccaata acaagtttta aatttttttg agaagccatt atcaccccat aactagctaa
    12121 aacttctaat aaaatagaag gtattttccc taagaatgta gatattataa atgtaaaact
    12181 attaaccttg ctcatagcag cagctaaggt tactaaacct gatggaataa agggtattaa
    12241 tcttccttca aaaattaaaa tcccaacatt tctcccttca ctctttacaa ttttatctat
    12301 taattcatac ttcccttcaa tatttcctgc aaacttattt ataccttttc tatatacttt
    12361 aaaacttatc catcccccta taacttcacc taataaagaa attaaaaatc catttatagg
    12421 accaaaaaat actatattag ctcccgtaac aaatacagag ggaactatcc caagtaatgc
    12481 tataattaca cttataaaca aacttataaa tatagctaac tcactatatt gattaaaaat
    12541 atttattaaa ctatttatat ttttctcctc ctctacttac atattaaggc tattaattct
    12601 cattttaagt atatcctcca aactgacaaa gtttcaacat attttaccta atagtaatat
    12661 tatttatttc tatctagcac aatcttatat tcaatttcct atttaattaa taaaactttt
    12721 ttaattttta gctataatat ttgaaatgaa agtctacact atatgctaaa tttcacaaaa
    12781 aaaaataact gtcttaattt aagacagtta taaaaaacta atttctcatt attatatata
    12841 ctgtatatcc aatataaaga gctaaaagaa ataccccttc atatctagta agtacttttt
    12901 cttttttctt atttaagaaa attaaagcac ctattattaa agtgacaatt ataaggaata
    12961 ttaaatctat tgataaactt gcagctattg ttatagggct tattacggat gataaaccta
    13021 atattaataa tatgttaaag gtattagacc ctaatatatt tccaagagct atgtcatttt
    13081 cacccttttt agctgccact aaagaagtaa caagttctgg aagagaagta cctattgcca
    13141 caattgtaag accaactaat ttttcactca gtcctaatcc taaggcaatt gcagaagctg
    13201 aatcaacaac tatttttcca cctaatatta ttcctataac tccaactatt gataaaatta
    13261 tatttttaac tgtgctatta ggtgacttta tgttttgctc ctcaaaatct tcaaattctg
    13321 cagttactgc tacttcacct tctcttttat tacttctctt aactgtatat attaaatata
    13381 ttatatatgt tatgcacatt aatactaata ctgctccatc tattctagat aaagagtttt
    13441 tacttcctat tagtccagta aaagtaaaaa catataaaat aaaagttgca attaaattta
    13501 ctataaaatc attttttatt atgtgcttct ttatgactaa aggagccaca atggctgtac
    13561 atcctaaagc tgctaaaaga ttaaataaat ttgatcctaa aacatttccc atggtaatat
    13621 cattgctacc ttttagtgaa gctgatatac ttactgcaag ctctggggca ctagttccta
    13681 aggatacaat agtaagtcca attataacag ctggaactcc tagcttttta gctattcctg
    13741 aagccccatc aacaaaaaga tctgctcctt taataagcaa aaggaaccct attattagaa
    13801 atatgtagtt cattaattcc tctccatttc aagtaacttt attaaatata aatatttttt
    13861 acaaatcata agtataatta tactatttat ttaaataatt tctataatat cgaaatttgt
    13921 tttcatctag ttaataattt gtctaaacat caaagcttaa ctgctctctt tcttctgtta
    13981 ttatattttc ttctaaagat gatattgtta ttcctaataa tcttaccttt ttatttttaa
    14041 aatcaactat gcttataaga tcttttacgc attccattat ttccttcttt tcttttaaaa
    14101 agtgttctaa agtaatactt cttgttatat gtttaaagtc gttaaatttc acctttaagg
    14161 ttacagtttt tcctttttta ttcaataaat ctaagctctc actaactctt tctgctattt
    14221 cctctaaaat ctctatcatt tcttctatat cttctatgtc ttctcttaag gtaatttctt
    14281 tacctataga ctttctaatc ctgtatggat ttactggcct attatctatc cctctagcga
    14341 actcgtataa tatcttgcct ctttcactaa aaatatctat aagttcttct tcagaaaatt
    14401 taagtaaatc ctctccctta aatattccta tattatttag cttattttta gttactcttc
    14461 caactccaaa aaattttcct ataggtaaat ttcttataaa atcctttgaa ttctcctctg
    14521 ttattactgt tataccatct ggttttctaa aatctgatgc catctttgct aaaaatttat
    14581 taaaagaaac tcctgctgaa gcagtaagac ctacttcttt aaatattctc tcctttattt
    14641 caagggccat taaggttgca gaacctttgc accttttact tttacttacg tcaacaaaag
    14701 cctcatctaa ggacaagggt tctacaagat tagagtattc atttaatata ttcataacct
    14761 gtctagaaac ttttttataa acctccatcc taggtcttat aaaaatagct ttaggacata
    14821 atttatatgc agttaaagag ggcatagccg aatgtattcc atattttctt gcttcataag
    14881 agcaagttgc aactacccct ctcctattgg gatccccgcc aacaatcaac ggctttcctt
    14941 tatacttagg attatctctc tgttcaatgg atgcataaaa agcatccata tctatatgaa
    15001 ttatctttct attctctttc ataaagcctc catgcaaaca tatgttcatt tttaatttta
    15061 cactaagact ttttaaattc caatattata aaagataagt tttaaacaat aaatctattt
    15121 aaaacttatc ttaaaataca ggtaattaat aaattaaaat tcctttattt aaccttttca
    15181 ggctcattat actctattcc ctttgtatcc accttaattg attctataac aacatcctca
    15241 actggtttat catttgcccc tactgaaacc ttttctattt catgaaccac atctaatccc
    15301 ttagtaactt ttccaaatgc tgcatattga ccatctaaat gtggtgcttc ttttgtaaca
    15361 ataaagaatt gacttcctgc actatcagga ttttgagacc ttgccattga taatattccc
    15421 tcttcatgct taatatcatt ttctactcca ttagaagaaa attcaccctt tatcatatat
    15481 cctggtccac ctgtacctgt tccctttgga tctccacctt gaagcacaaa tcctttaact
    15541 attctatgaa tagttaagcc atcataaaat ccactattag ctaggcttat aaagttatca
    15601 acggtatttg gagctttgtt tggatataac tctgcttcta tagttccaaa attcttaact
    15661 tcaatagtag ctacaggtaa tttatcctct ggtatatttt cctttgcctt tttacccgtt
    15721 cctccacatc ctattaaaag taaagatgcc attgtaactc ctactactat tttgctaact
    15781 ttctttaata aactcatttt tattcccctt tcttagaatt tattactaat atgttttaaa
    15841 ttatatcatc taaaccattt aaatttatgt catataagct attaatttgt ttctatataa
    15901 ttaaattttc ctaaaacaaa aaaagatatg tattcttttt tttattataa gaatacatac
    15961 ccaaaacatt tttattatac ctcaagttct tcaacttcaa cagcgaagtt cccacaattt
    16021 ttacaaacat ttatattaac taaatagctc ttgtctactt ttttatctct taataattta
    16081 ttgtaagtaa gaccttcata agctttttct atattgctta ttacaaactt tccattctta
    16141 gcataaataa acatattaaa ctctaaaaat gggaagctct tctttttttc ctctaatcca
    16201 aatttacact cttcacaagg gccatcataa taagcttttc caaagctaat cccctcacta
    16261 ctatttaata tttcaaaaag cttatctata tcaaaacctt caacatcttc attaaagaaa
    16321 taaagcaatt tatcctctga taaagtatat tctttttcat caaaagtgaa actaaacatt
    16381 taatcattcc tttctatgaa taattcacat tttattatta tctctaaaca tatttaataa
    16441 ctttaaagac aatatgtcaa actaaatttc tcttttatta aatcctttat ttctattact
    16501 ccaatataaa cttctctata gccttagcaa caccatcatt ttcatttgta tctgttatat
    16561 aatccccata ttccttaata aagtctggtg catttcccat tactacagat aatcctgcct
    16621 cttctagcat agatacatca tttccactat caccaatgca tattacttct tctaagctta
    16681 ttcctaattt atctgcaagg gctttaactg cacttccctt tgaagttcct tttttcatga
    16741 tctctataca tccaccccaa gaagcataaa ctaaatctac tccttctaaa gcttcaaatt
    16801 cctccttagc ttcttctata ttaggtccgc cttcttgttt tattattata aactttaata
    16861 tatcttctcc atgttcatca taagcatctc ttaaatcatc atacttgtag aattttattt
    16921 tttcttcttc atctacccag ctattcataa gcctatgact atcatcctct ggaatatcag
    16981 tatctgctat tagtccatct gtagaatcta cataagctaa aaacttatat ttgtttataa
    17041 tatcacatat tttaaagtaa gtttctctgt ctagtgtaga tttaaatatt acctcatcag
    17101 tatttttatc ctttatatat gaaccatttg aacagattaa tggtgcattt actcctatta
    17161 tattcccata tattctagct gacggaaaaa cccttcctgt tgatatggca atatgtactc
    17221 ctttttcaat agctttcttt aaagcctctt tatttctttc actaactttg tgatgagaat
    17281 ctaaaagcgt accatccata tctatacaaa ctaatttata attcatttaa ttctcccctt
    17341 tctaaattaa attttatata gactttcttt tttaatcaag atttaatatt ttaatataca
    17401 gcccttaaat aaaagtagct atgttataac ttaaaattct atttgttata aattctatat
    17461 aatatttttc taaagactta taaagttatc ttctaaaata aaaaacttat aaatatcttc
    17521 tgagaacctc tgaattccac taaaaattta agttcattca aatattctct tcccaatatt
    17581 tataagtttt atttacgagc tattttagta taacccttta aaattcatat tatttatttt
    17641 tatatttctc taattctaaa tctacctctg aatctgaagt tttatcttct tgtgtatttt
    17701 gtgttctata cttatttaat tcatcgtcta aacttaattc ttctaattta gcaaattcat
    17761 catctaagct ttcacctttt aattcttcta atccagcagc ataactctct tttcttgaaa
    17821 tacttctttc tatatcatca atattaattg aattactctt actttgaaca tttgcaataa
    17881 cttcatttat ttgttttgaa gcttcagcat tattaagtct agctactgct tcatctctat
    17941 aacttcttgt tttatctaat tctttttcta attccactaa ttttgctttt aaaacatcag
    18001 ctttagctct ttgtccttca tagcttgcct ttaaagactc aaatttcttg tcactatcta
    18061 atttaagttt taaagctttc ttagctaact cttcatttcc tttgctcatt gctaatctta
    18121 ctttttcatc atactgagct gactcttcct tagcttcatt cattttcttt tcagtatctt
    18181 ttaagttacc aaaaatttga gctgatgatc ttttagcttc attaaaagat ttctccatat
    18241 ctcttatttt ttgatccaat aattctactg gattctccat ttcatctaaa gctgagttag
    18301 tcttagcctt aatcatattt gatattctat taaatattcc cattttatta ccacctttac
    18361 cgacttttta tttagattta ttatactctc tcttttttac aaattcatgt cataattatt
    18421 aattttaact atcttctttt tctaattgca aatattatta aagctattaa taaaatagtt
    18481 ccaataccta taaaaggtct tcttatgaac ataaaactaa acatatttcc aaacatatta
    18541 agtatattat ttaacataag catatcaaaa aagctaaatc ctcctgaatt gctacttcta
    18601 tagcttttat ttgaactacc actagattta tcattatttt catacttatt attttcttta
    18661 gaacttcctt cttgattatt aggggctttg ctattaaact tatctgtagt tgaataatta
    18721 ttatttccct gtgtactacc tttatctgag ttactagaat ctttcccact ttctacttta
    18781 tcagagcttg aattacttcc tgatgaaggt ttattaaact tatccgtagt ggaataatta
    18841 ctatttcctt ggcttttttt cttttcacta gatgaactac taggcttaga atacttttta
    18901 ctagaattat aagaactact cttacttgaa cttctagaag aacttcctga actccttgag
    18961 ctacttctag agcctccacc tgaggatctt ccacctttag caatagtatt ttctatacca
    19021 aaggtttctg ccttaacttt tttaattgga gaaatattaa aacatccaac taaaaagaaa
    19081 aaagctataa aaaataggac taactgacca aacctcttat gttttttcac ctcttcccct
    19141 ccttaaatgt attatatatc atcattataa attaattatt aaattttgta tattttaata
    19201 gtctaaattc ataattattt gataatatat gatatcataa atatatatat tatttatatg
    19261 gtgaaggagt atagcaaatg aatgcaaagg aatttttaga taaagaaaaa aatacttatg
    19321 gtaatcttta cagtgagcta atttttgttt tagatgatat tgaagattta agcgaagatt
    19381 ctactttaaa ggaaagagca tactacaaaa atattaaaat attaaaaaaa tataatgatt
    19441 tattagataa gttatctaga gaacttccag aaaaaaaatc tttcctgtca tttttaaata
    19501 aatctaaaga aaatgaaatt ataaaagaat gtgaagattt caaaaaaact tataaagatt
    19561 ctcttgataa aatatttgta tgttctaaat gcatgtgtgt aaaatgcata agagaatgtg
    19621 gctttaaccc atgtttatca tgtaacaaaa ctggaaaggt taattattgt gataaagaaa
    19681 atactaatct tatagtattt aaaaacttta taaaacaaca atacaataat gaaactaatg
    19741 aaaatgaacc tattgaaatt ctttgtgaag tggaaatgtt agatatggac aagagtttta
    19801 gaataattaa agaagtttta agtggtgaac aatttgtgct tgagtatgtc tacaatataa
    19861 aagatggtga tttatataat tcaattgagg atgtagacct atttaatgaa ataatagata
    19921 tttatgaaag taataagata taaattaaaa taaagtattt aaacacttaa taagggcgtg
    19981 tattaaaata accttttata ttttaataca tcccttaaaa tcttatttta tatatctact
    20041 ttttatttaa tattatctta attctacaag ttttatatct aatttaatta aacaataaat
    20101 ctaatatttt ttcaccagct tttctattct ctcctttata agggaaacaa tgaatatgta
    20161 ttgctgggtt aaaattcaat tctattatgc catgattttt ttcctttgcc tcttcctcta
    20221 tattatctat taccatatct actccgcata taatggcttt aacagcctta gcagacttta
    20281 atgctacctc tttataacta ggatgtattt tgtcagtaaa gtctatactg tctcctcctg
    20341 tgcttatatt agagttttct cttaaataga ttttttctcc atttttaggg atatacttaa
    20401 agcttagtcc ttgatttttc aaaaacattt cttctatctc tcctaatttt attttttcta
    20461 aaggagtctt atatcccctt cctcttaatg gatctttatt cttttcaaaa acaagctctt
    20521 ctatggtact ttctccattc ccaattacat tggcaggttc tctatgaagt attcctacta
    20581 cctcttcccc tattacaaga aatctatatt cctttcctgt cataaattct tctataagga
    20641 ttgaactatc ctctctaaaa gctatttcaa cagctttatc atagtcttcc cttgaatatt
    20701 ctccaggaaa tatactaatt cctaaaccaa aattagtaga cttaggtttt ataactattt
    20761 tctcatcctt aaataatcta aaatctttct ttgcctcatc
//
