LOCUS       NZ_CP075970            20564 bp    DNA     linear   CON 27-NOV-2024
DEFINITION  Clostridium perfringens strain C269 chromosome.
ACCESSION   NZ_CP075970
VERSION     NZ_CP075970.1
KEYWORDS    RefSeq.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Clostridiaceae; Clostridium.
REFERENCE   1  (bases 1 to 3208157)
  AUTHORS   Jaakkola,K., Lahti,P., Virtanen,K., Lindstrom,M. and Korkeala,H.
  TITLE     Comparative genome analysis and spore heat resistance assay reveal
            niche adaptation within Clostridium perfringens enterotoxin-carrying
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3208157)
  AUTHORS   Korkeala,H. and Jaakkola,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) Department of Food Hygiene and Environmental
            Health, University of Helsinki, Agnes Sjoberginkatu 2, Helsinki
            000066, Finland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020138715.1-RS_2024_11_27
            Annotation Date                   :: 11/27/2024 04:03:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,914
            Genes (coding)                    :: 2,876
            CDSs (with protein)               :: 2,876
            Genes (RNA)                       :: 128
            rRNAs                             :: 10, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 22 of 38
            Pseudo Genes (internal stop)      :: 10 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:22:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1039580
            Orig. end                         :: 1060144
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075970.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20564
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20564
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20564
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10564](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10564)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     gene            151..1272
                     /locus_tag="KLF29_RS04410"
                     /old_locus_tag="KLF29_04375"
     CDS             151..1272
                     /EC_number="2.7.1.31"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456183.1"
                     /locus_tag="KLF29_RS04410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04375"
                     /product="glycerate kinase"
                     /protein_id="WP_224379743.1"
                     /transl_table=11
                     /translation="MKFVLAPDSFKESMTSMEACNAMERGIKKVFKDAECIKVPMADGG
                     EGTVEALVHATFGRIERVNVTGPLGNKVEAFYGVLGNGKTAVIEMASASGIQLVKREER
                     NPLITTTFGTGELIKAALDQGIKHLVIGIGGSATNDGGCGMLQALGGKLLDKDGNQIAF
                     GGGALKDIESIDLSSLDERLKEVSIEVACDVTNPLVGEKGASAVFGPQKGATKEIVKEL
                     DNNLAHYASVIKKDLGKEVSHIEGAGAAGGLGAALLLLGGELKRGIELVIKHTELAEKV
                     KGADFVFTGEGSIDDQTIYGKTPMGVATVAKENGVSTIAFSGKVGEGIEGLYEIGIKSI
                     FSIMTGVSTLDEALKEGSRNLELTTENVVRLLK"
     misc_feature    complement(1364..1382)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.1"
                     /tool="antismash"
     gene            1662..3098
                     /locus_tag="KLF29_RS04415"
                     /old_locus_tag="KLF29_04380"
     CDS             1662..3098
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480608.1"
                     /locus_tag="KLF29_RS04415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04380"
                     /product="DEAD/DEAH box helicase"
                     /protein_id="WP_060669921.1"
                     /transl_table=11
                     /translation="MKFNELNLSEDIIKALNNLGYKNPSEVQEASIPKLLKGEDIIVKS
                     QTGSGKTASFGIPVCEKINIEEKKVQCLVLTPTRELAIQVKDELSNIGRIKKVRCAAIF
                     GKQPFNEQIRELKQRVHIVVGTPGRVFDHMERGTLDLSEVNYVVIDEADKMLSMGFIDQ
                     IKDILGELRLNKTTALFSATFSKEIEEICEEYMRNPMLLEVKSKVFNRDRIEESAIEIE
                     EKNKGKALWKCLYAYHPESAIVFCNTRNKVKSVYKMLKDEGITCNELHGDMEQKKRIEV
                     MENFRDKHFKVLVCTDIAARGIHVDSIELVINYEVPMETESYVHRIGRTGRAGREGKAI
                     TFVNHYEKKYLENVEEYIGYEIKKVSLPSDEEVKAGRKIFKESQKVQLKESKKPKKEVH
                     SDIVKIHVNGGKKKKIRAFDIVGCFSNLPGLTGEDIGIIDVQDGFSYVDILNGKGDKVL
                     KIFKEVTIKGKKVKIQKARK"
     gene            3353..3811
                     /locus_tag="KLF29_RS04420"
                     /old_locus_tag="KLF29_04385"
     CDS             3353..3811
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474304.1"
                     /locus_tag="KLF29_RS04420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04385"
                     /product="hypothetical protein"
                     /protein_id="WP_003454082.1"
                     /transl_table=11
                     /translation="MKKKVAISITTLVIIFLISIVYLGLYHNDYVGENKSLNKEVISII
                     EGEEDKKLDLISLNKNIAFEWDEVYLIAPYQDVSDFFKEMNAYAPEKTYTSEINDVYML
                     AFTKYSDKLGKNKLIEYTYIPGTYIDSEKLKDMEVINGNYYYANDNLV"
     gene            4000..5376
                     /locus_tag="KLF29_RS04425"
                     /old_locus_tag="KLF29_04390"
     CDS             4000..5376
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590405.1"
                     /locus_tag="KLF29_RS04425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04390"
                     /product="MATE family efflux transporter"
                     /protein_id="WP_003480609.1"
                     /transl_table=11
                     /translation="MIITRDKRFYRLLFSIALPIAVQNLITFMVSMVDTLMVGALGEIQ
                     LSAVSIANNLFFVLTILMFGLAGGSNIMISQYWGKGNVKTIHKILAIMYRVCLLITGIF
                     IFIALFLPKYFMGIFTTDKAVIDFGASYLRIVCIGYLFYSITNCTIMMLRSVKTVSISI
                     IVYTASLVVNSILNWIFIFGNLGAPELGIRGAAIATVCARITEFSIVLVFMFIYERKIG
                     LKIEHLLKLDKEILKDYVGLCTPVLCNELLWAIGASMISVIVGRMGTEVVAANSINGVA
                     HQFVTVFIFGMSNATAVIIGNTIGEGKKEKAKEYAYSIGIFSVVMGCISGLMILLIKPF
                     VVNFYNVSYSTKLIAMEIMTVTSGIIVFQSLASNFMMGVLRGGGDAKFVLINDLIFMWL
                     VAIPGGFFVAFVLELPVALVFLVIKCDEILKSLTSVYRVISGKWVNDVTKDYEFEEVKC
                     "
     gene            5483..5656
                     /locus_tag="KLF29_RS04430"
                     /old_locus_tag="KLF29_04395"
     CDS             5483..5656
                     /GO_function="GO:0003690 - double-stranded DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454088.1"
                     /locus_tag="KLF29_RS04430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04395"
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="WP_003454088.1"
                     /transl_table=11
                     /translation="MSHKNNKNSKDNKKTSQKTKKEVEKMKNETANEIGYSKESRKLGK
                     DKTRNNPYMGPQ"
     gene            6001..6180
                     /locus_tag="KLF29_RS04435"
                     /old_locus_tag="KLF29_04400"
     CDS             6001..6180
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010084.1"
                     /locus_tag="KLF29_RS04435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04400"
                     /product="hypothetical protein"
                     /protein_id="WP_061426888.1"
                     /transl_table=11
                     /translation="MGYIIILLSPILGLIFSEIYVFLASFMSWISADGFVTSTSVMFAI
                     LGLIIVLNDKEKTK"
     gene            complement(6290..7450)
                     /gene="alr"
                     /locus_tag="KLF29_RS04440"
                     /old_locus_tag="KLF29_04405"
     CDS             complement(6290..7450)
                     /EC_number="5.1.1.1"
                     /GO_function="GO:0008784 - alanine racemase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="alr"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477225.1"
                     /locus_tag="KLF29_RS04440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04405"
                     /product="alanine racemase"
                     /protein_id="WP_003455853.1"
                     /transl_table=11
                     /translation="MDICVRPVWAEIDLDIIANNMKEIRNLVGEKEIIAVVKANAYGHG
                     ALDIASTLLENGASRLAVAIITEADELRDAGITAPIMILGYTPINFAENLINNEIEQTV
                     YDVEYAKELSDFALKLGKKAKIHIAIDTGMGRIGFLPNEEGLNKVLEICSLPGVEVVGL
                     FTHFSTSDEKDKTYTYEQFSKLTAFNKALEDNGIHIPLKHASNSGAIMDLPETYLDGVR
                     CGIISYGYYPSEEVKKENLKLKPALTLKTNVAFVKELDEDMYVSYGRTYKTEKKSKIAT
                     LPIGYADGYSRLLSGKAKVIIKGQFANVIGRVCMDQCMIDVTHIEDVKIGDEVILLGEE
                     NGLKFDANDMAEIMGTINYEILCMISHRVPRIYKKNNEIVKVRNYI"
     gene            7991..8560
                     /locus_tag="KLF29_RS04445"
                     /old_locus_tag="KLF29_04410"
     CDS             7991..8560
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455714.1"
                     /locus_tag="KLF29_RS04445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04410"
                     /product="N-acetyltransferase"
                     /protein_id="WP_003480612.1"
                     /transl_table=11
                     /translation="MLDKSIEFFKIILKREKGKELKDETLPEGFRFVKFKKGDEKAWAE
                     IEKSVLEFENAKDGEEYFKNKYLPYIDELERRTIFIENNKGEKIATFTAWWRYTGERRH
                     PFMEWVAVKPEYQGKGLGKALISEGVKLMIAIEGDCDMYIPTQTWSYKAIRLYRWAGFE
                     FETEEKFPGGIKNETLEGIKVIKNLI"
     gene            complement(8612..9706)
                     /locus_tag="KLF29_RS04450"
     CDS             complement(8612..9706)
                     /EC_number="2.7.13.3"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010087.1"
                     /locus_tag="KLF29_RS04450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_224379744.1"
                     /transl_table=11
                     /translation="MGNSSVATLILHLVMLIIGGLIYKDDFLAAAIGFSLIYSFIVITL
                     IISSFLYNVLSQNISLINKSNIFFITIMYFPQYILAFLVLYKKKSIYSLYKTIKSRNSS
                     IIYLILFTIVLDFIASFNAIINAKDNPIFTEIILTLLFIFIIFIIIYFSNIEKKSKEIE
                     LLNSNLEQNISSLRKLKHDYGSQISYLYGLHLMGNYEKLGELLKNIIDGNDNIINNIVV
                     SNDTSIISMIVNTINLKDITVFIDENADLLETNVNELDFQRIVSNILRNSIEALDGSGS
                     IKINTYYSFNYFVLKIQNNGPEIPDKILNKIFDSGFTTKENKESNNGFGLAIVKELVES
                     YNGKIQVSSTDEFTEFTIYLPTIK"
     gene            complement(9877..9999)
                     /locus_tag="KLF29_RS15210"
     CDS             complement(9877..9999)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453910.1"
                     /locus_tag="KLF29_RS15210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_003453910.1"
                     /transl_table=11
                     /translation="MRKSILSMLALLTTLTINASTTSFATIGLEEMPESMKKSR"
     misc_feature    9993..10007
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 24.71"
                     /tool="antismash"
     gene            complement(10001..10564)
                     /locus_tag="KLF29_RS04455"
                     /old_locus_tag="KLF29_04420"
     CDS             complement(10001..10564)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474343.1"
                     /locus_tag="KLF29_RS04455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04420"
                     /product="accessory gene regulator AgrB"
                     /protein_id="WP_003453984.1"
                     /sec_met_domain="AgrB (E-value: 3e-31, bitscore: 105.6,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKFLKSAIEYLAKDLDLDKELLEQIQYVVIVLTFEFIKCISVIL
                     IAGILGYFKESLIVILSMCFIKPFIGGYHEDSQLKCFIATLIITTSIIMLVTFNNLNLF
                     SIVILNLFSIFSIYNKAPVIDSRFPLTKEHLIKKNKILSVTNSSILFLITLIFFKISWI
                     SQTITWTLLIQTLLLFNKYKREDS"
     misc_feature    10676..10691
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.79"
                     /tool="antismash"
     gene            10926..12701
                     /locus_tag="KLF29_RS04460"
                     /old_locus_tag="KLF29_04425"
     CDS             10926..12701
                     /EC_number="3.4.11.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004177 - aminopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1172: methionine aminopeptidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458836.1"
                     /locus_tag="KLF29_RS04460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04425"
                     /product="aminopeptidase P family protein"
                     /protein_id="WP_195390908.1"
                     /transl_table=11
                     /translation="MKVTERLEKLRKIMKDKGIDYYIIHSEDAHQSEYVCEHYRGRAYM
                     SGFTGSAGTLLVGLENAILWTDGRYFIQALEELKGSGIEMFKMRIPGWPSLLEWLKENA
                     KAGETIAFDGKVFSVGEYKDFKKLEEENNINIKIDEDLLDEVWKERPSLPKEKAFLHEV
                     KYCGKSAKEKLREVREEMKKLGANNYIIASLDDIAWLYNIRGNDVKCNPVVLSYALVKE
                     NEAYLYVDKSKFTSKMEEELLNEGVTLKSYDEIGNAISNLEGKILIDPNKISAYLYECI
                     KDKNNIVEFGNITTKFKAIKNEVELDNLRKCQVRDGVAMVKFMKWLKDNIGKIEISEIS
                     ASDKLEELRSLDKLFKGISFETIAGHKEHGAMMHYSATKESDYTLEPRGFLLIDSGGQY
                     LDGTTDITRTFVLGELTEEERKDYTLVLKGHIGLMRAKFLKGATGSALDIKAREPLWNE
                     GIDYKCGTGHGVGFFLNVHEGPQSISPVPNKVALEPGMIITNEPGVYREGKHGIRTENT
                     MVVVKDTYSEEFGEFYKFDTISLCPIDLAGLDISLINEEEKAWLNNYHKKVYYLLSPYL
                     DEEEKEFLKNETREI"
     misc_feature    complement(12733..12747)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.87"
                     /tool="antismash"
     misc_feature    12734..12748
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.27"
                     /tool="antismash"
     gene            13074..14201
                     /locus_tag="KLF29_RS04465"
                     /old_locus_tag="KLF29_04430"
     CDS             13074..14201
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477233.1"
                     /locus_tag="KLF29_RS04465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04430"
                     /product="aminopeptidase"
                     /protein_id="WP_195390907.1"
                     /transl_table=11
                     /translation="MADQRLNKLAKLLVNYSTGVKEGDFVFVSCNEVANPWLTEVVKEA
                     TKAGAHVEYILESEEAKEARLKFSTKDQLLSGNLIMETMLEKADVWLSAWGTRNTRAFS
                     NIDSEKIKDSRAGEKGWRKFYSGRMGDGSLRWCGTQFPTYADAQEASMSFSEYEDFVYR
                     AGLLDHEDPVAEWNRVSKEQERWVKYLDTKKELHILAEGTDIKVSVEGRKWINCDGRVN
                     FPDGEIFTSPVENKINGHITFSFPGIYAGKEIEGIELEVKDGKVVSYKAKKGEDLLKAL
                     LETDEGASYFGEVAIGTNYGIKKFTRNMLFDEKIGGTVHMAIGDSMPEAGGKNRSSLHW
                     DMLCDMRNGGRIYADGELFYENGEFKKEILEKYNL"
     gene            14305..14748
                     /locus_tag="KLF29_RS04470"
                     /old_locus_tag="KLF29_04435"
     CDS             14305..14748
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465947.1"
                     /locus_tag="KLF29_RS04470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04435"
                     /product="N-acetyltransferase"
                     /protein_id="WP_011010092.1"
                     /transl_table=11
                     /translation="MSNIYEFKKEDLNECAELFVKTFSKEPWNEPWDFENAKKRLNDVV
                     LTPGFRGAVLRNDEKIEGVILGNLEQWYDGEHFCVKEFFVDSSSQGKGTGKKLLNALED
                     ILMEKEVGVIHFWTMKGSTAEAFYNKRGYEIPKELIMMRKKLK"
     gene            complement(14931..15596)
                     /locus_tag="KLF29_RS04475"
                     /old_locus_tag="KLF29_04440"
     CDS             complement(14931..15596)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455751.1"
                     /locus_tag="KLF29_RS04475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04440"
                     /product="YitT family protein"
                     /protein_id="WP_003455751.1"
                     /transl_table=11
                     /translation="MESKKKILRSVLLIALGSSILAFGSYNFNYQNNVTEGGVLGLLLL
                     VKNVFDISPSITNLIIDFSLFAIGSKFFGKKFLACSIFATFCFSVSYRFWESIGFLTPN
                     FTNNMLVASVLAGIGVGVGAGIVLRGGGASGGDDVIALLGNKFTPLKVQHVYMLTDAIV
                     LLMSLVYLDFKQIFFSIIAVCISGKLISVIYEYKNDGIDTKDENKEAEVLEKNGSLTV"
     misc_feature    15953..15964
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            16068..16610
                     /locus_tag="KLF29_RS04480"
                     /old_locus_tag="KLF29_04445"
     CDS             16068..16610
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465963.1"
                     /locus_tag="KLF29_RS04480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04445"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_110027123.1"
                     /transl_table=11
                     /translation="MKVIVRKELEQDHKTVELLVEEAFKDAEYSDHMEHVLVSKLRNGE
                     NFIDGLSLVAEREGEIVGHILLTKANIENETEKYETLALAPVSVLPKYQGIGIGAELIR
                     EGMRMAKELGYDSIIVLGHDKYYPKFGFKKAGDFGIKAPFEVPEENFMAIELNEEALKN
                     ISGVVRYANEFLADNNK"
     gene            complement(16863..17618)
                     /locus_tag="KLF29_RS04485"
                     /old_locus_tag="KLF29_04450"
     CDS             complement(16863..17618)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010094.1"
                     /locus_tag="KLF29_RS04485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLF29_04450"
                     /product="DUF1189 family protein"
                     /protein_id="WP_195390767.1"
                     /transl_table=11
                     /translation="MNLFKRFYISLFTPKRYKELIKTSGISTFFYLLILSLLLGLLAFS
                     RNYTELNRFLISRKSAFENKIPPFEIKDGVLDLKDSNFVVFQEENWTFVLNDKEPAKDL
                     LKDYESGIAFGNESLIIKYDNNKITEANYSNMNLSLNDNDLKNSINSLERTFGNTYLSL
                     MVIIFIAFSYIITLILAIITKIIFSIRNRAVGFLEILKLSTYGITPCLVLLALLNLLSL
                     NMLITLPLSFLVSIIYVYFGINYLNKNTF"
     misc_feature    complement(17699..17713)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 23.65"
                     /tool="antismash"
     misc_feature    18632..18642
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    19215..19229
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 23.2"
                     /tool="antismash"
     misc_feature    20022..20036
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 15.62"
                     /tool="antismash"
ORIGIN
        1 aataaagaaa atttttaata aggtttaatt taaacttagt atgaaattta ttaactttta
       61 agaattatga tttaaaaaag agtttttagt aaaagcacag tggattttaa aatgaaaagt
      121 taagataaaa tctagtagga ggaaataaag atgaaatttg tattagcacc agattcattt
      181 aaggaaagta tgacttcaat ggaagcctgt aatgctatgg aaagaggaat taaaaaggtc
      241 tttaaagatg cagaatgcat aaaggttcca atggctgacg gtggagaggg aactgtagaa
      301 gccttagttc atgctacctt tggaagaatt gaaagagtaa atgttactgg acctttagga
      361 aacaaggtgg aagcttttta tggagtttta ggtaatggaa agactgctgt tatagaaatg
      421 gctagtgctt ctgggattca acttgttaag agagaagaaa gaaatccttt aataacaaca
      481 acttttggaa ctggagagct tataaaggct gcattagatc aaggaataaa acacttagtt
      541 ataggaatag gtggaagtgc cactaatgat ggaggatgtg gaatgcttca agctttaggt
      601 ggaaaacttt tagacaagga tggaaatcaa atagcctttg gtggaggagc attaaaggat
      661 atagaaagta ttgatttaag ttctttagat gaaagattaa aggaagtatc aatagaagta
      721 gcttgtgatg taactaatcc attagtagga gaaaaaggag cctcagcagt atttggtcct
      781 caaaagggag caactaaaga gattgttaaa gaattagata ataatttagc tcattacgct
      841 tcagtaataa aaaaagacct aggaaaagaa gtttcacaca tagaaggggc tggtgctgct
      901 ggaggattag gtgccgcttt attactatta ggtggagaat taaaaagagg tatagagctt
      961 gtaataaaac atacagagtt agctgaaaag gtaaagggag cagactttgt atttactggt
     1021 gaaggaagta tagatgatca aactatatat ggaaaaacac caatgggagt tgctactgtg
     1081 gctaaggaga atggagtttc tacaattgcc ttttcaggaa aagttggaga gggaatagaa
     1141 ggcctttatg aaataggtat aaagagtata tttagtataa tgactggagt ttcaacttta
     1201 gatgaagctt taaaagaagg aagtagaaat ttagaattaa caactgaaaa tgtagttaga
     1261 cttttaaaat aaagtttatg actttataaa gttatattta taaagtgata gagtgaagat
     1321 aaagaattaa acaaaattta atacttactt acaatagata gtttaaggtg aacataagca
     1381 ataaaaaata tattgtttgt gttcactttt ttattataaa gaaaattctt tataattaag
     1441 atattttaaa atatataagt taataaagga gaaaacatag tattatatta tttctttaat
     1501 tttaagaatt ttaactgttt ccctctttat gatatatgga aaaatgtaat gttatcttat
     1561 tttcttagga ttttaaaaat taaaattagt agaaaaaatg aatatatggt ataactataa
     1621 gaagactaat aaagttttaa aaaaggagat aaaaacaata tatgaaattt aatgaattaa
     1681 atttaagtga ggatattatt aaagcgctta ataatttagg atataaaaat ccatcagagg
     1741 ttcaggaagc ttctattcca aagttattaa agggtgaaga tataatagtt aaatctcaaa
     1801 ctggtagtgg taaaactgca agttttggaa tacctgtttg tgaaaagata aatattgaag
     1861 aaaaaaaggt tcagtgtcta gttttaacac caactagaga gttggcaatt caagttaaag
     1921 atgaactttc taatatagga agaataaaaa aggttagatg tgcggctata tttgggaaac
     1981 aaccttttaa tgagcaaatt agagagctta aacaaagagt acatatagta gtaggaactc
     2041 caggaagagt atttgatcat atggaaagag gaactttaga cttaagtgaa gttaactatg
     2101 tagttataga tgaagctgat aaaatgcttt caatgggatt tattgatcaa ataaaggaca
     2161 tattaggaga attaagatta aataaaacca ctgcattatt ttctgctaca ttttctaagg
     2221 aaatagagga aatatgtgaa gagtatatga gaaatcctat gcttttagag gttaaatcaa
     2281 aagtatttaa tagggataga atagaagaaa gtgcaattga gatagaggag aaaaataagg
     2341 ggaaagcttt atggaaatgc ctttatgctt atcatccaga gagtgctata gtattttgta
     2401 atacaagaaa taaggttaag tctgtttata aaatgcttaa ggatgaggga ataacttgta
     2461 atgaacttca tggagatatg gagcaaaaga agcgtattga ggttatggaa aactttagag
     2521 ataagcactt taaagtatta gtttgtactg atatagctgc tagaggaatt catgtagata
     2581 gcatagaact tgttataaac tatgaggttc caatggaaac agaaagctat gttcatagaa
     2641 taggaagaac tggtagagca ggaagagaag gaaaggctat tacctttgta aatcattatg
     2701 agaagaaata tttagagaat gttgaagaat atattggtta tgaaataaaa aaagtaagtc
     2761 ttccttctga tgaagaagtt aaagctggaa gaaaaatatt taaggaaagt caaaaggttc
     2821 aattaaaaga gagtaaaaaa cctaagaaag aagttcatag tgatatagtt aaaatacatg
     2881 ttaatggcgg aaagaaaaag aaaataagag cctttgatat tgttggatgt tttagtaact
     2941 taccaggttt aacaggggaa gacattggaa taatagatgt tcaagatggt ttttcttatg
     3001 ttgatatatt aaatggaaaa ggtgataaag ttttaaagat ctttaaggaa gttactataa
     3061 agggtaaaaa agtaaaaata caaaaagcaa gaaaataaaa ttattatata cgatttaaga
     3121 taaaatacat ttaagttata tgttgtattt aaagtacatg taaaaataat agtgacttat
     3181 aagtttttac tttgaaaata ttatgattaa tttaattata aaagtagtta taatgttaaa
     3241 ttgttttgaa gtatataatg accatgtaat atgtggtaaa attaaagtaa gatatagtaa
     3301 aatatatctt acttttttta aaaggaaatt ttatttttga gaggtggtct gtatgaagaa
     3361 aaaggttgct atctctatta caactttagt aataatattt ttaatatcta tagtatatct
     3421 agggttgtat cataatgact atgttggaga aaataagtca ttaaacaaag aagttataag
     3481 tattatagaa ggtgaagagg ataaaaagct agatttaatt tctttaaata aaaatatagc
     3541 ttttgaatgg gatgaggttt atttaatagc tccataccaa gatgtaagtg acttttttaa
     3601 agagatgaat gcttacgcac cagaaaaaac atacacctca gaaataaatg atgtgtacat
     3661 gctagctttt actaagtatt ctgacaagct agggaaaaac aaactaatag aatatactta
     3721 tataccagga acttatattg attctgaaaa attaaaagac atggaagtta taaatggaaa
     3781 ttattattat gcaaatgata atttagttta gattttaaag ctctaagttt aaaacttagg
     3841 gcttttttta attaagaatt aatttgaaaa taaaaatatt aataaaattg ctttaatatt
     3901 catatattgt taaatttaaa cacatattat aagttagtac agaatgctat aatgtacatt
     3961 gacttttata agaatatatt tttagtaagg gggctaatta tgattataac tagagacaaa
     4021 agattttata ggttactttt tagtattgct ttgccaatag cagtacaaaa tttaataacc
     4081 tttatggtaa gtatggtaga tactttgatg gttggagctt tgggggaaat tcaactatca
     4141 gcagtttcta tagctaataa tttatttttt gtacttacaa ttttaatgtt tggactagct
     4201 gggggatcta atattatgat ttctcagtat tggggaaagg gaaatgttaa aacaattcat
     4261 aagattttgg ccataatgta tagggtatgt ttattaataa ctggtatttt catatttata
     4321 gcattatttt taccaaaata ctttatggga attttcacaa ctgataaggc tgttatagat
     4381 tttggagcaa gttatttaag aattgtgtgc ataggatatt tgttctattc cataacaaac
     4441 tgtacaataa tgatgcttcg ttcagtaaaa actgttagca tatcaattat agtttatact
     4501 gcttctttag tggtaaattc aatattaaac tggattttta tatttggaaa tttaggtgct
     4561 cctgaacttg gaataagagg agctgccata gctactgtct gtgctagaat tactgagttt
     4621 tcaatagttt tagtgtttat gtttatatat gaaagaaaga ttggtcttaa gatagaacat
     4681 ttattaaagc tagataaaga gattttaaaa gactatgttg gattatgtac tcctgtttta
     4741 tgtaatgaat tactttgggc aattggagct tctatgatat ctgtaatagt cggtagaatg
     4801 ggaacagagg ttgtagctgc taatagtata aatggagttg cacatcaatt tgtaacagtt
     4861 tttatatttg gtatgtctaa tgctacagca gtaattatag gaaataccat aggagaaggt
     4921 aaaaaagaaa aagcaaagga atatgcctat agtataggga ttttcagtgt tgtcatggga
     4981 tgtatatcag gacttatgat tttacttata aagccatttg ttgttaattt ctataatgta
     5041 tcttattcaa caaaattaat agctatggaa ataatgactg taacttcagg aataatagta
     5101 tttcaatctc ttgcaagtaa ttttatgatg ggtgtactta gaggtggtgg agatgctaag
     5161 tttgtcttga taaatgatct tatttttatg tggcttgttg ctattccagg tggatttttt
     5221 gtagcctttg ttttagaact tccagttgcc ttagtattct tagttataaa atgtgatgaa
     5281 atattaaaat ctcttaccag tgtatatagg gtaattagtg gaaaatgggt aaatgatgta
     5341 actaaggatt atgaatttga ggaagtaaag tgttagtagt ttttaggaaa tagtaaacag
     5401 ttttggaaag ttgttttaag gatatatgag ttattaaagt taaatactaa ttaagaactt
     5461 aatttgaaag gagtgtttaa ctttgtctca taaaaataat aaaaacagca aagataacaa
     5521 aaagactagt caaaaaacta aaaaagaagt agagaaaatg aaaaatgaaa ctgctaatga
     5581 aataggatac tctaaggaat caagaaaact tggaaaagat aaaactagaa ataatccata
     5641 tatgggacca caataattaa aaaataataa aattgcagca aaatataata atggagagct
     5701 ttcttcaaaa gaatccataa ctttaagaac tgaaagataa aataaaaatt aaataattat
     5761 atttttttat atgaaaagaa gctgtattaa aaagctaagt acaattaagt ttaatttatt
     5821 taactatatt tatacagctt cttttgtttt ttattagaat tttattttgt aagggagcat
     5881 atatataata ggggaggttt atttaaacag aaattaatta ttttaaagat ataataaaaa
     5941 gaaaaaactt gtttaataat agatttttag aaaaataggt ttaattatag gagaggatta
     6001 atggggtata ttataatttt attaagtcca attttaggat taattttttc agaaatatat
     6061 gtttttttag cttcatttat gtcatggata tctgcagatg gttttgttac atccactagt
     6121 gtgatgtttg ctattttagg acttataata gtgttaaatg ataaggaaaa aacaaaataa
     6181 aaattagtgc tataaataat tatgattaaa ttaggttagg gtttatttaa attttaatga
     6241 ctaaataaga gagtgtatta aaataacttt taatacactc ctatttttat taaatatagt
     6301 ttcttacctt aactatttca ttattctttt tatatattct aggaactctg tggctgatca
     6361 tacaaagtat ttcgtagttt attgttccca taatttcagc catatcatta gcatcaaatt
     6421 ttaatccatt ttcttcccct aaaagaatta cttcatcacc aattttaaca tcttctatat
     6481 gagttacatc aatcatacat tgatccatac aaactcttcc tataacatta gcaaattgac
     6541 cctttattat aactttagct tttccactaa gaagtcttga atatccatca gcatatccta
     6601 ttggaagagt ggcaatttta cttttctttt cagttttata agttcttccg tagcttacat
     6661 acatatcttc atctaactct ttaacaaagg ctacattagt ttttaatgta agtgctggtt
     6721 taagttttaa gttttctttt tttacttctt cagatggata gtatccataa gaaataattc
     6781 cacatctaac tccgtctaaa taagtttctg gtaaatccat aatagcccca ctattagaag
     6841 catgttttaa aggaatgtgt attccattgt cttctaaagc tttattaaaa gcagttaatt
     6901 ttgaaaattg ttcataagta taagttttat ctttttcatc tgatgttgag aagtgagtaa
     6961 ataagcctac tacctcaact ccaggtaaag agcatatttc taaaacttta tttaatccct
     7021 cttcatttgg aaggaaacct attctgccca ttcctgtatc tattgcaatg tgtattttag
     7081 cttttttgcc aagttttaag gcaaagtcag ataattcctt agcatattcc acatcataaa
     7141 ctgtttgttc aatttcatta ttgattaaat tttcagcaaa atttatagga gtatatccta
     7201 aaatcataat aggtgcagtt attccagcat ctctaagttc atcagcctct gtgattatag
     7261 ctactgccaa tctagaagca ccattttcta aaagagtgga agctatgtct aaagctccat
     7321 gaccataagc attagcctta acaacggcta ttatttcctt ttcaccaact aggtttctta
     7381 tttctttcat attatttgct attatatcta aatctatttc agcccataca ggtctaacac
     7441 atatatccat tttaaaacac ctcaaattaa ttacagcttt tattactttg atttaattaa
     7501 tttcaactta aattaataag ttgtagctaa attattatgc tcatataaag tattacatat
     7561 aaatcaccat attttttatt aaaaaactta actacatata ctattataag atattgtagc
     7621 ataaagtgaa gaaccaccat aattgtaatt tttatatgga gtggtttcaa tattaagaat
     7681 ctgtcataat tttttataag tgagttcaat aatgttataa ttaatatcta caaattaata
     7741 gggatttctg tgaatatata aatactaggt tgaaaaggta tttatttaat taaattatta
     7801 ttttctttgg atcaattaat atctagatat aaagaatgaa aaaagcagtg taatattaaa
     7861 attataagtt taatgataaa attaaatata gaaaggtata ataaattact tatagtctta
     7921 ttaatgagta gctataagga taaaaattaa gtttcaatag gttatatggt ttatctggag
     7981 ggagatttaa atgttagata aaagtataga attttttaaa ataattttaa agagagaaaa
     8041 gggaaaagaa ttaaaagatg aaactttacc agaaggtttt agatttgtta agtttaaaaa
     8101 aggtgatgaa aaagcttggg cagaaataga aaaatctgta ctggaatttg aaaatgctaa
     8161 agatggagaa gaatatttta aaaataaata tttaccttat atagatgagt tagaaagaag
     8221 aactatattt atagaaaata ataagggaga aaaaattgct acatttacag cttggtggag
     8281 atatacaggg gaaagaagac atccatttat ggagtgggtt gctgtgaagc ctgaatatca
     8341 aggaaaagga ttaggaaaag ctcttatctc agaaggagtt aaacttatga tagctattga
     8401 aggagattgt gatatgtaca taccaactca gacttggagt tataaagcaa ttaggttata
     8461 tagatgggca gggtttgaat ttgaaactga agaaaagttt ccaggtggaa ttaaaaatga
     8521 aaccctagaa ggtattaaag taataaaaaa cttaatataa ttataaaata aagtttaaaa
     8581 agaaacagag gatagaactc tgtttctttt cttattttat tgtaggtaag tatattgtaa
     8641 attcagtgaa ttcatcagta ctacttactt gaatttttcc attatatgat tctactaact
     8701 cctttactat agctagtcca aatccattat tactttcttt attttctttg gtggtaaatc
     8761 cactatcaaa tattttattt aatattttat ctggaatttc aggaccatta ttttggattt
     8821 ttaacacaaa ataattaaaa ctataataag tattaatttt tattgaacca ctaccatcta
     8881 aagcttcaat agaatttctt aatatattag aaactatacg ttgaaagtct aattcgttta
     8941 cattagtttc taataaatca gcattttcat ctataaatac agtaatatct tttaaattaa
     9001 tagtatttac tatcattgat ataattgaag tgtcattaga tacaacaata ttattaataa
     9061 tattatcatt cccatcaatt atgtttttta ataattctcc taatttctca tagttcccca
     9121 taagatgtaa tccatataaa taagaaattt gagagccata atcatgtttt aattttctta
     9181 atgagctaat attctgttct aaatttgaat ttagtagttc aatttctttc gattttttct
     9241 ctatgttaga aaaatatatt attataaata ttataaatat gaataataaa gttaatatta
     9301 tttctgtaaa tataggatta tcctttgcat taataatagc attaaatgaa gctataaagt
     9361 ctagtactat tgtaaagagt attaaataga taattgagct attcctagac tttatagttt
     9421 tgtaaagtga gtatatactt ttctttttat ataaaactaa aaaggcaagt atatactgtg
     9481 gaaaatacat tatagttatg aaaaatatat tacttttatt tattaatgat atattttggg
     9541 ataagacatt atatagaaaa gatgaaatta ttaaggttat aacaatgaat gaataaatca
     9601 aactaaatcc aatagcagca gctaaaaaat catctttata tattagacct ccaatgatta
     9661 acattactaa atgaagaatt aatgttgcaa cactggaatt tcccataaaa aaagttgtta
     9721 gtgatatcat tgtccacaaa aggaatatat aaaataacat ctttgacttt ggttttttgc
     9781 aatcttcttt actacataaa tttattgtgc ttataaataa tacacattgt aaaaaagtgt
     9841 ttatcgtatc agctaaaaat atagttttat tcatttttat ctagattttt tcatagactc
     9901 aggcatttct tctaatccta tagtagcaaa agaagtagtt gaagcgttga tagttaatgt
     9961 agttaataat gctaacattg ataatattga ttttctcatt ttaagaatcc tcccttttat
    10021 acttattaaa taatagtaat gtttgtatta ataaagtcca cgttattgtt tgagaaatcc
    10081 atgaaatttt gaaaaatatt aaggttatta aaaaaagaat acttgagttt gtaacactta
    10141 atattttatt ttttttaatt aaatgttctt tagtcaatgg aaatctacta tctattactg
    10201 gagctttatt ataaatacta aaaatactaa acaaatttaa aataacaatg ctaaatagat
    10261 ttagattgtt aaaagtaaca agcatgatta tacttgttgt aataattaat gttgcaataa
    10321 aacattttaa ttggctatct tcatgatatc caccaataaa tggctttata aagcacatgc
    10381 taagaattac tattaagctc tctttgaaat atccaagtat tccagcaatt agaattacag
    10441 aaatacattt aataaattca aaggtcaata caattacaac atattgaatt tgctctaata
    10501 attctttgtc caagtcaaga tcttttgcta aatactctat agcagatttt aaaaattttc
    10561 tcataaagaa tctctcttat ctatttttta taaacattaa aataaatgta atatgcttac
    10621 attataataa aaaaataatt gatttacata agataattcc ttttttaaag gtatatttac
    10681 aattttttca taatgtagcc aatatatatg ttttatattg taattttata agggattatg
    10741 taatttttta agagaactta ttaataacat taataagtta tacttataaa gttaattaat
    10801 ttttattaat tggtattttt aggagaatat ttttgtgtta ctattattga aagcagaaaa
    10861 ttaaattgag gtttgaaaag ttaaatcaaa ttacaattta gattaaaaaa tagggggcta
    10921 taattatgaa ggtgactgaa agattagaaa aattaagaaa aattatgaag gataaaggaa
    10981 ttgactatta tataattcat agtgaagatg ctcatcaaag tgaatatgta tgcgaacatt
    11041 acagggggag agcatatatg tcaggtttta cagggtcagc aggaacttta cttgttggtt
    11101 tagaaaatgc tattttgtgg actgacggta gatatttcat acaagcttta gaggagttaa
    11161 agggttctgg aattgaaatg tttaaaatga gaattccagg atggccaagc ttattagaat
    11221 ggcttaaaga aaatgcaaag gcaggagaaa ctattgcttt tgatggaaag gtattttccg
    11281 taggagaata taaagatttt aaaaaattag aagaagaaaa taatattaat ataaaaatag
    11341 atgaggacct tttagatgag gtttggaaag agagaccatc tcttcctaag gaaaaggcat
    11401 ttttacatga agttaagtac tgtggaaaaa gtgcgaaaga aaaattaaga gaagttagag
    11461 aggaaatgaa aaagctaggc gctaataatt atattatagc ttctttagat gacatagctt
    11521 ggctttataa tattagagga aatgatgtta aatgcaatcc tgtagtttta agctatgcct
    11581 tagttaaaga aaatgaagca tatctttatg tagataaatc aaagttcact tctaaaatgg
    11641 aggaagaact tttaaatgaa ggggttactt taaaatcata tgatgaaata ggaaatgcta
    11701 ttagtaattt agaagggaag attttaattg atccaaataa gataagtgct tatttatacg
    11761 agtgcattaa ggataaaaat aatattgtgg aatttggaaa cataacaact aagtttaagg
    11821 ctattaagaa tgaagttgaa ttagataact taagaaagtg tcaagtaaga gatggagtag
    11881 ccatggttaa gtttatgaaa tggcttaagg ataacattgg aaagatagaa ataagtgaaa
    11941 tatcagcttc agataagtta gaagaactta gaagtttaga taagttattt aaaggaatta
    12001 gttttgaaac catagcaggg cataaagaac atggtgctat gatgcattat tcagcaacta
    12061 aagagagtga ttacacttta gaaccaagag gatttttatt aatagattca ggtggacaat
    12121 acttagatgg aactacagat ataacaagaa cttttgtttt aggagaatta actgaggaag
    12181 agagaaaaga ttatacttta gttttaaaag gacatatagg ccttatgaga gctaaattct
    12241 taaagggagc tactggatca gcccttgata taaaagctag agagccatta tggaatgaag
    12301 gaattgatta taaatgtgga acaggtcatg gagttggatt tttcttaaat gttcatgaag
    12361 gaccacaaag cataagccca gtaccaaata aggttgcctt agagccagga atgatcataa
    12421 ctaatgaacc tggagtttat agagaaggaa aacatggaat aagaacagag aatacaatgg
    12481 tagttgttaa agatacttat tcagaagagt ttggagaatt ttataagttt gatactattt
    12541 cactttgtcc aatagactta gcaggattag atataagttt aataaatgaa gaggaaaagg
    12601 cttggctaaa taattatcat aaaaaggttt attatttatt atcaccatat ttagatgaag
    12661 aggaaaaaga attcttaaag aatgaaacaa gggaaatata atttaacata tgaatagatt
    12721 tataaaagaa gatggaataa ttcttccacc ttcttttata tttatatagt ttccaaattt
    12781 ttataggagt ataataaaat taatagataa aacttatgaa atagatataa acaaatgggt
    12841 gattttatgg agagagaaaa ggtttgtcta aaggaaagct tttatctaaa aagagaataa
    12901 aggtattatt tataattttt attttttaaa tttaaatatt aaagaaagaa aaagattaag
    12961 ataagattag aaaattagct ttaaaaattt attgttatta ataaaaaaat agtaagttaa
    13021 aatagagttc aaaaattaaa atatataaat ttgtatgttt aggagggata tatatggcag
    13081 atcaaagatt aaataagtta gctaaactgc ttgtaaatta ttcaacagga gttaaggaag
    13141 gagactttgt ttttgtatct tgtaatgagg ttgcaaatcc ttggcttact gaggtagtaa
    13201 aggaagctac taaggcagga gctcatgttg agtatatttt agaatcagaa gaagctaagg
    13261 aggcaagact taaattttct acaaaggatc aattattatc agggaattta ataatggaaa
    13321 ctatgcttga aaaggcagat gtttggttaa gtgcatgggg aactagaaat actagagcct
    13381 ttagcaatat agattcagaa aaaataaaag atagcagagc tggagaaaaa ggatggagaa
    13441 aattctattc aggaagaatg ggagatggct ctttaagatg gtgtggaact caatttccta
    13501 catatgcaga tgcacaggaa gcttcaatga gttttagtga atatgaagat tttgtttata
    13561 gagcaggact tttagaccat gaagatcctg tggcagaatg gaatagagta agcaaagaac
    13621 aagaaagatg ggttaaatat ttagatacta aaaaagaact tcatatttta gcagaaggaa
    13681 ctgatattaa ggtctcagta gagggaagaa agtggataaa ttgtgatggt agagtaaact
    13741 tcccagatgg tgaaatattt acatcaccag ttgaaaataa gataaatgga catataactt
    13801 tttcattccc aggtatttat gcaggaaagg aaatagaggg gatagaactt gaagttaaag
    13861 atggtaaagt tgtttcatat aaagctaaaa aaggagaaga tttattaaag gctttattag
    13921 aaactgatga aggagcaagc tattttggag aagtagctat aggtacaaac tatggaatta
    13981 agaagtttac tagaaatatg ctatttgatg agaaaatagg aggaacagtt catatggcta
    14041 taggagattc tatgccagag gctggtggta aaaatagatc atcacttcat tgggacatgc
    14101 tttgtgacat gagaaatggt ggaagaatat atgcagatgg agaacttttc tatgaaaatg
    14161 gagagtttaa aaaagaaata ttagaaaaat ataatcttta atattatata taaattatag
    14221 aataataatt aaattagggg atgaagagac tcatctccta atttaattct agttgaattt
    14281 taaaataatg ggagtataag aattatgagt aatatatatg agtttaaaaa agaagactta
    14341 aatgaatgtg ctgagctttt tgttaaaacc tttagtaagg aaccatggaa tgaaccttgg
    14401 gattttgaaa atgctaaaaa aagacttaat gatgtggttt taactccagg atttaggggt
    14461 gctgttttaa gaaatgatga aaaaatagaa ggcgttatct taggaaattt agagcaatgg
    14521 tatgatggag agcatttttg tgttaaggaa ttctttgtgg attcaagttc acaggggaaa
    14581 ggaacaggaa agaaactttt aaatgcctta gaggatatac ttatggaaaa ggaagtggga
    14641 gtaattcact tttggactat gaaaggaagc acagctgaag ccttttataa taaaagaggg
    14701 tatgaaattc caaaggaatt aataatgatg agaaaaaagt taaaatagta aatttttaaa
    14761 taatgaaggt ctagttgaca attaaaatta ttttaataca cctagaaatt aaaaaattta
    14821 aaataatact atatgttaaa ttttaatata gaagatataa aagagcatgt aataagttta
    14881 aaacattaat acatgctctt tttactattt aatttaaatt ctatatatta ttaaactgta
    14941 agagaaccat ttttctctaa aacttcagct tctttatttt catctttagt atcaatacca
    15001 tcatttttgt attcataaat tacgcttatt agtttaccac ttatacatac tgctattatt
    15061 gagaagaata tttgcttaaa gtctaaataa actaaagaca ttaataaaac aatggcatct
    15121 gttaacatgt agacatgttg taccttaaga ggagtaaatt tattacctaa taaggctatt
    15181 acatcatctc caccagaagc accaccacca cgtaatacta ttccagcgcc aacaccaact
    15241 ccaatacctg ctaaaacact agcaactagc atattattag taaagttagg tgttaaaaat
    15301 cctatgcttt cccagaatct ataggatact gagaagcaaa aagttgcaaa tattgaacat
    15361 gctaagaatt ttttgccaaa gaatttagaa cctattgcaa ataatgaaaa atcaattatt
    15421 aagtttgtta tagatggaga tatatcaaaa acatttttta ctaaaagtaa taagcctaag
    15481 actccacctt ctgttacatt attttgatag ttaaagttat agctaccaaa ggctaagatg
    15541 cttgaaccta gtgctattaa taaaacactt ctcaagatct tctttttcga ttccaaaata
    15601 tcactctcct aaaattaagg taatttagct ataaagaaaa tagcagacct catatgtcta
    15661 ctatatagaa ataaaaaatt atgaatttta aattatattt tatagtaaat tactgtatat
    15721 tttaattata atcttaataa atattaagag caaactatat aaaataagag taatgaggat
    15781 taaaggggca attctttaat agaaataaat agcctctgtg gtcatctgtt tttctttaaa
    15841 gtgttcattt ttacaaggtt ttccgaaagg ttaaaatatt gttgttaata gtaagaaaac
    15901 ttgaaatgaa taaaaggtta atatatataa atttattttt atttgaaata ctataaatga
    15961 aagcaaatac atagatggtt cttaaattta aaagtgatta ttttacataa aatatatctt
    16021 aatttttaaa tttaagaata aaacatatga gaaaggatgt tatagttatg aaagtaattg
    16081 ttagaaaaga gttagaacaa gatcataaaa cagtagagct tttagtagaa gaagccttta
    16141 aagatgctga gtacagtgat cacatggaac atgttttagt tagtaaactt agaaatggag
    16201 aaaactttat tgatggttta tctttagttg ctgaaagaga aggagaaata gtaggccaca
    16261 tattattaac taaagctaat atagaaaatg aaacagaaaa atatgaaact ttagctttag
    16321 caccagtttc agtgttacct aaatatcaag gtatcggaat tggagcagag cttataagag
    16381 aaggaatgag aatggctaag gaacttggat atgattcaat aatagtttta ggacatgata
    16441 aatactatcc taagtttgga tttaaaaaag ctggggattt tggaataaaa gctccttttg
    16501 aagtgccaga agaaaacttt atggcaatag aattaaatga agaagcttta aaaaatataa
    16561 gtggtgttgt aagatatgcc aatgaatttt tagcagataa taataagtaa taactttgat
    16621 atatttattt aaatattaat taaatattga gactatattt gtattaattt aaggtaaaat
    16681 aataaggtgg ttgtataaaa aaattataag tactataatt ttttatcaat caccttgtta
    16741 ttttttagat taaagtttta aacttttgtt tttttatata gatataggag aacacataga
    16801 tatacaatta gagtttaaat atttatataa taaatattta aaatagaatc tatttataat
    16861 atttaaaaag tatttttatt taaataattt attccaaagt aaacatatat tattgatact
    16921 aaaaagctta agggaagagt aatgagcata tttaagctta ataaatttag taatgctaat
    16981 aatactaagc atggagttat tccataagta cttagtttta gtatttccaa aaatcctaca
    17041 gctctatttc ttatactaaa tataatttta gttattatag ctaatattaa ggttataatg
    17101 tatgagaaag caataaatat aataaccatt aaggataaat atgtatttcc aaaggttctc
    17161 tctaaactat ttatagaatt tttcaagtca ttatcattta aagatagatt catatttgaa
    17221 taattagctt cagtaatttt attattgtca tatttaatta ttaaagattc attaccaaag
    17281 gcaattcctg attcataatc ctttaataag tcttttgcag gttccttatc atttagtaca
    17341 aaggtccagt tttcttcttg aaaaactaca aagttagaat cttttaggtc taaaactcca
    17401 tccttaattt caaagggagg aattttgttt tcaaaggcag attttctaga aattaaaaat
    17461 ctattaagtt cagtgtaatt tctactaaaa gctaaaagtc ctaataaaag agataaaata
    17521 agtaaataaa agaaagtgct tattccagaa gttttaataa gttctttata tcttttagga
    17581 gtaaataaac ttatataaaa tcttttaaat aggttcatat aatcaccctt tctatttttt
    17641 attagtttgt tcattaaata gaaatttatt aatactgtta acttttgttt aattaatttg
    17701 aaaacgattt taaaacatag tgtcgtatga tatcataaaa atgtattaaa tggaaaataa
    17761 atgataaaaa gacttttaaa ggatttgata caattaaata ttgaatgaaa tattggggga
    17821 tttttatgat taaaagggat tttagaaaaa agttaactat gtgtttagca tctgttatgg
    17881 ctttaaacct aggtataagt gtatcggtaa aagctgatga gggattaaaa aatgagatta
    17941 agaataatga tattactaaa gatataagca tatatccaac tcctaaggat atgactgtag
    18001 gaaatagtga atttacctta gaagattcag ttaatatagt tggaggagat ttagcagata
    18061 catatgcctt tgaacttttg aaaaatatgt taactgaatt tggcataaaa attaatgaat
    18121 cagaaattga gggggctact actatttata taggagagag cgatgacaat attgaagaga
    18181 tggaaaatac attagaagaa atgaatgtag acacatcttc aataagcaaa gatgagggct
    18241 atgtattagt aactgatgaa aatatagagg gaaataaaat agttataaga ggaaatgatg
    18301 aaacaggaac tttttatggg gtaaaaagtt taaagcaatt aattaaaaag gaagataata
    18361 aggttatctt agatgaagtt gttataaaag atgagccgag ttttaaaatg agatctgtag
    18421 ttgaaggttt ctatggaaca ccatggtctc aagaggaaag attggatcaa ataaaaatgt
    18481 atggagaata taaaatgaat gcttatatat acgcgccaaa gtcagatcca tatcatagag
    18541 aaaagtggag agagccgtat ccagcttctg aattagatag aatgaaagag cttataaaaa
    18601 cagctaatga aaataaggtt gattttgttt ttgcaatatc accaggttta gatataaagt
    18661 ttgagggaga agagggagaa gcagatttta aagctttaat aaataaagcg gagactctat
    18721 atgatatggg agttagaagc tttgcaattc tttgggacga tatagaaaat cgtagtggag
    18781 ttcaacaagc agaagtttta aatagattta ataaagaatt tataaaaaat aaagaagggg
    18841 ttaagccatt aataactgtt ccagtagaat attggggaag ctcaatgttt aatggggaag
    18901 aggtaaagac ttatactaaa gagtttgcag aaactttaga taaggatatt gaagtaatgt
    18961 ggacaggaaa tgatgttatt cctccaaatg gggttagctt agaggatgca aagaaggttt
    19021 caaatgtata taatagaaaa atgatgttat ggtggaatta tcctgttaat gattataaag
    19081 aggataaaat ggctttagga ccaatatatg atttagatag aaatttagat gaggaagtgt
    19141 caggatttat agttaatcct atgagatttt ctgatgcatc aaagatttcc acattaacag
    19201 gagctgatta tggatggaat tcagtttcat atgaagcaga aaaatcatgg gacaaagcta
    19261 ttgaaattat tgcaggagaa atgaaagaag aatttaagat ttttgctaat cattcaacta
    19321 gattagatac aggaagacca gactcaccag agattaaaaa tactatagat agcttatggg
    19381 aaaagtggga ggcaggttct gatattagtt tagaattaag taatttacaa aatgagtttt
    19441 ctaaaatggt tcaggtacca aataaattaa gaagtaattt agaaaataaa gctttattaa
    19501 atcaattaga ttcacactta tcaaagtttg agatttatgg aaatgctgga cttaaatcca
    19561 tagaaatatt acaagacata ttaaataagg atatgtcaga gttctggagt gataattttg
    19621 aaggaataaa acttcttaga aatttagatg gaataaaagc tacaattgct aacaatgtgg
    19681 tggatccatt tataagaaaa gtccatgaag ttggaaatga atatttcaat aaggaaacta
    19741 ctgttttaga ggacaaggaa tattcttata catcaattgg aaatattgct gaccataaat
    19801 attcagaatg gtttatttct gaagaaacgc acgttccaaa ttatatgttt gatgataaag
    19861 ataatacagg cttttggtca gcagcaccag tacaaaagga tgagttcata ggatttgatt
    19921 taggtgaagc tgaagagatt aaagacatat atcttcttat gggaagaaat aataatgata
    19981 cagatgtttt aacaaatgga gtaatagagt attcattaga tggagaaaat tggacagagt
    20041 tagaagttaa taagggagag agagaattac tagttgaaac aggaataaaa gctagattta
    20101 taagatatag agctctttca gactctgaaa ataagttata tgttagagat tttaaagtta
    20161 atactggaaa gagtaaggaa aaattaatag gaaatgtaga agttaaagac tcaaatatta
    20221 ataagtcttc agagggagaa aatgaaatta tatctgcaag taatataaat aagatttcct
    20281 ttgaagaggg agattctata ggacttgcta taaatgatgt taaaaatgta attggatttg
    20341 aagtcttagg agatgtagaa agtaataaat ttgtattaga aagttccttt gataatatag
    20401 agtgggaaga agtttgcaat ggaactagct ttaaagatga gactccttta gtaggaaagt
    20461 tctttagaat aagagcgaag gaaaaaactg aagctaatat taatgaaatt aagatttcat
    20521 tagaaggata ttctaaggga atagtttcaa caaatagaag aata
//
