LOCUS       NZ_CP079946            10825 bp    DNA     circular CON 18-AUG-2024
DEFINITION  Corallococcus sp. EGB chromosome, complete genome.
ACCESSION   NZ_CP079946
VERSION     NZ_CP079946.1
KEYWORDS    RefSeq.
SOURCE      Corallococcus sp. EGB
  ORGANISM  Corallococcus sp. EGB
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Corallococcus.
REFERENCE   1  (bases 1 to 9431171)
  AUTHORS   Zhao,Y. and Cui,Z.
  TITLE     Whole-genome sequencing of Corallococcus sp. strain EGB reveals the
            genetic determinants linking taxonomy and predatory behavior
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9431171)
  AUTHORS   Zhao,Y. and Cui,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2021) College of Life Science, Nanjing
            Agricultural University, No.1 Weigang Road, Nanjing, Jiangsu 210095,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. MAY-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 113.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019968905.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 04:02:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,752
            CDSs (total)                      :: 7,680
            Genes (coding)                    :: 7,614
            CDSs (with protein)               :: 7,614
            Genes (RNA)                       :: 72
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 12 of 66
            Pseudo Genes (incomplete)         :: 62 of 66
            Pseudo Genes (internal stop)      :: 2 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:46:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7772083
            Orig. end                         :: 7782908
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079946.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from College of Life Science,
            Nanjing Agricultural University, China.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10825
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="31"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10825
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10825
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5825](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5825)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(238..252)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.1"
                     /tool="antismash"
     gene            complement(696..3392)
                     /locus_tag="KYK13_RS31545"
     CDS             complement(696..3392)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PPDK_N"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399294.1"
                     /locus_tag="KYK13_RS31545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="WP_223637344.1"
                     /sec_met_domain="PPDK_N (E-value: 3.4e-51, bitscore: 173.3,
                     seeds: 105, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSTLPLVSMTEASDPQQVGQKFARQQHLARHGFPVPEFFCLTTT
                     MFQEVARPLQGAIDRLTTAVDRTSHQDIRRVAEEIEHLFLQVPLGAEREASILEAFDRT
                     FSVGGVVAVRASVVGRALEESEDSETDPFAGVSSTFLYVRRDALLDRIRRCWASGYTPE
                     GLIYRLAQGRDVRGLMVAVGVQRMVPGRRSFVAFTCDPKTTERRTLVVAGHGIGEGVVQ
                     EKVGVDHYFIHPETGHIDRQLAHKADMLCENPEPGGELVRRPVPEPLRDAPCLTDEELK
                     RLARMARDIERSFGKPQDIEGTFTEGGNLHVLQARPIAFDFRKIRVWGCGNVSESFPGV
                     TTPLTYSLASYFYEVIFYDLLRRMKGADARALHDQTPAFQNMLGFIDGRVYHSLSNLMH
                     VNGINPFIAATSMRDLEDVLQRDASLVIRLPGHRESFPSTASYAYALGRSVLGALTVTA
                     TFQRDFEAFGAWWKRTLAGLRALSVEKEDPLVLIHHFRRVWTEVGNWWGLTLINHWYIN
                     TCFGLARRLLLKWTGEDPSALLVGLMCGAKPDAGTHALLSAVALAEQVRADPALAKRFD
                     EGEAEALWKSIDAGDVPPAFARAFREHLREFGDRGVQELNVERPNLRDTPWDLVRVVQS
                     YARSSVTREELRASDRARRTEAEAQLATLLQGHPLRKLAIEQLLEKLRTVIGLREDSRF
                     WRGQLFGFSKQVFAALGQRMSERGVLDAPLDVHYLTMREVLDHFEGRSVTRNLGELARL
                     RRREQQDNQERQPPRDFTTVGSVADGIPRAVEAASDDASLFKGIGSSAGIVEGNARIVL
                     DPGAVGSLSKDDILIAKETDPGWLFLMLASGGIVVERGSMLSHTAIAGRKFGIPTVVGV
                     ANATTRIPDGARVRVDGSTGTVTLVAA"
     gene            complement(3407..4369)
                     /locus_tag="KYK13_RS31550"
     CDS             complement(3407..4369)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399295.1"
                     /locus_tag="KYK13_RS31550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_223637346.1"
                     /transl_table=11
                     /translation="MGLVGGFGVVLTGATGMVGQAFLRQRGAHPVHALVRSPDGRDWGD
                     GVHVVRGDLHGIPDALFPPYPYVVLHLATKQRDPDGTGFERTNVDGTRRLLSRLTGDCR
                     GVLFGSSMSVYGSGPHEGVREDHPLRPVTALARSRVAAEEVITEHAVRTGKHAALLRPR
                     FLLGRGDRFVLPAMVKLLKRGVQLGGDGVGGSVIDVDDLGRILWGLARRMVEAPRPEQQ
                     AFNVAYSRPLLRGEFLGTVARGLGLPPPLVKVRAPGWLPSVLRELPSRRSRAMAERLEL
                     LIHSQCLDVSRLAAVLGPEDLLARSPVDALRSALNDVRS"
     gene            complement(4357..5004)
                     /locus_tag="KYK13_RS31555"
     CDS             complement(4357..5004)
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399296.1"
                     /locus_tag="KYK13_RS31555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="WP_223637348.1"
                     /transl_table=11
                     /translation="MKGLHRWMPTLLLVTGVGLLIPLGIRQVLRTPEDSRPIAGLMLGL
                     YVTWMLVESRVTLRETRKPTAERDSATLELYAIARLTTILLAVALPALELGPVPRAVGL
                     FCFIAGVSLRLMAIRTLGKAYSHRVRLPDASLLVTEGVYRRLRHPAYTGMLLAHVGYLL
                     VFPAVVGIAVFALLFVPAVLLRIRQEERLLLGSFPGYAEYAASRKRLVPWVW"
     gene            complement(5001..5825)
                     /locus_tag="KYK13_RS31560"
     CDS             complement(5001..5825)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399297.1"
                     /locus_tag="KYK13_RS31560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_223637350.1"
                     /sec_met_domain="AfsA (E-value: 1.5e-21, bitscore: 75.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDSDSSHRRRGVRVASPEAVPFLLVPEPLLAEVEHPCRIPVKDVE
                     EAEFLAAALQEHCGLYLGTKAADFPALAEWADATPVGVRVGDPWRKEPPENVSELLARI
                     PVRNPVTEGRQSFSEAEQARPLERIDPVLVHKRDPANVLLANACRVGALQQFNAFTESP
                     EFIFDHPSGHVQGMLLTELARQASIAAIHGAGLPLDWSLIMMRLSLEFRRFVRSDVPVV
                     IRAFVSFRLPEWAGPVRSVGKRSRMWIAVQGWQEDFCCFSGLIGALSAKEAS"
     misc_feature    5813..5828
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.88"
                     /tool="antismash"
     misc_feature    5834..5848
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.32"
                     /tool="antismash"
     misc_feature    complement(5857..5877)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.17"
                     /tool="antismash"
     misc_feature    5874..5888
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.6"
                     /tool="antismash"
     gene            6331..8853
                     /locus_tag="KYK13_RS31565"
     CDS             6331..8853
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /locus_tag="KYK13_RS31565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ELWxxDGT repeat protein"
                     /protein_id="WP_223637352.1"
                     /transl_table=11
                     /translation="MGTISRRAWLSALALVVGSGPAFAKSSSDGLDPMSVRRVMDVTVE
                     PRFELDLLRSGTQPTAELNGRLYFLATPSGATLQDLWVTDGTTAGTRSVQEELLPPGAH
                     IEPGSLRATPHRLFFTATSPETGRELWTSDGTREGTRITRELNSGQLDGVAAQGPLFVF
                     GDSVLFTPAGMGETEFDLWKSDGTTEGTVPVKDIASGATQANADGFVRVGNTVFFSAKN
                     ELGTELWRTDGTEAGTTLVRDIATGAGDSSPRDLTAVGNTLYFTADDGQHGRAVWKSDG
                     TEAGTVRIKDFNPAATGLVPTQLAAVGDTLVFVATSTEAASQLWRTDGTDAGTQQLTAL
                     PSAMLNRGSGQLLVPAGHRAFLVARGAMLGDSVWVTDGTAAGTRRLAHPTAYGHESVRG
                     FAPWEDGLAFIVTTETGASELWRSDGTDAGTSRLTRLPSGHPLAPPAGLVPFQGALLTS
                     AGDAWTSRALWRMERDDASPTFVCPARLDVPSNGPSGTVRVDFKPELRTGPDRAWIRSS
                     FEDGQQIPLHQTLSVTYQAGDDQGRIAYCTTRMTSLDLTPPAIANCPTGLTVSAQSPRG
                     ATFYYPKLGIADETTQDVLVVYSPTNGSLLPVGTTRMNVTAKDASGNESTCAFPVTVRN
                     TQAPSKACKVNEVRVEAEGPQGAHAYWPDLTPVDSMSNKVTVTSTHASGDLFPLGVTPV
                     QVVSTDEAGNSSTCEIQVRVRDSRPPTLTCPEDQTLTTAVMSGTRAEYPEATAEDAVSA
                     TQITYSPVVGTPLAPGLHSVQVTAMDEAGNAALCDFHVTVQYDPTTDMRRGLGCSVGSG
                     PSAAAGWGAVLALAWTLSRRRKDGTPRA"
     gene            8906..9448
                     /locus_tag="KYK13_RS31570"
     CDS             8906..9448
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399302.1"
                     /locus_tag="KYK13_RS31570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF350 domain-containing protein"
                     /protein_id="WP_223637354.1"
                     /transl_table=11
                     /translation="MDLTLFAVGLVKVVLGGLVAALGIGLSMRGLSRLLDSHPVEELRQ
                     GNVAAGLVHAMCLVSLGLLVQHALKATSDAVDLAVQNPPVSFVAVLQLLGLALVHVALS
                     LAVGVAVLALGVRLFDKMTPGIEELAEVRKGNIAAALLLCAFLLVIALLTAPGLQAALN
                     GLIPFPQLPTGMLRAPA"
     gene            9457..10329
                     /locus_tag="KYK13_RS31575"
     CDS             9457..10329
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399303.1"
                     /locus_tag="KYK13_RS31575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_223637356.1"
                     /transl_table=11
                     /translation="MAARSWKTLLSLVLAGALMALASWQLVVTRKAGRLAEAPKPAVEV
                     PPASPQEALKDLGIVLVPEDTPPERAKSYDWRVEGMEPARQQLAYGLGEAVERGLEQAH
                     RDYSVRLHYRAMGPERFTYVAPPGCGTDMRCIYAELMRSNAEPVRALGERFAASIRERD
                     LDAAQATELILGFVRRIRYELPGDEPFGIVPPGLVPAQDRGDCDSKAVLALMLLRQVGV
                     DAVMLYSDALAHAAIGVGLPGTGTRIPFGGRGYQYAELTAEGWPLGMIPPQYDKPQLWR
                     VLPLPDAPG"
ORIGIN
        1 caccatgaag gcaatcaccg cgctggcggt ggaccccact tcgtcggagt agagcttctc
       61 ccctggcttc gtcgtccccg gccacgcggt cttccgcatc acctcccagt gcaggtacag
      121 gaagaccatt cccaggatgg ccagcagccc ttggggattc agtggccttc cggtgcgctc
      181 cgcgtgcgcc acccagacgt agaggccgtg gcccacgtgg agctggatgg cgattccaat
      241 catcaggaac atgctgcgct ggtacggagc ccaattcttc tccagccacc agagcaccag
      301 ggcgtgcacc atgatacccg acaggatggc ggccctcacc caggacaccg ccaggttcgt
      361 cccgagcaac acggccgcgt tgagccccag cgccacgtac aggtccccga agctgacgtc
      421 ccccttcgcc agcccccggt ccgggttgtg gaccaagtcg tattcgtagt cgcgcagctc
      481 atccacgatg cgcatgaagt agcccacgcc gaagatgctc agcgcgacga gcagcaggtc
      541 cgcggagaac gccagtcccc cgccgccgtc gagcactcca aacatcccgt ccaggctcac
      601 gcaccagagc gtgatgacca acaggtagaa cagcggcggg taacgctgca cggagaagcg
      661 cagcgtcgcc ctcacccgct ccatgacggt cctgctcatg ccgccaccag cgtgaccgtg
      721 cccgtggaac catccacccg gaccctggct ccatccggga tgcgcgtcgt cgcgttggcc
      781 acgcccacca ccgtggggat gccgaatttc cggcccgcga tggctgtatg cgacagcatg
      841 ctgccgcgct ccaccacgat gcctcccgac gccagcatca agaacagcca gcccggatcc
      901 gtctccttcg cgatcaggat gtcgtccttg ctcagcgagc ccaccgcgcc cgggtccagc
      961 acgatgcgcg cgttgccctc tacgatgccc gcgctcgaac cgattccctt gaacagcgag
     1021 gcgtcatcac tggccgcctc caccgccctg ggaatgccat ccgccacgct gcccaccgtg
     1081 gtgaagtctc gcggcggctg gcgctcctgg ttgtcctgtt gctcccgccg ccgcagcctc
     1141 gcgagctctc ccaggttcct cgtgacactc cggccctcga agtggtccag cacctcgcgc
     1201 atcgtgaggt aatgcacgtc cagcggcgca tcgagcacgc cccgctccga catccgttgt
     1261 cccagcgccg cgaacacctg cttgctgaag ccgaagagct ggccccgcca gaaccggctg
     1321 tcctcgcgca gaccaatcac cgtccgcaac ttctccagga gctgctcgat ggccagcttc
     1381 cgcagcggat gcccctggag gagcgtggcc aactgcgcct ccgcctccgt gcgccttgcc
     1441 ctgtccgacg ccctcagctc ctcacgtgtc acggacgagc gagcgtagga ctggacgact
     1501 cgcaccaggt cccacggcgt gtcccgcagg ttcgggcgct ccacgttcaa ctcctgcacg
     1561 ccccggtccc cgaactcgcg caggtgctcc cggaaggcac gggcgaacgc gggcggcaca
     1621 tccccggcgt cgatgctctt ccagagcgcc tccgcctccc cttcgtcgaa gcgcttcgcc
     1681 agcgccggat ccgctcgcac ctgctccgcg agcgccaccg cggagagcaa cgcgtgggtg
     1741 cccgcgtccg gcttcgcccc gcacatcaag cccaccagca gcgcgctggg gtcctcgccg
     1801 gtccacttga gcaacagcct gcgcgccagt ccgaagcacg tgttgatgta ccagtggttg
     1861 atcagcgtca gcccccacca gttccccacc tccgtccaga cgcgccggaa gtgatggatg
     1921 aggaccagcg ggtcctcctt ctccacggac aacgcccgca accccgcgag cgtccgcttc
     1981 caccacgccc cgaacgcttc gaagtcccgc tggaacgtcg cggtgaccgt cagcgccccc
     2041 agcaccgagc gccccaacgc atacgcgtag ctcgccgtac tgggaaagct ctcacggtgc
     2101 cccggcagcc ggatgaccag cgaggcgtcc cgctggagca cgtcctccag gtcccgcatg
     2161 gaggtggccg cgatgaatgg gttgatgcca ttgacgtgca tcaggttcga cagcgagtgg
     2221 tagacgcgcc cgtcgatgaa ccccagcatg ttctggaacg cgggcgtctg gtcatgcagg
     2281 gccctcgcgt ccgcgccctt catgcggcgc agcaggtcgt agaagatgac ctcgtagaag
     2341 tagctggcca gcgagtacgt cagcggcgtc gtgacgcccg ggaagctctc cgagacgttg
     2401 ccgcaacccc acacacggat cttccggaag tcaaaggcga tgggcctcgc ctgcaacacg
     2461 tggagattgc cgccctccgt gaacgtgccc tcgatgtcct gcggcttgcc gaagctgcgc
     2521 tcgatgtccc tcgccattcg ggccaggcgc ttcagctcct catccgtgag acacggcgcg
     2581 tcccgcaaag gctccggcac ggggcggcgg accagctcgc ccccaggctc ggggttctcg
     2641 cagagcatgt ccgccttgtg ggcgagttgc cggtcgatgt ggcccgtctc cgggtggatg
     2701 aagtagtggt ccacgcccac cttctcctgg accacgccct ccccgatgcc gtgccccgcg
     2761 acgaccagcg tgcggcgctc cgtcgtcttc gggtcgcacg tgaaggccac gaacgagcgg
     2821 cggcccggaa ccatccgctg cacgcccacc gccaccatca gtccccggac gtcccggccc
     2881 tgcgcgagcc ggtagatgag cccttccggc gtgtaaccgg acgcccagca gcggcggatg
     2941 cggtccagca gcgcgtccct ccgcacgtag aggaacgtgg agctcactcc cgcgaatgga
     3001 tccgtctccg aatcctcgct ctcctccagc gcccgcccca ccaccgaggc ccgcacggcc
     3061 accaccccgc ctacgctgaa cgtccggtcg aaggcctcca ggatggacgc ttcgcgctcc
     3121 gcccccaggg gcacctggag gaagaggtgc tcaatctcct ccgcgacgcg gcggatgtcc
     3181 tgatgggacg tccggtccac cgcggtggtg agccggtcga tggcaccctg aaggggccgc
     3241 gccacctcct ggaacatcgt ggtggtgagg cagaagaact cggggaccgg gaagccgtgc
     3301 cgcgccaggt gctgctgacg cgcgaacttc tggcccacct gctgcggatc gctggcctcg
     3361 gtcatggaga cgagcggaag agtcgatgac atgacaggga attccttcag gaacgaacgt
     3421 cattgagcgc gcttcgcagg gcatccaccg gagacctcgc caataggtcc tccggcccaa
     3481 gcaccgccgc gagccgcgag acatccagac actgtgaatg gatcagcaat tccagacgct
     3541 ccgccatcgc gcgcgacctc cggctgggga gctccctcaa gacgctcggg agccaaccgg
     3601 gagcgcggac cttcaccagg ggcgggggca gccccagtcc ccgggccacc gtgcccagga
     3661 actccccgcg cagcagcggg cgcgagtacg cgacgttgaa cgcctgctgt tcgggacgcg
     3721 gcgcctccac catccggcgc gcgagccccc agaggatgcg ccccaggtca tccacatcga
     3781 tgaccgagcc ccccaccccg tcgcctccca gctgcacgcc ccgcttcagc agcttcacca
     3841 tcgcgggaag gacgaaccga tccccgcgcc cgagcaggaa cctcggccgg agcagcgccg
     3901 cgtgctttcc cgtccgcacc gcgtgctccg tgatgacctc ctccgccgcc acgcgcgaac
     3961 gggccagcgc cgtgacgggc ctgagcggat ggtcctcccg gacgccctcg tgcgggcccg
     4021 agccatagac agacatcgag ctgccgaaga ggacgccacg gcagtccccg gtgagccgtg
     4081 acagcagccg ccgcgtcccg tccacgttgg ttcgttcgaa gcccgtgccg tccgggtccc
     4141 gctgcttcgt cgcgaggtgc aggacgacat acgggtacgg cgggaacaac gcgtctggaa
     4201 taccgtgcag gtctccccgc acgacatgca ccccgtcgcc ccagtcacgg ccgtcgggcg
     4261 agcgcaccaa cgcgtgcacg ggatgcgctc cgcgctggcg caggaacgcc tgtcccacca
     4321 tgcccgtcgc gcccgtgagc acgacgccga agccgcctac cagacccatg ggaccagccg
     4381 cttgcggctc gccgcgtact cggcgtagcc cgggaaggag cccaggagca gccgctcctc
     4441 ctgccggatg cgcaggagca ccgccggaac gaagagcagc gcgaacaccg cgatgcccac
     4501 gaccgcgggg aacacgagca ggtagcccac gtgcgccagc aacatgccgg tgtacgccgg
     4561 gtgccggagc ctccggtaca ccccttccgt cacgagcagg ctcgcgtccg gcagccgcac
     4621 ccggtgcgaa taggccttcc ccaacgtgcg gatggccatg agccgcagcg acaccccggc
     4681 gatgaaacag aacaacccca ccgcgcgcgg cacgggcccc agctccagcg cggggagcgc
     4741 gaccgcgagc aggatggtcg tgagccgcgc aatggcatac agctccagcg tggcgctgtc
     4801 ccgctccgcg gtgggcttgc gcgtctcgcg cagcgtcacc cggctctcca ccagcatcca
     4861 cgtcacgtac aggcccaaca tcagccccgc gatgggccgg ctgtcctccg gcgtgcgcag
     4921 gacctgccgg atgccgagcg gaatcagcag ccccacgccc gtcaccagca acagcgtggg
     4981 catccaccgg tgcaggccct tcatgacgcc tccttcgcgc tcagcgcgcc aatgagcccg
     5041 gagaagcagc agaagtcctc ctgccagccc tgcacggcga tccacatgcg gctgcgcttc
     5101 ccgacgctgc gcaccggccc ggcccactcc ggcagccgga agctgacgaa ggcgcggatg
     5161 accaccggca cgtcgctgcg cacgaagcgc cggaactcca gcgacaggcg catcatgatg
     5221 agcgaccagt ccagcggcag ccccgcgccg tggatggccg cgatgctcgc ctgccgcgcc
     5281 agctccgtga gcagcatgcc ctgcacgtgc ccggagggat ggtcgaagat gaactccgga
     5341 gactcggtga acgcgttgaa ctgctggagc gcccccaccc ggcatgcgtt ggcgagcagc
     5401 acgttcgccg ggtcccgctt gtgcacgagc accggatcga tgcgctccag cggccgggcc
     5461 tgctccgcct cactgaagga ctgccggccc tccgtgacgg ggttgcgcac ggggatgcgg
     5521 gccaggagct cggagacgtt ctccggcggc tccttccgcc atgggtcgcc cacgcgcacg
     5581 cccacgggcg tcgcgtccgc ccactccgcc agcgcgggga agtccgcggc cttcgtgccc
     5641 aggtagaggc cacagtgctc ctggagcgcg gcggccagga actccgcctc ctccacgtcc
     5701 ttcaccggaa tgcgacaggg gtgctcgacc tccgccagca acggctccgg tacaaggagg
     5761 aaggggacgg cctccggcga cgccacccgc acgcctcgcc ggcggtggct ggaatcagaa
     5821 tccatgtctg cctttctcca tctgaggggg agaggcagtc taggaattgc gatgtctcaa
     5881 cacgagaacc ggggaaggcc agcgctcccg gcccggtgga gcaaacccgg agggccttgc
     5941 gggcttgttg catgatccgt catcagacgt ctcaaaaccg tgtcttggat tgagacggcc
     6001 ggtgcggccc ggggctcact gtccctcggg agagtcccgc tcggggcgga cccgcgcctc
     6061 ccgccgcgct ccccaccatc caggtagatg gtgcccatcg caggggacgc accggagtcc
     6121 tggcaaccca ccgccacccg cacggggtgt gtccacgtgc gcgtcctccc gagcgcagcg
     6181 gccgattatg aacacctgcc gtgggggcga ggaccgtggg acgcaacctg tctgacagtc
     6241 ggacggggcg cgggttgtcg cggggcggat gcgtgatgac ggtgcgcccc cggtacgcgg
     6301 cgttggcgta acgggcgtgg aggggcgggc atggggacga tcagccgtcg tgcgtggctt
     6361 tcggcgttgg cgttggtggt gggctctggc cccgcgttcg cgaagagctc atccgacggc
     6421 ctggatccga tgtcggtgcg ccgggtgatg gacgtcaccg tggagccccg ctttgaactg
     6481 gacctcctcc gctccggcac gcagcccacc gccgagctga acgggcgcct ctacttcctc
     6541 gccactccga gcggcgccac gctccaggac ctctgggtga ccgacggaac gaccgcgggc
     6601 acccgctccg tgcaggagga gctgctgccg ccgggcgctc acatcgaacc gggctcgctc
     6661 cgcgccacgc cgcaccgcct gttcttcacc gcgacgtcgc cggagaccgg acgcgagctg
     6721 tggacgagcg acggcacccg tgaggggacg cgcatcacgc gcgagctcaa ctccggccag
     6781 ctcgacggcg tggccgccca ggggcccctc ttcgtcttcg gggactccgt gctcttcact
     6841 ccggcgggca tgggggagac ggagttcgac ctctggaagt cggacggaac caccgagggt
     6901 accgtgcccg tcaaggacat cgcctcgggc gcaacccaag cgaacgcgga cggcttcgtg
     6961 cgcgtgggga acacggtctt cttctccgcg aagaatgagc tgggcaccga gctgtggcgc
     7021 acggacggca ccgaggcggg caccacgctc gtgcgcgaca tcgcgaccgg cgccggcgac
     7081 tcgtctcccc gggacctcac cgcggtgggc aacacgctct acttcaccgc ggacgacggc
     7141 cagcatggcc gcgcggtctg gaagtccgac ggcacggagg cgggcaccgt gcgcatcaag
     7201 gacttcaatc ccgccgccac gggcctcgtg cccacccagc tggccgccgt gggcgacacg
     7261 ctcgtcttcg tcgcgacgag cacggaggcg gcttcgcagc tgtggcgcac ggacggaacg
     7321 gacgcgggga cccagcaact gacggcgctg cccagcgcga tgctcaaccg cggttcagga
     7381 cagctcctcg tcccggcggg ccaccgcgcc ttcctggtgg cccggggcgc gatgctcggg
     7441 gactcggtgt gggtgacgga cggaaccgcc gcgggcaccc ggcgcctcgc gcaccccacc
     7501 gcgtacgggc acgagtccgt gcgcggcttc gcgccctggg aggacgggct cgccttcatc
     7561 gtgacgacgg agacgggggc gagcgagctg tggcgctcgg atgggaccga cgcgggcacg
     7621 tcccggctga cacggctgcc ttcgggccac ccgctcgcgc cgccggcggg gctggtgcca
     7681 ttccagggtg cgctcctgac gtcggctggc gacgcatgga cgtcgcgagc cctgtggcgg
     7741 atggagcggg atgacgcgtc gccgaccttc gtgtgccctg cccggttgga tgtgccctcc
     7801 aacggtccgt cgggcacggt gcgggtcgac ttcaaacccg agctgcggac cgggccggac
     7861 cgggcgtgga tccgctcctc gttcgaggac ggccagcaga ttccgctcca tcagaccctc
     7921 tccgtcacct accaggccgg ggatgatcag ggtcgcatcg cgtactgcac cacgcggatg
     7981 acctcgctgg acctgacgcc gccggccatc gccaactgcc ccacggggct caccgtgagc
     8041 gcgcagtcgc cccggggcgc caccttctac tatccgaagc tgggcatcgc ggacgagacg
     8101 acccaggacg tgctcgtcgt ctacagcccg accaacggca gcttgctgcc cgtgggcacc
     8161 acccggatga acgtgacggc gaaggacgcg tccggcaacg agagcacctg cgccttcccg
     8221 gtcaccgtcc gaaacaccca ggcccccagc aaggcctgca aggtgaacga ggtccgcgtg
     8281 gaggcggaag gcccccaggg cgcgcacgcg tactggccgg acctcacgcc cgtcgacagc
     8341 atgtccaaca aggtcaccgt caccagcacg cacgcgtccg gagacctgtt cccgctgggc
     8401 gtcacccccg tgcaggtcgt gagcaccgac gaggcgggca atagcagcac ctgcgaaatc
     8461 caggtccggg tgcgcgactc gcgcccgccc acgctcacct gcccggagga ccagacgctc
     8521 accaccgcgg tgatgtccgg cacccgcgcg gagtacccgg aggccaccgc cgaggacgct
     8581 gtctccgcga cgcaaatcac ctacagcccc gtcgtgggca cgccgctcgc gccgggcctg
     8641 cactcggtgc aggtcaccgc catggacgag gccggcaacg cggcgctctg cgacttccac
     8701 gtgaccgtgc agtacgaccc gacgacggac atgcggcggg gcctgggctg ctcggtgggc
     8761 agcggcccct ccgcggcggc gggctggggc gccgtcctcg ccctcgcctg gacgctcagc
     8821 cgccgtcgga aggacggcac gccgcgcgcg tgagtcatgg gccatggcgg cgaggaggac
     8881 ttccgctaca gtcctgaccc gccccatgga cctgaccctc ttcgccgtcg gactcgtgaa
     8941 ggtcgttctc ggtggtctcg tggccgcgct cggcatcggc ttgagcatgc gcggcctgag
     9001 ccgcctgctg gacagccacc ccgtggagga gctgcgccag ggcaacgtcg ccgccgggct
     9061 ggtgcacgcg atgtgcctcg tgtcgctggg cctgctcgtg cagcacgcgc tgaaggccac
     9121 cagcgacgcg gtggacctgg cggtgcagaa cccgcccgtg tccttcgtcg cggtgctcca
     9181 gctgctgggc ctggcgctgg tgcacgtggc gctgtcgctg gcggtgggcg tggcggtgct
     9241 cgccctgggc gtgcgcctgt tcgacaagat gacgccgggc atcgaggagc tggcggaggt
     9301 gcgcaagggc aacatcgccg ccgcgctgct cctgtgcgcc ttcctgctgg tcatcgcgct
     9361 gctcaccgcg ccgggcctgc aggccgcgct caacggactc atccccttcc cgcagctccc
     9421 caccggcatg ctgcgcgcgc ccgcctgaaa gccgccatgg ccgcgcgctc gtggaagacg
     9481 ctgctgtcgc tcgtgctggc gggcgcgctc atggccctcg ccagctggca gctcgtggtg
     9541 acgcgcaagg cgggccggct cgcggaggcc ccgaagccgg ccgtggaggt gcccccggcc
     9601 agtccccagg aagcgctgaa ggacctgggc atcgtgctgg tgccggagga cacgcccccg
     9661 gagcgcgcga agtcctatga ctggcgcgtg gagggcatgg agcccgcacg tcagcagctg
     9721 gcctacggcc tgggcgaggc ggtggagcgc gggctggagc aggcgcaccg cgactacagc
     9781 gtgcggctgc actaccgcgc catgggcccg gagcgcttca cctacgtggc gccgccgggc
     9841 tgcggcacgg acatgcgctg catctacgcg gagctgatgc gcagcaacgc ggagccggtg
     9901 cgcgcgctgg gcgagcgctt cgccgcctcc atccgcgagc gcgacctgga cgcggcgcag
     9961 gccaccgagc tcatcctcgg cttcgtgcgc cgcatccgct acgagctgcc cggagatgaa
    10021 cccttcggca tcgtcccgcc ggggctggtg cccgcgcagg accggggcga ctgcgactcc
    10081 aaggcggtgc tcgcgctgat gctgctgcgc caggtgggcg tggacgcggt gatgctctac
    10141 tcggacgcgc tcgcgcacgc ggccatcggc gtgggcctgc ccggcaccgg cacccgcatc
    10201 cccttcggag ggcgcgggta ccagtacgcg gagctcaccg cggagggctg gccgctgggg
    10261 atgatcccgc cccagtacga caagccgcag ctgtggcggg tgctcccgct gccggacgca
    10321 ccgggctgag cgccactggc gattcacacc gaatccaagt attcctgctc tatccgggat
    10381 gctcgtcaag ggcgggcatc ccctcaccca agcgggggca ggcacatggc ggcttcacgg
    10441 acgtggatga cgttggggct gatggcactg gcaacaggct gtggcgcggc ggaggacacg
    10501 gcggctccga gcgtggtgga gcaggcgctg ggcgcgtgca cgcactcggt gacgacgaac
    10561 acgtatgtcg gcccggacta ctggggcacg ctcgcgttca agaacacggg gacggtggcc
    10621 atcgccaacc cggtcatcgc gctcgacgtg ccctcgggcg tgacgtgtga cgatgatcaa
    10681 cccggctgga cgcacacgca gtccggccgc acgtgcacct tcacgcgcac gtcgtcgctg
    10741 acggtggcgg tcaacgcgtc ctacacgttc aactactcca ccacctccag cacgtccttc
    10801 accgccgcca acgtgaaggt ccagt
//
