LOCUS       NZ_CP079946            11647 bp    DNA     circular CON 18-AUG-2024
DEFINITION  Corallococcus sp. EGB chromosome, complete genome.
ACCESSION   NZ_CP079946
VERSION     NZ_CP079946.1
KEYWORDS    RefSeq.
SOURCE      Corallococcus sp. EGB
  ORGANISM  Corallococcus sp. EGB
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Corallococcus.
REFERENCE   1  (bases 1 to 9431171)
  AUTHORS   Zhao,Y. and Cui,Z.
  TITLE     Whole-genome sequencing of Corallococcus sp. strain EGB reveals the
            genetic determinants linking taxonomy and predatory behavior
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9431171)
  AUTHORS   Zhao,Y. and Cui,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2021) College of Life Science, Nanjing
            Agricultural University, No.1 Weigang Road, Nanjing, Jiangsu 210095,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. MAY-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 113.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019968905.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 04:02:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,752
            CDSs (total)                      :: 7,680
            Genes (coding)                    :: 7,614
            CDSs (with protein)               :: 7,614
            Genes (RNA)                       :: 72
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 12 of 66
            Pseudo Genes (incomplete)         :: 62 of 66
            Pseudo Genes (internal stop)      :: 2 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:46:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4588654
            Orig. end                         :: 4600301
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079946.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from College of Life Science,
            Nanjing Agricultural University, China.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11647
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="15"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11647
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11647
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6647](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6647
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    189..203
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.7"
                     /tool="antismash"
     gene            558..896
                     /locus_tag="KYK13_RS19180"
     CDS             558..896
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KYK13_RS19180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_223646246.1"
                     /transl_table=11
                     /translation="MRTMLDRVHGAQMRGAGRRGLREALAMEGVLITTHLEVWSRALEG
                     VCPDCIAEVAPETDAKLQDPEAMHEAALERWRTSAPELVSDAVESAVDDVLQAAREAAT
                     RNKPPPSE"
     misc_feature    1169..1183
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.09"
                     /tool="antismash"
     gene            1306..2499
                     /locus_tag="KYK13_RS19185"
     CDS             1306..2499
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /locus_tag="KYK13_RS19185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4331 family protein"
                     /protein_id="WP_223646248.1"
                     /transl_table=11
                     /translation="MALSLGAGSASASDHLDTPTVIEDPSADIADLFAWMSPDGQRLNL
                     AMTVVAHRFSDQLQYVFHVSSGKRFGETTADLSIQCRFDAANTAECWAGDVDHVRGDAS
                     RVAGIEGRQKRFRVFAGLRDDPFFNNVKGTRAALDVAKAALQGGAPVDAARCPRFDAAT
                     TRSILEQWRQTQGGPGKNFLAGWSSSALVVSIDRDAVGKAGPYLAVWAGIYKSNPDARK
                     GTSARPKLGAPVDRIGRPLTGNALIGPLEPDDVSDRRKEEYNRAAPADWAGFAPDLQRT
                     LGLYDGLDGTCGNQWLANAGGEPAQRYQRLAKLLADDRLWINSRATVCKQYLSVELAEL
                     STPGKPGTDCGGRTPLEDSNDVFRSLLALGATSGIDDGVKEDDHVHSLSDFPFLAAP"
     gene            2496..3608
                     /locus_tag="KYK13_RS19190"
     CDS             2496..3608
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /locus_tag="KYK13_RS19190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_223646250.1"
                     /transl_table=11
                     /translation="MRPSESWSTWLAVAVMVCGGATAAWGEAPRYVTTTGALAIENLDH
                     RIALQRADAGVGELLLLRARFLSDYDALDRASTLAEERSRTAGDLLERARARSAVHRFA
                     EALTDLDAAGKDDEASSDLRASILVATGHASEAISRLEARVAQRPGYASRSALAGAYAA
                     VGRFTDADRLYSEALADLDTTSPFPYAWIYFARGVMWAEQAGDPARAEPLYARALAHLP
                     EFAMANVHLAELEAARGDVASAMTRLERVVASTHEPEALALLGTLHLRTGAPVRGRSEI
                     TAARERFEALLVRQPLAFADHAARFYLGPGADPERAWVLAQQSLEGRQTASAFTLAIEA
                     ARATGRECEACVLVEASRARGLPVPGDGCP"
     gene            complement(3618..4403)
                     /locus_tag="KYK13_RS19195"
     CDS             complement(3618..4403)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (6-166). E-value: 3.1e-22. Score:
                     71.3. Matches aSDomain:
                     nrpspksdomains_KYK13_RS19195_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396622.1"
                     /locus_tag="KYK13_RS19195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_223646252.1"
                     /sec_met_domain="adh_short (E-value: 3.4e-38, bitscore:
                     130.2, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 6.7e-40, bitscore:
                     136.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLKGKTALITGGNSGIGLATARLFVAEGARVAITGRNRETLDAV
                     VKKLGANVLAVQADATEPSALERAVAATVERFGGLDIVFANAGIAERTPVGRTSHEAFT
                     SVLQTNVTGVFFTVQAAAAHLKPGASVILTSSVHSELGMPGYSAYAASKGALKAMASVL
                     AAELAPQGIRVNVVSPGATNTPIWEKDAPTPESFAKLERRMSATIPLGRIGKPEEIART
                     VLFLASDEASYVNGQDLFVDGGVNGAALGAPLFRAPQSA"
     CDS_motif       complement(3891..3944)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KYK13_RS19195_0003"
                     /evalue="3.30E+01"
                     /label="PKSI-KR_m1"
                     /locus_tag="KYK13_RS19195"
                     /protein_end="171"
                     /protein_start="153"
                     /score="-2.7"
                     /tool="antismash"
                     /translation="GALKAMASVLAAELAPQG"
     aSDomain        complement(3906..4385)
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KYK13_RS19195_PKS_KR.1"
                     /evalue="3.10E-22"
                     /label="KYK13_RS19195_PKS_KR.1"
                     /locus_tag="KYK13_RS19195"
                     /protein_end="166"
                     /protein_start="6"
                     /score="71.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALITGGNSGIGLATARLFVAEGARVAITGRNRETLDAVVKKLGA
                     NVLAVQADATEPSALERAVAATVERFGGLDIVFANAGIAERTPVGRTSHEAFTSVLQTN
                     VTGVFFTVQAAAAHLKPGASVILTSSVHSELGMPGYSAYAASKGALKAMASVLAAE"
     CDS_motif       complement(3951..4010)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KYK13_RS19195_0002"
                     /evalue="2.50E-04"
                     /label="PKSI-KR_m4"
                     /locus_tag="KYK13_RS19195"
                     /protein_end="151"
                     /protein_start="131"
                     /score="13.6"
                     /tool="antismash"
                     /translation="ILTSSVHSELGMPGYSAYAA"
     CDS_motif       complement(4290..4379)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KYK13_RS19195_0001"
                     /evalue="2.80E-08"
                     /label="PKSI-KR_m1"
                     /locus_tag="KYK13_RS19195"
                     /protein_end="38"
                     /protein_start="8"
                     /score="26.4"
                     /tool="antismash"
                     /translation="LITGGNSGIGLATARLFVAEGARVAITGRN"
     misc_feature    4467..4479
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.7"
                     /tool="antismash"
     gene            4490..4966
                     /locus_tag="KYK13_RS19200"
     CDS             4490..4966
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396623.1"
                     /locus_tag="KYK13_RS19200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_223646254.1"
                     /transl_table=11
                     /translation="MSPRKEAAVKRTSMEGVMCPVARSLDVIGDWWSLLIVRDAQYGLR
                     RFGEFQKSLGVAKNILAQRLRHLVDHGILETQPASDGSAWQEYVLTEKGQSLFPILVAL
                     GQWGQEHCFQAGEPRTRVLDKAHGKPVKPLEVRAADGRVLTLKDVRLERPPSQG"
     gene            5001..6647
                     /locus_tag="KYK13_RS19205"
     CDS             5001..6647
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396624.1"
                     /locus_tag="KYK13_RS19205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_223646256.1"
                     /sec_met_domain="DUF692 (E-value: 2e-25, bitscore: 88.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRPASDSWSLPWMGLGLSSNLSASDVPQPYRLLDASPGLFDFVE
                     YSAPIALEEAKAQATLFPEMWRRRSEVPVLFHPVHLNLWGPELEPASALAQLDAHARAV
                     GSPWVGNDVGWWHAGGQPFPGYLYVTPPFNEAGVRDSAAHALHIQAGLSVPLVIENPAV
                     LATRGGMHALDFMAKLHARTGLPLLLDLGHLFSHQLSAGLPLRTGLDGFPLDQVVELHI
                     AGGVVTRRGGRTFYVDDHTQPVREELFELLAEVLPRCPRLRAVTFEGDGHPPEVALASL
                     RRLRALVTGASRPAIDVPASRAPVPALTGDSRPWALFDAGHGVTPAAEDEDPEGTRADR
                     DFRLAVVAEQLDKEWPLTRLLLAATPEGLESFTRSREYRGLFDGLGRSLSHAFASWARK
                     RVMASPSDGVAAALSLEMMLPHAFLQEPPAPAPGQVALAEDVRLGSFPADLTELVFAAR
                     ALRRHLTGRAWACGALEVSGLEALAQVAARPGPGPWRFAIRRKAPGFEVLTLPPEVAEG
                     LRGLADGPLPAESLPGWLLAEGQARGLLRRA"
     gene            6715..7353
                     /gene="udk"
                     /locus_tag="KYK13_RS19210"
     CDS             6715..7353
                     /EC_number="2.7.1.48"
                     /GO_function="GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="udk"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396625.1"
                     /locus_tag="KYK13_RS19210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="uridine kinase"
                     /protein_id="WP_223646258.1"
                     /transl_table=11
                     /translation="MSSPLVVGIAGGTASGKTTVARKVREALADCRVAFIDQDSYYRDL
                     KDMSLEERREVNFDHPDAFDTELLVSHLRALKQGHAIQKPVYDFVTSGRQPHTHRVDPG
                     DIILIEGILVLHMKEVRDEMDVKIYVDADDDLRILRRLTRDIKDRGRDFDHVVGQYLRH
                     VRPMHMGFVEPSKHHADIIIPHGGNNDIAIGMLVGALRARLATQHQAQR"
     gene            complement(7350..8402)
                     /locus_tag="KYK13_RS19215"
     CDS             complement(7350..8402)
                     /NRPS_PKS="Domain: Abhydrolase_1 (81-318). E-value:
                     2.2e-09. Score: 29.2. Matches aSDomain:
                     nrpspksdomains_KYK13_RS19215_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396626.1"
                     /locus_tag="KYK13_RS19215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_223646260.1"
                     /sec_met_domain="PF00561 (E-value: 5.1e-08, bitscore: 31.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPIIVPQAEGIQLFTVPLPLEEGELLGSPQIAWQAHGKPSDGKA
                     VVVLHDLSHSHQALRTEVNGAYEPSGWGRELIGPGKALDPEVTPVIVPNLLGSPFGSTS
                     PLTPDPHTGAPWGATLPPLTVLDMARGVSALLRSMELTRVRALVGVGLGGLVALRLAAL
                     FPELTDGVVVLGAARALPEGLREKLGLTSQVLRMAPDNEDTPPLRERAPNRTLARLRLD
                     YLKLLYGRDHLASAYPDSAAAQAALEAEAASFADTFDPVAWSLLCSAYAGCDLTETFPK
                     IRARVLLLVGSTDALAPAGRVRDTYHQLTAAGVQARFVELQGPGDHGTLLSDAHRLKGP
                     VQDFLRWLRG"
     aSDomain        complement(7449..8159)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KYK13_RS19215_Abhydrolase_1.1"
                     /evalue="2.20E-09"
                     /label="KYK13_RS19215_Abhydrolase_1.1"
                     /locus_tag="KYK13_RS19215"
                     /protein_end="318"
                     /protein_start="81"
                     /score="29.2"
                     /tool="antismash"
                     /translation="ALDPEVTPVIVPNLLGSPFGSTSPLTPDPHTGAPWGATLPPLTVL
                     DMARGVSALLRSMELTRVRALVGVGLGGLVALRLAALFPELTDGVVVLGAARALPEGLR
                     EKLGLTSQVLRMAPDNEDTPPLRERAPNRTLARLRLDYLKLLYGRDHLASAYPDSAAAQ
                     AALEAEAASFADTFDPVAWSLLCSAYAGCDLTETFPKIRARVLLLVGSTDALAPAGRVR
                     DTYHQLTAAGVQARF"
     misc_feature    8366..8380
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.42"
                     /tool="antismash"
     gene            8438..9367
                     /locus_tag="KYK13_RS19220"
     CDS             8438..9367
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396627.1"
                     /locus_tag="KYK13_RS19220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_223646262.1"
                     /transl_table=11
                     /translation="MKRYFGIIVVIALVIVAAVASHRTASARALEAERDADFRRIQSVY
                     LERVGWMRSNPDEASYRDELRSFFKAYFDDIDGHLDRFHGNKKFDNYLAELDKRAESGG
                     DKKDNRATDRKAFYEYARKQFDALHEGRYRPVWTATDKGMRLDVVSNDVVMVMGKPQIR
                     LQLVLWGAQRVEKDEGKVKKMVTSATFDTVWKLTDAKGKLLGEMRGADPSMKIDYPERL
                     IAEFPPQMVLGHYDLDLMPADVSKLEMTINVSSHAASGGNANATYVWKMDVPSEWKLGA
                     NETWEGATQEERPEEEIDPAKASAKKGG"
     gene            complement(9409..11604)
                     /locus_tag="KYK13_RS19225"
     CDS             complement(9409..11604)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396628.1"
                     /locus_tag="KYK13_RS19225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="WP_223646264.1"
                     /transl_table=11
                     /translation="MTRLRIGHHWKREPSASPLDSIALELDGVNLLPGAVEEPLAEVVP
                     ALVEAVAALHAGKRRLAQVSLTEAHLELVLRRVGPDIELSVASLARPAHLLRPPIRVDA
                     EELTKATRQSGQRFLQDVTKLAPKALSASAARKLGEALQGLGKAAPHAEGPPVPPTPLR
                     IEPVSVPGFGFELKDISPASREAPSKGTSGALAPLLGAGEVWLALPGRPQAWRAQGPPF
                     LTVLELSRQAVELARAVELGEARFEFEPAGVRPLLTLDLPGRRAKLGPTGTWFELEPAA
                     LLSALFQLGEALGLAFAEAHRVQVSNPYLVELAERCREGLSHLRGAHKPPEAEGEARER
                     KPSPARGESRPLKVPGKLRRLRFEKLWEQRGLEEPEEARLLLGRHGPVYCAPGLAGAFS
                     RKDGGLLWRRAASLGVAASADGHAVAADDTRVYGFTGRGAGARWLHDHDGIPLGPLLLR
                     QDGLLVTLSEDRTAVAFAEATGREVWRLAPPRTQRGWLATQGHRALLSTDSGYLYGLDL
                     EDGQVRYRLRSPLPFHGPPVPWGRRFLALMGRGSHHALLLADAHTGEAQWTFEPDLLLP
                     STPLPVGQRVLMAGMRDQDGFLVCLDARGRKVWERALHLGPGPYALAPLPRAVVVTSAS
                     GAAARVALSGQVDWRVGAAGEPLITALPARTARDVTLIAGEVVRAVDPRGGQVLAEVRA
                     GVGLVALQADVRLNLYFLDDAGTLSAYRLGSHFAVVK"
ORIGIN
        1 gccggtcgtg gtcatccccg gcttgagccc tcgcgtggcg agcagtgcat ccgtgcgctc
       61 ccgcagccct ggtgccagcc actccggcgt caacgtgaag gcccacccgt ggggcccccg
      121 cgcgaagtag ctcgcggccg agtccaccgc acgcatcagc tccgcctccg tctccgccgg
      181 ccggttgagg aacgccatgt tcatcagcga ccacgtcacg tggcccgcgg tgatgaacac
      241 gtccgcccgc tccacgacct ctccatgggg caggcccagc gtcatcagcc tccaggcgcc
      301 acggaactgc gcatgcgact cgtcgatctc ggctcgggac acgggcgtgc ctccagatgg
      361 ggtcaagaag taggattctc cgcaatcgcg gacgctcgcg tctatgtgcg agcagggagg
      421 gggcctgcta aagcgagggc tcgatccggg cgtgaggagc ggtcgcgatg aatcccatgg
      481 aagggcagcc ctggcagacg acgtgccggg tatgtggcag gcagacctgc ccgaaaccgg
      541 aggcggtggc ctcatggatg cggacgatgc tggaccgcgt gcacggcgcg cagatgcgcg
      601 gcgcgggccg ccgcggcctg cgcgaagcgc tcgcgatgga gggggtgctc atcaccacgc
      661 acctggaggt gtggtcgcgg gcgctcgagg gcgtgtgccc ggactgcatc gcggaggtgg
      721 ctccggagac ggacgcgaag ctccaggatc cagaagccat gcacgaagcg gcgctggagc
      781 gctggcgcac ctccgcgccg gagctggtca gcgacgcggt ggagtccgcg gtggatgacg
      841 tgctccaggc ggcgcgcgaa gccgccacgc gcaacaagcc gccgccctcc gagtgacgtt
      901 caggccccga gttcctgatc cagagcatcg agcaacgccg acgccacctc gggcgtgggc
      961 atccccgcct ccgtgacgag cgtcgcgtag ggctgcaccg cgacctccgc gcgcagcagg
     1021 gccccgcgca gcgcatcctg ctccgtggcg gacagccgcg cgcgcccact ggtcgatgtc
     1081 ccacgacagg cccgcgtggc gcggcttgtc ctcggcgatg cggacctggg acgccggtgt
     1141 tcacgcgcga ctcctgaggt tcgcgcgcaa ttttcaccaa gtcgttcgtc cgccccgggc
     1201 cctatgagtc ttcgcgtttc gcgacccgtc cgcgatgaca ggagacttcc cgtgcagcga
     1261 gttccatcca gaatcctccg gttcggtgcg ccgatggccg tgggaatggc gttgtccctg
     1321 ggggcgggca gcgcctccgc ttcggaccac ctcgatacgc ctacggtcat cgaggatccc
     1381 tcggcggaca tcgccgacct cttcgcctgg atgtcgcccg atggtcagcg gctgaacctg
     1441 gccatgaccg tggtcgcgca ccggttctcg gaccagctcc agtacgtgtt ccatgtcagc
     1501 agcgggaagc gcttcggaga gaccactgct gacctgtcca tccagtgccg cttcgatgca
     1561 gcgaacaccg cggagtgctg ggccggtgac gtcgaccatg tccgtggaga tgcgagccgc
     1621 gtggcgggaa tcgaagggcg tcagaagcgc ttccgcgtgt tcgcgggact gcgcgacgac
     1681 cccttcttca acaatgtgaa gggcacccgc gccgccctgg acgtggcgaa ggccgcgctc
     1741 cagggcggcg cgcccgtgga tgccgcccga tgcccccgct tcgacgcggc gaccacgcgg
     1801 tccatcctcg agcagtggcg ccagacccag ggcgggccgg gaaagaactt cctggcgggc
     1861 tggtcgtctt cggcgctggt ggtctccatc gaccgtgacg ccgtcggcaa ggcagggccg
     1921 tacctcgcgg tgtgggcagg gatctacaag agcaacccgg acgcacggaa ggggacctcg
     1981 gctcggccga agctcggtgc gccggtggat cgcatcgggc ggccgctgac gggcaatgcg
     2041 ttgatcggtc cgctggagcc ggatgacgtg agcgaccggc gcaaggagga atacaaccgc
     2101 gcggctcccg cggactgggc tggattcgca ccggacctgc agcgcacgct cgggctctac
     2161 gacgggctgg atggaacgtg tgggaaccag tggctcgcca atgcgggtgg cgaacccgcg
     2221 cagcgatatc agcgcctcgc gaagctgctc gcggacgacc ggctgtggat caacagccgg
     2281 gccactgtgt gcaagcagta cctctctgtc gaactggcag agctttcgac tccggggaag
     2341 ccgggcaccg actgcggcgg caggacgccc ctcgaagact cgaacgacgt gttccggtcg
     2401 ctcctcgctc tcggcgcgac gtccggtatc gatgacggag tgaaggagga cgaccatgtc
     2461 cattccctgt ccgacttccc gttcctggcg gccccatgag accgagtgaa tcctggagca
     2521 cgtggctggc agtcgcggtc atggtctgcg gcggagccac cgccgcgtgg ggagaggctc
     2581 cgcggtacgt cacgaccacg ggggccctgg cgatcgagaa cctcgaccac cgcatcgcgc
     2641 tccaacgcgc ggacgcgggc gtcggggagc tgctgctcct gcgggcgcga ttcctgagcg
     2701 actacgacgc actggaccgc gcgagcacgc tcgcggaaga acgttcgagg acggcgggtg
     2761 acctcctgga gcgtgcgcgc gcacgctcgg cggtccaccg gttcgcggag gcgctcacgg
     2821 acctggacgc agccggaaag gatgacgaag cgagcagcga tctgcgtgct tcgatcctcg
     2881 tcgcgaccgg acacgcgagc gaagcgatct cccggctcga ggcaagggtg gcgcagcgtc
     2941 ccggctacgc gtcgcggagc gcactcgcgg gcgcctacgc ggcggtgggc cgcttcaccg
     3001 acgcggaccg gctgtattcc gaagcgctcg cggacctcga caccacttca ccgtttccct
     3061 acgcctggat ctacttcgcg cgcggcgtga tgtgggccga acaggctgga gaccccgcac
     3121 gcgccgagcc gctctatgcc cgggcgctcg cccatctccc cgagttcgcg atggcgaacg
     3181 tgcacctcgc cgagctcgag gccgcgcgtg gcgacgtggc gtccgcgatg acacggctcg
     3241 aacgcgtcgt cgcttcgacg cacgagcccg aggccctggc actcctggga acgctgcacc
     3301 ttcgcaccgg tgcaccggtg cgcggccgga gcgaaatcac cgccgcgcgc gagcgcttcg
     3361 aagcgctcct ggtccgccaa ccgctggcct tcgcggacca cgccgcgagg ttctacctgg
     3421 ggccgggcgc ggacccggag cgggcctggg tgctggcgca acagagcctg gagggccgtc
     3481 aaaccgccag cgccttcacc ctggccatcg aggccgcgcg tgcgacggga cgcgagtgcg
     3541 aggcctgcgt gctcgtcgaa gcgagccggg cccggggctt gccagtcccc ggggacggat
     3601 gcccgtagcg cgctgcctca ggccgactgg ggcgcgcgga acaagggcgc acccagcgcc
     3661 gcaccgttga cgccgccatc gacgaacaga tcctgcccgt tcacgtagga cgcttcgtcg
     3721 gatgcgagaa acagcaccgt cctggcgatc tcctccggct tgccgatgcg ccccaggggg
     3781 atggtggcgg acatgcggcg ctccagcttc gcgaaggact ccggcgtggg cgcgtccttc
     3841 tcccagatgg gcgtgttcgt cgcgccaggg gacacgacgt tgacgcggat gccctgtggc
     3901 gcgagctccg ccgcgagcac gctcgccatc gccttcagcg cgcccttgct cgccgcgtat
     3961 gcggagtagc ccggcatccc caactcgctg tgcacggagc tggtgaggat gaccgacgcg
     4021 ccgggcttca ggtgcgcggc cgcggcctgc accgtgaaga acaccccggt gacgttggtc
     4081 tggagcacgg acgtgaacgc ctcatgcgac gtcctgccca cgggcgtccg ttccgcgatg
     4141 ccggcgttgg cgaacacgat gtccaatccg ccgaagcgct ccaccgtcgc cgccaccgcg
     4201 cgctccaggg cggatggctc cgtggcgtcc gcctgcaccg ccagcacgtt cgcgcccagc
     4261 ttcttcacca ccgcgtccag cgtctcccgg ttgcggccgg tgatggccac ccgcgccccc
     4321 tccgccacga acagccgcgc cgtcgccagc ccgatgccac tgttaccgcc cgtgatcagc
     4381 gccgtctttc ccttgagccg catccgcctg cctccgcgtt tttggtttca tgatgaaacc
     4441 aatcgccgga tgaataaaaa ccctggtttc gttatgcaac cagttgtgga tgagcccgag
     4501 gaaggaggcg gcggtgaagc ggacgagcat ggaaggggtg atgtgcccgg tggcccggtc
     4561 gctggacgtc atcggggact ggtggtcgct gctcatcgtg cgggatgcgc agtacggcct
     4621 gcgccgcttc ggtgagttcc agaagagcct gggcgtggcg aagaacatcc tggcccagcg
     4681 cctgcggcac ctggtggacc acggcatcct cgaaacgcag ccggcctccg acggcagcgc
     4741 ctggcaggag tacgtgctga cggagaaggg ccagagcctc tttcccatcc tggtcgcgct
     4801 ggggcagtgg gggcaggagc actgtttcca ggcgggcgaa ccccgcacgc gggtgctcga
     4861 caaggcgcac ggaaagccgg tgaagccgct ggaggtgcgc gcggcggacg gccgggtgct
     4921 gacgctgaag gacgtgcgcc tggagcggcc gccctcacaa gggtagcgcc acccggagcc
     4981 gggctggtta gggtgtgccc atgacgcgcc ctgcatcgga ttcgtggtca ctgccctgga
     5041 tggggctggg gctgagcagc aacctgagcg cctcggacgt gccgcagccg taccggttgc
     5101 tcgatgcctc gcccggcctc ttcgacttcg tggagtacag cgcgcccatc gcgctggagg
     5161 aggcgaaggc ccaggccacg ctctttccgg agatgtggcg ccggcgctcg gaggtgccgg
     5221 tcctcttcca tcccgtgcac ctgaacctgt gggggccgga gctggagccc gcgtccgcgc
     5281 tcgcgcagct ggacgcgcac gcgcgcgcgg tgggcagccc gtgggtgggc aacgacgtgg
     5341 gctggtggca cgcgggcggt cagcccttcc cgggctacct ctatgtcacg ccgccgttca
     5401 acgaagcggg cgtgcgggac agcgcggcgc acgcgctcca catccaggcg ggcttgagcg
     5461 tcccgctggt gattgagaac cccgcggtgc tcgccacgcg cggtgggatg cacgcgctgg
     5521 acttcatggc gaagctgcac gcgcgcacgg gcctgccgct gctcctggac ctgggccacc
     5581 tcttcagcca ccagctctcc gcgggcctgc ccctgcgcac ggggctggat ggcttcccgc
     5641 tggatcaggt cgtggagctc cacatcgcgg gcggcgtggt gacacgcagg ggagggcgga
     5701 ccttctacgt ggatgatcac acgcagcccg tgcgcgagga gctgttcgaa ctgctggcgg
     5761 aggtcctccc gcgctgcccg cgcctgcgcg ccgtcacctt cgagggcgat ggccatccgc
     5821 cggaggtcgc gctcgcgtcg ctgcggaggc tgcgcgcgct tgtgacagga gcgtcacggc
     5881 ccgccatcga cgtccccgcg tcgcgcgcgc ccgtgcccgc gctcaccggg gacagccgcc
     5941 catgggcgct cttcgacgca gggcacggcg tcacgccggc cgcggaggac gaggaccccg
     6001 agggcacgcg cgcggaccgg gacttccgcc tggcggtggt ggcggagcag ctggacaagg
     6061 agtggcccct cacgcgcctg ctgctcgcgg cgacgccgga ggggctggag tccttcaccc
     6121 gctcgcgcga gtaccgaggg ctcttcgacg ggctgggcag gtcgctgtcc cacgccttcg
     6181 cgtcctgggc ccgcaagcgc gtgatggcct cgccgtcgga cggggtggcc gccgcgctgt
     6241 ccctggagat gatgctgccg cacgccttcc tccaggagcc tccggccccc gcgccgggcc
     6301 aggtggcgct ggccgaggac gtccggctgg gctcgttccc cgcggacctg acggagctcg
     6361 tcttcgccgc acgggcgttg cggcggcacc tcaccggccg cgcatgggcc tgtggggcgc
     6421 tggaggtgtc cggcctggag gccctggcgc aggtggcggc ccggccggga cccggcccct
     6481 ggcgcttcgc catccgccgc aaggccccgg gcttcgaggt cctgacgctc ccgccggagg
     6541 tggccgaggg gcttcggggg ctcgcggacg gcccgctccc ggcggagtcc ctgcccggat
     6601 ggctgctcgc ggaagggcag gcgcgcggtc tcttgcggcg ggcataaggg gcggcagtgt
     6661 ccgtaggtga acggaccctt gcgcccacct gtctggtggg gtagtgcccg ccgcatgtcg
     6721 tcacccctcg tcgttggcat cgcgggcggc accgcgtccg gcaagaccac ggtggcccgc
     6781 aaggtgcgtg aggcccttgc cgactgccgg gtcgccttca ttgatcagga ttcgtactac
     6841 cgggacctga aggacatgtc cctcgaagag cggcgggagg tgaacttcga ccatcccgac
     6901 gccttcgaca cggagctgtt ggtcagccac ctgcgcgcgc tcaagcaggg ccacgccatc
     6961 cagaagcccg tctacgactt cgtcacgtcc ggccgtcagc cgcacacgca ccgcgtggac
     7021 cccggggaca tcatcctcat cgagggcatc ctcgtgctcc acatgaagga ggtgcgcgac
     7081 gagatggacg tgaagatcta cgtcgacgcg gacgacgacc tgcgcatcct tcgccgcctc
     7141 acccgcgaca tcaaggaccg cggccgcgac ttcgaccacg tggtgggcca gtacctgcgc
     7201 cacgtgcgcc ccatgcacat gggcttcgtg gagccgtcca agcaccacgc ggacatcatc
     7261 atcccgcacg gcggcaacaa cgacatcgcc atcgggatgc tggtgggcgc gctccgggcg
     7321 cggctcgcca cgcagcatca ggcccagcgc tagccacgca gccagcgcag gaagtcctgc
     7381 accggcccct tgagccggtg cgcgtccgac agcagggtgc cgtgatcccc cggcccctgc
     7441 agctccacga accgcgcctg cacccccgcc gccgtcagct ggtggtaggt gtcccgcacg
     7501 cggcccgccg gggccaacgc gtccgtcgag cccacgagca ggagcacccg cgcgcggatc
     7561 ttcgggaacg tctccgtcag gtcgcagccc gcgtacgcgg agcacagcag cgaccacgcc
     7621 accggatcga acgtgtccgc gaatgacgcg gcctccgcct ccagcgccgc ctgcgccgcc
     7681 gcgctgtccg gatacgcgct ggccaggtgg tcgcgcccgt agagcagctt gaggtagtcc
     7741 agccgcagcc gcgccagcgt ccggttcggc gcgcgctcgc gcaggggcgg ggtgtcctcg
     7801 ttgtcgggcg ccatgcgcag cacctgcgac gtgagcccca gcttctcgcg cagcccctcc
     7861 ggcagcgccc gcgccgcgcc cagcaccacc acgccgtcgg tcagctccgg gaacagcgcc
     7921 gcgagccgca gcgccaccag ccctcccagg cccacgccca ccagggcccg cacgcgcgtg
     7981 agctccatgg agcgcaggag cgcggacacg cctcgcgcca tgtccagcac ggtgagcggc
     8041 gggagcgtcg cgccccacgg cgcgccggtg tgcggatccg gcgtcagcgg cgacgtggag
     8101 ccgaacgggc tgcccagcag gttgggcacg atgaccggcg tcacctccgg atccagcgcc
     8161 ttgcccgggc cgatcagctc gcgcccccag cccgaaggtt cgtacgcacc gttcacttcc
     8221 gtgcgcagcg cttgatggga gtgcgacagg tcgtgcagca ccaccaccgc tttgccgtct
     8281 gacggtttcc catgggcctg ccaggcgatc tggggacttc ccagcagctc cccttcctcc
     8341 agcggaaggg gcacggtgaa cagttggatt ccctcggcct ggggcacgat gatggggctc
     8401 atatcattgc ctgcccgtac gcacaggagc ctgtcgcttg aagcgctact tcggaatcat
     8461 cgttgtcatc gccctggtga tcgtcgccgc agtggcgagc caccgcaccg cctccgcccg
     8521 ggcactcgag gcggagcggg acgcggactt ccgccggatc cagtcggtct acctggagcg
     8581 ggtggggtgg atgcgctcca acccggatga ggcgtcctac cgggacgagc tgaggtcgtt
     8641 cttcaaggcg tacttcgacg acatcgacgg ccacctggac cgcttccacg gcaacaagaa
     8701 gttcgacaac tacctggcgg agctggacaa gcgcgcggag tcgggcggcg acaagaagga
     8761 caaccgcgcc acggaccgca aggccttcta cgagtacgcg cgcaagcagt tcgacgccct
     8821 gcacgagggc cgctaccgcc cggtgtggac cgccacggac aagggcatgc gcctggacgt
     8881 ggtgtccaac gacgtggtga tggtgatggg caagccgcag atccgcctgc agctggtgct
     8941 ctggggcgcg cagcgcgtgg agaaggacga gggcaaggtg aagaagatgg tcaccagcgc
     9001 cacgttcgac acggtgtgga agctcacgga cgcgaagggc aagctcctgg gcgagatgcg
     9061 cggcgccgac ccgtcgatga agatcgacta ccccgagcgc ctcatcgccg agttcccccc
     9121 gcagatggtg ctgggccact acgacctgga cctcatgccc gcggacgtgt cgaagctgga
     9181 gatgaccatc aacgtctcct cgcacgcggc gtcgggcggc aacgccaacg ccacctacgt
     9241 gtggaagatg gacgtgccgt cggagtggaa gctgggcgcc aacgagacgt gggaaggcgc
     9301 cacgcaggag gagcggccgg aggaggagat cgacccggcc aaggcctccg ccaagaaggg
     9361 cgggtagccc ttcgtttcgg atgaagggcg gggagccctt cgtcgtcgtc acttcaccac
     9421 ggcgaagtgc gaacccagcc ggtacgcgga cagcgtgccg gcgtcgtcca ggaagtacag
     9481 gttgaggcgc acgtcggcct ggagcgcgac gagccccacc cccgcgcgca cctccgcgag
     9541 cacctgaccg ccgcgaggat ccaccgcgcg caccacctcg cccgcgatga gcgtcacgtc
     9601 gcgggcggtg cgcgccggca gcgcggtgat cagcggctca cccgccgcgc ccacgcgcca
     9661 gtccacctgt ccggacaggg ccacgcgagc ggcggccccg gatgcgctcg tcaccaccac
     9721 cgcgcgaggc agcggcgcca gcgcgtaggg cccggggccc aggtgcagcg cgcgctccca
     9781 gaccttgcgt ccgcgcgcgt ccaggcacac caggaacccg tcctggtcgc gcatcccggc
     9841 catgagcacc cgctggccca ccggcagcgg cgtggagggc aggaggaggt ccggctcgaa
     9901 cgtccactgc gcctctccgg tgtgcgcgtc cgcgagcagc agcgcgtggt gcgagccgcg
     9961 ccccatgagc gccaggaagc gccggcccca cgggaccggg ggcccatgga agggcagcgg
    10021 tgagcgcagc cggtagcgca cctggccgtc ctccaggtcc aggccgtaca ggtagcccga
    10081 gtccgtggac aggagcgccc ggtgtccctg cgtggccagc cacccgcgct gcgtgcgcgg
    10141 cggggccagg cgccacacct cgcggcccgt ggcctccgcg aaggccaccg ccgtgcggtc
    10201 ctccgacagc gtcacgagca gcccgtcctg gcgcaggagc agcggtccca gcgggattcc
    10261 gtcatggtcg tgcagccatc gggcccccgc gccccggccg gtgaagccgt acacgcgcgt
    10321 gtcgtccgcc gccaccgcgt ggccatccgc ggacgccgcc acgcccagcg acgcggcccg
    10381 gcgccacaag agcccgccgt ccttgcgcga gaacgccccc gccagccccg gcgcgcagta
    10441 caccggccca tgccggccca acagcagccg cgcctcctcc ggctcctcca gcccgcgctg
    10501 ctcccacagc ttctcgaagc gcaggcgcct cagcttcccg ggcaccttca gggggcggga
    10561 ctcaccgcgc gccggggaag gcttgcgctc gcgtgcctcg ccctcggcct cgggcggctt
    10621 gtgggcgccc cgcaggtgcg acagcccctc gcggcagcgc tccgccagct ccacgaggta
    10681 cgggttggag acctggaccc ggtgcgcctc cgcgaacgcc aggcccagcg cctcgcccaa
    10741 ctggaagagg gcggacagga gcgccgccgg ctccagctcg aaccaggtgc cggtgggccc
    10801 cagcttcgcc cgccgtcctg gcaggtccag cgtcagcagg gggcgcacgc ccgcgggctc
    10861 gaactcgaag cgggcctcgc ccagctccac cgcccgcgcc agctccaccg cctgccgaga
    10921 cagctccagc accgtgagga acggcggccc ctgcgcgcgc cacgcctgcg gccgtccggg
    10981 cagcgccagc cacacctcgc ccgcacccaa cagcggcgcc agcgccccgg aggtgccctt
    11041 cgagggcgcc tcgcgcgagg cgggggagat gtccttcagc tcgaagccga accccggcac
    11101 ggagacgggc tcgatccgca gcggggtggg gggcaccggt ggcccttccg catgcggggc
    11161 ggccttgccc aggccctgga gcgcctcgcc cagcttccgg gcggccgagg cggacagcgc
    11221 cttgggggcg agcttcgtca cgtcctggag gaagcgctga ccgctctggc gggtggcctt
    11281 cgtcagctcc tcggcgtcca cccggatggg cgggcgcagc aggtgcgcgg gccgggcgag
    11341 gctggccacc gacagctcga tgtccggccc gacgcgccgc aacacgagct ccaggtgcgc
    11401 ctccgtcagt gacacctgcg ccagccgccg cttccccgcg tgcagggcgg ctaccgcctc
    11461 caccagcgcg gggacgacct ccgccagggg ctcctccacc gccccgggca acaggttcac
    11521 cccgtccagt tccagcgcga tggaatcgag cggggaggct gacggttcgc gcttccaatg
    11581 gtgtccgatg cgaagacggg tcattgacgt tcgttgacaa gccagtttag cggtggctag
    11641 catccgc
//
