LOCUS       NZ_CP079946            11476 bp    DNA     circular CON 18-AUG-2024
DEFINITION  Corallococcus sp. EGB chromosome, complete genome.
ACCESSION   NZ_CP079946
VERSION     NZ_CP079946.1
KEYWORDS    RefSeq.
SOURCE      Corallococcus sp. EGB
  ORGANISM  Corallococcus sp. EGB
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Corallococcus.
REFERENCE   1  (bases 1 to 9431171)
  AUTHORS   Zhao,Y. and Cui,Z.
  TITLE     Whole-genome sequencing of Corallococcus sp. strain EGB reveals the
            genetic determinants linking taxonomy and predatory behavior
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9431171)
  AUTHORS   Zhao,Y. and Cui,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2021) College of Life Science, Nanjing
            Agricultural University, No.1 Weigang Road, Nanjing, Jiangsu 210095,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. MAY-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 113.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019968905.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 04:02:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,752
            CDSs (total)                      :: 7,680
            Genes (coding)                    :: 7,614
            CDSs (with protein)               :: 7,614
            Genes (RNA)                       :: 72
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 12 of 66
            Pseudo Genes (incomplete)         :: 62 of 66
            Pseudo Genes (internal stop)      :: 2 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:46:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 216206
            Orig. end                         :: 227682
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079946.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from College of Life Science,
            Nanjing Agricultural University, China.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11476
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11476
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11476
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6476](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6476)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(566..1471)
                     /locus_tag="KYK13_RS00935"
     CDS             complement(566..1471)
                     /NRPS_PKS="Domain: Abhydrolase_1 (48-288). E-value: 7e-35.
                     Score: 112.7. Matches aSDomain:
                     nrpspksdomains_KYK13_RS00935_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262: haloalkane dehalogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393062.1"
                     /locus_tag="KYK13_RS00935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_223641006.1"
                     /sec_met_domain="PF00561 (E-value: 1.2e-26, bitscore: 92.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 6.1e-20, bitscore:
                     71.6, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRQQPNPTTTPAKAPSAVRPFFPQGFREGDEDVNGTRIHFVIGG
                     EGRPVLLLHGYTQTHLMWWRLAPGLAKQHTVILPDLRGAGASAAPEQGYDKETLARDMR
                     ALVKKLGFDKVSVVGHDIGLMVAYAYAALFPNEVERLALLDAFLPGIEPWSDQVMRSRD
                     VWHFTFNGPTAEKLVRGRERVYFDHFWTDFAANPHAVDEAERQAYTEAYAAPGRLHSTW
                     SYFQAFDQDKKAFRELARNKLPMPVMVIGGDKSLGGPLAAQARAVATQVEPHILPDTGH
                     WVAEERPEEVRKLLGGFLRA"
     aSDomain        complement(608..1327)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KYK13_RS00935_Abhydrolase_1.1"
                     /evalue="7.00E-35"
                     /label="KYK13_RS00935_Abhydrolase_1.1"
                     /locus_tag="KYK13_RS00935"
                     /protein_end="288"
                     /protein_start="48"
                     /score="112.7"
                     /tool="antismash"
                     /translation="PVLLLHGYTQTHLMWWRLAPGLAKQHTVILPDLRGAGASAAPEQG
                     YDKETLARDMRALVKKLGFDKVSVVGHDIGLMVAYAYAALFPNEVERLALLDAFLPGIE
                     PWSDQVMRSRDVWHFTFNGPTAEKLVRGRERVYFDHFWTDFAANPHAVDEAERQAYTEA
                     YAAPGRLHSTWSYFQAFDQDKKAFRELARNKLPMPVMVIGGDKSLGGPLAAQARAVATQ
                     VEPHILPDTGHWVAEERP"
     gene            complement(1577..2014)
                     /locus_tag="KYK13_RS00940"
     CDS             complement(1577..2014)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393063.1"
                     /locus_tag="KYK13_RS00940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="WP_223641008.1"
                     /transl_table=11
                     /translation="MSSISDETHERITDLCAQGDAAAGEDDFEQALKHFKAALALIPAP
                     THAHQQNTWVRAAIGDMYFQLERFEDCREHFREAVRSPGGLGNPFIHLRLGQCALELGD
                     EARAADELARAYMGGGEELFDGDDAKYLEFIQSRLKPPHDA"
     gene            complement(2060..2866)
                     /locus_tag="KYK13_RS00945"
     CDS             complement(2060..2866)
                     /EC_number="3.5.1.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393065.1"
                     /locus_tag="KYK13_RS00945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PIG-L deacetylase family protein"
                     /protein_id="WP_223641010.1"
                     /sec_met_domain="PIG-L (E-value: 2.5e-18, bitscore: 65.9,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTALFVSPHLDDVAFSCGGTLAALKAKRWTVALVTVFTRSVPEP
                     KGFALQCQTSKGLGPEVDYMALRRDEDRAFAACMGVDHVRWGDLEEAPHRGYASPEALF
                     QPPRADDTIEARVAACLKPVLWDLKPDRVFVPQALGHHVDHVQVVRAVKGLGIPSNRIL
                     YYRDTPYAVRQPRAHPDAAVPQGLRPLTVDITEYLPKKVEGCIRYGTQLGFQFGGVEGL
                     ARTLTAFHRMEAQARGQPGAAEVFLAEPSQGAPPPPVTKTSPPATR"
     gene            complement(2863..4149)
                     /locus_tag="KYK13_RS00950"
     CDS             complement(2863..4149)
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393066.1"
                     /locus_tag="KYK13_RS00950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_223641014.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 3.6e-32,
                     bitscore: 110.2, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSVNRLRICFISRRFFPAISGMSVYALNLVRELVASGHDVTMVS
                     QYRNDPAGMAVYGGGPPPGIPGAQVLGCESLGEQRINQGQPASFEQDLEVMVDTVVREH
                     RRRPFDLIHAQYGYPCGLAALEAARRLGLPNVVSIQGGDGHWVGTCCGTHKQAMLAVLG
                     HAGALLIGSRSFAEEVRGHHGTPLERFTIVPGATDTQRFHPRNESALGQPRDPPVWLYH
                     GRVDARKGVMELLDAARRLVADGRRFKLRVSGIGPDVAAVRARVEEGGLQDVVELTGVS
                     SYAEAPDLYRSGDLFVSPTYSEGFSNTLLEAMASGLPIVSTRAVGVVDCLEDGRDALLV
                     PPKDSVALAEAMGRLMDDAPLRQRLAATALREVRELYSWQSVGRRIQGVYAELTGTRPD
                     TGWTHLYDPAMTEERADHTCRFRAAPHLL"
     misc_feature    complement(2866..2868)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4146..4997)
                     /locus_tag="KYK13_RS00955"
     CDS             complement(4146..4997)
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615746.1"
                     /locus_tag="KYK13_RS00955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="WP_223641017.1"
                     /transl_table=11
                     /translation="MALRFAYNTNGVSHHRFEDALSLIADSGYDGVALTLDHHHFDPFA
                     PDFGQRTEQLATRLERLGLGLVVETGARFLLDPRRKHEPTLITPDAEGRARRLEFLKRA
                     VDVCGTCRGEAVSFWAGVPLPGVTEALAWPWLEEGVARLAEYAAARGVVLAVEPEPGML
                     VETVDGWRRLHARVPGVRLALDVGHLLVTQERTPAEAVREFAPVLGTVALEDMKRGVHE
                     HLPFGEGDVDVPSVLRELTRAGYGRLVCVELSRDAHRAHDMVPQALEWLRARLPTGAEV
                     AA"
     gene            complement(5001..6476)
                     /locus_tag="KYK13_RS00960"
     CDS             complement(5001..6476)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393068.1"
                     /locus_tag="KYK13_RS00960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_223641020.1"
                     /sec_met_domain="YcaO (E-value: 1.4e-61, bitscore: 207.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVARALDAYHRAMSTGALQLFRIDPIDRLGIPVASASLRLGNDA
                     GAVLHGNGYGRTEEEAQVGALGELVEETFSELAMRRMPRVHGSYAALVRARGPTAVADP
                     LTLGLPAGSPYQPEMPLIWVEMQRLSTGERVLVPEEYVAIHPGQLQGRAPLITPITNGQ
                     GAGLTRPQALAHGLLELLQRDGNGLVYRAMDQGVALDLEGADLPPDVRELLERYRRAGV
                     EVMAKLACTDFGLVNLYVVGRDVSVGNQPLMVTACGEAADPDRDRALRKALLEYACSRA
                     RKAFMHGPLSEVARVAPQEYMDRFVPMVDLEAEEPRALNAMVEWARLSAPELSELTACT
                     LMVRQKVRFEDLPRAPALEDPSLRCDHVVRQLHASGLDILVADLSPPGRQVHVVKALVP
                     GLEVETMTYNRVGERNVARLLERRDPLVGLGAPPPGAEPVRLTPEAELRLGGPAWFNVR
                     LAEARVGRLYALYREPDRHAVPAVLSSRRFGGQ"
     gene            complement(6473..7306)
                     /locus_tag="KYK13_RS00965"
     CDS             complement(6473..7306)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615760.1"
                     /locus_tag="KYK13_RS00965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_223641022.1"
                     /transl_table=11
                     /translation="MPLTRYACTHCGTWQPGFPREKPMGCPTCLDVRNALPREGWEFQS
                     AGQVSERLGTRWHEALPGIWGFTCTPPFGLGSTGWLLRRPEGNVGFEGAPWYSRPALEF
                     IEVMGGLHVLSASHPHGFGALWQLQEHFDPLLVLHREAVPYSKAFQVRWPVDDEHALAP
                     DLTLHCVGGHYEGHMVLYDARTRSLFCGDALKVDLDARGRPTALSCHKGFHYAIPLSHG
                     ELRRYRAVLEALPFENVFTPFEFARGVTREHALALFDRLLAGMPHTRPLPLEELS"
     gene            complement(7297..8331)
                     /locus_tag="KYK13_RS00970"
     CDS             complement(7297..8331)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA_C3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393070.1"
                     /locus_tag="KYK13_RS00970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="WP_223641024.1"
                     /sec_met_domain="GFO_IDH_MocA (E-value: 2.2e-26, bitscore:
                     91.7, seeds: 39, tool: rule-based-clusters)"
                     /sec_met_domain="GFO_IDH_MocA_C3 (E-value: 5.3e-30,
                     bitscore: 102.8, seeds: 4226, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFEAKPGTRSLGWAVVGCGWVARDYVIPALQGASNARLVALCDVS
                     AEALMRIRADGVNRYTALGSVLADKDVQAVYIATPNHLHVAMTEACAAAGKHVLCEKPM
                     AITARDGLRMVSACQRAGVHYATAFDQRHHAAHRRLRTLVKEGALGTITQARIHYACWL
                     PEDWAPDNWRIDPEQAGGGAMIDLAPHGLDLLEVVLGDEWASLSALTQRRVHTYAVDDG
                     AVLMGQFKSGTLGLLQVAYNCPDTYPRRTLELIGTKARALAYKTMGQTPGGTLTLTDAK
                     TGGETRIILPPEEDPSPFLHQVESFSACVLEGRPQPYAPERDVRLVGLLSQATRDGRAF
                     PPCH"
     gene            complement(8374..9306)
                     /locus_tag="KYK13_RS00975"
     CDS             complement(8374..9306)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393071.1"
                     /locus_tag="KYK13_RS00975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_223641026.1"
                     /transl_table=11
                     /translation="MNAPAHDTAAAGRLAELRPLLSELMDLKRIRTPDHPDGLAAHGFR
                     RAWASLASGMAPRTVALQETARAVAAVRLGGLDMDVLQRAGLSPLDAARVLHRGLEAVA
                     APLDARLKERLSVALAQTPEEACHAPPPWFVERLVRQPRAGATFPGRPRILVSPQEGHA
                     DHCYAVAVGAVLVAPRFGASAALPFVAGLSHHLFNAALPDAGYGGEALLGDLLEPAMKR
                     LTEEALAALPERLAGVVRQALTLTGNVDSAEARAFNAADALDRVLELEAHARAAGFTLR
                     QAMEDLELIHPGPLQTFGNDVLREAAVWP"
     gene            complement(9312..10538)
                     /locus_tag="KYK13_RS00980"
     CDS             complement(9312..10538)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393072.1"
                     /locus_tag="KYK13_RS00980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="inositol-3-phosphate synthase"
                     /protein_id="WP_223641028.1"
                     /transl_table=11
                     /translation="MAGDERLGVAVVGLGGAVATTAVAGMELLRRGRVDTRGLPLADAK
                     GMGLVEYGALTFGGWDLFEDDLARAARNHAVLTEAQLEAVAPTLGRMRPWPAASNARFC
                     KNVVGTATKNANTLREQVRAIREDLTRFKQAEQLDRVVVVNLASTEKAVDLTRPEFATP
                     ESFEKALDANHPDIGPAMLYAYAAIGDGIPFANFTPSVAADVPALLMLAKRTGAPVAGK
                     DGKTGQTLLKTVLAPALRDRALHVDGWYSTNILGNRDGEALNDPASRQNKLDTKGAALD
                     SILGYKVQDHVVSIQYYRPRGDNKEAWDNIDVTGFLGQPMQLKLNFLCKDSILAAPLVV
                     ELARTLDLAKRRGECGVIDALGGFFKAPMSQDGQPVEHAMPEQQRRLMAWLSRGRAQQV
                     ERLPERIRG"
     misc_feature    complement(10656..10670)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.93"
                     /tool="antismash"
     misc_feature    complement(10766..10782)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.03"
                     /tool="antismash"
ORIGIN
        1 cgggcacaac ctggtgctgg acgcgctcct ggcgagccag cagaaggacg gcgtgctgtt
       61 ccacacgcgc tacctgaatg gccacgtgct caatcccttc cggccgttgg aggacgtggt
      121 gcggatgcac tccaagaact actcggcgga caacgggacg ccgctgtacg accaggcggt
      181 ggtgctgctg ggcacggtgc tggcgaaggc gcaggagttc agcggcaacg gggtgccggt
      241 gcgcacggtg acgctgctga tcaccgacgg cgcggaccag ggctcgcaga aggcgcgggc
      301 gaaggacgtg gcggcgctgg tgaaggacct gcaccgggtg gagaaccaca tcgtcgcggc
      361 gatgggcatc gacgatgggg tgacggactt ccggcgcgtg ttccaggaga tgggcatcgc
      421 ggaccggtgg atcctgacgc cgggccagag cgcccaggag atccgcgccg cgttccaggt
      481 gttcagccag tccgccgtgc gcgtcagcca gggcgcggcc agcttcagcc gcgcggcgct
      541 ggggggcttc ggggcctgac gggcgtcacg cccggaggaa gcctccgagc agcttgcgga
      601 cctcctcggg gcgttcctcg gccacccagt ggccggtgtc cgggaggatg tggggttcga
      661 cctgggtggc caccgcacgc gcctgtgcgg cgagcggtcc gcccagggac ttgtcacccc
      721 caatgaccat cacgggcatg gggagtttgt tccgggccag ctcccggaag gccttcttgt
      781 cctggtcgaa cgcctggaag tagctccacg tcgagtggag ccggcccggg gccgcgtagg
      841 cctccgtgta ggcctgccgc tccgcttcgt cgaccgcgtg gggattggcg gcgaagtcgg
      901 tccagaaatg gtcgaagtag acgcgctcgc gtccccgcac cagcttctcc gcggtggggc
      961 cgttgaaggt gaagtgccac acgtcccggc tgcgcatgac ctggtcggac cagggctcga
     1021 tgcccggcag gaacgcgtcc agcagggcca gtcgctcgac ctcgttgggg aacagggcgg
     1081 cgtaggcgta ggcgaccatc aggccgatgt cgtggcccac gacggagacc ttgtcgaagc
     1141 cgagcttctt caccagcgcg cgcatgtccc gggccagggt ctccttgtcg tagccctgct
     1201 ctggcgcggc cgaggcgccc gcgccgcgca ggtccgggag gatcacggtg tgctgcttcg
     1261 cgagccccgg cgccaggcgc caccacatga ggtgcgtctg cgtgtagccg tgcagcagca
     1321 ggacggggcg tccctcgccg ccgatgacga agtggatccg cgtgccgttg acgtcctcgt
     1381 cgccttcgcg gaagccctga gggaagaagg gccggacggc gctcggagcc ttggctggag
     1441 tcgtcgtggg gttcggctgc tggcgtgtca tcggcgcctc cgcgcgagag cgggttcacg
     1501 cctcagggtg tgcacgcctc gcgtccggga ggtgccgggc cgagcgggga gtccggcgct
     1561 cgggctcctg ggtccctcat gcgtcgtggg gcggcttcag gcgggactgg atgaactcca
     1621 ggtacttcgc gtcgtcgccg tcgaagagct cctcgccgcc acccatgtag gcgcgtgcca
     1681 gctcgtcggc cgcgcgggct tcgtcaccca gttcgagggc gcactggccg agccgcaggt
     1741 ggatgaaggg attgccgagc cctcccggtg agcggacggc ttcgcggaag tgctcgcggc
     1801 agtcctcgaa gcgctcgagc tggaagtaca tgtcgccgat ggcggcgcgc acccaggtgt
     1861 tctgctggtg ggcgtgggtg ggggcgggaa tcaaggcgag ggcggccttg aagtgtttca
     1921 gggcctgctc gaagtcgtcc tcgcccgcgg cggcgtctcc ctgggcgcag aggtcggtga
     1981 tgcgttcgtg ggtttcatcc gagattgacg acatcgttgc tccggggacc ggggagacat
     2041 tcgcggtcct cggagcactc taccgagtcg caggcgggga cgtcttcgtc acgggaggag
     2101 ggggcgcccc ttgtgagggc tccgccagga acacctccgc cgctccgggt tgcccgcgtg
     2161 cctgcgcttc catccggtgg aacgccgtga gcgtccgcgc caggccctcc acgcctccga
     2221 actggaagcc cagctgcgtg ccgtaacgga tgcagccctc caccttcttc ggcaggtatt
     2281 ccgtgatgtc caccgtcaag ggacgcaggc cctggggcac ggcggcgtcc ggatgcgccc
     2341 tcggctgcct tacggcgtag ggcgtgtccc ggtaatagag gatccggttc gaggggatgc
     2401 ccagcccctt caccgcccgc accacctgca cgtggtccac gtgatgcccc agcgcctggg
     2461 gcacgaagac ccggtccggc ttcaggtccc acagcaccgg cttcagacac gccgccaccc
     2521 tggcctcgat ggtgtcgtcc gctctcggcg gctggaacag ggcctccggg ctcgcgtagc
     2581 cccggtgcgg cgcttcctcc aggtctcccc acctcacgtg gtccacgccc atgcaggccg
     2641 cgaaggcgcg gtcctcgtcc cgtcgcaggg ccatgtagtc cacctccggt cccaagccct
     2701 tcgacgtctg gcattgcagc gcgaagccct tcggctccgg cacggagcgc gtgaagacgg
     2761 tgacgagcgc caccgtccac cgcttcgcct tcagcgccgc caacgtgccg ccacaggaga
     2821 aggccacgtc gtccaggtgc ggtgacacga acagggccgt gctcataaca ggtgcggcgc
     2881 ggcccggaac cggcacgtgt ggtccgcgcg ctcctccgtc atcgccgggt cgtacaggtg
     2941 cgtccatccc gtgtccggcc gcgtgcccgt cagctccgcg tacacgccct ggatcctccg
     3001 ccccaccgac tgccacgagt acagctcccg cacctcgcgc agcgccgtgg ccgccaggcg
     3061 ctggcgcagc ggcgcgtcgt ccatcaggcg gcccatcgcc tccgccagcg cgacgctgtc
     3121 cttcggcggc accagcagcg cgtccctccc gtcctccagg cagtccacca ctcccaccgc
     3181 ccgcgtcgac acgatgggca gcccggacgc catcgcctcc agcagcgtgt tggagaaccc
     3241 ttcggagtac gtgggtgaca cgaacaggtc cccgctccgg tacaggtccg gcgcctccgc
     3301 gtaggaggac acgcccgtca gctccaccac gtcctggagc ccgccctctt ccacccgcgc
     3361 gcgcacggcg gccacgtccg gcccaatccc agacacccgc agcttgaacc tccgcccgtc
     3421 cgccaccagt cgccgggccg cgtccagcag ctccatcacg cctttccgcg cgtccacccg
     3481 gccgtggtac agccacaccg gcgggtcccg cggctgcccc agcgccgact cattccgggg
     3541 atggaagcgc tgcgtgtccg tggcgccggg gacgatggtg aaccgctcca gcggcgtccc
     3601 gtggtgcccg cgcacctcct cggcgaagga cctgctccca atcagcagcg cccccgcgtg
     3661 gcccagcacc gcgagcatcg cctgcttgtg cgtcccgcag cacgtgccca cccagtgccc
     3721 gtcccctccc tggatggaga cgacgttggg caaccccagg cgccgggccg cctccagcgc
     3781 cgccaggccg caggggtagc catactgcgc atggatgagg tcgaacggcc ggcgccggtg
     3841 ctcgcggacc accgtgtcca ccatcacctc caggtcctgc tcgaagctcg cgggctggcc
     3901 ctggttgatg cgctgctctc cgagcgactc gcagcccagc acctgcgcgc ccggaatccc
     3961 cggcggcggt ccgcccccgt acaccgccat gcccgcgggg tcgttgcggt actggctcac
     4021 catcgtcacg tcatgtccgc tggccaccag ctcccgcacc aggttcagcg catagacgct
     4081 catccccgag atggccggga agaagcgccg ggagatgaag cagatccgca gccggttcac
     4141 gctgttcatg ccgccacctc cgcgcccgtc ggcaggcgcg ccctcagcca ctccagcgcc
     4201 tggggcacca tgtcgtgcgc ccggtgcgcg tcccgggaca gctccacgca caccagccgc
     4261 ccatagcccg cgcgcgtcag ctcgcgcagg accgacggca cgtccacgtc cccttcgcca
     4321 aacggcaggt gctcatgcac gccgcgcttc atgtcctcca gcgccaccgt gcccagcacc
     4381 ggcgcgaact cgcgcaccgc ctccgcgggc gtccgctcct gcgtcaccag caggtgtccc
     4441 acgtccaatg ccagacgcac tccgggcacc cgcgcgtgca gtcgccgcca gccgtccacc
     4501 gtctccacca gcatccccgg ctcgggctcc accgccagca ccacgccgcg cgccgccgcg
     4561 tactccgcca gccgcgccac cccttcctcg agccacggcc acgcgagcgc ctccgtcacg
     4621 cccggaaggg gcacgcccgc ccagaaggac accgcctctc cccggcacgt cccgcacacg
     4681 tccaccgcgc gcttcaggaa ctccagccgt cgcgcacgtc cttccgcgtc cggcgtgatg
     4741 agcgtgggct cgtgcttccg gcgtggatcc agcaggaagc gcgcccccgt ctccaccacc
     4801 agccccaggc ccagccgctc cagccgcgtg gccagctgct ccgtgcgctg cccgaagtcc
     4861 ggagcgaacg ggtcgaagtg atggtggtcc agcgtcaggg ccactccgtc atagccgctg
     4921 tccgcgatga ggctcagcgc gtcctcgaag cggtgatggg acacgccgtt ggtgttgtag
     4981 gcgaagcgca gggccatgac tcattgcccc ccgaagcggc ggctcgagag caccgccggc
     5041 accgcgtgcc ggtccggctc ccggtagagc gcgtacagcc gccccacgcg cgcctccgcc
     5101 agccgcacgt tgaaccaggc cggaccgccc agccgcagct cggcctccgg cgtcagccgc
     5161 accggctcgg ctcccggcgg cggcgctccc aggcccacca gcggatccct ccgctccagc
     5221 agccgcgcca cgttgcgctc gcccacgcgg ttgtacgtca tcgtctccac ctccaggccc
     5281 ggcaccagcg ccttcaccac gtgcacctgc cgccccggcg gcgacaggtc cgcgaccagg
     5341 atgtcgaggc ccgacgcgtg cagctgccgc accacgtggt cgcaccgcag ggacgggtcc
     5401 tccagcgcgg gcgcgcgagg gaggtcctcg aagcgcacct tctggcggac catcagcgtg
     5461 cacgcggtca gctcgctcag ctcgggggcc gacagccgcg cccactccac catcgcgttc
     5521 agcgcgcgcg gctcctcggc ctccaggtcc accatgggca cgaagcggtc catgtattcc
     5581 tggggcgcga cgcgggccac ctcgctgagc ggtccgtgca tgaacgcctt gcgcgcgcgc
     5641 gagcaggcgt actccagcag cgccttgcgc agcgcccggt cgcggtccgg gtcggccgcc
     5701 tcgccgcagg ccgtcaccat cagcggctgg ttccccacgg acacgtcccg gcccacgacg
     5761 tacaggttca ccaggccgaa gtccgtgcag gccagcttcg ccatcacctc cacgcccgcg
     5821 cgccggtagc gctccagcag ctcgcgcacg tccggcggca ggtccgcgcc ctccaggtcc
     5881 agcgccacgc cctggtccat ggcgcggtac accagcccgt tgccgtcgcg ctggaggagc
     5941 tccagcaggc cgtgcgccag cgcctggggc cgtgtcagcc ccgcgccctg gccgttggtg
     6001 atgggcgtga tgagcggagc gcgcccctgc aactgcccgg gatggatggc gacgtactcc
     6061 tccggcacca gcacccgctc gcccgtggac agccgctgca tctccaccca gatgagcggc
     6121 atttccggct gatacggact gcccgcgggc agccccagcg tgagcggatc cgccaccgcc
     6181 gtggggcctc gcgcgcgcac cagcgccgcg tagctcccgt gcacgcgggg catgcgccgc
     6241 atggcgagct cgctgaaggt ctcctccacc agctcgccca gcgcgcccac ctgcgcctcc
     6301 tcctccgtgc ggccatagcc gttgccgtgc agcaccgcgc ccgcgtcgtt ccccaggcga
     6361 aggctggcgg aggccaccgg aatccccagc cggtcgatgg ggtcgatgcg gaacagctga
     6421 agggcgccgg tggacatggc gcggtgatag gcatcgaggg ctcgggcaac gctcatgaca
     6481 gctcctccag gggaagggga cgggtgtgcg gcatgcccgc gagcagccgg tcgaacagcg
     6541 ccagcgcgtg ctcgcgggtg acgccgcgcg cgaactcgaa cggggtgaag acgttctcga
     6601 acgggagcgc ttcgagcacc gcgcggtagc ggcgcagctc gccgtggctc agcggaatcg
     6661 cgtagtggaa gcccttgtgg cacgacagcg ccgtgggcct gcctcgcgcg tccaggtcca
     6721 ccttcagcgc gtctccgcag aacagcgagc gggtccgcgc gtcgtacagc accatgtggc
     6781 cctcgtagtg gccccccacg cagtgcagcg tcaggtccgg cgccagtgca tgttcgtcat
     6841 ccaccggcca gcgcacctgg aaggccttgc tgtagggcac cgcctcccgg tgcagcacga
     6901 gcagcgggtc gaagtgctcc tgcaactgcc acagcgcccc gaagccgtgc gggtgcgacg
     6961 ccgacagcac gtggaggccg cccatcacct cgatgaactc cagcgcgggc cgcgagtacc
     7021 agggcgcgcc ctcgaagccc acgttgcctt cgggcctgcg cagcagccac cccgtggagc
     7081 ccaggccgaa gggcggcgtg caggtgaagc cccagatgcc gggcagcgcc tcatgccagc
     7141 gcgtcccgag ccgctccgac acctggcccg cggactggaa ctcccagccc tcgcgcggca
     7201 gcgcgttgcg cacgtccagg caggtggggc accccatggg cttctcccgg gggaagcccg
     7261 gctgccacgt gccgcagtgg gtgcaggcgt agcgggtcag tggcatggcg gaaacgccct
     7321 tccgtcccgc gtcgcctgcg acagcagccc cacgagccgc acgtcacgct cgggcgcgta
     7381 gggctgcgga cggccctcca gcacgcacgc ggagaaggac tccacctgat ggaggaaggg
     7441 gctcgggtcc tcctccggcg ggaggatgat ccgcgtctcc ccgcccgtct tcgcgtcggt
     7501 gagggtcagg gtgccgccgg gcgtctgccc cattgtcttg tacgccagcg cgcgcgcctt
     7561 cgtgccgatg agctccagcg tgcgccgggg gtacgtgtcc gggcagttgt aggccacctg
     7621 caacaggccc agcgtgccgc tcttgaactg gcccatcagc accgcgccgt catccacggc
     7681 gtaggtgtgc acgcgccgct gcgtcagggc gctgagtgac gcccactcgt cgcccagcac
     7741 cacctccagc aggtccaggc cgtgcggcgc caggtcgatc atcgcgccac cgccggcctg
     7801 ctccgggtcg atgcgccagt tgtccggcgc ccagtcctcg ggcagccagc aggcgtagtg
     7861 gatgcgcgcc tgggtgatgg tgcccagcgc gccctccttc accagcgtgc gcagccgccg
     7921 gtgcgccgcg tggtggcgct ggtcgaacgc ggtggcgtag tgcacgcccg cgcgctggca
     7981 cgcggacacc atgcgcaggc cgtcgcgcgc cgtgatggcc atgggcttct cacacagcac
     8041 gtgcttgccc gccgccgcgc aggcctcggt catcgccacg tgcaggtgat tgggcgtggc
     8101 gatgtagacg gcctgcacgt ccttgtccgc cagcaccgac cccagcgcgg tgtagcgatt
     8161 cacgccgtcc gcgcggatgc gcatcaacgc ctcggcgctc acgtcgcaca gcgccaccag
     8221 ccgcgcgttg ctcgcgccct ggagcgcggg gatgacgtag tcccgcgcca cccagccaca
     8281 gcccaccacc gcccagccca ggcttcgggt ccccggcttc gcctcgaaca tgatcaccgc
     8341 ctccgcaaag aaggttcagg aaccgccgcc cgctcagggc cacaccgccg cttcgcgcag
     8401 gacgtcgttg ccgaaggtct ggagcggccc ggggtggatg agctccaggt cctccatggc
     8461 ctggcgcagg gtgaagcccg ccgcgcgggc gtgcgcctcc agctccagca cccggtccag
     8521 cgcgtccgcg gcgttgaagg cgcgcgcctc cgccgagtcc acgttgccgg tgagcgtcag
     8581 cgcctgccgc acgacgcccg ccagccgctc cggcagcgcg gccagggcct cctccgtgag
     8641 gcgcttcatg gcgggctcca gcaggtcgcc cagcagcgcc tctccgccgt agcccgcgtc
     8701 cggcagcgcc gcgttgaaga ggtgatgcga gagccccgcc acgaagggca gggccgcaga
     8761 cgcgccgaag cgcggcgcga cgagcaccgc gcccaccgcc accgcgtagc agtggtccgc
     8821 gtggccctcc tgcggcgaga cgaggatgcg cggccggccg gggaacgtgg ccccggcgcg
     8881 gggctggcgc accagccgct ccacgaacca cggcggtggc gcgtgacatg cctcctccgg
     8941 cgtctgggcg agcgccacgg acagccgctc cttcaggcgg gcatccagcg gcgcggccac
     9001 cgcctccagg ccccggtgca gcacccgcgc cgcgtccagc ggggacagcc ccgcccgctg
     9061 gaggacgtcc atgtccaggc cgcccagccg caccgccgcc accgcgcgcg ccgtctcctg
     9121 tagcgccacg gtgcgcggag ccatgccgga ggccagcgag gcccacgcgc ggcggaagcc
     9181 gtgcgccgcc aggccgtccg ggtggtccgg ggtgcggatg cgcttgaggt ccatcagctc
     9241 cgacaggagc ggacggagct ccgccagacg gccggcggcc gccgtgtcat gcgcgggtgc
     9301 gttcatggcg ctcagcccct gatgcgttcc gggaggcgct ccacctgctg cgcgcggccc
     9361 ctggagagcc acgccatcag gcggcgctgc tgctcgggca tggcgtgctc cacgggctgg
     9421 ccgtcctgcg acatgggggc cttgaagaag ccgcccagcg cgtcgatgac gccgcactct
     9481 ccccggcgct tcgccaggtc cagcgtgcgc gcgagctcca cgacgagcgg cgcggcgagg
     9541 atggagtcct tgcagaggaa gttgagcttc agctgcatgg gctgcccgag gaagcccgtc
     9601 acgtcgatgt tgtcccaggc ctccttgttg tccccgcgcg ggcggtagta ctggatggag
     9661 acgacgtggt cctggacctt gtagccgagg atgctgtcca gggcggcgcc cttggtgtcg
     9721 agcttgttct gccgcgacgc cgggtcgttg agggcctccc cgtcgcggtt gcccaggatg
     9781 ttggtggagt accagccgtc cacgtgcagc gcgcggtcgc gcagcgcggg cgcgagcacc
     9841 gtcttgagca gcgtctgccc cgtcttgccg tccttgccgg cgacgggcgc gccggtgcgc
     9901 ttcgcgagca tgagcaacgc gggcacgtcc gcggcgacgc tgggcgtgaa gttggcgaac
     9961 gggatgccgt ccccgatggc cgcgtacgca tagagcatcg cggggccgat gtccgggtgg
    10021 ttcgcgtcca gggccttctc gaaggactcc ggcgtggcga actccgggcg ggtgaggtcc
    10081 acggccttct ccgtggaggc caggttcacc accaccacgc ggtccagctg ctcggcctgc
    10141 ttgaagcggg tgaggtcctc gcggatggcg cgcacctgtt cgcgcagcgt gttcgcgttc
    10201 ttcgtcgcgg tgccgacgac gttcttgcag aacctcgcgt tggaggccgc gggccacggc
    10261 cgcatgcggc ccagcgtggg cgccaccgcc tccagctgcg cctccgtcag caccgcgtgg
    10321 ttgcgcgccg cgcgcgccag gtcatcctcg aagaggtccc agccgccgaa cgtcagcgcg
    10381 ccgtactcca ccagccccat gcccttcgcg tcggccaggg gcaggccgcg cgtgtccacc
    10441 cgtccccgcc gcaagagctc catgcccgcg accgccgtgg tcgccaccgc gccgcccaag
    10501 cccaccaccg ccacgcccag ccgctcatcg cccgccatgt gtgaagcccc tccccaaccg
    10561 ttgtgccccg tgccctcgcg gcgacactcc ggtggctccc cgaggcgaca ggagccccgg
    10621 ggcttggggg cgcttgccgc gcccactcgc tgcgagagtt tcgggaaagt cgccacatgg
    10681 ccgggggctt tcaactcagc ccctggccgg tgcggactgt ccgctggtgg aggcggggct
    10741 cagacgtggc gcagcttcca gccgccgcgc cggaagaggg cggcgctggc caggccgagc
    10801 gccccgtagg tgacggcgat ggcgatgaac gcgcccgccg gccccaggcc cagcggatgg
    10861 gagaggaccc acgcgagcgg cagctggagg ccccaggaga agagggcgtt caccacggtg
    10921 ggggtggtgg tgtcccccgc gccgttgaag gcgtggggca gcaccgtgac gaaggcatag
    10981 agggccaggc tgcagctgac gatgcgcagg cagcgcgcgg cgtgcaggcc cacctccgac
    11041 tcggcggtga agcggtggat gagcggctcc gcgaagagga ggaagcccac cgccaccacg
    11101 cccaggaacg cgagcgtgta gaagctggcg cgccacgcgg ccttctccgc gcggtcggtg
    11161 tcgcccgcgc ccaggctctg gcccaccagc gtgcccgccg cgtgggacag gccccacgac
    11221 ggctgctgca cgaagagcag gatgcgcatg gccagcgtgt agccggcgag cgccgtgctg
    11281 ccgaaggtgg ccacgatgcg catcagcacc agccagctgg acatgcccag cagcgactgg
    11341 agcgtggcgc cgcccgacag cttcaggagc gagcgcagcg tggccccctc cacgcgcagg
    11401 tggcggcggc gcagctccag gcggccgcgg cccttgagca ggcgatacac ctgatacatc
    11461 acgccggtgg agcgcc
//
