LOCUS       NZ_CP082820            10867 bp    DNA     circular CON 26-OCT-2024
DEFINITION  Streptococcus pneumoniae strain SCAID PHRX1-2021 chromosome,
            complete genome.
ACCESSION   NZ_CP082820
VERSION     NZ_CP082820.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2098200)
  AUTHORS   Korotetskiy,I., Kerimzhanova,B., Jumagaziyeva,A., Myrzabayeva,A.,
            Shilov,S., Kuznetsova,T., Iskakbayeva,Z., Myrzagali,A., Reva,O.,
            Satylgankyzy,G., Korotetskaya,N., Zubenko,N. and Ivanova,L.
  TITLE     Clinical isolates genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2098200)
  AUTHORS   Korotetskiy,I., Kerimzhanova,B., Jumagaziyeva,A., Myrzabayeva,A.,
            Shilov,S., Kuznetsova,T., Iskakbayeva,Z., Myrzagali,A., Reva,O.,
            Satylgankyzy,G., Korotetskaya,N., Zubenko,N. and Ivanova,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2021) Virology and microbiology, Scientific Center
            for Anti-infectious Drugs (SCAID), 75A Al Farabi Ave., Almaty
            050060, Kazakhstan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: LR216047
            Genome Coverage                   :: 386x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019915285.1-RS_2024_10_26
            Annotation Date                   :: 10/26/2024 03:31:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,134
            CDSs (total)                      :: 2,061
            Genes (coding)                    :: 1,894
            CDSs (with protein)               :: 1,894
            Genes (RNA)                       :: 73
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 167
            CDSs (without protein)            :: 167
            Pseudo Genes (ambiguous residues) :: 0 of 167
            Pseudo Genes (frameshifted)       :: 87 of 167
            Pseudo Genes (incomplete)         :: 79 of 167
            Pseudo Genes (internal stop)      :: 50 of 167
            Pseudo Genes (multiple problems)  :: 42 of 167
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:29:32
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 532545
            Orig. end                         :: 543412
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082820.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10867
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10867
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10867
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5867](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5867
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1004..1132)
                     /gene="blpC"
                     /locus_tag="K8B76_RS02575"
                     /old_locus_tag="K8B76_02575"
     CDS             complement(1004..1132)
                     /codon_start=1
                     /gene="blpC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /locus_tag="K8B76_RS02575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02575"
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="WP_000358815.1"
                     /transl_table=11
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(1189..2550)
                     /locus_tag="K8B76_RS02580"
                     /old_locus_tag="K8B76_02580"
     CDS             complement(1189..2550)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /locus_tag="K8B76_RS02580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02580"
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_001069072.1"
                     /transl_table=11
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKEI
                     TVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTALAT
                     QLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFINFTKQSHDIELGITKTNTEV
                     SNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRYLVAS
                     QGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVLRTQFL
                     QTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRIPTGTEI
                     AQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQSIDQTPT
                     RTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILTHSD"
     gene            complement(2561..4719)
                     /gene="blpA"
                     /locus_tag="K8B76_RS02585"
                     /old_locus_tag="K8B76_02585"
                     /pseudo=""
     CDS             complement(2561..4719)
                     /codon_start=1
                     /gene="blpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /locus_tag="K8B76_RS02585"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02585"
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTSY"
     gene            5001..5255
                     /gene="blpM"
                     /locus_tag="K8B76_RS02590"
                     /old_locus_tag="K8B76_02590"
     CDS             5001..5255
                     /codon_start=1
                     /gene="blpM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /locus_tag="K8B76_RS02590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02590"
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="WP_001093256.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.1e-18,
                     bitscore: 64.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKMMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGLG
                     TAICAASGVGAPFMGACGYIGAKFGVALWAGVTGATGGF"
     gene            5271..5474
                     /gene="blpN"
                     /locus_tag="K8B76_RS02595"
                     /old_locus_tag="K8B76_02595"
     CDS             5271..5474
                     /codon_start=1
                     /gene="blpN"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /locus_tag="K8B76_RS02595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02595"
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="WP_001099490.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.4e-12,
                     bitscore: 44.0, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGPW
                     GAAIGGIGGAVVCGYLAYTATS"
     misc_feature    complement(5695..5710)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.42"
                     /tool="antismash"
     gene            5718..5867
                     /gene="blpO"
                     /locus_tag="K8B76_RS02600"
                     /old_locus_tag="K8B76_02600"
     CDS             5718..5867
                     /codon_start=1
                     /gene="blpO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /locus_tag="K8B76_RS02600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02600"
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="WP_001809846.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 3.5e-10,
                     bitscore: 37.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDGC
                     ATTV"
     gene            <5903..5968
                     /locus_tag="K8B76_RS11080"
                     /pseudo=""
     CDS             <5903..5968
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863320.1"
                     /locus_tag="K8B76_RS11080"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ComC/BlpC family peptide pheromone/bacteriocin"
                     /pseudo=""
                     /transl_table=11
                     /translation="VTGAAGGAILGGVAYAATCWW"
     gene            5971..6090
                     /locus_tag="K8B76_RS02605"
                     /old_locus_tag="K8B76_02605"
     CDS             5971..6090
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /locus_tag="K8B76_RS02605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02605"
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="WP_000346296.1"
                     /transl_table=11
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     misc_feature    complement(6226..6239)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.08"
                     /tool="antismash"
     gene            6388..6552
                     /locus_tag="K8B76_RS02610"
                     /old_locus_tag="K8B76_02610"
     CDS             6388..6552
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727116.1"
                     /locus_tag="K8B76_RS02610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02610"
                     /product="hypothetical protein"
                     /protein_id="WP_000727117.1"
                     /transl_table=11
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYFA
                     INKKKAEKK"
     gene            6614..6952
                     /locus_tag="K8B76_RS02615"
                     /old_locus_tag="K8B76_02615"
     CDS             6614..6952
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826248.1"
                     /locus_tag="K8B76_RS02615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02615"
                     /product="immunity protein"
                     /protein_id="WP_088804618.1"
                     /transl_table=11
                     /translation="MLVSTYLFLSRQFLNFSLVFQFLLVVINLTSILVTVYLTREMRIR
                     KFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAAESYQQIIAYIGAVLCLLIMLLLI
                     WGLKYYKK"
     gene            7581..7964
                     /locus_tag="K8B76_RS02620"
                     /old_locus_tag="K8B76_02620"
     CDS             7581..7964
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /locus_tag="K8B76_RS02620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02620"
                     /product="hypothetical protein"
                     /protein_id="WP_000877381.1"
                     /transl_table=11
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGVF
                     LLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHIFI
                     VFNALMYLLSSSQVSLKKNKLSS"
     gene            8016..8705
                     /locus_tag="K8B76_RS02625"
                     /old_locus_tag="K8B76_02625"
     CDS             8016..8705
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /locus_tag="K8B76_RS02625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02625"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000760520.1"
                     /sec_met_domain="Abi (E-value: 1.1e-09, bitscore: 35.5,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFVW
                     IQNILSLLFSGVMIWILVKTGHGYLFRIPRKKWLWYSILTVLVLVLQISFNVQIAKHVQ
                     STAEGWAVLIGYSGTNFAELGIYVTLFFLTPLMEELIYRGLLQHAFFKHSRFGLDLFLP
                     SILFALPHFSSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFPFLLT
                     FLHRVLG"
     gene            8747..8995
                     /gene="blpZ"
                     /locus_tag="K8B76_RS02630"
                     /old_locus_tag="K8B76_02630"
     CDS             8747..8995
                     /codon_start=1
                     /gene="blpZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /locus_tag="K8B76_RS02630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02630"
                     /product="immunity protein BlpZ"
                     /protein_id="WP_000276501.1"
                     /transl_table=11
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFNF
                     LNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKNDV"
     gene            9025..9636
                     /locus_tag="K8B76_RS02635"
                     /old_locus_tag="K8B76_02635"
     CDS             9025..9636
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /locus_tag="K8B76_RS02635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02635"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000394036.1"
                     /sec_met_domain="Abi (E-value: 1.7e-19, bitscore: 67.0,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSLL
                     VTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHLAE
                     VSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSNVPS
                     FIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            9797..10591
                     /gene="ccrZ"
                     /locus_tag="K8B76_RS02640"
                     /old_locus_tag="K8B76_02640"
     CDS             9797..10591
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /GO_function="GO:0004747 - ribokinase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /GO_function="GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /GO_process="GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ccrZ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /locus_tag="K8B76_RS02640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K8B76_02640"
                     /product="cell cycle regulator CcrZ"
                     /protein_id="WP_000363002.1"
                     /sec_met_domain="APH (E-value: 4.1e-18, bitscore: 63.1,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGLA
                     REQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQLS
                     RLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDVRHS
                     NWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTVLSKL
                     YWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     misc_feature    complement(10754..10768)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 17.11"
                     /tool="antismash"
ORIGIN
        1 gcttttttga aatggttgga ctacgatttt actagcttga gaaaggagat tctcgataaa
       61 gcctttcaaa agtccctgac tcagattaac tggataatgg gtggctacta tctagtcatg
      121 gaaagtctgt ctttttttga atatgaacaa agtattcaat caaagactgt tcgccatctc
      181 atcctagtct tttacctact cttttttatg ggggttatca agaaattgga tacctatttg
      241 aaggaaaaac tctatgagag attggaacaa gagcaggccc tgcgctatag agatatggaa
      301 cgctatagtc ggcatataga ggagctttac aaggaagtac ggagctttcg tcatgattat
      361 accaacctct tgactagctt acgtctgggc attgaagagg aggatatgga gcagataaaa
      421 gaggtctatg gctccgtctt aaaggattcc agtcagaaat tgcagaacaa taaatatgac
      481 ctgggcagat tggtaaatat tcgtgataaa gccctcaaaa gtcttctagc agggaaattt
      541 ttaaaagcca gagataagaa cattgtcttt aatgtcgaag ttcctgagga gattcaggtc
      601 gaggggatga gtctacttga ttttctaacc attgtgtcta tcctttgtga caatgctatt
      661 gaagccagtg tggaagctag tcaacctcat gtttcaatcg cctttttaaa aaatggagca
      721 caggagacct ttatcatcga aaactccatc aaagaagagg gcatcgatat ttctgaaatc
      781 ttctcctttg gagccagttc taaaggggag gagagaggag ttggtctcca taccgtcatg
      841 aagattgtgg aaagccatcc taataccagt ctaaatacca cctgtcaaaa tcaagtcttt
      901 cgtcaggtac ttactgtgat acatacagaa tgatgaaaaa taagaccgag agtacttgtt
      961 tctcggtctt atttttttat agttgtattg gatgatgaag ttcttatgtg atgtaatgag
     1021 catatctatt aatgttatat aataaatctt cccacaatcc tccacctgta atttggttga
     1081 gttcggtagt tgttagttct tgaaatgaag ttaggttttg tttcttatcc atgttatgat
     1141 tctccttttt gataagataa taaatagtta tagagtgtta tctgaaaatt aatcagaatg
     1201 tgttaaaatt ttatctttga aataatcaaa atatgttttc tttgtagtta cactagtgac
     1261 gcgaccttgt aagccatatt ggatgagttt actatcctcg ttagatagtt ttgcaagagc
     1321 ggttaattta aagagattgc cttgctctgt tctggtagga gtttgatcaa tactctgaag
     1381 ttggccgatg atggtggtgc cgtgatttcc aatcttctcc agttttaatc ttacagtttg
     1441 tcctttatct agtaagggga gatagtcaga tgatacgtag taagtgatta gtacttctct
     1501 tgtatctgtg atgacaggga atatttgagc aatttctgta ccagttggaa ttctattttt
     1561 accttcaaat tcgctgttca gatgaacgat acctttactt ggagcggtta aggtattatt
     1621 ttctaagcgc tgtgtcgctt gatctagttg tacttttaat tctgttaatt gattctccac
     1681 agttagttgt tgctgtgagg ctgtctgtaa aaactgagtg cggagtactt caattttggt
     1741 tgctaaactg ttatcataag ttgctacact tccgatacca gcttgctgaa ttttgaggct
     1801 tgcgatagat gattcaagac ctgcaatact ttgattaatt tgagataaaa atggctcctc
     1861 tgcagttcct tgtgtttgtc cttgtgaggc tacaagataa cgattcaaaa ttgactggtg
     1921 cggattgcca gttggtaagc gtgctctgtt atttatgata gcatctctca actcttgata
     1981 ttctccaatt tgttgttgaa cttttgtaat ttcttgttca atagctgatg aactgctatt
     2041 ggaaagatta gcttgatttg aaacttcggt gttagtcttt gtgataccca gttcaatatc
     2101 atgggattgt ttagtaaaat ttataaaggt attatggtag ccaaattcat cctcgccaga
     2161 aaaaagatca gtcgcttttt ctaagctttg tttcaaaatt ccaagtcctt ctttttgctt
     2221 ctcaagtctt tgtaattgag ttgctaaggc agttttctga ctttcttcca ttgtttcaga
     2281 gtatttgatg agtaagtccc ctttttcaac tacttgattt gccactaaat gattagctag
     2341 gataggatta tcactggttg actgaatgga ggcaatgaca cttgtagggg cgatttctcc
     2401 ttgggaagta acagtaattt cttttgtggc aacaagggag aaaatcaaga tgaaagtaaa
     2461 tagtaatgaa agaggtataa ttaacactgt cgcatagtta tggtaacgtc tctgataaaa
     2521 ctcgacgctt ctaaaaagat taggattcat gacattctcc ttatttattg aatagatgat
     2581 ggtagaaacc ttgcgcctgc attaactctt ggtgactacc aacttcaatg attttcccct
     2641 ggtcaagaac aatgacacgg ttggttcgtt cggctatact gagacgatgg gctacaaaga
     2701 gaatggtttt atcagttaga gacataagat tatctataac ctttttctca gtcaagacat
     2761 caagaccgct agtagcttcg tctagtatta aaacaggaga tttagttaaa agagcacgag
     2821 cgagggctat tcgttgcttt tgtcctcctg atagaccagc tccatcagag agctgagttt
     2881 gatagcccat aggcattctt tcaatgtctt gacgaatttc agctaattca caagctctta
     2941 gaatatcttc ttggctaatc atatgattac cgcccaaagt taagttttcc aagatagagc
     3001 cattaaagat ataggcttgt tgtggtaggt aattaatatg acggcgcaag acttttttat
     3061 caatgttttt aatatcctga tgattgatgg aaatatgtcc tttgtagggt tcaaagaaat
     3121 tgacaatcat tttggctaaa gttgttttac cagaaccact aactccaact aggctaacct
     3181 tatctccttg tttaatcgtg agattaatat ctgttaaggt atctcgtcca aaaccatact
     3241 tataagaaag gtcatcaaat tcaatatcgc ccatcaaaaa atgtgaatga acagggtttt
     3301 cttgaacttg aaattcagat tcgactagat agacttcgtt caaacggtta ttagcgacct
     3361 tcgcagattg gagtttggtt tggaggttga taatattttc cataggagtt gtaaagtaag
     3421 aaaaaagtgt gttaaaggta atcagctgac cgatagaaat tttacttgac atgactaatt
     3481 gagcgccaaa ccataggata aggatattca gaactaattt tgttccctgc tttaaactcg
     3541 tttgtaaaat agaatattta ctgagcttaa aggatttttc caaataatct acaaattcgc
     3601 tgtctatatt ttgatagcga ttttcttcac tcgtgagcga ctttatagtt tcaatcccgt
     3661 tgatatcttc gataatggca gagctaacca tagaattact ttgcatgaca tcatggttca
     3721 ttttttcgaa aggtttcata aaagaaaaga tgatgaacat gtatatagga atggaaataa
     3781 gagaaagaag gaagagatta gggttttgtg ccagtaagac gcctcctaca agaatcagaa
     3841 tagaaacatc cagaaaaaga gaaagaatgg tagaagccaa ggcatctata atagagttag
     3901 catctgtgaa tcgtgaaatg atttctcctg tacgacgtgt cgcaaagaaa gacatgggaa
     3961 gttcaaaaat atggcgaata taggataaaa tcacatcaat acttaatctc tgactcagaa
     4021 cggttaggag ataatctctg gagaagctca tgacttgttg gaggatatag gtgataacca
     4081 gaccaactga gatgattcct aaagttgatt tcatctgatt tggaatgtat tcatccaaga
     4141 ttccttgtag atagtaagaa ccacctatat tgataatagt gaccaataag cttgagagaa
     4201 caatgtaagc aatgagagat ttttgtttga aaatcagagg aaggaagctt gcttagtaga
     4261 ccattctttt tatctttatg gggttgatag ctgggtttgg tagctagaaa aatagctact
     4321 ccagtccatt catagaaaaa gcgttctttt gacattttag tgatttttac agaagggtca
     4381 ggatcaccaa taatcagata gtctttcttt gtttgataga caacatagta atgttggagt
     4441 tttccttctt tgttaacgtg aacgataaat ggatagggga catcactcat gtcaaagagc
     4501 gttttatctg cttgaacagg tcttgtttca aagcccattt catcagcggc ttttacaatg
     4561 ccaagagcag tcgtcccttc tttattggtc tttgcaagtt ctctcaagtg agctagagaa
     4621 aaatctgaac catagaattt agcaatcgag gctaaggcag cgacaccaca gtctctcgca
     4681 tctatttgag gaacaaatgt acgtttaata agaagtcatt ggcaattcct ttcatatagt
     4741 ggataggtct attatgtcat aaaactaagt tcatatttta tgaaatcttg aatagtcatt
     4801 aaaacttcct gaatggtaaa aaagtgatta gaaattattt tttttaaaca tttagaggtg
     4861 gcttgaaata aaaaagctaa ttcaagacgt ttcgatgaca attcaagatc tggatgaaaa
     4921 attttaaaaa acgatgatat actaaactta tcaaagttac aacaagacaa aaataaaaaa
     4981 agaaaaaagg agtatttgtc atgaatacaa aaatgatgga acaatttcat gaaatggata
     5041 taactatgtt atctagtatt gaaggaggta agaataattg gcaaactaat gtcttagaag
     5101 gtggtggtgc tgcttttggt ggctgggggt tagggacagc tatttgtgct gcgagtggtg
     5161 ttggagcgcc atttatggga gcatgtggat acataggagc taaatttggt gtggctcttt
     5221 gggcaggagt aacaggggca acgggtggat tttaacaaaa ggagacttgt atgaatactt
     5281 attgtaatat aaatgaaaca atgctgagtg aggtttacgg aggtaattcc ggaggagcag
     5341 ctgtagttgc tgctttaggt tgtgcagcgg gtggagtgaa atatgggaaa attctaggac
     5401 catggggcgc tgcaatagga ggaattggag gagcagtggt ttgtggatat ttagcctata
     5461 ccgctacatc ataaaaatga aagctaaatt ttatgaaggc tatattcttt attattctct
     5521 tcgcttttca aacctatctt atttatctat ccataagtat ctctgataaa aaacaaaaga
     5581 ctattgaatt aacatcattg aattgttttg tcatattatt tctaatctat gataaattaa
     5641 tttttctctt tattgcttat gtttttttga taatttttat attaaacttg ttctgtaact
     5701 gaaaagtgac atactatatg aatacaaaaa tgatgtcaca attttctgtt atggataatg
     5761 aaatgcttgc ttgcgttgaa ggtggagata ttgattgggg aagaaaaatt agttgtgcag
     5821 caggggttgc atatggcgca attgatgggt gtgcaacaac ggtttgacat ttctattggg
     5881 accatttgct ataggaatag gtgtaactgg tgctgcaggt ggagctatac ttggaggtgt
     5941 agcctatgca gcaacatgtt ggtggtaatt atggatttta aaagttttat tattggttta
     6001 gtagttggta tatttggtcc ttatatggat gacttaatta gaaaaatatt ttcaaaatct
     6061 tccaaaaagg atacggatag tactctctaa aaaataaatt tctcccctgt atttgctagc
     6121 ttaatttaca ggggagtttc tttaatattg tttcgcctgt caaaatgata tgtgatagca
     6181 ggatgaaaag tctacaaaca agttggtatc aatacaatat aaagaggaaa gtatgttttt
     6241 catgcttggt ttttttaatt tttacaattc aagaggtttt gatgaccatt tatgatttga
     6301 gtgatccaag gtcaaaataa gtgctatact agcagtgtaa atgttcttgc tcaactaaga
     6361 taaatcgcac atttaggagg aaatcttatg aagaaaaaaa tactgattat tttcgttctg
     6421 tatctaatca tgtccatctt tctatatccg cttagggaga gtgcttggta tcagctattt
     6481 tataccatag cctatgtgat tgcagttatg atctattttg ctatcaataa aaagaaagca
     6541 gaaaagaaat gaaaactttt cttgctaaaa acggaacatc ttccttgcga gattgttctt
     6601 aggtcagttg cccttgcttg tctctactta tctatttcta tctcgtcagt ttttaaattt
     6661 ttccttagtt tttcaatttc ttttagtggt tattaattta acttctattt tggtcactgt
     6721 ttacctcact agggaaatga ggataagaaa gtttgaagat gatgatttgg ttagtcctag
     6781 aaccaatcaa ctcatgttta tcggcttgac aggttttatg tctattatat gtttgtatag
     6841 aggtatcaca gcagcagaat cctatcaaca aataatcgct tatattggcg cggttctctg
     6901 cttgcttatc atgcttttgc tcatttgggg tttgaagtat tataaaaagt aggggctaat
     6961 gaagtttttg caatttagga ggtttcgatg acaattcaag atttggatga aaaattttaa
     7021 aaaacaatga tatactaaac ttgtcaaagt tgcaacgggt gctgctacat tatgttaaaa
     7081 atcgctcttg attgataagc tggatttgtt aggaaaggag taaataatta tggataagaa
     7141 aaagatagtt tcgactataa tatgtatagt atttcttgta gtgtcagtag ataatttttt
     7201 tagagactta accccgttac tgttcatttt aaatattatt ggcctgtctt gcttttcagt
     7261 gctaacatac attaacataa aagagatact cttaaatata agtaaatgat gatttggtta
     7321 gtcctagaac caatcaactt atgtttatcg gcttggcaga ttttatgttt gtcatttgtt
     7381 tgtatagagg tatttcagaa acagaattct atcaacaact aatcgcttat ataggtgtat
     7441 tctctgcttg tttatcacgc ttctgctcat gtggggcttg aagtattata aaaagtagct
     7501 ttctatcctt ttctgacagg atatttgagt agaaggctct attaaattgt tagtgaaata
     7561 aatgaatgga ggtatttaat atgaaataca gattattttt tgttattttc ttgagtagtg
     7621 tgttggatat tcttttaggg acatttttac aaatctctat cgtatctatt gggtggcttg
     7681 ttctttacag tggtttgttt gaagcaggag ttttccttct tgctaataaa ggggtggcgg
     7741 taaaaatcaa ggaagtagat attcgaaatc gctttaaatt tatttttgga aaaaccttat
     7801 ggtttcaaat tcttttgctc atctttttga taatcaaact ttatcttggt ttggatgcga
     7861 ggttgatttt attctatgga catattttca ttgtctttaa tgccttaatg tatctattat
     7921 ctagtagtca ggttagcctt aaaaaaaaca aactgtcttc ttaatcttac agcagtagag
     7981 cctgacaagg tgaggtcata gtagggagga tttctatgaa aaagtatcaa cttctattca
     8041 aaataagtgc agtcttctct tacttatttt tcgtatttgg tctttctcag ctgacgctta
     8101 tcgtccaaaa ttattggcaa ttttcttccc agattggtaa tttcgtctgg attcaaaata
     8161 tcttgagttt actatttagc ggagtcatga tttggattct ggttaagaca ggccatggtt
     8221 atctctttcg cattccaaga aaaaaatggc tttggtattc gattttgaca gtattagtgc
     8281 tagtgctcca gatctctttt aacgttcaga tagctaaaca tgttcagtca actgctgaag
     8341 gttgggccgt attgatcggt tatagtggga ccaactttgc tgagctaggt atctatgtaa
     8401 ccctgttttt cttgactcca ctaatggaag agttgattta taggggatta ctgcaacacg
     8461 ccttctttaa gcattcgcga tttggccttg atttgtttct tccgtccatt ttatttgctc
     8521 ttcctcattt ttcaagccta cctagtttgt tagatatttt cgtctttgca acatttggaa
     8581 tcatctttgc cggtttgacc cgctatacca agagtattta tccatcctat gcggtgcatg
     8641 tgatcaataa tattgtagcg accttcccgt ttttgctcac ttttctacat agggtcttgg
     8701 ggtaaaaaaa caaaaggctt gcttttcagc catagaggag gtcatcatgt ataaacactt
     8761 atttttccta gattccaaaa ctttagatcg gttgacaccc tatattctag tcttggcttc
     8821 tgacaccatt gcttttaatg tttttgtgct aacctttgta tctgcggtgg tttttaattt
     8881 cctaaattcc atgctagctt taatggctat attcataggg gctggctatg tggtcggatt
     8941 ttggttgcta aaatggtttg ttttggaaag gctagaacta aagaatgacg tgtaggaagt
     9001 ctgtttgttg aggaggataa ttttatggag ttttttgata aatttcatgc cttgtgtttt
     9061 ggatttttag tactaataat tgtcattaca gttccttata cgattaacca tggggatttt
     9121 tttcaaaatg aatctgcatt gattattgta agtcttcttg taacctcgct gagtgttgct
     9181 tatgctagaa agtttgaaat gatttctttt gggatgttaa gcaagaaaca acttttgctt
     9241 ttcattgcaa tctttcttct aagtgtgctt gagacgctgg tttatattca tttcttcgct
     9301 gtttcttctg gctcaggggt ccaacacttg gcggaagtca gcagaggaat ttccctgtct
     9361 ttgattttga ctacctcagt ttttggcccc atccaggagg aactcatttt cagaggactt
     9421 cttcaaggtg cggtttttga caattcttgg ttagggcttg tgctaacttc ctctctcttt
     9481 tctttcatgc atggaccttc taatgtccct tcgtttattt tttatctact tgggggcttg
     9541 ttgctgggcc ttgcttataa aaagagccaa aacttatggg tttctactct agtccacatg
     9601 ttttacaaca gttggccact cctatattat ttataaaaat tatgaaaagg taagcagaag
     9661 acggtgctta cctttttcaa gctataatga tacccaaacc actccagagg cttttctttg
     9721 agaatcgggt tagagtggtt gacgaatagg ccaaaaacta gtagaatagt aaggaaactt
     9781 tatacggagg aaagaaatgg atttgggtga taatgagcta acactgactc ccatacctgg
     9841 gaaaagtggc aaggcttata tgggtagcta tcctgatggg aagcgcatct ttgtaaaaat
     9901 gaacacctct ccaatcctac ctggtctagc tagagaacaa attgctccac aattattatg
     9961 gagtcgtcgt ttggcagatg ggcgtgatat gtgtgctcaa gaatggttga caggcaagat
    10021 attgaccccc tatgatatga atcgtaagca aatcgtcaat attttaaccc gtcttcatcg
    10081 ctcacgtccg ttgatgacac aattgagtcg tttgggctat gccatggaaa cacctgtaga
    10141 tttactacag tcttggcaag aaacggctcc agatgctttg cgtaaaaatc attttatcag
    10201 tgaagtgatg gctgatttac gtcagactat tccaggattt agagaggacc atgcgaccat
    10261 tgtccatgga gatgtacgac atagtaattg gattgagaca gatagtggct tgatttattt
    10321 agtagattgg gattcggttc gcttgaccga tcgcatgttt gatgtggccc atatgctctg
    10381 ccattatatt tcagaacatc agtggaagga atggttgacc tactacggtt acaagtacaa
    10441 tcaaacggta ttaagtaaat tgtattggta tggtcaattg tcttatttga gtcagatttc
    10501 caagtattat atgaaccaag atttagaaaa tgtcaatcgg gagattcatg gtttgcgtca
    10561 tttccgagac aagtatggaa agagaagatg agagttagaa atcgtaaagg ggcaacagaa
    10621 ttactagagg caaatcccca gtatgtggtc ctcaatccct tggaagccaa ggcaaaatgg
    10681 cgggacttgt ttggcaatga taatcccatt catgtggaag ttggaagtgg aaagggtgcc
    10741 tttgtttcag gtatggccaa gcaaaaccct gacatcaact atatcgggat tgatattcaa
    10801 aagtctgttt tgagctacgc tttggacaag gtgcttgaag ttggagtgcc taacatcaag
    10861 ctcttgt
//
