LOCUS       NZ_JAASJP010000011     22982 bp    DNA     linear   CON 27-SEP-2024
DEFINITION  Enterococcus faecalis strain K58 scaf_11, whole genome shotgun
            sequence.
ACCESSION   NZ_JAASJP010000011
VERSION     NZ_JAASJP010000011.1
KEYWORDS    WGS; RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 86213)
  AUTHORS   Lv,Q. and Li,S.
  TITLE     Detailed information of the 97 whole-genome sequenced gut bacterial
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 86213)
  AUTHORS   Lv,Q. and Li,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2020) College of Animal Science and Technology,
            Heilongjiang Bayi Agricultural University, Liming Street, Daqing
            163319, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019731285.1-RS_2024_09_27
            Annotation Date                   :: 09/27/2024 07:58:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,794
            Genes (coding)                    :: 2,742
            CDSs (with protein)               :: 2,742
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 22 of 52
            Pseudo Genes (incomplete)         :: 35 of 52
            Pseudo Genes (internal stop)      :: 13 of 52
            Pseudo Genes (multiple problems)  :: 14 of 52
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:25:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 37875
            Orig. end                         :: 60857
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAASJP010000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22982
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..22982
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22982
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12982](-)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12982)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     misc_feature    complement(42..57)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.83"
                     /tool="antismash"
     gene            complement(1087..1542)
                     /gene="tnpA"
                     /locus_tag="HCH27_RS13240"
                     /old_locus_tag="HCH27_13220"
     CDS             complement(1087..1542)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="tnpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /locus_tag="HCH27_RS13240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13220"
                     /product="IS200/IS605 family transposase"
                     /protein_id="WP_065535860.1"
                     /transl_table=11
                     /translation="MSNDDKSLAHTRWNCKYHLVFTPKYRGKVIYGQLRRDIGKILRKL
                     CEMKEVEIIEAHAMPDHIHMLVRIPPKLSVSGFMGFLKGRSAVNIHERHTNLKYNYGNR
                     FFWSKGYYVSTVGLNQKTIAKYIREQEAEAQVKDSINKREYKDPFRK"
     gene            complement(<1716..>2591)
                     /locus_tag="HCH27_RS14335"
                     /pseudo=""
     CDS             complement(<1716..2591)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /locus_tag="HCH27_RS14335"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKDKLNILEMKRKHLFLILAKQPFLLKQTWSMPIIISLKK"
     gene            complement(2635..2799)
                     /locus_tag="HCH27_RS13250"
                     /old_locus_tag="HCH27_13230"
     CDS             complement(2635..2799)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HCH27_RS13250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HCH27_13230"
                     /product="hypothetical protein"
                     /protein_id="WP_231399931.1"
                     /transl_table=11
                     /translation="MTDKLKDKAKEAAGDVTNDKETKTEVDQAVGKVKEITSDVKKGRS
                     SQRCKRKTR"
     gene            complement(<4620..>4969)
                     /locus_tag="HCH27_RS14340"
                     /pseudo=""
     CDS             complement(<4620..4969)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383787.1"
                     /locus_tag="HCH27_RS14340"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSIWPIMNYIRGKSVNWLSLIVLLIFLIYATNQKQMIVIMLVFLV
                     VLAGILYLSTQNILMTLPHILL"
     CDS             complement(5138..5308)
                     /gene="allorf_43013_43183"
                     /locus_tag="allorf_43013_43183"
                     /protein_id="allorf_43013_43183"
                     /tool="antismash"
                     /transl_table=1
                     /translation="METAHLKIHFVKGLSNSFGKNKNKNSTIKFVTASINNGCMWNTVF
                     RNIYRCLTPYK"
     CDS_motif       complement(5249..5308)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: METAHLKIHFVKGLSNSFGK"
                     /locus_tag="allorf_43013_43183_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(5138..5248)
                     /prepeptide="core"
                     /locus_tag="allorf_43013_43183_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-6.90"
                     /molecular_weight="4132.8"
                     /monoisotopic_mass="4130.1"
                     /core_sequence="NKNKNSTIKFVTASINNGCMWNTVFRNIYRCLTPYK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="METAHLKIHFVKGLSNSFGK"
                     /leader_location="[5248:5308](-)"
                     /alternative_weights="4150.8"
                     /alternative_weights="4168.8"
                     /alternative_weights="4186.8"
                     /alternative_weights="4204.8"
     gene            complement(5626..6609)
                     /locus_tag="HCH27_RS14345"
                     /old_locus_tag="HCH27_13235"
     CDS             complement(5626..6609)
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358185.1"
                     /locus_tag="HCH27_RS14345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13235"
                     /product="site-2 protease family protein"
                     /protein_id="WP_260452330.1"
                     /transl_table=11
                     /translation="MKFISPMIFTLLLEIFTLVFELNLVSIAYFALLLSIFVHELGHLA
                     FGLFNKVRPESLIFGFIKLSWENQFKVRLNTQWGFFGGLFRYKPTTFNNKKILRLLTGG
                     PIFSLFFTLTFFIKIDFFQYFSLFNFSIFLITVVPFNFNGFMNDGYNIYKLVTKDYIFE
                     MYYIVSNSLLNKYNQSTFLNTNEVCKIIKKNKELPLYVLNTFLLYVIYEYLIDKNNRKL
                     KLIYPILSKEDKILNNKSYLQNFYLANLYMIEYILSVDNKQTFKKINLKILDSISRSRI
                     KYLNFKCSKSADDEISQSMTEFSNIIKNYPDQTSTMIIAEKQWVQN"
     gene            complement(6610..7848)
                     /locus_tag="HCH27_RS13260"
                     /old_locus_tag="HCH27_13240"
     CDS             complement(6610..7848)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358184.1"
                     /locus_tag="HCH27_RS13260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13240"
                     /product="S8 family serine peptidase"
                     /protein_id="WP_087548481.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.8e-37, bitscore:
                     121.4, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKRGLTYILISYIFFTLGTTGYASDLSNNISFFIDNSQTTAIEE
                     IESELSSEKVDYIQEIGLVSFKNLDDSDRKFIGKYFNVSEGKKLPDFKPEEVNSSILNI
                     NILNKDFKSFNWPYKKILSHIDPVKEQLGKDITIALIDSGIDRLHPNLQDNNLRLKNYV
                     NDIELDEYGHGTQVAGVIDTIAPRVNLNSYKVMDGTDGNSINMLKAIVDATNDQVDIIN
                     VSLGSYKNMEIDDERFTVEAFRKVVNYARKNNILIVASAGNESRDISTGNEKHIPGGLE
                     SVITVGATKKNGDIADYSNYGANVSIYGPAGGYGDNYKITGQIDAREMMMTYYPTSLVS
                     PLGKAADFPDGYTLSFGTSLATPEVSAALAAIMSKNVDNSKDSNEVLNTLFENADSFID
                     KNSMLKYKEVRIK"
     gene            complement(7845..9989)
                     /locus_tag="HCH27_RS13265"
                     /old_locus_tag="HCH27_13245"
     CDS             complement(7845..9989)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415198.1"
                     /locus_tag="HCH27_RS13265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13245"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_221677656.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.9e-43, bitscore:
                     139.2, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRLKYIAQGEHSECALACITMLLNYYGNQSTLVELREKYGVPKG
                     GLTIKNIRTVFDEYGFDVSTFKSSFSNYLDLPTPVISYWNNQHFVVIEKIKKKKVLILD
                     PASNKRWIDISEFKKNFSNILIYAHKKKTKKEGKRKQFFLKSFIFTKFKRYFFSLIILS
                     FVSQLLLLLIPIATKYSIDNIRSFQEIPTYILLLILTSFFSVLYVVQYLKSSVVAEFQY
                     EFDFKLMFSYIDKLFSMPLMYFSNRSTGELVFRANLNIYIRQILSQKVITTLIDSLFLG
                     IYLFLMVNYSILLTIIALVLISLIAFLSIINSHTIKRFVDKEIMEQGNVQRIITEAIEG
                     IETIKSANAEKSFLLNWKNMFTSQLLITKNKNRYIAIFGILPEIIQSVMPALFLIIGIK
                     LIINNSLSLGSLIGFVSIVTMVMKPILSLVSSYNDFLLLNVYFQKLSEVLTYEEKNNFN
                     NKIGNAGKEEFIYRLNNVYYTISVFEKNILNGISFDIRKGDKVAIVGRSGSGKSTLLKL
                     LSGLLQPSNGEILYEGYPLSNNSNNRRNIFYVNQNAHIFNETIEKNISLEFKPNSSINE
                     KKRLKESMNKSKMDEVLLGIPQHEKTIVSENGNNFSGGQRQKIALARAFYSNVNTLLLD
                     EPTSAMDNISEFEVFSNLLDEKRTVITVAHRISTVKNFDKIILMDNGEIVCIGKHEDLI
                     ENSELYRSLYYKKQQFGGTK"
     gene            complement(10001..12982)
                     /locus_tag="HCH27_RS13270"
                     /old_locus_tag="HCH27_13250"
     CDS             complement(10001..12982)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368286.1"
                     /locus_tag="HCH27_RS13270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13250"
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="WP_087548483.1"
                     /sec_met_domain="DUF4135 (E-value: 8.2e-95, bitscore:
                     310.3, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 2.7e-51, bitscore:
                     166.6, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEDNLINVLSINERCFLLKQSGNEKYDIKNLQAWKERKSVLKQDD
                     LDYLIKYKYESLDNFGLGITPIENFPDKEVAIQYIKDQSWYIFFESILDSYNDSEEQLL
                     EVDASYPFRYFLQYARLFLLDLNSELNICTKEFIINLLETLTQELIHLTSKTLVLDLHT
                     FKKNEPLKGNDSSKRFIYYLKKRFNSKKDIIAFYTCYPELMRITVVRMRYFLDNTKQML
                     IRVTEDLPSIQNCFNIQSSELNSISESQGDSHSRGNTVSTLTFSDGKKIVYKPKINSEN
                     KLSDLFEFLNKELEADIYIVKKVTRNTYFYEEYIDNIEINNTEEVKKYYERYGKLIGIA
                     FLFNVTDLHYENIIAHGEYPVIIDNETFFQQNIPIEFGNSATVDAKYKYLDSIMVTGLV
                     PYLAMKDKSDSKDEGVNLSALNFKEQSVPFKILKIKNTFTDEMRFEYQTHIMDTAKNTP
                     IMNNEKISFISYEKYIVTGMKSILMKAKDSKKKILAYINNNLQNLIVRNVIRPTQRYAD
                     MLEFSYHPNCFSNAIEREKVLHNMWAYPYKNKKVVHYEFSDLIDGDIPIFYNNISKTSL
                     IASDGCLVEDFYQESALNRCLNKINDLCDEDISIQTVWLEIALNIYNPYKYINDLKNQN
                     SNKYIYTGSELNSKIIQACQKIEKKIFKRAIFNKKTNTVNWIDIKLDQDWNVGILNNNM
                     YDGLPGIFVFYVALKYITKNHKYDYVIECIKNSIYTIPSEDILSAFFGKGSLIYPLLVD
                     YRLNNDINSLNVAVEIADMLIEKKSINNGELKNDWIHGHNSIIKVLLLLSEITEDEKYR
                     KFSLEIFEKLSEEPYFNFRGFGHGIYSYVHLLSKFNRIDKANSLLHKIKESYFEEEPKN
                     NSWCKGTVGELLATIELYDDNISNIDINKTMAYKNKDCLCHGNAGTLEGLIQLAKKDPE
                     TYQYKKNKLISYMLNDFEKNNTLKVAGSEYLESLGFFVGISGVGYELLRNLDSEIPNAL
                     LFEL"
     gene            complement(13043..13234)
                     /gene="cylL-S"
                     /locus_tag="HCH27_RS13275"
                     /old_locus_tag="HCH27_13255"
     CDS             complement(13043..13234)
                     /codon_start=1
                     /gene="cylL-S"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358181.1"
                     /locus_tag="HCH27_RS13275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13255"
                     /product="lanthipeptide cytolysin subunit CylL-S"
                     /protein_id="WP_002358181.1"
                     /transl_table=11
                     /translation="MLNKENQENYYSNKLELVGPSFEELSLEEMEAIQGSGDVQAETTP
                     ACFTIGLGVGALFSAKFC"
     CDS_motif       complement(13127..13234)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MLNKENQENYYSNKLELVGPSFEELSLEEMEAIQGS"
                     /locus_tag="HCH27_RS13275_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(13043..13126)
                     /prepeptide="core"
                     /locus_tag="HCH27_RS13275_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="2.00"
                     /molecular_weight="2632.0"
                     /monoisotopic_mass="2630.3"
                     /core_sequence="GDVQAETTPACFTIGLGVGALFSAKFC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MLNKENQENYYSNKLELVGPSFEELSLEEMEAIQGS"
                     /leader_location="[13126:13234](-)"
                     /alternative_weights="2650.0"
                     /alternative_weights="2668.0"
     gene            complement(13268..13474)
                     /gene="cylL-L"
                     /locus_tag="HCH27_RS13280"
                     /old_locus_tag="HCH27_13260"
     CDS             complement(13268..13474)
                     /codon_start=1
                     /gene="cylL-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358485.1"
                     /locus_tag="HCH27_RS13280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13260"
                     /product="lanthipeptide cytolysin subunit CylL-L"
                     /protein_id="WP_002358179.1"
                     /transl_table=11
                     /translation="MENLNVVPSFEELSVEEMEAIQGSGDVQAETTPVCAVAATAAASS
                     AACGWVGGGIFTGVTVVVSLKHC"
     CDS_motif       complement(13403..13474)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MENLNVVPSFEELSVEEMEAIQGS"
                     /locus_tag="HCH27_RS13280_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(13268..13402)
                     /prepeptide="core"
                     /locus_tag="HCH27_RS13280_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="2.00"
                     /molecular_weight="4019.5"
                     /monoisotopic_mass="4017.0"
                     /core_sequence="GDVQAETTPVCAVAATAAASSAACGWVGGGIFTGVTVVVSLKH
                     C"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MENLNVVPSFEELSVEEMEAIQGS"
                     /leader_location="[13402:13474](-)"
                     /alternative_weights="4037.6"
                     /alternative_weights="4055.6"
                     /alternative_weights="4073.6"
                     /alternative_weights="4091.6"
                     /alternative_weights="4109.6"
     misc_feature    13877..13892
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 24.29"
                     /tool="antismash"
     gene            13878..14078
                     /gene="cylR2"
                     /locus_tag="HCH27_RS13285"
                     /old_locus_tag="HCH27_13265"
     CDS             13878..14078
                     /codon_start=1
                     /gene="cylR2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370931.1"
                     /locus_tag="HCH27_RS13285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13265"
                     /product="cytolysin regulator CylR2"
                     /protein_id="WP_002358483.1"
                     /transl_table=11
                     /translation="MIINNLKLIREKKKISQSELAALLEVSRQTINGIEKNKYNPSLQL
                     ALKIAYYLNTPLEDIFQWQPE"
     gene            complement(14546..15858)
                     /locus_tag="HCH27_RS13290"
                     /old_locus_tag="HCH27_13270"
                     /pseudo=""
     CDS             complement(14546..15858)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383787.1"
                     /locus_tag="HCH27_RS13290"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="HCH27_13270"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEDRSDIVTNKVEKIDILILSSFVFVVMLIYNFGIEQMEFGDDAF
                     FLKQFTHDFQSNYYEFLKFRYNEWTSRLVIEIALTQAVQHVFLWRILNAIAMSIVAIVP
                     ALLFNADNSRSRLIIGTGMSLLIPASMINNAGWIATTTNYVWVMAAALLSIWPIMNYIR
                     GKSVNWLSFILSLVFLIYATNQEQMVVIMLVSLLVLAGILYLSKMNILITLPHIAIVIA
                     SFVFILTCKGNAARNVQETKQWLPNFSSYSIFDKLQIGYSSTLKALFF"
     gene            complement(15848..15979)
                     /locus_tag="HCH27_RS14350"
     CDS             complement(15848..15979)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159353.1"
                     /locus_tag="HCH27_RS14350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002368288.1"
                     /transl_table=11
                     /translation="MTKEEKILLIVRNSDFTYEELEKLPMSSIDSIYRAHVLEKNGG"
     misc_feature    complement(16314..16323)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 16.05"
                     /tool="antismash"
     misc_feature    complement(16323..16342)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 19.19"
                     /tool="antismash"
     gene            complement(16542..17732)
                     /locus_tag="HCH27_RS13295"
                     /old_locus_tag="HCH27_13275"
     CDS             complement(16542..17732)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012372.5"
                     /locus_tag="HCH27_RS13295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13275"
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_260452331.1"
                     /transl_table=11
                     /translation="MLPLIMILMQLLCMKPITQTQNTIVKVFGSPPERCCQWTASSVVG
                     LAWIAVRWTATVKPRVIILENVEEFKTWGPLIVTKDGPKPDPKKKGKTFQSFVRALERQ
                     DYHVEFKELRACDYGAPTTRKRLFMIARRDHKPIVWPTPTHGSRESKEVAEGKYLPYQS
                     AASCIDWSIPDNSIFGRKKPLRPNTLKRIARGLNKFVLNTVDPYIVRIDQTSYGKVKRT
                     NSIKEPLTTSTNKAEQLLLSPIIGVNTTGHSGTKISAPLATITTSGHHMLISSFLAKNY
                     GSDIGQHLNEPLHTIPTKDRFGLVKVKGSAYRIVDITMRMLQPHELAKAQGVPDTYVLD
                     KGKNGKKISKAKQVARIGNMVVPNCAEALVRANLPELCCNQPFNEQVELLNEDFSW"
     misc_feature    complement(17750..17763)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: weak, score: 15.7"
                     /tool="antismash"
     gene            complement(18583..19097)
                     /locus_tag="HCH27_RS13300"
                     /old_locus_tag="HCH27_13280"
                     /pseudo=""
     CDS             complement(18583..19097)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415202.1"
                     /locus_tag="HCH27_RS13300"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13280"
                     /product="thermonuclease family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSHSLLLSGCTNVEQKAQDFVQDLEQDVYEGFFQTISNDQRIPAD
                     FVRHVDGDTTVLKIEDKEQKVRFLLIDTRRPSNQTQKFSRLA"
     gene            19289..19744
                     /gene="tnpA"
                     /locus_tag="HCH27_RS13305"
                     /old_locus_tag="HCH27_13285"
     CDS             19289..19744
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="tnpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /locus_tag="HCH27_RS13305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13285"
                     /product="IS200/IS605 family transposase"
                     /protein_id="WP_087548485.1"
                     /transl_table=11
                     /translation="MSNDDKSLAHTRWNCKYHLVFTPKYRRKVIYDQLRQDIGKILRKL
                     CEMKEVEIIEAHAMLDHIHMLVRIPPKLSVSGFMGLLKGRSAVIIHERHANLKYNYGNR
                     SFWSKGYYVSTVGLNQKTIAKYIREQEAEDRVRDSINKIEYRDPFSK"
     gene            complement(19931..20083)
                     /locus_tag="HCH27_RS13310"
                     /old_locus_tag="HCH27_13290"
     CDS             complement(19931..20083)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401712.1"
                     /locus_tag="HCH27_RS13310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13290"
                     /product="hypothetical protein"
                     /protein_id="WP_087548486.1"
                     /transl_table=11
                     /translation="MGNENLQKIYADSTKKRTAQQTEWEQAVEGLTFDRLEKKKIEYSL
                     KRKKS"
     gene            complement(20097..20549)
                     /gene="ssb"
                     /locus_tag="HCH27_RS13315"
                     /old_locus_tag="HCH27_13295"
     CDS             complement(20097..20549)
                     /GO_function="GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ssb"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365876.1"
                     /locus_tag="HCH27_RS13315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13295"
                     /product="single-stranded DNA-binding protein"
                     /protein_id="WP_087548487.1"
                     /transl_table=11
                     /translation="MINNVTLVGRLTKDCDLRYTSSGVAVAAFTLAVNRNFTNQNGERE
                     ADYIQCVIWRKPAEALANYTRKGTLIGLVGRLQTRNYENQQGQRIYVTEMVVESFQLLE
                     SKEVSQQRGVNASNPTPVGQQESNTQQPFPDLEGSSIEISDRDLPF"
     gene            complement(20615..>20839)
                     /locus_tag="HCH27_RS13320"
                     /old_locus_tag="HCH27_13300"
                     /pseudo=""
     CDS             complement(20615..>20839)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368294.1"
                     /locus_tag="HCH27_RS13320"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13300"
                     /product="PcfR"
                     /pseudo=""
                     /transl_table=11
                     /translation="HNILVYYAIEQKYMGGITMLYLFYISPYEEDWEMDHQSIVENYQY
                     TYGLNETYPFLSEFGEIKFKNMFGGLVKQ"
     gene            21023..21277
                     /locus_tag="HCH27_RS13325"
                     /old_locus_tag="HCH27_13305"
     CDS             21023..21277
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016614799.1"
                     /locus_tag="HCH27_RS13325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13305"
                     /product="hypothetical protein"
                     /protein_id="WP_087548488.1"
                     /transl_table=11
                     /translation="MNQLEFNELLDKNPQFLKAYLQENLLTKDEAPKYTKQTQAGFDQS
                     AKIGTTIQPFFSKQKNGRTTFKLYLKSEMIEYGKNKRNK"
     gene            complement(21418..21594)
                     /locus_tag="HCH27_RS13330"
                     /old_locus_tag="HCH27_13310"
     CDS             complement(21418..21594)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016626329.1"
                     /locus_tag="HCH27_RS13330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_13310"
                     /product="putative holin-like toxin"
                     /protein_id="WP_087548489.1"
                     /transl_table=11
                     /translation="MKVSTKIYERRSLLSIAEALALMISFGSLIASLIFGILSAVNNDK
                     KSNRSYLWLDNGY"
     gene            21742..21927
                     /locus_tag="HCH27_RS13335"
                     /old_locus_tag="HCH27_13315"
     CDS             21742..21927
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HCH27_RS13335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HCH27_13315"
                     /product="hypothetical protein"
                     /protein_id="WP_231399954.1"
                     /transl_table=11
                     /translation="MDTKTLEHYQKWCQENLLNRTEAMTITKQSKTAFAQSVYTKQLVP
                     FIEKKVTSHPELFVYF"
     misc_feature    complement(21768..21781)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: weak, score: 15.62"
                     /tool="antismash"
     gene            complement(22020..22145)
                     /locus_tag="HCH27_RS14355"
     CDS             complement(22020..22145)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HCH27_RS14355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_255321397.1"
                     /transl_table=11
                     /translation="MKKALQIKLNQEIQISLEQDKSKSTRKEQLIEQIDQVEIGY"
     gene            complement(22278..22889)
                     /locus_tag="HCH27_RS13340"
                     /old_locus_tag="HCH27_13320"
     CDS             complement(22278..22889)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HCH27_RS13340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HCH27_13320"
                     /product="hypothetical protein"
                     /protein_id="WP_231399956.1"
                     /transl_table=11
                     /translation="MTENNSRFDFSDFYEEGFEKTESFEQAIYVFQDGTLWSGYSEDGD
                     GYTRDVDHGSIEAFFKDISIDRYHPDFHSLKLEELIQIVPETRTVLTVMDNHYSQEQVE
                     VLKQLEEENFIIEQLENTLQVGTEKLNSEEYQEGGFEEQNSESYLYDIDIVEENEEIVQ
                     AYNHFLKNIEVDSLSKNNNQYKTKNKINENEQFNGISHDI"
ORIGIN
        1 ccagagtatc attttattac agatggtatt cctcttcatc cagggccaaa tggtcaagtg
       61 atgttaacta aactttttat tgaatcactc ggactaaaac agtatggagg ttatattgaa
      121 tcattagatt atgaattggg atatggaaaa caagaccttg aaatagaagc accggtattt
      181 tttgatatag ataataacgt cgtttcgtta gataaagata aattgagaga attagtaaat
      241 gatgaattaa gtgatgtaac gttaattgct aaggaaaaaa atacaaataa ggagtattct
      301 atttgtgatg ataaaagaat gcttttattg ggaggtttag atgaaaataa agagtatgag
      361 atttatcttt ttgctaaaag tagcaaaaaa ccaattatat ataattggga aaagcatgat
      421 ttgatagttg gggatattac aaggaattta tcaattaagc atactggagg ttatgtgagt
      481 cgatatacaa ttacttggga agaaccatat gaatcaagtt caggagagat tatcaaagaa
      541 aaaaaacaat ggacaggaaa tggtaaggat attttactgg ggaatgtttc aaatattgat
      601 atttcatcaa aagcccggaa cattcaaatc aaaatcgaag aatgtacagg tttggtatct
      661 gagtggtgga aaactatttt tgataaagaa tattcactgt tagatgatga cataactatt
      721 aatattggtg gcacaacatt acacccgaaa gtaatagtaa ataatcattg attctttact
      781 attaaaaaat tgcttacaat agctaataaa agaactatat gtagcaaaaa ttattcaact
      841 gactatttta taatatagag ataaaaggaa cggttaattc ttatttataa aaatagattc
      901 tccattaaat cgtgttggtg aagacaaatt tgaataaaac ttagttgata tgcggtgcgt
      961 tctgtgcgcc cgcatatttt ttgattttta aagattatcc cttgttgtaa ataaatgcgt
     1021 gctctgaaat cgtaaaaatt acgctctaaa aaagccaccc gctacctttc tattcttatc
     1081 tagttgttac ttcctaaatg gatctttata ctctcttttg tttatactat cctttacttg
     1141 agcttctgct tcttgttctc gtatatactt agctatcgtt ttttggttca gtcctacagt
     1201 gcttacataa tagccttttg accaaaagaa tcgatttcca taattgtatt ttaaatttgt
     1261 atgacgttca tgaatgttta ctgcacttct tccttttaag aaacccataa aacctgatac
     1321 actcaacttt ggtggaattc ttaccaacat atggatatga tcgggcattg catgcgcttc
     1381 aatgatttcg acttctttca tctcacacaa ttttcttagt attttcccta tatctcgcct
     1441 caattgacca tatattactt ttcctctata ttttggtgta aacacaagat gatacttaca
     1501 attccaacga gtatgtgcta aacttttatc gtcgttcgac attcaaaatc ccctttcgat
     1561 ttgataacgg ttggtagcct ttatctatta tatcgaaagg agtttttatc gctaaagctc
     1621 tgacttccac gtgcagtacg cgtgtttttt tgtcgaaatc tctttagaga tttcaactga
     1681 ctaaagtcat taatcaaaag cgatttgttt aatcaatatg ggattttaaa ggcattttga
     1741 atgccagaac gataacaaga gaagatcttg gatttttttc taaaagcttg agggaagaca
     1801 ggatatatgt tttttagagt ttcgtgaaag ctttcgtaat tctttggtat agattaggag
     1861 tttgtagatg atcgtatgac tgctttcttt ttttagccga tgatgctgtc gtttgagtga
     1921 ttctagtttg tctgaatttt tgagtattag cttccaatag catttcagtt tcaggtattt
     1981 ttttaatctt cggaactata agcgtgaaag cgatttatga tttgaacacg gaattgatta
     2041 aagctacgac tgatgtgtta cacaatattg gaaccaatct gtgaagattt ctgcgttggg
     2101 aaatcctttt ttcaacagtt ggttatagct ggcattcata tccatcacaa ggtatttcac
     2161 gttcaattgt gtttttctag gaaagcgtat aaagtattgt gcgagtttga ataatcgctg
     2221 atcttcaaaa atcgtgaaca gcatattggt ttatcacatc cacgaaaata aaactcatgg
     2281 ctccagcgat ggatttcatg gatttaaatt tgttcatgca taaacagaag gcaaatattt
     2341 ttaattcgtt tggtaatcct tgggcgcttc gcataagata cttgagattg ttccatccga
     2401 aataaaggac ttttttgcga tcaatttttg tgattggttt tcaaacagat tcatgagtat
     2461 cgcctgtttc atttctttag tgagatgata atgggcattg accaagtttg tttgagtaga
     2521 aaaggttgtt tcgcaagtat gagaaagagg tgtttgcgtt tcatctctag aatattcagc
     2581 ttgtctttga taatttgagc ttgaaaattt ttctacttta tagacactta ttttttatct
     2641 agtttttctt ttacatcttt gactacttct tccttttttt acgtcagagg taatctcttt
     2701 gactttacca acagcttgat caacttcagt ttttgtttct ttatcgtttg taacgtcacc
     2761 agcagcttct tttgctttgt ctttgagttt atctgtcatt taaaccacct tcttaaaaag
     2821 aataatggtc cttgtagggt ctgaacctgc gactggatgg gtatgagccg tctgttctaa
     2881 ccaattgagc taaaagtctt ttattttaat aaaactatat attacttgaa aaaacgaact
     2941 ggtaattata aaatataatt aggaactgaa agtgtcactc ctaaaaaaag ggtaatagtt
     3001 tttatgagct tggggaagtc ttttataaaa aactcttact ctttttattt ttaatcatat
     3061 catactatta taaataatta agctgtataa atgtttgatt ggtagtattt tttaattgat
     3121 ttatacttaa tactttttat tgaaaaaaag tgatgtttgt tatagaatat agtcaccagt
     3181 aggacaataa tggattattc agcttgctta tattttttag tggtgtcctt ttttggagga
     3241 atgttacgct tttattgtat gttagatttt taagtgaaat aatctatgat attaattata
     3301 aaatgtaatc aatttgtgtt taaactttga gagtgtaatc caaatatttt gtagagtaag
     3361 aagattttag aacctatatg tttgtttttc atgatcaaaa gggctaatag aatgggtttt
     3421 ctatcggttc tttgtcaaat aatgttggta aagagaaatt gtagaatcgc gtcaagtcca
     3481 gaggagaaaa tcctctggat tttttatttt gtcttgcttg tcctaaacga aaatcataag
     3541 tatttcgctt tagcactttg ataggattat ttagatactc caactgtcca ctcgaataag
     3601 aataattcaa tgcatgatga gtttattttt ggtatttgtt aaaggttcgt aaggtgattt
     3661 gataagtagc agacaagtga gatactttgt gtaagtaaga cattaaaata cgtttgggtt
     3721 cgtttatgaa tagcatacaa aaagtcttag tataacgagg agttttatgt taactaaata
     3781 ctataggaaa gtagtctgtt cacaagcttt gtttcggtta aataagtttt aaacgaaggg
     3841 cgtcaacgtt ggcttaaact agcttgattt tttgtaatag ctttcaatac tttttaagtt
     3901 gcttactttg ttggttttca gccaaacttg aactttttaa taggcagttt gtccaagtga
     3961 ttcgatggtt tcgaaaggtt cgaccaagat gataaacgat gagcttagcg ttaggtcaac
     4021 atttcttcac tagacaaaca taaggtgcgt acatatcaat ggcaataaat tggacgtttt
     4081 cccacacaga cagtggaaaa ataatgtttt aaatgtggat aaaggctctg acctgacact
     4141 aagaataatt ataaccaact atcaagtctg tataaaggtg aatactttat accgttaaat
     4201 aagtgtggct tctataacac ataaagaatt acttaaaggg aattttttat gttactctga
     4261 gcatagtgac ggtaagtttt ctgaaaagga tgtacagaat gtgaatgtta caatgtccgg
     4321 ttcaaaaatg ctggacagga gcgtatattt tttatccaca ttttctttca attgctatct
     4381 ctgctattaa cgtaaataaa cagtccgttt tactacaaat taaaaatatt aaaatgtatt
     4441 ggctaactaa cctaataaat atctaaaatt ttttgtagtt ttcactacag tatgccgaaa
     4501 tatgacataa tttcactgta gtagacttat ggttttaaag agtaagaggg ggaaatggaa
     4561 aaccggatta ctgatattaa aaaaaattag tgtcaaaata acaacccata ataacaggat
     4621 ggagaccact caaaaaaagc tttcaaagtg aacgaatacc ttatttgaaa cttatcaaaa
     4681 atattgtagt tagaaaaatt aggtagccat tatttcgttt tttgccacat ccccttatat
     4741 attaaaataa acacaaagtt aacgattata ataaaatatg aggtaaagtc atcaggatat
     4801 tttgagtaga cagatataga atacctgcca aaactactaa aaaaactaac ataattacta
     4861 tcatttgttt ttggtttgta gcataaatca agaatatcaa caatacaatt aaggatagcc
     4921 aatttacact ttttcctctt atatagttca taataggcca aatacttaga aatttctgac
     4981 ctgttttttt aggtaaaaat tacacaaact agcttgtgcc ttattcataa catagtattt
     5041 tattgcaaat ccaaagagcc gaatttttag tttcggctct tttttggatc tatttttctt
     5101 taactacttt gggacaagtt tgatccgtaa gggcgaatta tttataggga gttaaacacc
     5161 tataaatatt acgaaagacg gtgttccaca tgcatccatt attaattgaa gcagttacaa
     5221 atttaattgt tgagttctta ttcttgtttt tcccaaaaga gttcgataat cctttaacaa
     5281 aatggatttt caaatgggct gtttccaatt tgttaaagct aattttttac tacgtgaaaa
     5341 gaaaactgga agaacacaga ttaaaatagc caacaacagg aaaaagggta taatttcctc
     5401 ttttctgttg aagtatgccc aacttgaatg tcctgctacg tatctaataa caatttgaac
     5461 atagaaaaga accaacaagg tataccctgt tgattctttt agttaatata ttttaataga
     5521 aaatttattt attacatcat gaaatactgt ataaaaatcg tagcaagcac acatcacttt
     5581 ttttagccta tcattttacg ttgacagtct agctggtttc ttatctcaat tttgaaccca
     5641 ttgtttttct gctataatca tagttgacgt ttgatctgga taatttttaa tgatattgct
     5701 aaattctgtc attgattgac ttatttcatc atcagcactt tttgagcatt taaaatttag
     5761 atatttaatt ctagatctac taattgagtc tagaattttt aaattaattt ttttaaaagt
     5821 ttgtttatta tctacagata agatatattc tatcatatac aaattagcta aataaaaatt
     5881 ctgtaaataa cttttattat tcagtatctt gtcttcttta ctaagtattg ggtatattag
     5941 ttttaatttc ctattatttt tgtctattaa atactcatat ataacataca gtaagaatgt
     6001 atttagcaca tataatggta attctttatt tttttttatt attttgcata cctcattggt
     6061 atttaagaaa gttgactgat tatatttatt aagtaagcta tttgatacaa tataatacat
     6121 ttcgaaaata taatccttag taactaattt atatatattg tatccatcat tcataaaccc
     6181 gttaaaatta aaaggaacta cagtaatcaa gaatattgaa aaattaaata aagaaaaata
     6241 ttgaaaaaag tcaattttta taaaaaaggt taatgtaaaa aaaagactaa atattggtcc
     6301 tccggttaac aacctcagaa ttttcttatt attaaacgta gttggcttat atctaaataa
     6361 tccaccaaaa aatccccact gtgtatttaa tctaacctta aattgatttt cccacgaaag
     6421 ttttataaaa ccaaatatta atgactctgg tctaacttta ttaaataatc caaaagctaa
     6481 atgacctaac tcatgtacaa agatagacaa taacaaggca aagtatgcta tagacactaa
     6541 atttaattca aaaacaagag taaagatttc gagaagtaac gtaaagatca tcggagatat
     6601 aaatttcatc tattttattc tcacctcttt gtatttaagc atgctatttt tatctataaa
     6661 agaatcagca ttttcaaata aagtatttaa tacttcattt gaatctttag aattatctac
     6721 attctttgac ataatagctg ctaaagctgc gcttacttct ggagttgcta aacttgttcc
     6781 aaaagaaagt gtataaccat ctggaaagtc agcagctttg cctagtggag aaactagcga
     6841 tgtagggtaa taagtcatca tcatttcacg agcatcaatt tgtcctgtta ttttgtaatt
     6901 gtcaccatat cctcccgcag gaccatatat agaaacatta gccccataat tagaatagtc
     6961 agcaatatca ccattctttt ttgtagctcc gacggtaatt acagactcta gtcctcctgg
     7021 tatatgtttt tcattaccag tgcttatatc ccgcgactca tttcctgctg atgcaacaat
     7081 tagaatgtta ttttttcttg catagttaac aacttttctg aatgcttcta cagtaaatct
     7141 ttcgtcatct atttccatat ttttatatga gccaagactc acatttatta tatctacttg
     7201 atcatttgta gcatcaacta tagctttaag catatttata gagtttccat ctgtcccatc
     7261 catcaccttg taagaattta aatttactct tggagcaatc gtgtctatta ctccagcaac
     7321 ttgtgtacca tgaccatatt catctaactc aatatcatta acatagtttt ttaatcttag
     7381 gttattgtct tgaagattag gatgaagcct atctatcccc gagtcaatta gagctatggt
     7441 tatatctttc cctagttgtt ctttcactgg atcaatatga gataaaattt ttttatatgg
     7501 ccaattaaaa gacttaaaat ctttgtttaa aatattaata tttaaaatag aactattaac
     7561 ttcttcaggt ttaaaatctg gtaatttttt cccttccgaa acattaaaat atttacctat
     7621 aaacttccta tcactatcat ccaagttttt aaaactaact aatcctattt cctgtatata
     7681 atctactttt tcagaagata attcagactc aatttcttct atagcagttg tttgcgaatt
     7741 atcaataaaa aatgaaatat tatttgagag atctgatgca taacctgttg ttcctaatgt
     7801 aaaaaaaata tatgagatta aaatataagt caatcctctt tttttcattt tgtacctcca
     7861 aattgttgtt ttttgtaata cagtgaacga tataactcac tattttcaat aagatcctca
     7921 tgctttccaa tacatactat ttcaccatta tccattaata taattttatc aaagtttttc
     7981 acggtactaa ttctgtgagc aacggtaata actgttcttt tctcatctaa taaattagaa
     8041 aaaacttcaa attctgaaat attatccatt gcagatgtag gctcatctaa aagtaatgta
     8101 ttcacattag aatagaacgc tctagctaaa gcaatctttt gcctttgacc accagagaaa
     8161 ttattaccat tttctgatac tatagttttc tcatgctgag gaattcctaa aagtacctca
     8221 tccatttttg atttattcat actttctttt agtctttttt tctcatttat agaagaattc
     8281 ggtttaaact ctaaagaaat atttttttcg atagtttcat taaaaatatg cgcattttga
     8341 ttaacataaa aaatgtttct tctattattg gagttgtttg ataaaggata gccttcatac
     8401 aatatttctc cattagaagg ttgtaacaat cccgacaata gttttagtaa agtagatttt
     8461 cctgacccac ttctcccaac aatagctact ttatcccctt ttcttatgtc gaaagaaata
     8521 ccatttaaaa tatttttttc aaaaacagat atagtatagt aaacattatt aagcctataa
     8581 ataaactctt cttttccagc atttcctatt ttattattaa aattattttt ttcttcataa
     8641 gttaaaactt cagataattt ttgaaaatat acatttaata gtagaaagtc attataagag
     8701 gaaaccaaac ttagaattgg cttcataacc attgttacta tagaaacaaa accaattagt
     8761 gatcctaatg acaaagaatt gttgattatt aattttatac ctattattaa aaatagagct
     8821 ggcattacac tttgtatgat ttctggtaat attccaaaaa tagctatata tctattttta
     8881 ttttttgtaa ttaatagttg agacgtaaac atgtttttcc aatttaacaa aaagctcttt
     8941 tctgcgttag cagatttaat tgtttcaatt ccttcaattg cttctgtaat aattctctgg
     9001 acgtttcctt gttccattat ttctttatct acaaatcttt ttattgtatg cgaatttata
     9061 atacttaaaa aagctattaa agagattaga acaagagcta ttattgtcag taaaatagaa
     9121 tagttaacca ttaaaaacaa atatatccct aaaaaaagac tatctattaa agttgttatg
     9181 accttttgag acaatatttg cctaatataa atatttaagt tcgctctaaa cactaattct
     9241 cctgttgatc tattactaaa atacattagt ggcatactaa aaagtttatc aatataagaa
     9301 aacattaatt taaaatcaaa ttcatactga aattctgcta ctacagagct ttttaagtat
     9361 tgaactacat ataaaacaga aaaaaaacta gttaatatta ataacagaat ataggtagga
     9421 atttcttgaa aagatctaat attatctatc gaatactttg ttgcaatagg aattaagagt
     9481 aataaaagtt gtgaaacaaa tgataatatt attagactaa agaaatatct tttgaatttt
     9541 gtaaaaataa atgactttaa aaaaaactgt ttccttttgc cttctttttt agtctttttc
     9601 ttatgtgcgt atattaatat atttgaaaaa ttttttttaa attctgaaat atcaatccag
     9661 cgtttattac ttgcaggatc taagattaat acttttttct tttttatttt ctctatgacc
     9721 acaaaatgtt gattattcca atagcttatt acaggagtcg gaagatctaa ataatttgaa
     9781 aaacttgatt taaatgtcga tacatcaaat ccatattcgt caaagacagt acgaatattt
     9841 ttgatagtta gtcctccttt gggcacccca tatttttccc ttagttctac tagtgtactt
     9901 tgattaccat aataattaag tagcatagtg atgcatgcta aagcacattc actatgctct
     9961 ccttgcgcaa tatacttcaa tcttttcata attacacctc ttataactca aaaagtaatg
    10021 cattcggaat ttcagaatct aggtttctca atagttcata acccacacca ctgattccta
    10081 caaagaatcc tagtgattct aaatactcac ttccagcaac ctttagtgta ttattttttt
    10141 caaaatcatt tagcatatag ctaataagtt tatttttttt atactgataa gtttcaggat
    10201 cttttttagc taattgaatt agcccttcca aagtacctgc atttccatga cacagacaat
    10261 ctttattttt atatgccatg gttttattaa tatctatatt tgaaatgtta tcatcatata
    10321 attcaattgt cgctaaaagt tcacctacag ttcccttaca ccaactattg tttttaggtt
    10381 cttcttcaaa gtatgattct ttaattttat gaagtaaaga atttgcctta tctatcctat
    10441 tgaattttga tagaagatgg acatagctat atataccgtg tccgaatcct ctaaaattaa
    10501 aatagggttc ttcggataat ttttcaaaaa tttctaatga aaattttcta tacttttcat
    10561 cctctgttat ctcggataac aataataaaa cttttattat actattatgc ccgtggatcc
    10621 aatcattttt taactcgcca ttatttatcg atttcttttc aatcaacatg tctgcaattt
    10681 ctacggctac atttaaagag tttatatcat tatttaagcg gtaatctaca agtaaaggat
    10741 atattaaact tccttttcca aaaaaagctg acaatatatc ttcagatggt attgtataaa
    10801 ttgaattttt tatacattca attacatagt catatttatg attctttgta atgtatttaa
    10861 gagccacata aaatacgaat ataccaggca atccatcgta catattatta tttaggatgc
    10921 ccacattcca atcttgatcc aattttatgt caatccaatt tacagtattg gtttttttat
    10981 taaaaatagc tctcttgaaa atttttttct ctatcttctg acatgcttga ataattttac
    11041 tgtttagctc tgagccagta taaatatatt tatttgaatt ttgatttttc aaatcattaa
    11101 tatatttata tggattataa atattcaaag caatttctag ccaaacggtt tgaatagaaa
    11161 tatcttcatc acaaagatca tttattttat ttaggcatct attcaaagcg ctttcttgat
    11221 aaaaatcttc tactaagcac ccgtcactag ctattaatga tgtttttgag atattgttat
    11281 aaaaaatggg tatatctcca tctattaagt ctgaaaattc gtaatgaaca acctttttat
    11341 tcttatatgg ataggcccac atattatgaa gtactttttc tctctctatt gcattagaaa
    11401 agcaattagg atggtatgaa aattccaaca tatcagcata tctttgagtt ggtctgatta
    11461 catttctaac tataagattt tgtaaattat tattaatata tgccaatatt tttttcttag
    11521 aatcctttgc tttcattaaa atacttttca taccagtaac tatatatttt tcatagctga
    11581 taaaactaat tttttcatta ttcataattg gagtattttt tgcagtatcc ataatgtgtg
    11641 tttgatattc aaaacgcatt tcatcagtaa atgtattttt aatttttaat atcttgaacg
    11701 gtacgctttg ctctttaaag tttaacgcac taaggttaac cccttcatct ttcgaatctg
    11761 acttatcttt catcgctaaa tatggtacta gccctgtcac cattatagaa tctaaatatt
    11821 tatatttagc atctactgtt gcgctattac caaactctat aggaatattt tgttgaaaaa
    11881 aagtttcgtt atctatgata acagggtatt ctccatgagc gattatattc tcatagtgta
    11941 agtctgttac attaaataag aaagctattc cgattagttt accatacctt tcataatatt
    12001 tttttacttc ttctgtgtta tttatttcta tattatctat atattcttca tagaaatagg
    12061 tatttctagt aactttttta actatataaa tatctgcctc taactcttta tttaaaaatt
    12121 caaataaatc gcttaattta ttctcgctat taatctttgg cttgtatact atcttctttc
    12181 catcagaaaa agttagtgta gaaacagtat ttccgcgact atgagagtct ccctgtgatt
    12241 ctgatataga gttaagctct gaggattgta tgttaaaaca attttgtatg ctaggtaaat
    12301 cttcagtcac tcttataagc atttgtttag tattatctaa aaaatatctc attctaacaa
    12361 cagtaatacg catcaactca ggatagcatg tataaaaagc tattatatct tttttagagt
    12421 taaatctttt ttttagataa tagataaatc gcttgctact atcatttccc tttagaggtt
    12481 catttttttt aaaagtatgc aaatctagca ctaatgtttt acttgtcaag tgaataagct
    12541 cttgtgttag agtttctaat aaattaataa tgaattcttt tgtacaaata tttaactctg
    12601 agtttaaatc aagtaaaaat aaacgagcat actgtaagaa atatctaaaa ggataactag
    12661 catctacttc taataattgc tcttcactat cattataaga atctaaaata gattcaaaaa
    12721 aaatatacca tgattgatct ttaatgtatt gaattgcaac ttctttatca ggaaagtttt
    12781 caataggtgt tattcctaat ccaaaattat ccagtgattc atatttatat ttaatcaagt
    12841 agtctaaatc atcttgttta agaacacttt ttctttcttt ccaagcttgt aaatttttaa
    12901 tgtcgtattt ctcgttacca ctttgcttta gtaaaaaaca tctttcattt atgcttaata
    12961 catttattaa attatcttcc atgtaagcac tcctttttta tgtataagag ggctagtgaa
    13021 actagccctc ttaatatata aattagcaaa atttagctga aaataatgca cctactccta
    13081 agcctatggt aaaacatgct ggagtagtct cagcctgaac atctccacta ccttgaatcg
    13141 cttccatttc ttctaaactt aactcttcaa aagaaggacc aacaagttct aatttattag
    13201 agtaatagtt ttcttgattt tccttattta gcactgtgct tcacctcact aagttttata
    13261 gtatatttta acaatgtttt aaagacacaa ctacagttac tccagtaaaa ataccgccac
    13321 caacccagcc acaagcagca ctacttgctg cagctgtcgc cgcaacagca cacaccggtg
    13381 ttgtctcagc ctgaacatct ccactacctt gaatcgcttc catttcctca acacttagtt
    13441 cttcaaaact aggaactaca tttaaatttt ccataattct taccctccat cataaaattg
    13501 ttgtatactg cataacataa tatgttacaa tttaatagta tagagctttt aatcttttgt
    13561 caagtggcac ttgacaaaaa ggaggaacca aaatggctat ttttattttt ttattggata
    13621 tcatttctgt agtctacttt gtttttaatc tgactgattc aaaaaaagat gagcgaattt
    13681 tatatacaat ttccttaaca tctacttaca tgtacttatt tattaacttt tccatactta
    13741 tagtaattgc tatttttctt ttactaccta atgttattac taaaaactat ctatttaatt
    13801 ttatctacta ttacttctct ctatcaatga tattgcactc tctgttacta ctttttttca
    13861 acaagaggac ttcataaatg ataatcaata acttaaaatt aattagagag aaaaaaaaaa
    13921 tcagccaaag tgaattagct gctttattag aagttagcag acaaactatt aatggtatag
    13981 aaaaaaataa atataaccct tctttacagt tagcattaaa aattgcttac tacctgaata
    14041 ctccactaga agatattttt caatggcaac ctgaataaaa gagattttta aatttcacca
    14101 cagaatattt aaattaaaag aaaggtgctt cgtatgaaga aaaagtttat atcccttctg
    14161 attttatttt taggaataat tgttggcata gtaacagttt atattttaaa gcataaataa
    14221 ttatgactgt tattttatac tttaatcata actgattttt taacagtaaa accctccctt
    14281 atgattacgt tcatttttat tcttatacag ctcatttaaa ataaaaacta ggctcaaatg
    14341 tataataaag actgtctctt ctggggtgga gacagtctct aaggttataa cttattttag
    14401 cctttcattt ctaacgaata ctcatagaac taaacgattg attcattcag tctgtaagaa
    14461 aatagttaaa tctgaagtga cttgtaatta ttctacagta tacacactat cctttcaaca
    14521 actgtcgaaa gtttagcaaa acattttaca atatttttgt atacatcatt attaataata
    14581 gagccaatac cagaaaatat ccgcatagca ctttaaaggt tctactttgg ttattttcag
    14641 gtagctgact tattaaataa acacctgaca tcattataag aacataagta aaatagtaag
    14701 tccttgatcc agatacccat atggtaggag agaaccccat cataatgcga cttcctacac
    14761 ccaaaataag aagaaaggta actgccaaaa attataaggg attattactt gccccaataa
    14821 tccctattac ataaaaaaaa ccaacccagt aagaagaact actagtgaca tatatgtttt
    14881 tcctgaagct atatcataag tttgattaat gatcgtatta aatcttgtac aaatcaaata
    14941 cgtcaatagc ggtattccac taatcatctt tttgactaat gttccatttt ttactagtcc
    15001 agcagtaaga attagcaata caaaagctaa catcaatgga gaccactaaa aaaataaagc
    15061 tttcaaagtg gacgaatacc ctatttgtaa cttatcaaaa atactgtagc tagaaaaatt
    15121 aggtaaccat tgtttcgttt cttggacgtt tcttgccgca ttccccttac atgttaaaat
    15181 aaacacaaag ctagcgatta caatagcaat atgaggtaaa gttatcaaga tattcatttt
    15241 agataaatat agaatacctg ccaaaactaa taaagaaact aacataatga ctaccatttg
    15301 ttcttgattt gtagcataaa tcaagaacac caacgataaa ataaaggata gccaatttac
    15361 actttttcct cttatatagt tcataatagg ccaaatactt aacaacgctg ctgccatgac
    15421 ccaaacataa tttgttgttg ttgctatcca tcctgcatta tttatcatcg aagcaggaat
    15481 taataatgac atgccagtac ctataattaa tctacttcga ctattatctg cgttaaacag
    15541 tagtgcaggg acaattgcca ctatagacat tgctatagca tttaaaatcc tccacaaaaa
    15601 tacgtgttgt accgcttgtg ttagagctat ttcaatcact aatcgggacg tccactcatt
    15661 gtatctaaac ttcaaaaatt cataatagtt actttgaaaa tcatgtgtaa actgctttaa
    15721 gaaaaacgca tcgtccccaa attccatttg ttcaatacca aaattgtaaa ttaacattac
    15781 cacaaaaaca aaactactga gaattaaaat atctatcttt tccactttat ttgtcactat
    15841 gtcactccta tcctccattt ttttcaagaa catgcgctct ataaatacta tcgatacttg
    15901 acattggaag cttttctagt tcttcatacg taaagtcact attacgtaca atcaatagta
    15961 tcttctcttc tttagtcata tatcttccct cactttcatt gttttatcta tttttttacc
    16021 ctctttataa ttgaacacaa tgtttagctg cactttgtat tcataaacat taaggcatta
    16081 atccattctt acgaaaaaat attccaattt ataaaggact ttatttaatt tgattttttt
    16141 cgtttctaat cagtttctca tagtactttt gcgctttttg attggtaaaa gcagtttatg
    16201 tctcccattt ttgggattat cttaacttcc attttcacct atctgaaagt tttcgtcaat
    16261 gtcaaagtta acgcttgacg tatagtcaag taaatgttcg cgattcacaa tcggttcccc
    16321 ttcaagggat ttaactcccc ataaatcgct ttgcttttct cacacggtaa tatctaaact
    16381 agattgtccg ttattaacat tcccaacgga ctttgaacta ctttttaagt caaggttaac
    16441 tacctaatta tattattcct aaccatatcc gggaacattc ttgttctttg atccatttgt
    16501 aaattagtat tcgactgctt cttttctgta ctagtcagtt gctaccagct aaaatcctca
    16561 tttaacaatt ctacttgctc attgaacggt tgattacaac ataattcagg taaatttgct
    16621 cttactaaag cctcagcgca atttggcaca accatgtttc ctattctagc cacctgttta
    16681 gccttactta ttttctttcc gttcttacct ttatctaata catatgtgtc tgggacacct
    16741 tgtgcttttg ctaattcatg aggttgaagc attcgcattg tgatatcaac gattctatag
    16801 gcacttcctt taacttttac taacccaaat cggtcttttg tagggatcgt atggagtggt
    16861 tcattcaaat gttgccctat gtcagatcca tagtttttcg caagaaaaga cgaaatcaac
    16921 atatggtgac cactagtagt gatagttgct aatggagcag atattttcgt tccagagtgt
    16981 cctgtagtat tcacacctat aattggactg agtagcagct gttctgcttt atttgtgctc
    17041 gtcgttaaag gctcctttat cgaatttgta cgtttaactt tcccataact agtttgatca
    17101 attctaacaa tatacggatc taccgtatta agtacaaact tgtttagtcc gcgagcaatt
    17161 cttttcaacg tattaggacg taaaggtttc ttacgaccaa aaatagaatt atctggtatt
    17221 gaccaatcaa tacaacttgc agctgattga taaggaaggt atttcccttc agcaacttct
    17281 ttactttctc ttgacccatg cgttggtgtt ggccaaacaa tcggtttatg atcccggcgt
    17341 gctatcataa ataaccgttt tcgtgtagta ggcgcaccat agtcacacgc acgtaactct
    17401 ttaaactcaa catgatagtc ttgacgttcc aatgctctca caaaggattg gaatgttttc
    17461 ccctttttct tgggatctgg ctttggacca tctttagtga caatcaaagg accccatgtc
    17521 ttaaattctt cgacattctc caaaataatg acacgtggtt taacagtagc tgtccaacga
    17581 acagcaatcc atgctaaccc tacaacgcta ctggccgtcc attggcaaca tctctcgggt
    17641 ggactcccaa acactttcac aatagtgttt tgtgtttggg tgattggctt catgcatagc
    17701 aattgcatca ggatcatgat taatggcaac atctaccgaa cgtcctattg catgctctgt
    17761 gcctgtgcta gcatcaccac cgcccgcaaa attatctaca acaatttcct tcaaaaaatc
    17821 accttcctaa cttaacctac atttttccat cctaaaatat gtcactttta agctattctc
    17881 caaatacctt tagtaactca tagcgacctc taatcaattt ttccattgtg tgcgctggat
    17941 aatatctttt cttattgtct gactagcttt tttcataaca gactatttct tcccacattg
    18001 tggcggcttc ttcagatact tcaaaataaa tcctcttctt ttgctgtgta ccttacttat
    18061 ttaggtgtgt acctgtcatg tcctcatctc tcttagaaac cgttatttta aattattttt
    18121 gtataaccat catgttactc cagtttccta cttttatttt caatttttcc atcgcatttt
    18181 cagctctctt ttttgacgtg tagtctttcg catttcttat atcgtccaca aaattgccaa
    18241 atacttctcc ttgaacagta tatgttgtgc ctgaatagta atactattgt tcaacaccac
    18301 caccaaaatt ttgagaaact ttaattacaa aattcttttt acatcccatt ttcctgtctc
    18361 ccttttcaaa tttttttaga actaactctt atttttcttt agtcttttct cttacttctc
    18421 cgcgatactt tctcccttgt ggtgttttaa gaaaatcaac tagtttctcg tttgattcaa
    18481 actttttaat ttttcttgtc attttattgc tacatattca aattcagtca taatcattct
    18541 cccttattta aaccctttgt ctgtcacata gcctggtata ctctagattc tgagtgattc
    18601 actttttgcc tgttcttggg cttgctctag ttctgacaaa tacttagtgt taggttcttt
    18661 gacataagct acacgtgcta atccctctct cacaagtgtt ttttgtagta atgttccatc
    18721 tacaaaaata taacccaacg ctctaccgta acgatctttt ttattccctt gatcatattc
    18781 aaaagtaatt tctgaagtag tagacaaaag ctcttttgta cgtttgctag cttctaagcc
    18841 aaacggctga acttttgtgt ttggtttgac ggtctccgtg tatcgattaa taaaaagcgt
    18901 actttctgtt ctttgtcttc aatcttcaaa accgttgtgt caccgtctac gtgacgaaca
    18961 aaatcagctg gtatacgttg atcatttgaa atagtttgaa aaaagccttc ataaacatct
    19021 tgttctaggt cttgaacaaa atcttgtgct ttttgttcaa catttgtaca acctgataat
    19081 agaagagaat gggacaataa tcagaaaaaa atctgattat tgtctcattt ttttgattaa
    19141 tgactttagt cagtcgaaat ctctaaagag atttcgacaa aaaaaccacg cgtactgcgc
    19201 gtggaggaac tagctttagc gataaaaact cctttcgata taatagataa aggctaccaa
    19261 ccgttatcta atcgaaagga gtttttgtat gtcgaacgac gataaaagtt tagcacatac
    19321 tcgttggaat tgtaagtacc atcttgtatt tacaccaaaa tatagaagaa aagtaatcta
    19381 tgatcagtta aggcaggata ttggaaaaat actgagaaaa ttgtgtgaaa tgaaagaagt
    19441 cgaaatcatt gaagctcatg caatgcttga ccatatccat atgttggtaa gaatcccgcc
    19501 gaagttgagt gtatcaggat tcatgggttt gttaaaagga agaagtgcag taatcattca
    19561 tgaacgtcat gcgaatttaa aatataatta tgggaatcgg tcattttggt caaaaggcta
    19621 ttatgtgagt actgtaggtc tgaaccaaaa aacgatagct aagtatatac gagaacaaga
    19681 agcagaagat cgagtaagag atagtataaa caaaatagag tatagagatc catttagtaa
    19741 gtaacaacta gttaatgata gaaaggtagc ggttggcgat ctattaaaaa agctccctta
    19801 aagggagcgg caggtagcga gcccttctag ggctattaaa aagccacccg ctatgcgggt
    19861 ggatatttac ttggagaaag gactattgtc ccacgctctt ttccaataaa tagtttcatt
    19921 ttctaactcc ttaacttttt ttccgtttta aagaatactc aattttcttt ttttctaacc
    19981 tatcaaacgt taatccttca acagcttgtt cccattctgt ttgttgtgct gtacgtttct
    20041 ttgtactgtc tgcatatatt ttttgtaaat tttcattacc catgaatcaa tcctccttaa
    20101 aacggtaagt cacgatctga aatctcaata gaactacctt ctaaatctgg aaagggttgt
    20161 tgcgtattgc tttcctgctg tccaactggt gtaggattag aagcgttaac accacgttgt
    20221 tgactaactt cttttgactc taacaattgg aagctttcta caaccatttc tgtcacataa
    20281 atacgttgtc cttgttgatt ctcatagtta cgtgtttgaa gtctacccac aagaccaatc
    20341 aatgttcctt ttcgtgtata gtttgctaaa gcttctgcag gtttacgcca aatcacacac
    20401 tgaatatagt ctgcttcacg ttcaccattt tgatttgtaa aatttcggtt aactgctagt
    20461 gtaaaagctg caacagctac accacttgac gtataacgca aatcacagtc ttttgtaagt
    20521 cttcctacta aagtcacatt attaatcatt taaaaaacct ctttctatta tttttttaga
    20581 agtgatagca actagtacta tcacttctaa atatttactg ttttactaaa ccaccaaaca
    20641 tatttttaaa cttgatttca ccaaactctg aaaggaacgg atacgtttcg tttaagccgt
    20701 aagtgtattg gtaattctct acaatacttt gatggtccat ttcccagtcc tcttcatacg
    20761 gagatatata aaacaagtac aacatcgtta taccccccat atacttttgt tcaatcgcat
    20821 agtatacaag tatattgtgc aatcgtcgct tcatgtattc ccattaatcg cattcttttt
    20881 agtgcttctt ctttttttca ttattcattt tgttccctcc atctcttaac cttatatcta
    20941 tatcatactt tataaagtat tgtattgtat ttttttactt tataaggtat actttatata
    21001 tcaataaaga ggagggataa cattgaatca actagagttc aatgagctat tagacaaaaa
    21061 tcctcaattt ttaaaggctt atctacaaga aaatttgtta actaaagacg aagcacctaa
    21121 atatacgaag caaacacagg ccggctttga tcaatctgca aaaatcggaa caactatcca
    21181 accatttttt tctaaacaaa aaaacggccg caccacattt aagttatatc taaagtctga
    21241 aatgatagaa tatggaaaaa acaaaagaaa taaataaaaa gaccacctaa aaatcaggaa
    21301 gtctttcact agtatttcag caccccttgt gctaatatat aaatgaaaag agagctgtgc
    21361 gctaacacaa ctctctggtg tagaaccgtt taagacggta gcagatttaa ttacagatta
    21421 gtaaccgtta tctagccaaa gatatgaacg gttacttttt ttgtcgttgt ttacagcgct
    21481 caagattcca aagattaagc ttgcgattaa agaaccgaaa ctaatcatca gagctaaggc
    21541 ttctgcgata gacaaaaggc ttctcctttc atagatttta gtacttactt tcataagcac
    21601 caccacctct cggaagatgc caccgccata acttttctac ttacttagta taccacaaaa
    21661 cagatagttc ttccttacat acttattgcg cgagacatgt tctttaagct atgataaaaa
    21721 aacaatgaaa gggctgataa tttggatact aaaacacttg aacactatca aaaatggtgc
    21781 caagaaaact tactcaatcg aacagaagca atgaccatca cgaaacaaag caaaacagcg
    21841 tttgcacaat ctgtttatac aaaacagtta gtaccgttta ttgaaaaaaa ggtgacaagc
    21901 catccggaac tgttcgttta tttttaaaac aagacgtaga agcatacgca ataagaatac
    21961 agaacagaca acagaatcaa ccaacaaaaa agccatcaca ataactggga tggctttttt
    22021 tagtaaccaa tctctacctg gtctatttgt tctatcaatt gctcttttct tgtagatttt
    22081 gatttatctt gttctaaact tatttgtatt tcttgattca atttgatttg taatgctttt
    22141 ttcattcaac tctttttcct gtgggaaagt tcctgcttgt cgcttcaggg gtcaacttta
    22201 tcttcctttc attccccctc acgccaatca tatctaatta gatatgattg gccaagtaag
    22261 gcgataacgt attttattta aatatcatga ctaattccgt taaattgttc attttcattt
    22321 attttatttt tcgttttgta ctgattattg ttttttgaca aggagtctac ttctatattt
    22381 tttaaaaagt gattatatgc ttgaactatt tcttcgtttt cttcgacaat atcaatatca
    22441 taaagataac tttcagagtt ttgttcctca aaaccgcctt cttgatattc ttctgaattg
    22501 agcttttcag ttcccacttg tagagtattt tccaactgtt caataataaa attctcctcc
    22561 tctaactgct tcaatacctc aacttgttct tgtgaataat gattatccat cactgttaat
    22621 actgtacgtg tttcaggaac aatctgaatt aattcttcta actttaatga atgaaagtca
    22681 gggtgatacc gatcaataga aatatcttta aaaaaagctt caatagatcc atgatcaaca
    22741 tctctcgtgt aaccatctcc gtcttctgaa tagccactcc ataaagtccc atcttgaaaa
    22801 acataaatgg cttgttcaaa gctttctgtc ttttcgaaac cttcttcata aaaatctgag
    22861 aagtcaaacc tagaattatt ttctgtcatt ttccatcctc tttctttttt gaataaaaaa
    22921 agagaataac tattagttat cctcttacaa ttcctattta ttttttagat attcatactt
    22981 tg
//
