LOCUS       NZ_JAASJP010000001     20729 bp    DNA     linear   CON 27-SEP-2024
DEFINITION  Enterococcus faecalis strain K58 scaf_1, whole genome shotgun
            sequence.
ACCESSION   NZ_JAASJP010000001
VERSION     NZ_JAASJP010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600540)
  AUTHORS   Lv,Q. and Li,S.
  TITLE     Detailed information of the 97 whole-genome sequenced gut bacterial
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 600540)
  AUTHORS   Lv,Q. and Li,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2020) College of Animal Science and Technology,
            Heilongjiang Bayi Agricultural University, Liming Street, Daqing
            163319, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019731285.1-RS_2024_09_27
            Annotation Date                   :: 09/27/2024 07:58:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,794
            Genes (coding)                    :: 2,742
            CDSs (with protein)               :: 2,742
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 22 of 52
            Pseudo Genes (incomplete)         :: 35 of 52
            Pseudo Genes (internal stop)      :: 13 of 52
            Pseudo Genes (multiple problems)  :: 14 of 52
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:25:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 253989
            Orig. end                         :: 274718
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAASJP010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20729
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20729
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20729
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10729](+)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10729
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     misc_feature    11..25
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.5"
                     /tool="antismash"
     gene            172..567
                     /locus_tag="HCH27_RS01180"
                     /old_locus_tag="HCH27_01180"
     CDS             172..567
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407764.1"
                     /locus_tag="HCH27_RS01180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_01180"
                     /product="hypothetical protein"
                     /protein_id="WP_002357220.1"
                     /transl_table=11
                     /translation="MDGMFANLTLRERRLLKQAQVGATISFILLFVPILTISLLHTSDL
                     LTKWLGIVGVIMVLPLLYFAWQILKIAYKDQKDNPTPPLWVPKVYGIGLSINPYHRFGK
                     LIFILLAVLIVGIIISLLFTPASQINH"
     gene            1071..6698
                     /locus_tag="HCH27_RS01185"
                     /old_locus_tag="HCH27_01185"
     CDS             1071..6698
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633936.1"
                     /locus_tag="HCH27_RS01185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_01185"
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="WP_185774490.1"
                     /transl_table=11
                     /translation="MGSNFKEEKMNKEYQGKKIYGKKHWLTFLLVSSSVLAFHSFVTMD
                     SQVVDAQEQTAKEDVADSATSVGAIVSIEKAEKNFVITYASGKKAQISILNDHLFRYHL
                     DPTGKFEEYPTPNDPKHVAKITAKTMADYGTQAFEQTNVTDSGNQFILENNGLKIMFEK
                     ESALMKVLDKKKNQVILEETAPLSFKNDKATQTLKQSSQENYFGGGTQNGRFTHKGTAI
                     QIVNTNNWVDGGVASPNPFYWSTAGYGVVRNTWKPGNYDFGSHDPQTTTTTHEGTDFDA
                     FYFFNDSSAGILKDYYELTGKPALMPEYGFYEAHLNAYNRDYWVKVAEGTAGAVKFEDG
                     NFYKEYQPGDLGNLNGTLESLNGEKENYQFSARAVIDRYKKNDMPLGWFLPNDGYGAGY
                     GQTDSLDGDVQNLKEFTEYAQANGVEVGLWTQSNLHPADPKNPKKGERDIAKEVSVAGV
                     KALKTDVAWVGYGYSFGLNGVEDAANVFVKETDGAVRPMIVSLDGWAGTQRHAGIWTGD
                     QTGGQWEYIRFHIPTYIGTSLSGQPNVGSDMDGIFGGKNKEVNIRDFQWKTFTPVQLNM
                     DGWGSNPKTPFAFDQEATDLNRAYLKLKSMMMPYNYSIAKESVDGLPMVRAMALEFPNE
                     GTAYTKDSQYQYMWGPNLLVAPIYNGNQDEAGNSIRDGIYLPDEKQVWVDLFTGEKYQG
                     GRVLNGVKTPLWKVPVFVKDGSIIPMTNPNNNPKEIQRDQRSFLIYPNGTTSFNMYEDD
                     GISTSYEAGQSATTKINSQGPKSNEKGDLTVTIEPTKGSYKGFVDERSTTLDLLASEAP
                     ESVTAMVGGTEVTLKQAANKEEFLAGTNLYYFDKEFQVNQYLSEASGEKLNQSALSVKL
                     AKQSVTAKDVQITVKGFINKGTVDGGNTTVDDQLTIPANVAINEEKTTPSSLTLQWDQV
                     TEATSYEVERDGTVFGNIQTNTATFDGFSFLSEHTFRVRAVGKNGVSEWSEPIKGKTQD
                     DPYKETINQVKATSNLPEQPGAELKKLTDKDLSTGWHTNWSTGIANPSDGNFLSLKFDL
                     GAEYQMDKIEYLPRDNAGNGNILQLQYRTSKDGANWTEFSEPINWKQDALTKTIETKDQ
                     AYRFVEMKVLKSVGNFGSGREMLFYKQPGTEGILHGDITNDGTIDENDAMSYRNYTGLE
                     SVDSDFNGYVEKGDLNKNGVIDAYDISYVLRQLDGGIEIPDVEEIAGGLSLAVVNENGK
                     DTYLPGDTLTFTLKGQDLKNINALSTKMSFDSSKFELVGQPATTNNTKQMENYSKYRKH
                     SNGVENLYLVLSNQGNKQLLNGSMDLVTFKVKVKETTRVKHATTVEQPLQFDMSQGLLV
                     GQGFQQATLSDFSVTVKPTELVDKELLQALITLNQARVEKEYTPETWAIFKPVLDEAVA
                     VLANEQATQTDVSAAVENLEKAASQLEKMPDVANKADLEKAIQEGLAKKPSDGQEFTEE
                     TKKVLEDSLAAAQKVFAQEKVTQEEIDQATKTLREAIAQLKEQPVAVDKETLKEKIAQA
                     RGRKPEEGYQFTKETEKQLQEAIQAAEAIVAKETATKEEVSEALNALETAMAQLKEVPL
                     VNKDQLQEVVKRAQQVTPSEGHQFTASSLQDLQKALLAAKNTLENPAANQKMIDEAVAE
                     LTSAIDGLQEEALVTDKKALEAMIAKAKAIKPSAGKEFTSESKARLTEAIDQAEGILAD
                     KNARQEQIDTAEKNVKTALDSLEEQVLQTDKTKLKELLQKAETLKPKAGKQFTKASQEA
                     LAEAIKQAKALVEDPNATQEAVDKCLSILAQAIEAMAEEPISSNSTGNNGNHGTVSGTG
                     GVTSPGKGTAAGGTTTKTTTSGTLPKANEVVSPIWSISGFLLIVSIGLGKLFFKNKKDQ
                     N"
     gene            6780..8762
                     /locus_tag="HCH27_RS01190"
                     /old_locus_tag="HCH27_01190"
     CDS             6780..8762
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401391.1"
                     /locus_tag="HCH27_RS01190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_01190"
                     /product="amidase domain-containing protein"
                     /protein_id="WP_002425767.1"
                     /transl_table=11
                     /translation="MKRKKIKNQLLVLTSILVLLSLEVAPVVTLAAELPSSSLQTALSS
                     ETTITSEEKVIETTETTETTVATSTTSTSSSSSESNSSTDTTTESTSHSTAETTTTNTS
                     SETKKEPTESTVSSEITQPVEQSQPTQVQPPQVPVTKQEPEEPIQVPEATNNVVEENQA
                     VSLNPSLKVDEIASSNLKGYELPLLSSFGEKKRAVVVAEALRHVGKTKKEFNLTEQALT
                     SSFLAQKIYQQLFKIDIGSTPQEQITFGKVRSIEEAEPGDLIFWQTAEGKTLQNGVYLG
                     QGKYLIAAAEADSKEEPEVIGQLENIYTAKQQPDSKEEKRLVVTNPFKEFTLTEYGKEV
                     LATYGASFEMQKSEQTTAFIKKIGETARELGEKYDVFASVMIAQAILESGSGESQLAKE
                     PYYNLFGVRGSFQGNSVSFSTKEADQRGQLYTISAGFRDYGGYNDSLQDYVQLLRQGID
                     GNQDFYKPAWRSEAKNYLQATRFLTGKYATDKQYDNKLNSLIAVYNLTQFDLPKTVDGL
                     IIQSKNKLSEAEQQQMHFPVYDGINYNRSGSYPVGQCTWYVYNRFKQLGTSVDEFMGNG
                     SDWGRKGRALGYQVSSLPKAGRAISFQPGVAGADNQYGHVAFVEAVTSDGIIISESNVI
                     NDQTISYRVLPNVIAYSSGVTYIGA"
     misc_feature    complement(9054..9069)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.55"
                     /tool="antismash"
     gene            9181..9912
                     /gene="fsrA"
                     /locus_tag="HCH27_RS01195"
                     /old_locus_tag="HCH27_01195"
     CDS             9181..9912
                     /codon_start=1
                     /gene="fsrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109506.1"
                     /locus_tag="HCH27_RS01195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_01195"
                     /product="response regulator FsrA"
                     /protein_id="WP_002414298.1"
                     /transl_table=11
                     /translation="MAIYILEDQIIQAKALEALVTNILHSRNIYNETIHLFSRSDELLQ
                     VAHQDAQLNIFFLDIQMNNHIQAGFEVAKEIRKTDSESLIVFISTHTELVLTSYKYMVS
                     ALQFIQKNVDFLDFRKEVETCVDAYIQQKENIKTKSEYIIINLKASSIKMDINDIYFFQ
                     TEYDHRVSMVGKNFKREFYGTLSKIEQLHPDLIRVHQSIIINKKYASKLNYKTHLLTMR
                     DGTEVPVSRRYYTQVKALFLT"
     gene            10001..10729
                     /locus_tag="HCH27_RS01200"
                     /old_locus_tag="HCH27_01200"
     CDS             10001..10729
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634271.1"
                     /locus_tag="HCH27_RS01200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_01200"
                     /product="accessory gene regulator AgrB"
                     /protein_id="WP_002377181.1"
                     /sec_met_domain="AgrB (E-value: 2.4e-32, bitscore: 106.9,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLIDWILKNIMDMDQEDQSGKTQWTKYYLTVYFSGLFNFLMILIL
                     SVLFGTLSETFIVYVVLIFLRPVAGGWHAKTKWLCRLESIVIYVAIPFVLKNSSVSLPF
                     IYKILLICLLVVLFYWYAPQGTAIEPVQPSDLNVLKKQSLIRVCLLILCSLFVKEKIAS
                     VILYGLVIQGLMILPVTKNLIEGSVFMKFGKKIIKNVIEKRVAKVSDGVGTKPRLNQNS
                     PNIFGQWMGQTEKPKKNIEK"
     gene            10726..12069
                     /gene="fsrC"
                     /locus_tag="HCH27_RS01205"
                     /old_locus_tag="HCH27_01205"
     CDS             10726..12069
                     /codon_start=1
                     /gene="fsrC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402207.1"
                     /locus_tag="HCH27_RS01205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_01205"
                     /product="sensor histidine kinase FsrC"
                     /protein_id="WP_010820044.1"
                     /transl_table=11
                     /translation="MILLLLATNVLLVSSFIVFVFLRVTLIKIECKIPLLSLLIVINLC
                     SFAALMLGYSWLIYALTVVIFTGFLLIHKKRFSIFKAIFLSVFTLLMVSFINYTEQTIL
                     SVFFQQIYQNKLLWIASNVLLLLINIWIALKIPNSVFLRLNRVLENSRIFFGCLLLLLI
                     LLLLFVFLISPEISPDFMRGFVTVNSSKLELLISVGLFLILIGLVIEAYLEEQRINTQL
                     LNNLTIYTEKIESINEELAMFRHDYKNLLYSLQIAISYEDILEIKRIYEETIAPTKKII
                     DNEEFELMKLNRLKNMELKALISMKINTAKQAKLKVIVDVPEVFILDTSIDLVVVIRLL
                     AILLDNAIENSAKSELKMFAISIFNKNETQEFVITNSVQAEFDFRVMKKTKFSSKSNPE
                     EHGWGLLYVKEIVDFSDQFDLQTSFNEGAVTQHLIIEKNHNSKKVVNE"
     gene            <12289..13578
                     /gene="gelE"
                     /locus_tag="HCH27_RS01210"
                     /old_locus_tag="HCH27_01210"
                     /pseudo=""
     CDS             <12289..13578
                     /codon_start=1
                     /gene="gelE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369251.1"
                     /locus_tag="HCH27_RS01210"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_01210"
                     /product="M4 family metallopeptidase coccolysin"
                     /pseudo=""
                     /transl_table=11
                     /translation="EGVTVDSDNVIIHLDKNGAVTSVTGNPVDQVVKIQSVDAIGEEGV
                     KKIIASDNPETKDIVFLAIDKRVNNEGQLFYKVRVTSSPTGDPVSLVYKVNATDGTIME
                     KQDLTEHVGSEVTLKNSFQVTFNVPVEKSNTGIALHGTDNTGVYHAVVDGKNNYSIIQA
                     PSLATLNQNAVDAYTHGKFVKTYYEDHFQRHSIDDRGMPILSVVDEQHPDAYDNAFWDG
                     KAMRYGETSTPTGKTYASSLDVVGHEMTHGVTEHTAGLEYLGQSGALNESYSDLMGYII
                     SGASNPEIGADTQSVDRKTGIRNLQTPSKHGQPETMAQYDDRARYKGTPYYDQGGVHYN
                     SGIINRIGYTIIQNLGIEKAQTIFYSSLVNYLTPKAQFSDARDAMLAAAKVQYGDEAAS
                     VVSAAFNSAGIGAKEDIQVNQPSESVLVNE"
     gene            13627..14481
                     /gene="sprE"
                     /locus_tag="HCH27_RS01215"
                     /old_locus_tag="HCH27_01215"
     CDS             13627..14481
                     /codon_start=1
                     /gene="sprE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364098.1"
                     /locus_tag="HCH27_RS01215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_01215"
                     /product="serine proteinase SprE"
                     /protein_id="WP_010820043.1"
                     /transl_table=11
                     /translation="MKKFSIRKISAGFLFLILVTLIAGFSLSANAEEYIVPAESHSRQK
                     RSLLDPEDRRQEVADTTEAPFASIGRIISPASKPGYISLGTGFVVGTNTIVTNNHVAES
                     FKNAKVLNPNAKDDAWFYPGRDGSATPFGKFKVIDVAFSPNADIAVVTVGKQNDRPDGP
                     ELGEILTPFVLKKFESSDTHVTISGYPGEKNHTQWSHENDLFTSNFTDLENPLLFYDID
                     TTGGQSGSPIYNARFEVVGVHSNGGIKQTGNHGQRLNEVNYNFIVNRVNEEENKRLSAV
                     PVA"
     gene            complement(14629..15459)
                     /locus_tag="HCH27_RS01220"
                     /old_locus_tag="HCH27_01220"
     CDS             complement(14629..15459)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397393.1"
                     /locus_tag="HCH27_RS01220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_01220"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_002371935.1"
                     /transl_table=11
                     /translation="MKINYLGTGAAERIPGIFCNCKLCKYAREKKGREIRTQTQLIIDD
                     ILLIDFPGDSYLHLLQYDLCFADFNSLLISHWHSDHFYGEDLAYRMSGYALDIPNRLTV
                     YGNEAVKTFYQRAFDLEGRQDDTRLHYQVIQPYKCFTIANYTIYPLPAQHGNFSEDCFI
                     FVIDDGKDAFLSTHDTGYFTSEMFEYLEKSHLLLSIVSLDCTSQTNETGNSHMNWEENL
                     KLIAELKERKLVTDKTIYVANHFSHNGGLSYAEMAALSQKHEIITSYDGLEILT"
     gene            15851..16705
                     /locus_tag="HCH27_RS01225"
                     /old_locus_tag="HCH27_01225"
     CDS             15851..16705
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821679.1"
                     /locus_tag="HCH27_RS01225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_01225"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_002357233.1"
                     /transl_table=11
                     /translation="MTIEKLEYFYTIAKYNSISKAASELHVSKSTLSASLKDLESELGH
                     LLFDRNGNSLTLNSYGDKIVQSVYIILNEAKKMKLNLHEMIENPVMRLGFGNTSLMYKV
                     TENEDQLNRFWECYHGSSFELLNKLENHELDFVITSADVNSPVLKKEQLIELKMYLCVS
                     REIKQEIEEEGFTCLTNYPFLFLPHHLDHLEATKSVLEMLQLTSPLVCCYDTLMLTRLI
                     EKSKGVYAVISLRKEQLQEIDSKLFFLPIERKQKFYLYRNVSSSVFVQPGQIKATLQKL
                     LET"
     gene            17087..18475
                     /locus_tag="HCH27_RS01230"
                     /old_locus_tag="HCH27_01230"
     CDS             17087..18475
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010712556.1"
                     /locus_tag="HCH27_RS01230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_01230"
                     /product="MFS transporter"
                     /protein_id="WP_010712556.1"
                     /transl_table=11
                     /translation="MIDRKKVILYTCCMSLFVVTMDVTVVNVALPSIQSDFHTNLSTLQ
                     WVTDGYTLMVASLLLLSGSTADRIGRKRVLQLGLACFGLASFLCGISQTPGQLIAFRML
                     QGIGGSMLNPVAMSIITQVFTEKLERAKAIGLWGSVTGISLGMGPIIGGLIVSYFSWRY
                     VFFVNVPIIAAAIILTQKFVPESKVEKTAKNDFVGQALMILFLFSSIYSIIGLPRKGLF
                     APDILSTGIIGCLAIVIFFIYEYNIDNPLINPRFFLSIPFTSASFLAIFGFIIYNGYLF
                     LNTLYLQEMRGFSPLEAGLSTIPLAFVSFLVAPRAGEMVGRIGTKRPIMLCGISMLAVS
                     FLQLFVTKTTPMIILFVIYIFLGIGFGMLNSPITITAIEGMPLSQSGTAAAIAVTCKQI
                     GNSLGVALPSLLITKPITSSLTRTPFTNVWLLFGCCAIAIIFLSYLSNSPLAKKSLRRV
                     RFYF"
     gene            18517..20577
                     /locus_tag="HCH27_RS01235"
                     /old_locus_tag="HCH27_01235"
     CDS             18517..20577
                     /EC_number="2.7.8.-"
                     /GO_function="GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818414.1"
                     /locus_tag="HCH27_RS01235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCH27_01235"
                     /product="LTA synthase family protein"
                     /protein_id="WP_002381784.1"
                     /transl_table=11
                     /translation="MKKINNRLGLFLFFVLLFWAKTILAYYLDFSLGVKGSLQREILWF
                     NPVATTLLLFGFSLYFKKHKTFMLSLTLLNVLNTLILYLNIIFYREFTDFITIQSVFGF
                     SKVSEGISGDLLALMRVHDVFYWLDTLVLLVLVFYSFKQRRNHQVFMTKPINRRLAISI
                     SCLSVLLFSVNLFLSETDRPQLLQRIFDRNYIVKYLGLDAFTAIDGVKTAMSNEVRAEA
                     SSNELPEIMKFTKQNNLPLNPQYAGIAKGKNVFVIHLESFQQFLINMKVQGQEVTPFLN
                     QLYQNKATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTQLGSDNTFEAAPAVL
                     GQKGYTTAVFHGNVGSFWNRDHVYKNLGYQYFFDRSYFSKDDKMLGYGILDKQLLRESA
                     SELEHLQQPFYAKFLTVTNHTPYYTDDQNFPFPKLNTGNSIVDDYVRTAHYLDQSLEQF
                     FHYLQTTSLYNHSIFVIYGDHFGISDANNKDLCKAFNRDPKTWTNYDNAQLQRVPLMFY
                     MPGYTQGKIMHEYGGEIDVLPTLYHLLGVDDKNYIHFGTDLLSPQYKQVVPFRNGDFVT
                     PQFSYLGGEIYNNQGKKLDQVPADLKAEVTKDNDWVKKSLSLSDKLNQENLLRFYKPQG
                     FQEVQPKDYNYDVAFEKNKVLEVEKQKNAQSTSLFSQNGNQTTVQNYPTTP"
ORIGIN
        1 cggtgcagaa ggaaaagttg ttcctgtttg tcaaacacgt caaccagaag aaattaatgc
       61 tatttttgaa gaaatggaac aaggaaaaat tcgcgggcgc atggtcattg attttcgcag
      121 ttaattcagt tttttagtga ttcgttgatt taatctgaaa accatgaaag gatggatgga
      181 atgtttgcaa atcttactct ccgtgagaga cgactgctta agcaagcaca agtgggagcc
      241 acaattagct ttattctttt gtttgtacca attctgacaa tcagtctttt gcataccagt
      301 gacttattaa caaaatggct aggtatagtt ggggtaatta tggttttacc attgttgtat
      361 tttgcgtggc agattttaaa gatagcctat aaagatcaaa aagataatcc aacgcctcct
      421 ttgtgggttc cgaaagttta tgggattggg ctttcgatca atccctatca ccggtttgga
      481 aagttaattt ttatattgct agcggtactg atagttggca tcatcattag tttgcttttc
      541 acaccagcaa gtcaaatcaa tcactaaaaa acgagtaatt aacaccctct tgctgagcag
      601 gagggtgttg attattttta aatagactag atttgagcaa caggtattag atttttgaaa
      661 aagtctttct ttttctgatg tagatatttt cgcaataaaa tcatcattat aagtgaatta
      721 acaggtttat caagggttaa tacttgttaa tggtggctgc tataattaac cgttgacaaa
      781 cacaaaaaaa ccatcatacg ataattatgt tattaaaaaa gtgaaaaatt ggtagtcaac
      841 aaatgaaaac gcattcatta ggaaattgtg attctgtact aaaattttta tataagatga
      901 aaatggattg ttttcattta atattttatt gaaaggatgt gtttatgaag gggttactga
      961 aactgacttg cttacagtct cagtaaacta agaaaatcgt tatgactttt catctggata
     1021 aaactttgtt gggttttgga ttttgtgagt aattgtgtcg gaactattct atggggagca
     1081 attttaagga ggaaaaaatg aacaaagagt atcagggtaa aaaaatttac ggtaaaaagc
     1141 actggttgac atttttatta gtgagttcgt ctgtattggc gttccattcg tttgttacga
     1201 tggattctca agtggttgat gcgcaagagc aaacagcaaa agaagacgta gctgattcag
     1261 caacgtcagt cggagcaatt gtgtcgattg aaaaagcgga gaaaaacttt gtcattacgt
     1321 atgcttctgg aaaaaaagca caaattagta ttttaaacga ccatcttttt cgctatcatc
     1381 tagatccaac tggtaaattt gaagaatatc caacgccaaa tgatccaaag catgttgcga
     1441 aaattacggc taaaacaatg gccgactatg gtacacaagc ctttgagcaa acgaacgtca
     1501 cagattcagg aaaccaattt attctagaaa ataatggttt aaaaattatg ttcgaaaaag
     1561 aaagcgcttt aatgaaggtt ctagacaaga aaaagaatca agttatttta gaagaaaccg
     1621 ctccgctatc ctttaaaaat gataaagcta cacaaacgtt aaaacaaagt agtcaagaaa
     1681 attattttgg cggaggaact caaaatggtc gatttacgca taaaggaaca gctatccaga
     1741 ttgtcaatac gaacaattgg gtagatggcg gagtagcatc gcctaaccca ttttattggt
     1801 caactgcagg gtatggcgtt gttcgaaata catggaaacc tggcaactat gattttggaa
     1861 gtcatgatcc acaaacaacc acaacaacgc acgaaggaac agattttgat gctttttatt
     1921 tctttaatga ttcctcagca ggtattttaa aagattacta tgaattaacc gggaaaccag
     1981 ctttgatgcc agaatacggg ttctatgaag cacatttaaa cgcgtataat cgggactatt
     2041 gggtgaaagt agcagaagga accgcgggag cagttaagtt tgaagatggt aatttttaca
     2101 aagaatacca accaggcgat ctaggaaact taaatggcac gctagaatct ttaaatggag
     2161 aaaaagagaa ttatcaattt tctgcacgcg cagttattga tcgatataag aaaaacgata
     2221 tgccgttagg gtggttcttg ccaaatgacg gctacggtgc tggctatggc caaacagatt
     2281 cattggacgg cgatgtacaa aacttaaaag aatttacaga gtatgctcaa gccaatggtg
     2341 ttgaagtggg gctgtggact caatctaatc tccatccagc ggatcctaaa aatcctaaaa
     2401 aaggagaacg ggatattgcg aaagaagtta gtgtcgctgg tgttaaagct ctcaaaactg
     2461 acgtggcgtg ggtcgggtat gggtattcct ttggcttaaa tggtgtagaa gatgccgcga
     2521 atgtttttgt aaaagaaaca gacggcgcag ttcgtccgat gattgtttca ttagatggtt
     2581 gggccggaac ccaacggcac gctggtattt ggacaggcga tcaaacgggt ggtcaatggg
     2641 aatatattcg cttccatatc ccaacatata tcgggacttc gttgtctgga caaccaaatg
     2701 tcggttcaga tatggatgga atttttggtg gaaaaaataa agaggtcaac atacgtgatt
     2761 tccaatggaa aacttttaca ccagtgcagc taaatatgga cggctggggc tccaatccaa
     2821 aaacgccatt tgcttttgat caagaagcca cagatttgaa ccgagcgtat ctaaaattaa
     2881 aatcaatgat gatgccttat aactacagta ttgccaagga atcagttgat ggactaccaa
     2941 tggtacgggc catggcttta gaatttccta atgaaggaac agcatataca aaagattctc
     3001 aataccaata tatgtgggga cccaatttac tcgttgcccc aatttataat ggcaatcaag
     3061 atgaggcagg aaattcaatc cgtgatggaa tctacttacc agatgaaaaa caagtctggg
     3121 ttgacttatt tacaggcgaa aaatatcaag gtggccgtgt cttaaatgga gtaaaaacac
     3181 cgctgtggaa ggtgcccgtt ttcgtcaaag atggcagtat tatcccaatg acgaatccaa
     3241 acaacaatcc aaaggaaatc caacgagatc aacgatcttt cttgatttat ccaaatggca
     3301 ccacttcttt taatatgtat gaagatgacg gaatttcaac tagttatgaa gctgggcaat
     3361 cagctacgac taaaataaat agtcaaggcc ctaaatcaaa cgaaaaaggg gatctgaccg
     3421 ttactatcga accgacaaaa ggaagttata aaggctttgt tgatgaacga agcaccacgc
     3481 ttgatttgtt agcttcagaa gcgcccgaaa gcgtaacagc aatggtgggg ggaacagaag
     3541 taaccttgaa acaagcagct aataaagaag aatttttagc tggcacaaat ctatattatt
     3601 ttgataaaga attccaagtg aatcagtatc tttctgaagc ttcaggagaa aaactgaacc
     3661 aatcagcgtt atcagtaaaa ctagcgaaac aatctgtgac cgccaaagat gttcaaataa
     3721 ctgtcaaagg tttcattaat aaaggaacgg ttgacggagg aaatacaacg gtcgatgacc
     3781 agttaactat tccggcaaat gtcgcaatta atgaagaaaa aacaacgcca tcttctttga
     3841 ctcttcaatg ggatcaagta actgaggcaa caagctatga agtagagcgt gacgggactg
     3901 ttttcggaaa tattcaaacg aatacagcaa catttgatgg attttccttc ctaagcgaac
     3961 atacttttcg agttcgagct gttggaaaaa acggggtctc tgaatggagt gaaccgatta
     4021 aaggaaaaac acaagatgat ccctataaag aaacaattaa tcaagtcaaa gcaacaagta
     4081 atttaccaga acaaccaggc gcagaattga agaaattaac agataaagat ttgagtacag
     4141 gttggcatac gaactggtca accggcatcg ctaatccaag tgatggaaat ttcttgtctt
     4201 taaaatttga tcttggtgcg gagtatcaaa tggataaaat tgaatacttg ccaagagaca
     4261 atgccggcaa cggaaatatt ttgcagctcc agtatcggac gtcgaaagat ggagctaatt
     4321 ggacagagtt ttcagaacct attaactgga agcaagatgc tttgacaaaa accattgaaa
     4381 cgaaggacca agcgtatcgt tttgttgaaa tgaaggtatt aaaatcggtg ggtaattttg
     4441 gctctggtcg tgagatgctc ttttacaaac aaccaggaac agaaggaatc ttacatggag
     4501 acattactaa tgatggaacg attgatgaaa atgatgcaat gagttatcga aactatacag
     4561 gcttagaatc ggtggacagt gactttaatg gctatgttga aaaaggcgat ttaaataaaa
     4621 atggggtaat tgatgcttat gatatcagtt atgttttacg acaattagat ggtgggattg
     4681 agatacctga tgtcgaagaa attgcgggcg gtttatccct tgctgtggtt aatgaaaatg
     4741 gcaaggatac ctatcttcct ggcgatacat taacctttac acttaaaggt caagacttaa
     4801 aaaacattaa tgcgttaagc acaaaaatgt cctttgattc tagcaaattt gaacttgtcg
     4861 gtcagcctgc aaccacgaat aatacaaaac aaatggaaaa ttattctaaa tatcggaaac
     4921 attcaaatgg tgtggagaat ctatatttag ttcttagtaa tcaaggaaat aaacaattgt
     4981 taaatggttc aatggactta gttaccttta aagttaaagt aaaagaaaca actcgcgtaa
     5041 aacatgcgac aactgttgag cagccattac aatttgatat gagtcaaggc ttattagtag
     5101 gccaaggatt ccaacaagcc acactttcag atttttctgt aacagtgaag cccacagagc
     5161 tagtagataa agagttatta caagcgctaa ttacgctaaa tcaagcacgt gtcgaaaaag
     5221 aatatacgcc tgaaacttgg gcaattttta aaccagtctt agatgaggca gtagctgttc
     5281 tagccaatga acaagccaca cagacagacg tttctgctgc tgtggaaaac ctagaaaagg
     5341 ctgcaagtca gttggaaaaa atgcctgatg tagcgaataa agctgattta gaaaaagcaa
     5401 ttcaagaggg gctggcgaag aaaccaagtg atgggcaaga attcaccgaa gaaactaaaa
     5461 aagttctcga agactcatta gcagcagctc aaaaagtatt tgctcaagaa aaagttaccc
     5521 aagaagaaat agaccaagcg actaaaacat tgcgtgaagc aattgcacaa ttaaaagaac
     5581 aacccgtcgc cgttgataag gaaaccttaa aagagaaaat cgctcaagct cgaggaagaa
     5641 agccggaaga aggataccaa tttacaaaag aaacagaaaa acaattgcaa gaagcaattc
     5701 aagcggctga agcaatcgta gcgaaagaaa cggcgacaaa agaagaagtc tctgaggctc
     5761 taaacgcatt ggaaactgca atggctcaat taaaagaagt accccttgta aataaagacc
     5821 aattacaaga agtagtcaaa cgagcgcagc aagtcactcc aagtgaaggg caccaattta
     5881 ctgcctcctc gttacaagat ttacaaaaag cgcttttagc agctaaaaac acattggaaa
     5941 acccagcagc taatcaaaaa atgattgatg aagcggttgc tgaattaact agtgccatcg
     6001 atgggttaca agaagaagcg ttagttacag ataaaaaagc cttagaggca atgatagcca
     6061 aagcaaaagc aatcaaacca tctgccggaa aagaatttac cagcgagtct aaagcgcgct
     6121 taactgaagc aattgaccaa gcggaaggga ttttagctga taaaaatgct cggcaagaac
     6181 aaattgatac tgctgaaaaa aatgtaaaaa ctgctttgga tagtttagag gaacaagtcc
     6241 ttcaaacgga taaaacaaag ttgaaagaac tgcttcaaaa agcagaaacc ttgaaaccta
     6301 aagcaggaaa acaatttaca aaagcatcgc aagaagcttt agctgaagca attaaacaag
     6361 caaaagcact tgttgaggac ccaaatgcga cgcaagaagc tgtggacaaa tgtttaagta
     6421 ttttagcaca agccattgaa gcaatggcag aagagccaat cagtagcaac tctacaggca
     6481 acaatggaaa tcatggcacg gttagcggga ctggtggcgt aaccagtcca ggaaagggca
     6541 cagcagcagg tggaacgaca acgaaaacca ccacttctgg tactttgcca aaagcaaatg
     6601 aagttgtttc gccaatctgg tcaattagtg gttttctatt gatagtaagt attggtttag
     6661 gaaaattatt cttcaaaaat aaaaaagacc aaaattagcg tatcattaac ttacaaggcg
     6721 catttgtaca aaaacagtgt gcaaatgcgc gagtatgtaa taggagagga tttttacgaa
     6781 tgaaaagaaa gaaaatcaag aatcaactac ttgttttaac aagtattctc gttcttctga
     6841 gtttggaagt cgctccagtt gttacgttgg ctgcagaatt accaagtagc tcgctacaaa
     6901 cagctctgtc ctcggaaacg acaattactt cggaagaaaa agtaattgaa acaaccgaaa
     6961 caaccgaaac aactgttgct acatcgacaa cgtccactag tagttcgtca tctgaatcaa
     7021 atagttctac cgatacgact accgaatcaa cgagtcattc aacggcagaa acgacaacca
     7081 caaatactag ttctgaaaca aaaaaagaac ctacagagtc aacggtatca tcagaaatca
     7141 cgcagcctgt tgaacaatcg caaccaacac aagttcaacc accacaagtt cctgtgacca
     7201 agcaagaacc cgaagaacct attcaagttc ctgaagccac caataacgtt gttgaagaaa
     7261 atcaggctgt atcgttaaat ccgtccctca aagtagatga aatagcttct tcaaatttaa
     7321 aaggctacga acttccactg ttgtcgtctt ttggcgaaaa gaaacgggca gttgtggtgg
     7381 ctgaagcatt aagacacgtt gggaaaacaa aaaaagaatt taatttgacg gagcaagcat
     7441 tgaccagtag tttccttgcc caaaaaattt accaacaatt atttaaaata gacattggca
     7501 gtacaccgca agaacaaata acatttggta aagttcgttc tatagaggag gcagaacctg
     7561 gtgatctgat tttttggcaa acagctgaag gaaaaacgct tcaaaatggt gtttatcttg
     7621 gccaaggaaa atatttaatt gccgcagcgg aagcggattc taaagaggaa ccagaagtaa
     7681 ttggtcagtt agagaatatc tatactgcca aacaacaacc agactcaaaa gaggaaaaac
     7741 gcttggtagt gactaatcct tttaaggagt tcacattaac ggagtatggc aaagaagttt
     7801 tagcaacata tggtgcgtca tttgaaatgc agaagtcaga acaaaccaca gcgtttatta
     7861 aaaaaattgg tgagacagcc agagaacttg gcgaaaaata cgatgttttt gcatcagtga
     7921 tgatagctca ggctatttta gaaagtggct ctggcgaaag tcaactagcc aaagaacctt
     7981 attataatct ctttggcgtg agaggttcgt tccaaggaaa tagtgttagc ttttcaacaa
     8041 aagaggctga tcagaggggc caactttata caatttccgc tgggtttcgc gattacggtg
     8101 gctataatga ctcactgcaa gattatgttc aattactccg acaagggatt gacggaaatc
     8161 aagattttta taaaccagcg tggcgttcgg aagccaaaaa ctaccttcaa gcaacgcgtt
     8221 ttttaacagg taaatatgca acagacaagc aatacgataa taaattaaat tcgttaattg
     8281 cggtttataa cttaacgcaa tttgatttac caaaaacagt ggacggtttg attatccaat
     8341 ctaaaaataa gctgtcggaa gcggaacagc aacaaatgca ttttccagtc tatgatggga
     8401 tcaattataa tcgaagtggt agctatcccg tgggccagtg tacgtggtat gtctataatc
     8461 gtttcaaaca gttgggcact tctgtggatg aatttatggg aaatggcagt gactggggaa
     8521 gaaaaggacg tgcattaggc tatcaagtta gttcattgcc taaagctggt cgtgcgatta
     8581 gtttccaacc aggagttgct ggcgcagata atcaatatgg acatgttgct tttgttgaag
     8641 cggtcacttc tgatggaatc attatttctg aaagcaatgt catcaatgat caaacgattt
     8701 cgtatcgtgt cttacctaat gtaattgctt atagtagtgg cgtgacctac attggcgctt
     8761 aagcgactca ttcaacagga agaagtgtgg atgtttaagt aaaaaattag cacaataatc
     8821 catgtgtgaa ctaacaaatt aaaataacca gaatcgacca atgaattgat tttgtcatgt
     8881 ataaaatgac aagaacagtt tggcggttga ttctttgttt ttcatcaacg aagagacaat
     8941 agtcctcatg attcgttaga aaaagatgaa tagtccaaag tactctttct tgatgctttg
     9001 ttattttatg tttttgtttg caggaaacta ctgaaatcgc tatactaaat ttatgtgaat
     9061 atactaacag acaatcaatt ttttatctct tttattggtg tataaattga ttgaacatta
     9121 taatcatttg tcttttgttg tgtaatttta tatcttcttg agaaagggat gagtgaacaa
     9181 atggctattt atatattaga agaccaaatt attcaagcaa aagcgttaga agcattggta
     9241 acaaatatcc tacattcaag aaatatttat aatgaaacaa tccatttatt ttcacgttca
     9301 gatgaattat tacaagtggc acaccaggac gcccaactaa atatcttttt cctagatatt
     9361 caaatgaaca atcatatcca ggcaggattt gaggttgcta aagaaattag aaaaacagac
     9421 agtgaaagtc tgattgtatt tatttctact cacacagaat tagttttaac ttcctacaaa
     9481 tatatggttt cagctcttca atttattcaa aagaacgtgg attttctaga tttccgaaaa
     9541 gaagttgaaa catgtgtcga cgcatatatt caacaaaaag aaaatataaa gacaaaaagt
     9601 gaatatatta tcattaactt aaaagcgtct tctataaaaa tggatattaa tgatatttat
     9661 ttctttcaaa cggaatacga tcatcgtgtg tcaatggttg gtaaaaattt taaaagagaa
     9721 ttttatggaa cgttatcaaa aattgaacaa ctgcatccag atttgattag agtccatcaa
     9781 tcaatcatca tcaataaaaa atatgcgtca aaattaaatt ataaaacgca tcttttaact
     9841 atgagagacg gaacggaagt accagtctca agaagatatt atactcaggt caaggcacta
     9901 tttcttactt agggagggat aatgactaat taaggaatta tctatctatt agtcgctata
     9961 ttcgttataa tttagtgcaa tacttgaaga ggagggcgat atgctaatcg attggattct
    10021 aaaaaatatt atggatatgg atcaggaaga tcaatcagga aaaacacaat ggacaaagta
    10081 ttatctaacc gtttattttt ctggcttatt taattttctg atgattctga ttttatcagt
    10141 tttatttggg acgttaagcg aaacctttat tgtatacgtc gtactgattt ttttacggcc
    10201 tgtcgcaggt ggctggcatg caaaaactaa atggctctgt cgtctagaaa gcattgttat
    10261 ctatgtcgcc ataccatttg tattgaaaaa ttcttctgtg agcttaccgt ttatttataa
    10321 aattctattg atttgcctct tagtcgtatt attttattgg tatgcgccac aaggaacagc
    10381 aattgaacct gttcagccat ctgatttaaa cgtgctcaaa aagcaaagcc ttataagggt
    10441 gtgtttactt attttatgta gtctgtttgt caaagaaaag attgcttcag taatactcta
    10501 cggtctcgtc atccaaggtc tgatgatact ccctgtaaca aaaaatttaa ttgaaggaag
    10561 tgtttttatg aaatttggta aaaaaataat taaaaatgtt attgaaaaaa gagttgcaaa
    10621 agttagtgat ggtgtgggaa ctaagcctag attaaatcaa aattcgccca atatatttgg
    10681 acaatggatg ggacaaactg aaaaacctaa aaagaatatt gaaaaatgat tttgttgtta
    10741 ttagctacta acgttttgct tgtatctagc tttatcgttt ttgtcttttt acgagtaaca
    10801 ttgatcaaga ttgaatgtaa aataccgtta ctttcattgc ttattgtcat taatctttgt
    10861 tcgtttgcgg cattaatgtt gggctactct tggttgattt atgcgctgac agtcgttatt
    10921 tttacaggat ttttactcat tcacaaaaag aggttctcaa tttttaaagc gatattttta
    10981 tccgttttta cattgcttat ggtttcgttt atcaattaca cggagcaaac gattttaagt
    11041 gttttttttc aacagattta tcaaaataaa ttattatgga ttgcctcaaa tgttcttctg
    11101 ttgcttataa atatctggat tgctttaaaa attcccaata gtgttttttt aagattaaat
    11161 cgtgtgttag aaaatagtcg aatttttttt ggttgtttac ttttattgtt gattctgttg
    11221 ttactttttg tgtttttgat ttcgccagag atttcacctg actttatgcg aggatttgtc
    11281 acggtaaata gttctaaatt ggagttatta ataagtgtag gtttattttt aattctgatt
    11341 ggcttagtca ttgaagctta tttggaagaa caacgtatca acactcaatt attgaataat
    11401 ttaacgattt atactgaaaa aatagaatcc attaacgaag agctagcgat gtttcgtcat
    11461 gattataaaa atttattgta tagtttacaa attgctattt catacgaaga tattctggaa
    11521 atcaaaagaa tttatgaaga aacgattgca ccaaccaaaa aaattattga taatgaagaa
    11581 tttgaactta tgaagttaaa tcgtttgaaa aatatggaac ttaaggcact cataagtatg
    11641 aaaattaata ccgcaaagca agcaaaacta aaagtgattg ttgatgtgcc agaagtattt
    11701 attctcgata catcaatcga cttagtggtt gtgattcgtt tgttggctat tctattagat
    11761 aacgcgattg aaaacagtgc aaaatcagaa ttgaaaatgt tcgctatctc aattttcaat
    11821 aaaaatgaaa cacaagagtt tgtgataaca aatagtgtcc aagccgaatt tgattttagg
    11881 gttatgaaga aaaccaaatt tagttctaaa agtaacccag aagagcacgg ttggggattg
    11941 ttatatgtaa aagaaattgt tgatttttca gatcaatttg acttgcaaac gtccttcaat
    12001 gaaggagcgg tcactcaaca tttaattatt gaaaaaaatc ataacagtaa aaaagttgtt
    12061 aacgaatgaa tttgttaaca acttttttgc tatggtattg agttatgagg ggcaatacag
    12121 ggaaaaatgt cggctgatta aggaatttag atagtgccgg ttaggtagtt gtctataatg
    12181 aaaatagcaa caaatattta cgcagggaaa ggggcggtcg tttaacggga aaaattaggg
    12241 aggataaagc aatacttttg ttgggaaaag aaataaaagg aaactgggga aggagttact
    12301 gttgattcag ataatgtgat tattcattta gataaaaacg gtgcagtaac aagtgttaca
    12361 ggaaatccag ttgatcaagt agtgaaaatt caatcggttg atgcaatcgg tgaagaagga
    12421 gttaaaaaaa ttattgcttc tgataatcca gaaactaaag atattgtctt tttagctatt
    12481 gacaaacgtg taaataatga agggcaatta ttttataaag tcagagtaac ttcttcacca
    12541 actggtgacc ccgtatcatt ggtttataaa gtgaacgcta cagatggaac aattatggaa
    12601 aaacaagatt taacggaaca tgtcggtagt gaagtaacgt taaaaaactc ttttcaagta
    12661 acgtttaatg taccagttga aaaaagcaat acgggaattg ctttacacgg aacggataac
    12721 acaggggttt accatgcagt agttgatggc aaaaataatt attctattat tcaagcgcca
    12781 tcactagcga cattaaatca gaatgctgtt gacgcctata cgcatggaaa atttgtgaaa
    12841 acatattatg aagatcattt ccaacgacac agtattgatg atcgagggat gcccatcttg
    12901 tcagttgttg atgaacaaca tccagatgct tatgacaatg ctttttggga tggaaaagca
    12961 atgcgttatg gtgaaacaag tacaccaaca ggaaaaacgt atgcttcctc tttagatgta
    13021 gttggtcatg aaatgacaca tggtgtgacg gaacatactg ccggtttaga atatttagga
    13081 caatcaggtg ccttgaatga atcttattct gatttgatgg gttatattat ttcgggtgca
    13141 tctaatccag aaattggtgc ggatactcag agtgttgacc gaaaaacagg tattcgaaat
    13201 ttacaaacgc caagtaaaca cggacaacca gaaaccatgg ctcaatacga cgatcgagca
    13261 cggtataaag gaacgcctta ttatgatcaa ggcggtgttc attataatag tggaattatt
    13321 aatcggattg gttacaccat tatccagaac ttaggcattg aaaaagcaca gactattttc
    13381 tacagctcgt tagtaaatta cttaacacct aaagcacaat tcagtgatgc tcgtgatgcg
    13441 atgcttgctg ctgcaaaagt tcaatatggc gatgaagcag cttcagtggt gtcagcagcc
    13501 tttaactctg ctggaatcgg agctaaagag gatattcaag taaaccaacc aagtgaatct
    13561 gttctggtca atgaatgaaa aaaattcccc aattaaataa aaatggataa ggagtggcat
    13621 aatcttatga aaaagttctc catacgaaaa attagtgctg gttttttgtt tctgatttta
    13681 gtaactttga tcgccggttt tagcttgtct gcaaatgcag aagagtatat cgttcctgcc
    13741 gaaagtcatt cacgacaaaa aagatcgtta ctggaccctg aagacagaag acaagaagtg
    13801 gcagatacaa ccgaagcgcc ttttgcgtca atcggaagaa tcatttcccc tgccagtaaa
    13861 ccaggctata tttctttagg aacaggcttt gttgttggaa ctaatacaat tgtcaccaat
    13921 aatcatgtgg ctgaaagttt taagaatgcc aaagtattaa atccgaatgc caaagatgat
    13981 gcttggtttt atccaggtcg agatggcagt gcgacaccat ttggcaaatt caaagtgatt
    14041 gatgtagctt tttccccgaa tgcggatatt gcggtagtga ctgtcggcaa acaaaacgat
    14101 cgtccagatg gcccagagtt gggagaaatt ttaacgccat ttgttttgaa aaagtttgaa
    14161 tcttcagata cccatgtcac aatatcaggc tatccaggtg agaaaaacca cacgcaatgg
    14221 tctcatgaaa atgatttgtt tacatctaac tttacagact tagaaaatcc attactattt
    14281 tatgatatcg atacaacagg tggtcaatct ggttcaccaa tctataatgc tcggtttgaa
    14341 gtagttggtg ttcattccaa tggtggcatt aagcaaacag gaaatcatgg tcaaagacta
    14401 aatgaagtga attataactt tattgtcaat cgagtgaatg aagaagaaaa taaacgttta
    14461 tccgctgtgc cagtagcgta aagaaccata aaaatactcg gaatttccag aatcacggga
    14521 aatttcgagt atttttgttt tttaagatag tgtaagttac gatataagta ccttgtaatt
    14581 atcggttaaa agcatataat agtgctgaaa agaaggcaaa taatccgatt aagttagtat
    14641 ttctaaacca tcataactcg taatgatttc atgcttttga gataaagccg ccatctccgc
    14701 ataagacaag ccgccattgt gagaaaaatg gttggcaacg taaatcgttt tatctgtgac
    14761 gagttttctt tcttttaatt ctgcaattaa ttttaaattt tcttcccaat tcatatgact
    14821 attgccagtt tcgtttgttt gactagtaca atctaaagaa acaatactaa gaagtaagtg
    14881 gctcttttca agatattcaa acatttctga cgtaaagtaa cctgtatcat gggtggataa
    14941 aaaagcatct ttaccatcgt ctataacgaa aataaaacaa tcttcactga aatttccatg
    15001 ttgtgcagga aggggataaa tcgtatagtt cgcaatagta aaacatttgt agggctgaat
    15061 aacctgataa tgtaatcggg tatcgtcttg acgaccttct aaatcaaagg cgcgctgata
    15121 aaacgtctta actgcttcat ttccgtaaac agttaaacga ttgggaatat ctaatgcata
    15181 accagacatt cgataagcca aatcctcgcc gtaaaaatga tcggaatgcc aatgagagat
    15241 tagtaaagag ttaaagtctg caaaacacaa gtcatactgc aacaagtgta aataagaatc
    15301 ccctggaaaa tcaatcagca ggatatcatc aataattaat tgagtttgtg tacgaatttc
    15361 tcgtcctttt ttctctcgag cgtatttaca taatttacaa ttacaaaaaa ttcctggaat
    15421 ccgttctgct gcacccgtac ctaaatagtt gattttcatt gggagtacct aacctttctt
    15481 tgacattctt catacttagt agataacgct gaaccttaaa gtaaatgggc atggaaactg
    15541 aaacatttgt tgtgtccatc ttaccttttt tagagaagaa ttgaaatgga atttcttaag
    15601 taaatttgcg taaaatagaa aaagctagac gccttttttg ataagccgaa tttactgcga
    15661 atgctcgtac tatttccgaa aaaaagtttt accaaaggca ctttcatatc catctgctat
    15721 tagttataga aaaataacaa gatggacata gcttattaga gcaaaaataa ctacgctatt
    15781 gataaagagt gaggcgtgtt ttgaacaggt tgcgtgggca aatgaatttg aataaaaggg
    15841 atgacaaaag atgactattg aaaaattaga atatttttat accattgcaa aatataatag
    15901 tatttccaaa gcagcctctg aactccatgt tagtaaatca accttaagtg cgtcattaaa
    15961 agatttggaa agtgaattgg gccatttact ttttgaccgt aacgggaatt ctttgacgtt
    16021 aaattcctat ggtgataaaa tcgtccaatc tgtatatatc attttaaacg aggctaagaa
    16081 aatgaaactt aatttgcatg aaatgattga gaatccagtg atgcgtttag gttttggaaa
    16141 tacgtccttg atgtataaag tgacagaaaa tgaggaccaa ctgaatcgtt tttgggaatg
    16201 ttaccatggt tcatcgtttg agttattgaa taaattggag aatcatgagt tggattttgt
    16261 gattacttct gcagatgtga atagtccagt tttaaagaaa gaacaattaa ttgaactaaa
    16321 aatgtatctt tgtgtttcaa gagaaattaa acaagaaatt gaggaagaag gcttcacctg
    16381 tttgaccaac tatccatttc tatttttgcc tcatcactta gatcatttag aagctaccaa
    16441 gagtgtgcta gaaatgttgc aattaacaag tccgcttgtt tgttgctatg atacgttgat
    16501 gcttactcgt ctcattgaaa aaagtaaagg agtttatgcg gtgatctctc ttcggaaaga
    16561 acaactgcag gaaattgatt ctaaactctt ctttttaccg attgaacgaa aacaaaaatt
    16621 ttatttatac cgaaatgttt ccagttcggt ttttgtccaa cctggtcaaa tcaaggcaac
    16681 tttacaaaag ttgctagaga cttagccatt aaaattactt tggaatagtt acccaaaata
    16741 gctttattcg cttagattta cttctgctgc ggataaggct atttttgttg ttcattgttt
    16801 aaaagagatg ctcaattatg ggactgttcg atatttctga acggaatgtt caattattta
    16861 ataacttaag aatcgataaa tcgtatctat tacggttcaa tttaaagggg ttctggtgta
    16921 tagtttggct atctaaagat ttactttttt aagtataaaa taactaaatg gcaaggaata
    16981 taaagaactg ttgatacgtg agtacttgcg ataactgtgt cgttagaagt ttaagtgtca
    17041 ttgttgcttt tcctgtttta tgtagttgct agaaaggact gtttttatga tcgatagaaa
    17101 aaaagttatt ttgtatactt gttgcatgag tttgtttgta gtgaccatgg atgttacggt
    17161 tgtcaatgtg gcgttaccat ccattcaaag tgattttcac acgaatctgt ctacattaca
    17221 gtgggtaaca gatggctaca ctttaatggt agcatcctta ttattgttat ctgggtctac
    17281 agcagaccga attggccgta aacgagtcct tcaattgggc ttagcctgtt ttggtttagc
    17341 gtctttccta tgtgggattt cgcaaacccc agggcaattg attgcgtttc gcatgttgca
    17401 agggattggt ggttctatgt taaatccggt agcaatgtct attattacac aggtatttac
    17461 cgaaaagtta gaacgagcga aagcaattgg cttgtggggc tctgttacag ggatttcctt
    17521 aggcatgggc ccaattattg gcggactgat tgtttcttat tttagctggc ggtatgtctt
    17581 ttttgtaaat gtaccgatca ttgctgcggc aatcatcctt acacaaaagt ttgtacctga
    17641 gtcaaaagta gagaaaacgg cgaaaaatga ttttgttggt caagcattga tgattctttt
    17701 tctatttagt tctatctatt ccattatcgg actacctaga aaagggcttt tcgcgccaga
    17761 tattttaagt actgggataa tcggctgctt agccattgtt attttcttta tttatgaata
    17821 taacattgac aatccgttaa tcaatccgcg cttcttttta tctattccat ttacatcggc
    17881 ttctttttta gctatttttg gctttatcat atataacggc tatttatttt taaacacgct
    17941 atatttgcaa gagatgagag gcttcagccc gttggaagct ggcttatcaa ccattccttt
    18001 ggcttttgtt agttttcttg ttgccccgag agccggcgaa atggtaggga gaatagggac
    18061 gaaacgtcct attatgcttt gtggtatttc aatgttggct gttagctttt tacaattatt
    18121 tgtaactaaa acaacgccta tgattatttt atttgttatt tatatctttt taggcattgg
    18181 gtttgggatg ttaaattcgc cgattaccat tacagcgatt gaaggaatgc cactttctca
    18241 gtcaggaacg gcagcagcca ttgcggtgac atgtaagcaa attggcaatt ctttaggggt
    18301 tgcgctacca agtcttttaa ttacaaagcc tattactagt tcgcttactc gaacaccttt
    18361 tacaaacgta tggcttttat ttggatgttg cgccattgcg attatctttt taagttattt
    18421 atccaattct ccgctagcga aaaagtcatt gcgcagagtt aggttttatt tttaacaaat
    18481 caagattttt ttgaagggaa aggaagacaa acttagatga aaaaaataaa taaccgatta
    18541 ggactctttt tattttttgt tttactcttt tgggcaaaaa cgattctggc gtattaccta
    18601 gatttctcat tgggtgttaa gggcagtctc cagcgagaaa ttttatggtt caatccagtt
    18661 gctactacgc tgttattatt tggttttagt ttgtatttta aaaagcataa aacatttatg
    18721 cttagcttaa ctcttttaaa tgttttaaat acattaattt tatatttgaa tattattttt
    18781 tatcgtgaat tcactgactt tattaccatc caatctgttt ttggcttttc aaaagtttca
    18841 gaaggaattt ctggtgattt acttgcctta atgagggtcc acgatgtctt ttattggcta
    18901 gatactttag tgctgctcgt tttggtattt tactctttta aacagcgacg gaatcatcaa
    18961 gtctttatga ctaaacctat caacagaaga ttggcgatct caattagttg tttaagtgtc
    19021 ttgctctttt cggttaactt atttttaagc gaaaccgatc gtcctcaatt gcttcaaaga
    19081 atttttgatc gtaattatat tgtaaaatat cttggcttgg atgcattcac agccatcgat
    19141 ggtgtcaaaa cagccatgtc aaatgaggta agagcagaag ctagttcaaa cgaattacct
    19201 gaaatcatga aatttaccaa gcaaaacaat ttaccgctta atccgcagta tgccgggatt
    19261 gccaaaggaa aaaatgtatt tgtgattcat ttagaaagtt ttcaacaatt tttgattaat
    19321 atgaaagtcc aaggccaaga ggttacaccg tttttaaatc agctctatca aaacaaagcg
    19381 acaatcagtt ttgataattt ctttcatgaa gtagggcaag gaaaaacgag tgatgcggag
    19441 aatatgttag aaacaggaac attcgggctc ccgcaaggct ctctttttac acaattagga
    19501 tcagacaata ctttcgaagc ggctcccgct gttttaggac aaaaaggcta tacaaccgct
    19561 gttttccatg ggaatgtggg gagtttttgg aatcgggacc atgtgtataa aaacttaggt
    19621 tatcaatact tttttgatcg gtcatatttt tcaaaagatg acaaaatgct aggttacggt
    19681 attttagata aacaattgtt gcgtgaatca gctagtgagc ttgagcattt acagcagcca
    19741 ttctatgcga agtttttaac tgtgaccaat catacccctt attacacgga tgaccaaaac
    19801 ttcccgtttc ctaaattgaa cacaggcaat tcgattgtgg atgattatgt tcgaacagcg
    19861 cactatttgg atcaatcgct agaacaattt ttccattact tgcaaacaac aagtttatac
    19921 aatcattcta tttttgttat ttatggtgac cacttcggta tttcagatgc aaacaacaag
    19981 gacttatgta aagctttcaa ccgcgatcca aaaacgtgga caaactatga caatgcgcaa
    20041 ttgcaaagag tacctttgat gttttacatg ccaggctata cgcaagggaa aatcatgcac
    20101 gaatatggtg gggaaattga cgttttaccg acactgtacc atcttttagg tgtcgatgat
    20161 aaaaattata tccattttgg cacggacctt ttatctcctc aatataaaca agtggttcct
    20221 tttagaaatg gcgattttgt aacgcctcaa ttttcgtatc taggtgggga aatctacaat
    20281 aatcaaggga aaaaattaga ccaagttcca gcagatttaa aagcagaagt cacgaaagat
    20341 aacgattggg tgaaaaaatc gttaagttta tctgacaaat taaatcagga aaacctttta
    20401 cgtttttata aaccacaagg attccaagaa gtgcaaccaa aggactacaa ctatgatgta
    20461 gcttttgaaa aaaacaaagt cttagaagta gaaaaacaaa aaaatgcaca atcaactagc
    20521 ttgtttagtc aaaatggcaa tcaaacgaca gtccaaaatt atccgactac gccatagatc
    20581 atttaagaag gataattttt ggaagggaaa gagaatcata tatgaagtga aggaggtcag
    20641 gacagaaccg tttattttcg aaggaattgg ttctgttctc gccatttatc aggttttgag
    20701 cctggaacaa aaatccaaag tgatttttg
//
