LOCUS       NZ_AP024565            27348 bp    DNA     circular CON 26-SEP-2024
DEFINITION  Microcystis aeruginosa strain NIES-88 chromosome, complete genome.
ACCESSION   NZ_AP024565
VERSION     NZ_AP024565.1
KEYWORDS    RefSeq.
SOURCE      Microcystis aeruginosa
  ORGANISM  Microcystis aeruginosa
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Chroococcales; Microcystaceae; Microcystis.
REFERENCE   1
  AUTHORS   Phan,C.-S., Matsuda,K., Balloo,N., Wakimoto,T. and Okino,T.
  TITLE     Argicyclamide A-C unveil enzymatic basis for guanidine
            bis-prenylation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5478552)
  AUTHORS   Matsuda,K. and Wakimoto,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2021) Contact:Kenichi Matsuda Hokkaido University,
            Graduate school of phrmaceutical sciences; Kita 12, Nishi 6,
            Kita-ku, Sapporo, Hokkaido 060-0812, Japan URL
            :https://www.pharm.hokudai.ac.jp/tennen/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 87x
            Sequencing Technology             :: DNBSEQ; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019704275.1-RS_2024_09_26
            Annotation Date                   :: 09/26/2024 11:16:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,602
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,071
            CDSs (with protein)               :: 5,071
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 110 of 479
            Pseudo Genes (incomplete)         :: 380 of 479
            Pseudo Genes (internal stop)      :: 101 of 479
            Pseudo Genes (multiple problems)  :: 91 of 479
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 14:54:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4946412
            Orig. end                         :: 4973760
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024565.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated at DFAST https://dfast.nig.ac.jp/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..27348
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyanobactin"
                     /region_number="11"
                     /rules="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /tool="antismash"
     cand_cluster    1..27348
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /kind="single"
                     /product="cyanobactin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..27348
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:17348](-)"
                     /cutoff="20000"
                     /detection_rule="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /neighbourhood="10000"
                     /product="cyanobactin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..17348)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /neighbourhood="10000"
                     /product="cyanobactin"
                     /protocluster_number="1"
     misc_feature    complement(208..222)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.92"
                     /tool="antismash"
     misc_feature    complement(681..694)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 21.21"
                     /tool="antismash"
     gene            complement(748..1287)
                     /locus_tag="LC603172_RS24855"
                     /old_locus_tag="MAN88_48300"
     CDS             complement(748..1287)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012267582.1"
                     /locus_tag="LC603172_RS24855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48300"
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_061432700.1"
                     /transl_table=11
                     /translation="MKPKLLTAFCLLLITVFSCFHPAIAGAVTPTGGAPAKITLVEPAA
                     ADNLQKLQETNACVGCDFNGISLKDLNLSSANLEGANLSQADLERTNLQGANLKGTDLR
                     GADLGKTLLAGADLSKANLLGADLEKANLQGANLTNANLQKADLEKANLTNARLDGANL
                     QDADGEGAIGVDPNLF"
     misc_feature    1353..1366
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.72"
                     /tool="antismash"
     gene            1662..2357
                     /locus_tag="LC603172_RS24860"
                     /old_locus_tag="MAN88_48310"
     CDS             1662..2357
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002767335.1"
                     /locus_tag="LC603172_RS24860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48310"
                     /product="TerC family protein"
                     /protein_id="WP_061432701.1"
                     /transl_table=11
                     /translation="MSPQILDIAENLSLTTCGTILTLAVLETILSADNAVALAALVREL
                     PDPRQQRQALNWGLAGAFVLRVALLVSASWTVKFWQVEVLGAIYLIWLAGKHFWQKFLN
                     IEIEDENFATNLAIESFGRVIVLIAFTDLAFSLDSVTTAIALADRFWILLTGGILGMLA
                     LRFLTGLFVRWLAEFVYLQDAAYLTVLAVGGRLLLKACQPTYLPPEWMVLMMVAASLSW
                     GFSRRVVVE"
     gene            2496..2735
                     /locus_tag="LC603172_RS24865"
     CDS             2496..2735
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002767334.1"
                     /locus_tag="LC603172_RS24865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_223210634.1"
                     /transl_table=11
                     /translation="MLDTISTLSPKLTAIETKGNPLPKVVSCSSTCRYCRFYKFQGRGN
                     GYCQRLGVAVHSHWRACPLICPPFAPSWERSENG"
     gene            complement(2813..3211)
                     /locus_tag="LC603172_RS24870"
                     /old_locus_tag="MAN88_48320"
     CDS             complement(2813..3211)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012267579.1"
                     /locus_tag="LC603172_RS24870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48320"
                     /product="YidH family protein"
                     /protein_id="WP_002762678.1"
                     /transl_table=11
                     /translation="MLFKSQKTKQKFNPDYWRDHSANERTYLSWMRASIALMGFGLVIL
                     RLRTVSSSSLGLGWLLGFVFAMVGLLTVVIATRRYFLIRRAIDSNSYEPAQVWIICFSI
                     AIACLGSGILYFVATSPDSGGVESIGFD"
     gene            complement(3283..4089)
                     /locus_tag="LC603172_RS24875"
                     /old_locus_tag="MAN88_48330"
     CDS             complement(3283..4089)
                     /GO_component="GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA]"
                     /GO_component="GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004163980.1"
                     /locus_tag="LC603172_RS24875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48330"
                     /product="prohibitin family protein"
                     /protein_id="WP_061432702.1"
                     /transl_table=11
                     /translation="MLKIRLLARPSNLFFLLLILAIIFNPFVIVNAGERGVLMVFGQVQ
                     DKILNEGIHGIIPVVNTVKKLSVRIQKQQIAAEASSKDLQEVFTDVAMNWHILASEVNT
                     IFQQIGDEAAVIERVIDPAVEEILKAVMAKYTAEELITKREEVKGEVDIRLTERMKNYH
                     IGVDDISLVHVNFSDRFTDAVEAKQIAEQEAKKAGFMVLKALKESEVKINLAKGEAAAH
                     RILQDSLSPEVLQNKAIERWDGKLPLFMDDNLLKSLELAKDKRKTK"
     gene            4296..4445
                     /locus_tag="LC603172_RS24880"
     CDS             4296..4445
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS24880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_221275413.1"
                     /transl_table=11
                     /translation="MSIYSYHSRRLNVLLIMNQNDYLVSFSQKLDQKSIYLSKKGIGII
                     QKQL"
     misc_feature    complement(4550..4567)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.23"
                     /tool="antismash"
     misc_feature    4551..4569
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 24.21"
                     /tool="antismash"
     gene            4729..6027
                     /locus_tag="LC603172_RS24885"
                     /old_locus_tag="MAN88_48340"
     CDS             4729..6027
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0019346 - transsulfuration [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (63-225). E-value:
                     3.3e-10. Score: 31.5. Matches aSDomain:
                     nrpspksdomains_LC603172_RS24885_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1168: O-succinylhomoserine sulfhydrylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002767323.1"
                     /locus_tag="LC603172_RS24885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48340"
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="WP_061432703.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.8e-14,
                     bitscore: 52.1, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 3.1e-08, bitscore:
                     31.5, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 2.8e-06, bitscore:
                     24.9, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDRYRFETLQVHAGQEPAPGTNARAVPIYQTTSYVFNDADHGAR
                     LFALEEFGNIYTRIMNPTTDVFEKRIAALEGGAAALATSSGQAAQFLAIANIAGAGDNI
                     VSTSYLYGGTYNQFKVSLPRLGINVKFVDGDEAEAFRAAIDDRTKALYVETIGNPRFNI
                     PDFAALAHIAHENGIPLIVDNTFGCGGYLCRPIEQGADIVVQSATKWIGGHGTSIGGVI
                     VDSGKFDWGNGKFPQFTEPAPGYHGLNFYEVFGPSGPLGNIAFIIRARVEGLRDFGPAL
                     SPFNAFLLLQGLETLSLRVDRHVSNALALANWLKEQPQVEWVDYPGLPEHPYHERAKKY
                     LKHGFGGVLAFGIRGGFEAGRNFINRVKLASHLANVGDAKTLVIHPASTTHQQLNEEEQ
                     RSAGVTPELIRVSVGLEHIDDIIEDFQQTFQSL"
     aSDomain        4918..5403
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LC603172_RS24885_Aminotran_1_2.1
                     "
                     /evalue="3.30E-10"
                     /label="LC603172_RS24885_Aminotran_1_2.1"
                     /locus_tag="LC603172_RS24885"
                     /protein_end="225"
                     /protein_start="63"
                     /score="31.5"
                     /tool="antismash"
                     /translation="TDVFEKRIAALEGGAAALATSSGQAAQFLAIANIAGAGDNIVSTS
                     YLYGGTYNQFKVSLPRLGINVKFVDGDEAEAFRAAIDDRTKALYVETIGNPRFNIPDFA
                     ALAHIAHENGIPLIVDNTFGCGGYLCRPIEQGADIVVQSATKWIGGHGTSIGGVIVDS"
     gene            6043..7095
                     /gene="metX"
                     /locus_tag="LC603172_RS24890"
                     /old_locus_tag="MAN88_48350"
     CDS             6043..7095
                     /EC_number="2.3.1.31"
                     /GO_function="GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (48-332). E-value:
                     9.4e-31. Score: 99.2. Matches aSDomain:
                     nrpspksdomains_LC603172_RS24890_Abhydrolase_1.1"
                     /codon_start=1
                     /gene="metX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002795959.1"
                     /locus_tag="LC603172_RS24890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48350"
                     /product="homoserine O-acetyltransferase"
                     /protein_id="WP_061432704.1"
                     /sec_met_domain="PF00561 (E-value: 4.8e-42, bitscore:
                     142.7, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDRLDLISPETRFYSPPHPFVLESGAVLERVRIAYRTWGILNNKR
                     DNGVIVCHAFTGWADVEAWWEPLLGTGRVLDPTRDFIVCSNILGSCYGTTGPTSIDPRT
                     GKPYGPTFPEITIRDLVHLQRLLVDALGIESLRLVIGGSLGGMQVLEWALLYPEKTRSI
                     AVIAASGRHSAWCIALGEAQRGAIYADPHWQGGNYALDRPPARGLSAARMMAMTTYRSH
                     ASFESRFGREYGEGGEFTIARYLEYQGRKLVERFDANAYITLSRAMDRHDVTRTGKSYE
                     SVLQSIQQPTAIVAIDSDLLYPPVEQEELAKYIPNSELFWLRSPHGHDAFLMDGDMDAL
                     NDLLVTFGNG"
     aSDomain        6187..7038
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LC603172_RS24890_Abhydrolase_1.1
                     "
                     /evalue="9.40E-31"
                     /label="LC603172_RS24890_Abhydrolase_1.1"
                     /locus_tag="LC603172_RS24890"
                     /protein_end="332"
                     /protein_start="48"
                     /score="99.2"
                     /tool="antismash"
                     /translation="VIVCHAFTGWADVEAWWEPLLGTGRVLDPTRDFIVCSNILGSCYG
                     TTGPTSIDPRTGKPYGPTFPEITIRDLVHLQRLLVDALGIESLRLVIGGSLGGMQVLEW
                     ALLYPEKTRSIAVIAASGRHSAWCIALGEAQRGAIYADPHWQGGNYALDRPPARGLSAA
                     RMMAMTTYRSHASFESRFGREYGEGGEFTIARYLEYQGRKLVERFDANAYITLSRAMDR
                     HDVTRTGKSYESVLQSIQQPTAIVAIDSDLLYPPVEQEELAKYIPNSELFWLRSPHGHD
                     AFL"
     gene            7265..7675
                     /locus_tag="LC603172_RS24895"
                     /old_locus_tag="MAN88_48360"
     CDS             7265..7675
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012372.5"
                     /locus_tag="LC603172_RS24895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48360"
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_061432705.1"
                     /transl_table=11
                     /translation="MSELQLSLFPQNNQDIEKTKKVKLGRYEKIQRELLTIKRDLHQVF
                     VDVNCPIAPHLTYNFIDLFCGAGGITQGLVQANFHPAASVEINPVASLTHRHNFPQCHH
                     FCGDIHDFQAEKWGSSVCVMQWRGVIRDGTLI"
     gene            complement(8077..9000)
                     /locus_tag="LC603172_RS24900"
                     /old_locus_tag="MAN88_48370"
     CDS             complement(8077..9000)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS24900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MAN88_48370"
                     /product="hypothetical protein"
                     /protein_id="WP_061432706.1"
                     /transl_table=11
                     /translation="MMALSPFDESNKINIEAYFHWLVTRINHLPDSDINSLLQTYIAIV
                     ICHDIEPKILNESIKIVKNLTAKLILLSEHQFRSSNLILLSLAAISTYRLLGTQLPNIV
                     DHVKKVIDKLPEKSSDNSLNERPILFLAGTLNLIPKPSFKVMNYKPDLLALLNADDLAI
                     QKSITELENLTVYGIVRTPIYHGLIDVLEVILFEYLQNYNLKIALRLFRMLNYLNVSTK
                     YGIQKSFAFINNQQCPDGSFGFSELEIAKMDIEFQFNDLKNWFKLQNTFSYLWLTAEQM
                     NSDYRLLRDIGRGPIQGSSLYQFSRV"
     gene            complement(8997..9956)
                     /locus_tag="LC603172_RS24905"
                     /old_locus_tag="MAN88_48380"
     CDS             complement(8997..9956)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF046440.1"
                     /locus_tag="LC603172_RS24905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48380"
                     /product="DUF6895 family protein"
                     /protein_id="WP_061432707.1"
                     /transl_table=11
                     /translation="MLNYFDILLPSLLERTLDWITQHIDYFSLPNHKSSDREVKATIEH
                     KAFGELAIACLVMNRYPQVRQRQEFKVLIGHIVEELEKSNFTFNMTRRFGLFEFYLSIY
                     SCLKACDLKTTIEVEQLKILLQKMIDEDFIDAVERTPYSQMELSYYFKLGGFKHRLSDC
                     SSIYKYSSAFHLPSPIHLRITDAYNLTHIIFYLSDFGQQPLAPILLERYNEIYQYIILI
                     LGLYTKKKDWDLVAELLICCNFLQCDYFHPLIQLAGKSLGEIQNPQGFIPAKSRLSEKQ
                     ESNSEEVNLEKDTFRKNYHPTLVTFLASMFYLYGGNYL"
     gene            complement(10001..12019)
                     /locus_tag="LC603172_RS24910"
                     /old_locus_tag="MAN88_48390"
     CDS             complement(10001..12019)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002762755.1"
                     /locus_tag="LC603172_RS24910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48390"
                     /product="PatA/PatG family cyanobactin maturation protease"
                     /protein_id="WP_061432708.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 6.4e-23, bitscore:
                     79.8, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 1.2e-176,
                     bitscore: 586.4, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDIIDIPGITELWTRTLGSPQITVAVLDGPADLDRACFQKANIA
                     QIKPYWQEMLEVPQEYIDLYIDLENREEDSEEKAAKMESAIPAPFGDYLNFVSHATHIA
                     STIFGQHDSPVKGLAPNCRGINIPLGYDFDNFSSPLNLSRAINLALNAGANIIHIAVCI
                     PTQTGVAHDLLERAVRQCQDNNILLVAPGGNNEGECWCIPAVLPHVLTVGAMKDNGQPF
                     KFSNWGGEYQNQGVLAPGENILGAQPWSDKPLRKKGTSCAAPIVTGVAALLMSLQLQQG
                     QEPDAEAVRAAILNSAIPCDPEEVEEVERCLLGRLNVPSAINLVDKKPLLIVASSGFET
                     AWKIEAMAVTDSIAQPDPISSILEDSVMPTDSSPAITPSQTADSITPSARTNLVYALGT
                     LGYDFGTEARRDSFKQLMPRQSIPGYPAGTPPVLANPYDARQMADYLAQNPSEAKSLIW
                     TLNMELTPIYAIEPVGPFAYAVYELLQGLLQGEVQPENSSEYIDRVSIPARLTGKTVKL
                     LSGQEVPVIRPDGERGIYGWKVNSLIDAALEAVPVNEQPQLAAVLSNFLNRIYYDFSNL
                     GQTASDRALNFAATNIVQSVSVFSESILLGMELDSITVEKSPYCRLNSDCWDVALKFFD
                     PENNDRARKVYRYTLDVSDIFPVTLGGEAKTWWDSPN"
     gene            complement(12106..12978)
                     /locus_tag="LC603172_RS24915"
                     /old_locus_tag="MAN88_48400"
     CDS             complement(12106..12978)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04445.1"
                     /locus_tag="LC603172_RS24915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48400"
                     /product="LynF/TruF/PatF family peptide
                     O-prenyltransferase"
                     /protein_id="WP_061432709.1"
                     /transl_table=11
                     /translation="MINYANAQLHKSKNLMYMKAHENIFEIEALYPLELFERFMQSQTD
                     CSIDCACKIDGDELYPARFSLALYNNQYAEKQIRETIDFFHQVEGRTEVKLNYQQLQHF
                     LGADFDFSKVIRNLVGVDARRELADSRVKLYIWMNDYPEKMATAMAWCDDKKELSTLIV
                     NQEFLVGFDFYFDGRTAIELYISLSSEEFQQTQVWERLAKVVCAPALRLVNDCQAIQIG
                     VSRANDSKIMYYHTLNPNSFIDNLGNEMASRVHAYYRHQPVRSLVVCIPEQELTARSIQ
                     RLNMYYCMN"
     gene            complement(13721..13984)
                     /locus_tag="LC603172_RS24920"
                     /old_locus_tag="MAN88_48410"
     CDS             complement(13721..13984)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS24920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MAN88_48410"
                     /product="hypothetical protein"
                     /protein_id="WP_061432710.1"
                     /transl_table=11
                     /translation="MKNPTLLPKLTAPVERPAVTSSDLKQASSVDAAWLNGDNNWSTPF
                     AGVNAAWLNGDNNWSTPFAGVNAAWLNGDNNWSTPFAADGAE"
     gene            complement(14290..14469)
                     /locus_tag="LC603172_RS24925"
     CDS             complement(14290..14469)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS24925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_221275414.1"
                     /transl_table=11
                     /translation="MNQMKKKTLLPQLTESIKRPSINVPSSPMPELGGVETAIYIPGLG
                     GVLQGIPFASDDAL"
     gene            complement(14929..15339)
                     /locus_tag="LC603172_RS28615"
                     /old_locus_tag="MAN88_48420"
     CDS             complement(14929..15339)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04220.1"
                     /locus_tag="LC603172_RS28615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48420"
                     /product="cyanobactin biosynthesis system PatB/AcyB/McaB
                     family protein"
                     /protein_id="WP_081106828.1"
                     /transl_table=11
                     /translation="MIRNETPIREDIGRFLSIVFGLERTNQLNLLLDKDLVDLCPPIEP
                     YQVTKNQLKVKFKMIPIQAKPVKRPDVIAPHLAVDVVHNDGSRISDPTSINNLVVIRIR
                     LLHGANYNDPPYFQSKSYQQIKSSGLRGSLLK"
     gene            complement(15375..17348)
                     /locus_tag="LC603172_RS24935"
                     /old_locus_tag="MAN88_48430"
     CDS             complement(15375..17348)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771628.1"
                     /locus_tag="LC603172_RS24935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48430"
                     /product="PatA/PatG family cyanobactin maturation protease"
                     /protein_id="WP_061432711.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.7e-33, bitscore:
                     114.5, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 2.3e-204,
                     bitscore: 677.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDITEILGIQELWRKTRGDPRICVAVLDGLVDKSHPCFQGAHLS
                     RLPTLVDGEATSQGDMSKHGTHVSSIIFGQHGSPVEGIAPSCRGLIVPVFADRGQRLSQ
                     LDLSRAIEQAVSSGAHIINISGGQLTDFGEAEDFLQRVTQLCEKNNILIVAATGNDGCP
                     CLHVPAAIPSVLAVGAMDNLGKPMEFSNWGKNYQTQGILAPGDNILGAKPGGGVVRLSG
                     TSFATPIVSGVAALLLSLQLQQGEIPDPHKVRDAILNSALPCDPNQIKSNYHCLVGKIN
                     ISGAIELLQGGTMSELRETIAPAGCGCDVVNNIEADVANTNEIEASANTSEISHSIPET
                     ALAASVISPSSSQNSLTSNVSKMSNKGSNFITASQPAETIGENLVYALGTLGYDFGTEA
                     RRDSFKQLMPWQQIPDSIPIPPNPYDARQMVDYLAQTPSEAKSLIWTLNLDLTPIYAIE
                     PVGPFANNVYGLLQQLLSGEVQPENSDEYIERVSIPGRLTGKTVKLFSGQVVPVIQPEN
                     ERGIYGWKINSIINAALEQVPTEQRDSVVRTLQSFLSRIYYDFRNLGTTSQDRAINFAA
                     TNLIQTAQTASQAIESGFELDTITVEKSPFCRLDSDCWDIKLKFFDPENSRRARRVFRY
                     TIDVSDIFPVTLGEVKQWSAPY"
     gene            complement(17979..19601)
                     /locus_tag="LC603172_RS24940"
                     /old_locus_tag="MAN88_48440"
     CDS             complement(17979..19601)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768434.1"
                     /locus_tag="LC603172_RS24940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48440"
                     /product="metallophosphoesterase"
                     /protein_id="WP_239698171.1"
                     /transl_table=11
                     /translation="MRFIFELAQLQQSATEIKQMLDQKIFKIGVLKRAMSKNKIIVLSD
                     IHIGDNSPTVWYQKSFHEPYLTAVLDHVIKNASSIQELILLGDIFDFWTYPPDKRPPSF
                     EQIIEQNPNILGPQGKLSQVLTALEGQVTYVRGNHDMNVTQEDLNKIQNPDYTIKLSPS
                     DIYFPLGEDNKKIVCTHGHLYAMFNAPDTSVKFNPLPVGHFVSRAAAYELQQTLPPGKT
                     VADLTGQTSPNSIDLKSLATTIMAAQSGFSVTDLLLNYITQSTKMPEIQPIILPDGQTT
                     TIAEVKPLYSQLWQQWINNNGGARDGLLVAIKAALADAKDYYMGWFAQKLALECGADLV
                     VMGHTHTPISGLKKGLIQYVNSGFECPSKPDIGKQHVNFIEIDTDSYQGAIFKVVNQQG
                     SYQIEADSAEQTSVIIPGLSQDYSCYVTVENKSPILPIQLVRYEANEGHYIVAPTQFIG
                     PREKGRFWIQDYPGITKGSEGRLIYFTGDREIMLRYSCPVGLSPNSCSGAEFYTSVDGI
                     NWGERNQIVNKGIGHPFFVRFVL"
     gene            19903..20853
                     /locus_tag="LC603172_RS24945"
                     /old_locus_tag="MAN88_48450"
     CDS             19903..20853
                     /EC_number="2.1.1.37"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012264102.1"
                     /locus_tag="LC603172_RS24945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48450"
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_260324464.1"
                     /transl_table=11
                     /translation="MSIVFDLERTNQLSLLPDKDLADLCPPIEPYQVTKNQKWLILAGS
                     PDIHLVVGGPPCQGFSVAGKRDPNDPRNQLFKEFIRVVSEIQPWYVVMENVPGILTMQK
                     GKVKETILEEFESIGYSNISIAILESAAYGIPQIRPRAIFIANRFDLPNPYPQPQLLPH
                     QYIPIESAISDLPAYTPIPEINHEWTRHSSKYMERIARVPPGGSLYETYVDAFKRQYPG
                     KPSMTIKENHGGTHIHPHLNRVISAREMARLQFFPDSFIFEGTMKKAMWQIGNAVPPRL
                     AECIGYALIPYLNKVALQLEQESQINQNLNPIACG"
     gene            21052..22239
                     /locus_tag="LC603172_RS24950"
                     /old_locus_tag="MAN88_48460"
     CDS             21052..22239
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1192: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517330.1"
                     /locus_tag="LC603172_RS24950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48460"
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="WP_061432712.1"
                     /transl_table=11
                     /translation="MEKAYSFRFYPTPEQESLLRRTLGCVRLVYNKALHERTQAWYEKQ
                     ERVGYAETSSMLTDWKKQEELDFLNEVSCVPLQQGLRHLQTAFTNFFAGRNKYPNFKKK
                     HQGGSAEFTKSAFKFKDRQIYLAKCTEPLPIRWSRQIPESCEPSTVTVRLHPSGRWHIS
                     IRFDDPTIQPLPPTDKAIGIDLGISSLVITSDGDKVSNPKHFKKHYRRLRKAQKSLSRK
                     QKDSKNREKAKIKVARIHAQITDSRKDHLHKLTTQLVRENQTIVVENLAVKNMVKNPKL
                     SQAISDVSWGEITRQLAYKCRWYGRNYIEIDRWFPSSKRCSNCGYIAEKMPLNVREWDC
                     PDCGTHHDRDVNASKNILAAGLAVSVCRATIRPEQSKSVKAGAEPRKGKKQKPKS"
     gene            complement(22229..23044)
                     /locus_tag="LC603172_RS24955"
                     /old_locus_tag="MAN88_48470"
     CDS             complement(22229..23044)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS24955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MAN88_48470"
                     /product="hypothetical protein"
                     /protein_id="WP_061432713.1"
                     /transl_table=11
                     /translation="MEKIVQHGQRRHSKASESYIDVTFRYDDGTIWEGAIPVEYRRTGV
                     DLAESSAIEEYLQQAFLYCHPSNYPKWRQEQEVFWLQKEAEVTKSFFDVLITFKWTCVA
                     CQLPPNPNWARRIQDLKEMGYTIATHTSKKCPTCGSKKTHIILVPLPRGGISGYEVWSS
                     SLRKKIIDLLGGYDAYEGKTVGKDNLLPDHKFPEIRWGNDTRRDSLEHLADTEIREQFQ
                     LLTNQRNLQKREVCRKCYQTGDRGYPFGIQYYFDILTAVNGRRFPNLTI"
     gene            complement(23013..23288)
                     /locus_tag="LC603172_RS24960"
                     /old_locus_tag="MAN88_48480"
     CDS             complement(23013..23288)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002759092.1"
                     /locus_tag="LC603172_RS24960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48480"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_052278399.1"
                     /transl_table=11
                     /translation="MDENKAKILKALGSLVRQHRENLGISQEELATRSNLDRTYISGIE
                     RGIRNPSVTALVSLANGMNLNVSNLLEGLENKVNQTNGKDRTTRTT"
     gene            complement(23391..24098)
                     /locus_tag="LC603172_RS28620"
     CDS             complement(23391..24098)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1170: metallo-beta-lactamase family protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002756061.1"
                     /locus_tag="LC603172_RS28620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_239698179.1"
                     /transl_table=11
                     /translation="MFRQLFDRESSTYTYLIADSGTGEAILIDPVLEQVDRDRQILWQL
                     GLTLGYTMETHVHADHITGAHRLRELTNCSILVPENAEVSDIDGYVRDGDLWTVAGQQL
                     KAIATPGHTDSHIAYLIDEKRLLTGDALLIRGCGRTDFQNGSPEVLYKTVTEKLFTLPD
                     DTLVYPCHDYLGRTVSSIGEEKHWNPRFAGRNREDFIQLMNNLNLPYPKKMTAALSANA
                     RGGKVVFVMDYQI"
     gene            complement(<25062..25541)
                     /locus_tag="LC603172_RS28625"
                     /pseudo=""
     CDS             complement(<25062..25541)
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024846.5"
                     /locus_tag="LC603172_RS28625"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="FAD/NAD(P)-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSYIDRNQFSATFDIAIIGGGFSGSLVTANLLRDTGTPLSIALIE
                     RRKPLGTGIAYGTRDSGHLLNIPAGKMSAFEDDPEHFLHWLADNGYRSIEPASFVPRLV
                     YGKYIRSILEEARDNAIADHRLETFTDAAIDLALDGEKATITLKGGKKISAAKVVL"
     misc_feature    25505..25519
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.37"
                     /tool="antismash"
     misc_feature    complement(25518..25531)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 20.35"
                     /tool="antismash"
     gene            complement(25805..26443)
                     /locus_tag="LC603172_RS24970"
                     /old_locus_tag="MAN88_48500"
     CDS             complement(25805..26443)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207371.1"
                     /locus_tag="LC603172_RS24970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48500"
                     /product="cadmium resistance transporter"
                     /protein_id="WP_061432715.1"
                     /transl_table=11
                     /translation="MNWLVALLITSVTSFVATNLDDLMVLMLFFSRLNANFRPRHLIIG
                     QYLGFTLILLASSLGLLFGLLVSKEWIGLLGFIPLIIGIKQLLSRENEDYIQEVREDVN
                     YSKNRSFIPRFPSQTYYVAAVTVANGGDNIGIYTSLFANNSPMHVLIIMIVFYLMMGIW
                     CALAYFLITHPRIAKVVTNYGHKISPFIYIALGIYILIDSQFYRLFLTY"
     misc_feature    complement(26458..26473)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.38"
                     /tool="antismash"
     gene            complement(26474..27049)
                     /locus_tag="LC603172_RS24975"
                     /old_locus_tag="MAN88_48510"
     CDS             complement(26474..27049)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731893.1"
                     /locus_tag="LC603172_RS24975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_48510"
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_024968956.1"
                     /transl_table=11
                     /translation="MTLTYQPSTPHRWHFDNRAILPLRNPNFWKIESGVVKTSTWLEDG
                     TLIVLGLWGPGNFVGKTLERVNPYQVECITPVQAVSFSTVESYQMAEILLSHLQQAQEL
                     SIIRSYKRTDVMVVKLLSWLGNQFGKQTGTGQLIDVRLTHQDIADLLGTTRVTITRALN
                     HLEQQGAVERLPVHRLLLREEEIWHYEI"
     misc_feature    27127..27142
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 22.93"
                     /tool="antismash"
     misc_feature    complement(27133..27146)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.45"
                     /tool="antismash"
     misc_feature    complement(27243..27257)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.19"
                     /tool="antismash"
ORIGIN
        1 cggcccaact atccgggggt caacgtcaac gggttgccct agcccgggcc ttagcggtgg
       61 aaccggaagt attattatta gatgagcctt ttggggcctt ggatgccaaa gttagaaaag
      121 agctacgagt ttggttgcga cagttacacg aggaggttca tgtcaccagt gtttttgtca
      181 cccacgacca ggaggaagcc atggaagtgg ccgacgagat cgtaattttc aatgagggta
      241 aaatcgaaca ggtgggaact cccgatcagg tgtacgatca tcctgcctct gcttttgtga
      301 tgagttttat cggccgggtg aatgttttac caccgacctc ggctgcacta ttcgagcagc
      361 tcggaggggg tactaacgga gtcaagattt tcattcgtcc ccacgaccta gaaattctct
      421 cttctcccaa tgggtctagc attgaggcaa aagttgagcg cattatccat ctgggttggg
      481 ccatcgatgt ggaattaatt ctacccgatc gctctctcct gattgcccat ctgaatcgag
      541 aacaactgga tcaactgcat ctccaagtcg gggagcaagt ttatgttcgt cctcgggatg
      601 ctcgcgtttt tgcttaaaag taaggacgtt gctgatttga gatctgaatc ttcttttgtg
      661 ggatttttca aacctagacc caaactaact tttgcacggg aacctgcacg gttggcattc
      721 ataccttatt cagcaacgcc aactttttta gaatagattg ggatcgacac cgatcgcgcc
      781 ttcaccgtcc gcatcttgca ggttagcacc gtctaaccga gcattagtga gattggcctt
      841 ctctaagtca gctttttgca gattggcgtt ggttaagttt gctccttgca ggttagcttt
      901 ttctaaatct gcacctaaga gattggcctt gcttaaatcg gctcccgcta ggagagtttt
      961 gccgaggtcg gctccccgga ggtcagtccc tttgagatta gcaccctgta ggttagttct
     1021 ttctagatcc gcttgactga ggtttgctcc ttctagattg gccgaggaca agtttaaatc
     1081 tttgaggcta attccgttaa agtcacaacc gacacaggcg ttagtttcct gtagtttttg
     1141 cagattatcg gcggcagctg gctcgaccag agtaattttt gccggtgcgc cgccagtggg
     1201 ggtaacggcc ccagcgatgg cagggtggaa gcaagaaaaa actgtgatta gcaatagaca
     1261 gaaggctgtc agtagcttcg gtttcatcgt ttttgatctc cttgagtgat ctttttgagt
     1321 ctctgttttc actctaggca atggctttaa taaaaacaaa tattctttgt tttcaaatcc
     1381 ataaattccc tttatgcaaa gctgggaagc cccctcgccg ttccatcact tttatttatt
     1441 aaaacgacaa ttaagaatat ttgcttttat gcttgtcccg tacctaagat gatagtgtcc
     1501 ggccaaaatc ctagtcaagg agagcgtttt cagcaatctc gccatcgatc gcaagctcaa
     1561 gaacttgacc ttagatttat accgacaaaa aaatagtcaa gaatcctagc aggaaatacc
     1621 aagacgcatc tacaacgtga taggagtaaa aatataatta tatgtcccct caaattctcg
     1681 atatagctga aaatctctct ctaaccactt gcggcactat tctcaccctg gcagtcttag
     1741 aaaccattct atcggccgat aatgccgtcg ccctggccgc tttggtgcga gaacttcccg
     1801 accccagaca acaacgccag gctttaaact ggggattggc cggggcattt gtgttaagag
     1861 ttgccctgtt agtcagtgcc agttggacag ttaaattctg gcaggtggag gtactgggtg
     1921 ctatttatct gatctggtta gcgggtaagc atttttggca gaaatttttg aatatagaaa
     1981 tagaagacga aaattttgcc actaatctcg ccatagaatc ttttggccgt gttattgttt
     2041 tgattgcttt tacagatctg gctttttccc tcgatagtgt gacaacagcg atcgccctag
     2101 ccgatcgatt ctggattctc ctgactggtg gtattttggg aatgttagcc ctacgatttt
     2161 tgaccggttt atttgtcagg tggttagctg agtttgttta cctacaagat gcggcctatc
     2221 tgacggtttt agcggtaggg ggaagattgt tattaaaagc ttgtcaacca acctatttgc
     2281 cgccagagtg gatggtattg atgatggtgg cggcctcctt aagttggggc ttttctaggc
     2341 gtgttgtggt ggaatagttc tggcagcaag ggattttctc aattttttag gagcgtgtca
     2401 atcgttatac tccgggagga tgggcgactg tgacataatt tgggactagg tacggtaaga
     2461 gtcgctagtg aaccccgtta gccttggaga cttccttgct agacaccatt tccaccctat
     2521 ccccaaaact aacagcgatc gaaaccaagg gaaatcctct ccccaaagtt gtcagttgtt
     2581 cctccacctg tcgttattgt cggttttata agtttcaggg gagaggaaac ggctattgtc
     2641 aacgcttggg agtcgctgtc cacagtcatt ggcgcgcctg tcctttgatt tgtccacctt
     2701 tcgccccttc ctgggaaagg tcggaaaatg ggtaattcta agtagttatc tggattttac
     2761 cgcctgtatc gagcaatttt ctccccatcc cgctccgtcc gaggatgggg ttttaatcga
     2821 aaccgatgct ttctactcca ccgctatcgg gactggtggc gacaaaatag agaattcccg
     2881 accctagaca agcgatcgct atactaaagc aaataatcca gacttgagca ggttcgtagc
     2941 tattactatc gatcgctcgc cggatgagaa aatatcggcg cgtggcgatg acaacggtta
     3001 ataatcccac catagcgaag acaaaaccca acaaccaacc cagtcctaga gatgacgaag
     3061 atactgttcg taaacgcaaa atgaccagac caaaacccat caaagcgatc gaggcacgca
     3121 tccaagacag ataggtgcgt tcgttggccg aatgatctcg ccagtaatcg gggttaaatt
     3181 tctgctttgt cttttgggat ttaaacagca tagaggagtt cctcgcagtg aacttcaata
     3241 aaatcaagta ttctttctta tggttttaat aaattgtttt tatcatttag tttttcgctt
     3301 atctttagcc aattcaaggg acttaagcag attatcatcc ataaataggg gaagtttacc
     3361 gtcccatctt tcgatcgctt tgttctgtaa aacttctgga cttaaggaat cttggagaat
     3421 gcgatgagct gccgcctctc ccttagccag attgattttt acttccgatt ctttcaaggc
     3481 tttcagcacc ataaaaccgg cttttttggc ctcctgttcg gcaatttgtt tggcttccac
     3541 cgcatcggta aagcgatcgg aaaaattgac atgaaccaaa gaaatatcat cgacaccgat
     3601 atgataattt ttcattcttt cggtcaaacg aatatccact tctcctttca cctcctcccg
     3661 tttagtaatt aattcttcgg cggtatattt agccataact gccttgagaa tttcctcaac
     3721 tgctggatcg atcactcgtt caatgacagc cgcttcatcg ccaatttgtt gaaaaatagt
     3781 attaacttct gatgctaaaa tatgccaatt catggccaca tctgtgaata cttcctgtag
     3841 gtctttggag gaagcttcgg ccgctatctg ttgtttttga attctcacac ttagtttttt
     3901 caccgtgtta acaacgggaa taatcccgtg aataccctca ttaagtattt tatcctgtac
     3961 ttgaccaaaa accattaaaa cccctctttc tcccgcatta acaatcacga aaggattaaa
     4021 aataatcgcc aaaattaata acaaaaaaaa gagattactt ggtctagcta aaagacgaat
     4081 tttgagcatt ttaattgata atatagatgg ttgataggat agttttgcaa ataaataaaa
     4141 tgactaattt tatgttggta attagcgata tttttatcta ccttaagaat tttatcaact
     4201 agaaaatgaa ggtaaagttg tcctatatta tttagatgaa gtcagattta gcctcgttat
     4261 tttttccttc tggctggcaa aatatcggga aataattgtc tatttatagt taccatagtc
     4321 gccgattaaa tgttttacta atcatgaatc aaaacgatta tctcgttagt tttagtcaga
     4381 aacttgacca aaaaagcatt tatttgtcca agaaaggaat cgggattatc cagaaacaat
     4441 tataacaaaa tttcccttgt cttaaatggt acattctcat tccaccagtc tagctggtac
     4501 tttcttttat agcaattacc ttaacacaat ctagtttcgg cataaaagat attctgtata
     4561 caggatatct cgataaattg actcgcagaa atggctatga tagaaaaata cagtaagcga
     4621 ataagcctac tgtacccatt ccatccccta atgaggtcat ttaatcaccc tttttcctcg
     4681 agcgcacaga tcgaggaatt tatcctttat tttcgtccct gagcgcccat gtctgaccgt
     4741 taccgtttcg agacgctaca agtacacgcc ggccaggaac ctgcccccgg aaccaacgcc
     4801 cgcgccgttc ccatctatca aaccacttcc tacgttttca acgatgccga ccacggggcg
     4861 cgactgtttg ccctggaaga gttcggcaac atctacacca ggatcatgaa cccgaccacc
     4921 gatgtcttcg agaagcggat cgccgcttta gaaggaggcg cggcggccct ggccacctct
     4981 agcggtcagg cggcgcaatt tctagcgatc gctaatattg ccggtgcggg ggataatatc
     5041 gtttctacta gctacctcta cggcggaacc tataatcagt tcaaagtctc cttaccacgt
     5101 ttaggaataa atgtcaagtt cgtcgatggc gatgaggccg aggcttttcg agcggcgatc
     5161 gacgatcgca ctaaagcctt atacgtcgag acgatcggca atccccgatt taatattccc
     5221 gattttgccg ccctggccca tatcgcccac gagaacggca ttcccctcat cgtcgataat
     5281 accttcggct gcggcggcta tctctgtcgc ccgatcgaac agggggccga tatcgtggtg
     5341 caatcggcca ccaaatggat cggcggtcac ggcacttcca tcggcggagt aatcgtcgat
     5401 tcgggtaaat tcgactgggg aaacggcaaa tttccccagt ttaccgaacc cgcaccgggt
     5461 tatcacgggt tgaatttcta tgaggtgttc ggaccgtccg gccctttggg caatatcgcc
     5521 tttattatcc gggcgcgggt ggagggattg cgggattttg ggcccgcctt gagtccgttt
     5581 aacgcatttt tactgctaca gggattggaa accctctccc tacgggtcga tcgtcatgta
     5641 tccaatgcgt tggccttggc gaactggctc aaagaacagc cacaggtgga atgggtggat
     5701 tatcccggat taccggaaca tccttaccac gaacgggcga agaagtacct aaaacacggc
     5761 ttcggcgggg ttttagcctt cgggattcgg ggcggtttcg aggccgggcg gaattttatc
     5821 aaccgggtga aactagctag tcatctcgcc aacgtggggg acgcaaaaac cctggttatc
     5881 catcccgcct cgacgactca ccagcagtta aacgaagaag aacagcgatc ggcgggagta
     5941 acgcccgaat taatccgcgt ttccgtgggt ttggaacata ttgacgatat tatcgaggat
     6001 ttccagcaaa ctttccagag tttataaccc accaccccgc ccatggatcg cctagacttg
     6061 atctccccgg aaacccgttt ctattcccca ccgcatcctt ttgttctcga atcgggggcg
     6121 gttctcgaaa gggtgcggat cgcctatcga acctggggaa ttttgaataa caagcgggat
     6181 aacggggtta ttgtctgtca tgccttcacg ggatgggccg acgtggaagc ttggtgggaa
     6241 cctttgttag gaaccggacg cgttctcgat ccgacccggg attttattgt ctgtagcaat
     6301 attctcggca gttgctacgg gacaaccggc cccacttcga tcgatccccg taccggtaaa
     6361 ccctacggtc cgactttccc ggagatcacc attcgggatc tggttcacct acaacggctg
     6421 ttggtggacg cattggggat cgagtctttg cgattggtga tcgggggttc cttgggagga
     6481 atgcaggttt tggagtgggc gctactgtac ccggagaaga cgcgctcgat cgctgtcatc
     6541 gccgcttcgg gacgacattc ggcttggtgt atcgcgctgg gagaggctca aaggggagcg
     6601 atctatgccg atccccattg gcaaggggga aattatgcgc tcgatcgccc tccagcccgg
     6661 ggtttatcgg cagcccggat gatggcgatg actacctacc gatcgcacgc cagtttcgag
     6721 tcccgtttcg gccgcgagta cggggagggg ggagagttta cgatcgcccg ctatttagaa
     6781 tatcagggga gaaagttagt cgaacgtttt gacgccaatg cttatatcac cctaagtagg
     6841 gcgatggatc gtcatgatgt cacccggacg ggaaaaagtt acgaatcggt gttacagtcg
     6901 attcaacaac cgacggcgat cgtggcgatc gattcggatc ttctttatcc ccccgtagaa
     6961 caagaggaat tggcgaaata tatccccaat tctgaattat tttggttgcg atcgccgcac
     7021 ggtcacgatg cgtttttaat ggatggcgat atggatgcgt tgaatgatct tctcgtcact
     7081 ttcgggaatg gatgagcaac gggaaatagt cgcgggttgg ggatggtcgg ggtagagagc
     7141 gatcggggat tgatgacgac caggttaaaa gtagcttgac tttgttactc taccttccat
     7201 aggcgtgact tgattttttg tggacgatgt gtcacaataa accatatata ttcaagtcag
     7261 agcaatgtca gaacttcaat tatcattatt ccctcaaaat aatcaagata ttgaaaagac
     7321 gaaaaaagtc aaattaggac gttatgaaaa aattcaacgg gaactactga ccatcaagcg
     7381 tgatttacat caagtttttg tggatgttaa ttgtccgatc gctcctcatc taacttataa
     7441 cttcattgat ttattttgtg gtgctggtgg cataactcaa ggtttagttc aggcaaattt
     7501 tcatccagca gcaagtgttg aaatcaatcc tgtcgcttca ttaacacatc ggcacaactt
     7561 cccacaatgc catcatttct gtggtgatat tcatgacttt caagcagaga aatggggctc
     7621 ttcggtttgt gttatgcaat ggaggggagt tataagggat ggaaccctta tatagaaagg
     7681 catttagcta tttttgtcaa ttgtttttta tctagagcga actaatcaat taagtctctt
     7741 gccagataag gatttagtcg atttatgccc ccctatcgaa ccataacaag taacgaagag
     7801 ccgatcttat taagttcagt aatatttcta ctgtccgacc cattgattcg tctgcaatcc
     7861 cttttggctc gatggtttgc agtatctctt ctcctgattt cttgctcgaa tctagtcttt
     7921 gcgtcatgtg tcatattcta ttatattgcg tgtcccttgc ttaccttttt tctctactac
     7981 ccctacttat tgagcggata cgatcgcttt tacttttgag attctgatga gagaatgaaa
     8041 cagccctact ttttaagggg gattaaactt ctgtagtcag actctggaga attggtataa
     8101 cgaagaacct tgaattggtc ctcttccaat atctcgtaaa agcctataat cactattcat
     8161 ttgctcggca gttagccata aataggaaaa agtattttgt agtttgaacc aatttttcaa
     8221 atcattaaat tgaaattcaa tgtccatctt tgctatctcc aattcagaaa aaccaaaaga
     8281 gccatcagga cattgttgat tgttaataaa agcaaaagac ttttgaatgc catacttcgt
     8341 agagacattc aaataattaa gcatacgaaa taatcttaga gctattttaa gattgtaatt
     8401 ttgtaagtat tcaaataaaa ttacttctaa gacatcaatt aatccatgat atattggagt
     8461 tcttactata ccataaacag ttaaattttc taattcagta atactcttct gaattgctaa
     8521 atcatctgcg tttaataaag ctaataaatc tggcttgtaa ttcatcacct taaatgaagg
     8581 ttttggtatt aagtttaaag tgccagcgag aaaaagaata ggcctttcgt tcagcgagtt
     8641 atcacttgat ttctctggca atttatcaat gacttttttt acatggtcaa caatgtttgg
     8701 aagctgagtt cccaataatc tgtaggtaga aatagcagca agcgataaaa gtatgagatt
     8761 actgctgcga aattgatgtt ctgataaaag gattaatttt gctgttagat ttttaactat
     8821 cttaattgac tcattgagaa tttttggctc aatatcgtga caaattacta tcgctatata
     8881 ggtttgtaaa agcgaattta tatcagagtc aggcagatga ttaatacgag taacaagcca
     8941 atgaaagtaa gcttctatat taattttatt actttcatca aaaggagata atgccatcat
     9001 aaataattcc ctccatagag atagaacata gaggctaaaa atgttactaa agtaggatga
     9061 taattttttc taaaagtatc tttttcaaga tttacttctt ccgaattgct ttcttgtttt
     9121 tcggacaatc tactttttgc tggaataaaa ccttgaggat tttgtatctc accaaggctt
     9181 ttccctgcta attggattaa aggatgaaag tagtcacatt gcaaaaaatt acaacatatt
     9241 aacagctcgg ccactagatc ccaatctttt ttcttcgtat ataatcccag tataagtatt
     9301 atgtattggt agatttcatt gtatcgctct agtaatattg gggctaatgg ttgctgtcca
     9361 aaatcgctta agtaaaagat aatgtgtgtc aagttataag catcagtaat acgcaaatgg
     9421 attggagatg gaaggtgaaa agcactggag tatttgtaga tgctactaca atcagaaagg
     9481 cgatgcttaa agccaccgag cttgaaataa tagctgagtt ccatttggct gtaaggagtt
     9541 ctctctacgg catcgatgaa atcttcatca atcatctttt gtagaaggat ttttaattgt
     9601 tctacctcta tagttgtttt tagatcgcaa gcttttaggc atgagtatat agataaatag
     9661 aattcaaaca ggccaaacct acgcgtcatg ttaaaagtaa agttactttt ctctagttct
     9721 tctacaatat gtccaattaa aactttaaat tcctggcgtt gtcgaacttg tgggtagcga
     9781 ttcatcacca gacaagctat ggccagctcg ccaaaagctt tatgttcaat agtagctttg
     9841 acttccctat cgctcgactt atggttgggc aggctaaagt agtcaatatg ttgagtaatc
     9901 cagtctagtg tcctttctag gagagaggga agtagtatat cgaagtaatt aagcattctt
     9961 acccgcctta tcttgagcta tcccgcctgc aattttctaa ttagttggga gaatcccacc
    10021 aagttttcgc ttcgcccccc aaagtaacag ggaagatgtc agatacatca agggtatagc
    10081 ggtacacttt ccttgcccta tcattatttt ctggatcaaa gaactttaat gctacatccc
    10141 aacaatcact attgagccgg cagtagggac ttttctctac tgtaatgctg tccagttcca
    10201 tccccaggag aatcgattct gaaaagaccg agaccgattg aacaatattg gtagcggcaa
    10261 agttgagggc gcgatcactg gcagtctgtc ccaaattgct gaagtcgtaa tagatacggt
    10321 taaggaagtt agagaggact gccgctagtt gtggttgttc gtttactggt actgcttcta
    10381 gagcggcatc gatcaagctg ttaactttcc agccataaat tccccgttct ccatcaggtc
    10441 gaattacggg aacttcctgt cccgataaca acttaaccgt cttacccgtt aatcgggcgg
    10501 ggatgctcac ccgatcaata tattcactgc tgttttccgg ctgcacctca ccctgtaaaa
    10561 gtccttgcaa aagctcatac acagcatagg caaaaggtcc gaccggttca atggcataaa
    10621 tgggggttaa ctccatgttt aacgtccaaa ttaaagattt agcctcagat ggattttgag
    10681 ccaaataatc cgccatctga cgggcatcat aagggttagc gagaacgggc ggggttcctg
    10741 ctggatagcc agggattgat tgtcggggca ttagttgttt gaaggagtct cgccgcgctt
    10801 cagtaccaaa gtcatatccc aaagttccta aagcataaac caagttggtt ctagcactag
    10861 gagtgatact atcagcagtc tggctagggg taatggcagg tgagctatcg gtgggcatga
    10921 cagaatcttc taaaattgat gagatcgggt ctggttgagc aattgagtct gtgactgcca
    10981 ttgcttcgat tttccaggcg gtctcaaaac cactggatgc cacgatgagc aaaggctttt
    11041 tgtccactaa attgattgca ctgggaacgt ttaatcggcc tagcaaacag cgttcaactt
    11101 cttcaacctc ttccggatcg caagggatag cactgtttaa aatagcagcg cggacagctt
    11161 cggcatcagg ctcttgacct tgttgtagct gtaggctcat gagcaaagcg gcaactccgg
    11221 tcacaatggg agcagcacaa ctggtcccct tcttgcggag cggtttatct gaccatggct
    11281 gcgctcctag tatattttcc cctggggcta gcaccccttg gttttgatat tcccctcccc
    11341 agttactaaa cttgaagggc tgtccgttgt ctttcatcgc tccaactgtg agaacgtggg
    11401 gcagtactgc tggaatacac caacattccc cttcattgtt gcctccgggg gcaacgagca
    11461 ggatgttgtt atcttggcac tgacgcacag ctcgttctag caggtcatgg gctacgccag
    11521 tttgggtggg aatgcagaca gcaatatgga tgatatttgc ccccgcattg agggctaggt
    11581 tgatagcgcg agagaggttg agcggagagc taaagttgtc gaagtcgtag ccaaggggga
    11641 tgttgatccc gcgacagtta ggtgccagtc ccttaaccgg tgaatcatgc tggccaaaga
    11701 tggtactggc aatatgggta gcgtgggaga caaaattgag atagtcgcca aagggagcag
    11761 gtatggcaga ttccattttg gctgcttttt cctcactatc ttcttctctg ttttctaaat
    11821 ctatatagag gtcaatatat tcttggggta cttccagcat ttcttgccaa taaggtttaa
    11881 tttgagcgat attggctttc tgaaaacagg cgcgatcaag gtcggcggga ccgtcaagta
    11941 cggcaacggt aatttgagga ctgcctagag tgcgagtcca tagttcggta attccaggta
    12001 tatcaattat atccggcata gtttttcaca aatcttcaaa aaagttgagc aatatatcaa
    12061 aattctgttc ataagttaga gaattggctt ggcttatcca gataatcagt tcatacagta
    12121 atacatattt aatctttgaa ttgacctagc tgttaattcc tgttcgggga tgcacactac
    12181 tagacttctc accggttgat gccggtaata agcatgaacc ctgcttgcca tttcattgcc
    12241 caagttatca ataaagctgt tagggttgag ggtatgataa tacataattt tgctatcatt
    12301 agccctgctc acaccaatct ggatagcttg acaatcattg actagacgca aagcaggtgc
    12361 acagacaact ttggctaacc tttcccaaac ttgtgtttgc tgaaattcct ctgatgataa
    12421 agaaatataa agctcgatag cagtacgacc atcaaaataa aaatcaaaac ctaccaaaaa
    12481 ttcttgattg acgatcaatg ttgacagctc tttcttgtcg tcgcaccaag ccatagctgt
    12541 tgccatcttt tcggggtagt cattcatcca aatatagagc ttcacgcgcg aatcagctaa
    12601 ttccctgcgc gcatctactc ccaccaaatt tctgattact ttactaaagt caaagtcagc
    12661 tcctaaaaaa tgctggagtt gctgatagtt gagcttcacc tcagtccgac cttccacttg
    12721 atgaaagaaa tcaatcgttt ccctaatttg cttttcagcg tattggttat tatacaatgc
    12781 caagctgaaa cgagcaggat aaagctcatc cccatcaatt ttacaagcac agtcaatcga
    12841 acaatcagtt tgtgactgca taaatctctc gaaaagttct aaaggataaa gagcttcgat
    12901 ttcaaagata ttttcatgcg ccttcatata cattagattt ttgctcttat gcaactgggc
    12961 attagcgtag ttaatcattg ctttttaagt tcttgctccg aatagtcttg actatttact
    13021 tgacagaaaa ctaccttgac ctctatgaca aacctacttg acatgctcac cgccgtaaac
    13081 cgagggtgat ttctcaccac gctacctttt tagagtagtc gccgaatggt tgacgcttca
    13141 ccaaaaaacg cttaattgct taagtctgac actttctcca cgtcaattta aagtctccga
    13201 atgccctccg gcgactaatt taatttgggt ttatacatac cgacacaaga tatgtttcgc
    13261 ctctagcctg gagttcccag cggccgtcag cttcccctta ctcatcgtca acgtctcgac
    13321 gctgatcgac gttaacccct cgacgtagct cagggtttac cctgagcgaa gctgaagggt
    13381 caacccgttg ccagaatttc aacctaatat agcgtttttc agtctgctga ggtacaaaag
    13441 ttatgggttt taggcaaaag gcaagaggca aaagggaaga aaaataggtg tacctcacta
    13501 gcttaggaaa cgctatgtat ctatttcgga gtatctatga tagatagggc aggtttttta
    13561 gaccagtgag actttaacag aaagcccccc taaaagaggc ggggttttta acccaaattt
    13621 tcgataagag aggtgaaatg aaaaatgggt gagtcaataa acttatcttc atcacttcta
    13681 ctctcatctc ctctcttatt cttctgtttg ctagtgagtt ttactcagct ccatcggcgg
    13741 caaagggagt tgaccagttg ttatcgccat tcagccaagc ggcattgacc ccagcaaagg
    13801 gagttgacca gttgttatcg ccattcagcc aagcggcatt gaccccagca aagggagttg
    13861 accagttgtt atcgccattc agccaagcgg catccacaga ggaagcctgt tttaaatcgg
    13921 aagaggtcac cgcggggcgt tcaactggag ctgttaattt aggaagcaaa gtcggatttt
    13981 tcattgtttt ttttcctagt tcttggtagg ttacgaactg aagattagcc taagcttttc
    14041 tagctgaaat atacatcaca aatgctagat atgtcaactt actttatatt acttaaaacc
    14101 aatcaacagc actgcaaaaa ataagtttct gtactttact tcacagtaaa ctaaactgat
    14161 ttattcagct tagaaataga aagtatttct cgaaatcgct ctactttcac cacgggagtg
    14221 tactcaattt tatgagtcct taaacccctc ttttggttaa gatttgagac ttaaattact
    14281 gaattaagat tatagggcat catctgaggc aaaagggatg ccctgcagca cacctcctaa
    14341 tccaggaata tatatcgctg tctcaacccc acccagttcc ggcattggcg aagaagggac
    14401 atttatagat gggcgtttaa ttgactcagt tagttgaggc aataaagttt tctttttcat
    14461 ttgattcatt ttccagagta aaaaagtcaa ttccaaatgc gtcctcgacg ccactcctct
    14521 gtttctatcg tttttcgccc tagtcgggtt tgatcaccca attcttgttt gagcaggaat
    14581 ttgacatcct ttgaaccaac agaggcaagg ggtggatttg cctttgactc taagttatca
    14641 cggttcttcg ttacttggta tggttcgata gggggtcata aatcgactaa atccttatct
    14701 ggtaagagac ttaattgatt agttcgctct agatcaaaaa taattgacaa aaatcgctaa
    14761 atgcctttct atataagggt tccatccctt ataacccccg tccattgcat aacaattacc
    14821 gaagagccgt tatcactcat aaaagggtcc ttccagtttt tctaatcggt tgacaaattg
    14881 acccgtgaca ttagctttat cgcttcaaga agacgattgc tgtccgtctt acttcagcaa
    14941 agagccacgc aacccagaag acttgatttg ctggtagctt tttgactgaa agtaaggagg
    15001 atcgttatag ttagcgccgt gaagcaaacg tatcctgata acaaccagat tattaatgga
    15061 agttggatcg ctgatacggg aaccatcatt atgaactaca tcgaccgcca gatgtggggc
    15121 tataacatct ggtcttttta ccggttttgc ttgtatgggg atcatcttga acttaacctt
    15181 caactgattc ttcgttactt ggtatggttc gattgggggg cataaatcga ctaaatcctt
    15241 atctagcaat agatttaatt gattagttcg ctctagcccg aagacaattg acaaaaatcg
    15301 cccaatgtct tcccgtatag gagtttcatt tcttatcatc ctgtacgtcg cacagggcag
    15361 accgaagagc cattttagta aggtgcagac cattgtttga cttcacccaa agtaaccggg
    15421 aagatgtcgc ttacatcaat tgtgtatcgg aacacccttc tggctcggcg gctattttct
    15481 gggtcaaaaa acttcagttt gatatcccaa caatcactat ctagtcggca gaaggggctt
    15541 ttctctacgg taatggtgtc cagttcaaac ccgctctcta tcgcctgaga tgcagtctga
    15601 gcagtttgaa ttagattagt agctgcaaaa ttgatggctc ggtcttggga ggttgttcct
    15661 aaatttctga agtcataata aatccgtgag aggaaacttt ggagagttct cactacagaa
    15721 tctctttgct ccgtaggtac ttgttcgaga gccgcgttga tgatagagtt tattttccag
    15781 ccataaattc cccgctcatt ctcaggttgg atcacaggaa caacctgtcc cgaaaacaac
    15841 ttaaccgtct tgcccgtcaa tcggccagga atactcaccc gttcaatata ctcatcgctg
    15901 ttttctggct gtacctcccc gctcaagagt tgctgtaaaa gcccataaac attgttagca
    15961 aaaggaccaa ccggttcgat ggcataaatg ggggttaagt ccaagtttaa tgtccaaatt
    16021 aaggacttgg cctcggaggg agtttgagcc aaataatcca ccatctggcg ggcatcataa
    16081 gggttgggag gaatgggaat ggaatcgggg atttgctgcc aaggcatgag ttgcttgaag
    16141 gagtcccgcc gcgcttcggt gccaaaatca tatcccaaag ttcctagtgc gtaaactaag
    16201 ttctctccga tggtttcagc cggttggcta gcggtaataa aattggaacc cttgtttgac
    16261 attttagata cgttagatgt taaagaattc tgtgagctag atggacttat gactgaggct
    16321 gctaaagctg tctcagggat ggaatgactt atttctgaag tattagcact agcttcaatc
    16381 tcgttagtat tagcaacatc agcttcaatg ttgttgacta catcacaacc acagcctgcg
    16441 ggtgcgatgg tttctcttag ctcagacatt gttcctcctt gtagtaattc aattgcacca
    16501 gatatattga ttttcccaac caagcaatga taattgcttt ttatttgatt gggatcgcag
    16561 ggcaaggcac tgtttaaaat agcatctcga accttgtgag gatcgggaat ttctccttgc
    16621 tgaagttgta ggctcaacag aagggcagcg actccagaaa caataggggt agcaaaactg
    16681 gtcccgctta acctgacaac tcctcctcct ggcttggcac ctaaaatatt gtccccgggg
    16741 gcaagaatgc cttgtgtttg atagtttttt ccccagttgc tgaactccat tggcttgcct
    16801 aggttatcca ttgcccccac tgctagaaca gaaggaatgg ctgctggaac gtgcaagcaa
    16861 ggacatccat cgttacctgt tgctgcaacg attaaaatat tgtttttttc acacaactga
    16921 gtgactcttt gcaaaaagtc ttccgcttcc ccaaaatcag tgagttgacc tccacttata
    16981 tttataatgt gagctcctga actaactgct tgctcaatag cccgggacaa atcaagctga
    17041 gacagccttt gaccccgatc cgcaaagaca ggaacgatca acccacggca cgaaggagca
    17101 atgccttcaa ctggagaacc atgttggcca aaaattatac tgctaacatg agtcccatgc
    17161 tttgacatat ccccctgtga agttgcttcg ccatctacta aagttggcag acggcttaga
    17221 tgcgctcctt gaaaacaggg atgtgacttg tcaaccagtc catccaaaac cgctacgcaa
    17281 atgcgagggt cccctctggt tttcctccat agctcttgta tccccagtat ttccgttatg
    17341 tctgacattc atgttgaata tgagatattc tcattacagt tttatctcaa ttaggagtgt
    17401 atctcaaaaa aactcttgcc gagcatttaa ggacaaaatt gtaactattg ttaacaattg
    17461 gggagcttcg ttacttttta tactaaatct ggttatagcg tttcctgttc gcacgaggta
    17521 cattattgat ggggaaaagc ttaatcagca aaggtttaac tgtgccacac aggtatgata
    17581 atgggactta ggcgttttcg gtcagcaaaa aaggctcaaa ccattacggg acaaagggtt
    17641 aacctcgatt gatgattttc cttgatctat ctgagtttca gcgattttgg gcttctcgaa
    17701 gtaaatccca agcggggatt ggagttgagg cttttctcga tttgtttttt gtctaagtca
    17761 caataactgc tatattaaaa actgattatt tattcccctt tgccttttgc ctttcctcat
    17821 aactagcctg tactcaacgg atttagtatt attccacatt ccgcaccctc aactgtcata
    17881 taaggtaatc tactcccgtt aagccgatgc ttgaacggga gctttctgta accctgaatc
    17941 tatcggacaa gccaataaat tcggatttct gggctggatt acagaacaaa ccgcacaaaa
    18001 aatggatgac cgatgccctt attgactatt tgattgcgtt ctccccaatt aatgccatca
    18061 acagaagtat aaaattcagc tcctgaacag ctatttggac tcagcccaac aggacaggag
    18121 tatctaagca taatttcacg gtctcctgta aaataaatca gccgcccctc tgaacccttg
    18181 gtaatccctg gataatcttg tatccagaac cgtccttttt cacgaggacc tatgaattgg
    18241 gtaggagcaa caatataatg accttcgttg gcttcataac gtaccagctg tattggtaag
    18301 atagggcttt tattttcgac agtgacataa caggaataat cttgagacaa tccgggtatt
    18361 attactgatg tctgttctgc actatcagct tcaatctgat aagaaccctg ttggttaacg
    18421 actttgaata tcgccccttg atagctatcg gtatcaatct caatgaaatt aacatgctgt
    18481 ttgccgatat caggttttga aggacactca aatccgctgt tgacatattg aattaaaccc
    18541 tttttcagcc cagagatcgg cgtgtgtgta tgtcccatga ctactagatc tgccccgcat
    18601 tcgagggcaa gtttttgcgc aaaccacccc atatagtagt ctttagcatc agcaagagca
    18661 gctttaatag ctactagtag cccgtctcga gctccaccat tgttatttat ccattgctgc
    18721 cacaactgac tataaagtgg cttgacttca gctatcgtgg tcgtttgacc atcaggcagt
    18781 attatgggct gaatttccgg cattttagta gattgggtga tatagtttag cagcagatcg
    18841 gttacagaaa acccagattg tgctgccatg atagttgtcg ccaagctttt taggtcgata
    18901 ctattaggag aggtttgacc cgttaaatca gcaacagttt tacccggtgg gagagtctgc
    18961 tggagttcgt aagcagcggc acggctaaca aaatgtccga ccggtaaagg attgaacttg
    19021 acactagtat ccggcgcgtt gaacatcgcg tacagatgtc catgggtaca aacaattttc
    19081 ttattgtcct ctcccagagg gaagtaaata tcgctgggcg ataatttgat tgtgtagtca
    19141 ggattttgaa ttttgtttaa gtcctcttgg gtaacgttca tgtcgtgatt accgcgaaca
    19201 taggtaactt gtccctcaag tgcggttaaa acttgactta gctttccctg cggacctaaa
    19261 atatttgggt tctgttcgat gatttgctca aagcttggtg ggcgcttatc agggggataa
    19321 gtccagaaat caaatatgtc acccaaaaga attagttctt gaatggagct ggcatttttg
    19381 ataacgtgat ctaagactgc tgttaagtaa ggctcgtgaa agcttttttg ataccaaaca
    19441 gtgggactgt tgtctccaat gtgaatatca ctcaaaacta taattttatt ttttgacatg
    19501 gctcttttca ggactcctat tttgaatatt ttttgatcca acatctgttt tatttctgta
    19561 gcagactgtt gtaactgcgc caattcaaaa atgaaacgca tcaaccttaa gagttgacaa
    19621 agaaaggctg ctcaggtatg ctgatcgccg gtgtgatcct ggccatcatg ctcgccatca
    19681 accttaagag ttgaccaagg aaggcttatc aggtactgtc tgatcactaa taaaaacaaa
    19741 tcggacccaa aaacgggaca gcaacatctt aacatagaac aaagaaaatt gctatatcaa
    19801 cttagtcagg agggaaattt atctggctct tcggtaattg ttatgcaatg gacgggggtt
    19861 ataagggatg gaacacttat atagaaaggc atttagcgat ttttgtcaat tgtttttgat
    19921 ctagagcgaa ctaatcaatt aagtctctta ccagataagg atttagccga tttatgcccc
    19981 cctatcgaac cataccaagt aacgaagaac cagaaatggc ttatcttggc aggctcaccc
    20041 gatatacatt tagtggttgg aggtccgccg tgtcagggtt tttcggtcgc tggtaaacga
    20101 gatcccaatg atcctcgaaa tcaattattt aaagagttta taagagtggt ttctgaaatt
    20161 cagccttggt atgtcgtcat ggaaaatgtg ccagggattc tcacgatgca aaaaggcaag
    20221 gtgaaagaaa ctatcttaga agagtttgaa tcgatcggtt attctaatat ttcgatcgct
    20281 attcttgaat ctgccgctta tggaattcct caaattagac cgagagcaat ttttatagct
    20341 aatagattcg atctacctaa tccttatcct caacctcaat tactacctca tcaatatatt
    20401 ccaatagagt cggctatttc cgatttacct gcttacacgc ctattcccga aattaatcat
    20461 gaatggacac gccattcctc gaagtatatg gaacggatcg cacgagtacc ccccggtggt
    20521 tctctctatg aaacctacgt tgatgctttt aagcgccagt atccaggcaa accgagtatg
    20581 accatcaaag aaaatcatgg aggtacacat attcatcccc atcttaatcg agttatttcc
    20641 gcccgtgaaa tggccagact tcaatttttc cccgattcgt ttatttttga gggaaccatg
    20701 aaaaaagcta tgtggcaaat tggtaatgcg gttcccccac gtttagccga atgtatcgga
    20761 tacgctttaa ttccttatct caacaaagtt gctttacagc tagagcaaga atcgcagatt
    20821 aatcagaatt taaatccgat agcttgcggt tgagagcaat tctccatttc tgcaaatcat
    20881 accatccaca gccactacac cctacttccg ccacttttcc actttttgga atggtatccg
    20941 gccatttttc atcaccctca tcgaaaattt gggtctgaaa ccccgtcgta gaacgacggc
    21001 tttactgtta aatattagca catttacgaa atatatgcta aaatatgaga catggaaaaa
    21061 gcctattcgt ttcgatttta cccaacaccc gaacaagagt cgctattgcg gcgcacattg
    21121 ggctgtgtaa gattagttta caacaaagct ctccacgaac gaacacaagc ttggtacgaa
    21181 aagcaagaaa gagtaggata tgctgaaact tcttcaatgc taaccgattg gaaaaaacaa
    21241 gaagaattag actttttaaa cgaagttagc tgtgtacctt tacaacaagg gttaagacac
    21301 ctacaaacag ctttcactaa cttctttgct ggtcgtaata agtatcctaa ctttaagaaa
    21361 aaacatcagg gaggaagtgc cgaatttacc aaatctgctt ttaaatttaa agacagacaa
    21421 atctatttag ctaaatgcac agaaccttta cctattcgat ggtcaagaca aataccagaa
    21481 agctgtgaac caagcacagt aacagtcaga ttacatcctt ctggacgttg gcatatttca
    21541 ataagatttg atgacccaac aatccagcca ttacccccaa ctgataaagc catcggaatt
    21601 gacttaggaa ttagtagcct cgtgattacc agcgatggcg ataaagtgtc taatcccaag
    21661 cattttaaga aacattatcg gagactgcga aaggcccaaa aaagcctttc tagaaaacag
    21721 aaagactcaa aaaatcggga aaaggcaaaa atcaaagtag ccagaattca cgctcaaatc
    21781 accgatagta gaaaagacca tttacataag ctaaccactc aattagttcg ggaaaaccaa
    21841 acgattgtgg ttgagaattt agctgtcaag aatatggtca aaaacccgaa attatctcag
    21901 gcaatatctg acgttagctg gggagaaatc actcgacaat tagcctataa atgccgttgg
    21961 tacggcagaa attacatcga aatagataga tggtttccta gctctaaaag gtgtagtaat
    22021 tgcgggtata ttgctgagaa aatgccgtta aatgttcgag aatgggattg tccagactgt
    22081 gggacacacc atgaccgaga tgttaacgct agtaaaaata ttttggccgc agggcttgcg
    22141 gtgtcagtct gtcgagcgac cataagacca gaacagagta aatctgttaa ggcgggtgcg
    22201 gaaccccgca agggaaagaa gcagaaacct aaatcgtgag gtttgggaat cgccgtccgt
    22261 ttacggcggt gaggatgtca aaatagtatt ggataccgaa gggatagccc cgatcccctg
    22321 tttggtagca tttacggcag acttcacgct tctgtaaatt gcgctgattc gtcaaaagtt
    22381 gaaattgttc tcgaatctcc gtatcggcca aatgctctaa tgaatctcgt ctcgtatcgt
    22441 ttccccaacg aatttctgga aatttatggt cgggtaacag attatctttc cctacagtct
    22501 taccctcgta agcatcataa ccaccgagca gatcaatgat tttctttcgt agggatgacg
    22561 accatacttc gtatccagat attccaccac gaggtagagg aacaagtata atatgcgttt
    22621 tcttggaacc acaagtcggg catttttttg atgtatgagt tgcgatcgta tatcccattt
    22681 cttttaaatc ttgaatcctt ctagcccaat tcggattcgg gggtaattga caagcaacac
    22741 aagtccattt aaaagtgatg agtacatcaa agaatgattt tgttacttcc gcttctttct
    22801 gtaaccagaa gacctcttgt tcttgtctcc acttcggata attactcgga tgacagtata
    22861 gaaaagcttg ctgaagatat tcttcgattg ccgaagattc ggccagatct acgccagtac
    22921 gtctatactc aactggaatc gcgccttccc aaatcgtacc gtcatcatat cgaaaagtaa
    22981 catctatgta actttcggaa gcttttgagt gtctacgttg tccgtgttgt acgatctttt
    23041 ccattggttt gattaacttt gttttccaag ccttctaata gattggagac attgaggttc
    23101 attccgttcg ccagactaac aagagcggtt acagaaggat tgcgaatccc gcgctcgatg
    23161 ccagaaatat atgttctatc tagattcgag cgtgtcgcta gttcttcttg ggaaatacct
    23221 agattttctc tgtgttgtct tactaacgaa cccaacgctt ttaaaatttt tgccttgttt
    23281 tcgtccatag ctctatcctg accgaacata gacgatgagt ccacggacga tctgtcacat
    23341 tccgagcgaa gcactagaaa tcgagcggct tttgagtatc gcttgcccgt ttatatctga
    23401 taatccatga cgaaaaccac cttaccaccc cgcgcgttgg cacttaaggc agcggtcatc
    23461 ttcttgggat aggggagatt gagattattc attaactgaa taaaatcctc gcgattgcgg
    23521 ccggcaaaac ggggattcca gtgcttttcc tcaccgatcg aggagaccgt tcttcccaga
    23581 taatcgtgac agggataaac aagcgtatcg tcggggagag tgaataactt ctccgttacc
    23641 gtcttgtata gcacctccgg cgaaccgttc tgaaaatcgg tacgaccgca accgcgaatt
    23701 aagagtgcat cccccgttaa taggcgtttt tcatcgatga gataggctat atgactatcg
    23761 gtgtggcctg gagtggcgat cgcttttagc tgctgacctg ccacggtcca gaggtcgcca
    23821 tcgcgcacat aaccatcgat atcgctcact tccgcgtttt cgggaacgag gattgaacag
    23881 ttggttaact cccgtaagcg gtgcgctccg gtgatatggt cggcgtggac atgggtttcc
    23941 atcgtgtagc ctagagttaa ccccaactgc caaagaatct gccgatcgcg atcaacctgt
    24001 tcgagaaccg gatcgattaa aattgcctcc cccgtacccg aatcagcgat cagataggtg
    24061 taggtactcg attccctatc aaaaagctgg cggaaaatcg gcgctgccgg ctggaatgcg
    24121 atcgagtagg ccgtggggag agaaattctc tctttcagcg atcgcaacaa atcccgggcc
    24181 agttcggccg cctgtaaacg cagttcggga atggcggtgg tttcccagag atcgcctttg
    24241 cggggatttc ccaaggtgta cagcgtgttc gatgccgtac cgtctggcct gagaatcgcc
    24301 ccgttgtccg cggtttcgat gccacaattc aaaggatggg gacggattaa gccccgttgg
    24361 cgaagatgaa cgacgagggg atcggtgatc gtctggtaat cgttactcgc gccggtacag
    24421 ttaatgattc gatcgatcgt taggtttaag aggtttcccg tcccccgttg acggatagta
    24481 acctctacac agccgttttt aacctcagca ctttcaattc ttcccccatg ataggttaat
    24541 tgtcgcgatt ctacagcctc atcgaggata ctggcgatct cgtcggcgag gcgatggcga
    24601 agaacctccc aataggcttt caggtggcgt agaaatctgg cgcgttcccc tatcggcaga
    24661 caatgccata gaccttgaga tatcgggcgt aaggcgttta aaacggcccg ccagtcgtga
    24721 ccttgactct cggcggtttt tacctcggcg cgaattcgcc gtaggagtcc ccgggtggtc
    24781 tggggcgcgg tttcgagggt gaggaacggg ggatagggat cggtgggtcg gtgcgatcgg
    24841 gggatcaggc catgacggga aaccgcgtgg attttgcccg tgaatcccct ttgtgccagg
    24901 gaaaccacca tatccaccat cgttaacccc gtaccgacga gcagaatcgt cccgtcgggt
    24961 tttagctcgg cgagtgtgtc cgtctcccaa gcgtcgcgga gatagaggga attgagagac
    25021 gcgaggggtt ggggaaccgt tgcgggaaaa ttgcccagcg ccagaactac tttagcggcg
    25081 ctgattttct tgcctccttt gagggtaatc gtcgcctttt ccccgtcgag tgccagatcg
    25141 atcgccgcat cggtaaacgt ttccagacgg tgatcggcaa tggcgttgtc ccgcgcttct
    25201 tctaggatcg atcggatgta tttgccgtac actagacgcg gtacgaagct ggccggctcg
    25261 atcgatcgat agccgttatc cgctaaccag tggaggaaat gttcgggatc gtcctcgaag
    25321 gcgctcattt tgcccgcagg aatattgagc aggtggccgc tgtctcgcgt tccgtaggcg
    25381 atccccgtac cgaggggttt tcggcgctcg atcagggcga tcgataatgg agtccccgtg
    25441 tcgcgtaaca ggttcgccgt cacgagggaa ccactaaatc ctcccccgat aatggcgatg
    25501 tcgaaagttg cagaaaattg attccggtca atgtagctca tagataactt ttaaagagga
    25561 tttagtatta gttagtctag ctgaaagata attttggttc ttcggtttgt gttatgcaat
    25621 agacgggggg tataaggtat gtaaccctta tagagaaaga catttggaga tttttatcaa
    25681 ttgcttttgc tctagagcga actaatcaat taagtctctt gccagataag gatttagtcg
    25741 atttatgcca cccgatcgaa ccataccaag taacgaagaa ccataatttt ttaggcattc
    25801 ttggttaata ggttaaaaat aaacgataga attggctatc aattaagata taaataccga
    25861 gagcaatata gataaaagga ctaattttat gtccgtagtt tgtaaccact ttagctatcc
    25921 tgggatgggt aatcaaaaaa taggctaggg cgcaccaaat acccatcatg agataaaaaa
    25981 ctatcatgat aattaagacg tgcatcgggg aattattggc aaaaagagag gtatagatac
    26041 caatattatc accaccatta gccacggtca cggcggcaac gtaataggtt tgggagggaa
    26101 aacgaggaat aaaggagcga tttttagaat aattaacatc ttctcttact tcttgaatgt
    26161 aatcctcatt ttcccttgac aaaagttgtt taatgccgat aatcaaagga ataaatccta
    26221 atagaccaat ccattcttta gaaaccagta acccaaatag taaaccgagg gaactagcta
    26281 ggagaatgag ggtaaaaccg aggtattgac caataatcag gtgtcgaggt cgaaaattag
    26341 cattaagtcg cgagaaaaac aacattagca ccatcagatc atcaagattt gtggctacaa
    26401 aactcgtcac gctagtaatt aaaagggcta ctaaccaatt catagattga aaaaagagat
    26461 aatccttccc attctaaatc tcgtagtgcc agatttcctc ctctcgtaaa agaagacgat
    26521 ggacgggaag acgttccaca gccccctgtt gttcaagatg attaagtgcg cgggtaattg
    26581 tgactctagt agttcctaat aagtcagcga tatcctgatg agttagacgc acatcaatta
    26641 attgtccggt accggtttgt ttaccaaatt gattgcctaa ccatgacagt aatttcacaa
    26701 ccataacatc agtacgtttg tagctacgaa taatacttag ttcttgtgct tgctgaagat
    26761 gagacagcag aatttctgcc atttgataac tctcaactgt ggaaaaggaa actgcttgta
    26821 caggagtgat gcactcaact tgatagggat tgactctctc tagggttttg ccaacaaaat
    26881 tgccaggtcc ccacaatccc aagacgatca gggttccatc ttctaaccag gtggaggttt
    26941 tcacgactcc actctcgatt ttccagaaat tagggtttct gaggggcaaa atcgcccgat
    27001 tatcaaagtg ccagcgatgg ggggttgatg gttgataggt aagcgtcatg attgataatt
    27061 aattttgatt aggtacttag ctagtcaaaa gcacaaggcc gtcgagaaag gagcttttta
    27121 gacgagttaa ggtttttttg aagaattttg gggtaaaaac gataattacc ccaaaaaaga
    27181 ccgattttat gcccttacaa gccctattta tttcggggtg ttagtcttct ccagttccgt
    27241 tgtggccgag gagatcagtt cttgccaagc tcgatcgctt tcctgtagag tttgtttaac
    27301 cgcgtcgatg gtcttttcca cgcgagtttt caggaaaggg gaagcact
//
