LOCUS       NZ_AP024565            20153 bp    DNA     circular CON 26-SEP-2024
DEFINITION  Microcystis aeruginosa strain NIES-88 chromosome, complete genome.
ACCESSION   NZ_AP024565
VERSION     NZ_AP024565.1
KEYWORDS    RefSeq.
SOURCE      Microcystis aeruginosa
  ORGANISM  Microcystis aeruginosa
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Chroococcales; Microcystaceae; Microcystis.
REFERENCE   1
  AUTHORS   Phan,C.-S., Matsuda,K., Balloo,N., Wakimoto,T. and Okino,T.
  TITLE     Argicyclamide A-C unveil enzymatic basis for guanidine
            bis-prenylation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5478552)
  AUTHORS   Matsuda,K. and Wakimoto,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2021) Contact:Kenichi Matsuda Hokkaido University,
            Graduate school of phrmaceutical sciences; Kita 12, Nishi 6,
            Kita-ku, Sapporo, Hokkaido 060-0812, Japan URL
            :https://www.pharm.hokudai.ac.jp/tennen/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 87x
            Sequencing Technology             :: DNBSEQ; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019704275.1-RS_2024_09_26
            Annotation Date                   :: 09/26/2024 11:16:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,602
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,071
            CDSs (with protein)               :: 5,071
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 110 of 479
            Pseudo Genes (incomplete)         :: 380 of 479
            Pseudo Genes (internal stop)      :: 101 of 479
            Pseudo Genes (multiple problems)  :: 91 of 479
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 14:54:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2949410
            Orig. end                         :: 2969563
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024565.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated at DFAST https://dfast.nig.ac.jp/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20153
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="microviridin"
                     /region_number="9"
                     /rules="(mvd or mvnA)"
                     /tool="antismash"
     cand_cluster    1..20153
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(mvd or mvnA)"
                     /kind="single"
                     /product="microviridin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20153
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10153](+)"
                     /cutoff="20000"
                     /detection_rule="(mvd or mvnA)"
                     /neighbourhood="10000"
                     /product="microviridin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10153
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(mvd or mvnA)"
                     /neighbourhood="10000"
                     /product="microviridin"
                     /protocluster_number="1"
     gene            830..1129
                     /locus_tag="LC603172_RS14745"
     CDS             830..1129
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002746002.1"
                     /locus_tag="LC603172_RS14745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_155726564.1"
                     /transl_table=11
                     /translation="MVISVISYQLSVISYQLSVISYQLSVISYQLSVISYQLSVISYQL
                     SVISYQLSVISYQLSVISDYQQSLVAFNFHSLERGGLLPLVFGHISRWVELNIR"
     gene            1485..1661
                     /locus_tag="LC603172_RS14750"
                     /old_locus_tag="MAN88_28600"
     CDS             1485..1661
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002740449.1"
                     /locus_tag="LC603172_RS14750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28600"
                     /product="DUF4089 domain-containing protein"
                     /protein_id="WP_002740449.1"
                     /sec_met_domain="PP-binding_2 (E-value: 0.0044, bitscore:
                     15.6, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNPEEYVIIMAKILDLTIPDRYLNSVVENWQRLQEIASLVTEFP
                     LEDDGESALSFEP"
     gene            1658..2989
                     /locus_tag="LC603172_RS14755"
                     /old_locus_tag="MAN88_28610"
     CDS             1658..2989
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1105: amidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004158179.1"
                     /locus_tag="LC603172_RS14755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28610"
                     /product="AtzE family amidohydrolase"
                     /protein_id="WP_061430263.1"
                     /transl_table=11
                     /translation="MTISVSNLARSIQNRQTSALEVINRTLAEIEAKNRHLNCFTDILH
                     PRALAQAQKIDLAIANGEPVGVLAGVPFAVKNLFDIEGIVTLAGSKINRDHPAAGQDAI
                     AIQTLEAAGAVLVGATNMDEYAYGFVTENAHYGATANPRDPSRVSGGSSGGSAAAVAAN
                     LVPLALGSDTNGSVRVPAACCGVVGLKPTFGRVSRQGLFLFVSSLDHPGFFSDNVADMA
                     AIWSLFAKNNLKAPLSGLEGVKIALADDYFQQGAEPEVIESVIAIAERLGVTKKITIPE
                     TARARAAAYIITASEGANWHLPRLQTRLEDFDPATRDRFLAGALIPSSWYLQAQRFRRW
                     YRDRLREIFSEVDIILTPTIPCIAPPLGVEKMIIDGQELLIRPNLGRFTQPFSFIGLPA
                     LSLPIKRSSRLPLGLQIIAAPDREDLILRVARVLEEMLIDIPHK"
     gene            complement(3315..4484)
                     /locus_tag="LC603172_RS14760"
                     /old_locus_tag="MAN88_28620"
     CDS             complement(3315..4484)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002801155.1"
                     /locus_tag="LC603172_RS14760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28620"
                     /product="glycosyltransferase"
                     /protein_id="WP_061430264.1"
                     /transl_table=11
                     /translation="MSQATPSLQSNLKQWLRASAPSLFQIKQRLSARRWPKKSIVIYTE
                     KYRPMRPAEIWQRGASGTQASVAFLAQEWTKRGYKVTIFGNCEGMEGEQDGIRYVNYYH
                     MNWQDHFETLVISRHPTYCRPGTKAERIFFDWQDIKVPADSYTPEILARYDKIFCKSQY
                     QRDLLDFCADNQCPIVPNGIDTSLLAISDTVPESEYIPYRLVYASRYYRGLESMLKHGW
                     PIIKAAIPAAELHLYYGFTKRDGIGDRKEWRDNLIALIKASPGVVDRGMMGYRQLIEEK
                     ARSAIHYYGCTYREIDCISVRESAVVGCLPVVTDFAVFREKDYCVKVPGEVTDPATQEA
                     IAWKIVELLKNPQLLAQERQRCRQIALTDTWERSAAAWLDVIEKESPSQ"
     gene            4651..5928
                     /locus_tag="LC603172_RS14765"
                     /old_locus_tag="MAN88_28630"
     CDS             4651..5928
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771804.1"
                     /locus_tag="LC603172_RS14765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28630"
                     /product="glycosyltransferase"
                     /protein_id="WP_061430265.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.8e-17,
                     bitscore: 61.8, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIVIPILFYSKGGVERVIISLIPSLLEYVEQIIIVLPRKEIEYF
                     RYLMPDSDKLIYEDLAFYPQSFETKLIYFYVLLANFYKTLRLNALHRLFSRRIELLRIE
                     ARINQIVKRYQADHCLYGMTNRISPPNVKVTLSGIIYDVFWQFAPLTYSESYQEPYNEA
                     LKEWLDRADLLFTISQKTKDDVLKVFPNATYASKLKAVPLAGLIEQSNPKIDLVKSELV
                     TFYFPSSFGIYKDHLTLLKAAITLAKKGLKFQIVFIGKETDNLVNGNLQLSQQSQTQEY
                     QDYLKECNRVYRENQEIFESHIKGLGYQDYETLQFYYQTCSSVVFPSKYEGFGLAIAEA
                     ILQGIPVIASDLEVFQEQVELYNCPERVQFYPRGDAESLANCLEQFILNPIPRLLPEDI
                     PNKINLWTWEDVAKKYVELLKENAGY"
     misc_feature    complement(4669..4685)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 25.1"
                     /tool="antismash"
     gene            5915..6706
                     /locus_tag="LC603172_RS14770"
                     /old_locus_tag="MAN88_28640"
     CDS             5915..6706
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004158181.1"
                     /locus_tag="LC603172_RS14770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28640"
                     /product="MOSC domain-containing protein"
                     /protein_id="WP_061430266.1"
                     /transl_table=11
                     /translation="MQVTDLYIYPIKSCRGIQLSQAEVTPKGLLWDREMMLVDGKGKFI
                     TQREYPQLATVSVMMTEDNFSLKTSQDSLTFQPNFTGEKRAVQIWESHTIAMDQGDEIA
                     EWFEKILDIPCRLVRQSPDYPRPVNPRYAGNDHTPVSFADGYPILLTNTASLEDLNRRL
                     VTPVPMNRFRPNIVISSDRAFSESSWQKITIGEINYALVKPCSRCIITTTDQETGRRNP
                     QQEPLKTLSTFRSFPGGIMFGENVIPEKTGTIKVGDPITVI"
     gene            6912..7973
                     /locus_tag="LC603172_RS14775"
                     /old_locus_tag="MAN88_28650"
     CDS             6912..7973
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1157: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002740457.1"
                     /locus_tag="LC603172_RS14775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28650"
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="WP_061430267.1"
                     /transl_table=11
                     /translation="MPLDTASILEVLRPVQDPELQKSLVELNMIRNVAIDGGKVSFTLV
                     LTTPACPLREFIVEDCQKAVKQLPGVESVAVAVTAETPQQKALPDRQGVEGVKNIIAVS
                     SGKGGVGKSTVAVNIAVALAHLGAKVGLLDADIYGPNAPTMLGLNDAQVTVQGANGEIL
                     EPAFNHGIKMVSMGFLINPDQPVIWRGPMLNGIIRQFLYQVNWGDLDYLIVDMPPGTGD
                     AQLTLIQSVPLAGAVIVTTPQTVSLIDARRGLKMFQQLGARVLGIVENMSYFIPPDQPD
                     RSYDLFGSGGGEKTSQELGIPLLGCVPLEISLREGGDTGVPVVLGQPESASAKALIAIA
                     RQVAAKVSVAAYS"
     misc_feature    complement(6948..6957)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 16.53"
                     /tool="antismash"
     gene            complement(7970..8104)
                     /locus_tag="LC603172_RS28205"
     CDS             complement(7970..8104)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002740459.1"
                     /locus_tag="LC603172_RS28205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002801152.1"
                     /transl_table=11
                     /translation="MKSKSTGHWSDWGENLGTFSLKMGKTLHPTPYTLPLGKTFCRKP"
     gene            8185..9465
                     /gene="rodA"
                     /locus_tag="LC603172_RS14780"
                     /old_locus_tag="MAN88_28660"
     CDS             8185..9465
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771808.1"
                     /locus_tag="LC603172_RS14780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28660"
                     /product="rod shape-determining protein RodA"
                     /protein_id="WP_061430268.1"
                     /transl_table=11
                     /translation="MVRRQSFISRKLRGFSERFSFIFRDLAQMDRLLFVLVTGLTVFGG
                     LMIRSAERHTTALDWWQHWLFGAGGVAIALFLARCRYESLLKWHWLTYLLTNLSLIAVI
                     VLGVTANGAQSWINIGSFNVQPSEFAKVGLIITLAALLHHRPADNLFAIARVFVVTAIP
                     WVLIMAQPDLGTGLVFGAITLGMIYWANAKLPWMIILLSPLASVFLFNLLFPAWIVLAI
                     IIAVLAWFTLPYRFLSTFIVVATNLAVGKLGEVFWGLLKEYQKDRLTLFLHPEKNPLGG
                     GYQLIQSRIAIGSGELLGRGLHQGTQTQLNFIPEQHTDFIFTVVGEEFGFVGSILVLIA
                     FWLVCWRLLVIANTAKENFGSLIAIGVLSMIAFQAILNISMTVGLAPITGIPLPWLSYG
                     RSSLLTNFIALGLVESVANYRPRKRLY"
     misc_feature    9799..9813
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.63"
                     /tool="antismash"
     misc_feature    9875..9888
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.16"
                     /tool="antismash"
     gene            10001..10153
                     /locus_tag="LC603172_RS14785"
     CDS             10001..10153
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     microviridin: mvd"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     microviridin: mvnA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF023973.5"
                     /locus_tag="LC603172_RS14785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="microviridin/marinostatin family tricyclic
                     proteinase inhibitor"
                     /protein_id="WP_008201950.1"
                     /sec_met_domain="mvnA (E-value: 3.9e-28, bitscore: 95.6,
                     seeds: 16, tool: rule-based-clusters)"
                     /sec_met_domain="mvd (E-value: 2.1e-13, bitscore: 48.7,
                     seeds: 55, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAYPNDQQGKALPFFARFLSVSKEESSIKSPSPEREYNVTLKYPS
                     DWEEF"
     misc_feature    10412..10425
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.17"
                     /tool="antismash"
     misc_feature    complement(10421..10437)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 23.91"
                     /tool="antismash"
     gene            10432..11412
                     /locus_tag="LC603172_RS14790"
                     /old_locus_tag="MAN88_28670"
     CDS             10432..11412
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002801150.1"
                     /locus_tag="LC603172_RS14790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28670"
                     /product="MvdC family ATP-grasp ribosomal peptide maturase"
                     /protein_id="WP_061430269.1"
                     /transl_table=11
                     /translation="MKESAKVVLLLTHSGDFFTIDRVAEAIEKKGATPFRLDTDKFPLE
                     VQLAAQFNGKKSFYQLTYNHQSIDSQQVQSVWTRRIWQPEITGDLDPQFREACVRESQT
                     TLAGFWDSLRSARWLDNLAQIERAKNKLLQLRLASEVGLIIPPTLVTNNPDAAREFFSQ
                     VQGRMVSKLLTAIARSMESPEFFLYTSRVKAEDLEEAESLRYCPMVFQAEIPKQLELRI
                     VVVNGQTFVGALESSQYNNSAVDWRRPGIDPGAWQHHTLPDSLLQQLQIFMANIGLNFG
                     AFDFILTPGGEYVFLEVNPCGEWGMLERDLDLPISHAIADFLVFG"
     misc_feature    complement(11428..11443)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.66"
                     /tool="antismash"
     gene            11466..12440
                     /locus_tag="LC603172_RS14795"
                     /old_locus_tag="MAN88_28680"
     CDS             11466..12440
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517285.1"
                     /locus_tag="LC603172_RS14795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28680"
                     /product="MvdD family ATP-grasp ribosomal peptide maturase"
                     /protein_id="WP_061430270.1"
                     /transl_table=11
                     /translation="MTVLIVTFSHDNESIPLVIKAIETMGKKAFRFDTDRFPTEVKVDL
                     YSGGQKGGIITDGEQKLELKEVSSVWYRRMRYGLKLPDGMDSQFREASLKECRLSIRGM
                     IASLSGFHLDPIAKVDHANHKQLQLQVARQLGLLIPGTLTSNNPEAVKQFAQEFEATGI
                     VTKMLSQFAIYGDKQEEMVVFTSPVTKEDLDNLEGLQFCPMTFQENIPKALELRITIVG
                     EQIFTAAINSQQLDGAIYDWRKEGRALHQQWQPYDLPKTIEKQLLELVKYFGLNYGAID
                     MIVTPDERYIFLEINPVGEFFWLELYPPYFPISQAIAEILVNS"
     gene            complement(12728..14791)
                     /locus_tag="LC603172_RS14800"
                     /old_locus_tag="MAN88_28690"
     CDS             complement(12728..14791)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002801146.1"
                     /locus_tag="LC603172_RS14800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28690"
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="WP_061430271.1"
                     /transl_table=11
                     /translation="MPQYTTKQATENPVVSDQSANNPFSLFGQFWESLKIVAQPYWYPT
                     ELNGRAFGDVIISWGMSALILLSILATVGVEAFSSYWNRYVLDIIIEDRDLSKYLNTLW
                     LSSLLIILTTGLFAFSQFIRRQVALDWYKWLTKQTVKKYLNARAYYNIDFTSALKNPDQ
                     RLSQEIEPITTMTLRLLITFVEKGLQMITFAIILWTISRQIAVYLIIYTIAGNLIAIYL
                     TQELNKINQSELNNKADYNYALTHVRNHAESIAFFQGEEQEEKIIEGRFERVIQSSEKL
                     INWERFNNLFNRGYQSAISVFSMFILTPMFIKNEIDYGEISQASLCCFLFSNALGQLIT
                     EWGTSGKVSSYIERLATLTDALKVASEEPKHLSRIITLEDNRLAFENFTLQTPDYEKVI
                     VENLSVSVPLGESLLIIGPSGRGKSSLLRAIAGLWKAGSGRLVRPPLAKMLFLPQRPYI
                     ILGSLRQQLLYPHTNDQVSDEELENILKKVNLQHLLTDKNTLDKQVNWEQILSLGEQQR
                     LAFARLLITKPTFTILDEATSALDLANEASLYQQLQESETTFISVGHRESLFNYHRWVL
                     ELTENSHWQLSTVEDYQRKKVNNLVMMSKKSENFSAVKIETLSEVKTEHDAEIAGVSEI
                     AEGLSHQQMQSLTDYSLSNVRSRASLGKIITAKDGFNYRYDKDPKTLKWVRI"
     gene            complement(15011..15265)
                     /locus_tag="LC603172_RS14805"
                     /old_locus_tag="MAN88_28700"
     CDS             complement(15011..15265)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775794.1"
                     /locus_tag="LC603172_RS14805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28700"
                     /product="hypothetical protein"
                     /protein_id="WP_002758977.1"
                     /transl_table=11
                     /translation="MEKFNSKDNFLYPKTSYRGLFTPQNLVFNANLQEFALRVSFIAGL
                     HSGGKLTSTEAYEKINQLWQELNTSQKFLLANHFEDDGD"
     gene            complement(15364..16308)
                     /locus_tag="LC603172_RS14810"
                     /old_locus_tag="MAN88_28710"
     CDS             complement(15364..16308)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517282.1"
                     /locus_tag="LC603172_RS14810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28710"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_061430272.1"
                     /transl_table=11
                     /translation="MTNFSIKVGIIGAGLAGLTCARQLCDRGYTVKLFDKSRGIGGRLA
                     TRRVNRANQEMAVDHGLPFLTVQGEKTAALIDNLLRENLVTSWFDSSYVAPSGINSVAK
                     FLAKGLEIERDFLVTRLENRQEKWVLNNNGQIRGEFSAIVLAIPAPQAALLLENSPITT
                     MPELRSIVYDPCLTVMAGYGDSLPAVAPSTDIAWLGLDSSKRQSSPDYVFVVHSSGDFA
                     VKYLDSEDLEAVKLDLLSRASLPLPDWSQLHRWRYALVRQGLAVPCLSVNSPLPLVACG
                     DWCQGGDLSRNSSLETALTSGIAAANQVQQLLS"
     gene            16406..17233
                     /locus_tag="LC603172_RS14815"
                     /old_locus_tag="MAN88_28720"
     CDS             16406..17233
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002740471.1"
                     /locus_tag="LC603172_RS14815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28720"
                     /product="YaaW family protein"
                     /protein_id="WP_002758975.1"
                     /transl_table=11
                     /translation="MDELRTALELATEEELQQITNILFCRRFNPLDYLRAPDAIAVQSQ
                     DWDSWLDSVEDRFRYLAADGVTVLKGQTEKVSYRQILVRVCHFLKVPYSQKMPTTEIEA
                     EIYLHLVNKAWKRLPPSEQKSLSIQIQKALADSHTPQPLPVHLQHNPLDIVLKGGSVIA
                     INSILKPILLKHIAGQFALHFARYQGAKTALVQGGAIANQLALQTAKQGMTTAAARYGA
                     VRTVLSLVGPALWGWFIADLGWKAIATDYGRIIPTIFAIAQIRLTRDDCWQPA"
     gene            complement(17277..18464)
                     /locus_tag="LC603172_RS14820"
                     /old_locus_tag="MAN88_28730"
     CDS             complement(17277..18464)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1192: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517330.1"
                     /locus_tag="LC603172_RS14820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28730"
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="WP_061430273.1"
                     /transl_table=11
                     /translation="MEKAYSFRFYPTPEQESLLRRTLGCVRLVYNKALHERTQAWYEKQ
                     ERVGYAQTSSMLTDWKKQEELDFLNEVSCVPLQQGLRHLQTAFTNFFAGRTKYPNFKKK
                     HQGGSAEFTKSAFKFKDKQIYLAKCTEPLPIRWSRQIPDGCDPSTVTVRLHPSGRWHIS
                     IRFDDPTIQPLPPTDKAIGIDLGISSLVITSDGDKVSNLKHFKRHYRRLRKDPKSLSRK
                     QKGSKNREKARIKVAKIHAQITDNRKDHLHKLTTQLVRENQTIVVENLAVKNMVKNPKL
                     SQAISDVSWGEITRQLAYKCRWYGRNYIEIDRWFPSSKRCSNCGYIVEKMPLNVREWDC
                     PDCGTHHDRDVNASKNILAAGLAVSVCRATIRPEQSKSVKAGAEPRKGKKQKPKS"
     gene            18555..19829
                     /locus_tag="LC603172_RS14825"
                     /old_locus_tag="MAN88_28740"
     CDS             18555..19829
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Wzy_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731610.1"
                     /locus_tag="LC603172_RS14825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_28740"
                     /product="O-antigen ligase"
                     /protein_id="WP_061430274.1"
                     /sec_met_domain="Wzy_C (E-value: 2.1e-14, bitscore: 52.2,
                     seeds: 81, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIFCQGWEWNLILFSVFIAMMLPELAGIGIIIVLIRVFTGNFR
                     AIIRSRLNQGLGIVTLWLMISASLSPTPGDAWIGLGNFLPFFFFFSNIRQLIKKPSQLR
                     QLAWAMVIPSVWVVVMGWGQIFLGWQKPEALKGIFTWPYGAGGEPLGRMSSVFMYANLL
                     GAYLLITLILALGLLILTWRQWHRQRNTLLNLKVGFLTLTIFIDSVGLFLSNSRNAWAL
                     AFLGGLIFALYLGWNALVAGFILLGGVISLASWGPNPFRDIVRFLVPRAIWARLSDEMF
                     PDRPLATLRSTQWRFTLEMTQERPLFGWGLRSFTPLYEKSQGLWLGHPHNLYLMLMAET
                     GIIGLILLSVWVGWIYAQGLRLFIFLRQQKAGGELIIFTYLVAFGACVLFNLVDVTLSD
                     VKINTIVWFLLAAIAGVSQRLNYEL"
     gene            complement(19824..20096)
                     /locus_tag="LC603172_RS14830"
     CDS             complement(19824..20096)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS14830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_221275337.1"
                     /transl_table=11
                     /translation="MHPYSGDYFIYSPHTPHPTPHTLLPHFPNSHTPHPTPHTPHPTPH
                     FPTSPLPQFPHPTPHTPHPTPYFPTSPIPTPHTLHPTPHTPLPNS"
ORIGIN
        1 ttgactgacg gaagcaccta ataaacccag gcgatcagtc acttttaaac ctttttcaat
       61 ggcgggaatt aaagattcta aactagcggt acgaaagggt aaagttagat aactagctaa
      121 acaaaaacgg cacatttctg gacaacttcg caccacctct accatataaa tattctccca
      181 tgccgcttgt tttgttacca ccgtagaagc agaaagggta ttactgcgat aggtttgttt
      241 actaacaaag gtaggaacat tattatctat gggcagaatt gccgtaattt cgccatcggg
      301 actatcataa ttaacggtat ataaactagg aacatacacc cccggaacct gggctaaatg
      361 acggagtttg gtcggtcgat cggcgtttct cacctcttga taggcattaa taaaatcagc
      421 aattaagttt tcaccatctc ccaagaaaat tacatcaaaa aaagcggcaa aaggttcggg
      481 attagcggtt aaaactggcc cacctccgaa aattatcgct tgcctatctt gccgttgatg
      541 actgtgacaa ggaatttcta aagattctaa cagcgagagg atattaacat agtctaattc
      601 ccaagaaaag gaaaagccga ctaattcggg ataacggggt aaagattctt gaaaatccgt
      661 aaataaacga ctaacttgta tatccgatcg cagggctaaa gttgcccaaa caatttgata
      721 acctaaactg gttataccca cagaatactg attaggaaag gcaaaaatag ttctaagggc
      781 atcactgtgg ggtattgcgg gagtaaagag gagtttttcg tcttgtaaga tggtcatttc
      841 agttatcagt tatcagttat cagttatcag ttatcagtta tcagttatca gttatcagtt
      901 atcagttatc agttatcagt tatcagttat cagttatcag ttatcagtta tcagttatca
      961 gttatcagtt atcagttatc agttatcagt tatcagttat cagttatcag ttatcagtga
     1021 ctaccagcag tctttagtag cttttaattt tcattctctt gaaaggggag gcttgttacc
     1081 tcttgttttt ggtcatataa gtaggtgggt ggaattaaat ataagatgaa cgtaggttgg
     1141 gttgaagaat gaaacccaac gcccgcatgg gttacgctac tgctaaccca tcctacaaat
     1201 aattgtgcct ccctacttat aagtttaatt taatcggggg gtgaagggga ggaaaatatc
     1261 tcaatataca gttctaggat catatctaag gttatgatcc tagggagtcg gtggtcaaga
     1321 acaaatttaa gttatacttc ctccctaggg atagctattg tatttacagg agattttctc
     1381 ttatatccta gcaattttca cagcttaact aggtgtcgat tttattcctg agcgctgctt
     1441 tcaagcaact ctttatctcc ttcaccccag ccggaacgaa atctatgaat aacccagaag
     1501 aatacgttat aatcatggcg aaaatcctag atttaaccat tcccgatcgc tatttaaatt
     1561 ctgtggttga aaactggcaa cgcttgcaag aaatcgccag tttagtgaca gaatttccct
     1621 tagaggatga cggggaaagc gccctcagct ttgaaccatg actatttctg tgagcaattt
     1681 agctcgatcg atccaaaatc gtcaaactag cgccttagaa gttattaatc ggactttagc
     1741 agagatagag gcgaaaaatc gccatttaaa ttgctttacc gacattttac accctagagc
     1801 gctcgctcaa gcccaaaaaa tcgatctagc gatcgccaat ggggaacccg tcggtgtctt
     1861 agcaggagtc cccttcgccg tcaaaaatct ctttgatatc gagggtattg tcacccttgc
     1921 cggttccaaa atcaatcgag atcaccctgc cgccggacaa gatgcgatcg ctatacaaac
     1981 cctagaagca gccggggcag ttttggtggg ggcgaccaat atggatgagt acgcctacgg
     2041 ttttgtcacc gaaaatgccc attatggggc cactgccaac ccccgcgatc ctagtcgcgt
     2101 ctcgggggga tcctccggcg gttccgccgc tgccgttgcc gctaatttag tgcctctcgc
     2161 cctcggttcc gatactaatg gttccgtgag ggttcccgcc gcttgttgtg gcgtagtggg
     2221 gctaaaaccc acctttgggc gcgtatcccg tcagggccta tttttgttcg ttagtagttt
     2281 ggatcatccc ggatttttta gcgacaatgt ggcggatatg gcggctattt ggagtctttt
     2341 tgccaaaaat aaccttaaag ctcctttaag tggcttagag ggcgtaaaaa tagcccttgc
     2401 cgatgattat tttcagcagg gagccgaacc agaagtaatc gagtcggtga tagcgatcgc
     2461 cgaacgttta ggagttacca aaaaaatcac cattcccgaa accgcccgcg ccagggcagc
     2521 cgcctatata attaccgctt ccgagggcgc aaattggcat ttaccgcgtt tacagaccag
     2581 attagaggat tttgaccccg caacccggga tcgcttcttg gcaggggcat taattccctc
     2641 tagctggtat ttacaggctc aacgttttcg ccgttggtat cgcgatcgtc tgcgagagat
     2701 tttcagcgaa gtcgatatta tcctcacccc aacgattccc tgtattgcgc caccgttagg
     2761 agtagaaaaa atgattattg acgggcaaga gctattaatt agacctaatt taggcagatt
     2821 cacccaacct ttttctttta tcggtttacc agccctttct ttgccgatta aacgttcatc
     2881 caggctacct ttaggtttac aaatcatcgc cgcacccgat cgcgaagact taatcctaag
     2941 ggtagccagg gttttagaag aaatgctcat agatatcccg cacaagtgag atttcaaggg
     3001 cttacttcct cttaaaatcc ctaatttagg cacttataag cgaaaatgtt acttttatag
     3061 tctgagtagg gttggctgaa taaatgtgaa atatagacga ggtaaggggt ttgagggaat
     3121 gtttggcgaa gcagctgcaa gattttcaag ctagtggctc aaaaaccttg catcttgagc
     3181 ggctggcgtc ctggtaggtc ttcaaataat atctgtaggg gcgaagcatt cgggcaataa
     3241 cctatcgctg aaaccgtaga ttttctatcc gaatgcttcg cccctacttt ttcagcaaac
     3301 cctaagtaaa caatctactg gctcggagac tccttttcaa tcacatctaa ccaagcggcg
     3361 gccgagcgct cccaagtgtc agttagggca atttgacgac atctttggcg ctcttgggct
     3421 aaaagctggg gatttttcag taattcgacg attttccagg ctatagcttc ctgagtagcc
     3481 ggatcggtta cttcccccgg cactttcaca cagtagtcct tttctcgaaa aacagcaaaa
     3541 tccgtcacca ccggcagaca gcctaccact gctgactcgc gcacagaaat acaatcgatc
     3601 tcacgatagg tgcaaccgta gtaatgaata gcagagcgag ctttttcctc gattagttga
     3661 cgataaccca tcattccgcg atcaactacc cccggactag ctttaatcaa agcgattaaa
     3721 ttatcgcgcc attccttgcg atcgccaatg ccatcccgtt tagtaaaacc atagtagaga
     3781 tgtaactcgg ctgcgggaat tgccgccttg ataatcggcc agccatgctt taacatcgac
     3841 tctaggccgc gataataacg agaggcatag actagacgat agggaatata ctcactttcc
     3901 ggcactgtat cacttatagc caaaagggaa gtatcgatac cattaggaac aatcggacat
     3961 tgattatcgg cacagaaatc cagtaaatcc cgttgatatt ggcttttaca gaaaattttg
     4021 tcatatctag ccaaaatttc cggggtatag gagtctgccg gcactttaat atcttgccaa
     4081 tcaaaaaaaa ttcgttctgc cttggtaccc ggacggcaat aggtgggatg acgagagatc
     4141 accaatgtct caaagtgatc ctgccaattc atgtggtaat aattaacata gcgaataccg
     4201 tcctgttccc cttccatacc ctcacaattg ccgaaaatcg tcactttata gccgcgtttc
     4261 gtccattcct gcgctaaaaa agccaccgat gcctgggtgc cactggcccc ccgttgccaa
     4321 atttccgccg gacgcatggg acgatatttt tctgtgtaaa taacgatcga ttttttcggc
     4381 caacgacggg cgctcaaacg ctgtttaatt tgaaataggg aaggtgcgct ggcccgtagc
     4441 cattgtttga gattagattg tagggatgga gtagcttgtg acactctttt tctccaagat
     4501 cgaggaaaaa taggggcgat gggtgataga gccttagttt ctcgaattta actcgatttc
     4561 tcgactcagt agaacaattc gagcctatca tataataggt tcaagtcaaa aatcaatctt
     4621 tattattgcc agctatctgc gaggaaatct atgaaaattg tcatcccgat cttattttat
     4681 agcaaaggag gagtagaaag agtcattatc tctttaatac ctagcttatt agaatacgtc
     4741 gaacaaatta ttatcgtcct ccctcgtaag gaaattgaat attttagata tttaatgcct
     4801 gattccgata aacttatcta tgaggatctt gctttttacc cccagagttt tgagacaaaa
     4861 ttaatctatt tttatgttct tctagcaaat ttttacaaaa ccctaagact gaatgctctt
     4921 catcgtcttt tctctcgtag aattgaactt ttacgcatcg aagcaagaat taatcagatt
     4981 gtcaagcgtt accaagctga tcactgtttg tatgggatga ctaatcggat ttctcccccc
     5041 aatgtcaagg ttactttaag tggcattatt tatgatgtct tttggcaatt tgctccctta
     5101 acttactcag aatcctatca agaaccctac aatgaagcgt taaaagaatg gttagatcgg
     5161 gccgatctac tgttcacaat ttcccaaaaa actaaagacg atgttttaaa agtttttccc
     5221 aatgctacct atgctagtaa gttaaaagct gtacccttag caggacttat cgagcagtct
     5281 aatcccaaga ttgatttagt caaaagcgag ctagttactt tttattttcc ctcctctttt
     5341 ggtatctaca aagatcatct gactttactg aaagcagcca ttacactagc gaaaaaaggt
     5401 ttaaaatttc aaatagtttt tatcggtaaa gaaaccgata atctagttaa tggaaatctg
     5461 caattatctc aacagtccca aactcaagaa taccaagatt atctcaaaga atgtaaccga
     5521 gtttatcggg aaaatcaaga aattttcgag agtcatatca agggattagg ttatcaagac
     5581 tatgaaacct tacaatttta ttatcaaacc tgttcctctg tggtatttcc ctcaaaatac
     5641 gaaggttttg gattagcaat agcggaagcc attttacaag gtattcccgt tatcgcttcc
     5701 gatttagaag tgtttcaaga acaggtagaa ttatataatt gtcccgaaag agtgcaattc
     5761 tatcctaggg gagatgcgga gagtttagcc aactgtttag aacaatttat cctcaatcca
     5821 attcctcgct tgttacccga agatatcccc aataaaatta atctctggac ttgggaagat
     5881 gtggcgaaaa aatatgtaga actactgaaa gaaaatgcag gttactgatc tctatatcta
     5941 cccgattaaa tcctgtcgag gtattcaatt atcccaagct gaagtgactc ctaaaggttt
     6001 actctgggat cgagaaatga tgttagtgga tggcaagggt aaatttatca ctcaaaggga
     6061 atatccgcaa ttagccaccg tttctgtcat gatgacagag gataattttt ctctgaaaac
     6121 atctcaagat tccctgactt tccagcctaa tttcacagga gaaaaaagag cggttcaaat
     6181 ctgggaatct cacaccatag ccatggatca aggggatgaa atcgccgaat ggttcgagaa
     6241 aattctcgac attccctgtc gtttagtgcg acaatctcct gattatccac gtcctgtgaa
     6301 tcctcgctat gcggggaatg atcatactcc cgtcagcttt gccgatggtt atccaatttt
     6361 attaactaat actgcttctt tagaggactt aaatcggcgt ttagtcacac cagtgccgat
     6421 gaatcgtttt cgtcctaata ttgttattag tagcgatcga gcttttagcg aaagtagttg
     6481 gcaaaagatc acaattggag agataaatta tgccctagtt aaaccctgta gtcgctgtat
     6541 tatcaccacc accgatcaag aaacaggtag gagaaatccc cagcaagaac ctttaaaaac
     6601 cttaagcact tttcgcagtt tccccggggg catcatgttc ggagaaaatg tcatccccga
     6661 aaagacaggt acaattaaag tcggcgatcc tattaccgta atctagagat atagagatat
     6721 agggggatag ggaaatgggg ttatcagtta tcagttatca gttatcagtt atcagatgtg
     6781 agttttcagt tcactgatta ctgttcactg ttcacagcaa aaaactcccc actccccact
     6841 gataactgtg cgggcatcaa ggttaagata aataaccata agaacaatac ttgactagag
     6901 acactgaaac catgcccctt gataccgcat cgattttaga agtattacgt cccgttcaag
     6961 atccagaact acaaaaaagc ctagtggaat tgaacatgat ccgcaatgtg gcgatcgatg
     7021 gcggaaaagt tagctttacc ctagttttga ccactcctgc ctgtcctttg cgcgaattta
     7081 tcgtggaaga ctgccaaaaa gctgttaaac agctgcccgg ggtggaaagt gtcgccgtgg
     7141 cagtaactgc cgaaaccccg caacaaaaag ctttacccga tcgccaaggt gtggaggggg
     7201 ttaaaaatat tatcgccgtt tccagtggta agggcggtgt gggaaaaagt accgtggctg
     7261 tcaatatcgc cgtcgccttg gctcatttag gggcaaaagt ggggctatta gacgcggaca
     7321 tctacggccc caacgccccg acaatgttgg gcttaaatga cgcacaagtg acggtacagg
     7381 gcgcaaatgg cgagatattg gaacccgctt ttaaccatgg catcaaaatg gtttccatgg
     7441 gctttttgat caaccccgat caaccggtaa tctggcgcgg tccgatgtta aacggcatta
     7501 tccgtcagtt tctctatcaa gtcaattggg gcgatttaga ctatttaatc gtcgatatgc
     7561 ctcccggtac cggggatgca caattaactt taattcaatc ggtcccccta gcgggtgcgg
     7621 tgattgtcac cactccccag acggtttctt taattgatgc gcgccggggt ttaaaaatgt
     7681 tccaacagct aggggcgcgg gttttgggta tcgtggaaaa tatgagttat tttatccctc
     7741 ccgatcagcc cgatcgctcc tatgatctat ttgggtccgg tggtggggaa aaaacctcgc
     7801 aagaattggg cattccttta ctgggatgtg tacctctgga gatatccctc cgggagggcg
     7861 gtgatactgg tgtaccggta gtcttggggc aaccagaatc ggcctcagct aaagccctga
     7921 tagcgatcgc tcgtcaggtg gcggcgaaag tatccgtggc agcctatagt tagggtttgc
     7981 ggcaaaaagt tttccctagg ggcagggtgt agggtgtagg gtgtagggtt ttacccattt
     8041 tcagggaaaa agtccctaaa ttttcccccc aatcactcca atgtccggtg cttttggatt
     8101 tcaaaaaggt ctaaaagtct tatcgaacaa tgtttttaga tttattcagc aaaccctagt
     8161 tagttagtaa atgtggggag agaaatggtg agaagacagt cctttattag taggaaacta
     8221 aggggtttta gtgagcggtt tagcttcatt ttccgggatc tagcccaaat ggatcggctt
     8281 ttgttcgttt tggtgacggg tttaaccgtt ttcggtggtt taatgattcg tagtgccgaa
     8341 agacatacca ctgctctcga ctggtggcaa cactggttat tcggggccgg gggagtggcg
     8401 atcgctttat ttctggcccg ttgtcgttac gaaagtctgc tgaaatggca ctggttaacc
     8461 tatcttctca ccaatctctc cctaattgca gtaattgtcc tgggggtgac ggcgaatggg
     8521 gcgcaaagtt ggattaatat cggcagtttt aacgtgcaac cgtcggaatt tgccaaggta
     8581 ggtttaatta tcaccctagc agccctccta catcatcgtc ccgccgataa tctttttgcg
     8641 atcgctcgtg tctttgtcgt cactgccatc ccctgggtgt taattatggc gcagccggac
     8701 ctggggacgg gattagtctt tggtgcaatt accctaggca tgatttactg ggctaatgcg
     8761 aaactgcctt ggatgattat cctcttatct cccctcgcat cggtttttct gtttaattta
     8821 ctttttcctg cttggattgt tttagcaatt atcatcgctg tgcttgcttg gtttaccctt
     8881 ccctaccgtt ttttatctac tttcatcgtg gtggcaacta atctggcagt gggtaaatta
     8941 ggcgaggttt tttggggttt attgaaagaa tatcaaaaag atcgtttaac cctatttctt
     9001 cacccagaaa agaatccttt ggggggaggt tatcaattaa ttcaatcccg cattgccatc
     9061 ggatcgggag aattattggg acggggatta caccaaggca cccaaaccca attaaatttt
     9121 attcccgaac aacacaccga ttttatcttc accgtagtcg gggaagaatt cggttttgtc
     9181 ggcagtattc tagttttaat agctttctgg ttagtttgtt ggcgtttgtt agttattgct
     9241 aatactgcta aagaaaactt cggttcttta atagcgatcg gtgttctctc gatgatcgct
     9301 tttcaggcaa ttcttaatat tagcatgacc gtgggattag ccccgattac agggattcct
     9361 ctaccttggt taagttatgg acgttcatct ttattaacca attttattgc cttgggttta
     9421 gtggaatccg tggctaatta tcgcccccgt aagcgtttgt actaattagt cgtgcagcta
     9481 tcagttatca gttatcagat ttgagtttta agtgtggagt attaaataga agtttcctac
     9541 tatcttttta ctgattactg tttactgttt actgatcact gatcactgag ttgatcttgg
     9601 gtacgggaga gccaataacc gacattaaaa aactttcttt caccagaaaa ctctcgaatc
     9661 gaaggactac aatttaaatc atccaatcta ctaatctatt tatttttatc cacttgcctt
     9721 ttgcgtgagt gctttttgca tgagtgcctg tcctcataaa tagccgataa tcaaaggatt
     9781 tagtatgatt tagcggaaat ttttcttaaa tttttcttaa atgcttgaca ttggccggat
     9841 tagtgcttat gatgttttcc atcacccaaa caaaggcaaa ttatttgcac aatcccgatt
     9901 ctgaaagcca tgcacagcaa agctttcagg gtttattgca ggtggttgtt tttatctgag
     9961 tgattaaacc tattcacttg aaattaccag aggaataaca atagcatatc ccaacgatca
    10021 acaaggtaaa gcacttcctt tctttgctcg tttcttgtcc gtaagcaaag aggaatcttc
    10081 catcaagtct ccttcccctg agcgtgaata taatgttaca ctcaaatacc cttctgactg
    10141 ggaagagttt taaactcagg ttacttgagt aattgcagca cttttcttga ttacaccgta
    10201 cagacaaaaa gctaaaagct ccactaagca aggctcgcag ccaaaaaaac tgcacgttga
    10261 gatactgaga agtgctgtca actaaattta gtaattactc aagaaaaaaa ccaatactgc
    10321 aataagtcaa gcaaaagcaa gactttttgc agcattttct tgagattagg atcaataaag
    10381 accgacccaa aagccttaag atgggttaaa atgtaaggac ttgcaactga tatgaaagaa
    10441 tcggctaaag ttgttttatt gttgacccat agcggcgatt ttttcacgat tgaccgcgtg
    10501 gctgaggcga tagaaaaaaa aggagccaca ccttttcgct tagataccga taaatttccc
    10561 ttagaggttc aattagcagc ccaatttaat ggtaaaaaaa gtttttatca attaacctat
    10621 aaccatcaat ctattgatag tcaacaggtg caatctgttt ggacaagacg tatttggcaa
    10681 cctgaaataa caggagattt agatcctcag tttcgggagg cttgtgtgcg agaatcccag
    10741 acgactttgg ctggtttttg ggatagttta aggtcagcgc gttggttaga taatttagcg
    10801 caaattgaga gggctaaaaa taagttatta caacttcgtt tggcctcaga agttggctta
    10861 attattcccc ccactttagt gaccaataat cctgatgctg ccagggagtt tttttcccag
    10921 gttcagggac gaatggtgag taaattgtta actgcgatcg cccgcagtat ggagtcgccg
    10981 gaattttttc tttataccag tagggtgaaa gcagaagacc ttgaggaagc ggaatcttta
    11041 cgctattgtc ctatggtttt tcaagcggaa attcccaaac aattagaatt gagaattgtc
    11101 gtggttaatg gacaaacatt tgtgggagct ttagaatctt ctcagtataa taattctgcc
    11161 gtagattggc gaagaccggg tattgatcct ggtgcttggc aacatcatac tttacccgat
    11221 tcactattgc agcaacttca gatttttatg gctaatatag ggttgaattt tggggcgttt
    11281 gattttatct taactcctgg gggagaatac gtttttttag aagttaatcc ctgtggtgag
    11341 tggggaatgt tagaacggga tttagattta cctatttctc atgcgatcgc tgatttttta
    11401 gtcttcggtt gatcggcaaa gctggcgctc aaaaaattgt taatattaag ttttcaagga
    11461 aatctatgac cgttttaatt gttactttta gccacgataa tgaaagtatt cctctggtaa
    11521 tcaaagccat agaaacgatg ggtaaaaaag ccttccgttt tgacaccgat cgcttcccta
    11581 cagaggtgaa agttgatctt tactcaggcg gtcaaaaagg cggaattatt accgatggag
    11641 aacaaaaatt agagctaaaa gaagtttctt ctgtctggta tcgacgcatg agatacggac
    11701 taaaattacc cgatgggatg gatagtcaat ttcgcgaagc ttctcttaag gaatgtcggt
    11761 taagtattcg aggaatgatt gctagtttat ctggttttca tcttgaccca attgctaagg
    11821 tagatcatgc caatcataaa caattgcagt tacaagtagc gcgacaatta ggtttattaa
    11881 ttccggggac tttaacttct aataatcctg aagctgtcaa gcaatttgct caggagtttg
    11941 aagcgacggg aattgtgact aaaatgcttt ctcaatttgc tatttatgga gacaagcaag
    12001 aggaaatggt tgtttttacc agtcctgtta caaaggaaga cctagataat ttggaaggtt
    12061 tgcaattttg tcccatgact tttcaggaaa acattcctaa agctttggaa ttacgcatca
    12121 ctatcgtcgg tgaacaaata tttacggcgg cgattaattc ccaacaatta gacggagcta
    12181 tctacgattg gcgaaaagag ggaagagcgc tccatcaaca atggcaaccc tacgatttac
    12241 cgaaaactat tgaaaaacaa ctactagaat tagtgaaata tttcggtctt aattatggtg
    12301 caattgatat gattgtcaca ccagatgaac gttatatctt tttagaaatt aatcccgttg
    12361 gcgagttttt ctggctagaa ctttatcctc cttattttcc tatctcccag gcgatcgctg
    12421 aaatcctagt taactcataa accaacaaat acagagccag agtattttat cgcatcgacg
    12481 aggctctgac tccagatttc ccccccgact cctgacttga aagagggtgt agggtgtggg
    12541 gtgtggggtg tagggtgtgg gaagtgggga agtggggagt tttctcagtg aactgataac
    12601 tgataactga taactgatag tagcttgtta atcaacttac ccagcaaaaa cctgattctc
    12661 tagttctcct gagaggggga agtctgaata ctctgtctca ctttcaatct gtttgtcacc
    12721 ccattagcta tattctcacc cattttaagg tttttgggtc tttatcgtaa cgataattaa
    12781 aaccatcttt ggcagtaata attttaccta aactggcacg actccggacg ttacttaaag
    12841 aataatcagt tagagactgc atttgttgat gggacaaacc ttcggctatt tcagaaactc
    12901 cggcaatttc cgcatcatgc tcagttttaa cctcagacaa agtctctatt tttaccgccg
    12961 aaaaattttc tgattttttt gacatcatca ctaggttatt cacttttttg cgctgataat
    13021 cttctacagt tgacagctgc caatgggaat tttctgttaa ctctaagacc catcgatgat
    13081 aattaaataa actttcccga tgaccaacac taataaaagt ggtttcactt tcctgtaatt
    13141 gctgatataa gctggcttca ttggccaaat ctaaagcgct agttgcttca tctaagatag
    13201 taaaagtggg tttagtgatt aatagtcgag caaaagctaa tctttgttgt tctccgaggg
    13261 ataaaatctg ttcccaattg acttgtttat ctaaggtatt tttgtcagtt aataaatgtt
    13321 gcaggttgac tttttttaag atattttcta attcttcatc gctcacctga tcattggtat
    13381 ggggataaag taattgctgg cgtaaactgc caaggataat ataaggacgt tggggcaaaa
    13441 ataacatttt agctaaagga ggacgcacta aacggccgct acctgctttc cataaaccgg
    13501 cgattgctcg taacaaggaa ctttttcctc gaccactggg gccaataatc agtaggcttt
    13561 ctcccaaagg gacagaaacc gataaatttt ccacaattac cttttcataa tcgggagttt
    13621 gtagggtaaa attttcaaaa gctagacgat tatcttccag ggtgataata cgacttaagt
    13681 gtttcggctc ttcactggca acttttaagg catctgtcaa ggtagctaac cgttcaatat
    13741 aacttgaaac tttacccgat gttccccatt cagtaattaa ttgtcccaag gcattagaaa
    13801 aaaggaaaca gcataaactc gcttgactta tttccccata atcgatttca tttttaataa
    13861 acatcggggt caaaataaac attgaaaata cactaatcgc tgactgataa cctcgattaa
    13921 aaagattatt aaaccgttcc caatttatca gtttttccga gctttgaata acccgttcaa
    13981 aacggccttc aatgattttt tcttcctgct cttctccctg aaaaaaagca atggattcgg
    14041 cgtggttacg cacatgggtt aaagcataat tataatcagc tttattatta agctccgatt
    14101 gattaatctt gtttaattct tgagttagat aaattgctat taaatttcct gcgatcgtat
    14161 aaattattag ataaaccgca atttgccgag aaattgtcca aagaataatc gcaaaagtaa
    14221 tcatttgtaa ccctttttcc acgaaggtga ttaataacct tagggtcatg gtcgtaattg
    14281 gttcaatttc ttgagataat ctttgatcgg gattttttaa agctgaggta aaatcaatat
    14341 tataataagc gcgagcattg agatattttt taacggtttg tttggttaac cacttatacc
    14401 aatccaatgc cacttgacga cgaataaatt gagaaaaagc aaataacccc gttgttaata
    14461 taatcaacaa actggataac cacagcgtat tgagatattt agaaaggtct ctatcttcaa
    14521 taataatatc gagtacataa cgattccagt agctactaaa agcttctacc cccaccgttg
    14581 ccaagatact taaaaggatc aaagctgaca ttccccaaga aatgatcaca tctccaaagg
    14641 cacgtccatt taactccgtt ggataccaat agggttgagc aacaattttt aggctttccc
    14701 aaaattgacc aaatagtgaa aagggattat tggcggactg gtcggacaca acagggtttt
    14761 ccgttgcttg tttagtagta tattgaggca tctagatcca attggcagtt cttttactgt
    14821 tctcttgaca actcgctatc atattgtagc ggcagaatta cctcttgttg tcttttgata
    14881 acatttttat ctagaagatg aaagccagct atggccagaa agctctgact tgactaagaa
    14941 ctacttgacc cacttttgtt actgtggcag tctagatgct gtttactggt cactgaaaaa
    15001 cccccttctc tcaatctccg tcatcctcaa aatgattagc tagtaggaat ttttgactgg
    15061 tattcaactc ttgccaaagc tggttaattt tctcgtaagc ttccgtggag gtaagctttc
    15121 ccccactgtg taaaccagcg ataaaagaga ctctcagggc gaattcctgc aaattagcgt
    15181 taaaaactaa attttgtggc gtgaataaac ccctgtagct ggttttaggg tagagaaagt
    15241 tatctttaga attgaatttt tccattgtta ccgacccccc agtataggat atctacatta
    15301 ttatatattt aaacttttgt taacttatgc ttaacctaag aatttaaacc tgtctctaga
    15361 ttcttaagag agtagctgct gaacttgatt agctgccgct atcccagagg ttaaggccgt
    15421 ttctagggaa gaatttctcg acaaatctcc tccttgacac caatcaccac aggcaactag
    15481 aggtaaagga ctattaacac ttaaacaggg tacagctaaa ccctgacgga ctaaagcata
    15541 acgccaacgg tgcagctgag accaatcggg gagaggcaaa gaagcacgag agagcaaatc
    15601 tagcttaact gcttctaaat cctcgctatc gagatattta accgcaaaat ctccactact
    15661 atgcacaaca aagacataat caggggatga ttgccgttta ctgctatcaa gtcctaacca
    15721 agcgatatcg gtggagggag caactgcggg taaagaatcg ccataacccg ccattaccgt
    15781 taaacaggga tcataaacga tcgatcgcaa ttctggcata gtagttatgg gagagttttc
    15841 taacaataac gctgcttgcg gggcgggaat agctaggaca atcgccgaaa actctccccg
    15901 aatttgaccg ttattgttta aaacccattt ttcttgacga ttttccagac gagtaaccag
    15961 aaaatctcgt tcaatttcta aacctttagc taaaaatttc gccacgctat tgatgccaga
    16021 aggagcaaca tagctagaat caaaccaaga agtaactaga ttctctctca ggagattatc
    16081 aattaaagcc gccgtttttt ccccctgaac agtcaaaaaa ggcaagccat gatccacagc
    16141 catctcttga ttagctcggt taacccgtcg tgtggctaaa cgaccaccaa tgcctctaga
    16201 cttatcaaat agcttaacag tataacctcg atcgcacaac tgacgagcgc aggttaaccc
    16261 ggctaaaccc gcgccaatga tcccaacctt aatcgaaaaa tttgtcacta gccttcctaa
    16321 tcctgtgaac ccttgagtta atattagtag taatcagcac tagcgaaact gatttgatta
    16381 ttggggctga ggaggacaaa ccacattgga cgaactacgc acagctttgg agttagccac
    16441 cgaggaggaa ctacagcaaa tcactaacat cctcttttgc cgtcgtttta accctctcga
    16501 ttatctacga gcgccggacg caatcgccgt tcaaagtcaa gattgggata gttggttaga
    16561 tagcgtcgag gatcgttttc gttatttggc tgccgatggt gtgacagtat taaaaggtca
    16621 aaccgaaaaa gttagttatc gtcaaattct cgtccgtgtc tgtcatttcc tcaaggttcc
    16681 ctactcccaa aaaatgccga caacggaaat tgaagcggaa atctatcttc acctcgttaa
    16741 caaagcttgg aaacgtctcc ccccatcgga acaaaaatcc ctctcgattc aaattcaaaa
    16801 agcactcgcg gattcccaca ctccccaacc cttacccgtt cacctacaac acaatcccct
    16861 cgatatagtc ctcaaaggcg gcagcgtcat cgccattaat tctatcctga aaccgattct
    16921 gctcaaacat atcgccgggc agttcgctct ccattttgcc cgctatcaag gggcaaaaac
    16981 tgccctagtc cagggtgggg caattgctaa tcaattagcc ctacaaacgg ctaaacaggg
    17041 catgactacg gcggcggccc gctacggagc ggtcagaacc gtgttaagtt tggtaggacc
    17101 ggctctatgg ggttggttta ttgccgatct cggttggaaa gcgatcgcaa ctgactacgg
    17161 ccggattatc ccgactattt ttgccatcgc ccaaattcgt ctgacccgtg acgattgttg
    17221 gcaacctgca tagcttgaca tcctcaccgc cgtaaacgga cggtgattcc caaacctcac
    17281 gatttaggtt tctgcttctt tcccttgcgg ggttccgcac ctgccttaac agatttactc
    17341 tgttctggtc ttatggtcgc tctacagact gacaccgcaa gccctgcggc caaaatattt
    17401 ttactagcgt taacatctcg gtcatggtgt gtcccacagt ctggacaatc ccattctcga
    17461 acatttaacg gcatcttttc gacaatatac ccacaattac tgcaccgttt agaactggga
    17521 aaccatctat ctatttcgat gtaatttctg ccgtaccaac ggcatttata ggctaattgt
    17581 cgggtgattt ctccccaact aacgtcagat attgcctgag ataatttcgg gtttttgacc
    17641 atattcttga cggctaaatt ctcaaccaca atcgtttggt tttcacggac taattgagtg
    17701 gttagcttgt gtaaatggtc ttttctgtta tcagtaattt gggcgtgaat cttggctact
    17761 ttgattctcg ctttttcccg attttttgag cctttctgtt ttcgagaaag actttttggg
    17821 tcctttcgca gtctccgata atgcctttta aaatgcttga gattagatac cttgtctcca
    17881 tcgctggtaa ttactaggct actaattcct aagtcaattc caatggcttt atcggttggg
    17941 ggtaatggct ggattgttgg gtcatcaaat cttattgaaa tatgccaacg tccagaagga
    18001 tgtaatctga ctgttactgt gcttgggtca catccgtctg gtatttgtct tgaccatcga
    18061 ataggtaaag gttctgtgca tttggctaaa tagatttgtt tgtctttaaa tttaaaggct
    18121 gatttggtaa attcggcact tcctccctga tgttttttct taaagttggg atacttagta
    18181 cgaccagcaa agaaattagt aaaagctgtt tgtaaatgtc ttaacccttg ttgtaagggt
    18241 acacagctta cttcattgag aaagtctaat tcttcttgct ttttccaatc ggtcaacatt
    18301 gaagaagttt gagcgtagcc tactctttct tgcttttcgt accaagcttg tgttcgttcg
    18361 tggagagctt tgttgtaaac taatcttaca catcccaaag tgcgccgcaa tagcgactct
    18421 tgttcgggtg tggggtaaaa tcgaaacgaa taggcttttt ccatacctca cattttagca
    18481 tatatttcgt aaatgtgcta atatttaaca gtaaagccgt cgtagaacga cggggtttca
    18541 gacccaaatt ttcgatgaaa aagatttttt gtcaagggtg ggagtggaat ttaatacttt
    18601 tttcagtttt tattgccatg atgctgccgg agttagcggg tatcggcatt attatcgtct
    18661 taattagggt ttttaccggc aattttcggg caattatccg gtcacgtctt aatcaaggtc
    18721 tcggtatcgt cactctttgg ttgatgatta gtgccagtct ttccccaact ccgggggatg
    18781 cttggattgg tttaggaaat ttcctgcctt ttttcttctt ttttagcaat attcggcaat
    18841 taattaaaaa accctctcaa ttgcgtcaac tagcttgggc gatggttatt ccgtcggttt
    18901 gggtagtggt gatgggatgg ggacaaattt ttctcggttg gcaaaaaccg gaagcattaa
    18961 aagggatttt tacctggccc tacggggcgg ggggtgaacc tttggggcga atgtcttcgg
    19021 tttttatgta tgctaatctt ttgggagctt atttattaat aactctgatt ttagccctcg
    19081 gtttattaat tctaacttgg cggcagtggc accgacaaag aaataccctt ttaaatctca
    19141 aagtcgggtt tttaacttta accatattta tcgatagtgt agggctattt ttaagcaatt
    19201 ctcgcaatgc ttgggcctta gcctttttag ggggcctaat tttcgccctt tatctgggtt
    19261 ggaatgcctt agtcgccggt tttatcctct tggggggggt gatttccctc gcttcttggg
    19321 gaccaaatcc tttcagggat attgtgcgtt ttttggttcc tagggcgatt tgggcgcgat
    19381 tatccgatga aatgttccct gatcgccctc tagcgactct cagatcaact caatggcgtt
    19441 ttactctaga aatgacccaa gaaagacccc tattcggttg gggattacgc agttttacac
    19501 ctctctacga aaaaagtcag ggattgtggc taggtcatcc ccataattta tatttgatgt
    19561 taatggcaga aacgggaatt atcggcttaa ttttattgtc tgtttgggtg ggttggattt
    19621 atgcccaagg gttgcgttta tttatctttc tacggcaaca aaaagccggg ggtgagttaa
    19681 ttatctttac ctatctggta gcctttggtg cttgtgtttt atttaatctc gtcgatgtca
    19741 ccctctccga tgtcaagatt aatactattg tttggtttct cttggctgcc attgctggtg
    19801 tcagtcaaag gttaaattat gaattatgaa ttgggaagtg gggtgtgggg tgtggggtgt
    19861 agggtgtggg gtgtgggaat tggggaagtg gggaagtagg gtgtggggtg tggggtgtgg
    19921 ggtgtggggt gtgggaattg gggaagtggg gaagtgggga agtggggtgt ggggtgtggg
    19981 gtgtggggtg tggggtgtgg ggtgtgggaa ttggggaagt ggggaagtag ggtgtggggt
    20041 gtggggtgtg gggtgtgggg agaataaata aaataatctc ctgaataagg gtgcatccca
    20101 cacttgcaga aataccaaca atcagcaatt atcagtgact cttaaattag tac
//
