LOCUS       NZ_AP024565            20810 bp    DNA     circular CON 26-SEP-2024
DEFINITION  Microcystis aeruginosa strain NIES-88 chromosome, complete genome.
ACCESSION   NZ_AP024565
VERSION     NZ_AP024565.1
KEYWORDS    RefSeq.
SOURCE      Microcystis aeruginosa
  ORGANISM  Microcystis aeruginosa
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Chroococcales; Microcystaceae; Microcystis.
REFERENCE   1
  AUTHORS   Phan,C.-S., Matsuda,K., Balloo,N., Wakimoto,T. and Okino,T.
  TITLE     Argicyclamide A-C unveil enzymatic basis for guanidine
            bis-prenylation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5478552)
  AUTHORS   Matsuda,K. and Wakimoto,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2021) Contact:Kenichi Matsuda Hokkaido University,
            Graduate school of phrmaceutical sciences; Kita 12, Nishi 6,
            Kita-ku, Sapporo, Hokkaido 060-0812, Japan URL
            :https://www.pharm.hokudai.ac.jp/tennen/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 87x
            Sequencing Technology             :: DNBSEQ; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019704275.1-RS_2024_09_26
            Annotation Date                   :: 09/26/2024 11:16:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,602
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,071
            CDSs (with protein)               :: 5,071
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 110 of 479
            Pseudo Genes (incomplete)         :: 380 of 479
            Pseudo Genes (internal stop)      :: 101 of 479
            Pseudo Genes (multiple problems)  :: 91 of 479
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 14:54:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2008807
            Orig. end                         :: 2029617
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024565.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated at DFAST https://dfast.nig.ac.jp/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20810
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="6"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20810
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20810
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10810](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10810
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    699..714
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 24.13"
                     /tool="antismash"
     gene            complement(1608..2138)
                     /locus_tag="LC603172_RS09930"
                     /old_locus_tag="MAN88_19250"
     CDS             complement(1608..2138)
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002798117.1"
                     /locus_tag="LC603172_RS09930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19250"
                     /product="chemotaxis protein CheW"
                     /protein_id="WP_061433348.1"
                     /transl_table=11
                     /translation="MLNNADFFAGNAQNIAPDNQELQTPVGELYLRFYLPSQDEFALSA
                     VGICEVIQQPLDRITPIPNASPLLLGTINLRGQVIWVADLGQFLGDSSRLNTDRAEIPI
                     IALKEEETILGLAVERLGDMEWLDQDKLGSAASIPDQIALYVQGEWILGQESKKTLRLL
                     DHLAILRSARWAA"
     gene            complement(2230..2595)
                     /locus_tag="LC603172_RS09935"
                     /old_locus_tag="MAN88_19260"
     CDS             complement(2230..2595)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202346.1"
                     /locus_tag="LC603172_RS09935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19260"
                     /product="response regulator transcription factor"
                     /protein_id="WP_002798118.1"
                     /transl_table=11
                     /translation="MSEILLVEDSQTQRELICDLLRNSGIKVTIATDGVEALEHIQRAN
                     PDLVVLDIVMPRMNGYEVCRRLKSDPKTKNVPVVICSLKGEVFDRYWGMKIGADAYIVK
                     PFEPIEFIYTIKQLLRR"
     misc_feature    3353..3366
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.2"
                     /tool="antismash"
     gene            3975..5306
                     /locus_tag="LC603172_RS09940"
                     /old_locus_tag="MAN88_19270"
     CDS             3975..5306
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012266256.1"
                     /locus_tag="LC603172_RS09940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19270"
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="WP_061433347.1"
                     /transl_table=11
                     /translation="MYRCQQNLMSMDKHLKAILEFICAESHQLTNCGIYYARQLDLKTQ
                     KIIGKFDLDKEYKNNKHYQVLSSQAAQQILKSIAESLKSFKELNQKSRKGELTDKPHLP
                     KYIKKEGLFLVTYPKQALKWVDNQIRIPLGKTVKQWFGLDSFYLPTTSNLRFQEIKELR
                     ILPRNHCFYVEFVYESKPVEIEVNPDNVLGLDSGLNNWLTGVSSLGTSFVIDGRHLKSV
                     NRWYHKQVATLKEGKPQGFWSTQLAKITEKRNRKIRDAVNKAARLVINQCLEHQRNKDQ
                     KEEGNLGKKNHQSFVSIATAKLKKRMRQLCEQCGIKFIETEESYTSKASFLDNDSLPKF
                     VEKPEGWQPSGKRTKRGLYRTAQNQYINADANGAANIIRKVSTTLNFDLSVVSRGVLTR
                     PQKFQCWSAKKTRSPLGILRHLWVGEVKKTFIMEDNYSCYTLQK"
     gene            5285..>6433
                     /gene="hmpF"
                     /locus_tag="LC603172_RS09945"
                     /old_locus_tag="MAN88_19280"
                     /pseudo=""
     CDS             5285..>6433
                     /codon_start=1
                     /gene="hmpF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002783054.1"
                     /locus_tag="LC603172_RS09945"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19280"
                     /product="pilus motility taxis protein HmpF"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLYLAEVKKQTRGFIGGYKTELKLLACQHNDQTWSAIPGEDILIY
                     DETNSLGEGALLLVNLSNNRQLQGNPELAGAEMVRQLQKISRLMERSKKDQEKIEQWKQ
                     SLTYQSEILNRQKMEMETRIEQIEQIEAEFDYLERKRQELETLKQQLNEQQRHLEEGQI
                     NCNSYPNLSPEKQQFIRSLAERLANNHDRGNSPSQILHSTLESVQEQQTILNSCWQSLE
                     EQKKAVENRQVANDEMLDYLEQRARELQTSRNALETAQIQLQIQETVLNQKQELFERLN
                     LTLQTTDSTRQMLLRLLQGEELDFDGKIDLEALEKMPLEELESLVKNLQTDLKRWEIFV
                     NDEEEELTLQCQTVEELEARMATIDESERANLEEELAEEQEKK"
     gene            6533..7519
                     /locus_tag="LC603172_RS09950"
                     /old_locus_tag="MAN88_19290"
     CDS             6533..7519
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012266743.1"
                     /locus_tag="LC603172_RS09950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19290"
                     /product="IS30-like element ISMae39 family transposase"
                     /protein_id="WP_221275333.1"
                     /transl_table=11
                     /translation="MSHQHLNIEQRNLLYQLSQEGNLSQRQMAVWLGCHQSTISRELKR
                     NQSSLGCYLPDTAQAQSETRRKNAKQPFKNVSESALELVKEGLKDYHSPEQIAGRLKRA
                     SQESLSHETIYQMIYQNYHGCGEYQKYLRRGLCQRKSRGGAKSKRGGIPGRVDISERPA
                     IADQKTEIGHWESDTMIGGNHLGVLVTHVDKASKFLVAGLAKNKTASEINRVTEKLFQE
                     IHPDKRKTFTCDNGKEFCGHQELSQKLGADFYFATPYHSWERGLNEHTNGLLRQFFPKG
                     TNFKIVKPEQVEIAVNLINNRPRKCLDYRTPNEVFYKGRSDSDAIQT"
     gene            <7526..8050
                     /locus_tag="LC603172_RS09955"
                     /old_locus_tag="MAN88_19300"
                     /pseudo=""
     CDS             <7526..8050
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002783054.1"
                     /locus_tag="LC603172_RS09955"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19300"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="QGMLAATLVGQRRNLQERQAIRSQHLKILQRRQGILSPEDAQKCS
                     FEPLLILLEDSYQNNLQESQRLAAEIKGLQEDLPKNRDTIDEQWSDLEQKTREFEEQER
                     QWRDANLVLVRLKTQVQQYETFLQPVQDQLDQIRQKLETISQWLMPMERQFKNETHQPQ
                     ELTKKGSSGTV"
     gene            8080..9066
                     /locus_tag="LC603172_RS09960"
                     /old_locus_tag="MAN88_19310"
     CDS             8080..9066
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012266743.1"
                     /locus_tag="LC603172_RS09960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19310"
                     /product="IS30-like element ISMae39 family transposase"
                     /protein_id="WP_221275333.1"
                     /transl_table=11
                     /translation="MSHQHLNIEQRNLLYQLSQEGNLSQRQMAVWLGCHQSTISRELKR
                     NQSSLGCYLPDTAQAQSETRRKNAKQPFKNVSESALELVKEGLKDYHSPEQIAGRLKRA
                     SQESLSHETIYQMIYQNYHGCGEYQKYLRRGLCQRKSRGGAKSKRGGIPGRVDISERPA
                     IADQKTEIGHWESDTMIGGNHLGVLVTHVDKASKFLVAGLAKNKTASEINRVTEKLFQE
                     IHPDKRKTFTCDNGKEFCGHQELSQKLGADFYFATPYHSWERGLNEHTNGLLRQFFPKG
                     TNFKIVKPEQVEIAVNLINNRPRKCLDYRTPNEVFYKGRSDSDAIQT"
     gene            9252..9380
                     /locus_tag="LC603172_RS27975"
                     /old_locus_tag="MAN88_19320"
     CDS             9252..9380
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS27975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MAN88_19320"
                     /product="hypothetical protein"
                     /protein_id="WP_260324550.1"
                     /transl_table=11
                     /translation="MWYRLRLVIYTQIVMRQSDREIIILAAVASLLAVVAEVAIAF"
     gene            9507..9731
                     /locus_tag="LC603172_RS09965"
                     /old_locus_tag="MAN88_19330"
     CDS             9507..9731
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002738436.1"
                     /locus_tag="LC603172_RS09965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19330"
                     /product="hypothetical protein"
                     /protein_id="WP_061433346.1"
                     /transl_table=11
                     /translation="MNISVTGQVKRIEIGTGTWALIADNGQTYELMEPPAELTQKKAKV
                     TVIGQIRSDVMTLAMIGPVLQVISFQSLP"
     gene            10001..10810
                     /locus_tag="LC603172_RS09970"
                     /old_locus_tag="MAN88_19340"
     CDS             10001..10810
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002757608.1"
                     /locus_tag="LC603172_RS09970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19340"
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="WP_002798124.1"
                     /sec_met_domain="PT_phytoene_like (E-value: 5.8e-24,
                     bitscore: 83.2, seeds: 61, tool: rule-based-clusters)"
                     /sec_met_domain="phytoene_synt (E-value: 9.6e-08, bitscore:
                     29.9, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLRDNALTILQETSRTFYIPISRLPDRLLEAVASAYLCMRAIDE
                     VEDHPDLDNFSKSQILRQISLNLQAGTENSTKEDFAFGLVHREILPEVTLRIGEWALLA
                     PDSIAPRVWDATAAMADRMAYWAGNNWDIRTEIELDQYTFSVAGAVGLLLSDLWNWYDG
                     TQTNRSYAIGFGRGLQAVNIARNHGEDLRRGVSFLPQGWTISDIHNYARRNLKLADYYT
                     QSLPNGPALDFCKIPLALAHGTLEVLALGKEKLSRSDVIALVQQITR"
     gene            complement(11117..11599)
                     /gene="petD"
                     /locus_tag="LC603172_RS09975"
                     /old_locus_tag="MAN88_19350"
     CDS             complement(11117..11599)
                     /GO_component="GO:0042651 - thylakoid membrane [Evidence
                     IEA]"
                     /GO_function="GO:0045156 - electron transporter,
                     transferring electrons within the cyclic electron transport
                     pathway of photosynthesis activity [Evidence IEA]"
                     /GO_process="GO:0009767 - photosynthetic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /gene="petD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010873379.1"
                     /locus_tag="LC603172_RS09975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19350"
                     /product="cytochrome b6-f complex subunit IV"
                     /protein_id="WP_004267928.1"
                     /transl_table=11
                     /translation="MSTLKKPDLSDPALRAKLAKGMGHNYYGEPAWPNDLLYVFPVVIF
                     GTIGLCTGLAIMDPTMIGEPADPFATPLEILPEWYLYPVFQILRILPNKLLGIACMAGV
                     PLGLMLVPFIESVNKFQNPFRRPVATAIFLFGTVVTIWLGIGATFPIDTSLTLGLF"
     gene            complement(11651..12319)
                     /gene="petB"
                     /locus_tag="LC603172_RS09980"
                     /old_locus_tag="MAN88_19360"
     CDS             complement(11651..12319)
                     /codon_start=1
                     /gene="petB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012306470.1"
                     /locus_tag="LC603172_RS09980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19360"
                     /product="cytochrome b6"
                     /protein_id="WP_002798126.1"
                     /transl_table=11
                     /translation="MFTKQVTDSKAYQWFQERLEIQGIADDISTKYVPPHVNIFYCLGG
                     ITLVCFVIQFATGFAMTFYYKPTVAEALSSVQYIMNEVNFGWLIRSIHRWSASMMVLMM
                     ILHVFRVYLTGGFKKPRELTWVTGVVLAVITVTFGVTGYSLPWDQVGYWAVKIVSGVPA
                     AIPVVGDQLVTLMRGSESVGQATLTRFYSLHTFVLPWSIAVFMLLHFLMIRKQGISGPL
                     "
     misc_feature    complement(12503..12522)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 21.83"
                     /tool="antismash"
     gene            12602..13081
                     /locus_tag="LC603172_RS29170"
     CDS             12602..13081
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF033518.0"
                     /locus_tag="LC603172_RS29170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="IS607 family transposase"
                     /protein_id="WP_199308304.1"
                     /transl_table=11
                     /translation="MTLRKAVELWGLHPNTLREYADEGKIKSIKNEAGQRLYDVQSYVN
                     GAARASLVCYCRVSSIKQRDDLERQVSFMRGFYPEAEIIKDIGSGLNFQRKGLRAILDR
                     LLWGNKLTIVMSTLDRLCRFRFELIQYMVEQNGGKILVLDQTVHSPESELTADLL"
     gene            13011..14246
                     /locus_tag="LC603172_RS09990"
                     /old_locus_tag="MAN88_19370"
     CDS             13011..14246
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1192: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /locus_tag="LC603172_RS09990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19370"
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="WP_204377852.1"
                     /transl_table=11
                     /translation="MAKSWFSTKLFTVPNQNLQQTCYKYFTSSLAECPDSGSTLKKSKK
                     VRIYPTTEQKKLMKHWWGVSRFVFNQTIKYLQEPDRKANWKAIKTGIINDLPEWAKEVP
                     YQIKSIAVKDACEAVKNAQRKYQKTGEIQKIKFRSRKDKQQSCYIPKSAISEKGIYHTK
                     LGQIRFTESLPDNFGDSRLVSRNGQYYLAIPHEVQRCESDNQGRIVALDPGVRDFLALF
                     SPNSFGSIGSQDIGKIQRLCYHLDDLISRSTKVNAKTRNRYRKAASRLRNKIRNLVDEL
                     HHQVARFLVDNFDVILLPTFEVSEMVLKNSRKIRSKSVRQMLNWSHYRFKQFLKHKAFE
                     MGKLVIDCCEAYTSKTVSWTGELIHNLGGKKVIKSKIDGKTMDRDINGARGIFLRALGD
                     TPSLRDSLRVHC"
     gene            14288..15724
                     /locus_tag="LC603172_RS09995"
                     /old_locus_tag="MAN88_19380"
     CDS             14288..15724
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016516520.1"
                     /locus_tag="LC603172_RS09995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19380"
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="WP_061433367.1"
                     /transl_table=11
                     /translation="MLLNTYDPEGFYDELFLDDGQPRPHASPLIKWLQNLPPRELQQHR
                     ETAQSALFELGVTFNVYSDNQGVEKIFPFDIIPRIVSAEDWDYLERGLKQRIKALNLFL
                     SDIYNDQLIIKDGIIPPELIASATGFLKPCVGLKPAGGIWCHITGTDLVRDKDGQWYVL
                     EDNLRVPSGISYVLENRRVMKSTFPDIFQTMNVKPVDDYPSHLLETLLNLAPPQLPNPT
                     VVVLTPGTYNSAYFEHSFIAQQMGVELVEGRDLVVFDGYLQMKTTKGLRRVDVVYRRLD
                     DDFLDPAVFRPDSMLGVPGLLKVFQEGRVALANAPGTGVADDKVIYAFVPDMIRYYLGE
                     EAILNNVPTYLCWREKDLDYVLNNLDKLVVKAANESGGYGMLVGSQATPEQRAEFAPRI
                     AANPRNYIAQPVLSLSRVPTLIDTEVEGRHVDLRPYILHRGDEIYVHPGGLTRVALKKG
                     SLVVNSSQGGGSKDTWVLTK"
     gene            15839..16840
                     /locus_tag="LC603172_RS10000"
                     /old_locus_tag="MAN88_19390"
     CDS             15839..16840
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002785396.1"
                     /locus_tag="LC603172_RS10000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19390"
                     /product="alpha-E domain-containing protein"
                     /protein_id="WP_061433344.1"
                     /transl_table=11
                     /translation="MLSRVADSIYWLNRYIERAENVARFMDVNLNLMLDVPTSVLQQWE
                     PLVLTTGDLEQFKKRYGTATAENVINFLTFDTEYSSSIISCLRWARENARSVREIISSE
                     MWEQVNGFFLMVKDASSYHPQTTLPNFFTQVKLASHRFAGVMDATMTHNEGWHFGVMGR
                     LQERADKTARILDVKYYYLLPSAQWVGTSLDQIQWIALLRSVSAYEMYRKCQRRITPSD
                     VAEFLILNREFPRSIHFCLRELDHCLHQVTGTPVGTWGNSVERSLGRLCAEMSYLTIED
                     IIEMGLHEFLDHIQKRVNDVGIKMTETFFVFNPELASPLPVNNFQYQSQLIT"
     gene            16840..17706
                     /locus_tag="LC603172_RS10005"
                     /old_locus_tag="MAN88_19400"
     CDS             16840..17706
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012266263.1"
                     /locus_tag="LC603172_RS10005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19400"
                     /product="transglutaminase family protein"
                     /protein_id="WP_061433343.1"
                     /transl_table=11
                     /translation="MRYHIRHQTNYFYNQPVILSPHLLRLRPKSDGGQTLREFTLKINP
                     EPLTITNIIDLDGNSCLQIWFNQPTEELLIDVVSEVETNGVNPFQYLLEPWATCLPFDY
                     PISLHSQLQPYRQFYGLANDPDVTALAQEIMQETEGQVLSFLFNLNDKIYRNCQQIIRL
                     TGEPYLPGNTWKNKQGSCRDFVVLFMDVCRCVGLASRFVSGYQEGDLDQEERHLHAWVE
                     VYLPGGGWKGYDPTHGLAVSDRHISLVSSPIPRYCAPVEGNARPVSLGHQVIESQLSST
                     ISIDLLS"
     gene            17841..18038
                     /locus_tag="LC603172_RS27980"
                     /old_locus_tag="MAN88_19410"
     CDS             17841..18038
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS27980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MAN88_19410"
                     /product="hypothetical protein"
                     /protein_id="WP_239698227.1"
                     /transl_table=11
                     /translation="MARVWTKKNAAREAIIKYADFLIEQLEETPQVNIQIRSLKRLANG
                     KLVTEVLEELTFEQNSASSR"
     gene            complement(18094..18399)
                     /locus_tag="LC603172_RS10015"
                     /old_locus_tag="MAN88_19420"
     CDS             complement(18094..18399)
                     /GO_component="GO:0032993 - protein-DNA complex [Evidence
                     IEA]"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002794782.1"
                     /locus_tag="LC603172_RS10015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19420"
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_239698226.1"
                     /transl_table=11
                     /translation="MTVIVMYNVMYIIKLAMVSKIRKQIYIEAEQNNLLKEKARQTGLS
                     EAEIIRQAIDQHIISVKSPTPNLSAWEREKAFITGLENRPSQPGKRDWRREDLYER"
     misc_feature    complement(18657..18672)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 21.37"
                     /tool="antismash"
     gene            18763..19200
                     /locus_tag="LC603172_RS10020"
                     /old_locus_tag="MAN88_19430"
     CDS             18763..19200
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002785401.1"
                     /locus_tag="LC603172_RS10020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_19430"
                     /product="DUF29 domain-containing protein"
                     /protein_id="WP_061433342.1"
                     /transl_table=11
                     /translation="MSSTAYETDYNQWLKETVKQLQERNFEQVDWDNLIAEIESMGKSD
                     RRALMSLLTRLIEHLLKLAYWQEEKKRSGNHWAAEIVNFRAQIQHRLEDSPSLRPELAA
                     MYDKVYPVAIKSVSQLFSLNSDAHISLEQTLDDNWFPPAEK"
     misc_feature    complement(18812..18828)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.01"
                     /tool="antismash"
     misc_feature    complement(20360..20375)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.61"
                     /tool="antismash"
     misc_feature    20370..20388
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 23.79"
                     /tool="antismash"
ORIGIN
        1 ctcgaattgg ctttgcagat cgttgagact gcgcttaatc tgactaatta gcaactttaa
       61 acctagccaa gtcagggcca tggttcccag tccggttaat aacatcatgg ttagtaccgg
      121 ttgactgaag ggggaaagac tggctgaggc tggcggtttc ggggccggtt tgggggcaac
      181 cttttcggtt tttgcctggc tattcggggc tggtttggca gccaagagcg tagaaagcca
      241 agaggtggta ctaacggcaa aaattatgat cacaggggta atacccgcta aagccgctag
      301 gattagttgt tttttgccta aagaagcgtt gacaaaaaag cctaatttgc ccggattgac
      361 ttccaccact ggcttgagca agcactcgga ggcatgggta agatgactat ctcttttgtc
      421 attgggggtt tttagacgag aaggattaaa actaggctct cccacaccag aactggcatt
      481 aaggcgcgca ctgggttcgg ggagaaaacc accacttaaa gcttcccccc ccgaaggggt
      541 aaatagctcc cagtccccca gtccttgaac cgtatccgtc agagctagtg gggaaaaacc
      601 gtctaaatcc tcaccattga gttctggttg cccaaaatct ccggtttcgt cttcctcgtc
      661 tcttttttga gtgactctgc cctgattagg gccagttttt gccccttgtg ggtaggtcga
      721 ttccgggtca atggcaaaag tgctttcttc tagggtatcc ctataggata actcctgatc
      781 tctaccggtg aggggatttc cccccagagc agaggaaacc ataaaagtgg cttctcccgt
      841 tgaccctaac tcaccgtccc acccttcctc cagcgagtca atctccgccg cttctaaaaa
      901 ggggaaaggg gcttccccct ccgccgaatc cctagaccac tgggaaccgt gagagccagg
      961 gcccggcagg gtaagggaac taaaggcggt ttcctccgat tcctcaaaat catcgtcata
     1021 ggaatggact ttacttaccg taggctcgct gggatcctcc tcattgaaaa gactactatt
     1081 ccaatccagc cctttttttt gtccatcggg gtcagcccct ggcgaattcc aggagcgaga
     1141 atggttaaaa ccttgaccta gatcgaaagc tgcctcgctg taggcaatgc tttccgtagc
     1201 agcgatcgta aaatctccct cctcggcttc aaaacgcaat tgccgaatct gatcgagacc
     1261 acgctgggca taatcgacta gatcggagct attgggagaa tttttagcca attgcagcac
     1321 tttttcgtaa cgttcctccg ccaatctgta ttgttgtagt cctagatgaa tgtgaccctg
     1381 taaaagcatg acattggcat cctcgggata cttgctgatc atctctttga caatgacggc
     1441 ggcatctgag aggttgccct ggcaataggc ggtgttagcc tttctataaa gttttgcgta
     1501 ttctttccct gaaggcattt gcccctcccc tagtaaagtt aaatgaagta gttagtagtt
     1561 tcaagaaaga attcctataa atcctattct gctatttttt tcaggtttta agccgcccaa
     1621 cgggcagaac ggagtatggc cagatgatcg agtagtctta aggttttttt tgattcttgg
     1681 cccagaatcc actctccctg cacatagagg gcgatctgat cgggaatact ggccgcacta
     1741 cctagtttat cttggtcgag ccattccata tctcccaaac gttccaccgc taaaccgagg
     1801 atcgtttcct cttctttgag cgcaattatg ggaatttcgg ctcgatcggt gtttaagcgg
     1861 ctgctatccc ccaaaaattg cccgagatcc gccacccaga tcacttgacc ccggagattg
     1921 atcgtgccga ggagcaaggg tgaagcattg ggaatcgggg taatgcgatc gagtggttgc
     1981 tgtattacct cgcaaattcc cacggcacta agggcaaact cgtcctgaga gggtagataa
     2041 aatcgcaagt acagttcacc gacgggagtt tgtaattcct gattgtcggg ggcgatattt
     2101 tgagcgttac cagcaaaaaa atcggcgttg ttaagcatga gtttagttct cttgcaacta
     2161 ataattcggg cggcgttttt agctctcttg acctatgtca tcgctagact gtccccggtg
     2221 agggcgattt tatcggcgca acagttgttt aatggtataa atgaactcaa tcggttcaaa
     2281 gggcttgaca atgtaggcat cggctccgat tttcatgccc caatagcgat caaagacttc
     2341 ccctttcaag gaacaaatga ccactgggac atttttggtt tttggatccg acttgagacg
     2401 acgacacacc tcataaccat tcatgcgggg catgacgata tcgaggacga cgagatccgg
     2461 attggctctc tgaatatgtt cgagggcttc caccccatcc gtggcgatcg tgaccttaat
     2521 gccgctattg cggagtagat cacagatcag ttccctttgc gtttggctgt cttctaccag
     2581 caatatttcg ctcataactc cctacctagc taggctgcca agaacaacaa tggcaaaaga
     2641 tgcccgataa tagtccccgt tgaccggatg ctcggaaatt tctctcattt ttgcctccaa
     2701 cgcaatctga aatttatcag gggtttctct ctacacaagg acttgattca ccctttcggg
     2761 tttcatctct atagttccca attccaccgt taaaaaccac cggccaagag attttttttc
     2821 gggtttgggg gttgggtgtg aggttttggg gtgttgggat ttaggatttt aggcttttag
     2881 ttgaaattcc tcagtacaat actgcgaatc tcgatctcat tgcagcttcc ttccataatt
     2941 tctcggaatc tcccccaata gaccctattt cttttagtct aggacaagca tttatcttac
     3001 ttgtgaggta caaagtatcg ggttttgggg aaggctggaa ctggggaagg ctgcagcagt
     3061 agctggtcgt ctggggatga tgccaaatgc cgtcatactt catcttgatg agaaacgctc
     3121 tcttacacca aatccgtttt taatagtgtg attaactctc aagttatcaa ttgtttctcc
     3181 ctgctcccca ctccgcagct ccagcgctcc cttctcccca caccccgctc tcctctttta
     3241 aacaggattt agtattacct cctcttgtca ctaataataa atctgataag ctaattatgt
     3301 aacatatttt tcagtaagct tgactgaatt taaaccagcc ctagagattt ttagtacaaa
     3361 agttctaact tcctatcttt gcttgtagct ctcatgccgt cttaaccgat aatttagatg
     3421 tgtagtcagc ttattcacag cagccagagg taaaaagggt cagaaaacac gagccgattt
     3481 atgagattga ctgccgaatt ttttgagact aggacagtac gtttgcgctg aaaaatctcc
     3541 aaaaattttc cagtggggaa gagggaaaat tgagaacaaa tgtatgaatc aaaaagaaaa
     3601 actcgaatga tagaaattaa aaaatttttg ggatcggatg gttacaccga aaccacagac
     3661 tggccgctcc tagcctcgat cgccaactct ctctaaaacc gattaccgaa ataacacggg
     3721 agcatgaaag ccattctctt tttaagcaat ggatgaaatg cgacccgacg ggttttaacc
     3781 cttcgtatcc ttttcctcta attttagact atcaatccag tcttcgatga gtgctgttat
     3841 ggttttgtct ttctgggcag catatcaata tagcttattt cttctgcggt caaaacaaat
     3901 tgttaatgat ttagtcttca tacatatata attagtgcat ttatgttagt ataacatcag
     3961 gaggtaaaaa tacaatgtat agatgccagc aaaatctaat gagcatggat aaacatttaa
     4021 aggctatcct tgagttcatt tgtgctgagt ctcatcaact gactaactgt ggaatttatt
     4081 atgcccgtca acttgatttg aaaacccaga aaattattgg caaatttgat ttagataaag
     4141 agtataaaaa caacaaacac tatcaagttt tatcctctca ggcagcacag caaatactta
     4201 aaagtattgc tgagtccttg aaatcattta aagagctaaa ccagaaatct agaaagggag
     4261 agttaacaga taagcctcat ttacctaaat acataaaaaa agaaggtctc ttccttgtca
     4321 cttatcccaa gcaagcccta aaatgggttg ataatcaaat taggatccct ttaggaaaaa
     4381 ctgttaagca atggtttgga cttgatagct tctatttgcc aacgacctcc aatttgaggt
     4441 ttcaagaaat taaggaatta agaatactgc ccagaaatca ttgtttttat gttgagtttg
     4501 tttatgagag taaaccagtc gagattgagg tcaatcccga taatgtgcta ggtttagatt
     4561 caggtttaaa caattggcta acaggagttt ctagtcttgg tactagcttt gtaattgatg
     4621 gaaggcatct taagtctgtt aatcgttggt accacaagca ggtagctact ctcaaagaag
     4681 gaaaacctca aggattttgg tcaactcaac tcgctaaaat cacagaaaag cgtaatcgaa
     4741 aaataagaga tgctgttaat aaagcagcta gacttgttat taatcaatgt cttgagcatc
     4801 aaaggaacaa agaccaaaag gaggaaggta atttaggcaa aaagaaccat caatcttttg
     4861 tttcaattgc gacagcaaaa ctcaaaaaac gcatgagaca attgtgtgaa cagtgtggga
     4921 ttaagtttat tgaaacagaa gaaagttata cctcaaaagc ttcattctta gacaatgact
     4981 cgctacctaa atttgttgaa aaacccgaag ggtggcaacc atcaggaaag agaacaaaaa
     5041 gaggattgta tcggactgct caaaatcagt atattaatgc tgatgctaat ggggcagcta
     5101 acataattcg gaaagtaagc acaacactta attttgattt aagtgttgtg tctaggggcg
     5161 ttttgactcg tccccagaaa ttccagtgct ggtcagctaa aaagacgcga agtccgctgg
     5221 gaatcctccg tcatttatgg gtcggagaag tcaaaaaaac ttttattatg gaggacaact
     5281 actcgtgcta taccttgcag aagtaaaaaa acagactaga ggttttatcg gtggttacaa
     5341 aaccgaattg aaactattgg cctgtcagca taatgatcag acgtggagtg ccataccggg
     5401 ggaagatatc ttaatctacg acgaaactaa ctccctgggt gaaggtgcgc ttctcttggt
     5461 aaatttgagc aataatcgtc aactgcaagg aaacccggaa ttagcgggag cggagatggt
     5521 gcggcaactg caaaagattt cccgtttgat ggagcgcagc aagaaagatc aagagaaaat
     5581 cgagcagtgg aaacaatccc tcacctatca aagcgagatt ctcaaccgcc agaaaatgga
     5641 aatggagaca agaatcgagc aaattgaaca aatagaggca gaattcgatt atttagagcg
     5701 caaaagacaa gaactggaaa ccctaaaaca acaattaaac gagcaacagc gacacctgga
     5761 agaaggacaa ataaattgta actcctaccc caatctttcc ccagaaaaac aacaatttat
     5821 tcgctctcta gcggagcgtt tagctaataa tcatgatcgg ggcaattctc cctcgcaaat
     5881 tctccattcc actcttgaat ctgtgcagga acaacagaca attttaaata gttgttggca
     5941 gagtttagag gagcagaaaa aagctgtaga aaatcgtcag gtcgccaacg atgaaatgct
     6001 cgattatctc gaacaaagag ctagggaatt acagacgagt cgcaatgctt tggaaaccgc
     6061 tcaaatacag ttacaaatac aagaaactgt tctgaatcaa aaacaggaat tattcgagcg
     6121 ccttaacctc accctacaaa ccactgattc tactcgacaa atgctccttc gtctcctgca
     6181 aggggaagaa ctagattttg atggtaaaat cgatctagaa gctcttgaaa aaatgccttt
     6241 agaagaattg gagagcttag ttaagaactt acaaacagac ctcaagcgct gggaaatttt
     6301 tgtcaacgac gaagaggaag aattaactct ccaatgccaa accgtggaag aattagaagc
     6361 aagaatggcc acaatagatg aatccgagcg agctaattta gaagaagaat tagccgagga
     6421 acaggaaaaa aaagcgccaa ttcaaaaatg aaacgcatca acctcaagag ttgacaaaga
     6481 aaggctcatc aggtactgtc tgatcattaa caaaaacaaa tcagacccaa aaatgagcca
     6541 ccaacatctt aacatagaac aaagaaactt gctctaccaa cttagtcagg agggaaattt
     6601 atctcaacgg caaatggccg tctggctcgg atgtcatcaa agcacaatat cacgggaatt
     6661 aaaaaggaat caaagttccc ttggctgcta tctacctgac accgcacaag ctcaaagcga
     6721 gacaaggcga aaaaatgcca aacaaccctt taaaaatgtc agcgagtcag ccttagagtt
     6781 ggtcaaagaa ggattgaaag attatcatag tcccgaacaa atagcaggtc gtctgaaaag
     6841 agccagtcaa gaatctctca gtcacgaaac catctatcaa atgatctatc agaactatca
     6901 cggatgtgga gagtaccaga aatatttacg ccgggggctt tgtcaaagaa agagtcgagg
     6961 cggagcaaaa agtaagcgtg gagggattcc agggcgtgta gatatcagcg agcgaccagc
     7021 aattgctgac cagaaaaccg agatcggtca ttgggaaagt gacacgatga ttggaggaaa
     7081 tcatctagga gttctcgtta cccatgtaga caaggcctcg aaatttctag tagcaggatt
     7141 agccaaaaac aaaaccgcat cggaaatcaa tagagtcaca gaaaaacttt ttcaagagat
     7201 tcaccctgac aaaagaaaaa cctttacttg tgacaacggc aaagaatttt gtggacatca
     7261 agagttgagt cagaaattgg gagcagattt ttattttgct actccttatc attcttggga
     7321 gaggggatta aacgagcata ctaatggact attaagacaa ttttttccca agggaacgaa
     7381 ttttaaaatt gttaagccag agcaggtgga aatagccgta aacttgatta acaatcgtcc
     7441 tcgaaaatgt cttgactatc gtaccccgaa tgaggtattc tataaaggta gatcagacag
     7501 tgatgcaatt cagacttgaa ttggccaagg aatgctggcg gcaactttgg tcggacaaag
     7561 acgtaatctc caggaaaggc aagctattcg ctctcaacat ctgaaaattc tgcaacggag
     7621 acaggggatt ttatccccag aagatgccca aaaatgtagc tttgaaccat tgttaattct
     7681 cttggaggat agttatcaaa ataatctcca agaaagtcaa cgtttagcag cagaaatcaa
     7741 gggactacaa gaggatttac caaaaaacag agatactatt gatgagcaat ggtcggattt
     7801 agagcaaaaa actcgtgaat ttgaagaaca ggagcgacaa tggcgcgatg ctaatctggt
     7861 attagtgcgt ttaaaaactc aggtacaaca gtacgaaact ttcctgcaac ccgtgcagga
     7921 tcaactcgat caaatccgtc aaaaactaga aactatttcc cagtggctga tgcctatgga
     7981 acgccaattc aaaaatgaaa cgcatcaacc tcaagagttg acaaagaaag gctcatcagg
     8041 tactgtctga tcattaacaa aaacaaatca gacccaaaaa tgagccacca acatcttaac
     8101 atagaacaaa gaaacttgct ctaccaactt agtcaggagg gaaatttatc tcaacggcaa
     8161 atggccgtct ggctcggatg tcatcaaagc acaatatcac gggaattaaa aaggaatcaa
     8221 agttcccttg gctgctatct acctgacaca gcacaagctc aaagcgagac aaggcgaaaa
     8281 aatgccaaac aaccctttaa aaatgtcagc gagtcagcct tagagttggt caaagaagga
     8341 ttgaaagatt atcatagtcc cgaacaaata gcaggtcgtc tgaaaagagc cagtcaagaa
     8401 tctctcagtc acgaaaccat ctatcaaatg atctatcaga actatcacgg atgtggagag
     8461 taccagaaat atttacgccg ggggctttgt caaagaaaga gtcgaggcgg agcaaaaagt
     8521 aagcgtggag ggattccagg gcgtgtagat atcagcgagc gaccagcaat tgctgaccag
     8581 aaaaccgaga tcggtcattg ggaaagtgac acgatgattg gaggaaatca tctaggagtt
     8641 ctcgttaccc atgtagacaa ggcctcgaaa tttctagtag caggattagc caaaaacaaa
     8701 accgcatcgg aaatcaatag agtcacagaa aaactttttc aagagattca ccctgacaaa
     8761 agaaaaacct ttacttgtga caacggcaaa gaattttgtg gacatcaaga gttgagtcag
     8821 aaattgggag cagattttta ttttgctact ccttatcatt cttgggagag gggattaaac
     8881 gagcatacta atggactatt aagacaattt tttcccaagg gaacgaattt taaaattgtt
     8941 aagccagagc aggtggaaat agccgtaaac ttgattaaca atcgtcctcg aaaatgtctt
     9001 gactatcgta ccccgaatga ggtattctat aaaggtagat cagacagtga tgcaattcag
     9061 acttgaattg gccgaatcta atggtagtgc ctatcattac gctattaaca gttaggctta
     9121 attagggctt cctgaacaaa taatacaggg caatcagtac atagatttcg ccatttttgc
     9181 cgagcaatga taacttttat agctttttat ctatggataa aataatttag atcaattgaa
     9241 ctctaggtta gatgtggtat agactaagat tagtaattta tactcagata gttatgagac
     9301 aatccgatcg agaaataata attttagcgg ctgtggcatc cctcttagcg gtggtggcgg
     9361 aagtggcaat cgctttttag ataaaaaaaa ctgatttatg aggttaaagt cgggcaaaat
     9421 tggcaatcaa tgccgaggaa agatatcgat aaaatgcttg taacggtggc agcaattgcg
     9481 atcgcctcga tgagcgaaat taaagcatga atattagtgt aaccggtcag gtaaaaagaa
     9541 tcgaaatagg cacgggaact tgggcattaa ttgccgataa cggtcaaacc tacgaattaa
     9601 tggaacctcc cgccgaatta acccaaaaaa aggcaaaagt gacagttata ggtcaaattc
     9661 gctcagatgt gatgactctg gccatgatcg gaccggtttt acaagtgatc tcctttcaat
     9721 ccttgccatg aagccgcaaa atactgctta aaaacgggca aaaactactt tttaagcttg
     9781 taatctcgaa aaattcccgt aattgtcctt cagctatccc agaaagaaaa attgtcaaga
     9841 agaccctaaa ggttgatttt ttcgagaata atctcgaaat tttgaataat aatactacaa
     9901 tagacagcaa ctttgtctta ggaacatcgg aatcacctaa accgatctat cggattggtt
     9961 taactcgtag ttacctaagt tggcgtgagg agaggactga atgaatttgc gcgataatgc
    10021 tttaacgatt ctgcaagaaa cgagtcgaac tttttatatt cccataagtc gtttaccaga
    10081 tcgcctgctc gaggcagtag cctcagctta tctctgtatg agggcgatcg atgaagtaga
    10141 agaccatccc gacctagata acttcagtaa atcccagata ttaagacaaa ttagcctgaa
    10201 cttacaagca ggtactgaaa attccaccaa agaggacttt gcttttggct tagtccatcg
    10261 ggagattttg ccggaagtaa ccctgagaat tggcgaatgg gccctgttag cgccagattc
    10321 gatcgccccc cgggtctggg atgccacggc ggccatggcc gatcgcatgg cctactgggc
    10381 tgggaataac tgggatattc gcacagaaat agaattagat caatatacct ttagtgtggc
    10441 cggtgccgtg ggtttattgc tgtctgacct ctggaattgg tacgacggca ctcaaactaa
    10501 tcgcagttat gccatcggtt ttggtcgggg tttacaagcg gttaatattg cccgcaacca
    10561 tggcgaggat ctgcgccggg gggtaagttt tctgccccag ggttggacaa ttagtgatat
    10621 tcataattat gcccgtcgta atctgaaatt agccgattat tacactcaat cgctgcctaa
    10681 tgggccggcc cttgattttt gtaagattcc cctcgcttta gcccatggca ctctcgaagt
    10741 cctagcgtta ggtaaggaaa aactcagccg cagcgatgtc atcgctttgg tacaacaaat
    10801 tacccgatag aagaaatcac aattagggtt ggctgaataa atctaaaaac cttgttgggt
    10861 aagactttta gactttttgt caatcaaaaa gtaccggact gggagtgatc agggggaaaa
    10921 tccctggact ttttccctga aaattaggta gttgaccacc tgaaaatcgg taaaacccta
    10981 caccccacac cccacacccc acaccctgcc cccaagaaaa actttttgcc gcaaacccca
    11041 attaaaattg cccacgatcg ctcttccaaa gtcaggaatc acaagcgtgg gcaacaaacc
    11101 ggtggtgttc agtaacttag aacaaaccga gagttaaaga tgtatcgatg gggaaagtgg
    11161 caccaatacc taaccaaatc gtcaccacag taccaaatag gaagatagcg gtagcgacgg
    11221 gacgacggaa aggattttgg aatttattga cgctttcaat aaagggaact aacatcaagc
    11281 ctaggggaac gccggccatg caagcgatac ctaagagttt gttaggaaga atacggagaa
    11341 tttggaaaac gggataaaga taccactctg gcagaatttc tagaggagta gcgaaaggat
    11401 cggcaggttc accgatcatg gtgggatcca taatcgctaa accggtgcag agtccgatcg
    11461 taccaaagat aaccaccggg aagacataga gtagatcgtt aggccaagcg ggttcaccgt
    11521 agtagttgtg acccatccct ttagccaact tagcgcgcag agcgggatcg ctgagatcgg
    11581 gcttttttaa tgtggacatg gataatgtct cctaaaaatg atttcggatc gacagagata
    11641 tttttctgaa ttatagaggc ccagaaatgc cttgtttacg gatcatcaag aaatgtagca
    11701 acatgaagac cgcgatcgac cagggtagta caaaggtatg taagctgtag aaacgggtta
    11761 aagttgcttg accgacgctt tcgctaccgc gcatcagggt gacgagttga tcaccgacga
    11821 cgggaatagc agcaggtaca ccggaaacaa ttttaacggc ccagtaaccc acctgatccc
    11881 agggtaagga gtaaccggtg acaccgaagg taacggtgat tacggccaag acaaccccag
    11941 ttacccaggt taattcgcga ggttttttga agccaccggt cagatagacg cggaaaacgt
    12001 gcaggatcat cattaagacc atcatactgg cagaccagcg atggatagaa cgaattagcc
    12061 aaccaaagtt gacttcgttc atgatgtact gtacggagga gagggcctct gctacagtgg
    12121 gtttgtagta gaaggtcatg gcgaaaccag tggcaaactg gatcacgaag cacacaaggg
    12181 tgataccacc cagacagtag aagatattaa cgtggggggg aacgtatttg gtgctgatat
    12241 catcagcgat cccttgaatt tctaagcgtt cctgaaacca ttgataggct ttagaatccg
    12301 ttacttgttt ggtaaacatc gcgtaaccag taagaaaaat gaaagattta aggtgtgacc
    12361 tttaaaaaat gtaacacagt tttcagggct aattctcgct cttgtcaggt ttctccaccg
    12421 gtggggatca attggcgatc gctagacccg aaaaacgtaa cttcctaaaa tttcccttaa
    12481 aatgtctcct agactacaaa aaatcgggcc aattgtccga cactgggaat aggtaaggtt
    12541 gacagctagt tactgatttg ctcacattga cctacatacg ccactattta ggcgaacgtt
    12601 tgtgacccta agaaaagctg ttgagctatg gggattacat cccaatacat taagagaata
    12661 tgccgatgaa gggaaaatca aaagtatcaa aaacgaagca gggcaacgat tgtacgatgt
    12721 ccagtcttac gtcaatggtg cagctagagc ttcccttgtt tgctactgcc gagtcagttc
    12781 aatcaagcaa cgagatgacc ttgagagaca agtcagcttt atgcgcggat tctatccaga
    12841 ggcagaaatc atcaaagaca tcggtagtgg gcttaacttc caaagaaaag gacttagagc
    12901 catactggac agattattgt ggggaaataa actcacaatt gttatgtcca cactggacag
    12961 attatgccga tttagattcg agcttataca gtacatggtc gaacaaaacg gtggcaaaat
    13021 cttggttctc gaccaaactg ttcacagtcc cgaatcagaa cttacagcag acttgttata
    13081 aatacttcac atcttctctt gccgaatgtc cggactcagg aagtacgctc aaaaaatcaa
    13141 agaaagtgcg gatttatcca acaacagaac agaagaaact gatgaaacac tggtggggag
    13201 tatctcggtt tgtcttcaac caaaccatta aatatctaca ggaaccagat agaaaagcta
    13261 attggaaggc tatcaagacc ggaatcatta atgatttacc agaatgggct aaagaagtcc
    13321 cctaccagat taaatcaata gcagtcaaag acgcttgtga agccgttaaa aatgctcaaa
    13381 gaaaatatca aaaaactggg gaaatccaga aaataaaatt ccgttcccgt aaagataaac
    13441 agcaatcatg ttatattcct aagtcagcca tttcagaaaa aggaatatat cacacaaaat
    13501 tagggcaaat acgcttcacc gaatcactgc ccgacaattt tggggatagc cgattagtat
    13561 ccagaaatgg acaatactat ttagctattc cccatgaggt gcaacgatgc gaatccgata
    13621 accaaggacg cattgtcgcc ctagaccccg gtgttagaga ttttctggca ctattctcgc
    13681 ctaatagttt tggctcaatc gggagtcagg atataggtaa aatccaacga ttatgctatc
    13741 accttgatga tttaatttct cgttctacca aagttaatgc taaaaccaga aaccgttatc
    13801 gaaaagcagc tagtcgacta agaaataaaa tcaggaattt agtagatgaa cttcatcatc
    13861 aagttgctag attcctagtt gataactttg atgtgattct tttgcctact tttgaagtgt
    13921 cagaaatggt tctaaaaaat agcaggaaga ttcgctcaaa atctgttaga cagatgctta
    13981 actggtctca ttaccgattt aagcaatttc tcaagcataa ggcttttgag atgggtaaat
    14041 tagtaataga ctgctgtgaa gcctatacgt cgaaaacagt tagctggact ggtgaattaa
    14101 tacataactt aggtggaaaa aaagtcatta aatccaagat tgatggcaag acgatggata
    14161 gagatattaa tggcgcacga ggaatttttc ttcgtgcttt gggagatacc ccctctttaa
    14221 gagactctct tagagtgcat tgttaatgta tgttagcaaa aaagtatcgg tatggaatga
    14281 ggtcagcgtg ctattaaata cctacgatcc cgaaggcttc tacgacgaac tattcttaga
    14341 tgatggtcaa cctcgtcccc acgcgtcccc tttaatcaaa tggttgcaga atctgccgcc
    14401 ccgagaactt caacaacacc gggaaaccgc tcagtcggcc ctgtttgaat tgggggtgac
    14461 tttcaatgtc tatagcgaca atcaaggggt ggaaaaaatc tttccttttg atattattcc
    14521 ccgaattgtc tcggctgagg actgggatta tttagaaaga ggcctgaaac aacgcatcaa
    14581 agctctcaat ctttttctct ccgatatcta caatgaccag ttaatcatta aagatggcat
    14641 tattcccccc gaattaatcg cctcggcgac gggatttctg aaaccctgtg tgggactaaa
    14701 acccgccgga ggtatctggt gtcacatcac gggaaccgat ttagtccgcg ataaagatgg
    14761 tcaatggtac gttttagaag ataatttgcg ggttccttct ggtatttcct atgtcctcga
    14821 aaatcgtcgg gtaatgaaaa gcaccttccc cgatattttc cagacaatga atgttaaacc
    14881 ggtggatgat tatccttccc atctgttgga aactctcctc aatttagccc ctccccaatt
    14941 acctaatccc accgtggtgg ttttaacccc cggaacctat aattctgcct actttgaaca
    15001 ttcttttatt gcccaacaaa tgggggtaga attagtggaa gggcgtgatt tagtcgtctt
    15061 tgatggttat ctacaaatga aaaccaccaa gggattaaga cgggtagatg tggtatatcg
    15121 tcgcctcgat gatgactttt tagatccggc ggtttttcgt cccgattcga tgttaggtgt
    15181 acccggatta ttaaaagttt tccaggaagg tcgcgtcgct ttagctaatg ccccgggtac
    15241 gggagtcgcc gatgataagg ttatttatgc ttttgtcccc gatatgattc gttattatct
    15301 gggagaagaa gcgattttaa ataacgttcc tacctatctt tgttggcgag aaaaggactt
    15361 agattatgtc ttaaataatc tcgataagtt agttgtgaaa gccgctaatg agtcgggagg
    15421 ttatgggatg ttagtcggtt ctcaagctac ccccgaacaa agggcagaat tcgccccacg
    15481 cattgccgct aaccctcgca attatatcgc tcaaccggtg ttaagtcttt ctagggttcc
    15541 cactttaatt gacacggaag ttgaaggtcg tcatgtggat ttacgtccct atattctcca
    15601 tcgtggcgat gaaatttatg tgcatcccgg ggggttaact cgcgtggctt tgaaaaaggg
    15661 ttctttagtg gttaattctt ctcaaggtgg tggcagtaag gacacttggg ttttaaccaa
    15721 gtaatcaaga caataatcaa aattttggtg ggttacggcc aaaattaaat catgagtcga
    15781 gtgcataaat tattttcgcc caatccaccc taaccttttc actttttccc agttaattat
    15841 gcttagtaga gttgctgatt caatttattg gttaaatcgc tatattgaac gggcagaaaa
    15901 tgtcgccagg tttatggatg ttaaccttaa tttaatgtta gatgtaccta ccagtgtttt
    15961 gcaacagtgg gaacctttgg ttttaaccac gggagattta gaacaattca agaaacgtta
    16021 cggcacagcc accgcagaaa atgtgatcaa tttcttgaca tttgacacgg aatatagtag
    16081 ttcaattatc tcttgtttgc gctgggcgcg agaaaatgct cggtcagttc gcgagattat
    16141 ttcttcggaa atgtgggaac aggttaatgg ttttttctta atggtaaaag atgcttcctc
    16201 ttaccatcct caaactactt tacctaactt ttttacccaa gttaaattgg ctagtcaccg
    16261 ttttgcgggg gtgatggatg caacgatgac tcataatgaa ggttggcatt ttggcgttat
    16321 gggaagatta caggaacgcg ccgataaaac cgccagaatt cttgatgtta aatactatta
    16381 tctcttgcct tccgctcaat gggtaggaac ttctctagac caaattcaat ggattgcttt
    16441 actgcggtcg gtgagtgcct acgaaatgta tcgtaaatgt caacgacgga ttactcctag
    16501 tgacgtggcc gaatttttaa ttcttaatcg agaatttcct cgttcaattc acttttgtct
    16561 gcgggaatta gaccactgtt tgcatcaagt taccggtact cctgtaggaa cttggggcaa
    16621 ttcagtagaa agaagtttag ggcgactttg tgccgaaatg agttatctaa ctatcgagga
    16681 tattattgaa atgggattgc atgaattcct cgaccatatt caaaaaagag ttaatgatgt
    16741 gggaataaaa atgacagaaa ctttctttgt ttttaatccc gaacttgcct cacctctgcc
    16801 agtaaataac tttcaatatc aatcccaatt aataacttaa tgcgttatca cattcggcat
    16861 caaaccaact atttctataa tcaacccgtg attttatcgc ctcatctgct gcgcttgcgg
    16921 ccaaaatccg atggtgggca aactttgcga gagttcacac tcaaaattaa cccggaacct
    16981 ttaacaataa ctaatattat cgacctcgat ggcaattctt gtttacaaat ttggtttaat
    17041 caaccgacag aagaattatt gattgatgtg gtgagtgaag tggaaaccaa tggggttaat
    17101 ccttttcagt atttgttgga accttgggcg acctgtttac cctttgatta tcccatttcc
    17161 ctccacagtc aattacaacc ctacagacaa ttttatgggt tagctaatga ccccgatgtg
    17221 acagcattag cccaagaaat tatgcaggaa actgagggac aggtgctatc tttcctgttt
    17281 aatcttaatg acaaaattta ccgcaattgt cagcaaatta ttcgtcttac cggggaaccc
    17341 tatctacccg gaaacacttg gaaaaataag cagggttcct gtcgcgattt tgtggtttta
    17401 tttatggatg tgtgtcgctg tgtgggattg gcttcccgtt ttgtcagtgg ctatcaagaa
    17461 ggagatttag accaagaaga aagacattta cacgcttggg tagaggtgta tttgccgggg
    17521 ggcggctgga aaggctatga tcctacccat ggattagcgg tgagtgaccg tcatattagc
    17581 ctcgtttcta gcccaattcc ccgttattgc gcgccggtag agggaaatgc tcgaccggtt
    17641 agtctgggtc atcaagtgat agaatctcag ctatcatcga caatttccat cgatttgtta
    17701 tcttgaggat gattctttat ggctaaagga tttggcacag caacaaaagg acatttaggc
    17761 tatgttttat tgtttatggg gctaataata gtttagatgg ggagggagag gagtttattg
    17821 gggttactaa tctgttagaa atggcgcgag tttggactaa aaaaaatgcg gcgcgtgagg
    17881 cgataatcaa atatgcagat tttttgattg aacagttaga agaaactccc caagtgaata
    17941 ttcagattag aagtttaaag cgtttagcca acggcaagtt ggttacggaa gtgttagagg
    18001 agttaacttt tgaacaaaat tcggcttctt ctagatagag tttgcggcaa aaagtttttc
    18061 gtggcggcag ggtgtcgggt atcgagcagg actctatcgt tcatagagat cctcccttcg
    18121 ccaatccctt ttacccggtt gtgatgggcg gttttcgaga ccggtgataa aggccttttc
    18181 tcgctcccaa gcgctcaagt tcggggtagg agatttgacg gaaattatgt gttggtcaat
    18241 cgcctgtcgg atgatctcgg cctcgctcag tcccgtctgt cgggccttct ctttcagtaa
    18301 attattctgc tcggcttcga tataaatttg cttgcggatt ttgctgacca tagccagttt
    18361 aattatatac attacattat acattactat aaccgtcatg aatcgtaatc gccagttttt
    18421 ctatatatct gaacttcccc acatacatat ctactacaat agccgaaaac ccctatctat
    18481 caatggatac acccattaac ctttcttaat ctactcatgt ttatggtaat atttcttaat
    18541 agacggggat gaagtatatt tgctaaagtg atagccatgt atatggagaa agttcctaaa
    18601 cgtaattctc ctctgcaaga aaaggcctga gatttattag gggtttcctt gatttgtacc
    18661 cagtaacggt ataggagaaa ttctcttaat ccctcattta gatttattca gccagcccta
    18721 gatagagagc catttaagta attttctatg gagagttaaa ttatgtcgtc aactgcctac
    18781 gaaactgatt ataatcaatg gctaaaagag acggtaaaac agttacaaga gcgtaatttt
    18841 gagcaggtgg attgggataa tttaattgca gaaatcgaga gtatggggaa aagtgataga
    18901 cgagctttaa tgagtctgtt aacccgtctt atcgaacatc ttttaaagct ggcttattgg
    18961 caagaagaaa aaaaacggtc aggtaatcat tgggcggcag aaatagtcaa ttttcgcgcc
    19021 caaattcagc atcgtttaga agatagtccc agcttacggc cagaattagc agctatgtat
    19081 gataaagttt atccagtggc gattaaatcg gtttctcagc ttttttccct caattcagac
    19141 gctcatatat ccctcgaaca aacattagat gataattggt ttcccccggc tgaaaaatag
    19201 ttcttaatta ggagtaatac taaagctgaa taaatgtgaa atataaacga gataagggtt
    19261 ttagtgtcct gtttcgctcc cacaggtgca agattttgag agaatcgtgg ttcaaaacct
    19321 tgggtcttca tcggcccgcg tcctgtaggg gcgaagcatt cgggcaataa cctatggtga
    19381 aaccgtagat tttctatccg aatgcttcgc ccgtactttt tgccgcaaac cctaatcagt
    19441 ttttaataac cagatttagt ataatttgca tagtcaacag cttacccacg actataaaat
    19501 tagcatccat cttgcggggg tattaccctt gttaataccc cctatttttg gttcggtata
    19561 gtgttaagct tctcccgtga ttgataaacc ctctacccaa acgtagggac aaacaccact
    19621 gggagtaata actgcttccg gttccagata aacaatcgat ttcagcagtg ttaaaatatc
    19681 cccagctacg gtagcggaat cgatgctaac gcgctcattt ttgttcacta accaaccatc
    19741 aaagggcaaa gagaaggaac cttgtagaga attaactcct gcatgaagtg cctgtaaact
    19801 atcaattaaa accacatttt ctgctgtttc caaagaatag ctttttggtt gattagcact
    19861 agcaaaaacg tgataaaaat ggggactaac cgttactttt gacccaatgt taccgttgcc
    19921 agtcggttct gtattcatcc gtttagcggt aatcgtgcta tggagaaaat tactcaaaat
    19981 cccctcttta attaattcca cacgacgggt gggagtgcct tcactatcaa aagtttctgc
    20041 ggcaacatta gctggatgga gggcatcatc acacaaattt aaggccgtag cggcgatttg
    20101 tgttcccaaa gattcaggag tagaaagact ctgtttatca aggacatttt gggcattaaa
    20161 cagattagaa aatgctccca ttagactcaa aaaggcgcgg ggggcaaaaa ccaccaaata
    20221 tttacccgtg gtaatcggtt gatagtccag atggctaaca gttttggtaa tcgcttcctc
    20281 gatgcaacca tcgatatcta atttttccag tcccggacta acccgaaaag caccagcgct
    20341 gcggggtttt ttgccttcct gttcggtttt ggtgtataaa tacacactgg cataactacg
    20401 cgcttcctga cgcattgccc cgtcgctatt gagataaaaa cgtctgacct gctgttgtcc
    20461 caaaccatta tagggaacac tggcaatagc cggatgggcc gctagtaggg atttttcggc
    20521 ggctaataac ttatcgacga gagtggatac agctgcttga ttatacattt cgctggccac
    20581 ttctgtggta ggtgcagtgg cggcaggact aaaatcgggg acattttcct taacgccaaa
    20641 aaagctggct tcttgggcgg ttttcagggc taattgcagt cccaccgggt ccacatcggt
    20701 ggtcgaggtg acaccgactt gattatcccg attccagacg cgaacgataa cgctggaacg
    20761 attggaagct tgtacctgtt tgggttcccc ttgaaacaca tccacatcgg
//
