LOCUS       NZ_AP024565            20930 bp    DNA     circular CON 26-SEP-2024
DEFINITION  Microcystis aeruginosa strain NIES-88 chromosome, complete genome.
ACCESSION   NZ_AP024565
VERSION     NZ_AP024565.1
KEYWORDS    RefSeq.
SOURCE      Microcystis aeruginosa
  ORGANISM  Microcystis aeruginosa
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Chroococcales; Microcystaceae; Microcystis.
REFERENCE   1
  AUTHORS   Phan,C.-S., Matsuda,K., Balloo,N., Wakimoto,T. and Okino,T.
  TITLE     Argicyclamide A-C unveil enzymatic basis for guanidine
            bis-prenylation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5478552)
  AUTHORS   Matsuda,K. and Wakimoto,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2021) Contact:Kenichi Matsuda Hokkaido University,
            Graduate school of phrmaceutical sciences; Kita 12, Nishi 6,
            Kita-ku, Sapporo, Hokkaido 060-0812, Japan URL
            :https://www.pharm.hokudai.ac.jp/tennen/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 87x
            Sequencing Technology             :: DNBSEQ; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019704275.1-RS_2024_09_26
            Annotation Date                   :: 09/26/2024 11:16:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,602
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,071
            CDSs (with protein)               :: 5,071
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 110 of 479
            Pseudo Genes (incomplete)         :: 380 of 479
            Pseudo Genes (internal stop)      :: 101 of 479
            Pseudo Genes (multiple problems)  :: 91 of 479
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 14:54:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1623268
            Orig. end                         :: 1644198
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024565.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated at DFAST https://dfast.nig.ac.jp/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20930
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="5"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20930
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20930
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10930](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10930)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    complement(834..850)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 22.1"
                     /tool="antismash"
     gene            1270..1764
                     /locus_tag="LC603172_RS07970"
                     /old_locus_tag="MAN88_15410"
     CDS             1270..1764
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012264881.1"
                     /locus_tag="LC603172_RS07970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15410"
                     /product="hypothetical protein"
                     /protein_id="WP_260324536.1"
                     /transl_table=11
                     /translation="MWKKLAQAGFLCLRWNYIVQRVDWVIPFTDRELQKAWRENQRAAK
                     VKTITNAHRLLLFYSVECGLKAVLLKRQSKDCTDSCPELVEVQHDINKLLDKLRAGENL
                     KLPSQLSMNPLKKNQERKFSCAEINQMWRYGGCCDNIKDNELEKKLLDILSWIAQELQG
                     L"
     gene            1761..4502
                     /locus_tag="LC603172_RS07975"
                     /old_locus_tag="MAN88_15420"
     CDS             1761..4502
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002736152.1"
                     /locus_tag="LC603172_RS07975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15420"
                     /product="KGGVGR-motif variant AAA ATPase"
                     /protein_id="WP_061431288.1"
                     /transl_table=11
                     /translation="MRRRTLLTWFDIKRKIARETANGNDLPDGIVKISCFSSALEIGIQ
                     NQDDQPKANSILKEWFGDWYQESEQIIQLDIDNASIPVEFILIDNSSYFSPSIRPFWQE
                     VAYLKENTDSEQSDNFLPNAYQDKPEIIAFYSFKGGVGRTLHLAAYILALLARAKDINK
                     PIKILVIDSDLEAPGLTYWQRYENQQATVSLSNFLEVYQYPKPSLEVALSWVTQEIKKT
                     AKQQNKSTLYFLPAFIEEEELLDTPILPENLARNPSGAWEVGDAIYQLGQKLAVDYIFI
                     DLRAGLSEISSPILFDSRIQRFLVTTVNEQSWRGVRMVLEKIGLLAPSDLKVKDGNYYD
                     PSIIISFLTPEVRNLSAFENILEQLQSAYVQPKNESLYTTRLDIKETYFAQELLYVNNW
                     DEAQNKLSSTFLMELARQWAEDKLSNNSIASATPSDNSLDDVKKLRDTCNKYIYAEKGQ
                     GEDLLITEPLKNLARNFQDKLPNVISIGAKGAGKTFNYIQLSRLQSWENFLNKVDPQNQ
                     ENSLENLGLIFPLLQSQNLNDKAKNILEQARQNVNNVLNNDYQFSSSDLRDRITQSLSN
                     QDWTEVDWKIFWIKEISRVLGYNPENCQLHKLHEYLQQKNSKIIFLFDGLEDAFPNVAN
                     DNDNLQRKALKALIDHLPNNLSEIRQSNLGLIVFLRRDFLRYTITQNSQQFENLYRNYD
                     LSWNLESFLKLSYWLCIQSSVINANPQDLFDCSIEDLKEKLELLWGKKLGADNAREAKS
                     DNWIFAALTDFNGRLQARDIVRFLYHAANITVEKKEEIQFSKWSNTRLLPPQAIRRALE
                     PCSEEKVDESKQEYPIFKSWAESLPQYSDKKIPFSLEQFNLDADTVNVLEQMGVIYEDK
                     DKEDAVRYYMPEIFREGLGFSSQGARPRVLALKRKVLGKSNF"
     gene            4530..4967
                     /locus_tag="LC603172_RS07980"
                     /old_locus_tag="MAN88_15430"
     CDS             4530..4967
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS07980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MAN88_15430"
                     /product="restriction endonuclease"
                     /protein_id="WP_221275504.1"
                     /transl_table=11
                     /translation="MIDCYQLFTTDVAIINQKNAKKYSAECKLAKKGSFRLQGGIRPFI
                     EVKCMRSRTLGDKAAEQRSKLIGIPSTSLNIHKDQYIETDFDLVITSLANAFFQTNLET
                     GLFVWKPTPKEQIFLSKININNQEEALLKMYVARSKAPIQK"
     gene            5049..6035
                     /locus_tag="LC603172_RS07985"
                     /old_locus_tag="MAN88_15440"
     CDS             5049..6035
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012266743.1"
                     /locus_tag="LC603172_RS07985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15440"
                     /product="IS30-like element ISMae39 family transposase"
                     /protein_id="WP_221275333.1"
                     /transl_table=11
                     /translation="MSHQHLNIEQRNLLYQLSQEGNLSQRQMAVWLGCHQSTISRELKR
                     NQSSLGCYLPDTAQAQSETRRKNAKQPFKNVSESALELVKEGLKDYHSPEQIAGRLKRA
                     SQESLSHETIYQMIYQNYHGCGEYQKYLRRGLCQRKSRGGAKSKRGGIPGRVDISERPA
                     IADQKTEIGHWESDTMIGGNHLGVLVTHVDKASKFLVAGLAKNKTASEINRVTEKLFQE
                     IHPDKRKTFTCDNGKEFCGHQELSQKLGADFYFATPYHSWERGLNEHTNGLLRQFFPKG
                     TNFKIVKPEQVEIAVNLINNRPRKCLDYRTPNEVFYKGRSDSDAIQT"
     gene            complement(6391..7454)
                     /locus_tag="LC603172_RS07990"
                     /old_locus_tag="MAN88_15450"
     CDS             complement(join(6391..7298,7298..7454))
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611629.1"
                     /locus_tag="LC603172_RS07990"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="MAN88_15450"
                     /product="ISKra4 family transposase"
                     /protein_id="WP_221275363.1"
                     /ribosomal_slippage=""
                     /transl_table=11
                     /translation="MLSENEKRIKELCQELGQCLYEQSPIKKFNNLGEIEETVRDLMIH
                     YVNPEIGNFFVKTSTEETAGRTRKVKSILGELPITEKQAKKLEIKSRTQMSPMLEKNCL
                     LLSGDESYEKSAQKIKSLTGIAVSHSTQQRLVHRYAFEELPSNPEVEVEEMSLDGGKVR
                     LRTAKGKALIWRDYKAVSFHQLGVAAFFQDNSALLDLVNSQVLAKPLICLGDGHDGIWN
                     LFREIGEKQERIEILDWYHLIENLYKVGGSFQRIDEVKCFLWKGEVDAAISCFEGWSEP
                     QVENFIIYLNKHKHRIVNYGYLQAEGISIGSGSVESKIKQIAHRLKITGASWQSCNVPQ
                     VLRHRCAYLNGCLF"
     misc_feature    7496..7509
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 25.37"
                     /tool="antismash"
     gene            complement(7574..8386)
                     /locus_tag="LC603172_RS07995"
                     /old_locus_tag="MAN88_15470"
     CDS             complement(7574..8386)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159430.1"
                     /locus_tag="LC603172_RS07995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15470"
                     /product="DNA adenine methylase"
                     /protein_id="WP_260324537.1"
                     /transl_table=11
                     /translation="MSKIAKKLSNSPFRYPGGKFYARKLILACLPSHHKYCEPFAGGAS
                     IFFAKESVAENILNDKDEELMNCYRHIKDHLDSLIELLKDLNATKELHNYYKNEFQPAN
                     DLERAMRWYYLNRTSYSGIMKLENCYWGYGDKYSMRPENWPSHLKTTSEKLQNVNLSCL
                     DFEDLINQLPDDYFLFVDPPYFQADQNKFYSCFFSLEDHERLCKTLKKYQHRFKFLLTY
                     DNCSEIREMYDWSISLQDNEWNYTINRTDDQKNGLKLEHGYGSISLMQ"
     misc_feature    complement(8421..8437)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.64"
                     /tool="antismash"
     gene            complement(8599..8730)
                     /locus_tag="LC603172_RS08000"
     CDS             complement(8599..8730)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS08000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_260324538.1"
                     /transl_table=11
                     /translation="MSIVCDLERTNQLNLLPDKDLVDLCHPIEPYQVTKNPLHFYMN"
     gene            9123..9569
                     /locus_tag="LC603172_RS08005"
                     /old_locus_tag="MAN88_15480"
     CDS             9123..9569
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS08005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MAN88_15480"
                     /product="hypothetical protein"
                     /protein_id="WP_239698036.1"
                     /transl_table=11
                     /translation="MHDYLEGSLLSFDKLIKAQEQLAILLHEVDKTLANKNRPLINWRI
                     SQVHSGSIHLTLEGMPQDQITPSQISEVIKTVERGIVTILEHPIRPKYFSDRALESARS
                     LAILKKRDECMGDYPGFSVIKSSLSQPQKTPQLMTEHHQGDTDT"
     gene            complement(9531..9983)
                     /locus_tag="LC603172_RS08010"
                     /old_locus_tag="MAN88_15490"
     CDS             complement(9531..9983)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733310.1"
                     /locus_tag="LC603172_RS08010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15490"
                     /product="divergent PAP2 family protein"
                     /protein_id="WP_061431286.1"
                     /transl_table=11
                     /translation="MQDFEQVLHNQILLVSLLACFTAQGLKALIELIRDGKVSLRYLVS
                     SGGMPSAHSALVGALATGVGLQMGWSSPEFAIAALFAVIVMYDAAGVRQAAGKQARILN
                     QIIDEMFQEGKEFNEERLKELIGHTPFQVLVGLSLGIGIALVMLCH"
     gene            complement(10001..10930)
                     /gene="crtE"
                     /locus_tag="LC603172_RS08015"
                     /old_locus_tag="MAN88_15500"
     CDS             complement(10001..10930)
                     /EC_number="2.5.1.1"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0033386 - geranylgeranyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="crtE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203348.1"
                     /locus_tag="LC603172_RS08015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15500"
                     /product="geranylgeranyl diphosphate synthase CrtE"
                     /protein_id="WP_061431420.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.3e-61, bitscore:
                     206.6, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTRGEQGVEVRKNESFSLASYLEEKRLIVESALDSSLVIGNPAK
                     IYEAMRYSLLAGGKRLRPILCLATCELMGGSLEMALPTACALEMIHTMSLIHDDLPAMD
                     NDDYRRGKLTNHKVFGEDIAILAGDGLLAYAFEYVATQTRNVPPLQILEVIARLGRTVG
                     AAGLVGGQVLDLESAGKPDISAETLGFIHTQKTGALLETSVVSGAVLAGAKAEDIAKLA
                     RYAQNIGLAFQIIDDVLDITATKEELGKTAGKDLQAEKATYPSLWGIEVSRQQAQQLID
                     SAIGELSGYDEAAEPLRAIAEYIVNRKN"
     misc_feature    complement(11079..11092)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.2"
                     /tool="antismash"
     gene            11341..12738
                     /locus_tag="LC603172_RS08020"
                     /old_locus_tag="MAN88_15510"
     CDS             11341..12738
                     /EC_number="2.7.11.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002753470.1"
                     /locus_tag="LC603172_RS08020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15510"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_061431285.1"
                     /sec_met_domain="Pkinase (E-value: 4e-33, bitscore: 113.2,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLPGQVLGDRYQIQSQLGKQNGRRTFLAQDLKTGATVIVKLLIFS
                     PDFEWTDLKLFEREAETLKHLDCRSIPEYIDYFEVRTDTYQGFALVQSYIEAKSLKEQI
                     ENGRKFSELELQELAKSILGILIYLHGLNPPIIHRDLKPSNILLKDRSGHSIGNVYLID
                     FGSVQTVAQQEQATLTIVGTYGYMPPEQFGGRTVAASDLYSLGATLIYLATGIHPADLP
                     QKDGKIQLNNLANLSPAFVHWLGQLIEPSLEKRFISAQAANQALNTLHEMQLTASNLEK
                     PAGSRVQLQKNEEKIKISLPPEGFTPKLIFLGAFAIAWNAFLLVWTGGAVSIPLWISWF
                     FLLFSVPFWFVGLGMLYQIIYCLKGEEIITIDSQEIVAIQRIWGLTLPGKKKMARQHIN
                     KLVKADSYHSKDGDGDRVYVPMALTIWVGTKEYTISSKTKEYLSQPEIEWLAAELSQWL
                     GMPITNK"
     gene            12978..13553
                     /gene="def"
                     /locus_tag="LC603172_RS08025"
                     /old_locus_tag="MAN88_15520"
     CDS             12978..13553
                     /EC_number="3.5.1.88"
                     /GO_function="GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /GO_process="GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="def"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1275: peptide deformylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203351.1"
                     /locus_tag="LC603172_RS08025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15520"
                     /product="peptide deformylase"
                     /protein_id="WP_002748816.1"
                     /transl_table=11
                     /translation="MSATITVEKRKLEKPPLELHYLGDRVLRQPAKRIAKVDDSIRQLA
                     REMLQTMYSSNGIGLAAPQVAVNKQLIVIDCEHDKPENPPLILINPQIIGYSRELCKAE
                     EGCLSIPDVFLDVIRPQAIEVSYKDEQGKPRKLQANGLLARVIQHEMDHLNGVMFVDRV
                     DNDLALNEELKKHGFSVQAVKPVEKVTK"
     gene            13585..13782
                     /locus_tag="LC603172_RS08030"
                     /old_locus_tag="MAN88_15530"
     CDS             13585..13782
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203353.1"
                     /locus_tag="LC603172_RS08030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15530"
                     /product="hypothetical protein"
                     /protein_id="WP_061431284.1"
                     /transl_table=11
                     /translation="MTPKSGVLLLLSCIAAIAGVGCVFEISSGEPDLGNTTTSLILAAS
                     VPLTALFFWAAVKDTRANYK"
     gene            14044..14361
                     /locus_tag="LC603172_RS08035"
                     /old_locus_tag="MAN88_15540"
     CDS             14044..14361
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203355.1"
                     /locus_tag="LC603172_RS08035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15540"
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="WP_061431283.1"
                     /transl_table=11
                     /translation="MTTTYKVEISHLGTTQTIEVREDQTILQAAYDAGIDLPSSCNAGV
                     CTTCAAQLSQGSVEQGDGMGLSPDLQKEGYALLCVAYPRSDIKLESGKEEVVYQRQFGK
                     P"
     gene            complement(14443..16065)
                     /locus_tag="LC603172_RS08040"
                     /old_locus_tag="MAN88_15550"
     CDS             complement(14443..16065)
                     /EC_number="6.3.4.2"
                     /GO_function="GO:0003883 - CTP synthase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /GO_process="GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002746783.1"
                     /locus_tag="LC603172_RS08040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15550"
                     /product="CTP synthase"
                     /protein_id="WP_061431419.1"
                     /transl_table=11
                     /translation="MSKFVFVTGGVVSSIGKGIVAASLGRLLKSRDYSVSILKLDPYIN
                     VDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTDTELSRLNSVTTGSIYQAVLNKERR
                     GAYMGGTVQVIPHITNEIKERILRVAKDTNPDIVITEIGGTVGDIESLPFLEAIRQFRK
                     DVGRNNVVYMHVTLIPWIPAAKEMKTKPTQHSVKELRSIGIQPDVLVCRCHLPLQPGLK
                     EKLSAFCDVPVESVITAQDASSIYEVPLNLEKEGLAQQTLELLNLGPRYPHLEEWENLI
                     RQMQSPGQKLEIAIVGKYVQLGDAYLSVVEALKHAAIALDSEIELRWVSAEDVDHDSAE
                     AHLSGVDGIVVPGGFGIRGVDGKIKAIEYARVNNIPFLGLCLGMQCSIIEWGRNVARLE
                     RANSSEFEEDTPNPVINLLPEQADVVDLGGTMRLGLYPCRLNPDSLAFSLYGQEVIYER
                     HRHRYEFNNAYRSLFFETGYLVSGTSPDGRLVEIIELPKHPFFIATQFHPEFRSRPNKA
                     HPLFSGFMKAALKSAEQKFSLIS"
     gene            16297..16650
                     /locus_tag="LC603172_RS08045"
                     /old_locus_tag="MAN88_15560"
     CDS             16297..16650
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002753862.1"
                     /locus_tag="LC603172_RS08045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15560"
                     /product="hypothetical protein"
                     /protein_id="WP_061431282.1"
                     /transl_table=11
                     /translation="MIDFNTLAEFSRNYCVSICAFLVPANLVTTLLTVLFLYFGRPTRQ
                     VFPIASLALLFALTMFLHVATWLMIGVVMAPTFILFGLGLTCFVINLQAISDRQSLEQL
                     LHTGVSRLARSFN"
     gene            16882..18255
                     /locus_tag="LC603172_RS08050"
                     /old_locus_tag="MAN88_15570"
     CDS             16882..18255
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775175.1"
                     /locus_tag="LC603172_RS08050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15570"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_061431281.1"
                     /sec_met_domain="Pkinase (E-value: 2.9e-35, bitscore:
                     120.2, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEVICTRPHCPKPRNQCPDLDDSNRLTSIEQKYCTTCGMPLILGG
                     RYLPERLLGQGGFGAAFLALDRYSPKKAKCVLKQFQPSGNLSPQALEKAKNLFFQEAHV
                     LEDLGREHPQIPDLYAFFPLTVKNQLTGKEEQFFYLAQEYIDGKNLEEILQQQQKPFQE
                     SEVKNILEELLLILKFIHENGSIHRDIKPSNIMLSKNGRLYLLDFGAVKQATNNPSNTQ
                     EKSTTIYSAGFAPPEQQLGNQVSASTDLYALAATCVMLLTNRDPDDLYDPQKQVWNWQN
                     YAPNISADLARVFNKMLLANPLDRFQSCGEVLTALNTPSPIPASPPPSPIPPSPPASPV
                     IRKTSRFSLGETFANAAFTGFEGTLLVIALNSLVPSVGIVIAFMILGGLVLALFKKVIE
                     GRDLPILAAITLAFLLIPQLQGTLSFPEIAIIAVMAAIGAIAITAFFRLIYRIILRLF"
     gene            complement(18247..19686)
                     /locus_tag="LC603172_RS08055"
                     /old_locus_tag="MAN88_15580"
     CDS             complement(18247..19686)
                     /GO_function="GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (59-211). E-value:
                     2.9e-11. Score: 35.0. Matches aSDomain:
                     nrpspksdomains_LC603172_RS08055_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733697.1"
                     /locus_tag="LC603172_RS08055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_15580"
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="WP_061431280.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2.7e-09, bitscore:
                     35.0, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.3e-10,
                     bitscore: 39.5, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSPESAPLISQLQKKVNSLDVPFYAPGHKQGEGIGEDLSNLLGK
                     SVFKADLPELPDLDNLFAPTGVIKEAQILAAETFGADKSWFLVNGSSCGIIAAILATCG
                     EGDKIILARNIHKSAISGLILSGAQPIFINPEYNPTIDLNLNITPQSLENALKLHPDAK
                     AVMVVSPTYQGVCCDLKTIAQITDHYSIPLLVDEAHGAHFAFHPDLPPAALSLGADMAI
                     QSTHKVLGALTQASMLHLKSDRISSEKVDRALQLVQTTSPSYLLLASLDSARKQMATQG
                     LDLLTKTLDLAASARKELNKIPNISVLDFPHSIPGCHWFDRTRLTVIVKDFGLTGYEID
                     DILREKYAVTAELPTLSQLTFIISIGNHREHINRLITAFQCLQSPDSTSLPPTPAPVTG
                     NLAISPRKAFFAPTEIVSRKNALDRLSADVICPYPPGIPVLMPGELISQEVLDYLQTIL
                     DLGGTITGGSDHNLTTFRVLK"
     aSDomain        complement(19054..19509)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LC603172_RS08055_Aminotran_1_2.1
                     "
                     /evalue="2.90E-11"
                     /label="LC603172_RS08055_Aminotran_1_2.1"
                     /locus_tag="LC603172_RS08055"
                     /protein_end="211"
                     /protein_start="59"
                     /score="35.0"
                     /tool="antismash"
                     /translation="NLFAPTGVIKEAQILAAETFGADKSWFLVNGSSCGIIAAILATCG
                     EGDKIILARNIHKSAISGLILSGAQPIFINPEYNPTIDLNLNITPQSLENALKLHPDAK
                     AVMVVSPTYQGVCCDLKTIAQITDHYSIPLLVDEAHGAHFAFHPDLPP"
     misc_feature    19726..19744
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.29"
                     /tool="antismash"
     misc_feature    19733..19751
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.29"
                     /tool="antismash"
     misc_feature    19740..19758
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.29"
                     /tool="antismash"
     misc_feature    19747..19765
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.29"
                     /tool="antismash"
     misc_feature    complement(19751..19769)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.01"
                     /tool="antismash"
     misc_feature    complement(20210..20219)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 16.8"
                     /tool="antismash"
ORIGIN
        1 caccaccatc ggttatggtt cccccaataa gtctaatacc gccgatgttc acggggcagc
       61 tttaggagca acggaaatcg agttaacccg gaaagcatta ggttgggaat acgagccgtt
      121 tgtggttccc gatgaggtgt taaatcgttt ccggcaagca gtggacaagg gagcaacggc
      181 agaaacggac tggaataagt tatttaacga atatcaggcc aaatatcccg cagaagcggc
      241 tttatttgaa cagttaacca ccggtcaact gccggaaggt tgggagcaag ctttaccggt
      301 ttataaaccc gaagataagg ctctcgctag tcgtaaacac tcggaaatct gcttaaatgc
      361 cctcgcgggg gttttacccg gtttaatcgg tggttcggcg gatttaaccc actctaacta
      421 cacggaatta gagcattttg gtaatttcca aaaaggcagt tatcaggagc gtaacgtcca
      481 ttttggcgta agggaacacg cgatgggggc gatttgtaac ggtattgctc tccataacac
      541 gggattgatt ccctacgggg caactttcct gattttcacc gactatatgc ggaattctat
      601 ccgtttatcg gcattgtcag aaactcgcgt catctgggta atgactcacg attccattgc
      661 cctaggggaa gatggaccga ctcaccaacc cgtggaacac gttgcttctc tgcgcgctat
      721 tcctaacctc ttagtcatgc gtccggggga tggaaccgag acttccggtg cttataaagt
      781 agcggtgagc gaaactaagc gcccgacttt attggctctc tcccgtcaaa atctgcccaa
      841 tttagcagga acttcccttg cgggtgtggc taaaggtgct tatattatca ccgattgtga
      901 aggtactccc gatgtcattc tcatcggtac gggtggcgag ttgtatctct gcgataaagc
      961 ggctgcggtg ttaaaagaag caggaattaa agcccgcgtc gtttctatgc cctgttggga
     1021 gctattcgag gaacaatcgg cagaatacaa agaatcggta ttgccgaaag cagtgaaaaa
     1081 acgtctcgcg gtggaagcgg gttcaaccat gggttggcat cgttacatca ccgatgaggg
     1141 ggatgtgttg ggggttgata ccttcggcgc ttctgctcca ggaggtgtga ttctagagaa
     1201 gtttggtttt accgtggata acgtggtagc gaaggcaaaa gcgttgttag gttaattaaa
     1261 actttggttt tgtggaaaaa gctcgcgcaa gcgggttttt tgtgtttaag atggaactat
     1321 atcgtgcaaa gggttgactg ggtgattcct tttacagaca gagaactaca aaaagcatgg
     1381 agagaaaatc aacgagcggc aaaggtcaaa accataacta acgctcatcg tttattgcta
     1441 ttttactccg ttgaatgtgg tctcaaagca gttttactga aaagacaaag caaagattgc
     1501 accgattctt gtccagaatt agttgaggta cagcatgata taaataagtt actagacaaa
     1561 ctgcgtgcgg gagaaaacct aaaacttccc tcacaattaa gtatgaaccc tttaaaaaag
     1621 aatcaagaga gaaagttttc ttgcgctgaa attaatcaaa tgtggcgata tgggggatgc
     1681 tgtgacaata ttaaggataa tgaattagaa aaaaaattgt tagatatatt aagttggatt
     1741 gcacaggagt tgcagggact atgagaagac ggacattact aacttggttt gatattaaaa
     1801 gaaaaattgc tcgcgaaacc gctaacggaa atgacttacc tgatggtatt gttaaaatta
     1861 gttgttttag tagtgcttta gagattggta ttcaaaatca agatgatcaa ccaaaagcta
     1921 actcaatatt aaaagaatgg ttcggagatt ggtatcaaga atcagaacaa attattcagc
     1981 tagatattga taatgcttct attcctgtag aatttatttt aatagataat agttcttatt
     2041 tttctccatc gattcgacct ttttggcagg aagtcgctta tttaaaagaa aatacagatt
     2101 cagagcaatc ggataatttt ttacccaatg cttatcaaga taagccagaa attattgctt
     2161 tttattcttt taaaggtggt gtgggaagaa ccctacattt agccgcttat attcttgctc
     2221 tcttagcaag agcaaaagat ataaataaac caattaaaat tttagttatt gactcagatt
     2281 tagaggctcc ggggttaacc tattggcaaa ggtatgaaaa tcaacaggca accgtatctt
     2341 tgagcaattt tttggaagtt tatcaatatc ccaagccatc tctagaggtg gcactctctt
     2401 gggtaactca agaaattaag aaaactgcca aacagcaaaa taaatcaact ttatattttc
     2461 tccccgcttt tattgaagaa gaagaactct tagatactcc tatacttcca gagaatttag
     2521 ctagaaatcc atcgggtgct tgggaggtag gggatgcaat ttatcaatta gggcaaaaat
     2581 tagcagtaga ttatatattt attgatttaa gagcaggatt aagtgaaatt tctagcccaa
     2641 ttttatttga ctctagaatc caaagatttt tagtaacaac agttaatgag caatcttggc
     2701 gaggtgtcag gatggtttta gaaaaaattg gcttactagc tcccagtgat ttaaaagtaa
     2761 aggatggtaa ttattatgat ccttcgataa ttattagttt tttaacccca gaagtcagaa
     2821 atttatcagc atttgagaat attttagagc aattacagtc ggcttatgtt caacccaaaa
     2881 atgagagttt atatactact agactggata ttaaagaaac ttatttcgcc caagagttgc
     2941 tatatgttaa caattgggac gaagctcaaa acaaattaag ttcaactttc ttgatggaac
     3001 tggcaagaca atgggcagaa gataaattat ctaataatag cattgcatca gccacacctt
     3061 cagataactc cttagatgat gtgaaaaaac tcagggatac ttgcaataaa tatatttatg
     3121 cagaaaaggg acaaggggaa gatttactca ttaccgagcc attgaaaaat ctcgctcgta
     3181 attttcaaga taaactacct aatgttatct caattggtgc taaaggtgcg ggaaaaacct
     3241 ttaattatat tcagctatct cgcctacagt cttgggaaaa ttttttaaat aaagttgatc
     3301 ctcaaaatca agaaaattcc ctagaaaatc tcggtttaat atttccttta ctgcaatcac
     3361 aaaatttaaa tgataaggcc aaaaatattc ttgagcaagc acggcaaaat gttaataatg
     3421 ttcttaataa tgattatcaa ttttcctcgt cggatttaag agacagaatc acgcagtcgc
     3481 tatcaaatca ggattggact gaggttgatt ggaaaatttt ttggatcaaa gaaatttcga
     3541 gagttttagg ctataatcct gaaaattgtc aattacataa actgcatgaa taccttcaac
     3601 aaaaaaactc gaagattata tttttatttg atggattaga agatgctttt cctaatgttg
     3661 ccaatgataa cgataacctt caacgaaaag ccttgaaagc tttgatcgat catttaccca
     3721 ataatttatc agaaattcgg caatctaatc tcggattgat tgtctttctg cgtcgagatt
     3781 tcttgagata tacaatcaca caaaactcgc aacaatttga aaacttatat cgtaattatg
     3841 atctatcttg gaatctagaa tcgtttctta aactttccta ttggctctgc atacaatcat
     3901 cggttattaa tgctaaccct caagatttat tcgattgttc aattgaagat ttaaaagaaa
     3961 aattagaatt actttggggc aaaaaattag gagcagataa tgcaagggaa gctaagtcag
     4021 ataattggat ttttgcagct ttaaccgatt ttaatggaag actgcaagcg agggatattg
     4081 tgagattttt gtaccatgct gctaatatta ctgtagaaaa aaaagaagaa atacaatttt
     4141 ctaaatggtc gaatactaga ctgttacctc cccaagcgat tcgacgagcg cttgaaccct
     4201 gtagtgaaga aaaagttgac gagtctaagc aagaatatcc catatttaaa agctgggcag
     4261 aaagtttacc tcaatatagt gataaaaaaa ttcctttttc tctagaacag tttaatctgg
     4321 atgctgatac agtcaatgtt cttgagcaaa tgggagtaat ttatgaggac aaggataaag
     4381 aagatgcagt tcgttattat atgcccgaaa tattccgtga agggttagga ttttctagtc
     4441 aaggtgcgcg tcctcgtgtt ctcgctttaa agcgcaaagt tttggggaaa tcaaattttt
     4501 aatggttcga gctttttggc aatgactata tgattgattg ttatcagctt ttcacaacag
     4561 acgtagctat tatcaatcaa aaaaacgcca aaaaatactc tgctgaatgt aagttagcca
     4621 aaaaaggtag tttccgtctt cagggaggta tcagaccttt tattgaagtc aaatgtatga
     4681 gatcaagaac cttaggagac aaagcagcag aacaaaggtc taaattgata ggaatacctt
     4741 caacaagcct aaatattcac aaagatcaat atattgaaac agattttgat ttagtgatta
     4801 catctcttgc caatgctttt tttcagacca atttagaaac gggactattt gtttggaagc
     4861 ccacaccaaa agagcaaata tttttaagca aaattaatat taataatcaa gaagaagccc
     4921 ttttaaaaat gtacgttgcc agaagtaaag cgccaattca aaaatgaaac gcatcaacct
     4981 caagagttga caaagaaagg ctcatcaggt actgtctgat cattaacaaa aacaaatcag
     5041 acccaaaaat gagccaccaa catcttaaca tagaacaaag aaacttgctc taccaactta
     5101 gtcaggaggg aaatttatct caacggcaaa tggccgtctg gctcggatgt catcaaagca
     5161 caatatcacg ggaattaaaa aggaatcaaa gttcccttgg ctgctatcta cctgacaccg
     5221 cacaagctca aagcgagaca aggcgaaaaa atgccaaaca accctttaaa aatgtcagcg
     5281 agtcagcctt agagttggtc aaagaaggat tgaaagatta tcatagtccc gaacaaatag
     5341 caggtcgtct gaaaagagcc agtcaagaat ctctcagtca cgaaaccatc tatcaaatga
     5401 tctatcagaa ctatcacgga tgtggagagt accagaaata tttacgccgg gggctttgtc
     5461 aaagaaagag tcgaggcgga gcaaaaagta agcgtggagg gattccaggg cgtgtagata
     5521 tcagcgagcg accagcaatt gctgaccaga aaaccgagat cggtcattgg gaaagtgaca
     5581 cgatgattgg aggaaatcat ctaggagttc tcgttaccca tgtagacaag gcctcgaaat
     5641 ttctagtagc aggattagcc aaaaacaaaa ccgcatcgga aatcaataga gtcacagaaa
     5701 aactttttca agagattcac cctgacaaaa gaaaaacctt tacttgtgac aacggcaaag
     5761 aattttgtgg acatcaagag ttgagtcaga aattgggagc agatttttat tttgctactc
     5821 cttatcattc ttgggagagg ggattaaacg agcatactaa tggactatta agacaatttt
     5881 ttcccaaggg aacgaatttt aaaattgtta agccagagca ggtggaaata gccgtaaact
     5941 tgattaacaa tcgtcctcga aaatgtcttg actatcgtac cccgaatgag gtattctata
     6001 aaggtagatc agacagtgat gcaattcaga cttgaattgg ccaagattta acagcaaatc
     6061 aaaccaataa tatcaattgt tctcgccaga aatgccacga taacaactgt aatttcatcc
     6121 ctaattatcc caaaattttc tttgatgtta atacggctga acctctacaa ccatggctcc
     6181 cgatcgaaaa aatagaggat ttacttgatt aaaattagga atcaagatac atcgaataaa
     6241 cttaattgta gaggtgtaaa tcttgcaggt ttctcaagat cataattagc aataaatacc
     6301 tccttgccct tgcgtctttc actctgataa ccatggggag catctcacct ttgcaataag
     6361 tagaaatgct taatgagatc aataaaaaag ttaaaaaagg caaccattta gataagcaca
     6421 gcgatgacga aggacttgcg gtacattaca agattgccaa cttgcaccag taattttaag
     6481 acgatgagca atttgtttaa tttttgattc aacagagcca gagccaatcg aaatgccctc
     6541 tgcctgcaaa taaccataat tgacaattcg atgtttatgc ttgttcaaat aaataataaa
     6601 attctccact tgcggctctg accatccctc aaaacaggag atagcagcat ccacttcccc
     6661 cttccataga aaacatttta cctcatcaat ccgctggaat gacccgccaa ctttatagag
     6721 gttttcgatt aaatgatacc aatccaatat ttcaattctt tcctgtttct ctccgatctc
     6781 acgaaataaa ttccagatac catcatgtcc atctcctaaa caaattaaag gcttggccaa
     6841 aacttgagaa ttaaccaaat ctaataaagc tgagttatct tgaaaaaaag ccgctacccc
     6901 cagttgatga aaactcactg ctttataatc acgccaaatt aaggcttttc ccttggcagt
     6961 tcttagtcgt accttaccgc catctaggct catttcttcc acttccactt ctgggttaga
     7021 cggtaattct tcaaaagcat atcgatgtac gaggcgttgt tgggtactgt gagaaacagc
     7081 aatccctgtc aatgatttga ttttctgcgc cgatttttcg taggattcat cgccacttaa
     7141 tagcaaacag ttcttctcta acattggact catctgagtc cgagacttta tttctaattt
     7201 tttcgcttgt ttttctgtaa ttggtaattc ccccaaaata cttttcactt ttctcgtccg
     7261 accggcagtt tcttctgtgc ttgttttgac aaaaaaatac cgatttctgg gttaacataa
     7321 tgaatcatta aatctctgac tgtctcctct atctctccca agttattaaa tttcttaatt
     7381 ggggattgct catagagaca ttgccctaac tcttgacata actctttaat tcttttttca
     7441 ttttctgata acattgaatt attcctccca tcgagagcta ttctgaattg taagttataa
     7501 ctattataat ctcatttctc tttgtgatca agttgaagat tttctagttt ttgactgcta
     7561 taagtattaa tactcattgc ataagtgaga tgctcccata accatgttct aattttaaac
     7621 catttttttg atcatcagtt cgattaatag tgtaattcca ttcattatct tgtaaagaaa
     7681 tagaccaatc atacatttct ctaatttcgg agcaattatc ataagtcaaa agaaatttaa
     7741 atcgatgttg atattttttc aacgtcttac ataacctttc atgatcttct agactgaaga
     7801 agcaggaata gaatttattt tgatctgctt gaaagtaagg aggatcgaca aataaaaagt
     7861 aatcatcggg taattgattg attaaatctt caaaatctaa acaagataaa ttgacatttt
     7921 gcagtttttc cgaagtggtt tttaggtgag aaggccagtt ttcaggacgc atagaatact
     7981 tatcaccata gccccaataa caattttcaa gtttcataat tcctgagtag gaagttctat
     8041 ttagataata ccaacgcatg gctctttcta ggtcattggc tggttgaaat tcatttttat
     8101 aataattgtg taattctttg gtagcgttta gatcttttaa taattcaatt agactatcaa
     8161 gatgatcttt gatatgtcga taacagttca ttaattcctc atctttgtca ttgagaatat
     8221 tctctgctac actttcctta gcaaagaata tagatgctcc ccccgcaaaa ggttcacaat
     8281 acttatggtg actaggaaga caagctaaga ttagcttacg ggcataaaat ttaccgccgg
     8341 gataacgaaa aggagaatta ctaagttttt tagcgatttt ggacattacc tttgatttta
     8401 gaggcttttc tttgagatta ttttaacaaa attacgatat tttctctagg aatgtcagag
     8461 aagtttggtt ttaccgtgga taacgtggta gcgaaggcaa aagcgttgtt aggttaatta
     8521 aaactttggt tttgtggaaa aagcttgcgc aagcgggttt ttttgtggtt ataggtcaac
     8581 ctagtttagg ctttagggtt agttcatgta aaaatgtaaa gggttcttcg ttacttggta
     8641 tggttcgata gggtggcata aatcgactaa atctttatct ggcaagagat ttaattgatt
     8701 agttcgctcc agatcgcaaa caattgacaa aaatcgcagt aatgtctgtt tctataaggg
     8761 tttcatccct tataaccccg tccattgcat aagacaaacc gaagaaccat gtaaagtagg
     8821 aagctttagt agttagccaa aacctttaca cttgagataa ttatcacgat tcctaggatc
     8881 atgactaaaa gggaaagccc tatatcgaag gaaacaatca agagcctaac attagatata
     8941 agtacctaag caaaattaat tacacatcta agccactact ccgtcagact tctggtgtga
     9001 ggaaacagtg aactgaaaac tcaaatccga tccctaatag ctgtctcccg tctcccgtct
     9061 cggagtctcc cgtctcggag tctcctgtct cggagtctcc tgtctcgact aggaaattaa
     9121 ttttgcacga ctacttagaa ggctcactgt tgtcttttga taaattgatc aaggctcaag
     9181 aacaattagc aatactactc catgaagtag ataaaaccct tgctaacaaa aatcgccctc
     9241 tgataaattg gagaatctct caagttcata gtggcagcat tcatcttact ttagaaggaa
     9301 tgcctcaaga tcagattaca cctagtcaaa tttctgaagt tattaagact gtagaaaggg
     9361 gaatagtaac aattttagaa catccgattc gccctaagta tttttctgat cgagctttag
     9421 aaagtgcacg atcattagca atattgaaaa aaagagatga gtgtatgggt gattaccccg
     9481 gattttcagt tattaaatct agtttatctc aaccccagaa aacacctcaa ttaatgacag
     9541 agcatcacca gggcgatacc gatacctaaa cttaaaccga ccagcacttg aaaaggagta
     9601 tgaccaatta attcttttaa acgttcctcg ttaaattcct tgccctcctg gaacatttca
     9661 tcgataattt gattgagaat ccgggcctgt tttcctgctg cctgacgcac tccagccgca
     9721 tcatacataa caataactgc aaaaagcgcg gcgatcgcaa attcgggact agaccaaccc
     9781 atctgtaatc ctaccccagt agccaaggcc cccaccagcg ccgagtgagc gctaggcatt
     9841 cctcccgacg agactagata gcgcagactc accttaccat cccggattaa ctcaatcagt
     9901 gcttttagtc cctgggcagt gaaacaagct aaaagagaga ctagcagtat ttgattgtgt
     9961 aacacctgct cgaagtcctg cataatctca ttcgtttaat ttaattttta cgattaacaa
    10021 tatactcggc gatggcccgt aaaggttcag cggcctcatc gtagccactc aattcaccga
    10081 tggcactatc tatcaactgt tgagcttgct ggcgggacac ttctatcccc cagagactag
    10141 gataggtggc tttttctgcc tgtagatctt taccggcggt tttgcccaat tcctcctttg
    10201 tcgccgtgat atcgagaaca tcatcgataa tttggaaagc caagccaata ttttgagcgt
    10261 aacgggctaa tttagctata tcttccgctt ttgcccccgc taaaactgcc cccgacacca
    10321 cagaagtctc caacagggcc ccggttttct gagtatggat aaaaccgaga gtttccgccg
    10381 aaatatcggg ttttcctgcg gattccaagt ctaaaacctg accccccacc aaacccgctg
    10441 ccccgacagt gcgacctaaa cgggcaatta cctctaaaat ctgtagggga ggtacattac
    10501 gagtctgggt agccacatac tcgaaagcgt aggccaggag accatccccg gctaaaatag
    10561 cgatatcttc gccaaaaacc ttgtgattgg ttaatttccc gcgtcggtaa tcatcgttat
    10621 ccatggcggg taaatcgtca tgaattaatg acatagtgtg aatcatttcc agggcgcaag
    10681 cggtgggtaa agccatctct agggaacccc ccatcaattc acaggtagcc agacataaaa
    10741 tcggccgcag acgtttgcca ccggcgagga gagaatagcg catcgcctcg tagattttgg
    10801 caggattgcc gataactaaa gagctatcta aagctgactc gacaattaac cgcttttcct
    10861 ccagatagga agccagagaa aagctctcgt ttttcctcac ttctacccct tgttcgcccc
    10921 tagtaaccat gtctttttgt cttgaaccga ctgcttttaa aactatatca ggaaactcca
    10981 cctcgatcgg aattaaactg gctgctccct tgtacactaa gcggttgact atctaaatta
    11041 ctccttcaga ctgcatggca gcaccggagt tgacacccag aacttttgta ctaaaaattc
    11101 ccagacggag aggagcaaac tttcttttct gatgcctatt ttctctttta acctgaaaaa
    11161 atacatccct cagcccagtc cgaggattag cgaaaaacgg gcgatataat taaatttcgc
    11221 cgcagttatc atcggagatg ctgaagtttt gatgattcgg tcaaacttcc gttttcttag
    11281 ctggcagttg agaaatttct cgattaacct ataattagga ttatcctaga aatatattct
    11341 atgctgccag ggcaagtttt aggcgatcga taccaaattc aaagtcagtt aggtaaacaa
    11401 aatggaagac gcacgtttct cgcccaagat ctaaaaacgg gcgctactgt tattgtcaaa
    11461 ttattaatct tcagtcccga ttttgaatgg acagatttaa aactttttga acgggaagct
    11521 gaaactctta aacatctaga ttgtcgctcg attcctgaat atatcgacta ttttgaagtt
    11581 cgcaccgata cctatcaagg ttttgccctt gttcagagtt atatcgaggc gaaatctctc
    11641 aaggaacaga tagaaaatgg ccgcaaattt agtgaattag agctacaaga attagctaaa
    11701 tctatcctag gcattttaat ttatctccat ggacttaacc cgccaattat ccaccgggat
    11761 ttgaaaccta gtaatatctt actaaaagat cgatcgggtc atagtatcgg caatgtttat
    11821 ttaattgatt ttggttctgt ccagactgtt gcccaacagg aacaagccac tttaactatc
    11881 gtcggaacct acggttatat gcccccagaa caattcggtg gtcgcaccgt tgccgcttct
    11941 gatttataca gtttaggagc gactttaatt tatctagcta caggcattca tcccgccgat
    12001 ttaccccaaa aagatggcaa gattcaatta aataatttag ccaatttaag ccctgctttt
    12061 gttcattggt tagggcagtt aatcgaacct agtctcgaaa aaaggtttat ttcagcacaa
    12121 gcagcaaatc aagctttaaa tactctccac gaaatgcaat taactgcctc gaaccttgaa
    12181 aaacccgcag ggagtcgggt acagttgcaa aaaaacgagg agaaaattaa aatatcttta
    12241 cctcccgaag gttttacccc taaattaatt tttcttggag cttttgccat cgcttggaac
    12301 gcttttttat tagtatggac aggaggagca gtttccattc ctctgtggat aagttggttt
    12361 tttctgctct tttccgtgcc tttttggttt gtgggtttag gaatgcttta ccagattatt
    12421 tattgtctca aaggggagga aattatcact attgatagtc aagaaattgt ggccattcag
    12481 cgaatttggg gtttgacact tccaggcaaa aagaaaatgg cacggcaaca tattaataaa
    12541 ctggtgaaag ctgatagtta tcatagcaaa gatggtgatg gcgatcgagt ttatgtaccg
    12601 atggctttaa ctatttgggt ggggactaaa gaatatacta ttagcagtaa aacaaaagaa
    12661 tatctgagtc aacccgaaat tgaatggtta gcagcagaat taagccaatg gttagggatg
    12721 ccaattacta ataagtagcc agttacaatc agcttttttc tcccttctcc ccactccccc
    12781 actgattact gattactgct tactgataac tgaaaaatct ccccactccc cacttcccac
    12841 cccctgatat ggacgaactg tctgtgatca gtgtaaaatt ttatgaaggg tctgggtttt
    12901 tttctccctt ttcctgagaa atcccccaga gccagtgact aagatcaaaa ttgtgcatcc
    12961 catcgataat taagtttatg agcgccacta ttaccgtaga aaaacgcaaa ttagaaaaac
    13021 cccctttaga actgcattat ctcggcgatc gagtcctgcg tcaaccggcc aagcggattg
    13081 ctaaagtcga tgacagcatc cgccaattag ccagagaaat gctgcaaact atgtatagtt
    13141 ccaatggtat cggtttagcg gctccgcaag tggcagtcaa taaacaattg attgtcatcg
    13201 attgtgaaca cgataaaccg gaaaatccgc ccctaattct gattaatccc caaattatcg
    13261 gttatagtcg ggaattgtgc aaagcggaag agggttgttt gagtattccc gatgtgtttt
    13321 tagatgtcat ccgtccccaa gcgatcgaag tgagttataa agatgaacag gggaaaccgc
    13381 gaaaactgca agctaacggt ttactagcgc gggtaattca acacgaaatg gatcatctta
    13441 atggtgtcat gttcgtggac agagtagata acgatttggc actcaatgaa gaattaaaaa
    13501 aacacggttt ttccgtgcaa gcggtgaaac ccgtcgagaa agtgacaaaa taaaggcatt
    13561 agttaattta ggtgaggagt gaagatgacc ccgaaaagcg gcgttttatt gttattatcc
    13621 tgtattgcag cgatcgctgg tgtgggttgt gtctttgaaa ttagttcggg agaaccggat
    13681 ttaggcaata ccaccactag cttaattcta gcggcaagtg taccattaac agccctattt
    13741 ttttgggcgg cggttaaaga cacgagagcc aattacaaat agaataactt cccctcggtt
    13801 atctaaaaat tggcataatt ttcatgagca aaagagagaa gtaaattctc tcttttgttc
    13861 gttttaacta cagggcaaat tgccagaaaa agttactact ttttcggatt ttatcatctt
    13921 tcatgacaat caattatctg ccgaccggtg aaaatctgat taggtaaaga tttccctcct
    13981 ctctcctcta tccgcaatct agaatgataa aataggtgaa gttcacagca tcattcatta
    14041 gctatgacca caacctacaa agtagaaatt tcccatttag gaaccacaca aacgatcgaa
    14101 gttagggaag atcaaactat actacaggca gcctacgatg cggggattga cttacccagt
    14161 tcttgcaatg cgggcgtatg tactacctgc gccgctcaac tgtcccaggg tagcgttgaa
    14221 caaggtgatg gcatgggatt atcccccgac ctgcaaaaag agggctatgc tttgttatgt
    14281 gtggcctatc ctcgctccga tattaaactg gaatctggca aggaagaagt agtttatcaa
    14341 cgtcaattcg ggaaacccta ggacaaactg ggggtaatat gagaaattct cgggggtttc
    14401 cccccaaaaa accagatttt gagccaatag aaccggcaaa ccctaactaa taagggaaaa
    14461 cttttgctct gcacttttga gggccgcttt catgaaacca gaaaagagag gatgagcttt
    14521 attgggacga gagcgaaatt cggggtgaaa ttgggtagca ataaaaaaag gatgttttgg
    14581 tagttcgata atttccacca aacgaccatc aggagaagtg ccactcacca gataaccagt
    14641 ctcaaaaaag agactacgat aggcattatt gaactcgtaa cggtgacgat ggcgctcata
    14701 gataacctcc tgaccgtaga gggagaaagc cagactatcg ggatttaaac ggcagggata
    14761 gagacctaag cgcattgttc cccctaaatc caccacatct gcctgttctg gcagtaaatt
    14821 aatcactgga ttgggtgtat cctcttcaaa ttcggaacta ttggcccttt ctaatcgggc
    14881 cacattgcga ccccattcga taatcgaaca ctgcatccct aaacagagac ccaggaaagg
    14941 aatattattg accctagcat actcgatcgc cttaattttg ccatccacac ccctaatacc
    15001 aaaaccacct ggtacaacaa taccgtcaac accgcttaaa tgagcttcgg ccgaatcgtg
    15061 atccacatct tcggcactta cccaacgcaa ttctatctcg ctatcaaggg caatagcagc
    15121 gtgtttcaag gcctctacca ccgagagata agcgtccccc aattgcacat atttaccgac
    15181 gatagctatc tctaattttt gaccggggga ttgcatctgt cggatgagat tttcccactc
    15241 ctccagatgg gggtatcggg gacccaggtt taataattct agggtttgtt gcgctaatcc
    15301 ctctttttcc agattgagag gcacttcgta gatactgctg gcatcctgag cagtaatcac
    15361 cgattccacc ggcacatcac agaaagctga gagtttttct tttaaccccg gttgtagggg
    15421 tagatgacaa cgacagacta acacatccgg ttgaatgccg atcgatctta attccttgac
    15481 cgagtgttgc gtcggtttcg ttttcatctc cttagcggcc ggaatccaag gaattagggt
    15541 aacgtgcata taaaccacgt tatttcgacc tacatccttc ctaaattgcc taattgcttc
    15601 taaaaaaggc aaagattcga tatctcccac cgtaccacca atttctgtga tgacaatatc
    15661 ggggttggta tctttggcca cccggagaat cctttccttg atttcgttgg taatatgggg
    15721 gattacctgc acagttcccc ccatataagc accacggcgc tctttattca ataccgcttg
    15781 ataaatcgag ccagtagtca cgctattgag acgggagagt tccgtatcgg taaaacgttc
    15841 gtagtggcca aggtcgaggt cggtttcggc cccatcatcg gtgacaaata cctctccgtg
    15901 ttgaaaagga ctcatcgtac ccggatccac gttgatatag gggtctaatt tcagaattga
    15961 gacggaataa tcccgacttt ttaacaatct tcccaaacta gcggcgacaa ttcctttccc
    16021 aatactggag acgactcccc cggtgacaaa gacaaattta ctcataaaat ttttcctgcg
    16081 atcaattata cctagcaagg ggtgagagtt ttcgggatgg ggcggtgtca ttttcccaga
    16141 tttcattata accggaggat ctataccccg atcctcgttt tagctccctc tcgaaaatct
    16201 attgcaccac agcctttagt caccttcaac ccgatcatct aaattttccg ctacaatcga
    16261 gagtactaag actcaggcaa ttgtaaagta atattaatga tagattttaa tactctcgct
    16321 gaattttccc gcaactattg cgtctcgatt tgtgcctttt tagtacctgc taacttagta
    16381 accacccttt taacggttct ttttctctat ttcggtcgtc ctacccgtca agtcttcccc
    16441 attgcttccc tagctttgtt gttcgccctc accatgtttc tccacgtcgc cacctggttg
    16501 atgattggtg tggttatggc ccccactttt atcctctttg gactgggttt aacctgtttt
    16561 gtgatcaatc ttcaagccat cagcgatcgc caaagcttgg aacagttatt acacaccggg
    16621 gttagtcgtc tggcccgctc ttttaactaa ggaaaagtgc cgagacttcc caccccacca
    16681 tgaagataac cgcccttatc cggctgaatt tttttcagcc aaagacttga caacctccaa
    16741 aatttctgct aaccttgtag tataatggga atccgtgtcg gcctacgaac gctcttttta
    16801 actctttctc aagaacaaaa attttaaccc gaattgccgg tagtagctaa actaagagca
    16861 gtaccacggg aaacagtgac tatggaagtt atttgtacgc gtccccactg tccgaaaccg
    16921 cgtaatcaat gcccagacct agacgatagc aatcgcttaa cctcgatcga gcagaaatat
    16981 tgtactactt gcgggatgcc attaatttta gggggacgtt atttgccaga acgtctattg
    17041 ggacaaggag gatttggggc ggcattttta gcgctagatc gttatagtcc caaaaaagct
    17101 aaatgtgtac tcaaacaatt tcaaccttcg ggtaatttaa gtccgcaagc tttagaaaaa
    17161 gcgaaaaatt tgttttttca agaagcgcac gttttagaag atttaggacg agaacatccc
    17221 caaatccccg atctctatgc ttttttccct ttaacagtca agaatcaatt aacgggaaaa
    17281 gaagagcaat ttttctatct ggcccaggaa tatatcgacg gtaaaaactt agaagaaatt
    17341 ctccaacagc aacaaaaacc ctttcaagaa agtgaagtta aaaacattct cgaagaatta
    17401 cttcttatcc tcaaatttat tcatgaaaat ggttccattc accgggatat aaaaccatct
    17461 aacatcatgc tatccaaaaa tggccgcctc tatttactcg attttggggc agtaaaacag
    17521 gctaccaata accctagtaa tacacaagag aaatccacaa caatttactc ggcaggattt
    17581 gcccctcccg aacaacaatt aggcaatcag gtatcggcat ccacggattt atatgcccta
    17641 gcagctacct gtgttatgct cttaactaat cgcgatcctg atgatctata cgatccccaa
    17701 aaacaggtct ggaattggca aaattatgcc cccaacatca gtgcagattt agcccgagta
    17761 ttcaataaaa tgttactagc aaatcccctg gatagatttc aatcctgtgg agaagtttta
    17821 accgctttaa atacaccttc accaattcct gcatctcctc caccttctcc aattcctcca
    17881 tcccctccag cttctccagt gattagaaaa acttcccgct tttctcttgg ggaaactttc
    17941 gctaatgctg cctttacagg atttgagggg acattattag taattgcctt aaatagttta
    18001 gtcccctcgg taggaattgt catcgctttt atgatcttag gtggcttagt tttggcatta
    18061 tttaaaaaag tgattgaagg gagagattta ccgattctag cagcaattac tctcgccttc
    18121 ctgttaattc cgcaactgca aggaacccta agttttcctg aaattgctat cattgccgtg
    18181 atggcagcca tcggagccat tgctattacc gcctttttcc ggttaatata tcggataatt
    18241 ttgcgattat tttaaaaccc tgaaagtggt taaattatga tcactaccgc cagtaatcgt
    18301 gccaccgagg tctaaaatag tttgcagata gtcgaggact tcctgagaaa ttaactctcc
    18361 cggcatcaaa acgggaatac cggggggata gggacagata acatcagcac taagacgatc
    18421 gagggcattt ttccgggata ctatctctgt aggggcaaaa aaagcctttc taggagaaat
    18481 agccaaattt cccgtgactg gggcaggagt ggggggtaaa ctggtagagt caggagattg
    18541 aaggcattga aaagcagtaa ttaggcgatt aatgtgttcg cgatgattgc cgatggagat
    18601 aataaaagta agttggctta aagtcggtaa ttctgcggtg acggcgtatt tttctcgcaa
    18661 aatatcgtct atttcgtaac cagttaagcc aaaatctttg acgattaccg ttaaacgagt
    18721 gcgatcgaac cagtgacaac ccgggataga atggggaaag tctaaaacag agatattagg
    18781 gattttattg agttcttttc gggccgaagc ggctaaatcg agggttttgg tcagtaaatc
    18841 tagtccttgc gtcgccattt gctttctagc actatcaagg gaagcgagaa gcaaataact
    18901 agggctagtg gtttgaacta attgtaaggc tcgatcgact ttttcggaac taatgcgatc
    18961 gctttttaag tgtaacattg atgcttgagt taaggcccct aaaaccttat gggtggactg
    19021 aatagccata tcagcgccga gagacagggc agccggaggt aaatcgggat gaaaggcaaa
    19081 atgcgccccg tgagcctcat cgacgagcag cgggatagaa taatgatcgg taatctgggc
    19141 gatagttttt aagtcacagc aaaccccttg ataggtggga gagactacca tcaccgcttt
    19201 agcgtcggga tggagtttca gggcattttc gagggattgg ggggttatat ttaaatttag
    19261 atcgatagtg ggattatatt cgggattgat aaagataggc tgcgccccag agagaattaa
    19321 gcccgaaatt gccgatttat gaatatttct cgccaaaata attttatctc cctcgccgca
    19381 agtggctaaa atcgccgcaa taatgccaca actggagcca ttgactaaaa accagctttt
    19441 atctgcccca aatgtctcgg ctgctaaaat ttgcgcttct ttaatcaccc ccgtcggggc
    19501 aaacaaatta tcaaggtcgg gtaattcggg taaatcggct ttaaagacgc tttttcccag
    19561 taaattactt aaatcttccc cgattccttc cccctgctta tgccctggcg cataaaatgg
    19621 tacgtctaaa ctattgactt ttttttgcag ttgtgatatt agcggagcag attctggcga
    19681 aggcattgtg agaagtggga agtgggggat ttgtcagtga tcagtaaaca gtaaacagta
    19741 aacagtaaac agtaaacagt aaacaataat cagtaaacag taattagtga agattgatcg
    19801 gcgaataata tcatttaaaa cttaaaactt atcacttatc actttaatct gataactgat
    19861 aactgataac tgatcactga taactgttaa ctatctgtgc ctaacatttg cagtttatat
    19921 aaactagcgt agaggccgtc ttcggttaat aaatcttcat gggttcccga ttcgactaat
    19981 tccccgcgtt taaggacaag aattcgatcg acatcgcgaa tagtggagag acgatgggcg
    20041 ataataatag cagtacgatt gactaaaatc tgttctaaag cttcttgaat taatacttct
    20101 gtccccacgt ctaaactagc ggtagcttca tctaaaacta aaatttcggg gttacgaata
    20161 gcgactctcg caaaagcgag taattgtttt tgtcctcccg ataaatttgc gccccgttcc
    20221 cgtaaaaggg tatgataacc atcgggtaac tcctcgataa agtgatcgat attagttaat
    20281 ttagcggctg ccttcacttg ctcaaaatca taattttccc ctaaagtaat attgcgagta
    20341 acatctccgg caaagagaaa agtttcttgg agaatgacac cgatataacg tcttaattcc
    20401 gcttggggaa gataacggat atcgatacca tccactagaa ttctgccctt acttggttcg
    20461 taaagacgac agagaagacg aataatcgaa ctttttcccg caccggtggg accaactaaa
    20521 gcgacttttt cccctggatg aatggtaaaa ttgagacctt tgataatata ttcatcgggt
    20581 ttataaccaa accaaacatt ttcaaaaata atctcgccag tttgacgttt ttctgggaag
    20641 taaatctctt tataattgtt agaatcggtg atttcaatgg gaatactaat taattcacca
    20701 attcgttcta tagcagtaaa acccgattga aacatagtaa atttttcggc aaactgacgc
    20761 aggggattaa agagacgttg ggagaataaa ataaaagctg ataaaacccc aaaatcaatg
    20821 gttttctgga gaataaaaat cccgcctaac cataatactc ctcccacggc aattaatccg
    20881 atccattcca aagtggccga gacagcagaa tcataaaaaa ttgttttatc
//
