LOCUS       NZ_AP024565            26898 bp    DNA     circular CON 26-SEP-2024
DEFINITION  Microcystis aeruginosa strain NIES-88 chromosome, complete genome.
ACCESSION   NZ_AP024565
VERSION     NZ_AP024565.1
KEYWORDS    RefSeq.
SOURCE      Microcystis aeruginosa
  ORGANISM  Microcystis aeruginosa
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Chroococcales; Microcystaceae; Microcystis.
REFERENCE   1
  AUTHORS   Phan,C.-S., Matsuda,K., Balloo,N., Wakimoto,T. and Okino,T.
  TITLE     Argicyclamide A-C unveil enzymatic basis for guanidine
            bis-prenylation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5478552)
  AUTHORS   Matsuda,K. and Wakimoto,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2021) Contact:Kenichi Matsuda Hokkaido University,
            Graduate school of phrmaceutical sciences; Kita 12, Nishi 6,
            Kita-ku, Sapporo, Hokkaido 060-0812, Japan URL
            :https://www.pharm.hokudai.ac.jp/tennen/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 87x
            Sequencing Technology             :: DNBSEQ; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019704275.1-RS_2024_09_26
            Annotation Date                   :: 09/26/2024 11:16:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,602
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,071
            CDSs (with protein)               :: 5,071
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 110 of 479
            Pseudo Genes (incomplete)         :: 380 of 479
            Pseudo Genes (internal stop)      :: 101 of 479
            Pseudo Genes (multiple problems)  :: 91 of 479
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 14:54:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1348842
            Orig. end                         :: 1375740
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024565.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated at DFAST https://dfast.nig.ac.jp/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..26898
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyanobactin"
                     /region_number="4"
                     /rules="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /tool="antismash"
     cand_cluster    1..26898
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /kind="single"
                     /product="cyanobactin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..26898
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:16898](-)"
                     /cutoff="20000"
                     /detection_rule="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /neighbourhood="10000"
                     /product="cyanobactin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..16898)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /neighbourhood="10000"
                     /product="cyanobactin"
                     /protocluster_number="1"
     misc_feature    complement(3..17)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.5"
                     /tool="antismash"
     misc_feature    514..533
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 21.61"
                     /tool="antismash"
     gene            1234..1434
                     /locus_tag="LC603172_RS06545"
                     /old_locus_tag="MAN88_12680"
     CDS             1234..1434
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS06545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MAN88_12680"
                     /product="hypothetical protein"
                     /protein_id="WP_061433031.1"
                     /transl_table=11
                     /translation="MKKVKILPIYLSFLILIYIISVMTVNLSSFPGLHGDEAWFGINAG
                     EILHHYFEGASHLCNEHKYLV"
     gene            1864..2838
                     /locus_tag="LC603172_RS06550"
                     /old_locus_tag="MAN88_12690"
     CDS             1864..2838
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002781226.1"
                     /locus_tag="LC603172_RS06550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12690"
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_002791129.1"
                     /transl_table=11
                     /translation="MKIKKYLKFWKYPRLVDERETLIEERDSLKDHLKSLNQEKQEMIR
                     EKNALYERLGQLSLTIPELQEISPKLLDISVIKNKARQFRVQLDAEKEKLAPDNFGWYP
                     YNTLTCFEILELLLTGKNRFLLELISEKPIVDIGCADGDLAFFLESLGCKVQVVDYAPT
                     NFNLMQGVKLLKTALSSSVEIHDVNLDSQFLLPEKSYSLVIFLGILYHLKNPYYALETL
                     AKSANYCLISTRVTKFNTVSNTANRVDLSAIPLAYLLDELEANNDSTNYWIFSNAGLRR
                     ILQRTGWEICDYITVGNTENSDPASRDGDERAYCLVKSRFYQD"
     gene            2873..3943
                     /locus_tag="LC603172_RS06555"
                     /old_locus_tag="MAN88_12700"
     CDS             2873..3943
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1226: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197655.1"
                     /locus_tag="LC603172_RS06555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12700"
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="WP_061433030.1"
                     /transl_table=11
                     /translation="MNYPPNPLLNRRSLLKLAALAGGSAGLATLLSSRLSYSADQNQAS
                     AENIEAIEEFLRQIRPVNSQNLQWTEYSFETVTVDRQGQIIDRRQGSARSWREPLGNGS
                     ELEMVAIPGGSFLRGSKNEERGDHTSSEKPENSLTTAAFFLSKYPITQAQWRAIAKLAK
                     INLELNPAPSFFQGDNLPVERVSWYEAIEFCQRLSRATGKDYRLASESRWEYACRAGTT
                     TPFYCGETLTSERANYFAVYDPYAEEPASAYLARTTPVGSFAPNNFGLYDMHGNVGEWC
                     ADSWHRNYEGTPPLDGSAWLDRDTRLQPRYRSARLVRGGSWGDEARHLRSANRTFCLPD
                     QHHSVLGLRVMADSTG"
     gene            4076..5353
                     /locus_tag="LC603172_RS06560"
                     /old_locus_tag="MAN88_12710"
     CDS             4076..5353
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1123: polyprenol-monophosphomannose synthase ppm1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002791126.1"
                     /locus_tag="LC603172_RS06560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12710"
                     /product="glycosyltransferase"
                     /protein_id="WP_061433029.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 9.8e-31,
                     bitscore: 105.2, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIQQSHLFLTPPTGDLQVPLSPPPPDDGNLEEEKNYSLSLSLVI
                     PTYNERQNIVQLVNILSQILDEALPNDYELIVVDDNSPDRTWEVAASLIPEYPQLRVLR
                     REGEKGLSSAVVRGWQVARGDILGVIDADLQHPPEVLLKLFEQTRAGADLSLASRHVEG
                     GGVSQWSLTRRFLSRGAQLLGLVILPNVVGRVSDPMSGYFMVNRQAIAGPILNPMGYKI
                     LLEVIGRGNIDRIAEVGYVFQERQDGESKVTWQQYIEYILHLLKLRSRGRFGGLKRRWQ
                     FPLARFLRFSLVGFSGVFVDMAIFYLLSDPTTLAWGVTRSKIIAAEVAIINNFLWNDRW
                     TFGDLSTGQNQWQQRCKRFLKFNLICLAGLILNVLLLNFFFNVLHINRYIANLIAIAIV
                     TVWNFWMNLKLSWRVTEVKPEKSGKP"
     gene            5599..6564
                     /locus_tag="LC603172_RS06565"
                     /old_locus_tag="MAN88_12720"
     CDS             5599..6564
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1123: polyprenol-monophosphomannose synthase ppm1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012266881.1"
                     /locus_tag="LC603172_RS06565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12720"
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_061433100.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 4.2e-14,
                     bitscore: 51.1, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEMTAIPPISLQNYRIAAIIPCHNEELTIAKVVSQFQTFLPEAA
                     IYVYNNRSSDDTVTEALKAGAIVRQEIQPGKGNVVRRMFADIEADIYILIDGDDTYEIA
                     AVRRLIERMLREQLDMVVGARREAAKSSQAYRPGHRAGNLFLTGLVKFLFGARLIDMLS
                     GYRVFSRRFVKSFPALSNGFEIETELTIHALELKIPFAEEPTLYGERPEGSQSKLKTFQ
                     DGWRVLGTAILLFKEIRPFLFFSLVSLILAVISLLLAIPVLFEYLETGLVPRFPTAILS
                     ASIMLLAFLSLTCGMILDSVSRGRKEMKRMAYLANSWLKG"
     gene            complement(6608..6973)
                     /locus_tag="LC603172_RS06570"
                     /old_locus_tag="MAN88_12730"
     CDS             complement(6608..6973)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197647.1"
                     /locus_tag="LC603172_RS06570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12730"
                     /product="DUF1815 family protein"
                     /protein_id="WP_221275493.1"
                     /transl_table=11
                     /translation="MFKRLAEQHRQLVKDLVMDLQALAIALENQGYLASCYTCGGQMNS
                     ASFMVGLGETHLIRFLVSDYGITWTEMRDDRELMKLEGAEAISQLQELANLIKYQISPA
                     EFIDKKPSSVLQQLETV"
     gene            complement(7266..>7616)
                     /locus_tag="LC603172_RS06575"
                     /pseudo=""
     CDS             complement(7266..>7616)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517330.1"
                     /locus_tag="LC603172_RS06575"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="RNA-guided endonuclease TnpB family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="QLSQAISDVSWGEITRQLAYKCRWYGRNYIEIDRWFPSSKRCSNC
                     GHIAEKMPLNVREWDCPDCGTHHDRDINASKNILAAGLAVSVCRATIRPEQSKSVKAGA
                     EPRKGKKQKPKS"
     gene            complement(8077..9000)
                     /locus_tag="LC603172_RS06580"
                     /old_locus_tag="MAN88_12750"
     CDS             complement(8077..9000)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS06580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MAN88_12750"
                     /product="hypothetical protein"
                     /protein_id="WP_061432706.1"
                     /transl_table=11
                     /translation="MMALSPFDESNKINIEAYFHWLVTRINHLPDSDINSLLQTYIAIV
                     ICHDIEPKILNESIKIVKNLTAKLILLSEHQFRSSNLILLSLAAISTYRLLGTQLPNIV
                     DHVKKVIDKLPEKSSDNSLNERPILFLAGTLNLIPKPSFKVMNYKPDLLALLNADDLAI
                     QKSITELENLTVYGIVRTPIYHGLIDVLEVILFEYLQNYNLKIALRLFRMLNYLNVSTK
                     YGIQKSFAFINNQQCPDGSFGFSELEIAKMDIEFQFNDLKNWFKLQNTFSYLWLTAEQM
                     NSDYRLLRDIGRGPIQGSSLYQFSRV"
     gene            complement(8997..9956)
                     /locus_tag="LC603172_RS06585"
                     /old_locus_tag="MAN88_12760"
     CDS             complement(8997..9956)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF046440.1"
                     /locus_tag="LC603172_RS06585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12760"
                     /product="DUF6895 family protein"
                     /protein_id="WP_061432707.1"
                     /transl_table=11
                     /translation="MLNYFDILLPSLLERTLDWITQHIDYFSLPNHKSSDREVKATIEH
                     KAFGELAIACLVMNRYPQVRQRQEFKVLIGHIVEELEKSNFTFNMTRRFGLFEFYLSIY
                     SCLKACDLKTTIEVEQLKILLQKMIDEDFIDAVERTPYSQMELSYYFKLGGFKHRLSDC
                     SSIYKYSSAFHLPSPIHLRITDAYNLTHIIFYLSDFGQQPLAPILLERYNEIYQYIILI
                     LGLYTKKKDWDLVAELLICCNFLQCDYFHPLIQLAGKSLGEIQNPQGFIPAKSRLSEKQ
                     ESNSEEVNLEKDTFRKNYHPTLVTFLASMFYLYGGNYL"
     gene            complement(10001..12019)
                     /locus_tag="LC603172_RS06590"
                     /old_locus_tag="MAN88_12770"
     CDS             complement(10001..12019)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002762755.1"
                     /locus_tag="LC603172_RS06590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12770"
                     /product="PatA/PatG family cyanobactin maturation protease"
                     /protein_id="WP_061432708.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 6.4e-23, bitscore:
                     79.8, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 1.2e-176,
                     bitscore: 586.4, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDIIDIPGITELWTRTLGSPQITVAVLDGPADLDRACFQKANIA
                     QIKPYWQEMLEVPQEYIDLYIDLENREEDSEEKAAKMESAIPAPFGDYLNFVSHATHIA
                     STIFGQHDSPVKGLAPNCRGINIPLGYDFDNFSSPLNLSRAINLALNAGANIIHIAVCI
                     PTQTGVAHDLLERAVRQCQDNNILLVAPGGNNEGECWCIPAVLPHVLTVGAMKDNGQPF
                     KFSNWGGEYQNQGVLAPGENILGAQPWSDKPLRKKGTSCAAPIVTGVAALLMSLQLQQG
                     QEPDAEAVRAAILNSAIPCDPEEVEEVERCLLGRLNVPSAINLVDKKPLLIVASSGFET
                     AWKIEAMAVTDSIAQPDPISSILEDSVMPTDSSPAITPSQTADSITPSARTNLVYALGT
                     LGYDFGTEARRDSFKQLMPRQSIPGYPAGTPPVLANPYDARQMADYLAQNPSEAKSLIW
                     TLNMELTPIYAIEPVGPFAYAVYELLQGLLQGEVQPENSSEYIDRVSIPARLTGKTVKL
                     LSGQEVPVIRPDGERGIYGWKVNSLIDAALEAVPVNEQPQLAAVLSNFLNRIYYDFSNL
                     GQTASDRALNFAATNIVQSVSVFSESILLGMELDSITVEKSPYCRLNSDCWDVALKFFD
                     PENNDRARKVYRYTLDVSDIFPVTLGGEAKTWWDSPN"
     gene            complement(12106..12978)
                     /locus_tag="LC603172_RS06595"
                     /old_locus_tag="MAN88_12780"
     CDS             complement(12106..12978)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04445.1"
                     /locus_tag="LC603172_RS06595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12780"
                     /product="LynF/TruF/PatF family peptide
                     O-prenyltransferase"
                     /protein_id="WP_061432709.1"
                     /transl_table=11
                     /translation="MINYANAQLHKSKNLMYMKAHENIFEIEALYPLELFERFMQSQTD
                     CSIDCACKIDGDELYPARFSLALYNNQYAEKQIRETIDFFHQVEGRTEVKLNYQQLQHF
                     LGADFDFSKVIRNLVGVDARRELADSRVKLYIWMNDYPEKMATAMAWCDDKKELSTLIV
                     NQEFLVGFDFYFDGRTAIELYISLSSEEFQQTQVWERLAKVVCAPALRLVNDCQAIQIG
                     VSRANDSKIMYYHTLNPNSFIDNLGNEMASRVHAYYRHQPVRSLVVCIPEQELTARSIQ
                     RLNMYYCMN"
     gene            complement(13721..13984)
                     /locus_tag="LC603172_RS06600"
                     /old_locus_tag="MAN88_12790"
     CDS             complement(13721..13984)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS06600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MAN88_12790"
                     /product="hypothetical protein"
                     /protein_id="WP_061432710.1"
                     /transl_table=11
                     /translation="MKNPTLLPKLTAPVERPAVTSSDLKQASSVDAAWLNGDNNWSTPF
                     AGVNAAWLNGDNNWSTPFAGVNAAWLNGDNNWSTPFAADGAE"
     gene            complement(14290..14469)
                     /locus_tag="LC603172_RS06605"
     CDS             complement(14290..14469)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LC603172_RS06605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_221275414.1"
                     /transl_table=11
                     /translation="MNQMKKKTLLPQLTESIKRPSINVPSSPMPELGGVETAIYIPGLG
                     GVLQGIPFASDDAL"
     gene            complement(14734..15144)
                     /locus_tag="LC603172_RS27775"
                     /old_locus_tag="MAN88_12810"
     CDS             complement(14734..15144)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04220.1"
                     /locus_tag="LC603172_RS27775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12810"
                     /product="cyanobactin biosynthesis system PatB/AcyB/McaB
                     family protein"
                     /protein_id="WP_081106828.1"
                     /transl_table=11
                     /translation="MIRNETPIREDIGRFLSIVFGLERTNQLNLLLDKDLVDLCPPIEP
                     YQVTKNQLKVKFKMIPIQAKPVKRPDVIAPHLAVDVVHNDGSRISDPTSINNLVVIRIR
                     LLHGANYNDPPYFQSKSYQQIKSSGLRGSLLK"
     gene            complement(15180..16898)
                     /locus_tag="LC603172_RS06615"
                     /old_locus_tag="MAN88_12820"
     CDS             complement(15180..16898)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015133055.1"
                     /locus_tag="LC603172_RS06615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12820"
                     /product="PatA/PatG family cyanobactin maturation protease"
                     /protein_id="WP_221275494.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 4.8e-23, bitscore:
                     80.2, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 1.5e-204,
                     bitscore: 678.3, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSLFFGSPFADRGQRLSQLDLSRAIEQAVSSGAHIINISGGQLT
                     DFGEAEDFLQRVTQLCEKNNILIVAATGNDGCPCLHVPAAIPSVLAVGAMDNLGKPMEF
                     SNWGKNYQTQGILAPGDNILGAKPGGGVVRLSGTSFATPIVSGVAALLLSLQLQQGEIP
                     DPHKVRDAILNSALPCDPNQIKSNYHCLVGKINISGAIELLQGGTMSELRETIAPAGCG
                     CDVVNNIEADVANTNEIEASANTSEISHSIPETALAASVISPSSSQNSLTSNVSKMSNK
                     GSNFITASQPAETIGENLVYALGTLGYDFGTEARRDSFKQLMPWQQIPDSIPIPPNPYD
                     ARQMVDYLAQTPSEAKSLIWTLNLDLTPIYAIEPVGPFANNVYGLLQQLLSGEVQPENS
                     DEYIERVSIPGRLTGKTVKLFSGQVVPVIQPENERGIYGWKINSIINAALEQVPTEQRD
                     SVVRTLQSFLSRIYYDFRNLGTTSQDRAINFAATNLIQTAQTASQAIESGFELDTITVE
                     KSPFCRLDSDCWDIKLKFFDPENSRRARRVFRYTIDVSDIFPVTLGEVKQWSAPY"
     misc_feature    16906..16921
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 25.3"
                     /tool="antismash"
     gene            17022..18244
                     /locus_tag="LC603172_RS06620"
     CDS             join(17022..17502,17502..18244)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611286.1"
                     /locus_tag="LC603172_RS06620"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="ISAzo13-like element ISMae28 family transposase"
                     /protein_id="WP_155120306.1"
                     /ribosomal_slippage=""
                     /transl_table=11
                     /translation="MELTDSLKKLLSETALQLKGAAKRRFMAQTVLELGYGGQTLAAQE
                     LGWNRTTIRKGIKELKRGIICVDNHSAKGRKKAEEHLPFLLENIKSLVDSQSQTDPSFK
                     SQRLYVRLSAAEVRKQLISKYGYSDEDLPSEETIRVKLNNLGYRLKRVAKVLPQKKFPE
                     TEAIFEELANINREADEDPTMLRLSLDAKARVNIGLFDRGGKNRITVETNDHDFNPKTT
                     LTPYGIFIPEFDELFLYFTASTVTSDFIVDILEDFWESEKSRFEKIKTLIINQDNGPEN
                     NSRRTQFMKRIVEFSQKYQVNIRLAYYPPYHSKYNPIERTWAVLENHWNGSILDEIETA
                     LKFAQTMTWKGKHPIVKLITETYEKGVKLTKKAMEKIEEKIERLTESTNQDFPDLGQWF
                     IDIYYDKT"
     gene            complement(18265..18435)
                     /locus_tag="LC603172_RS06625"
     CDS             complement(18265..18435)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012265345.1"
                     /locus_tag="LC603172_RS06625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_221275324.1"
                     /transl_table=11
                     /translation="MKPLYRKTLLRFLPIVFNLERANQLSPLPDKDLADFCPLIEPYQV
                     TKNQESKTVFS"
     misc_feature    complement(18493..18509)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 22.46"
                     /tool="antismash"
     misc_feature    18559..18572
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.84"
                     /tool="antismash"
     gene            complement(18600..18902)
                     /locus_tag="LC603172_RS06630"
     CDS             complement(18600..18902)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012311.5"
                     /locus_tag="LC603172_RS06630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S8 family serine peptidase"
                     /protein_id="WP_221275495.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 4.9e-06, bitscore:
                     24.3, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDITEILGIQELWRKTRGDPRICVAVLDGLVDKSHPCFQGAHLS
                     RLPTLVDGEATSQGDMSKHGTHVSSIIFGQHGSPVEGIAPSCRGLIVPVFGTSRE"
     gene            complement(19533..21155)
                     /locus_tag="LC603172_RS06635"
                     /old_locus_tag="MAN88_12850"
     CDS             complement(19533..21155)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768434.1"
                     /locus_tag="LC603172_RS06635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12850"
                     /product="metallophosphoesterase"
                     /protein_id="WP_239698171.1"
                     /transl_table=11
                     /translation="MRFIFELAQLQQSATEIKQMLDQKIFKIGVLKRAMSKNKIIVLSD
                     IHIGDNSPTVWYQKSFHEPYLTAVLDHVIKNASSIQELILLGDIFDFWTYPPDKRPPSF
                     EQIIEQNPNILGPQGKLSQVLTALEGQVTYVRGNHDMNVTQEDLNKIQNPDYTIKLSPS
                     DIYFPLGEDNKKIVCTHGHLYAMFNAPDTSVKFNPLPVGHFVSRAAAYELQQTLPPGKT
                     VADLTGQTSPNSIDLKSLATTIMAAQSGFSVTDLLLNYITQSTKMPEIQPIILPDGQTT
                     TIAEVKPLYSQLWQQWINNNGGARDGLLVAIKAALADAKDYYMGWFAQKLALECGADLV
                     VMGHTHTPISGLKKGLIQYVNSGFECPSKPDIGKQHVNFIEIDTDSYQGAIFKVVNQQG
                     SYQIEADSAEQTSVIIPGLSQDYSCYVTVENKSPILPIQLVRYEANEGHYIVAPTQFIG
                     PREKGRFWIQDYPGITKGSEGRLIYFTGDREIMLRYSCPVGLSPNSCSGAEFYTSVDGI
                     NWGERNQIVNKGIGHPFFVRFVL"
     gene            complement(21588..21797)
                     /locus_tag="LC603172_RS06640"
                     /old_locus_tag="MAN88_12860"
     CDS             complement(21588..21797)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012265117.1"
                     /locus_tag="LC603172_RS06640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12860"
                     /product="heavy-metal-associated domain-containing protein"
                     /protein_id="WP_061432373.1"
                     /transl_table=11
                     /translation="MTITLKVPSIACEGCANTITKAINNQQPAAQISVDVANKIVTVET
                     TASPAEIAQWISEVGHTVESGGEG"
     gene            complement(22054..22299)
                     /locus_tag="LC603172_RS06645"
                     /old_locus_tag="MAN88_12870"
     CDS             complement(22054..22299)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002794861.1"
                     /locus_tag="LC603172_RS06645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12870"
                     /product="hypothetical protein"
                     /protein_id="WP_002794861.1"
                     /transl_table=11
                     /translation="MIENRQFLTPEESADVDAALLTSPEKFLTRLTISSLRLLKIIAED
                     TGVTLEQLTHKQVIQWLEKDSKLRREQGIEAAVLKW"
     gene            complement(22304..25174)
                     /gene="polA"
                     /locus_tag="LC603172_RS06650"
                     /old_locus_tag="MAN88_12880"
     CDS             complement(22304..25174)
                     /EC_number="2.7.7.7"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /GO_process="GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="polA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002781453.1"
                     /locus_tag="LC603172_RS06650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12880"
                     /product="DNA polymerase I"
                     /protein_id="WP_221275496.1"
                     /transl_table=11
                     /translation="MTSAEQNKLFILIDGHSLAFRAYYAFAKSRSGPLRTSTGIPTSVC
                     FGFLNSLLQLLETQKPSYLAVAFDLAAPSFRHEADVNYKANRQETPEEFRPDLTNLQSL
                     LAAMNIPIVTSPGYEADDVLGTLAKQAGDNGYTVKILTGDRDLFQLVDEEKKTTVLYLD
                     INAVKSTSGQGYTEFNSQAVTEKLGVTPKQVVDFKALCGDKSDNIPGVRGIGEKTAIDL
                     LKKYDNLDDIYTNLESIKGMVKTKLLEGKTAAESSRYLAKIALDAPVSIEEEKFRLRGF
                     DRRLVQPLLARLELKKILQKLDQIQQHLGGTPTLELTSDADSNQLSLFDLPPISSPVLI
                     APEIIDTIPKLDALLAKLTTFTNPHFPVAWDTETTDLNPRVAKLVGIGCCWGKELNSMA
                     YIPIGHQSGDNLNLEIVLEKLRPILAGDNYPKVFQNAKFDIDVFYHQGITVKGLVFDTM
                     VASYVLHPELTHNLEDLCDRYLTGITSLSYKSLGIPAGKTIADLDITTTANYCGLDAYA
                     TYLLREKLQAELTKIPPLYQLFSEVESPLVMVLWQMENYGIKININYLKNFSQQLERDL
                     ADIEKKAYESIGETFNLSSPKQLSEILFDRLALNRKKSRKTKTGYSTDQSVLEKLQGDH
                     PLVDYILEHRTLSKLKSTYVDALPKLAEPKTARVHTDFNQTITSTGRLSSSNPNLQNIP
                     IRSEFSRRIRQAFIPEDGYLLVSADYSQIELRILAHLSQEPVLLEAYRSNQDVHKVTAQ
                     LLFDKREITPEERSLGKTINFGVIYGMGAQKFARESKLTVEQGRKFIEKYHQRYAQVFE
                     YLENSKKQAIAQGYVETILGRRRYFNFDNPLLKRLRGLSLHDIDLDSLKLNNEEAQLLR
                     SAANAPIQGSSADIIKVAMVKLAEVLQNYRARLLLQVHDELVLEVPREEWPSLESKIKT
                     TMEEAVSLTIPLLVEIKAGDNWMETK"
     misc_feature    complement(25208..25226)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 24.14"
                     /tool="antismash"
     misc_feature    25211..25229
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 24.02"
                     /tool="antismash"
     misc_feature    complement(25526..25540)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.22"
                     /tool="antismash"
     gene            25631..25867
                     /locus_tag="LC603172_RS06655"
                     /old_locus_tag="MAN88_12890"
     CDS             25631..25867
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517131.1"
                     /locus_tag="LC603172_RS06655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12890"
                     /product="hypothetical protein"
                     /protein_id="WP_002746188.1"
                     /transl_table=11
                     /translation="MTPHLSLSQIIESIDQLSLEEQNQLFEKLAQKKQQQEHPLLNIPV
                     TLTYNPLLDEVIESIETYRQKVDQEDSQQVETP"
     gene            25864..26292
                     /locus_tag="LC603172_RS06660"
                     /old_locus_tag="MAN88_12900"
     CDS             25864..26292
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002735186.1"
                     /locus_tag="LC603172_RS06660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_12900"
                     /product="type II toxin-antitoxin system VapC family toxin"
                     /protein_id="WP_061433283.1"
                     /transl_table=11
                     /translation="MTQYILDTDHITLLQKLHPNLVRRVASIDPDDTAITIITLEEQVR
                     GWFSVIRKYSQSNQYEKLIKGYQGLRDIVNYLSKFKILNYTLEAHYHFRELREQKIPIG
                     TQDLRIAAISLSENSILVTRNYRDFSQIPNLRLQDWTI"
ORIGIN
        1 tttggccggc gctatcctca ctggcggtat ttgtgggatt tggtttgaat ttatttattt
       61 tgccccgaaa gccttcaccg aagttatggc cgcttattcc ctgctagtcg gcgtttattt
      121 aggatgctac ggcaaaaatc ccccctctcg tcagcgactg tattggacag ggtttttcta
      181 tggattgacc acctatcttc gatttcattt actgcctgcc atagccatcg ccctcattta
      241 tcaagttaga caaaaaaatt ttccacaatt actgcccctt gccctcggtt ttatcggtcc
      301 ggttttgctg gggggtgctt tagacgcttt cacttggtcc acccccttcc aatcgatttg
      361 gaaactgatc tggattaata tttttgaagg aaaatttaat agttttggcg tatccccctg
      421 gcagcaatat tttacttggt tagccctcaa ttggtcttgg tggctgtttc cgatcctcgt
      481 cttctgtgca atcgctgctc gtcgtcactg gattctcgcc cttctggccc tagttattat
      541 cctctctcac agctttttag gtcacaaaga atatcgctat atgttccctg ccctaccact
      601 aattattatt ctcgctggtt tggggactgc cactctcgtc tcgcgtttat cgttaatgcg
      661 aggttccctc gccggcaaaa ctatcgttat ttttagtagt ttacttcttt gggcttttct
      721 ctcttggttg ttggcggcgc gttttaatac cgaccagact tttaatttcg gaccgccttg
      781 gcaaaaaccg ggctatactc actggcaaac ccacggcgga caattacaag cctatcagta
      841 tttaagtagc gagaaaaaac tgtgcggttt aggagtaaaa ggattggcgt ggcagtacac
      901 ggggggatac gcttacttac accaagatgt accgatttat ttccttaaag ataaccaaaa
      961 tttcgaggaa ctacaacctt attttaatta tctattattt aaccaagaca ctgccgcggt
     1021 cggagattat caacgggata aatgctttaa cggaacttgc gtttacaggc gctcgaactc
     1081 ttgtcaagtt aacccgaatt atcatatcaa tcagtatctc aaagaagctg gcgagtagga
     1141 taatatatct ctggcaaact tgctaaattt gtcaaaaata agtctaaaaa gcagagaaaa
     1201 aattttaatt atgtaagcgg gataacttcg atcatgaaaa aagttaaaat cttgccaatt
     1261 tacctaagtt ttctaatttt aatctatata atttctgtca tgacggtcaa cctctcctct
     1321 ttccccggat tgcacggaga tgaggcttgg tttggaatta atgctgggga aatcctccac
     1381 cactattttg agggagcatc tcacctttgc aatgagcata aatacttagt gtaaaaatag
     1441 gcttttcgag ggtaattctt gttttaattc tccatgtacg cagtctttaa aagcctcgat
     1501 ttcgttccaa gacacgatta agagtggctt ttaggctaag tataattact tatcgcgtaa
     1561 gtgagatgct ccctattttg attgaatact gtccccattg tctagatttt cccaaatctt
     1621 ggaaaattat ctaaggaagt taccgcaaat ctatcattat taaaaacaaa tcatccacac
     1681 aaatcctcag cttaattttg ataaaagcca ataataagct gactttaaaa ctcaaaactt
     1741 ttggtaaata tcttgacaaa tgatcccgat cgagggtatt attggcgtgg ttttggtaaa
     1801 aatatccaac agccagagac tccctagact aacttagtaa aattaattcc tccctattga
     1861 tagatgaaaa tcaagaaata tcttaagttc tggaaatatc ctcgcttagt tgatgaacga
     1921 gagactttaa ttgaagaacg agattctctc aaagatcatc tgaagagttt gaatcaagaa
     1981 aaacaagaaa tgattcggga aaaaaatgct ctttatgaaa gattaggtca gttatcctta
     2041 accattcccg aactgcaaga aattagtccg aaacttctcg atattagcgt gatcaaaaat
     2101 aaggcccgcc agtttcgagt ccaattagat gccgaaaaag aaaaactggc tcccgacaat
     2161 tttggctggt atccttacaa cactttaacc tgcttcgaga tcctcgaatt actattaaca
     2221 ggtaaaaacc gttttctact agaattaatc tcggaaaaac cgattgttga tattggttgt
     2281 gccgatggcg atctggcgtt ttttctggaa tctttgggct gtaaagtaca ggtggttgac
     2341 tacgctccta ctaactttaa tctgatgcaa ggagttaaac tgctcaaaac tgctctttct
     2401 tcctctgtgg agattcatga tgttaactta gattcccagt ttctgcttcc cgaaaaaagc
     2461 tatagtttag tcatcttctt aggaatttta taccatctca aaaatcccta ctatgccctc
     2521 gaaaccttag cgaaatctgc caattattgc ctgattagca cccgcgtcac taagtttaat
     2581 acggtgagta ataccgctaa tcgagtcgat ctttccgcga ttcctctagc ctatttactc
     2641 gatgaactag aagcaaataa tgattctacc aactattgga tcttttctaa tgccgggtta
     2701 cgccgaattt tacagcgaac cggctgggaa atttgcgatt atataacagt aggtaatacc
     2761 gaaaactccg atcccgccag tcgtgacggc gatgaaagag cctattgttt agtaaaaagt
     2821 cgtttctatc aagattagaa atgcctaaat atcagaccag tctcctaaaa acatgaacta
     2881 tccccctaat cccctcttaa accgacgtag tttgctgaaa ttagcggccc tagccggagg
     2941 aagtgccgga ctagctaccc ttctctcctc tcggctcagt tattctgccg accaaaacca
     3001 agctagtgcc gagaatatag aggcaataga ggaatttttg cggcaaattc gcccagtaaa
     3061 ttcgcaaaat ctccagtgga cagaatatag ctttgaaaca gtcaccgttg atcgtcaagg
     3121 gcaaattatc gatcgccgtc agggtagcgc tcgctcctgg cgcgaacctt taggcaacgg
     3181 cagcgaacta gaaatggtgg ctattccggg gggcagtttt ctacgaggct cgaaaaatga
     3241 agaaagaggc gatcatacta gcagtgaaaa accagaaaat tccctgacga cggcggcttt
     3301 ttttctgagc aaatacccga tcacccaagc acaatggcga gcgatcgcca aactggcgaa
     3361 aattaatctc gaactcaacc cggcgccctc ctttttccaa ggcgataact tacccgtaga
     3421 gagagtctcc tggtacgagg cgatcgaatt ttgtcaacga ctctcccgag ccaccggcaa
     3481 agattaccga ctagcgagtg aatcacgctg ggaatacgcc tgtcgcgccg gtacaaccac
     3541 ccccttttac tgcggggaaa ccctcaccag cgagcgggcc aattactttg ccgtctatga
     3601 tccctacgcg gaagaaccag ccagcgccta tctggccagg actaccccgg tgggcagttt
     3661 cgcccccaat aatttcggtc tctacgatat gcacggcaac gtgggcgaat ggtgcgccga
     3721 tagctggcac cgcaattacg agggaacccc gcccctggat ggtagtgctt ggttagacag
     3781 ggatactcgc ttgcagcccc gttatcgttc cgcccgttta gtgcgagggg gcagttgggg
     3841 cgatgaagct aggcacctac gcagtgccaa tcgcaccttt tgccttcccg atcaacacca
     3901 cagtgtcttg ggtttacgag tcatggccga tagtaccggt taatcaatgc ttgtgtccgc
     3961 ccaagagacg acaaaggtat ggttcaatag gttaggttgc caaaagcatt cagaatcttc
     4021 tgccgttgtc agtgcgtctg tagctaaatc aaccgcgagt gaggtcaaat ttttcatgaa
     4081 gatacagcag tctcatcttt tcttaactcc ccccacagga gatctacagg tacctttatc
     4141 tcctcctccc ccggacgacg gcaatttaga agaagaaaaa aattattctc tcagcctgtc
     4201 tctagtcatt cccacctaca atgaacggca aaatatcgtt caattagtca acattctcag
     4261 tcaaatactg gacgaagccc ttcccaacga ttacgaactg atcgttgtcg atgataatag
     4321 tcccgatcgc acttgggaag tcgccgcgtc tttaatccct gaatatcccc agctgcgagt
     4381 gctgcgccgg gagggagaaa aaggcctatc ctcggcagtg gtacgggggt ggcaagtggc
     4441 acggggcgac atacttgggg tcatcgatgc cgatttacag catcccccgg aagtgttatt
     4501 aaaattattc gagcaaacca gagccggagc cgatctctcc ctggctagtc gccatgtgga
     4561 agggggtgga gtcagccaat ggagtctgac tcgacgcttc ctctcccggg gggcgcagct
     4621 gttgggatta gtcattctgc ctaacgtggt gggacgggta tcggatccga tgagtggcta
     4681 tttcatggtt aatcgacaag cgatcgcggg accgatctta aacccgatgg gctataaaat
     4741 tctcctcgaa gttatcggcc gcggcaatat cgaccggatc gccgaagtgg gttacgtttt
     4801 ccaagagcgt caagacggtg aaagcaaagt cacctggcaa caatacatcg aatatatcct
     4861 gcacttgctg aaattgcgtt cccgcggtcg tttcggtggc ctgaaaaggc gttggcaatt
     4921 ccccctcgct cgctttcttc gctttagttt agtcgggttt agtggcgtat tcgtcgatat
     4981 ggctatcttt tatctcctca gcgatcccac taccctcgct tggggagtaa ctcgcagtaa
     5041 gattatcgcc gccgaggtgg ccattattaa taattttctc tggaacgatc gctggacttt
     5101 tggcgatttg tccaccggcc aaaatcagtg gcagcagcgt tgcaaacgtt ttcttaaatt
     5161 taatcttatt tgtttggccg gtttgatcct caacgtcctg ctcttaaact ttttcttcaa
     5221 tgtcttacat attaatcgct atattgctaa cttaatagcg atcgctatag ttaccgtttg
     5281 gaacttctgg atgaatttaa aactcagttg gcgtgtgacg gaggttaaac ccgaaaagtc
     5341 cggcaagcca taggaaaacc agggaaaact tggcctctag tgccgcaaac cctttttaac
     5401 tctcgttcaa cctctggcag cattaattct cgggacttaa aagcggccca gggtcaacag
     5461 gaaaccgatt actggagatt aagtctcaaa gaggggatgg aatggcttaa tcaaaatgcc
     5521 gccgctaatt ctaccattgt cgtcgctgac ccaatctttg cggcggaaat ggtacaagac
     5581 taccaaaaaa acttgacgat gatcgaaatg actgctattc cgccgatttc cctgcaaaac
     5641 taccgtatcg ctgcgattat tccctgtcac aacgaagaac taacgatcgc taaagtggtc
     5701 tcccaatttc aaacttttct gccagaagcg gcaatttatg tctataacaa ccgctctagc
     5761 gatgataccg tgaccgaggc cctcaaagcg ggggcgatcg tgcgtcagga aatccaaccg
     5821 ggaaaaggca atgttgtccg gcggatgttt gccgatatcg aagcggatat ctatattctc
     5881 atcgatggtg atgataccta cgaaattgcg gcagtacgac gcttaattga acgaatgctg
     5941 cgggaacagt tagatatggt ggtcggtgcg cgtcgggaag cggcaaaatc ctcccaggct
     6001 tatcgtcccg gtcatcgcgc cggtaatcta tttttaacag gattggtgaa atttctgttt
     6061 ggagcgcgtt taatcgatat gctctccggt tatcgcgtct tttctcgtcg tttcgtcaaa
     6121 tcttttcccg ctttgtccaa tggtttcgag attgaaaccg agttaactat ccacgcttta
     6181 gaattaaaaa ttcccttcgc tgaagaacca accctctacg gggaacgtcc agaaggttcc
     6241 caaagtaagt taaaaacttt tcaagatggc tggcgggtat tgggaaccgc tatcctacta
     6301 tttaaagaaa ttcgtccttt tctctttttt agccttgtct cgctgatttt ggcggttatt
     6361 tccctcctct tagcgatccc cgtcctgttt gaatacctcg aaactggctt agtaccacgt
     6421 tttcccaccg ctattttatc ggcctcgatc atgcttttag cctttttaag tttgacctgt
     6481 ggcatgatcc tcgattccgt ctcccgggga cgaaaagaga tgaaacgaat ggcttatcta
     6541 gctaacagtt ggctgaaagg ttagggaaaa agttcactaa acatacaggt ctaggtaaca
     6601 tctggggtta aaccgtctct aactgttgca gcacggagga gggttttttg tcaataaatt
     6661 ccgccggcga aatttgatat ttaatcagat tggctaattc ctgtagttgg ctgatggctt
     6721 ccgcaccttc gagtttcatt aactctcgat catcgcgcat ttctgtccag gtgatgccgt
     6781 aatcggacac gagaaaccga attagatggg tttcacccaa tccgaccata aaggatgcgc
     6841 tgttcatttg accgccacag gtgtagcaag atgctagata accttgattt tctagtgcga
     6901 tcgctaaggc ctgtaaatcc atgactaagt ctttcaccag ttggcgatgc tgttctgcaa
     6961 gtcgtttaaa cacaatgttc ctcctgacaa cactcctcaa ttataaaacc ttaatacttt
     7021 tttaagattc ttttttgaga aaataagata tgtgaaccag gtttttttac ggatgttaag
     7081 taacaaaatg taaagaaccc gatacctaga gtagcgtgaa ttcctgatat cgccaagtat
     7141 atgtaagtac aaagctacca gtttatccgt ctttctaccc cctcgatccc aactattccc
     7201 atcatgccta tttttaaagt ttttgacatc ctcgccgccg taaaacggac ggtgattccc
     7261 aaacctcacg atttaggttt ctgcttcttt cccttgcggg gttccgcacc tgccttaaca
     7321 gatttactct gttctggtct tatggtcgct ctacagactg acaccgcaag ccctgcggcc
     7381 aaaatatttt tacttgcgtt aatatctcgg tcatggtgag tcccacagtc tggacaatcc
     7441 cattctcgaa catttaacgg cattttctca gcaatatgcc cacaattact acacctttta
     7501 gagctaggaa accatctatc tatttcgatg taatttctcc cataccaacg gcatttgtag
     7561 gctaattgtc tagtgatttc tccccaacta acgtcagata ttgcctgaga taattggctc
     7621 ttcggtttgt gttatgcaat ggaggggagt tataagggat ggaaccctta tatagaaagg
     7681 catttagcta tttttgtcaa ttgtttttta tctagagcga actaatcaat taagtctctt
     7741 gccagataag gatttagtcg atttatgccc ccctatcgaa ccataacaag taacgaagag
     7801 ccgatcttat taagttcagt aatatttcta ctgtccgacc cattgattcg tctgcaatcc
     7861 cttttggctc gatggtttgc agtatctctt ctcctgattt cttgctcgaa tctagtcttt
     7921 gcgtcatgtg tcatattcta ttatattgcg tgtcccttgc ttaccttttt tctctactac
     7981 ccctacttat tgagcggata cgatcgcttt tacttttgag attctgatga gagaatgaaa
     8041 cagccctact ttttaagggg gattaaactt ctgtagtcag actctggaga attggtataa
     8101 cgaagaacct tgaattggtc ctcttccaat atctcgtaaa agcctataat cactattcat
     8161 ttgctcggca gttagccata aataggaaaa agtattttgt agtttgaacc aatttttcaa
     8221 atcattaaat tgaaattcaa tgtccatctt tgctatctcc aattcagaaa aaccaaaaga
     8281 gccatcagga cattgttgat tgttaataaa agcaaaagac ttttgaatgc catacttcgt
     8341 agagacattc aaataattaa gcatacgaaa taatcttaga gctattttaa gattgtaatt
     8401 ttgtaagtat tcaaataaaa ttacttctaa gacatcaatt aatccatgat atattggagt
     8461 tcttactata ccataaacag ttaaattttc taattcagta atactcttct gaattgctaa
     8521 atcatctgcg tttaataaag ctaataaatc tggcttgtaa ttcatcacct taaatgaagg
     8581 ttttggtatt aagtttaaag tgccagcgag aaaaagaata ggcctttcgt tcagcgagtt
     8641 atcacttgat ttctctggca atttatcaat gacttttttt acatggtcaa caatgtttgg
     8701 aagctgagtt cccaataatc tgtaggtaga aatagcagca agcgataaaa gtatgagatt
     8761 actgctgcga aattgatgtt ctgataaaag gattaatttt gctgttagat ttttaactat
     8821 cttaattgac tcattgagaa tttttggctc aatatcgtga caaattacta tcgctatata
     8881 ggtttgtaaa agcgaattta tatcagagtc aggcagatga ttaatacgag taacaagcca
     8941 atgaaagtaa gcttctatat taattttatt actttcatca aaaggagata atgccatcat
     9001 aaataattcc ctccatagag atagaacata gaggctaaaa atgttactaa agtaggatga
     9061 taattttttc taaaagtatc tttttcaaga tttacttctt ccgaattgct ttcttgtttt
     9121 tcggacaatc tactttttgc tggaataaaa ccttgaggat tttgtatctc accaaggctt
     9181 ttccctgcta attggattaa aggatgaaag tagtcacatt gcaaaaaatt acaacatatt
     9241 aacagctcgg ccactagatc ccaatctttt ttcttcgtat ataatcccag tataagtatt
     9301 atgtattggt agatttcatt gtatcgctct agtaatattg gggctaatgg ttgctgtcca
     9361 aaatcgctta agtaaaagat aatgtgtgtc aagttataag catcagtaat acgcaaatgg
     9421 attggagatg gaaggtgaaa agcactggag tatttgtaga tgctactaca atcagaaagg
     9481 cgatgcttaa agccaccgag cttgaaataa tagctgagtt ccatttggct gtaaggagtt
     9541 ctctctacgg catcgatgaa atcttcatca atcatctttt gtagaaggat ttttaattgt
     9601 tctacctcta tagttgtttt tagatcgcaa gcttttaggc atgagtatat agataaatag
     9661 aattcaaaca ggccaaacct acgcgtcatg ttaaaagtaa agttactttt ctctagttct
     9721 tctacaatat gtccaattaa aactttaaat tcctggcgtt gtcgaacttg tgggtagcga
     9781 ttcatcacca gacaagctat ggccagctcg ccaaaagctt tatgttcaat agtagctttg
     9841 acttccctat cgctcgactt atggttgggc aggctaaagt agtcaatatg ttgagtaatc
     9901 cagtctagtg tcctttctag gagagaggga agtagtatat cgaagtaatt aagcattctt
     9961 acccgcctta tcttgagcta tcccgcctgc aattttctaa ttagttggga gaatcccacc
    10021 aagttttcgc ttcgcccccc aaagtaacag ggaagatgtc agatacatca agggtatagc
    10081 ggtacacttt ccttgcccta tcattatttt ctggatcaaa gaactttaat gctacatccc
    10141 aacaatcact attgagccgg cagtagggac ttttctctac tgtaatgctg tccagttcca
    10201 tccccaggag aatcgattct gaaaagaccg agaccgattg aacaatattg gtagcggcaa
    10261 agttgagggc gcgatcactg gcagtctgtc ccaaattgct gaagtcgtaa tagatacggt
    10321 taaggaagtt agagaggact gccgctagtt gtggttgttc gtttactggt actgcttcta
    10381 gagcggcatc gatcaagctg ttaactttcc agccataaat tccccgttct ccatcaggtc
    10441 gaattacggg aacttcctgt cccgataaca acttaaccgt cttacccgtt aatcgggcgg
    10501 ggatgctcac ccgatcaata tattcactgc tgttttccgg ctgcacctca ccctgtaaaa
    10561 gtccttgcaa aagctcatac acagcatagg caaaaggtcc gaccggttca atggcataaa
    10621 tgggggttaa ctccatgttt aacgtccaaa ttaaagattt agcctcagat ggattttgag
    10681 ccaaataatc cgccatctga cgggcatcat aagggttagc gagaacgggc ggggttcctg
    10741 ctggatagcc agggattgat tgtcggggca ttagttgttt gaaggagtct cgccgcgctt
    10801 cagtaccaaa gtcatatccc aaagttccta aagcataaac caagttggtt ctagcactag
    10861 gagtgatact atcagcagtc tggctagggg taatggcagg tgagctatcg gtgggcatga
    10921 cagaatcttc taaaattgat gagatcgggt ctggttgagc aattgagtct gtgactgcca
    10981 ttgcttcgat tttccaggcg gtctcaaaac cactggatgc cacgatgagc aaaggctttt
    11041 tgtccactaa attgattgca ctgggaacgt ttaatcggcc tagcaaacag cgttcaactt
    11101 cttcaacctc ttccggatcg caagggatag cactgtttaa aatagcagcg cggacagctt
    11161 cggcatcagg ctcttgacct tgttgtagct gtaggctcat gagcaaagcg gcaactccgg
    11221 tcacaatggg agcagcacaa ctggtcccct tcttgcggag cggtttatct gaccatggct
    11281 gcgctcctag tatattttcc cctggggcta gcaccccttg gttttgatat tcccctcccc
    11341 agttactaaa cttgaagggc tgtccgttgt ctttcatcgc tccaactgtg agaacgtggg
    11401 gcagtactgc tggaatacac caacattccc cttcattgtt gcctccgggg gcaacgagca
    11461 ggatgttgtt atcttggcac tgacgcacag ctcgttctag caggtcatgg gctacgccag
    11521 tttgggtggg aatgcagaca gcaatatgga tgatatttgc ccccgcattg agggctaggt
    11581 tgatagcgcg agagaggttg agcggagagc taaagttgtc gaagtcgtag ccaaggggga
    11641 tgttgatccc gcgacagtta ggtgccagtc ccttaaccgg tgaatcatgc tggccaaaga
    11701 tggtactggc aatatgggtg gcgtgggaga caaaattgag atagtcgcca aagggagcag
    11761 gtatggcaga ttccattttg gctgcttttt cctcactatc ttcttctctg ttttctaaat
    11821 ctatatagag gtcaatatat tcttggggta cttccagcat ttcttgccaa taaggtttaa
    11881 tttgagcgat attggctttc tggaaacagg cgcgatcaag gtcggcggga ccgtcaagta
    11941 cggcaacggt aatttgagga ctgcctagag tgcgagtcca tagttcggta attccaggta
    12001 tatcaattat atccggcata gtttttcaca aatcttcaaa aaagttgagc aatatatcaa
    12061 aattctgttc ataagttaga gaattggctt ggcttatcca gataatcagt tcatacagta
    12121 atacatattt aatctttgaa ttgacctagc tgttaattcc tgttcgggga tgcacactac
    12181 tagacttctc accggttgat gccggtaata agcatgaacc ctgcttgcca tttcattgcc
    12241 caagttatca ataaagctgt tagggttgag ggtatgataa tacataattt tgctatcatt
    12301 agccctgctc acaccaatct ggatagcttg acaatcattg actagacgca aagcaggtgc
    12361 acagacaact ttggctaacc tttcccaaac ttgtgtttgc tgaaattcct ctgatgataa
    12421 agaaatataa agctcgatag cagtacgacc atcaaaataa aaatcaaaac ctaccaaaaa
    12481 ttcttgattg acgatcaatg ttgacagctc tttcttgtcg tcgcaccaag ccatagctgt
    12541 tgccatcttt tcggggtagt cattcatcca aatatagagc ttcacgcgcg aatcagctaa
    12601 ttccctgcgc gcatctactc ccaccaaatt tctgattact ttactaaagt caaagtcagc
    12661 tcctaaaaaa tgctggagtt gctgatagtt gagcttcacc tcagtccgac cttccacttg
    12721 atgaaagaaa tcaatcgttt ccctaatttg cttttcagcg tattggttat tatacaatgc
    12781 caagctgaaa cgagcaggat aaagctcatc cccatcaatt ttacaagcac agtcaatcga
    12841 acaatcagtt tgtgactgca taaatctctc gaaaagttct aaaggataaa gagcttcgat
    12901 ttcaaagata ttttcatgcg ccttcatata cattagattt ttgctcttat gcaactgggc
    12961 attagcgtag ttaatcattg ctttttaagt tcttgctccg aatagtcttg actatttact
    13021 tgacagaaaa ctaccttgac ctctatgaca aacctacttg acatgctcac cgccgtaaac
    13081 cgagggtgat ttctcaccac gctacctttt tagagtagtc gccgaatggt tgacgcttca
    13141 ccaaaaaacg cttaattgct taagtctgac actttctcca cgtcaattta aagtctccga
    13201 atgccctccg gcgactaatt taatttgggt ttatacatac cgacacaaga tatgtttcgc
    13261 ctctagcctg gagttcccag cggccgtcag cttcccctta ctcatcgtca acgtctcgac
    13321 gctgatcgac gttaacccct cgacgtagct cagggtttac cctgagcgaa gctgaagggt
    13381 caacccgttg ccagaatttc aacctaatat agcgtttttc agtctgctga ggtacaaaag
    13441 ttatgggttt taggcaaaag gcaagaggca aaagggaaga aaaataggtg tacctcacta
    13501 gcttaggaaa cgctatgtat ctatttcgga gtatctatga tagatagggc aggtttttta
    13561 gaccagtgag actttaacag aaagcccccc taaaagaggc ggggttttta acccaaattt
    13621 tcgataagag aggtgaaatg aaaaatgggt gagtcaataa acttatcttc atcacttcta
    13681 ctctcatctc ctctcttatt cttctgtttg ctagtgagtt ttactcagct ccatcggcgg
    13741 caaagggagt tgaccagttg ttatcgccat tcagccaagc ggcattgacc ccagcaaagg
    13801 gagttgacca gttgttatcg ccattcagcc aagcggcatt gaccccagca aagggagttg
    13861 accagttgtt atcgccattc agccaagcgg catccacaga ggaagcctgt tttaaatcgg
    13921 aagaggtcac cgcggggcgt tcaactggag ctgttaattt aggaagcaaa gtcggatttt
    13981 tcattgtttt ttttcctagt tcttggtagg ttacgaactg aagattagcc taagcttttc
    14041 tagctgaaat atacatcaca aatgctagat atgtcaactt actttatatt acttaaaacc
    14101 aatcaacagc actgcaaaaa ataagtttct gtactttact tcacagtaaa ctaaactgat
    14161 ttattcagct tagaaataga aagtatttct cgaaatcgct ctactttcac cacgggagtg
    14221 tactcaattt tatgagtcct taaacccctc ttttggttaa gatttgagac ttaaattact
    14281 gaattaagat tatagggcat catctgaggc aaaagggatg ccctgcagca cacctcctaa
    14341 tccaggaata tatatcgctg tctcaacccc acccagttcc ggcattggcg aagaagggac
    14401 atttatagat gggcgtttaa ttgactcagt tagttgaggc aataaagttt tctttttcat
    14461 ttgattcatt ttccagagta aaaaagtcaa ttccaaatgc gtcctcgacg ccactcctct
    14521 gtttctatcg tttttcgccc tagtcgggtt tgatcaccca attcttgttt gagcaggaat
    14581 ttgacatcct ttgaaccaac agaggcaagg ggtggatttg cctttgactc taagttatca
    14641 ctcataaaag ggtccttcca gtttttctaa tcggttgaca aattgacccg tgacattagc
    14701 tttatcgctt caagaagacg attgctgtcc gtcttacttc agcaaagagc cacgcaaccc
    14761 agaagacttg atttgctggt agctttttga ctgaaagtaa ggaggatcgt tatagttagc
    14821 gccgtgaagc aaacgtatcc tgataacaac cagattatta atggaagttg gatcgctgat
    14881 acgggaacca tcattatgaa ctacatcgac cgccagatgt ggggctataa catctggtct
    14941 ttttaccggt tttgcttgta tggggatcat cttgaactta accttcaact gattcttcgt
    15001 tacttggtat ggttcgattg gggggcataa atcgactaaa tccttatcta gcaatagatt
    15061 taattgatta gttcgctcta gcccgaagac aattgacaaa aatcgcccaa tgtcttcccg
    15121 tataggagtt tcatttctta tcatcctgta cgtcgcacag ggcagaccga agagccattt
    15181 tagtaaggtg cagaccattg tttgacttca cccaaagtaa ccgggaagat gtcgcttaca
    15241 tcaattgtgt atcggaacac ccttctggct cggcggctat tttctgggtc aaaaaacttc
    15301 agtttgatat cccaacaatc actatctagt cggcagaagg ggcttttctc tacggtaatg
    15361 gtgtccagtt caaacccgct ctctatcgcc tgagatgcag tctgagcagt ttgaattaga
    15421 ttagtagctg caaaattgat ggctcggtct tgggaggttg ttcctaaatt tctgaagtca
    15481 taataaatcc gtgagaggaa actttggaga gttctcacta cagaatctct ttgctccgta
    15541 ggtacttgtt cgagagccgc gttgatgata gagtttattt tccagccata aattccccgc
    15601 tcattctcag gttggatcac aggaacaacc tgtcccgaaa acaacttaac cgtcttgccc
    15661 gtcaatcggc caggaatact cacccgttca atatactcat cgctgttttc tggctgtacc
    15721 tccccgctca agagttgctg taaaagccca taaacattgt tagcaaaagg accaaccggt
    15781 tcgatggcat aaatgggggt taagtccaag tttaatgtcc aaattaagga cttggcctcg
    15841 gagggagttt gagccaaata atccaccatc tggcgggcat cataagggtt gggaggaatg
    15901 ggaatggaat cggggatttg ctgccaaggc atgagttgct tgaaggagtc ccgccgcgct
    15961 tcggtgccaa aatcatatcc caaagttcct agtgcgtaaa ctaagttctc tccgatggtt
    16021 tcagccggtt ggctagcggt aataaaattg gaacccttgt ttgacatttt agatacgtta
    16081 gatgttaaag aattctgtga gctagatgga cttatgactg aggctgctaa agctgtctca
    16141 gggatggaat gacttatttc tgaagtatta gcactagctt caatctcgtt agtattagca
    16201 acatcagctt caatgttgtt gactacatca caaccacagc ctgcgggtgc gatggtttct
    16261 cttagctcag acattgttcc tccttgtagt aattcaattg caccagatat attgattttc
    16321 ccaaccaagc aatgataatt gctttttatt tgattgggat cgcagggcaa ggcactgttt
    16381 aaaatagcat ctcgaacctt gtgaggatcg ggaatttctc cttgctgaag ttgtaggctc
    16441 aacagaaggg cagcgactcc agaaacaata ggggtagcaa aactggtccc gcttaacctg
    16501 acaactcctc ctcctggctt ggcacctaaa atattgtccc cgggggcaag aatgccttgt
    16561 gtttgatagt tttttcccca gttgctgaac tccattggct tgcctaggtt atccattgcc
    16621 cccactgcta gaacagaagg aatggctgct ggaacgtgca agcaaggaca tccatcgtta
    16681 cctgttgctg caacgattaa aatattgttt ttttcacaca actgagtgac tctttgcaaa
    16741 aagtcttccg cttccccaaa atcagtgagt tgacctccac ttatatttat aatgtgagct
    16801 cctgaactaa ctgcttgctc aatagcccgg gacaaatcaa gctgagacag cctttgaccc
    16861 cgatccgcaa agggacttcc aaaaaataaa ctattcatgc tcaagaataa taatcattct
    16921 cggagtgtag gaggtagggg tcgatggttg cctcctcctt ccaattttgt agtagaatta
    16981 atcctgtaca ttattaatca agtttttctt tgtaagtgta tatggaatta actgattccc
    17041 tcaagaaatt gctcagtgaa actgcacttc aattaaaagg tgcagctaaa agaagattca
    17101 tggcccaaac agtcttagaa ttaggctatg ggggacaaac ccttgctgca caggagttag
    17161 gctggaatcg aactactatt cgtaaaggaa ttaaagaact aaaaagaggt attatttgtg
    17221 ttgataatca ttcagctaag gggcggaaaa aagcagaaga acatttacct tttctattgg
    17281 aaaacatcaa aagtttagtg gattctcaaa gccaaactga cccaagtttt aaaagccaaa
    17341 ggctttatgt gagactgagt gcggccgaag tccgaaagca attaatctct aaatatgggt
    17401 acagtgatga ggatttaccg agcgaggaaa ctattcgggt taaattaaat aacttaggtt
    17461 atcgtctgaa aagagtcgct aaagttttac ctcaaaaaaa attccagaaa ccgaggcaat
    17521 ctttgaggaa ttagctaaca ttaatcggga agcggatgaa gaccctacga tgttacgtct
    17581 tagtttggat gccaaagccc gtgttaatat tggactattt gatcgaggag gtaagaatag
    17641 aataactgtc gaaacaaacg atcatgattt taatccgaaa acaaccctaa ccccttacgg
    17701 aatatttatt ccagaatttg atgagttgtt tttgtatttc actgcctcca cagtcaccag
    17761 tgactttatt gttgatatat tagaagattt ctgggagtcg gaaaaatctc gttttgagaa
    17821 aattaaaact ttgataatta atcaagataa tggaccagaa aataattcga gacgaactca
    17881 gttcatgaaa cgtatagttg agttttccca aaaatatcaa gttaatatac gtttagctta
    17941 ctatccccct taccatagta aatataatcc tattgaacga acctgggctg tgttagaaaa
    18001 ccattggaat gggagtattt tagatgaaat cgaaacggct ttgaaattcg cccaaactat
    18061 gacttggaaa ggaaaacacc cgattgttaa gttgattact gaaacttatg aaaaaggagt
    18121 aaagcttact aaaaaagcca tggaaaaaat cgaagaaaaa atcgaacgtc tcacagaatc
    18181 aacgaatcaa gactttcccg atttgggaca atggtttatt gatatctatt atgataagac
    18241 ctagtttgta gttaactaag atggttagct aaaaactgtc tttgattctt ggttcttcgt
    18301 tacttggtat ggttcgatca gggggcagaa atcggctaaa tccttatctg gcaagggact
    18361 taattgatta gctcgttcta gattgaaaac aattggcaaa aatcgaagca atgtctttct
    18421 atataagggt ttcacccctt ataactcccg tccattgcat aacacaaacc gaagaaccat
    18481 aagagtttgt gctgcctatt ttttcggaaa aataggttgt aatgggagac tctttgtttt
    18541 cctagacaaa aataattact ttttcaaaaa aacacttttc tatttttttg ttgggtattt
    18601 tattctctgg aagtcccaaa gacaggaacg atcaacccac ggcacgaagg agcaatgcct
    18661 tcaactggag aaccatgttg gccaaaaatt atactgctaa catgagtccc atgctttgac
    18721 atatccccct gtgaagttgc ttcgccatct actaaagttg gcagacggct tagatgcgct
    18781 ccttgaaaac agggatgtga cttgtcaacc agtccatcca aaaccgctac gcaaatgcga
    18841 gggtcccctc tggttttcct ccatagctct tgtatcccca gtatttccgt tatgtctgac
    18901 attcatgttg aatatgagat attctcatta cagttttatc tcaattagga gtgtatctca
    18961 aaaaaactct tgccgagcat ttaaggacaa aattgtaact attgttaaca attggggagc
    19021 ttcgttactt tttatactaa atctggttat agcgtttcct gttcgcacga ggtacattat
    19081 tgatggggaa aagcttaatc agcaaaggtt taactgtgcc acacaggtat gataatggga
    19141 cttaggcgtt ttcggtcagc aaaaaaggct caaaccatta cgggacaaag ggttaacctc
    19201 gattgatgat tttccttgat ctatctgagt ttcagcgatt ttgggcttct cgaagtaaat
    19261 cccaagcggg gattggagtt gaggcttttc tcgatttgtt ttttgtctaa gtcacaataa
    19321 ctgctatatt aaaaactgat tatttattcc cctttgcctt ttgcctttcc tcataactag
    19381 cctgtactca acggatttag tattattcca cattccgcac cctcaactgt catataaggt
    19441 aatctactcc cgttaagccg atgcttgaac gggagctttc tgtaaccctg aatctatcgg
    19501 acaagccaat aaattcggat ttctgggctg gattacagaa caaaccgcac aaaaaatgga
    19561 tgaccgatgc ccttattgac tatttgattg cgttctcccc aattaatgcc atcaacagaa
    19621 gtataaaatt cagctcctga acagctattt ggactcagcc caacaggaca ggagtatcta
    19681 agcataattt cacggtctcc tgtaaaataa atcagccgcc cctctgaacc cttggtaatc
    19741 cctggataat cttgtatcca gaaccgtcct ttttcacgag gacctatgaa ttgggtagga
    19801 gcaacaatat aatgaccttc gttggcttca taacgtacca gctgtattgg taagataggg
    19861 cttttatttt cgacagtgac ataacaggaa taatcttgag acaatccggg tattattact
    19921 gatgtctgtt ctgcactatc agcttcaatc tgataagaac cctgttggtt aacgactttg
    19981 aatatcgccc cttgatagct atcggtatca atctcaatga aattaacatg ctgtttgccg
    20041 atatcaggtt ttgaaggaca ctcaaatccg ctgttgacat attgaattaa accctttttc
    20101 agcccagaga tcggcgtgtg tgtatgtccc atgactacta gatctgcccc gcattcgagg
    20161 gcaagttttt gcgcaaacca ccccatatag tagtctttag catcagcaag agcagcttta
    20221 atagctacta gtagcccgtc tcgagctcca ccattgttat ttatccattg ctgccacaac
    20281 tgactataaa gtggcttgac ttcagctatc gtggtcgttt gaccatcagg cagtattatg
    20341 ggctgaattt ccggcatttt agtagattgg gtgatatagt ttagcagcag atcggttaca
    20401 gaaaacccag attgtgctgc catgatagtt gtcgccaagc tttttaggtc gatactatta
    20461 ggagaggttt gacccgttaa atcagcaaca gttttacccg gtgggagagt ctgctggagt
    20521 tcgtaagcag cggcacggct aacaaaatgt ccgaccggta aaggattgaa cttgacacta
    20581 gtatccggcg cgttgaacat cgcgtacaga tgtccatggg tacaaacaat tttcttattg
    20641 tcctctccca gagggaagta aatatcgctg ggcgataatt tgattgtgta gtcaggattt
    20701 tgaattttgt ttaagtcctc ttgggtaacg ttcatgtcgt gattaccgcg aacataggta
    20761 acttgtccct caagtgcggt taaaacttga cttagctttc cctgcggacc taaaatattt
    20821 gggttctgtt cgatgatttg ctcaaagctt ggtgggcgct tatcaggggg ataagtccag
    20881 aaatcaaata tgtcacccaa aagaattagt tcttgaatgg agctggcatt tttgataacg
    20941 tgatctaaga ctgctgttaa gtaaggctcg tgaaagcttt tttgatacca aacagtggga
    21001 ctgttgtctc caatgtgaat atcactcaaa actataattt tattttttga catggctctt
    21061 ttcaggactc ctattttgaa tattttttga tccaacatct gttttatttc tgtagcagac
    21121 tgttgtaact gcgccaattc aaaaatgaaa cgcatcaacc ttaagagttg acaaagaaag
    21181 gctgctcagg tatgctgatc gccggtgtga tcctggccat catgctcgcc atcaacctta
    21241 agagttgacc aaggaaggct tatcaggtac tgtctgatca ctaataaaaa caaatcggac
    21301 ccaaaaacgg gacagcaaca tcttaacata gaacaaagaa aattgctata tcaacttagt
    21361 caggagggaa atttatctgg ctcttcggta attgttatgc aatggacggg ggttataagg
    21421 gatggaacac ttatatagaa aggcatttag cgatttttgt caattgtttt tgatctagag
    21481 cgaactaatc aattaagtct cttaccagat aaggatttag ccgatttatg cccccctatc
    21541 gaaccatacc aagtaacgaa gaaccctatt tttaacgggg gaggatgtca accctcacct
    21601 ccagattcaa cagtatggcc gacttcgctg atccattggg caatttctgc gggggacgcg
    21661 gtggtttcta ccgtgacgat tttattggct acatccaccg atatttgggc tgccggttgc
    21721 tgattgttaa tcgctttagt aatggtatta gcgcaacctt cacaggcaat actaggcact
    21781 tttagggtaa ttgtcattgt tgtaactcca gtaacttata agtagtcgtg caaaattaat
    21841 ttcctagtcg agacaggaga ctccgagaca ggagactccg agacgggaga cgggatacag
    21901 ctattaggga tcggatttga gttttcagtt cactgtttcc tcacatcaga agtctgacgg
    21961 agtagtggct tagatgtgta attaattgtg cttagatact tagctaaaaa ctagcataaa
    22021 ttgtcagggg tagctgattt tttaagagtt tatttaccat ttcagcactg ccgcttcgat
    22081 cccctgttcc cgacgcagtt tactatcttt ttctagccat tgaatgacct gtttatgggt
    22141 tagttgttct agggttactc ccgtatcttc ggcgattatt tttagtaagc gtagggaaga
    22201 aatcgttaaa cgagtcagga atttttcggg agaagttaag agggcagcgt ccacatctgc
    22261 ggattcttct ggggttaaaa attgtctgtt ttcgatcata gttttatttg gtttccatcc
    22321 aattgtcacc ggctttaatt tctaccaaga gagggatcgt caaggagaca gcttcttcca
    22381 tagtcgtctt gatttttgac tctagggatg gccattcctc cctcggtact tccaagacta
    22441 attcatcatg gacttgcagc agtaatctcg ctcgataatt ttgcagtacc tcggccaatt
    22501 ttaccatcgc tactttaata atatcagcac tggaaccctg aatgggcgca ttagcggccg
    22561 atcttaataa ttgtgcttcc tcgttgttga gtttgaggct atctaaatcg atgtcatgta
    22621 aggacagtcc ccgcagtctt tttaagaggg gattatcgaa gttaaaataa cgtcttctgc
    22681 ccaaaattgt ttccacatat ccctgagcga tcgcttgttt tttactattc tctaaatact
    22741 caaatacttg ggcataacgt tgatgatatt tttcaataaa ttttctgccc tgttctacgg
    22801 ttaatttcga ttcacgggcg aatttttgcg ctcccatgcc ataaataact ccgaagttaa
    22861 ttgtttttcc taaactgcgt tcttctggcg taatttccct tttatcaaag agcaattgag
    22921 cggtgacttt atgaacatct tgattactac gatacgcttc taataaaact ggttcttgac
    22981 tgagatgagc cagaattctt aactcgattt gtgagtagtc cgctgacacc aataagtaac
    23041 catcttcggg gataaaagct tgacgaattc gcctggaaaa ttcactgcga atagggatat
    23101 tttgtaggtt aggattggag gatgataagc gtccggtgct ggtaatggtt tgattaaaat
    23161 ctgtatgtac tcttgcggtt tttggttcgg ctaatttcgg taaagcatcc acataggtgg
    23221 attttaattt ggataaggtg cgatgttcaa ggatataatc aactaaggga tgatctcctt
    23281 gtagtttctc taaaacactt tgatcggtgg agtaacctgt tttagttttt ctggattttt
    23341 tgcgattgag tgccaatcga tcaaagagga tttcacttaa ctgtttagga gagctaagat
    23401 taaaagtttc tccgatagat tcataagctt ttttttctat gtctgctagg tctctttcta
    23461 gttgctggga aaagtttttt agatagttaa tattaatttt tataccataa ttttccatct
    23521 gccaaagcac catcactaag ggagattcta cctctgagaa taattgatat aaagggggaa
    23581 ttttagttaa ttctgcctgt aatttttctc gaagtaaata ggtagcgtaa gcatctaatc
    23641 cgcagtagtt agcagtggtg gtaatatcta aatcagcgat ggtttttccc gcaggaatac
    23701 cgagactttt ataactaagg gaagtgatac cagttaaata gcgatcacat aaatcttcga
    23761 gattatgagt tagttcggga tggaggacat aactagctac catagtatca aaaactagac
    23821 ctttaacggt aattccttga tgataaaaaa catcaatatc aaatttcgca ttctggaaga
    23881 ctttcggata attatcaccc gctaaaattg ggcgtaattt ttcgaggact atctctagat
    23941 ttaaattatc accgctctgg tggccgatag gaatataggc catagaattt aattcttttc
    24001 cccaacagca accgatgccg actaatttgg ctacacgagg atttaaatcc gtggtttcgg
    24061 tatcccaagc gacgggaaaa tgtggattag tgaaagttgt taatttggcc agtaaagcat
    24121 ctaacttagg aatagtatca ataatttccg gagcaattag aaccggtgaa gaaatagggg
    24181 gtaaatcaaa cagagacaat tgattagaat cagcatcgct agttaattct agggtaggag
    24241 tgccacctag atgctgttga atttgatcga gtttctggag aattttcttt aactctaatc
    24301 ttgccaataa aggttgaact aaacgccgat caaaacctcg gagacgaaat ttctcttcct
    24361 cgatactaac gggggcatca agggcaattt tggctaaata gcgagaactt tcggcggcgg
    24421 tttttccctc taataattta gtttttacca ttcccttgat cgattccaga ttggtatata
    24481 tatcgtctaa gttatcgtat ttttttaata aatctatcgc tgttttttcg ccaattccgc
    24541 gcaccccggg tatattatca gacttatcac cgcaaagagc tttaaaatct actacctgtt
    24601 ttggtgtcac ccctaacttt tccgtgacag cttgggaatt aaattctgta taaccctgtc
    24661 ccgatgtact tttgaccgca ttaatatcca gatagagaac cgtggttttt ttctcctcat
    24721 ctaccaattg gaacaaatct cgatcgcctg ttaaaatctt gactgtataa ccattatcgc
    24781 cggcctgttt tgccaaagtt cctaaaacat catctgcttc ataacccgga gaagtcacaa
    24841 taggaatatt catcgctgcc aagagacttt gcagattagt taaatcgggg cgaaattctt
    24901 cgggagtttc ttgccgatta gccttataat tgacatcagc ttcatggcga aaagaaggag
    24961 ctgctaaatc aaaagccact gctaaataac tgggtttttg agtttctagt aactgcagca
    25021 gagaatttaa aaacccaaaa caaacactgg tgggaatgcc agtagaagta cgcagaggtc
    25081 ccgaacgaga cttagcaaag gcataatagg cacgaaaagc caaagaatgc ccatcgatca
    25141 ggataaatag cttattttgc tcggcactgg tcataatcta gcctctttag tcctatcaaa
    25201 ataatactaa atagtgtata aaatttacat cccctatttt gcccgaattt gcctaaattt
    25261 gacaagggag tttcttctgt attagactat aacagatgct ggccttgttc taaccttaaa
    25321 tctttctatc tctttacagg cggcggatgc ctgtgctaag ctaagacgca tttaaagtgc
    25381 ttattcttaa gattagccga atctccctca atccctttac tgttgcctct tgcctcttgc
    25441 ctcgtctcaa caagcaattt aaattacgaa cagcttacca attgcatcaa tgtcatttac
    25501 aggcatcctg tttgtaccac tagggaacta tcagaaaaat gcggattact gtaggatctg
    25561 ttttaaagct gttagcgaag ggaattatgc tacactagca tcaatatcta gtcaaaaagg
    25621 cttgtaattt atgactcctc atctttctct tagccagatt atagaatcta tcgatcaact
    25681 ttctttagaa gaacaaaacc aattatttga aaaattagcg cagaaaaaac aacaacaaga
    25741 gcatccactg ctcaatatac cagttacact aacctataat cccctcttag atgaagtcat
    25801 agagagtata gaaacctatc gtcaaaaagt tgaccaagaa gacagtcaac aagttgaaac
    25861 accgtgactc aatatatatt agataccgat catataaccc tactgcaaaa actacatccg
    25921 aacttagttc ggagagtagc gtctatcgat cctgatgata cggctataac aattattact
    25981 ttagaagaac aagtaagagg atggttcagc gtgatcagaa aatactctca aagcaatcaa
    26041 tatgaaaagt taatcaaagg ctatcaaggt ttgagagata tagttaatta tctgagcaaa
    26101 tttaaaattt tgaactatac tttagaagct cattatcatt tcagagaatt acgggagcaa
    26161 aaaattccta taggaacaca agatttaaga attgcagcga ttagtttatc agaaaacagt
    26221 attttagtaa cacgcaatta tcgtgatttc tctcaaatcc ctaatttacg cctacaagat
    26281 tggacgatat agaactctgt agggatgact acgaaaattg ttacattttt taaggattac
    26341 ccagccatta ttcagccatt agcctagcat cggcgatcgc tgataacacc ccgtcaaaaa
    26401 aaatatttgc caaaaaacca gcgtaagggt attttaagaa taattttata tgtttacttt
    26461 gcgattacat gagaatccta gttacgggtg gtgcaggttt tattggttcc catctaatcg
    26521 atcgcttgat ggaacagggt catgaagtgg tctgtctgga taacttctac acgggggcgc
    26581 gccgaaatat cgttaaatgg ctaggaaatc cttattttga gctaattcgt cacgatatca
    26641 ccgagccgat ccgtttagaa gtcgatcaga tttatcacct ggcctgtcct gcttccccaa
    26701 ttcactatca atataatccc gtcaaaacga ttaaaacgaa tgttttaggg accatgtata
    26761 tgctgggtct agctaaacgg gtaaaagcgc gatttttgct cgcttctacc tcggaagtct
    26821 atggcgatcc cgatgtgcat ccccaaacgg aagaatatcg aggtaatgtc aactgcattg
    26881 gaccgcgttc ctgttatg
//
