LOCUS       NZ_AP024565            21908 bp    DNA     circular CON 26-SEP-2024
DEFINITION  Microcystis aeruginosa strain NIES-88 chromosome, complete genome.
ACCESSION   NZ_AP024565
VERSION     NZ_AP024565.1
KEYWORDS    RefSeq.
SOURCE      Microcystis aeruginosa
  ORGANISM  Microcystis aeruginosa
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Chroococcales; Microcystaceae; Microcystis.
REFERENCE   1
  AUTHORS   Phan,C.-S., Matsuda,K., Balloo,N., Wakimoto,T. and Okino,T.
  TITLE     Argicyclamide A-C unveil enzymatic basis for guanidine
            bis-prenylation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5478552)
  AUTHORS   Matsuda,K. and Wakimoto,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2021) Contact:Kenichi Matsuda Hokkaido University,
            Graduate school of phrmaceutical sciences; Kita 12, Nishi 6,
            Kita-ku, Sapporo, Hokkaido 060-0812, Japan URL
            :https://www.pharm.hokudai.ac.jp/tennen/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 87x
            Sequencing Technology             :: DNBSEQ; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019704275.1-RS_2024_09_26
            Annotation Date                   :: 09/26/2024 11:16:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,602
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,071
            CDSs (with protein)               :: 5,071
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 479
            CDSs (without protein)            :: 479
            Pseudo Genes (ambiguous residues) :: 0 of 479
            Pseudo Genes (frameshifted)       :: 110 of 479
            Pseudo Genes (incomplete)         :: 380 of 479
            Pseudo Genes (internal stop)      :: 101 of 479
            Pseudo Genes (multiple problems)  :: 91 of 479
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 14:54:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 549161
            Orig. end                         :: 571069
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024565.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated at DFAST https://dfast.nig.ac.jp/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21908
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="2"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21908
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21908
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11908](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11908
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    complement(111..125)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 21.83"
                     /tool="antismash"
     gene            complement(123..1361)
                     /locus_tag="LC603172_RS02845"
                     /old_locus_tag="MAN88_05550"
     CDS             complement(123..1361)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002741658.1"
                     /locus_tag="LC603172_RS02845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05550"
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="WP_036395903.1"
                     /transl_table=11
                     /translation="MFDSPVDLLILSNGPGEISTWVRPVVKRLRQNLTEDRSLLRISVI
                     LSPCTHSMGAETEVVRKYPEVDRVQSPENFFNFLFWGKTADNWDWHPKGIVLFLGGDQF
                     FALTIGKRLGYRTVIYAEWEARWYRGIDRFAVMNTSVLNNIPQQYQHKFTVIGDLMVDL
                     PNAITPDDATLIALLPGSKPSKLAQGVPLTLAIAEKIHAQDPRTKFLIPVAPTLNLAYL
                     AKFADPSSNPMITKTGWSAAKLKNGEKPYLETDKGVKVDLITDFPAHNQLSRCHLALTT
                     VGANTAELGALAIPMIILLPTQQLDAMRTWDGLPGLLAQLPGVGSLFAKIINLYMLRKK
                     RLYAWPNIWAGAEIVPELLGELQPEEVANMASSWLENPQQLEAIRQKLRAVRGQPGAVD
                     KLVSIIAEQLSSF"
     gene            complement(1508..2668)
                     /locus_tag="LC603172_RS02850"
                     /old_locus_tag="MAN88_05560"
     CDS             complement(1508..2668)
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064: glucose-1-phosphate
                     adenylyl/thymidylyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012267092.1"
                     /locus_tag="LC603172_RS02850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05560"
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="WP_061432229.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 6.2e-09, bitscore:
                     34.7, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAMILAAGKGTRVRPITHTIPKPLIPILQKPVMEFLLELLRQHG
                     FDQIMVNVSHLAEEIESYFRDGQRFGVHIGYSFEGRIEEGELIGDALGSAGGLRRIQDF
                     NPFFDDTFVVLCGDALIDLDLTAAVKWHREKGAIATIVTKTVPREEVSSYGVVVSDEEG
                     RILSFQEKPAIDEALSTCINTGIYIFEPEIIDFIPPNSKYDIGGELFPQLVAKAAPFYA
                     LNMDFEWVDIGKVPDYWQAIRGVLSRKIKNVAIPGIEVKPGIYTGLNVGVNWDRVDITG
                     PVYIGAMTRIEDGAKIVGPSMIGPNCWICGGATVDNSVIFEYSRLGSGARLVDKLVFGR
                     YCVDKTGAAIDVEAAALDWLITDTRKVPPEFDHPKHQAIRSLFNGK"
     misc_feature    complement(3099..3113)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 20.41"
                     /tool="antismash"
     gene            complement(3375..>3512)
                     /locus_tag="LC603172_RS28750"
                     /pseudo=""
     CDS             complement(3375..>3512)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007304384.1"
                     /locus_tag="LC603172_RS28750"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="RNA-guided endonuclease TnpB family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="YIEDRDKMAALNILKKATIGHIGSWSLDLNAWGDLSSILVGRNTC
                     "
     gene            complement(3520..4506)
                     /locus_tag="LC603172_RS02855"
                     /old_locus_tag="MAN88_05570"
     CDS             complement(3520..4506)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012266743.1"
                     /locus_tag="LC603172_RS02855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05570"
                     /product="IS30-like element ISMae39 family transposase"
                     /protein_id="WP_221275333.1"
                     /transl_table=11
                     /translation="MSHQHLNIEQRNLLYQLSQEGNLSQRQMAVWLGCHQSTISRELKR
                     NQSSLGCYLPDTAQAQSETRRKNAKQPFKNVSESALELVKEGLKDYHSPEQIAGRLKRA
                     SQESLSHETIYQMIYQNYHGCGEYQKYLRRGLCQRKSRGGAKSKRGGIPGRVDISERPA
                     IADQKTEIGHWESDTMIGGNHLGVLVTHVDKASKFLVAGLAKNKTASEINRVTEKLFQE
                     IHPDKRKTFTCDNGKEFCGHQELSQKLGADFYFATPYHSWERGLNEHTNGLLRQFFPKG
                     TNFKIVKPEQVEIAVNLINNRPRKCLDYRTPNEVFYKGRSDSDAIQT"
     gene            complement(4536..5657)
                     /locus_tag="LC603172_RS02860"
                     /old_locus_tag="MAN88_05580"
     CDS             complement(4536..5657)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1192: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015224049.1"
                     /locus_tag="LC603172_RS02860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05580"
                     /product="transposase"
                     /protein_id="WP_260324507.1"
                     /transl_table=11
                     /translation="MFVLEYKLRGKPSQYQAIDEAIRTVQFVRNKCLRYWEDNKGVGQK
                     DVYKYVTQLRSEYPFVQDLNSTACQQACERTWTAILRFYNNCQNQIAGKKGYPKYSKRT
                     HSVEFKKSGWKLNRNSKRITFTDGKNLGELKLIGSRDLFDFQEWQIQRIRIVKRADGYY
                     CQLMLKLDARDITPQSESTGHCLGLDLGLKYLYADSNGNKVEPPKYYRKAEKRLNKLNR
                     RKSKKFKRGQKQSNNYKKASQKYAKGHLKVSRQREEFAKKLALRLIQSNDLIAYEDLKV
                     KNLVRNRKLAKSINDAGWSQLRKWIEYFGIKYGRLTIAVNPAYTSQECFNCGRLIQKSL
                     SVRTHVCLCGYRQFKNETHQPQELTKKGSSGTV"
     gene            complement(5809..6099)
                     /locus_tag="LC603172_RS02865"
                     /old_locus_tag="MAN88_05590"
     CDS             complement(5809..6099)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002732452.1"
                     /locus_tag="LC603172_RS02865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05590"
                     /product="DUF4090 family protein"
                     /protein_id="WP_061432231.1"
                     /transl_table=11
                     /translation="MSTETNLTTTTGSDAIDVAIANGIDFDGSPIPPAKLELYHRVMGL
                     EAGRQRSGVSNTMRSRIVRIGAKHIPQEELNQLLLAADFAPLKEKEIAFYL"
     gene            6120..6608
                     /locus_tag="LC603172_RS02870"
                     /old_locus_tag="MAN88_05600"
     CDS             6120..6608
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002783352.1"
                     /locus_tag="LC603172_RS02870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05600"
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="WP_002777269.1"
                     /transl_table=11
                     /translation="MLFYQAIDIRRYDQESNLHFCVTMAQLPTYQPKQLSLGPLEAEIL
                     NIVWELEPVSVKDVHDRILADPERDLAYTSVTTVLRRLTNKGWLSCYKQGRIFYWKPMV
                     SKEQAQAIKAYEQLHRFLAIGNADVVASFADSLDTASVEQLKAIASRLDTLRQQRRER"
     gene            6608..7456
                     /locus_tag="LC603172_RS02875"
                     /old_locus_tag="MAN88_05610"
     CDS             6608..7456
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731557.1"
                     /locus_tag="LC603172_RS02875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05610"
                     /product="M56 family metallopeptidase"
                     /protein_id="WP_061432232.1"
                     /transl_table=11
                     /translation="MHSLMLLLALTIAIGLRWFLPSYQRRWQITLFFFLFPPLLLLMTV
                     ISVVCMGYRGQMLGYNSSLISYFSAIIWLVFAIFCLIKLSYQTWQTHRDFSSYPLKKIT
                     AQKARVLAVDFPYSARVGFWKSELIVTQGLLNLLDQEHLQAVLAHEQAHQEYHDTFWFF
                     WLGWLRSMSSWLPNSENLWSELVFLRELRADKYASGQVDYLLLAESLLLVAEKVNQVAE
                     ISFSDSCCVALSDNSLNNRLLERIEALVESEKPEFPRFNYQVWLLLSLSLAPFLLLPLH
                     S"
     gene            7459..7926
                     /gene="bcp"
                     /locus_tag="LC603172_RS02880"
                     /old_locus_tag="MAN88_05620"
     CDS             7459..7926
                     /EC_number="1.11.1.24"
                     /GO_function="GO:0016209 - antioxidant activity [Evidence
                     IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="bcp"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002753380.1"
                     /locus_tag="LC603172_RS02880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05620"
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="WP_061432233.1"
                     /transl_table=11
                     /translation="MTLEVGQKAPEFATPNQRGEISKLADFAGQWLVLYFYPKDNTPGC
                     STEAIDFTALSPQFQQLNAVILGVSPDSEKSHCRFIEKHNLTIQLLSDPDHQLAEIYQV
                     WGLKKFMGKEYMGIKRSTFLIDHRGNIAYIWSNVKVKAHAEAVLKKLEELQ"
     gene            8083..8880
                     /gene="rpsB"
                     /locus_tag="LC603172_RS02885"
                     /old_locus_tag="MAN88_05630"
     CDS             8083..8880
                     /GO_component="GO:0015935 - small ribosomal subunit
                     [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpsB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002767849.1"
                     /locus_tag="LC603172_RS02885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05630"
                     /product="30S ribosomal protein S2"
                     /protein_id="WP_004162676.1"
                     /transl_table=11
                     /translation="MPVVSLAELLESGVHFGHQTRRWNPKMSQYIYTARNGVHIIDLVQ
                     TAQLIEEAYEFVRGEADRGKRFLFIGTKRQAAAIIKQEALRSGSHFVNQRWLGGMLTNW
                     ETIRGRVERLKELEELENSGALDKRPKKEASVLRRELGKLEKYLGGIKTMRRLPDLVVV
                     VDQRREYNAIQECQKLGIPIISLLDTNCDPDLVDVPIPANDDAIRSVKLILGKISDAII
                     EGRRGGQAAVEEYEEDYDNETEYEEEGDYSQYAAEFASGDDDN"
     misc_feature    8121..8135
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.37"
                     /tool="antismash"
     gene            8997..9752
                     /gene="tsf"
                     /locus_tag="LC603172_RS02890"
                     /old_locus_tag="MAN88_05640"
     CDS             8997..9752
                     /GO_function="GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tsf"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002753383.1"
                     /locus_tag="LC603172_RS02890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05640"
                     /product="translation elongation factor Ts"
                     /protein_id="WP_002734629.1"
                     /transl_table=11
                     /translation="MAEITAQQVKELREKTGAGMMDCKRALTENAGDITKAIEWLRQKG
                     ITSAEKKASRVAAEGMIGSYIHTGSRIGVLVEVNCETDFVARREEFKKLVNDVAMQIAA
                     CPNVEYVKVADIPAEISAKEKEIEMGRDDLANKPDNIKEKIVAGRIEKRLKELSLLDQP
                     FIRDQNISIDELLKQAIAALGENIQVRRFQRFVLGEGIEKEETDFAAEVAAQMGQKAPE
                     PVAAAPQVEEKAPEPAAKDNPPAKGKKKK"
     gene            10001..11908
                     /gene="shc"
                     /locus_tag="LC603172_RS02895"
                     /old_locus_tag="MAN88_05650"
     CDS             10001..11908
                     /EC_number="5.4.99.17"
                     /GO_function="GO:0016866 - intramolecular transferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="shc"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T2TS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002767853.1"
                     /locus_tag="LC603172_RS02895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05650"
                     /product="squalene--hopene cyclase"
                     /protein_id="WP_061432234.1"
                     /sec_met_domain="T2TS (E-value: 8.1e-132, bitscore: 438.8,
                     seeds: 58, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQIQDKITEIAAKTAKAIELSQNYLLSTQYSEGYWWAELESNVTI
                     TSEAILLHKIWKTDKKRPLDKAATYLRQQQCPNGAWELFYGDGGDLSTTVEAYMGLRLL
                     GIPANDPALEKAREFILAKGGISKTRIFTKMHLALIGCYDWQGVPSIPAWIMLLPENFP
                     FTIYEMSSWARGSTVPLLIVFDKKPVYKMGFNLDELYTEGVNNVKYELPKNNDWADVFL
                     WLDGLFKWAEKTDLVPFRQESLKAAEKWVIERQEDTGDWGGIIPAMLNSLLALKALGYD
                     VYDPIVARGLKAVDNFAIETDNTYCVQPCVSPVWDTAWVIRSLIESGLNPAHPAMIKAG
                     QWLIDQQILDYGDWAIKNKIGTPGGWAFEFDNRWYPDLDDSAVVVMALELIKMPDENIK
                     KAVMKRAVNWMATMQCKAGGWGAFDIDNDQNWLNSLPYADLKAMIDPNTADVTARVLEM
                     LGTCDVNMEENRVKKALDYLEKEQEADGSWFGRWGVNYIYGTSGALSALAFLEPNKYRH
                     QLQKGANWLSSCQNVDGGWGETCFSYNNPKFKGQGNSTASQTAWALIGLLAVGKVTGNY
                     QREVIEKGVNYLLVTQKENGTWDEDYFTGTGFPCHFYLKYHFYQQYFPLLALGRYRALI
                     "
     gene            complement(11969..12364)
                     /locus_tag="LC603172_RS02900"
                     /old_locus_tag="MAN88_05660"
     CDS             complement(11969..12364)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733792.1"
                     /locus_tag="LC603172_RS02900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05660"
                     /product="type II toxin-antitoxin system HigA family
                     antitoxin"
                     /protein_id="WP_004162674.1"
                     /transl_table=11
                     /translation="MFATDKYLELLKQYPPRPIHNEEDLEMMQEVINRLLDKPQLTAEE
                     REYLNVLGALIYEYEENQEPIPDIYGLELLKFILEEKNLQKQDLLSIFESKSTLDDILD
                     GQQELTPIYIQKLANFLNISPDLFFPS"
     gene            12483..13478
                     /locus_tag="LC603172_RS02905"
                     /old_locus_tag="MAN88_05670"
     CDS             12483..13478
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002752420.1"
                     /locus_tag="LC603172_RS02905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05670"
                     /product="glycosyltransferase"
                     /protein_id="WP_061432235.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 7.6e-08,
                     bitscore: 30.5, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRNLYFLLPGTTSPFGGGGLWAEMKTVNLASQICSAQIVTYRQKE
                     LNYPFLEDILAKISSDDSIFVVSWGFDVPKLVQRLRSHNIIYHAHSTGYGFRLPSQIPI
                     ITVSRNTMGYWGEKAPNALIYYLPNQIGSEFLNLGLERDIDVLVQGRKSSKYLLNRLVP
                     ALRPHCRVKVLDSYVEDLVGLFNRSRVYLYDSAEYWAVNGLTEGFGLPPLEALACGCTV
                     FSSVNSALADYLDPGFNCQKIGAYSSQYDLERILNYVRNFPVNSLPANFIDDYNPEKLL
                     PRFETILREINDFFDIKKRYPADIEGLEPLRLKKLWIQSSLAKLKKKLFK"
     gene            13707..14612
                     /locus_tag="LC603172_RS02910"
                     /old_locus_tag="MAN88_05680"
     CDS             13707..14612
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200659.1"
                     /locus_tag="LC603172_RS02910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05680"
                     /product="Npun_R2821/Npun_R2822 family protein"
                     /protein_id="WP_061432236.1"
                     /transl_table=11
                     /translation="MKRGIYITANDRVIEQAIALMNSIRLYDPDSPVILIPYDNNYQKI
                     ADLLSEKYGVILYPDLQLVEELAQKIYDIFGEKFFARPNQFRKQVCWFGELDQFLYIDT
                     DIVVFEKIIDNLNFLDTYDFLCCDYQHKKGITDVFNPIVLEQGIFTENDLSGMFNGGFW
                     ASKKNLFSEQELYSAFQECAAHPEYFDFSQKTSDQPIINYTILKRVPNRFNIVRAPGCQ
                     AGNWGGSSHFQPQGNILIDPRLNQPLKYLHWAGIRIEPGCPYWDIWKYYRYLDDPNPPA
                     DPPASKPKNPFQRLLDKIKL"
     gene            complement(14655..14945)
                     /locus_tag="LC603172_RS02915"
                     /old_locus_tag="MAN88_05690"
     CDS             complement(14655..14945)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004163895.1"
                     /locus_tag="LC603172_RS02915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05690"
                     /product="DUF3288 family protein"
                     /protein_id="WP_002767861.1"
                     /transl_table=11
                     /translation="MASKNQEQQHPQERLDRPIVDQLLQSEPNDLNLAECARLRIRYQN
                     FPGAREIQRDLDLILEKWQLDEASLWAKTRQLHSHGQVYQIRQTEEQQDWS"
     gene            complement(14948..15562)
                     /locus_tag="LC603172_RS02920"
                     /old_locus_tag="MAN88_05700"
     CDS             complement(14948..15562)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200656.1"
                     /locus_tag="LC603172_RS02920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05700"
                     /product="hypothetical protein"
                     /protein_id="WP_061432237.1"
                     /transl_table=11
                     /translation="MTKGNLNDLIRTEANKEVESQPSPSAKRPKETNATLQAKIKDLTA
                     ELEKTEKNGQTLQEKVISLEEELKEHLELSVSLQQLQQQTEQLESVLSEKTTLVAKLSS
                     QLSQSQTELTEKKQLIEKLSSQLSQSQTELTEKKQLIEKLYNQIKTLENSPTATPEPSA
                     ITKLLPKKPSLYNFEMATLARYIAPNPTPTELTDSDIGWFD"
     gene            15865..16077
                     /locus_tag="LC603172_RS02925"
                     /old_locus_tag="MAN88_05710"
     CDS             15865..16077
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002760983.1"
                     /locus_tag="LC603172_RS02925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05710"
                     /product="hypothetical protein"
                     /protein_id="WP_041804230.1"
                     /transl_table=11
                     /translation="MNIEEKARLNLTQERQAEGMLEENVLTRTEELLGEGEAGTAKARV
                     LLTENRQEDEKLQDKILERSIEAMQ"
     gene            16217..17107
                     /gene="trpC"
                     /locus_tag="LC603172_RS02930"
                     /old_locus_tag="MAN88_05720"
     CDS             16217..17107
                     /EC_number="4.1.1.48"
                     /GO_function="GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002752425.1"
                     /locus_tag="LC603172_RS02930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05720"
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="WP_061432238.1"
                     /transl_table=11
                     /translation="MQIRRKHPNPAVKVESLSYVVKVPEAQPQNILEEIVWHKEIEVDK
                     LRERLPLLELRQKIANTAPPCDFLAALKQGKTQPALIAEVKKASPSKGVILEDFDPVAI
                     ARTYEQGGATCLSVLTDSKFFQGSYENLSLVRQAVSLPLLCKEFILYPYQIYYARSKGA
                     DAVLLIAAILSDQDLAYFVKIVKGLGMTALVEVHSLAEFDRVLAIEGIELIGINNRNLE
                     TFTVDLDNTRQLLEARGEQVREKGILIVSESGLHTATDLAKVKQAGANAVLIGESLVKL
                     PDPALGIQKLFENRE"
     misc_feature    complement(17252..17268)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.7"
                     /tool="antismash"
     gene            17343..18497
                     /locus_tag="LC603172_RS02935"
                     /old_locus_tag="MAN88_05730"
     CDS             17343..18497
                     /NRPS_PKS="Domain: Aminotran_5 (5-343). E-value: 1.3e-64.
                     Score: 210.3. Matches aSDomain:
                     nrpspksdomains_LC603172_RS02935_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002760980.1"
                     /locus_tag="LC603172_RS02935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05730"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_061432239.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.5e-32, bitscore:
                     111.3, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.8e-05, bitscore:
                     22.4, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENKNMLMIPGPTPVPEAVLLAMAKAPIGHRTGAFSKVIAELTEN
                     LKWLHQTSGDVLMLTASGTGVMEAGIINFLSPGDRVLVGDNGKFGERWAKVAKAYGLNV
                     DVVKAEWGKPLDPEAFTAKLTEDKEKAIKAVIITHSETSTGVLNDLESINKAVKDHGEA
                     LIIVDAVTSLGAYHIPIDEWGLDVVGSGSQKGYMIPPGLGFVSVSQKAWKAYETAKLPR
                     FYLDLGKYKKATDEDSSPFTPPVNLMYGLQASLQMMRREGLAAIFARHQRLTKMTRAGM
                     QALNLPLYAADSCASTAITAVAPTLVDAEKIRSTLNKKFDIALAGGQDHLKGKIFRIGH
                     LGFASERDILTVIAAIEATLIELGVEGVTPGAGVAAAASVMVQG"
     aSDomain        17358..18371
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LC603172_RS02935_Aminotran_5.1"
                     /evalue="1.30E-64"
                     /label="LC603172_RS02935_Aminotran_5.1"
                     /locus_tag="LC603172_RS02935"
                     /protein_end="343"
                     /protein_start="5"
                     /score="210.3"
                     /tool="antismash"
                     /translation="MLMIPGPTPVPEAVLLAMAKAPIGHRTGAFSKVIAELTENLKWLH
                     QTSGDVLMLTASGTGVMEAGIINFLSPGDRVLVGDNGKFGERWAKVAKAYGLNVDVVKA
                     EWGKPLDPEAFTAKLTEDKEKAIKAVIITHSETSTGVLNDLESINKAVKDHGEALIIVD
                     AVTSLGAYHIPIDEWGLDVVGSGSQKGYMIPPGLGFVSVSQKAWKAYETAKLPRFYLDL
                     GKYKKATDEDSSPFTPPVNLMYGLQASLQMMRREGLAAIFARHQRLTKMTRAGMQALNL
                     PLYAADSCASTAITAVAPTLVDAEKIRSTLNKKFDIALAGGQDHLKGKIFRIGHLGF"
     gene            complement(18698..19510)
                     /locus_tag="LC603172_RS02940"
                     /old_locus_tag="MAN88_05740"
     CDS             complement(18698..19510)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002767868.1"
                     /locus_tag="LC603172_RS02940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05740"
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="WP_061432240.1"
                     /transl_table=11
                     /translation="MFDNTCKFLAENFSEDFASWLLGEPITMTQLSPSELSLEPIRADA
                     LILLNSDSDVLHIEFQTEPDPIMPFRMADYRLRGFRRFPQKRMHQVVIYLTRSTSPLVH
                     QTTFEIPGTRHEFEVIRLWEQPTQTFLEATGLLPLAVLTQTPDTAQTLRQIATRIEAIP
                     DQRIQSNVAASTGILAGLLLNKELINQVLRRDIMQQSVIYQEWREEFRQEGECSLILRL
                     LSRRIGEVTPEQRSQIQALSINQLEALGEALLDFTKPGDLEEWLRSHL"
     misc_feature    19462..19476
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.74"
                     /tool="antismash"
     gene            complement(19532..20392)
                     /locus_tag="LC603172_RS02945"
                     /old_locus_tag="MAN88_05750"
     CDS             complement(19532..20392)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002776177.1"
                     /locus_tag="LC603172_RS02945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05750"
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="WP_061432241.1"
                     /transl_table=11
                     /translation="MKRDSIFYRLFQQSPSLLFDLLENPPDNATEYRFDSVAVKEPKFE
                     IDGVFLPPDTDRNGVIYFCEVQFQKDERLYERVFGESFLYFYRQRERFTDWQIVVIYPT
                     RSLEQSNTHPYRSLLNCEQVHRVYLNELGEIQQLPLGVALMVLTTVEETQAPEKARYLL
                     ARTQEQIVDTEASRAIIEMIATIMVYKFTNLSRQEVDTMLGLQLADTRVYREAKEEGRQ
                     EGESALILRLLSRRIGEVTPEQRSQIQALSINQLEALGEALLDFTKPGDLEEWLRSHLE
                     AEWLR"
     gene            complement(20511..20918)
                     /locus_tag="LC603172_RS02950"
                     /old_locus_tag="MAN88_05760"
     CDS             complement(20511..20918)
                     /GO_function="GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /locus_tag="LC603172_RS02950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05760"
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="WP_061432242.1"
                     /transl_table=11
                     /translation="MRVVVDTNILVSFAIRPNENFEQMFNFLARQGITLISDDTVAELF
                     TVLNREKFRKYIRQDSAMDYIEWYVGISESVTVTESVIACRDPKDDKFLSLAVAGKADC
                     IIAGDSDLLDMIAYEGIPIYRATDFLKLFCE"
     gene            complement(20918..21127)
                     /locus_tag="LC603172_RS02955"
                     /old_locus_tag="MAN88_05770"
     CDS             complement(20918..21127)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002789010.1"
                     /locus_tag="LC603172_RS02955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MAN88_05770"
                     /product="hypothetical protein"
                     /protein_id="WP_061432243.1"
                     /transl_table=11
                     /translation="MTTQATVIQGTAKEIREALALIPDDEIVRLMVGRPSLSIIARKLQ
                     HEAATLGMTAAEHDALMASLKNDW"
ORIGIN
        1 aacaattaat taattctggc gacagcaatc aaagggaatt ttatcagcag gaattttatc
       61 aaactgttaa taaattaatc gaaattgatc ccctctacca acaagcttaa ttctctaatt
      121 gattagaaac tagacagttg ttcagcaata atagatacta acttatccac tgctcctggt
      181 tgcccgcgca ctgcccgcaa tttttgccgg atagcctcta attgttgggg attttctaac
      241 caagaactag ccatatttgc cacttcttct ggttgtaatt cccccaataa ttccggaaca
      301 atttcggcac cggcccaaat atttggccaa gcatataaac gctttttccg caacatatag
      361 agattaatta tcttagcaaa taaagaaccc acccccggca gttgggctaa taaaccgggt
      421 aaaccgtccc aagttctcat cgcgtctagt tgctgagtcg gtagtaaaat aatcatcggg
      481 attgccagcg cccctaattc ggcagtatta gcacccaccg tcgttaaagc taaatgacag
      541 cgagaaagct ggttatgggc gggaaaatcg gtaattaggt caacttttac ccccttatct
      601 gtttctagat agggtttttc gccgtttttt aatttggcag cactccaacc ggttttagtg
      661 atcatcggat tagagctagg atcggcaaat ttcgccaaat aagctaaatt tagggtagga
      721 gcgacgggaa ttaagaattt agtccgggga tcctgcgcgt gtatcttttc ggcaatggct
      781 agggttaggg gtacaccctg agctaatttc gagggtttgg aaccagggag aagtgcaatt
      841 aaggtagcat catcaggggt gatagcattg ggcaaatcca ccattaaatc gccgataacg
      901 gtaaatttat gctgatattg ttggggaata ttatttaata cacttgtatt catcacggca
      961 aagcgatcga tcccccgata ccaacgcgct tcccattccg catagataac ggtgcgataa
     1021 cccaaacgtt taccgatggt gagggcaaaa aattgatcgc cccctaaaaa taaaacgatg
     1081 cccttggggt gccaatccca attatcggcg gtttttcccc agaaaagaaa attaaagaag
     1141 ttttctggcg actggactcg atcgacctcc ggatatttcc taactacttc cgtttccgcc
     1201 cccatagaat gagtacaagg ggaaagaatc accgaaatcc gcaacagcga tcgatcttct
     1261 gttaagtttt gtcgcaatct cttgacaact ggacgcaccc atgtagatat ttctcctggg
     1321 ccattagaga gaatgagaag atcaacagga gaatcaaaca tgataagtag tcgtgcaaaa
     1381 ttaatgtctt ggttaggata ggcaaaaggc aaaaggcaaa aggcactcat gcaaggagtg
     1441 gttagatatg tgtaattaat tttgcttagg tacttatcaa gtaaaaagtt ccccgttgcg
     1501 gtgttgccta cttgccattg aataaagaac gaattgcctg atgtttaggg tgatcaaatt
     1561 ctgggggaac cttgcgggta tcggtaatca accaatccaa ggctgccgct tccacatcga
     1621 tcgccgcgcc ggttttatca acacagtagc gcccaaagac caacttatcc accaaacgcg
     1681 cccctgaacc gaggcgagaa tattcaaaaa taacgctatt atccactgtt gcgccgccac
     1741 aaatccaaca atttgggcca atcatactcg gaccgacaat tttcgccccg tcttcaatgc
     1801 gcgtcattgc cccgatatac acaggtccgg tgatatcgac tcgatcccag tttaccccca
     1861 catttaagcc cgtgtagatg cccggtttga cttcgatgcc ggggatagca acatttttaa
     1921 ttttcctgga taacaccccg cgaattgcct gccagtagtc ggggacttta ccgatatcca
     1981 cccattcaaa atccatgttt aaagcgtaga aaggcgcagc tttagcgact aattggggga
     2041 acaactcgcc cccgatgtcg tatttactat tagggggaat aaaatcaatg atttctggct
     2101 caaaaatata gatacccgtg ttaatgcagg tacttaaagc ttcatcgatg gccggttttt
     2161 cttggaaact gagaatccgt ccctcctcgt cgctaactac caccccataa ctggatactt
     2221 cctctctggg gacggttttg gtgacaatag tggcgatcgc tcccttttcc cgatgccatt
     2281 taacggctgc ggttaaatca agatcgatca gcgcatcacc acagagaacc acaaaagtat
     2341 cgtcaaaaaa gggattaaaa tcttgaattc gtcgtaaacc gcccgccgat cctagggcat
     2401 caccgattaa ctcgccctct tcgattctac cctcgaaaga atagccgata tgcaccccaa
     2461 aacgctgacc atcgcggaaa taactctcaa tttcttcggc taaatgacta acattgacca
     2521 tgatctgatc aaagccatgc tgtcgcaaca gttccaataa aaactccatc accggctttt
     2581 gtaaaatcgg gatcaggggt ttgggaatcg tatgggtaat cggacggaca cgagttcctt
     2641 tgcctgccgc caaaatcatg gctttcatcg atgtttattt ccttttgcga tcgaggtttg
     2701 taattaaatc atgcctagtg tatcattact ggctcggaat cagtggttat cgatcctctt
     2761 gggggtacct cgaaaaatag atttacttca gcattgcacc gcctgaaacc ctgatcaaac
     2821 cttggtttac tctcaattag tgcgaagatt ttctcaataa gtccaattga tcgagctacc
     2881 ccttgagtaa ttgataacgg gaaaaagtga gagatttaac ccagattaca ctgatggaca
     2941 aaattaacaa atcaactttg ctgtaactca tactactcag tcctctaggc taagatgatc
     3001 aaaaaaacgc aagtttttaa attgatgggc aaaaaaagtt taattttagc caataaccaa
     3061 ggcgttatgt ctaggctacc tcgaaaaatc gaattttgag ggacggagcg ccttgttaat
     3121 tatttaaaat tactccctct tcatcaatgt agatcaagga gatttcctcg accaacactt
     3181 tgttaacaaa atcttttatt cgaggtgtcc ttgagcatta gatcaaccta gactccttgc
     3241 ctaatttccc caacaatccg gttaacccca agaagtctag gcgaaaaatg gtgagcaaaa
     3301 atttgactac ttgagatatt gacactcccg tcgcgcttat gcgagggatt cttggttcac
     3361 tgactcaagt tgtcttagca ggtatttcta ccaaccaaaa tagagcttaa atctccccaa
     3421 gcgtttaggt ctaacgacca gcttccgata tgccctatcg tagctttttt gagtatattt
     3481 aatgccgcca tcttgtccct atcttctata taggccaatt caagtctgaa ttgcatcact
     3541 gtctgatcta cctttataga atacctcatt cggggtacga tagtcaagac attttcgagg
     3601 acgattgtta atcaagttta cggctatttc cacctgctct ggcttaacaa ttttaaaatt
     3661 cgttcccttg ggaaaaaatt gtcttaatag tccattagta tgctcgttta atcccctctc
     3721 ccaagaatga taaggagtag caaaataaaa atctgctccc aatttctgac tcaactcttg
     3781 atgtccacaa aattctttgc cgttgtcaca agtaaaggtt tttcttttgt cagggtgaat
     3841 ctcttgaaaa agtttttctg tgactctatt gatttccgat gcggttttgt ttttggctaa
     3901 tcctgctact agaaatttcg aggccttgtc tacatgggta acgagaactc ctagatgatt
     3961 tcctccaatc atcgtgtcac tttcccaatg accgatctcg gttttctggt cagcaattgc
     4021 tggtcgctcg ctgatatcta cacgccctgg aatccctcca cgcttacttt ttgctccgcc
     4081 tcgactcttt ctttgacaaa gcccccggcg taaatatttc tggtactctc cacatccgtg
     4141 atagttctga tagatcattt gatagatggt ttcgtgactg agagattctt gactggctct
     4201 tttcagacga cctgctattt gttcgggact atgataatct ttcaatcctt ctttgaccaa
     4261 ctctaaggct gactcgctga catttttaaa gggttgtttg gcattttttc gccttgtctc
     4321 gctttgagct tgtgctgtgt caggtagata gcagccaagg gaactttgat tcctttttaa
     4381 ttcccgtgat attgtgcttt gatgacatcc gagccagacg gccatttgcc gttgagataa
     4441 atttccctcc tgactaagtt ggtagagcaa gtttctttgt tctatgttaa gatgttggtg
     4501 gctcattttt gggtctgatt tgtttttgtt aatgatcaga cagtacctga tgagcctttc
     4561 tttgtcaact cttgaggttg atgcgtttca tttttgaatt ggcgataccc acacaaacaa
     4621 acatgagttc taactgatag agacttttgg attaaccgac cacaattaaa gcattcttgg
     4681 ctagtatagg ctggattaac tgcaatagtt agcctgccgt atttaatgcc aaaatactca
     4741 atccattttc tcaattgtga ccaaccagca tcattgatac tcttggctag ttttcgatta
     4801 cgcacaaggt tcttgacttt taaatcttca tatgctatca agtcgtttga ctgaattaaa
     4861 cggagtgcca attttttagc aaactcttct cgttgcctac ttacttttaa atgtcccttg
     4921 gcgtattttt gactagcttt tttgtagtta ttagactgct tttgtcctct cttaaacttt
     4981 ttcgactttc ttctattcag tttattaaga cgcttttcgg cttttcgata atacttagga
     5041 ggctctactt tattcccgtt actgtcagca taaagatatt ttaaccccag atctaatcct
     5101 aggcaatgac ccgtcgattc tgattgtggg gttatatctc tagcatcgag ttttagcatt
     5161 aactggcaat aatatccatc agccctttta actattctta tccgttgaat ttgccattct
     5221 tgaaaatcaa acaagtctcg actaccaatt agttttaact ccccaaggtt ttttccatca
     5281 gtgaaagtta ttctcttgga atttcgatta agtttccaac ctgacttttt aaactcaaca
     5341 gaatgagtac gtttagagta tttaggatat ccttttttac cagcgatttg gttctggcaa
     5401 ttgttataga acctaagaat agcagtccaa gtccgttcgc aagcctgttg acaagctgta
     5461 gagttaaggt cttgaacaaa agggtattca cttcttaatt gagtgacata tttatataca
     5521 tccttttgtc ctacaccttt attgtcttcc caatatctaa gacatttatt ccgaacaaac
     5581 tgtactgtcc gaatagcctc atcaatggct tgatattgag atggttttcc tctaagcttg
     5641 tactctaaaa caaacatttt gcgttatcga cctaccacgc aaactactct agcatactaa
     5701 aattaaattg gcaactatag attagagtta ttcttagtcg tgcttcgctg ttctatgctg
     5761 gcggaacttt tcatctccga ggtgaaaccg agagatgaaa agccgacatt ataggtaaaa
     5821 agcgatttct ttctccttga gaggggcaaa atcggcggct aacaacagct gatttaactc
     5881 ctcttggggg atatgtttgg ccccgatgcg gacaattcgc gatctcatcg tgttagatac
     5941 tccgcttctt tgtcgtcctg cttccaaacc cattacccgg tgataaagtt ccaattttgc
     6001 cgggggaatt ggggaaccat caaaatcaat gccattagcg atcgccacat caatggcatc
     6061 agagcccgta gttgtggtta aattagtttc ggtggacata aagacaatat aaaggatcgt
     6121 tgctatttta tcaggcgatc gatatccgcc gctatgatca ggaaagtaat cttcatttct
     6181 gtgtgactat ggctcaattg ccgacctatc aaccaaaaca gttatccctt ggccccctag
     6241 aagcggaaat tctcaatatt gtctgggaat tggaacccgt tagcgttaag gatgtccacg
     6301 atcgcatttt agcggatcca gaacgggatc tagcctatac ttctgtgact acggtactgc
     6361 gtcgtttaac taataaaggc tggttaagtt gttataaaca gggtcgaatt ttctactgga
     6421 aaccgatggt ttctaaggaa caagcacagg caattaaagc ctacgaacaa ttacatcgtt
     6481 ttttggctat tggtaacgcg gatgtggtgg cttcttttgc cgatagtctc gatactgcta
     6541 gtgtcgaaca attaaaagcg atcgcctctc gtttagatac cctgcgtcaa caacggagag
     6601 aacgctaatg cacagtttaa tgttattact agcattaact atcgccattg gtttacgttg
     6661 gtttctgcct agctatcagc gtcgttggca gataacgcta tttttcttcc tctttcctcc
     6721 tttgctgttg ttaatgacag ttatatcggt ggtttgtatg ggctatcgcg ggcaaatgct
     6781 aggctataac tccagtttaa ttagttactt tagtgctata atttggctag tttttgctat
     6841 attttgtttg ataaaactat cctatcaaac ttggcaaacc catcgggatt ttagcagtta
     6901 tcccctcaaa aaaatcaccg ctcagaaagc gagagtttta gctgtggatt ttccctacag
     6961 tgccagagtt ggcttttgga agtcggagtt aattgtaacc caaggattat taaatctctt
     7021 agatcaagaa catttacaag cagttttagc ccacgaacaa gctcaccaag aatatcatga
     7081 cactttctgg tttttctggt taggttggtt gcgatcgatg tcttcttggc tgcccaatag
     7141 cgagaattta tggtcagaat tggtcttctt gcgggaatta cgcgccgata agtacgcttc
     7201 cggacaagtg gattatttac tattagctga atcgctgctt ttagtggcag aaaaagttaa
     7261 tcaagtagcg gaaataagtt tctctgatag ttgctgtgtc gctctgagtg ataattcctt
     7321 aaataatcgt ttattagaga gaattgaagc gttagtcgag tcagaaaaac ccgaatttcc
     7381 cagatttaac tatcaagtct ggctattgct ttccctctcc ctcgctcctt tcttactctt
     7441 gcctttacac tcctaaaaat gaccctagaa gttggacaaa aagcccccga atttgccacc
     7501 cccaaccaaa gaggcgaaat tagtaaatta gcagattttg ccggtcaatg gttggtctta
     7561 tatttttatc ccaaagataa cactccgggc tgcagcacag aagcgatcga ttttaccgct
     7621 ttgtcgccgc aatttcagca attaaatgcg gtaattctgg gagttagtcc cgattcagaa
     7681 aagtcccact gtcgttttat tgaaaaacac aatttaacta ttcaattatt gagcgatccc
     7741 gatcatcaac tggcggaaat ttatcaagtc tggggattaa aaaaatttat gggtaaggaa
     7801 tatatgggga ttaaacgatc cactttcctg atcgaccacc ggggaaatat tgcctatatt
     7861 tggtcaaatg tcaaggtaaa agcccatgca gaagcggttt tgaaaaaact tgaggaattg
     7921 cagtaggaag ccctcagtac ccaatattta cgggtatgca attgcgccct ttcgtgttag
     7981 actagaaaag ttgtcaaaaa tcgcacatct aggcgatcgg gtgtttctag gaatctagaa
     8041 atccagccct agatggagga taaacccgaa aaggagaaaa atatgcccgt tgtctctctt
     8101 gcagaattgc tagagtctgg ggttcacttc ggccatcaaa cgcgccgttg gaacccgaaa
     8161 atgtctcagt acatctacac tgcccggaat ggggttcata tcattgattt ggtgcaaact
     8221 gcccaattaa tcgaagaagc ttacgaattt gtccgaggag aagccgatcg cggtaaacgc
     8281 tttttattca tcggtacgaa acggcaagcg gcagcaatca ttaaacagga agctttacgc
     8341 agcggtagcc acttcgttaa ccaacgctgg ttagggggaa tgttaaccaa ctgggaaacc
     8401 attcgcggcc gggtagaaag actcaaagaa ttagaagaat tagaaaatag cggcgccctc
     8461 gataaacgac cgaaaaaaga agcatcggta ctgcgtcggg aattaggcaa actagaaaaa
     8521 tacctcggtg gcattaaaac catgcgccgg ctgccggatt tagtcgtcgt agtggatcag
     8581 cgtcgggaat acaacgctat ccaagaatgc caaaaattgg gcattcccat catctccctc
     8641 ttggatacta actgcgatcc tgacttggta gatgtgccga ttcccgccaa cgatgacgca
     8701 attcgctcag tcaaactgat tttaggcaaa attagcgatg cgatcatcga aggtcgtcgc
     8761 ggtggtcaag cagcggtgga agaatacgag gaagactacg ataatgaaac cgagtacgag
     8821 gaagaaggtg attattccca atacgccgcc gaatttgcca gtggagacga cgataactag
     8881 ggaaaattag ctattgatcg ccgctaggtt cggggggaaa ctttccgagc ctatcgagcg
     8941 gtcaaacctg cccctctcgt ggcatgatca attataagca gtaggttagg aattttatgg
     9001 cagaaattac agcacaacag gttaaagaac ttagggaaaa gaccggcgcc ggcatgatgg
     9061 attgcaagag ggcgctgacg gaaaacgcgg gggatataac caaagcgatc gaatggttgc
     9121 gtcaaaaagg gattacttcc gccgagaaaa aagccagtcg ggtagcggca gaagggatga
     9181 tcggcagtta catccacacc ggcagccgca tcggtgtttt agtagaagtg aactgtgaaa
     9241 ccgattttgt tgcccgtcgc gaggagttca aaaaattggt taatgacgtg gcgatgcaga
     9301 ttgccgcttg tcctaacgtc gaatatgtga aagtggccga tattcccgcc gaaatctctg
     9361 ccaaagaaaa agaaattgag atgggacggg acgatttagc caataaaccc gataatatca
     9421 aagaaaaaat cgtggctggc cgtattgaaa aacgtttgaa agaactttcc ttgctcgatc
     9481 aaccctttat ccgcgatcag aacatcagta ttgacgaatt gcttaaacaa gcgatcgccg
     9541 ctttgggaga aaatattcag gtgcgtcgtt tccaacgctt tgtactaggt gaagggatcg
     9601 aaaaagaaga aacggatttc gccgccgaag tagccgccca aatgggacaa aaagccccgg
     9661 aacccgtagc agccgccccg caagtggagg aaaaagcccc ggaaccggca gcgaaggata
     9721 atccgcccgc taaaggcaaa aagaaaaagt aagttcaatg aagatcaatc tggtggggtg
     9781 cgagtttcgt accctttttg tcttcaggga attatgaatt atgaatatgg acgggtcgga
     9841 aagcgtaaga ctactgctaa ggtagcagca tcctgtgaag cgtcaacccc attgacgcga
     9901 tcattctaaa aaggtggcgt ggtgaggaat caccgtccgt ttacggcggt gaggatgtca
     9961 attgcactcg tggtctctag aggttgtcga acggaaaaaa atgcagattc aggacaaaat
    10021 aaccgaaata gccgctaaaa cagcgaaagc gatcgaactt tctcaaaatt atttactttc
    10081 tacccaatac tctgagggtt actggtgggc agaattagaa tctaatgtca ccatcacctc
    10141 agaagcaatt ttactgcaca aaatctggaa aactgacaaa aaacgccccc tagacaaagc
    10201 cgccacctat ctgcgtcagc aacagtgtcc taacggcgct tgggagttat tttatgggga
    10261 tggtggcgat cttagcacca ctgtggaagc ctacatgggg ctgcgactat tgggaattcc
    10321 cgctaatgat cctgctttag aaaaagctag agaatttatc ttagccaaag gtggcatcag
    10381 caaaacccgc attttcacca aaatgcacct cgctttgatt ggttgttacg attggcaggg
    10441 ggttccctct atccccgctt ggattatgct actgccggaa aatttccctt ttactatcta
    10501 tgaaatgtcc agttgggcgc ggggaagtac ggttccttta ttaattgttt tcgacaaaaa
    10561 acccgtgtat aaaatgggtt ttaatctcga tgaactctat acagaagggg tgaataatgt
    10621 caagtatgag ttaccaaaaa ataacgattg ggcggatgtt tttctctggt tagatgggct
    10681 gtttaaatgg gcagaaaaaa ctgatttagt tcccttccgt caagaaagtc tgaaagcggc
    10741 ggaaaaatgg gtaattgaac gacaggaaga cacaggcgat tgggggggaa ttatcccggc
    10801 gatgttaaat tccctattag ctttaaaagc cctaggttat gatgtttacg atccgattgt
    10861 tgcccggggt ttaaaagcgg tggataattt tgccattgag acagataata cctattgtgt
    10921 acagccctgt gtttcgccgg tttgggatac ggcttgggta attcgatcgc taattgaatc
    10981 tgggctaaat cccgctcatc cagctatgat taaagcggga caatggttaa tcgatcaaca
    11041 aattcttgat tatggcgatt gggcgattaa aaataaaatc ggaacccccg gcggttgggc
    11101 ttttgaattt gacaatcgct ggtatcccga tttagacgat tcggcagtgg tagtgatggc
    11161 tttagagtta attaaaatgc ccgatgaaaa tattaaaaaa gctgtgatga aacgggcagt
    11221 taattggatg gctactatgc agtgtaaagc tggtggttgg ggagcttttg acatcgataa
    11281 cgatcaaaac tggcttaatt ctttgcccta tgccgatcta aaagcaatga tcgatcctaa
    11341 tacggctgat gtgactgcca gagttctaga aatgttaggt acttgtgatg tcaacatgga
    11401 ggaaaatcga gttaaaaaag ctttggatta tctggaaaaa gaacaggaag ctgatggtag
    11461 ttggtttggt cgttggggtg taaattatat ttatggaacc agtggggcat tatctgcttt
    11521 ggcttttctg gaaccgaata aatatcgcca tcaattgcaa aaaggagcca attggttaag
    11581 cagttgtcaa aatgttgatg gtggttgggg tgaaacctgt tttagttaca ataaccctaa
    11641 atttaaagga cagggaaata gtaccgcttc ccaaactgct tgggcattaa tcggtttatt
    11701 agccgtcggg aaagtcaccg gcaattatca gcgagaagtg attgaaaagg gcgttaacta
    11761 tcttcttgtt acccaaaaag aaaatggtac atgggatgaa gattacttca ccggtacagg
    11821 ttttccctgt catttttacc tgaaatatca tttctatcag cagtatttcc ctctactggc
    11881 tttggggcgt tatcgggctt tgatttagtc cgttaaccta tgcgttaaga caggaaataa
    11941 gcttaactgt ctagcgcatt ttttgtgact aactaggaaa aaataaatca ggagatatat
    12001 ttaaaaaatt ggctaatttt tgaatataga taggagttaa ctcttgttgg ccatcaagaa
    12061 tgtcatccag ggttgacttg ctttcaaaaa tagatagcaa atcttgcttt tgtaggtttt
    12121 tttcctctaa tatgaatttc aacaattcaa gtccataaat atcaggtatt ggctcttgat
    12181 tttcctcata ctcataaatt aaagccccta aaacatttag atattccctt tcttctgccg
    12241 ttaattgggg tttgtctaaa aggcgattaa taacctcctg catcatttct aagtcttcct
    12301 cattgtgaat aggacgagga gggtattgct ttaataattc taagtattta tcagtggcaa
    12361 acataattta ataatatcta tggttgatcg actattgcct ttagatacct cacaccttaa
    12421 taattaagta tagaatagcc aagtctccaa actaccgctt aaccaaaact tctattatcc
    12481 tgatgagaaa tctctatttt cttttgcccg gtactacttc tccttttggt gggggtggac
    12541 tgtgggcaga aatgaaaact gtcaatttgg ctagtcaaat ttgtagcgcc cagattgtga
    12601 cttatcgtca aaaagagtta aattatcctt ttttagagga tatactcgcg aaaataagct
    12661 cggatgacag tatttttgtg gttagctggg gttttgatgt tcctaaattg gtgcaaagat
    12721 tgcgatcgca taatatcatt tatcatgccc atagtacagg ttacggcttc cgtttaccct
    12781 cccaaatacc aattatcact gttagccgta atactatggg gtattgggga gaaaaggctc
    12841 ctaatgcttt aatttactat cttcctaatc aaattggctc agaatttctc aatttaggct
    12901 tagaaagaga tatcgatgtc ctagtacagg ggcgcaaatc ctcaaagtat cttctcaatc
    12961 gtctagttcc cgccctccgt ccccactgcc gcgttaaggt tttagatagc tatgtggagg
    13021 atttagtggg attattcaat cgcagtcggg tttatctcta cgattcggct gaatattggg
    13081 ctgtaaacgg tttaacggag ggttttggct taccgcccct agaagcttta gcctgtggtt
    13141 gtacggtatt ttctagtgtt aatagcgccc tagccgatta tctcgatccc ggtttcaatt
    13201 gtcaaaaaat cggggcttat tccagtcaat acgatctgga acgtattctt aattatgtcc
    13261 gtaattttcc cgttaattct ttaccagcta attttatcga tgattataat cccgaaaaat
    13321 tactaccacg ttttgaaaca attttaagag aaattaatga tttctttgat attaaaaaac
    13381 gctatccggc tgatatagaa ggtttagaac ccctgcgact taaaaaatta tggatacaga
    13441 gttctctggc taaacttaag aaaaaactgt ttaagtagca gtgatatttc cttaattatt
    13501 cgctaattca aaaatgaaac ccacgacttc ttaagagttg acagagaaag accgatcggc
    13561 tattcagtga tcagtgatca gttatcagtg aacagggata tcgggagaaa aaagctgatg
    13621 gctgactccc caaaaggaaa actttctact tcaccattaa gataactgct atataattat
    13681 ttctaactct tacccccagg atatttatga agcgaggaat ttacattacc gctaatgatc
    13741 gagttatcga gcaggcgatc gcattaatga atagtattcg tttgtacgat ccagattctc
    13801 ccgtcatctt gattccctac gacaataatt atcaaaaaat tgccgattta ttgtccgaaa
    13861 aatatggcgt tattctctat cccgatctgc aattagtgga agaattagcc cagaaaatct
    13921 atgatatttt tggagaaaaa ttctttgctc gtcccaatca atttcgcaag caggtgtgtt
    13981 ggtttggaga attagatcaa tttctttata tcgatacgga tatcgtagtt tttgaaaaaa
    14041 taatagataa tctaaatttc ctcgatacct acgattttct ctgctgtgat taccaacaca
    14101 aaaaagggat tactgatgtt tttaatccga ttgttttaga acaaggaatc ttcacagaaa
    14161 atgatctaag tggaatgttt aacggtggtt tttgggctag taaaaagaat ctgttctcag
    14221 aacaggaatt atatagtgct tttcaagaat gtgccgctca tccagaatat tttgattttt
    14281 ctcaaaaaac ctccgatcaa ccgattatta attataccat tctcaaacga gttcccaatc
    14341 gatttaatat tgtccgcgct ccgggttgtc aagcgggaaa ttggggggga agttctcatt
    14401 ttcaacctca ggggaatatt ttaatcgatc cgagattaaa tcagccctta aaatatttac
    14461 attgggcagg aattcgcatt gaaccggggt gtccctactg ggatatctgg aaatattatc
    14521 gttatctaga tgatcctaat ccccccgcag atccgccagc aagcaaacca aaaaatcctt
    14581 ttcagagatt attagataaa atcaaattat aggtttgatt tttcctctcg caccattaag
    14641 ataactgctg caatttaact ccaatcctgt tgctcctcgg tttggcgaat ttgatacact
    14701 tgtccgtgac tgtgcagttg tcgagttttt gcccataaac tggcttcatc taactgccat
    14761 ttttctagga ttaaatctaa atctctttgg atttctcttg cccccggaaa gttttgataa
    14821 cgtatgcgta aacgagcgca ttcagctaag tttaaatcat tgggttctga ttgcagcagc
    14881 tgatcaacaa tcgggcgatc aagtctttcc tgtggatgtt gttgttcttg atttttacta
    14941 gccataatta atcgaaccaa ccgatatcgg aatccgttaa ctctgtgggg gtaggattgg
    15001 gggcaatata acgggctaaa gtggccattt caaagttata aagactgggt ttcttaggca
    15061 atagtttggt aattgctgac ggttcagggg tggcggtggg actattttct agggttttaa
    15121 tttgattata taatttttca attaactgtt ttttctcggt taattccgtc tgggattgac
    15181 ttaattgact cgataatttt tcaattaact gttttttctc ggttaattcc gtctgggatt
    15241 gacttaattg gctcgataat tttgccacca aagtggtttt ttccgagagg acagattcta
    15301 actgctcggt ctgttgttgt aactgctgta aactgacaga gagttccaga tgttctttaa
    15361 gttcttcttc cagagaaata actttttctt ggagagtttg cccatttttc tcggtttttt
    15421 ctaactctgc cgtcaagtcc ttaatttttg cctgtagggt ggcgttagtt tccttcggac
    15481 gtttggcaga aggagagggt tgggattcta cctctttatt agcttctgta cggatgagat
    15541 cgtttaaatt acctttggtc ataagctctg caaataggat attacgatag agtcggatac
    15601 tgtccagaaa tctgttggtt tagtaggcaa tcgatcatta ttcgcccgat atacaagctt
    15661 gattacgctg gggaattgtt ccataggttt tattgtatag cttgaggtcg atcgatccct
    15721 gtccatttaa ggaatgagaa aaaataccta aatctcagtt tcgccagtaa gattctaaat
    15781 ttttctgtag ttaatgatac ggaatactaa atttgtgtca gactaagggt agaagttgat
    15841 cgcacctgtg gcaaggataa atatatgaat attgaagaaa aagcacgtct taatctaacc
    15901 caagaacgac aagcagaagg aatgctcgaa gaaaatgtct taactcgtac agaggagctt
    15961 ttaggggaag gggaggcagg aactgcgaaa gcacgggttt tattaactga aaatcgtcaa
    16021 gaagacgaaa aactccaaga taagatttta gaacgttcga tcgaagctat gcaatagcct
    16081 ttcggtgagg actgggaata tccacaaaag tatagataaa tatctgtaga gacgtggtta
    16141 actgcgtctc tatttcaatt cccgataact aatcactgtt aaactaatca ctgttcacac
    16201 tctggacagt gaaaaaatgc agatccgaag aaaacacccc aatcctgccg ttaaagttga
    16261 gagtttgagt tatgtggtga aagtgccaga ggcacaaccg caaaatattt tagaagaaat
    16321 cgtttggcat aaggagatag aagtcgataa attgcgcgaa cggttgcctt tattagaatt
    16381 acgccaaaaa atagccaata ctgcgccacc ttgcgacttt ttagccgcct taaaacaggg
    16441 taaaacccaa cccgctttga ttgccgaagt gaaaaaagct tcccctagca agggagttat
    16501 cctggaagat tttgatcctg tagcgatcgc ccgtacctat gaacagggag gggccacttg
    16561 tttatcggtt ttaaccgata gtaagttttt tcaaggtagt tacgaaaatc ttagcctcgt
    16621 ccggcaagcg gtatctttac ccctattatg caaggaattt atcctttatc cctatcaaat
    16681 ctactacgct cgctctaaag gagccgatgc cgtcctttta attgccgcta ttttaagcga
    16741 tcaagactta gcatattttg tcaaaattgt caagggttta gggatgacag ctttggtaga
    16801 ggtgcatagt ctggccgaat tcgatcgagt gttagccatt gagggaatcg aactaatcgg
    16861 cattaataac cgcaatttag agacatttac agtcgattta gacaatactc gccaattact
    16921 agaagccagg ggcgaacagg tgcgagaaaa aggtattctg atcgtcagcg aatcgggatt
    16981 acacacagca acagatctgg cgaaagtgaa acaagcggga gcaaatgcgg ttctaattgg
    17041 cgaatctttg gtaaaactgc ccgatcctgc cctcggtatc caaaaactct ttgaaaatcg
    17101 ggaataggag ttaggaagtg gggaagtggg gaattgggga agctgtctca tcaccctatc
    17161 accccaaaac cccatcaccc caaaacccta tcaccctatc tcctgaatac tgactcctga
    17221 caaggctatc tgccgggata taatcagagt ttgccgaatc ttaaatcatc ccctcagaat
    17281 tgatgcgatc gagatttggt tttttatctt tttgaatata gcccaacgag agaggacgaa
    17341 ccatggaaaa taaaaatatg ttgatgattc ccggccccac accagtgccg gaagcggtat
    17401 tattggctat ggctaaagca ccgataggtc atcggacggg tgcttttagc aaggtaatcg
    17461 ccgaattaac cgaaaatctt aaatggttgc atcaaaccag tggcgatgtc ttaatgctaa
    17521 ccgcttcggg aaccggtgtc atggaagcgg gaattattaa ttttctcagc cccggcgatc
    17581 gagttttggt gggggataat ggtaaatttg gggaacgttg ggcgaaagtc gcgaaagcct
    17641 acggcttaaa tgtggatgta gttaaggcag aatggggtaa acccctagat ccagaagcat
    17701 ttaccgctaa actgaccgaa gacaaggaaa aagccatcaa agcggtaatt atcacccatt
    17761 cggaaacttc caccggggtt ctcaatgact tggaaagcat taacaaagcg gtaaaagacc
    17821 acggggaagc tttaattatc gtcgatgccg tcaccagttt aggagcttat catattccca
    17881 tcgatgaatg gggtttagat gtggtcggct ctggttccca gaaaggttat atgattcctc
    17941 ccggtttagg ttttgtttcc gtaagtcaaa aagcttggaa agcctacgaa accgccaaac
    18001 taccccgttt ttatctagat ttgggcaaat acaaaaaagc caccgatgag gatagttccc
    18061 cttttacccc tccagttaac ctgatgtacg gtttacaagc ttctctgcaa atgatgagaa
    18121 gggagggatt agcggcaatt ttcgcccgtc accaacgctt aacgaagatg acgcgggccg
    18181 gtatgcaggc cctgaatttg cccctctacg cggcggattc ctgcgcttcc acggcgatta
    18241 ctgcggtggc ccccactctg gtggatgcgg aaaaaattcg ttcgactctc aataaaaagt
    18301 tcgacatcgc tctcgctggt ggtcaggatc acctcaaggg taaaatcttc cgtatcggtc
    18361 atttaggttt tgccagtgag agggatatcc tcacggttat cgctgccatt gaagcgactt
    18421 taatcgagtt aggtgtcgaa ggtgtcaccc ccggcgccgg tgtggctgcc gctgcctcgg
    18481 tgatggtgca gggttaatct taggttggct tttccccttc tccccctatc cattagtcac
    18541 atatttctta acataaaatt tgacaaagag agaaaagtgt gcaaaatggc tcaagggggt
    18601 tctatagggg aactaatttg atcagatagt ttccaagtct ggctgatatc ctgtcaactt
    18661 cctagtccca accaaatagt tttaacacca agttcaccta cagatgcgat cgcaaccatt
    18721 cctctaaatc tcccggtttg gtaaaatcta acaaagcttc tcctaaagct tccagttgat
    18781 taattgagag agcttgaatc tgcgatcgct gttcaggagt cacttcaccg atccgacgcg
    18841 agaggagccg cagaatgagc gagcattccc cttcttgacg aaattcttct cgccactcct
    18901 gataaataac agactgttgc ataatgtctc tccgtagaac ctgattgatc aattctttgt
    18961 ttaataataa ccccgccaga atcccagtgg atgccgccac attgctctgt attctctggt
    19021 cgggaatcgc ttctattcta gtagctatct gacgcaaggt ttgggcggtg tcaggagttt
    19081 gggtcaacac agccaagggt aataatcctg tcgcttccag aaaggtttga gttggttgtt
    19141 cccatagtcg aatcacttca aattcgtgac gagtccccgg aatttcaaac gtagtctgat
    19201 gaaccagtgg agatgtggaa cgagtcaaat aaataactac ttggtgcatt cgtttttggg
    19261 gaaaacggcg gaaaccccgc agacgataat ctgccattct gaaaggcatt atgggatcag
    19321 gttctgtttg aaactcgatg tgaagaacat cactatcaga attgagcaaa atcaaagcat
    19381 ccgctcgaat cggctccaag gatagttccg agggactcag ttgggtcatg gtgatgggtt
    19441 cacccagcaa ccaagaggca aaatcttcag aaaagttttc tgcgaggaac ttgcaggtat
    19501 tatcgaacat aaaacagatt ttagagagta atcatctgag ccattcagcc tcaagatgcg
    19561 atcgcaacca ttcctctaaa tctcccggtt tggtaaaatc taacaaagct tctcctaaag
    19621 cttccagttg attaattgag agagcttgaa tttgcgatcg ctgttcagga gtcacttcac
    19681 cgatccgacg cgagaggagc cgcaggatga gcgcagattc tccttcctga cgaccttcct
    19741 ccttggcttc acgatatacc cttgtatctg ccaattgtag tcctagcatt gtatcaacct
    19801 cctgccgact caagttagta aacttataca ccataattgt ggcaatcatt tctattatgg
    19861 cgcgactggc ttctgtatct acaatttgct cttgggttcg tgccagtaaa tagcgggctt
    19921 tttctggtgc ttgggtttct tccacagtag tgagtaccat caaggcaacc cctaggggta
    19981 actgctgaat ttcccctaat tcgttcagat aaactcgatg tacttgctca caattgagaa
    20041 gcgatcggta gggatgggtg ttactttgtt ctagactacg agttggatag atgacaacaa
    20101 tttgccaatc ggtaaatctt tctcgctggc ggtagaaata taaaaatgat tcaccaaaaa
    20161 cccgctcata gagtcgttcg tctttttgaa attgtacttc acaaaaataa atcaccccgt
    20221 tcctatcagt atcaggtggg agaaatacgc catcgatctc aaatttgggt tctttgacgg
    20281 ctacggagtc aaagcggtat tcggtggcat tatcgggtgg gttttccagt aaatcgaaga
    20341 gaagggaggg ggattgttgg aaaagccggt aaaatatcga gtctcgtttc attatgatcg
    20401 ggattgcacc atagcgatct tcccataaaa cccccaaaaa atagagagag gaaacccgcg
    20461 tctcctctcc cttggcacat tattttatgg ccttgcggac agcatcactg ttattcgcaa
    20521 aatagtttga gaaaatcggt agcgcgatag ataggaattc cctcgtaggc gatcatatct
    20581 aacagatcac tgtccccagc gattatgcag tctgcctttc ccgcaacagc gagggagaga
    20641 aatttgtcgt cctttggatc gcggcaagcg atgacagact cggtgacggt gacagattct
    20701 gaaatgccaa cataccactc gatataatcc atggcgctgt cttgtcggat atatttgcga
    20761 aatttttctc tgtttagtac ggtgaatagt tcggcaactg tatcatcgga aatcaaggta
    20821 ataccttgtc tcgccaaaaa attgaacatt tgctcaaaat tttcgttcgg acggatggcg
    20881 aaactgacaa gaatattggt atcaacaaca acccgcatca ccaatcattt tttaacgatg
    20941 ccatgagtgc gtcgtgttcc gcagctgtca tgccaagagt tgcggcctcg tgctgtaatt
    21001 ttctggcaat aatagataag ctgggacgac ccaccataag gcggacaatt tcatcatctg
    21061 gtataagcgc cagtgcttct ctaatttctt tggctgtgcc ttggattact gttgcttgtg
    21121 tagtcatgat ttttgatcat tctagagcta atcctagcat aatttagatc aaaaaacctc
    21181 ccccaaaaca agagagagga aacctgtatt ctagaaaacg ggtttctcaa agaaacccgt
    21241 tttctatggc gcttaagtgt cgctgccgcc cccagtaagt ctaacagcac ctcctaacat
    21301 taagccgccc agggtgatca aaccgagaag gctagagggt tcggggacac tgactgctgg
    21361 agtcggcact gtcaggactg aggacattcc atatagagtg tatgagttac ttgaactaag
    21421 accgatcata ttgatccacg aggcgttagt ttgaacaggg ctacctatag taaccatagg
    21481 cggaaaaccc gagttaccca acggggattg agcggagcca cccacattag tgcctgtcca
    21541 tacagaattt gttccagatg tgtctatctg agtgcgatta ataaaggttt ggatgctgcc
    21601 atcccaaaga tcggcattat tattggcgac cttgttgcca tctatgaggt agatgggaac
    21661 atctggctga ctgccatcag taccggtatt gactttggcc gaggtagcgg aagtagaacc
    21721 gatcgccttc caagtgattg tacccaggtt aaatcctgcc gttgttaatt gcgtagcaag
    21781 agcgcttcct gttacctggt tggtaacaaa gttattgtag tcgttgatgt tggtgcttgt
    21841 tgcatcccgt gtgccgtcag tgacaaagac caagcgatac tggtcgcccg gattcaagcc
    21901 ggggggta
//
