LOCUS       NZ_AP024135            11320 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces sp. EAS-AB2608 strain NBRC 114648 chromosome.
ACCESSION   NZ_AP024135
VERSION     NZ_AP024135.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. EAS-AB2608
  ORGANISM  Streptomyces sp. EAS-AB2608
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Okubo,S., Ena,E., Okuda,A., Kozone,I., Hashimoto,J., Nishitsuji,Y.,
            Fujie,M., Satoh,N., Ikeda,H. and Shin-Ya,K.
  TITLE     Identification of functional cytochrome P450 and ferredoxin from
            Streptomyces sp. EAS-AB2608 by transcriptional analysis and their
            heterologous expression
  JOURNAL   Appl Microbiol Biotechnol 105 (10), 4177-4187 (2021)
   PUBMED   33944982
  REMARK    DOI:10.1007/s00253-021-11304-z
REFERENCE   2  (bases 1 to 9320785)
  AUTHORS   Okubo,S., Ena,E., Okuda,A., Kozone,I., Hashimoto,J., Nishitsuji,Y.,
            Fujie,M., Satoh,N., Ikeda,H. and Shinya,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Contact:Shinya Okubo Eisai Co., Ltd.;
            Tokodai 5-1-3, Tsukuba 300-2635, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Coverage                   :: 53x
            Sequencing Technology             :: Miseq; PacBio RS II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703895.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 09:16:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,174
            CDSs (total)                      :: 8,084
            Genes (coding)                    :: 7,890
            CDSs (with protein)               :: 7,890
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 87 of 194
            Pseudo Genes (incomplete)         :: 132 of 194
            Pseudo Genes (internal stop)      :: 10 of 194
            Pseudo Genes (multiple problems)  :: 32 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:41:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6132416
            Orig. end                         :: 6143736
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024135.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11320
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11320
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11320
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6320](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6320)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    122..140
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            659..1390
                     /gene="hemQ"
                     /locus_tag="K6K39_RS27015"
                     /old_locus_tag="EASAB2608_05528"
     CDS             659..1390
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="K6K39_RS27015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_05528"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_059253495.1"
                     /transl_table=11
                     /translation="MSDDAPTTESGRIPNKGKLAKDLNEVIRYTLWSVFRLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTVRGTYDVSGLRADADLMIWWHAETSDQLQEAYNLFRRTR
                     LGRALTPVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRGSEARMHVR
                     EEVPFFTGRRKDIADLVAGLA"
     gene            complement(1412..3058)
                     /locus_tag="K6K39_RS27020"
                     /old_locus_tag="EASAB2608_05529"
     CDS             complement(1412..3058)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (117-490). E-value:
                     5.2e-20. Score: 64.0. Matches aSDomain:
                     nrpspksdomains_K6K39_RS27020_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675655.1"
                     /locus_tag="K6K39_RS27020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_05529"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_221334973.1"
                     /sec_met_domain="PF00561 (E-value: 2.6e-23, bitscore: 81.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAAALYSAAGSLLLTTLAAAPAGGAPGTAGVPGVPGTAELRGTA
                     VAVARARAAGVDFGPCSAADVPEAPAGVRCGTVAVPLDYAHPYGEQIRLTVSRARATHK
                     DPHNSKRKIPRQGALLYNPGGPGASGMYFPLIGTVPEWKRIAAAYDLVGYAPRGVGRSA
                     PLSCQDPKTFGTAPTASPTHPSEAYKRQRIAQAKAYARGCAERSGSRLRFYHSLNNARD
                     LDVLRAALGEDRLTFVGASYGTYFGALYAAMFPSHVRRMVLDAAVNPDPQRIWYRSNLD
                     QSVAFEERWADFREWIARHDDVYRLGATPARVQRSYDTARERLARKAAGGKVGPGQLQN
                     AFLTAGYYDDYWPSRAAALSAYLHGDPEPLVRLAAPRPGAAVAAENGSAVYTAVECNDA
                     PWPTDWKVWDRDNTRLARVAPFETWDNAWMNLPCAYWPAPRQQPLDVRTGPGELPPVLI
                     LAAERDAAAPYQGALEMNRRLAGSVLVTERDAGTHGIAGGPNACVNGYLDAYLLEGRVP
                     ARHASCAPRRLPAAVRPEAQRDALKGKAERR"
     aSDomain        complement(1589..2707)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K6K39_RS27020_Abhydrolase_1.1"
                     /evalue="5.20E-20"
                     /label="K6K39_RS27020_Abhydrolase_1.1"
                     /locus_tag="K6K39_RS27020"
                     /protein_end="490"
                     /protein_start="117"
                     /score="64.0"
                     /tool="antismash"
                     /translation="ALLYNPGGPGASGMYFPLIGTVPEWKRIAAAYDLVGYAPRGVGRS
                     APLSCQDPKTFGTAPTASPTHPSEAYKRQRIAQAKAYARGCAERSGSRLRFYHSLNNAR
                     DLDVLRAALGEDRLTFVGASYGTYFGALYAAMFPSHVRRMVLDAAVNPDPQRIWYRSNL
                     DQSVAFEERWADFREWIARHDDVYRLGATPARVQRSYDTARERLARKAAGGKVGPGQLQ
                     NAFLTAGYYDDYWPSRAAALSAYLHGDPEPLVRLAAPRPGAAVAAENGSAVYTAVECND
                     APWPTDWKVWDRDNTRLARVAPFETWDNAWMNLPCAYWPAPRQQPLDVRTGPGELPPVL
                     ILAAERDAAAPYQGALEMNRRLAGSVLVTERDA"
     gene            complement(3249..4028)
                     /locus_tag="K6K39_RS27025"
                     /old_locus_tag="EASAB2608_05530"
     CDS             complement(3249..4028)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675656.1"
                     /locus_tag="K6K39_RS27025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_05530"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_221334974.1"
                     /transl_table=11
                     /translation="MFWVLLLLLAWAFAGTACTRLCLAAVRAAAVDARDAPAAQERDLT
                     LYEAAFLSGGPARVADVTLVAMARQRRLLLAHTGWATVVDPRGRDEMERSVIGAIGPEG
                     QSQIAPVRARAAGAEAVRRLADGLVRAGLAVPEGAATTVGAAVRQVRAAALAVAVLGTT
                     ALLLPVQTGTPRLLVAAWFALPLTLALSCLAIARFEVHPYSRWASPAGQRLLSALAGRP
                     DGDERSFLTSVAVRGIRAIGEPELRAAFTHRDQPWRE"
     misc_feature    4089..4106
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 13.84"
                     /tool="antismash"
     gene            complement(4145..4894)
                     /locus_tag="K6K39_RS27030"
                     /old_locus_tag="EASAB2608_05531"
     CDS             complement(4145..4894)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125239.1"
                     /locus_tag="K6K39_RS27030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_05531"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_221334975.1"
                     /transl_table=11
                     /translation="MRPRPPIDGRGIFSGTGLVITALAATVVALLVPLWSYADRQDPAS
                     ASVLSAQTVTTPYGPLSAQDRDFVAKVRLAGLWELPAGELALRKGTTPAVRTAGRHLVD
                     GHTSLDAHARTVATQLGVALSNEPNEQQKQWLAALNAAEGQDFDRRFAGILRLAHGRVF
                     SLVAQVRAGTQNSLVRDLADNANATVLDHIKVLEATGYVDFAELAEELAASPSPVPAPP
                     EVDPGAAVPVTPSPGGTSSPPPATSSP"
     gene            complement(5001..6320)
                     /locus_tag="K6K39_RS27035"
                     /old_locus_tag="EASAB2608_05532"
     CDS             complement(5001..6320)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125240.1"
                     /locus_tag="K6K39_RS27035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_05532"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_221334976.1"
                     /sec_met_domain="DUF692 (E-value: 7.5e-95, bitscore: 316.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLGTGIGWRPEIADAVERMRGIDWVEVVAENVCPGHLPESLLR
                     LRERGVTVIPHGVSLGLGGADRPDEGRLTALAERAEALGAPLVTEHIAFVRAGGALTAS
                     PRLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALFSWPDEELTEGQFLS
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAEALAALPLEALAYVHVAGGFERDGVWHDSH
                     AHRVPRPVLDILTGLAARVAPPGVLLERDENFPDPAELVRELTAIRGAVAAGRSHAARG
                     TAARGTAVAGAAGAGAGGVGVVARPVAGVRERVAVAQTAVLSSLVAGTPVPEGFDRVRM
                     GVQARALAAKRAGVVAKVAPELPVILGAGYREAFLAYAQQRPMRGGYRQDALDFAGHLL
                     KLGRPAEPGARRELRDWWLDRAGPAPRKRGLVSRVLRRRG"
     gene            complement(6533..7273)
                     /locus_tag="K6K39_RS27040"
                     /old_locus_tag="EASAB2608_05533"
     CDS             complement(6533..7273)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030616.1"
                     /locus_tag="K6K39_RS27040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_05533"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_221334977.1"
                     /transl_table=11
                     /translation="MSHDPAPASDSPFRTEPDPRDEAPQFVLPLVVRLERSAPPARTDA
                     LETAARAVLVLLGDERAQGDGEWAEAVRSWEDARIRKVVRRARGAEWRRAEALPGITVT
                     GKSAEVRVYPPIPLDGWPKELAKLQVSGTELDDPEPPLPAHPADGPVLWLNPDLDMSAG
                     KAMAQAGHGAQLAWWALPEPARTAWRDAGYALAVRTADPSDWPRLTASGLPVVRDAGFT
                     EIAPGSCTVVADHPALHRAARPAH"
     gene            7408..7596
                     /locus_tag="K6K39_RS27045"
                     /old_locus_tag="EASAB2608_05534"
     CDS             7408..7596
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675660.1"
                     /locus_tag="K6K39_RS27045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_05534"
                     /product="hypothetical protein"
                     /protein_id="WP_221334978.1"
                     /transl_table=11
                     /translation="MSRYADVGDETLWNPSDGEALPDGPRTAVQGPAAGVSGPGDGGPR
                     AAVPRARAAGPSRGTAR"
     gene            7638..8513
                     /locus_tag="K6K39_RS27050"
                     /old_locus_tag="EASAB2608_05535"
     CDS             7638..8513
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675661.1"
                     /locus_tag="K6K39_RS27050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_05535"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_059255309.1"
                     /transl_table=11
                     /translation="MHRDLRLTAVGRRYGIRGPWVLRGVDLTVAPGTLTRIEGANGSGK
                     STLLRLLARLDAPTEGRITGRPRTAYVPERFPSALPFTAAGYLTHLGTVHGLGRTAAAR
                     AAHTWLERFGAGAYATTPMARLSKGSSQKVAVAQALLGDPELLVLDEAWTGLDAAARAE
                     LERAVAERTAAGGAVVFVDHDPRRLAGAPDATYTVTDGALRRRSERGGTADGPVTLVVA
                     HGPAGVPLPPDAARAVVSAEETSPGRHRLAVLASHSDVLLRALLTARPPWHVVRVEPAG
                     ASPPDPGSRP"
     gene            8510..9181
                     /locus_tag="K6K39_RS27055"
                     /old_locus_tag="EASAB2608_05536"
     CDS             8510..9181
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997023.1"
                     /locus_tag="K6K39_RS27055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_05536"
                     /product="ABC transporter"
                     /protein_id="WP_086697846.1"
                     /transl_table=11
                     /translation="MTALLRYQADLLLRSQRWLPPVILYAVFLGVGVQSGQPVLDSLGY
                     TAAALLPVSAWLVRICATGEPPAARACVAAARGPARAHLACLLTALLAAVLLGVAATVV
                     VTLISDPWSNGHRVRVPPPRALAAGLPAALACALLGTAVGALTNRPVLRSTGRAVPAML
                     LGALLAMVLAGSPAQAAVSGLVSGSQTGRAPLPLLPLAGAALVTAAAFAVAARLAARRS
                     P"
     gene            complement(9173..9355)
                     /locus_tag="K6K39_RS27060"
                     /old_locus_tag="EASAB2608_05537"
     CDS             complement(9173..9355)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675663.1"
                     /locus_tag="K6K39_RS27060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_05537"
                     /product="hypothetical protein"
                     /protein_id="WP_059253513.1"
                     /transl_table=11
                     /translation="MSDEDYCTITDAVKPAKADGPPYAECVLCREPTEYPESYKGVTLC
                     PVCEWREAERTSCSG"
     gene            complement(9374..10219)
                     /locus_tag="K6K39_RS27065"
                     /old_locus_tag="EASAB2608_05538"
     CDS             complement(9374..10219)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675664.1"
                     /locus_tag="K6K39_RS27065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_05538"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_059253515.1"
                     /transl_table=11
                     /translation="MIPRVRRIAAVCVLGALLTACGTAGGDRPAPAASPASGSPSSARP
                     PVLAPGPGGLTPVFEHGPRTRGKTVALTFDADMTADQGPRAASGERFDNPGLIGALRAL
                     KVPATVFMTGRWAEQYPAEARDLGRDPQFEVANHSWSHHAFTADCYGLPTVPADGMRAD
                     VQRAYTSLREAGVPDPMPYFRFPGGCYDERALRALSGLGVTAVQWDVVSGDAFATDADA
                     VVRQVLDGVKPGSVVVLHCTRSAAPTTERVVRTVVPELRERGYRFVKVSELIGQTGAGR
                     "
     misc_feature    complement(10237..10251)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.84"
                     /tool="antismash"
ORIGIN
        1 gcgtgctgca cgccgacgcc gtgatcgtcg ccgtacccgc cccggccgcc gccgggctgc
       61 tgcgcgccga gtcccccgcg gccgccgccg agctgtccgc cgtggagtac gcctcgatgg
      121 cgctggtcag cctcgcctac cggcgcgccg acctgagcct gcccgcgggc agcggcttcc
      181 tcgtgccgcc cgtcgacggc cgcaccatca aggccgccac cttcgcctcc cagaagtggg
      241 gctggatcgc cgaggaggac ccggacgtgg tggtgctgcg cacctccgtg ggccgctacg
      301 gcgagacggc ggtgctcggc cgggacgacg ccgacctggt ggagatctcc cggcacgacc
      361 tgcgggaggc caccggcctg gacgccgtac cgctggagac ccgggtcacc cgctggaccg
      421 acggcctgcc ccagtacccg gtggggcacc acgcgcgcgt ggcccgcgtc cgggagcgga
      481 tcgcggcgct tccgggactc gcggtgtgcg gcgcgcagta cgacggcgtc ggcatccccg
      541 cgtgcatcgc gagcgcgtac gcggcggtgg accagctgga cggcgacccc gccggggtcg
      601 cggagctgac ggcgaacccg gtgcagagcc tgcacggcgg agcgggagaa tagggaccat
      661 gagtgacgac gcccccacca ccgagtccgg caggatcccg aacaagggca agctggccaa
      721 ggacctcaac gaggtcatcc gctacaccct ctggtcggtc ttccggctga aggacgtgct
      781 gccggaggac cgcgccggat acgccgacga ggtccaggag ctgttcgacc agctcgccgc
      841 gaaggacgtg accgtccgcg gcacctacga cgtctccggc ctgcgcgccg acgccgacct
      901 gatgatctgg tggcacgccg agaccagcga ccagctccag gaggcgtaca acctcttccg
      961 ccgcaccagg ctgggccgcg cgctcacgcc cgtgtggtcg aacatggcgc tgcaccgccc
     1021 ggccgagttc aaccgctcgc acatcccggc gttcctcgcc gacgagacgc cccgcgacta
     1081 cgtgagcgtg taccccttcg tccgctccta cgactggtac ctgctgccgg acgaggaccg
     1141 ccgccgcatg ctcgccgacc acggcaagat ggcccgcggc taccccgacg tgcgcgccaa
     1201 cacggtcgcc tcgttctccc tcggcgacta cgagtggatc ctggccttcg aggccgacga
     1261 gctgtaccgc atcgtcgacc tcatgcgcca cctgcgcggc tccgaggccc ggatgcacgt
     1321 ccgcgaggag gtcccgttct tcacgggccg ccgcaaggac atcgcggacc tggtggcggg
     1381 cctggcctga gccgcgcccc cgcaggcggc cctaccggcg ctcggccttc cccttgaggg
     1441 cgtcccgctg agcctccggc cggaccgccg caggcaaccg gcgcggcgcg cacgacgcgt
     1501 gccgcgccgg gacccggccc tccagcagat acgcgtccag gtatccgttg acgcacgcgt
     1561 tgggcccgcc cgcgatgccg tgcgtccccg cgtcccgctc ggtcaccagc accgagccgg
     1621 cgagccgccg gttcatctcc agggcgccct ggtagggcgc ggcggcgtcc cgctcggccg
     1681 ccaggatcag caccggcggc agctcaccgg gcccggtccg cacgtccagc ggctgctgcc
     1741 ggggcgccgg ccagtaggcg cacggcaggt tcatccacgc gttgtcccag gtctcgaagg
     1801 gcgccacccg ggcgagccgg gtgttgtcgc ggtcccacac cttccagtcc gtcggccagg
     1861 gcgcgtcgtt gcactccacg gccgtgtaca ccgcgctgcc gttctccgcc gcgacggccg
     1921 cccccgggcg gggcgcggcc agccggacca gcggctccgg gtcgccgtgc agatacgccg
     1981 agagggccgc ggcccggctc ggccagtagt cgtcgtagta cccggccgtc aggaacgcgt
     2041 tctgcaactg gccggggccc accttcccgc ccgccgcctt cctcgccagc cgctcgcgcg
     2101 cggtgtcgta actgcgctgc acccgcgcgg gcgtggcacc gagccggtac acgtcgtcgt
     2161 gccgggcgat ccactcccgg aagtcggccc agcgctcctc gaacgccacc gactggtcca
     2221 ggttgctgcg gtaccagatc cgctgcgggt ccgggttcac cgccgcgtcc agcaccatgc
     2281 gccgcacgtg cgaggggaac atcgccgcgt acagcgcgcc gaagtaggtg ccgtacgacg
     2341 cccccacgaa ggtgagccgg tcctcgccca gtgccgcccg caggacgtcc aggtcccggg
     2401 cgttgttgag cgagtggtag aaccggagcc tgctgccgga ccgctcggcg cagccgcgcg
     2461 cgtacgcctt cgcctgggcg atgcgctgcc gcttgtacgc ctccgagggg tgcgtcggtg
     2521 acgcggtggg cgcggtgccg aaggtcttcg ggtcctggca ggacagcggc gccgaccggc
     2581 ccaccccgcg cggggcgtag ccgaccaggt cgtaggcggc ggcgatccgc ttccactccg
     2641 ggaccgtgcc gatcagcggg aagtacatgc cggaggcgcc ggggccgccc gggttgtaga
     2701 gcagggcgcc ctgccggggg atcttccgct tgctgttgtg cgggtccttg tgggtggccc
     2761 gggcgcggct gacggtcagc cggatctgtt cgccgtacgg gtgggcgtag tccaggggga
     2821 ccgccaccgt gccgcaccgc acgccggccg gcgcctccgg tacgtcggcg gcggagcagg
     2881 ggccgaagtc gacaccggcg gcccgggcgc gggccacggc caccgcggtg ccgcgcagtt
     2941 cggccgtgcc ggggacgccc gggacacccg cagtgccggg ggcgccgccc gccggggccg
     3001 cggccagggt ggtcagcagc aaggaaccgg cggccgagta gagggcggcg gctttcatcg
     3061 ggcatccctt cggtgcgcgg cggcgatggc acggctgcca cggcccggcg ccactacagg
     3121 gatgtttcgg tcagccgtgg ggtgaagcaa gcaccacccg gccggtgtcg gccccaatgc
     3181 ccccgtgcgc cgcccgcgaa gcccccttcg cctccgctca ctcccccttc actcccgctc
     3241 gctcccgttc attcccgcca gggctggtcg cggtgggtga aggcggcgcg cagctccggt
     3301 tcgccgatcg cgcgtatgcc gcggacggcg acggaggtga ggaacgaccg ctcgtcaccg
     3361 tccggccgtc cggccagcgc gctcaacagc cgctgaccgg cgggggaggc ccagcgcgag
     3421 tacggatgca cctcgaagcg ggcgatggcc aggcagctca gcgccagcgt cagcggcagg
     3481 gcgaaccacg cggccaccag caggcgcggc gtcccggtct ggacgggcag cagcagcgcg
     3541 gtcgtgccca ggacggccac ggccagcgcg gcggcccgca cctgccggac ggcggccccc
     3601 acggtcgtcg ccgcaccctc gggcaccgcg agaccggcgc gcaccagccc gtccgcgagc
     3661 cggcgcacgg cctccgcgcc ggccgcccgc gcccgcacgg gcgcgatctg cgactgcccc
     3721 tcgggcccta tggcccctat gacggaccgc tccatctcgt cgcggccccg cgggtccacg
     3781 acggtggccc agccggtgtg cgcgagcagc agccggcgct ggcgggccat ggcgaccagg
     3841 gtcacgtcgg cgacccgcgc ggggccgccg gagaggaagg ccgcctcgta cagcgtcaga
     3901 tcgcgctcct gggccgccgg tgcgtcccgc gcgtccacgg ccgccgcgcg cacggccgcc
     3961 aggcacagcc gggtgcaggc cgtaccggcg aacgcccagg ccagaagcag gagtaggacc
     4021 cagaacatga cgtgtttgta tgccagagga ctgggcggac gccatgccct gttcacgatc
     4081 cggacggagt gttgcgggta tgtgaccttc cgtttcccgc cctggtcagg ggtgccaggc
     4141 ggcctcaggg gctgctcgtc gccggcggcg gggaggacgt cccgcccggc gacggcgtga
     4201 cggggacggc ggcgcccggg tcgacctccg gcggggccgg caccggggag ggggaggccg
     4261 ccaactcctc ggccagctcg gcgaagtcga cgtacccggt ggcctccagg accttgatgt
     4321 ggtccaggac ggtcgcgttg gcgttgtcgg cgagatcgcg gaccagggag ttctgggtgc
     4381 cggcccggac ctgggcgacc agggagaaga cccggccgtg cgcgagccgc aggatgccgg
     4441 cgaaccggcg gtcgaagtcc tggccctcgg ccgcgttcag ggcggccagc cactgcttct
     4501 gctgctcgtt cggctcgttg gacagggcca cgccgagctg ggtggcgacg gtccgggcgt
     4561 gcgcgtccag ggaggtgtgg ccgtcgacga ggtgccggcc ggccgtgcgg accgccggtg
     4621 tggtcccctt gcgcagcgcc agctccccgg cgggcagttc ccacagcccg gccagccgga
     4681 ccttggcgac gaagtcgcgg tcctgcgcgg acagcgggcc gtacggggtc gtcaccgtct
     4741 gcgcgctcag cacgctcgcg ctcgccggat cctgccggtc ggcgtaggac cagagcggga
     4801 cgagcagcgc gacgaccgtg gccgccagag cggtgatgac gagaccggtg ccgctgaata
     4861 tgcctcggcc gtcgatcggg ggtcgcggtc gcatggtgcc tcctgcaccg cacgccgggg
     4921 tgtgctgggg gtgggtgcgg ggttggcata ttactggggc gccgggaccg gcccgcgggt
     4981 gcggggtgcg gggcggccgt tcagccccgg cggcgcagca cccgcgacac cagcccccgc
     5041 ttccgggggg ccgggcccgc ccggtccagc caccagtccc gcagttcccg ccgcgccccc
     5101 ggctccgccg gccggcccag cttcagcagg tgcccggcga agtccagggc gtcctgccgg
     5161 tacccgcccc gcatcggacg ctgctgggcg tacgccagga acgcctcccg gtaccccgcc
     5221 cccaggatca ccggcagctc gggcgccacc ttggccacca cacccgcccg cttcgccgcg
     5281 agcgcccgcg cctgcacgcc catccgcacc cggtcgaacc cctccggcac cggcgtcccc
     5341 gccaccaggg acgacagcac ggcggtctgg gcgaccgcga cccgctcgcg caccccggcc
     5401 accggccgcg cgacgacacc gacacccccg gccccggccc cggcggcgcc cgccaccgcc
     5461 gtacccctgg ccgccgtacc gcgtgccgcg tgcgaccggc cggccgccac ggctccccgg
     5521 atcgcggtca actcgcgcac cagctcggcg gggtcgggga agttctcgtc ccgttccagg
     5581 agtacgccgg gcggtgcgac gcgggcggcc aggccggtca ggatgtcgag gaccgggcgc
     5641 ggcacccggt gggcgtggct gtcgtgccag acgccgtcgc gctcgaagcc gcccgcgaca
     5701 tggacgtagg cgagcgcctc cagcggcagc gcggccaggg cctcggccgg gtcctcgccc
     5761 cggttgacgt ggttggtgtg caggttggcg acgtcgatca gcagccgtac gccggtccgc
     5821 tcgaccagct cggacaggaa ctgcccctcc gtcagttcct cgtccggcca ggagaacagc
     5881 gcggcgatgt tctccagcgc cagcggcacc ggcagcgcgt cctgggcgat gcggacgttc
     5941 tcgcacagca cgtccagcgc gtcccgggtg cgcggcacgg gcagcagatg gccggcctcc
     6001 agccgcgggg aggcggtgag cgcgccgccc gcccggacga aggcgatgtg ctcggtgacc
     6061 agcggcgccc cgagggcctc cgcccgctcg gcgagcgccg tcagccgccc ctcgtcgggg
     6121 cggtccgcgc cgccgaggcc gagggagacg ccgtgcggga tcaccgtcac accgcgctcg
     6181 cgcagccgca gcagcgactc ggggaggtgg ccggggcaga cgttctccgc cacgacctcc
     6241 acccagtcga tgccccgcat gcgctccacg gcgtccgcga tctccggacg ccacccgatc
     6301 ccggtcccca gtacccgcat gctcgtcccc ctcctcggcc ggtgtgcgtg cgtcgagtga
     6361 ggggggtatg gccccggcgg acggccgcga atccccgccg tggcgccttc agagcaacat
     6421 ttgaggttcc gccggagcgc accgggcggg ccggggcgag gcaggccgcg accgggtcgg
     6481 cgttcccgcg gcggggccga gggcggccgg ccgcgtaccg gacccggccg ggtcagtgtg
     6541 cgggccgggc cgcccggtgc agcgccgggt ggtccgcgac caccgtgcac gaccccggcg
     6601 cgatctccgt gaaaccggcg tcgcgtacga cgggcaggcc gctcgcggtc agccggggcc
     6661 agtcggacgg gtcggccgtg cggacggcga gggcgtagcc cgcgtcccgc caggcggtgc
     6721 gggccggctc cggcagggcc caccaggcca gttgggcacc gtggccggcc tgggccatcg
     6781 ccttgccggc cgacatgtcc aggtcggggt tgagccagag caccgggccg tcggccgggt
     6841 gggcgggcag gggcggttcg gggtcgtcca gctcggtgcc cgacacctgg agcttcgcca
     6901 gctccttcgg ccagccgtcc agcgggatgg gcgggtagac ccggacctcc gcggacttgc
     6961 cggtcaccgt gatcccgggc agtgcctcgg cgcgccgcca ctcggcgccc cgggcccgcc
     7021 ggaccacctt ccggatccgg gcgtcctccc agctccgcac cgcctcggcc cactcgccgt
     7081 ccccctgcgc ccgctcgtcg ccgagcagca cgagaacggc ccgcgcggcc gtctccagcg
     7141 catcggtacg ggcgggcggc gcgctccgtt ccagccgtac gacgagcggc agcacgaact
     7201 ggggtgcctc gtcgcggggg tcgggttcgg tgcggaaggg gctgtcactg gcgggggcgg
     7261 gatcgtggct cacgggagcc cagcctacga agccgcgaga gggccgatca acgcgcgggc
     7321 cccgcggggg aaaatccggc gacaccggtg aaccggacgg ccacgctccc cctcttgatc
     7381 cactgagggg accgaagggg gagcgcgatg agccggtacg ccgacgtcgg ggacgagacg
     7441 ctgtggaacc cgtccgacgg ggaggccctt ccggacgggc cccggacagc cgtacagggg
     7501 ccggccgccg gggtgtccgg gccgggggac ggcggacccc gggccgccgt gccgcgggcc
     7561 agggcggccg ggccgagccg gggcacggcc cgttgacccg ggcaggggcc gggcccgacc
     7621 gtgccgtcat ggcccccgtg caccgtgacc tgcgcctgac cgccgtgggc cgccgctacg
     7681 gcatccgcgg cccctgggtg ctgcgcggcg tcgatctcac cgtcgccccc ggcaccctca
     7741 cccggatcga aggggcgaac ggcagcggga agtccaccct gctccggctg ctcgcccggc
     7801 tcgacgcgcc caccgagggc cggatcaccg gccgcccgcg caccgcgtac gtgcccgagc
     7861 ggttcccgtc cgccctgccg ttcacggccg ccggctacct cacccacctc ggcaccgtcc
     7921 acggcctcgg ccggacggcc gccgcgcgcg ccgcgcacac ctggctggag cggttcggcg
     7981 cgggcgcgta cgccaccacg ccgatggcgc ggctgtccaa gggcagcagc cagaaggtcg
     8041 ccgtcgccca ggccctgctc ggcgaccccg agctgctggt gctggacgag gcgtggaccg
     8101 gtctcgacgc ggcggcccgg gccgagctgg agcgggcggt ggccgagcgg acggccgccg
     8161 gcggcgccgt ggtcttcgtg gaccacgacc cgcgccggct cgccggggca ccggacgcga
     8221 cgtacacggt gaccgacggc gcgctgcgac gccgcagcga gcgcggcggt acggcggacg
     8281 ggccggtcac cctcgtcgtc gcgcacgggc cggcgggcgt tccgctgccg ccggacgcgg
     8341 cccgggccgt cgtctccgcc gaggagacga gccccggccg ccaccgtctc gccgtcctcg
     8401 cctcccactc cgacgtcctg ctgcgcgccc tgctcacggc ccgcccgccc tggcacgtgg
     8461 tgcgcgtcga acccgccggc gcatcgcccc ccgaccccgg aagccgccca tgaccgcact
     8521 cctgcgctac caggccgacc tgctgctgcg ctcccagcgc tggctgcccc cggtgatcct
     8581 gtacgccgtg ttcctgggcg tcggcgtgca gagcggccag ccggtcctgg actccctcgg
     8641 ctacacggcc gccgcgctgc tgccggtgtc cgcctggctg gtccggatct gcgccaccgg
     8701 cgagcccccc gcggcccggg cctgcgtcgc cgccgcccgc ggccccgccc gggcgcacct
     8761 cgcctgtctg ctgaccgcgc tgctcgcggc cgtgctgctg ggtgtggcgg ccaccgtggt
     8821 ggtgacgctg atcagcgatc cctggagcaa cggccaccgg gtccgcgtcc ccccgccgcg
     8881 cgccctcgcc gccgggctgc cggccgcgct cgcctgcgcc ctgctcggta cggccgtcgg
     8941 cgcgctcacc aaccggccgg tgctgcgctc caccgggcgt gccgtgccgg ccatgctgct
     9001 gggcgcgctg ctcgcgatgg tgctggccgg atccccggcg caggcggccg tcagcggcct
     9061 ggtctccggc tcgcagaccg gccgggcgcc gctgccgctg ctgccgctgg ccggcgcggc
     9121 cctggtgacg gccgcggcct tcgccgtggc ggcccggctc gccgcccgcc gctcaccctg
     9181 agcaggaggt gcgctccgcc tcccgccact cgcacaccgg gcacagcgtg acgcccttgt
     9241 acgactccgg gtactccgtc ggctcccggc acagcacgca ctccgcgtac ggcggtccgt
     9301 cggccttggc cggcttcacc gcgtcggtga tcgtgcagta gtcctcgtcg ctcataccct
     9361 cagcgtagga cggtcagcgg ccggcgccgg tctggccgat cagttcggag accttcacga
     9421 accggtagcc gcgctcgcgc agttccggta cgaccgtgcg gacgacccgc tcggtggtcg
     9481 gggcggcgct gcgggtgcag tgcaggacga ccaccgaacc gggcttcacg ccgtcgagca
     9541 cctgcctgac caccgcgtcg gcgtccgtgg cgaacgcgtc gccgctcacc acgtcccact
     9601 gcaccgcggt caccccgagc ccgctgagcg cgcgcagcgc ccgctcgtcg taacagccgc
     9661 cggggaagcg gaagtacggc atcggatccg gcacgcccgc ctcgcgcagg gaggtgtacg
     9721 cccgctggac gtccgcccgc atcccgtcgg cggggacggt cggcaggcca tagcagtcgg
     9781 cggtgaacgc gtggtggctc caggagtggt tggccacctc gaactgcggg tcgcggccca
     9841 ggtcgcgggc ctcggccggg tactgctcgg cccagcggcc ggtcatgaac acggtcgccg
     9901 gcaccttgag cgcgcgcagg gcgccgatca ggccggggtt gtcgaagcgc tcgccggagg
     9961 ccgcgcgggg tccctggtcg gcggtcatgt cggcgtcgaa ggtgagcgcg accgtcttgc
    10021 cccgggtgcg cgggccgtgc tcgaagaccg gggtcagccc gccggggccc ggcgccagca
    10081 cgggcggacg ggcggaggac ggggagccgg aggcggggga ggcggccggg gccgggcggt
    10141 caccgcccgc ggtaccgcag gcggtgagca gcgcgcccag gacacagacg gcggcgatac
    10201 ggcgtacgcg tgggatcacc ctacgaacgt agagcagaat gtctgattat gtggcgatat
    10261 gactcacggt ctggcggtgc ggtcacccgc gcggcggcgg cctcgcccct cactcctcca
    10321 gctcccgccg ctccaggggt tcgaccgccg gaccctggat caccgagccg tcgaccgcga
    10381 accgcgagcc gtggcagggg cactcccagg cgcgctcggc gcggttgaag gagaccagac
    10441 agccgaggtg ggtgcagcgg gccgacacgg cgtgcagggc accctcctcg tcgcggtgca
    10501 ccgcgagccg ggagccgccg gagcggacca gcgcgccgtc gcccggcgcc agaccggcca
    10561 ccgaggccgg cggcttcagc cggtccccga cgaagtgccg ggccacctcc gcctgcgtct
    10621 tcagcagcga cggcgcctca cgcaccaccg acttcagccg gcgcgggtcg tacagctcgc
    10681 gccacgcgca ctcccggccg gtgatcagcg cggtcagcag cagcccggcc atgaggccgc
    10741 cgctcaggcc ccagccgccg aatcccgcga cgacataggc gtgccgggcg cccgggtgca
    10801 ggggaccgac cagcgggacc gtgtcggtgg actcgttgtc ctgggtggcc caggcgtggt
    10861 ccagccgcac ccccgggaag tgcacctccg cccaggcggc gagacggtcg aagccggcct
    10921 gcggatcgcc ggtgccgggc gtgaagtgct ccccggtgac caccagcagc cgctgcccgt
    10981 ccgcgccgtg gggcgccgtg cgcaccgagc gggtgttctc ctccggtgtg atgtacatgc
    11041 cctcaggatc cggcccgtcg cccagcggcc cggccacgac cagttcccgg cgcggggaca
    11101 gccgggtgaa cagcagggcc cggtcgaaca ccggatagtg ggtggccacc accacgtccc
    11161 gggcggtcac gatggcaccc ttctcggtcg tcagccggca cggctcgccc tccgccagac
    11221 cggtgacccg cgtgtgttcg aacacctggc cgccgtgcgc cagcaggtcg gcggtgagcg
    11281 cccgcaggta cttgacgggg tggaactgcg cctgcccggt
//
