LOCUS       NZ_AP024135            10528 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces sp. EAS-AB2608 strain NBRC 114648 chromosome.
ACCESSION   NZ_AP024135
VERSION     NZ_AP024135.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. EAS-AB2608
  ORGANISM  Streptomyces sp. EAS-AB2608
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Okubo,S., Ena,E., Okuda,A., Kozone,I., Hashimoto,J., Nishitsuji,Y.,
            Fujie,M., Satoh,N., Ikeda,H. and Shin-Ya,K.
  TITLE     Identification of functional cytochrome P450 and ferredoxin from
            Streptomyces sp. EAS-AB2608 by transcriptional analysis and their
            heterologous expression
  JOURNAL   Appl Microbiol Biotechnol 105 (10), 4177-4187 (2021)
   PUBMED   33944982
  REMARK    DOI:10.1007/s00253-021-11304-z
REFERENCE   2  (bases 1 to 9320785)
  AUTHORS   Okubo,S., Ena,E., Okuda,A., Kozone,I., Hashimoto,J., Nishitsuji,Y.,
            Fujie,M., Satoh,N., Ikeda,H. and Shinya,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Contact:Shinya Okubo Eisai Co., Ltd.;
            Tokodai 5-1-3, Tsukuba 300-2635, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Coverage                   :: 53x
            Sequencing Technology             :: Miseq; PacBio RS II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703895.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 09:16:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,174
            CDSs (total)                      :: 8,084
            Genes (coding)                    :: 7,890
            CDSs (with protein)               :: 7,890
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 87 of 194
            Pseudo Genes (incomplete)         :: 132 of 194
            Pseudo Genes (internal stop)      :: 10 of 194
            Pseudo Genes (multiple problems)  :: 32 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:41:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2582310
            Orig. end                         :: 2592838
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024135.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10528
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="13"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10528
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10528
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5528](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5528)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(184..1791)
                     /locus_tag="K6K39_RS11325"
                     /old_locus_tag="EASAB2608_02337"
     CDS             complement(184..1791)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435092.1"
                     /locus_tag="K6K39_RS11325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_02337"
                     /product="dynamin family protein"
                     /protein_id="WP_059249619.1"
                     /transl_table=11
                     /translation="MVTLDVRPQLLDTLSALRDRVAAARFPLPLAGAPRARANRDELLA
                     QLDDYLVPRLREPEAPLLAVVGGSTGAGKSTLVNSLVGRRVSEAGVLRPTTRTPVLVCH
                     PEDHHWFSGMRVLPQLTRVWVPHQDPADEFMLADEDPARVVRVETADTLPRGLALLDAP
                     DIDSLLADNRVLAAELICAADIWIMVTTAARYADAVPWHLLRTAKEYDATLVTVLDRVP
                     HQVVSEVSRQYGALLTKAGLGDVPRFTVPELPESAWGAGLLPATAVAPLKSWLVHHAQD
                     PAARQQVMARTAHGVLDSLRSRMPELASAAAAQYAAALRLTSAVEAAYDKEHARVRARL
                     QAGAVLAGDALKRWRAFPLDCTAGELLDALVESLAALLLCAVTAADERVDDAWRREPAA
                     EAPGLAGRASSLESAEHRIGLAARRWRRELEEYAEDEVRVLERTGAPDPEVVAALVATA
                     VLGGRRARTAGEGLAERLGAHGVLRLRDRAGRLLVEHVDRVMHAERERRLAPLDALDVH
                     PEPQAELIAALSVLQKER"
     misc_feature    1791..1809
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.4"
                     /tool="antismash"
     misc_feature    complement(1802..1818)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.58"
                     /tool="antismash"
     misc_feature    complement(1807..1821)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.96"
                     /tool="antismash"
     tRNA            2113..2188
                     /anticodon=(pos:2584456..2584458,aa:Arg,seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="K6K39_RS11330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Arg"
     gene            2113..2188
                     /locus_tag="K6K39_RS11330"
     gene            2439..3398
                     /locus_tag="K6K39_RS11335"
                     /old_locus_tag="EASAB2608_02338"
     CDS             2439..3398
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672797.1"
                     /locus_tag="K6K39_RS11335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_02338"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_094373940.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 1.5e-54, bitscore:
                     182.9, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQGVATESADWSVIAEQVEVVALREHLDGEEALAA
                     ALADYDIVVTLRERVPFPGSLIARLPRLKLLIASGMRNSVIDYAAAQAHGVTVCGTASS
                     STPPVELTWALLLGLARGIVQESNALRGNGPWQSTVGADLHGRRLGLLGLGKIGSRVAR
                     VGLAFGMRVSAWSRNLTEERAQEVGVERAGSMQELLSGSDFVSVHLALGDRTRGLVGAA
                     ELALMKPTAYLVNTSRAAIVDQDALLAALHEGRIAGAGVDVFDIEPLPADHPMRTAPRL
                     LATPHLGYVSHANYATYYGQAVENIQAYLAGAPVRRLP"
     misc_feature    complement(3612..3625)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.18"
                     /tool="antismash"
     gene            3725..4069
                     /locus_tag="K6K39_RS11340"
                     /old_locus_tag="EASAB2608_02339"
     CDS             3725..4069
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818707.1"
                     /locus_tag="K6K39_RS11340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_02339"
                     /product="chaplin"
                     /protein_id="WP_059249621.1"
                     /transl_table=11
                     /translation="MSRIAKAAAVAVGTGAVVVSGAGLALADAGAQSAAVGSPGVVSGN
                     VIQVPIHIPVNVCGNTVDIIALLNPAFGNACVNDGGKEAPGKPGNPGPGHNGNGGHGNG
                     GYGKDGGYGN"
     gene            complement(4151..5017)
                     /locus_tag="K6K39_RS11345"
                     /old_locus_tag="EASAB2608_02340"
     CDS             complement(4151..5017)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="K6K39_RS11345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_02340"
                     /product="tyrosinase family protein"
                     /protein_id="WP_059249622.1"
                     /transl_table=11
                     /translation="MAYVRKNVRALTAGERRRFVNALLTLKRRGEYDEFVRMHIDFYAA
                     DGEKGLRTAHMAPSFLPWHRRFLLELERALRRVDSSVTVPYWDWTRDRTTTSVPWTKDL
                     LGGNGRRSDRQVMTGPFAYATGQWTIKEGVTDGRFLTRDLGRSAAPLRLPTRSELQWAL
                     DDPVYDAWPWDSTVRKGFRNKLEGWGAGSGSASWKNHNRVHRWVGGAMLGGASVNDPVF
                     WLHHAFLDLQWYRWQRAHRRHRYLPAEPPRASDAQHRRVVARHQKLPPWDETPDQLDDV
                     SRIYRYA"
     gene            complement(5001..5528)
                     /locus_tag="K6K39_RS11350"
                     /old_locus_tag="EASAB2608_02341"
     CDS             complement(5001..5528)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672801.1"
                     /locus_tag="K6K39_RS11350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_02341"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_094373939.1"
                     /sec_met_domain="melC (E-value: 8.3e-45, bitscore: 151.3,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVGVGGVAVGAEQQAAAGAARPSRREVARGLLVSAAALALAPVV
                     AASRPVHQAQADGNSFDETYRGRRIRGVLVPVAGRRAADGEWRITIDGRPLHLMRRADG
                     SWLSMVDHYTSYRTPLEATRAAVDEIGPRQRLRDLAPDRLGGGHADGHSGGHAGGHPGG
                     DADMGGRHGVRA"
     gene            complement(5733..6248)
                     /locus_tag="K6K39_RS11355"
                     /old_locus_tag="EASAB2608_02342"
     CDS             complement(5733..6248)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="K6K39_RS11355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_02342"
                     /product="hypothetical protein"
                     /protein_id="WP_059249624.1"
                     /transl_table=11
                     /translation="MKPTTRGTLAAVVTGVAATVGAAGPAAAAGTVPVPVPLGGVSHAL
                     DMELPRLGAELPLLKPGAPEGPRYVTGRLLPDRALPQLPVSSGLPGLDARAPLPHVLGD
                     GGFDHLDVDAPAGDLRALAPGLSVDAPLTAPDGDNFGLPSAKLPEAGVVAPVLQTVAGG
                     NLATGPGL"
     misc_feature    complement(6344..6364)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 25.53"
                     /tool="antismash"
     gene            complement(6350..6934)
                     /locus_tag="K6K39_RS11360"
                     /old_locus_tag="EASAB2608_02343"
     CDS             complement(6350..6934)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005479492.1"
                     /locus_tag="K6K39_RS11360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_02343"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_059249625.1"
                     /transl_table=11
                     /translation="MRRYAGGSRGLALLIVLTGAAGLLASWVITLDKFKLLEDPGFVPG
                     CSLNPVLSCGSVMESDQASAFGFPNPMLGLAAYGIVICVGMSLLAGARFPGWYWLLFEA
                     GCLFGVGFVSWLQFESLYRINALCLWCCLAWLATIPLFWYVTSVCVREGFLPAPGPVKT
                     FFAEFTWVLPLLHIGVVGMLVLTRWWDFWTS"
     gene            complement(6934..7440)
                     /locus_tag="K6K39_RS11365"
                     /old_locus_tag="EASAB2608_02344"
     CDS             complement(6934..7440)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116484.1"
                     /locus_tag="K6K39_RS11365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_02344"
                     /product="DUF5949 family protein"
                     /protein_id="WP_221334348.1"
                     /transl_table=11
                     /translation="MTSASSEIRTFRAADLGTLALIPWLARNEEGDGRTPCLLACSLGD
                     GPDGPEAASAAVTHLLEDMGLTADSEPVNGTTRPSLKVSMLVVAGQIVLQLPHNISSQV
                     TPPADWLAAVEELGYAYFMLTTRICPSTGPRRPAEGAELAAFAGSPDTTNAAAHVYLPA
                     RGLRA"
     gene            complement(7597..7884)
                     /locus_tag="K6K39_RS11370"
                     /old_locus_tag="EASAB2608_02345"
     CDS             complement(7597..7884)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672804.1"
                     /locus_tag="K6K39_RS11370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_02345"
                     /product="hypothetical protein"
                     /protein_id="WP_059249627.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSVALAGVAAPAFAYDAQHLPPLSLNGGLAHLTE
                     KPPTVQDLLPVQHQSDALDTENKNSVLSSVKGATTTLNQQGLLGGLPLRH"
     gene            complement(8018..9394)
                     /locus_tag="K6K39_RS40920"
     CDS             complement(8018..9394)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K6K39_RS40920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_260335125.1"
                     /transl_table=11
                     /translation="MRDLPARRLASTVLCAAVLVGITGPAAVAAVREHDRAASRAPVPA
                     AEKERLLAQVRALADTDSALHPVIDLLNRSLEGRLPADEARGLGEAAKAAVAKAATAPT
                     APLRPAVPARPASLTAPTTPAAPTSAATPTAPATAAKPAAPTTAATPTAPAKPTATASP
                     VTSAKPASPAAAATPTAPAASSTSAKPASPAASATPTAPASPAASATPTAPASPAASAK
                     PAAPSAPASASASASASAKPASPAASATPTAPAPASASAKPASPAASATPTAPASPASS
                     AKPASSAKPAAPSAPATTPAASTAPTTPASSAGPASSASPSASAATPAAPAAPAASAAP
                     MAFAVPAALTAQTAMRQGDVLGVPSSRDLTSDALSALQTAIDNLVNAVTEQLDNLLSSA
                     TDVVSGLVDLLGTTLLGKDTAAPSVDTLPALPSLPAEPTQSAQATQPAQTAQPTQSTTS
                     "
     gene            complement(9565..9804)
                     /locus_tag="K6K39_RS11380"
                     /old_locus_tag="EASAB2608_02348"
     CDS             complement(9565..9804)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="K6K39_RS11380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_02348"
                     /product="chaplin"
                     /protein_id="WP_059249630.1"
                     /transl_table=11
                     /translation="MSRIAKGLVLTSAAVAAVAGGAGVASADAGSGAVAAHSPGVLSGN
                     AIQVPIHVPINLCGNTIDIVGLLNPAFGNTCVND"
ORIGIN
        1 gctcaggcgc gcctccggga acccgccggt ccgctcaaga ccgtcccggg caccggaacc
       61 ggggtgaccg ctgtgctccc tgcccccgct gcccccgcgg tcgccgctgt cgctgctgtg
      121 gtcgatgcgc ctcatgtgct cggtgtgatc cgtgtggtcc tggtcagtga cggcggtcac
      181 cggtcacctc tccttctgca gtacggacag cgcggcgatg agttcggcct ggggctcggg
      241 gtggacgtcc agggcgtcga gcggggcgag gcgccgctcg cgttcggcgt gcatgacgcg
      301 gtccacgtgc tcgacgagca gccgaccggc ccggtcgcgc agccgcagca cgccgtgcgc
      361 gccgagccgc tcggcgagcc cctcgcccgc ggtacgggcc cggcgcccgc ccagtacggc
      421 cgtggcgacg agggcggcga cgacctcggg gtcgggcgcg ccggtgcgct ccaggacccg
      481 tacctcgtcc tcggcgtact cctccagctc gcgccgccag cgccgggccg cgagcccgat
      541 gcggtgctcg gcgctctcca gggaggacgc gcggccggcg aggccggggg cctcggcggc
      601 gggctcgcgc cgccaggcgt cgtcgacgcg ctcgtccgcg gcggtgacgg cgcacagcag
      661 gagggcggcc aggctctcca cgagggcgtc caggagttct cccgcggtgc agtccagggg
      721 gaaggcgcgc cagcgcttga gggcgtcccc ggcgagtacg gcgccggcct ggagccgggc
      781 ccgcacgcgc gcgtgctcct tgtcgtaggc ggcctccacg gccgaggtga gccggagggc
      841 cgccgcgtac tgggcggcgg ccgcgctggc cagctcgggc atgcgggacc tgagggagtc
      901 gaggacgccg tgcgccgtac gggccatgac ctgctgccgg gcggcggggt cctgggcgtg
      961 gtgcacgagc cagctcttca ggggcgcgac ggcggtggcg ggcagcaggc cggcgcccca
     1021 ggcggattcg ggcagctcgg gcacggtgaa ccggggtacg tcaccgaggc cggccttggt
     1081 gagcagggcg ccgtactgcc gggagacctc ggagacgacc tggtggggga cccggtccag
     1141 gacggtgacg agggtcgcgt cgtactcctt ggcggtgcgc agcaggtgcc aggggacggc
     1201 gtcggcgtag cgggcggccg tggtgaccat gatccagatg tcggcggcgc agatcagttc
     1261 ggcggccagg acgcggttgt cggcgagcag ggagtcgatg tcgggtgcgt cgaggagggc
     1321 gagcccgcgc gggagggtgt cggcggtctc gacgcgcact acgcgcgcgg ggtcctcgtc
     1381 ggcgagcatg aactcgtcgg cggggtcttg gtggggcacc cacacgcggg tgagctgagg
     1441 gagcacgcgc atgccgctga accagtgatg atcctccgga tggcagacaa ggaccggtgt
     1501 ccgggtggtc ggccgcagca cgccggcctc actcacccgc cggccgacga gggagttcac
     1561 gagcgtcgac ttgcccgcgc cggtggagcc gccgacgacg gccagcagcg gcgcctcggg
     1621 ctcccgtagg cggggtacca ggtagtcgtc gagctgcgcg aggagttcgt cgcggttcgc
     1681 acgcgcgcgt ggcgcccccg cgaggggcag cggaaagcgt gcggcggcga cacggtcgcg
     1741 cagagcggaa agtgtgtcga gcagctgagg ccgtacgtcc aaggtcacca catgcgaaga
     1801 atgcccaatt ttaggggaat tctgaagcat atagacatgt ctgcgcgccg acgggacaga
     1861 acggacggaa gggatgaccg ggacatggcc gcagtccagg cataacgagt gcacaacacc
     1921 cggtgccgtc gggcaccaaa agcggtgcac gattcgtacc tgcctgcgat tatcaggacc
     1981 gcttcaccga acctccacat cgagccacgg aggggaagcg acagggtcaa ggaaccggga
     2041 gccctatcct tgaccccggc aacgtcacgg atcagcccac cccagggcac cgcacacgag
     2101 gccaccccac cggcccccgt agctcagtgg atagagcagg cgccttctaa gcgcttggcc
     2161 gcaggttcga gtcctgccgg gggcgccagg ctccgccctc cccgccggga gggcgttcgt
     2221 gctgttcagg ccgcgtcgcc tcgaccgccg ggcccgccct ggggaacctc gggcgctgcc
     2281 cccgcgaccg atcgtcccga gggccgtcgt ccctgatggg cgtccggtgc gcagtcgtac
     2341 ctgatgggcg tccggtgttc ggaccgcagg gggaggcccg cctcggcggg agaagcgcgg
     2401 cgacgggtcg gtacagtcac ccctggaggg gagacgacgt gcgacttcgc tgtgcggtgc
     2461 tggacgactt tcagggtgtg gcgaccgagt cggccgactg gtcggtgatc gcggagcagg
     2521 tggaggtcgt cgcgctgcgc gagcatctcg acggggagga ggccctcgcc gcggcgctcg
     2581 ccgactacga catcgtcgtc accctgcgcg aacgcgtccc cttccccggc tccctgatcg
     2641 cccgcctccc ccggctgaag ctgctcatcg cctccggcat gcgcaacagc gtcatcgact
     2701 acgccgccgc gcaggcgcac ggcgtcaccg tgtgcggtac ggcgagttcg agcacccctc
     2761 cggtcgagct gacctgggca ctgctgctcg gcctggcccg cggcatcgtc caggagagca
     2821 acgcgctgcg cggcaacggc ccctggcagt cgaccgtcgg cgccgacctg cacggccgtc
     2881 ggctgggcct gctcgggctg gggaagatcg gcagccgggt ggcccgggtc gggctcgcct
     2941 tcgggatgcg ggtcagcgcg tggagccgga atctcaccga ggagcgcgcc caggaggtgg
     3001 gcgtcgaacg tgccggctcg atgcaggagt tgctgagcgg cagcgacttc gtctccgtcc
     3061 acctcgccct cggcgaccgc acccgcggcc tcgtcggtgc cgccgaactc gccctcatga
     3121 aaccgaccgc ctacctcgtc aacacctccc gcgccgccat cgtcgaccag gacgcgctgc
     3181 tggccgcgct gcacgagggc cggatcgccg gcgccggcgt cgacgtcttc gacatcgagc
     3241 cgctgcccgc cgaccatccg atgcgcaccg cgccccgcct cctcgccaca ccccacctcg
     3301 gctacgtctc ccacgccaac tacgcgacgt actacggcca ggccgtggag aacatccagg
     3361 cctacctggc gggcgctccg gtacgacggc tcccctgaga ccactgagtc ctctgagact
     3421 cctcaggcgc tggaggctcc ctgcgggccc cctgcgggcc ggtcgggctt cgcacctctc
     3481 cgccgccctt ccgcgcgcgg cctgcgatcc acccctggcc cgggcccgcc gctccccgcc
     3541 gctccccgcc gtttctgccg tccgccattc gtgctgcccc cttatgcctg ctgtccctgc
     3601 tgtccctgct gttaaaagag tgatccccgg ctgatcaccg tgacccgccc cgcgtcgcgc
     3661 cgttgaacgg aaagtgcggc ggctgctctg ccgctgcgct ttccagctac aaaaggagaa
     3721 cgtgatgtct cgcatcgcga aggcagccgc tgtcgctgtc ggcaccggtg ccgtggtggt
     3781 cagcggggcc gggctggccc tggccgacgc gggtgcccag tccgcggccg tcggatcgcc
     3841 cggcgtggtg tccggcaatg tcattcaggt tccgatccac atcccggtca acgtgtgcgg
     3901 caacaccgtc gacatcatcg ccctgctgaa cccggcgttc ggcaacgcct gcgtcaacga
     3961 cggcggcaag gaggcgcccg gcaagcccgg caaccccggc ccgggtcaca acggcaacgg
     4021 cggccacggc aacggcggct acggcaagga cggcggctac ggcaactgag ccgaccggca
     4081 ccccaggcaa gagccccggc atccgagcgc cggggctctt cccgtgtccg gccgcgcgtc
     4141 cggcaccccc tcacgcgtac cggtagatcc tgctgacgtc gtcgagctgg tccggcgtct
     4201 cgtcccacgg cggcagtttc tggtgccggg cgacgacccg cctgtgctgg gcgtcggagg
     4261 cgcggggcgg ctcggccggc aggtagcggt gcctgcggtg ggcccgctgc cagcggtacc
     4321 actgcaggtc gaggaaggcg tggtgcagcc agaacaccgg atcgttgacg gacgcgccgc
     4381 cgagcatcgc accgccgacc cagcggtgca cccggttgtg gttcttccag gaggcgctgc
     4441 cgctgccggc gccccagccc tccagcttgt tgcggaagcc cttgcggacg gtcgagtccc
     4501 agggccacgc gtcgtagacg ggatcgtcca gggcccactg cagctcgctc cgggtgggca
     4561 gccggagggg cgcggcggag cggccgaggt cccgggtgag gaacctgccg tcggtgacgc
     4621 cctccttgat ggtccactgg cccgtcgcat aggcgaacgg cccggtcatc acctgccggt
     4681 cggagcgccg cccgttcccg ccgagcaggt ccttcgtcca cggcacggag gtcgtcgtac
     4741 ggtcccgggt ccagtcccag tacggcaccg tcaccgagga gtcgacccgg cgcagcgccc
     4801 gttccagttc cagcaggaac cggcggtgcc aggggaggaa ggagggcgcc atgtgggcgg
     4861 tgcgcaggcc cttctcgccg tcggcggcgt agaagtcgat gtgcatgcgc acgaactcgt
     4921 cgtactcacc gcgccgcttg agggtcagca gcgcgttcac gaaccgccgc cgctcgcccg
     4981 cggtgagcgc cctgacgttc ttacgcacgt acgccatgcc gtccccccat gtccgcgtct
     5041 ccccccggat gaccgccggc gtgcccgcct gagtgtccgt cggcgtgtcc accgccgagc
     5101 cggtccggcg ccaggtcgcg cagccgctgg cggggcccga tctcgtccac ggccgcccgg
     5161 gtcgcctcca gcggcgtccg gtacgacgtg tagtggtcga ccatgctcag ccagctgccg
     5221 tcggcgcggc gcatcaggtg cagcgggcgg ccgtcgatgg tgatccgcca ctcgccgtcg
     5281 gccgccctgc gccccgcgac gggcaccagc acgccccgga tgcgacggcc ccggtaggtc
     5341 tcgtcgaagg agttgccgtc ggcctgggct tggtgcacgg gccgggaggc ggcgacgacc
     5401 ggggccagcg ccagcgcggc ggccgagacc agcagcccgc gcgccacctc cctccgggac
     5461 ggtctggccg cccccgctgc ggcctgctgc tccgcaccga ccgccactcc gccgacaccg
     5521 acgaccatgc tcgctcctcg tccgggttca gatgtccgta ccggctaacc ggccgagggc
     5581 gcgcggggtc accacggacg agccatacga gcgagacgga cgagaagaac gagaagaacg
     5641 agcgagcacg acaagccgta cgagccgtac gagccgtacg agccgtgagc cgtgtgagcc
     5701 gggcgtcgta cggcgggtgc ggccgggccg ggttacagtc ccgggcccgt ggccaggttg
     5761 ccgccggcga cggtctgcag gaccggggcg acgacgccgg cctcggggag cttggcggac
     5821 ggcaggccga agttgtcgcc gtcgggcgcg gtcagcgggg cgtccacgga caggccgggg
     5881 gcgagcgcgc gcaggtcgcc ggcgggcgcg tccacgtcca ggtggtcgaa gccgccgtcg
     5941 ccgaggacgt gcggcagcgg agccctggcg tccaggccgg gcaggccgct gctcaccggc
     6001 agctggggca gggcccggtc ggggaggagc cggcccgtga cgtaccgggg cccctcgggg
     6061 gcaccgggct tgagcagcgg cagctcggcg ccgagccggg gcagctccat gtccagcgcg
     6121 tgggagacgc cgcccagggg tacggggacg gggaccgtgc cggcggccgc ggcgggaccg
     6181 gcggccccca cggtggccgc gacgcccgtg acgacggcgg caagggttcc tcgggtggtc
     6241 ggcttcatgt caggtacggt ccctttcagg atgcgggggg ttcgacgtcc gtaccgcgta
     6301 acgagcggaa atcccctgca tttcacaatt cccccgagtg ccgtaatagt cagctcgtcc
     6361 agaagtccca ccagcgggtc agtacgagca ttccgacgac accgatgtgc agcagcggaa
     6421 gcacccaggt gaattcggcg aagaatgtct tcaccgggcc gggcgcgggc aggaagccct
     6481 cgcgtacgca gaccgacgtc acgtaccaga agaggggaat ggtcgcgagc caggccaggc
     6541 agcaccacag gcacagcgcg ttgatccggt acagcgactc gaactgcaac caggacacga
     6601 acccgacgcc gaacaggcag ccggcctcga acagcagcca gtaccagccg gggaagcggg
     6661 cgccggccag caggctcatg ccgacgcaga tcacgatgcc gtaggccgcc aggcccagca
     6721 tcgggttggg gaacccgaag gccgaggcct ggtcgctctc catcacgctg ccgcaggaca
     6781 gcaccgggtt caggctgcac ccggggacga agccgggatc ctccagcagc ttgaacttgt
     6841 ccagggtgat gacccaggag gcgagcagtc cggcggcccc cgtgagcacg atcagcaggg
     6901 cgaggccgcg gctgccgccc gcgtaccggc gcatcaggcc cgcaggccgc gggcaggcag
     6961 gtagacgtga gcggcggcgt tcgtggtgtc cggggagccc gcgaacgcgg ccagctccgc
     7021 gccctcggcc ggccggcggg ggccggtgga ggggcagatg cgggtggtga gcatgaagta
     7081 ggcgtagccg agttcctcga ccgcggccag ccagtccgcc ggcggggtga cctggctgct
     7141 gatgttgtgg ggcagctgga ggacgatctg gccggcgacg accagcatgc tgactttcag
     7201 gctcggccgg gtggtgccgt tcacgggctc gctgtccgcg gtcagtccca tgtcctccag
     7261 gaggtgcgtg acggcggcgg acgcggcctc cgggccgtcc gggccgtcgc ccagggagca
     7321 ggcgagcaga cagggggtcc ggccgtcgcc ctcctcgttc ctggccagcc aggggatcag
     7381 ggcgagcgtg ccgaggtcgg cggcgcggaa ggtgcggatt tcgctggatg ctgaggtcac
     7441 cgcgcgacca tatcgacggg ccgccgcccg gtccggcggc ttctcacccg tccggagcag
     7501 cgcccgggac ggcgccacac gatcgagcga cggggtcccg gacacgccga gggccggttc
     7561 cgcacggggt gcggtaccgg cccgggcggg ggcgcgtcag tgccgcaggg gcaggccgcc
     7621 gagcagcccc tgctggttga gggtggtggt ggcgcccttg acgctgctca gcacggagtt
     7681 cttgttctcg gtgtcgagcg cgtccgactg gtgctgcacc ggcagcaggt cctgcacggt
     7741 gggcggcttc tcggtgaggt gggcgaggcc gccgttgagg ctcagcggcg gcaggtgctg
     7801 ggcgtcgtag gcgaacgcgg gtgcggcgac gccggcgagg gccacggaac cagcgacaac
     7861 ggcggcagcc ttgagggact tcatcgtgtt cctttcttcg gcaactcacg tcaccccggg
     7921 atttccgacg ccgtgcctaa cgagttccgg acggaccgga aacccggcac cgagaagaca
     7981 tatgagatcc ccgtgcgccc accgggaatc gcccgcgtca gctggtcgtc gactgggtgg
     8041 gctgagccgt ctgggccggc tgagtcgcct gtgccgactg ggtgggctcc gcggggaggc
     8101 tgggcagcgc gggcagggtg tcgacgctgg gagcggccgt gtccttgccg aggagggtcg
     8161 tacccagcag gtcgaccagg cccgacacca cgtcggtggc ggacgacagg aggttgtcga
     8221 gctgttcggt gaccgcgttc accagattgt cgatcgcggt ctgcagggcg gacagcgcgt
     8281 cactggtcag gtcgcgggag gaggggacgc cgaggacgtc gccctgccgc atggccgtct
     8341 gcgcggtgag ggccgccggt acggcgaagg ccatgggggc ggccgacgcg gcgggagcgg
     8401 ccggcgcggc aggggtggcg gccgacgccg aggggctggc cgaggaagcg gggccggccg
     8461 acgacgccgg ggtggtgggc gccgtggagg ccgcgggagt ggtggccggg gcgctcggtg
     8521 cggcgggctt ggcggaggac gcgggcttgg cggaggacgc gggggacgcg ggcgccgtcg
     8581 gggtggcgga agccgcgggg gaggcgggct tcgccgacgc agacgcgggt gcgggcgcgg
     8641 tgggggtggc cgaggccgcg ggcgaggccg gcttcgccga cgccgacgca gatgcggacg
     8701 cggacgcggg cgccgaagga gccgccggct tggcggaggc cgcgggagac gcgggggcgg
     8761 tcggtgtggc ggaggccgcg ggagacgcgg gggcggtcgg cgtggcggag gccgcgggag
     8821 acgccggctt ggccgacgtg gacgaagcgg ccggcgcggt cggggtcgcg gctgccgcgg
     8881 gtgaggcggg cttcgcggac gtcaccgggg aggcggtcgc cgtcggcttg gcgggtgccg
     8941 tgggcgtcgc ggccgtggtg ggcgccgcag gcttcgccgc cgtcgccggt gccgtgggcg
     9001 tcgccgccga cgtaggtgcc gcaggcgtcg tgggtgccgt gagggacgcg ggtcgtgccg
     9061 gtaccgccgg ccggagaggg gccgtcgggg cggtggcggc cttggcgacg gccgccttgg
     9121 ccgcctcgcc cagtcctctg gcctcgtcgg cggggagccg gccctccagc gaccggttga
     9181 gcaggtcgat gaccggatgg agcgcggagt cggtgtcggc cagcgctctg acctgggcga
     9241 gcagcctctc cttctccgct gccgggacag gcgcccggct ggcggcgcgg tcgtgctccc
     9301 ggaccgcggc gacggcggcc gggccggtga tgccgaccag gacggccgcg cagaggacgg
     9361 tggacgcgag acgccgggca ggcagatcac gcatgggtgt tcctttcggt gaacagtggg
     9421 acctgagccc accgtcagtt caccgatcgc cctccgcaac cgctcgccca caccaagtga
     9481 cccgtccggg gagcagaacc ggccgtccgc cccgcgacgg atgcacgcgg ggcggacggc
     9541 cggccgaagg gtcgcgaccc gacgtcagtc gttgacgcag gtgttgccga acgccgggtt
     9601 cagcaggccg acgatgtcaa tggtgttgcc gcacaggttg atcgggacgt ggatcgggac
     9661 ctggatggcg ttgcccgaca gcacgcccgg cgagtgggcg gcgacggcgc ccgaaccggc
     9721 gtcggcggaa gccacgccgg caccgccggc gacggccgcg acggcggcgg aggtcaggac
     9781 caggcccttc gcgatacgcg acatggagag gtgctccttg gagatcgaca caaacatccg
     9841 ggcgggatgc ccggcgttgt gtcaacgcgc ggcactcccg ggagttgtgc cgccaatgga
     9901 gtgatccacg gacggaacgc cttcacactt ccgacacact tgccctgttt ctcctgattg
     9961 gtgcggctgc cgactagagt tgcggaccct ccggccgccg cccgagtcgg cgcgggcgac
    10021 ccgcgcacac tctcctccgc cctccggcgt acgcccgtcg caccgagcgg ccacaccggt
    10081 ccaccgaacg acgtagcgaa ctccacgctc agcgaaccgg gcgacgtacc ggccgggcgg
    10141 gttccggcct acggccgcac gctctgtcac ggctccgagg tgtccgatct cggtccggct
    10201 ccgcacggcg gcggtgacca gccgggctca ctgctcgttt caccacgcgg acgcggcgtg
    10261 ggccggcgca cgcttcgtcg ccgtaacacg cagaactgac aggcgcgttc acgaccgcag
    10321 tcccgagcgc cccgagcgag gtaccccgcg catgcaccag tcagcacccg cccatcggcc
    10381 ccgggtcctg cacctcgcac aacccgtcga cggcggtgtc gcccgggtcg tgctcgacct
    10441 ggcccgggcg cagctcgcgg cgggcctgcg ggtcacggcg gcctgccccg gcgggccgct
    10501 ggccgccggc ctgcgggagc tgggcgcc
//
