LOCUS       NZ_AP024135            10405 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces sp. EAS-AB2608 strain NBRC 114648 chromosome.
ACCESSION   NZ_AP024135
VERSION     NZ_AP024135.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. EAS-AB2608
  ORGANISM  Streptomyces sp. EAS-AB2608
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Okubo,S., Ena,E., Okuda,A., Kozone,I., Hashimoto,J., Nishitsuji,Y.,
            Fujie,M., Satoh,N., Ikeda,H. and Shin-Ya,K.
  TITLE     Identification of functional cytochrome P450 and ferredoxin from
            Streptomyces sp. EAS-AB2608 by transcriptional analysis and their
            heterologous expression
  JOURNAL   Appl Microbiol Biotechnol 105 (10), 4177-4187 (2021)
   PUBMED   33944982
  REMARK    DOI:10.1007/s00253-021-11304-z
REFERENCE   2  (bases 1 to 9320785)
  AUTHORS   Okubo,S., Ena,E., Okuda,A., Kozone,I., Hashimoto,J., Nishitsuji,Y.,
            Fujie,M., Satoh,N., Ikeda,H. and Shinya,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Contact:Shinya Okubo Eisai Co., Ltd.;
            Tokodai 5-1-3, Tsukuba 300-2635, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Coverage                   :: 53x
            Sequencing Technology             :: Miseq; PacBio RS II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703895.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 09:16:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,174
            CDSs (total)                      :: 8,084
            Genes (coding)                    :: 7,890
            CDSs (with protein)               :: 7,890
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 87 of 194
            Pseudo Genes (incomplete)         :: 132 of 194
            Pseudo Genes (internal stop)      :: 10 of 194
            Pseudo Genes (multiple problems)  :: 32 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:41:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1499989
            Orig. end                         :: 1510394
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024135.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="10"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    267..281
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.71"
                     /tool="antismash"
     misc_feature    complement(381..395)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.0"
                     /tool="antismash"
     gene            complement(750..1601)
                     /locus_tag="K6K39_RS06470"
                     /old_locus_tag="EASAB2608_01355"
     CDS             complement(750..1601)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121392.1"
                     /locus_tag="K6K39_RS06470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_01355"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_059248612.1"
                     /transl_table=11
                     /translation="MKTLVGRRTRLLAATTATAALVLVAAGCSSDDNGGSGKTTVKGVH
                     LAKAGQLTTCTHLPYPPFQSEVDGKVQGFDVSLIDLVAEDLGVKQQIRDTPFENFKTGA
                     FLNSGECDLAAAGMTITDERKKNVDFSDPYFEATQAVLVDKDSGIGSLADVKAKGKKLG
                     AQAQTTGEDYAKTKGYDPVSFESSDAVLNGLRTGQVQAVVIDYPVVQGWLKDKATAAKF
                     KVVDNLKTGEQYGFTVKKGNKALLDAINKALKTAKADGTYKKIYEQWIGPYDASVASPA
                     AS"
     misc_feature    complement(1594..1609)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 18.27"
                     /tool="antismash"
     gene            1752..2852
                     /locus_tag="K6K39_RS06475"
                     /old_locus_tag="EASAB2608_01356"
     CDS             1752..2852
                     /NRPS_PKS="Domain: Aminotran_5 (74-294). E-value: 8.7e-28.
                     Score: 89.2. Matches aSDomain:
                     nrpspksdomains_K6K39_RS06475_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="K6K39_RS06475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_01356"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_086696565.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.8e-21, bitscore:
                     74.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLSAERFAAIEDGVARLLDTRQDVLITQGEALLPLE
                     GAIRAAAGPGTVALNVITGPYGQTFGNWLRDAGATVHDLSVPFHTAATAEQIRQAFAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEAVRAQGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAISVSERAWTRMAANPNAPRRSYLSLLDWKERWIDAGRTALPHAP
                     AQLEMLALEACLERIESAGLQTVMARHRRAALATRAGAVALGGGLEPYVYEERDAAPVA
                     TTLRVPSGVAASDLVARALATDPTLPLAAGGGALAKEMIRVNHYGPDATPGAVQNSLAA
                     LGAALAEQGLPVDPAAARRAAELAWG"
     aSDomain        1974..2633
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K6K39_RS06475_Aminotran_5.1"
                     /evalue="8.70E-28"
                     /label="K6K39_RS06475_Aminotran_5.1"
                     /locus_tag="K6K39_RS06475"
                     /protein_end="294"
                     /protein_start="74"
                     /score="89.2"
                     /tool="antismash"
                     /translation="RDAGATVHDLSVPFHTAATAEQIRQAFAEHPEIDFVSLVHAEAAT
                     GNTNPVAEIGEAVRAQGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSA
                     ISVSERAWTRMAANPNAPRRSYLSLLDWKERWIDAGRTALPHAPAQLEMLALEACLERI
                     ESAGLQTVMARHRRAALATRAGAVALGGGLEPYVYEERDAAPVATTLRVPSGVAASD"
     misc_feature    2913..2928
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.1"
                     /tool="antismash"
     misc_feature    2916..2929
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.21"
                     /tool="antismash"
     misc_feature    3035..3050
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            3169..3687
                     /gene="ectA"
                     /locus_tag="K6K39_RS06480"
                     /old_locus_tag="EASAB2608_01357"
     CDS             3169..3687
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671932.1"
                     /locus_tag="K6K39_RS06480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_01357"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_059248614.1"
                     /transl_table=11
                     /translation="MTAAPADLLIERPATTDGAALWRLAKESKTLDLNSSYSYVLWCRD
                     FAGTSAVARGAGGEPVGFVTGYLRPDRPRTLLVWQVAVDPAHRGHGIAAKLLDGLTARL
                     TAERGITELETTITPGNTASERLFTSFAERHGAGVSREVLFPADLFPDGPHDPEVLYRI
                     GPLTEFTAH"
     gene            3701..4979
                     /gene="ectB"
                     /locus_tag="K6K39_RS06485"
                     /old_locus_tag="EASAB2608_01358"
                     /pseudo=""
     CDS             3701..4979
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="K6K39_RS06485"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_01358"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPVVFDRAQGSRMYDEDGHAY
                     LGRALLRRCRITQLRPQQPGAETGVARLSGTGRGHPRPGHVDHGQAVVPAGVPGAGAAS
                     A"
     gene            5001..5405
                     /locus_tag="K6K39_RS06490"
                     /old_locus_tag="EASAB2608_01359"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="K6K39_RS06490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_01359"
                     /product="ectoine synthase"
                     /protein_id="WP_059248615.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.4e-53, bitscore:
                     178.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETILY
                     AGTETSMWYANHIEAVVCTKGEAELTDHEAGKTYTITPGTMYLLDGHERHTLRVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5412..6305
                     /gene="thpD"
                     /locus_tag="K6K39_RS06495"
                     /old_locus_tag="EASAB2608_01360"
     CDS             5412..6305
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="K6K39_RS06495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_01360"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_059248616.1"
                     /sec_met_domain="PhyH (E-value: 1e-38, bitscore: 132.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTATVTDLYPTRGTTEVTVPRQDPVVWGSPDTPGPITTADLQAY
                     ERDGFLAVDQLITEDEVAVYKRELDRLVSDPAIRADERSIVEPKSQEIRSVFEVHRISE
                     VFANLVRDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENYDTNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTPSDEALT
                     SLASEYGIRLFTGRAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAVEPFAAP
                     VRRPEFIGARDFTPVK"
     gene            complement(6370..7476)
                     /locus_tag="K6K39_RS06500"
                     /old_locus_tag="EASAB2608_01361"
     CDS             complement(6370..7476)
                     /NRPS_PKS="Domain: Aminotran_5 (35-310). E-value: 2.7e-24.
                     Score: 77.7. Matches aSDomain:
                     nrpspksdomains_K6K39_RS06500_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989745.1"
                     /locus_tag="K6K39_RS06500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_01361"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_189397446.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.9e-18, bitscore:
                     64.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGWFPGTQSGPMETTETTETFDRLVRAEFRPRGSYLNTASTGLL
                     PARTVTALQEAALLRAEGRPLLPLYEEVEVCRAAYARLAGVPATRVAAGASVAAHTGVI
                     AASLPAGAEVLTAEDDFTSVLNPFHIRGDLKVRTVPLERIAESVRPGTALVAVSAAQSA
                     DGRIADLPALREAARAHGARTYVDFSQAAGWLPMAADAYDFTATVSFKWLMGPHGAAFL
                     TVPEDFGGLTPVLAGWVAAERPWDSCYGPVAELAHSARRFDLTHALFTFAGLRRSLELI
                     EELGVEAIHAHDLALADRFRAGLADLGHEPVPAPGSAIVSVPGLGSRQPALSAAGVEVS
                     DRAGNLRAAFHLYNTAADVDRLLAALSG"
     aSDomain        complement(6547..7371)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K6K39_RS06500_Aminotran_5.1"
                     /evalue="2.70E-24"
                     /label="K6K39_RS06500_Aminotran_5.1"
                     /locus_tag="K6K39_RS06500"
                     /protein_end="310"
                     /protein_start="35"
                     /score="77.7"
                     /tool="antismash"
                     /translation="YLNTASTGLLPARTVTALQEAALLRAEGRPLLPLYEEVEVCRAAY
                     ARLAGVPATRVAAGASVAAHTGVIAASLPAGAEVLTAEDDFTSVLNPFHIRGDLKVRTV
                     PLERIAESVRPGTALVAVSAAQSADGRIADLPALREAARAHGARTYVDFSQAAGWLPMA
                     ADAYDFTATVSFKWLMGPHGAAFLTVPEDFGGLTPVLAGWVAAERPWDSCYGPVAELAH
                     SARRFDLTHALFTFAGLRRSLELIEELGVEAIHAHDLALADRFRAGLADLGHE"
     gene            complement(7538..8254)
                     /locus_tag="K6K39_RS06505"
                     /old_locus_tag="EASAB2608_01362"
     CDS             complement(7538..8254)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140791.1"
                     /locus_tag="K6K39_RS06505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_01362"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_059248618.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDEVEVVHRSFELDP
                     GRAKGDVQPVITMLTRKYGMSEAQAQAGEDNLGAQAAAEGLDYRTRDRDHGNTFDMHRL
                     LHFAKEHGRQDRLLQLLYRANFAEERSVFTEGDERLVELAVEAGLDAEAARAVLTDPDA
                     YADDVRADEREAARLGATGVPFFVLDRTYGVSGAQPAEVFTQALTQAWGERSPLKLVDQ
                     GGAEACGPDGCAVPQH"
     gene            complement(8330..8836)
                     /locus_tag="K6K39_RS06510"
                     /old_locus_tag="EASAB2608_01363"
     CDS             complement(8330..8836)
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130468.1"
                     /locus_tag="K6K39_RS06510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_01363"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_237481934.1"
                     /transl_table=11
                     /translation="MISHHERGPRTIRGALPAEAENIAALHRRARSTYYPDGLPDDGID
                     WTAAWRGAVERPDGQVLCVVEEGTILGIASFRPPQDGPAGTVKLFQFHVDPDHWRRGIG
                     TALHAACVARWRADGRHCAVLDVHVDNLRARSFYARHGWVPDPAHPPAPGDHHLFLRLS
                     VPPGE"
     gene            complement(8833..9810)
                     /locus_tag="K6K39_RS06515"
                     /old_locus_tag="EASAB2608_01364"
     CDS             complement(8833..9810)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545805.1"
                     /locus_tag="K6K39_RS06515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_01364"
                     /product="GlxA family transcriptional regulator"
                     /protein_id="WP_059254892.1"
                     /transl_table=11
                     /translation="MLRIAVVASPPVSMFNLAIPELLFDKVRVDGRPGYEVVICAPDPG
                     PVPTTGTLELHVPRGLEALGEADTVLVAGTGQPFAPDPRVIEALREAAGRGKRIASICS
                     GAFTLAEAGLLKGRKATTYWAHAEEMRRRHPDIDVRGDVLYAQDGPFLTSSGYAAGIDL
                     CLHIIRTDYGAAVANEVARLALVAPVRPGGQTQFTQTPLPPERGTACADTRGWAMRNLD
                     KPLTLTDLARHAGVSVRTLTRRFHAESGVSPLQWLLHQRIERAKELLETTRLPMDQVAA
                     ACGLGTADSLRAHLVRRTGLTPSAYRAQFSRHATGRPTAASSAA"
     misc_feature    complement(9989..10002)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.13"
                     /tool="antismash"
ORIGIN
        1 gcccgcgacc aggatcggcg tggagttggc ggtcgtactg gcgaggtcgc ggccgaactt
       61 ggacagctcc cgctcctcca gggtgacgcc gaggaacagc accagcgagg agtccttgaa
      121 gagcaggacg agttcgttgg tgagcggcgg caggatgatc cggaaggcct gcgggacgat
      181 gatcgagacc atcgccttcg ccggcgagaa gccgagcgaa cgcgccgctt ccatctgccc
      241 cttgggcacc gcctggatgc ctgcgcggat ggtctccgcc atgtacgcgg ccgagaccag
      301 gccgagcgcg agggcgacct tgccgtaggt gccgcccggg atctccgtgc ccgggaaggc
      361 gagcgggacg gccacgccga cgaagatgaa gatcagcagg gcgggcaggc cgcggaagat
      421 ctcgatgtag acgcccgcca gccagcggta cggccccacc gaggacagcc gcatcagcgc
      481 gatgaccatg ccgaggacga ggccgacgac gaagccggac agcgtgtaca gcacggtgtt
      541 cttcagcgcc agcgtgatga cgtccgggaa catctgccgg gcgaggtcgg cctgggcgaa
      601 ctggttctgc agccggtccc agtcggccgt gaccgcgaag gcgatcacgg cggcgacgaa
      661 gacggcgtac tgcacgcccc gcgacaggct gcgcttctgg cgccgggtca gccccttctt
      721 cttcggctgg agggttgtgt ccgtgtcggt catgaggcgg cgggagaggc gacggaggcg
      781 tcgtacgggc cgatccactg ctcgtagatc ttcttgtagg tgccgtcggc cttggcggtc
      841 ttgagcgcct tgttgatggc gtcgagcagc gccttgttgc ccttcttcac ggtgaagccg
      901 tactgctcac cggtcttgag gttgtcgacc accttgaact tggccgcggt ggccttgtcc
      961 ttcagccagc cctggacgac cgggtagtcg atgacgacgg cctggacctg gccggtgcgc
     1021 aggccgttca ggacggcgtc ggaggactcg aaggagaccg ggtcgtagcc cttggtcttg
     1081 gcgtagtcct cgccggtggt ctgcgcctgg gcgccgagct tcttgccctt ggccttgacg
     1141 tcggcgagcg agccgatccc gctgtccttg tcgaccagga cggcctgggt ggcctcgaag
     1201 tacgggtcgg agaagtcgac gttcttcttg cgctcgtcgg tgatggtcat accggcggcg
     1261 gccaggtcgc actcgccgga gttcaggaag gcgccggtct tgaagttctc gaagggcgtg
     1321 tcgcggatct gctgcttgac gccgaggtcc tcggcgacca ggtcgatcag ggagacgtcg
     1381 aagccctgca ccttgccgtc gacctccgac tggaacggcg ggtagggcag gtgggtgcag
     1441 gtggtcagct gaccggcctt ggccaggtgg acgcccttga ccgtggtctt gccgctcccc
     1501 ccgttgtcgt ccgaggagca gccggccgcg acgagcacga gtgcggcggt cgcggtggtg
     1561 gcggccagaa ggcgggtccg gcgccctacg agcgttttca tggggagatc tcctgtggtg
     1621 gaactgtggc ttccgattat aaggaagggt ttgagtctct caaatcaaac ccatggttac
     1681 gaagcctttc gacctaagat cggtgaagtc gacccgccca gagcgaatcc gagagcgaag
     1741 agagcaccgc cgtgacccac cccttcctcg acctgccccc gctgagtgcc gagcgcttcg
     1801 ccgccatcga ggacggggtg gcccggctgc tggacacccg gcaggacgtc ctgatcaccc
     1861 agggcgaggc gctgctcccg ctggagggcg cgatccgcgc ggcggccggt ccgggcaccg
     1921 tggccctgaa cgtgatcacg ggcccgtacg gccagacctt cggcaactgg ctgcgggacg
     1981 ccggcgccac ggtgcacgac ctgtcggtcc ccttccacac ggcggcgacc gccgagcaga
     2041 tccggcaggc cttcgccgaa cacccggaga tcgacttcgt gtcgctggtg cacgccgagg
     2101 cggcgaccgg caacaccaac ccggtcgccg agatcggcga ggcggtgcgg gcccagggcg
     2161 ccctcttcta cctggacgcg gtggcctccg tgggcgcgga gccggtgctg ccggacgcgt
     2221 ggggcgtgga cctgtgcgtg atcggggcgc agaaggcgat gggcggcccg gcgggcgtgt
     2281 cggcgatctc ggtgagcgag cgggcgtgga cccggatggc cgccaacccg aacgccccgc
     2341 gccgctccta cctgtccctg ctggactgga aggagcgctg gatcgacgcc ggccggacgg
     2401 cgctgccgca tgctccggcc cagctggaga tgctggcgct ggaggcgtgc ctggagcgga
     2461 tcgagtcggc cggcctgcag acggtgatgg cccggcaccg gcgcgccgcg ctggccacgc
     2521 gggccggtgc ggtggcgctg ggcgggggcc tggagccgta tgtgtacgag gagcgggacg
     2581 cggccccggt cgccacgacc ctgcgggtgc cgtcgggggt ggcggcctcg gacctggtgg
     2641 cccgcgcgct ggcgacggac ccgacgctgc cgctggcggc cggcggcggc gccctggcca
     2701 aggagatgat ccgcgtcaac cactacggcc ccgacgcgac cccgggcgcg gtgcagaact
     2761 ccctggccgc cctgggcgcc gcgctcgcgg agcaggggct gccggtggac ccggccgccg
     2821 cccgccgggc cgcggagctg gcctgggggt agtcagcccg gaacgcacgg aaaacggccc
     2881 gcgcacctca cgcgggccgt tttcacatcc tgaattccaa ttactttcac aatctccgcc
     2941 aatttaccgg acgatagctt cccatcttct tctgcccgct ttttgcagag ctaaacaagg
     3001 gactttgcgg acggatttcc gggggctttc gtgctgtgac acactccaca gcgagcacgc
     3061 tttgtcccga tatttcacgg ggcattccgg acatatcgcc tgggcgacga gtggcccccg
     3121 tgatcactcc cgtgcatttt cgaattcgcc tcgctaaatt cgtgccgcat gactgccgca
     3181 cccgcagacc tgctcatcga acggccggcg acgacggacg gagccgcgct ctggcgtctc
     3241 gccaaggaat cgaagaccct cgatctgaac tcctcctaca gctatgtgct gtggtgccgg
     3301 gacttcgccg gcacctcggc ggtggcgcgc ggtgcgggcg gggagccggt cggcttcgtc
     3361 accgggtacc tgcggccgga ccgcccccgc accctgctcg tctggcaggt cgccgtcgac
     3421 cccgcccacc ggggtcacgg catcgccgcc aagctgctgg acgggctgac cgcccggctc
     3481 accgccgaac gcggcatcac cgagctggag accaccatca cgcccggcaa caccgcctcc
     3541 gagcggctgt tcacctcgtt cgccgagcgg cacggcgccg gggtcagccg cgaggtgctg
     3601 ttccccgccg acctgttccc ggacggcccg cacgaccccg aagtcctcta ccgcatcggc
     3661 ccgctgaccg agttcaccgc ccactgagga gcgattgacc gtgaccatca cccagcccga
     3721 cctcagcgtc ttcgagaccc tcgaatccga ggtgcgcagc tactgccgcg gctggcccgt
     3781 cgtcttcgac cgggcgcagg gcagccggat gtacgacgag gacggccatg cgtacctcgg
     3841 ccgggcactt cttcgccggt gccggatcac tcaactacgg ccacaacaac ccggtgctga
     3901 aacgggcgtt gctcgactat ctggaacggg acggggtcac ccacggcctg gacatgtcga
     3961 ccacggccaa gcggtcgttc ctgcgggcgt tccaggagct ggtgctgcgt ccgcgtgatc
     4021 tgccgtacaa ggtgatgttc cccggcccga cgggcacgaa cgcggtggag tcggcgctga
     4081 agctggcgcg gaaggtgaag gggcgcgagg cgatcgtgtc gttcaccaac gccttccacg
     4141 gcatgtcgct gggttcgctc gcggtgaccg ggaacgcctt caagcgggcc ggtgccgggg
     4201 ttccgctggt gcacggcacg ccgatgccgt tcgacaacta cttcgacggt acggtgccgg
     4261 acttcctgtg gttcgagcgg ctgctggagg accaggggtc ggggctgaac cggccggccg
     4321 cggtgatcgt ggagaccgtg cagggcgagg gcgggatcaa cgtggcccgg ccggagtggc
     4381 tgcgggcgct gaaggatctg tgcgagcggc aggacatgct gctgatcgtc gacgacatcc
     4441 agatgggctg cggccgtacc ggtgccttct tctccttcga ggaggcgggg atcactccgg
     4501 acatcgtcac ggtgtcgaag tcgatcagcg ggtacgggct gccgatgtcg ctgtgcctgt
     4561 tccggcccga gctggacatc tgggagccgg gtgagcacaa cggcaccttc cgcggcaaca
     4621 accccgcctt cgtcaccgcc accgccgccc tggagaccta ctggaccgac ggcccggcga
     4681 tggagaagca gacccgcgcc cgcggcgaac agatcgagca ggccctgatc tccatcaccg
     4741 aggagaacct cgccgatgtg aaggagtacc gcggccgcgg gctcgtgtgg ggcctggagt
     4801 tccacgccaa ggaccgggcc gcgcgggtcg cgcggcgcgc cttcgaactg gggctgctca
     4861 tcgagacgtc cggccccgag ggcgaggtcg tcaagctgct gcccgccctc accgtgaccc
     4921 ccgacgagct ggacgagggc ctcagcgtcc tcgcccgcgc cgtccgggaa accgcctgaa
     4981 gcaaggagga gacgcacccc atgatcgtcc gatcgttcaa ggacatcgaa ggcaccgacc
     5041 ggcacgtcaa ggcgaagtcc ggcacctggg agagcaagcg gatcgtcctc gccaaggagc
     5101 gggtcggctt ctccctgcac gagacgatcc tgtacgccgg gaccgagacg tcgatgtggt
     5161 acgccaacca catcgaggcc gtcgtctgca ccaagggcga ggccgaactg accgaccacg
     5221 aggccgggaa gacgtacacg atcacccccg gcaccatgta cctcctcgac gggcacgagc
     5281 ggcacacgct gcgcgtcaag gaggacttcc actgcatctg cgtgttcaac ccgccggtca
     5341 ccggacggga ggaccacgac gagaacggcg tctacccgct gctcaccgag cccgaggagg
     5401 tgtgagacac gatgaccact gccaccgtca ccgatctgta ccccacccgc ggcaccaccg
     5461 aggtgaccgt cccgcgccag gacccggtcg tctggggctc cccggacacc cccggcccga
     5521 tcacgacggc cgacctccag gcgtacgagc gggacggctt cctcgccgtc gaccagctca
     5581 tcaccgagga cgaggtcgcg gtctacaagc gggagctgga ccggctggtg tccgacccgg
     5641 cgatccgcgc cgacgagcgg tcgatcgtgg agccgaagtc ccaggagatc cgctccgtct
     5701 tcgaggtgca ccggatcagc gaggtgttcg cgaacctggt gcgcgatccg cgcgtggtcg
     5761 gccgggcccg gcagatcctc ggctcggacg tgtacgtcca ccagtcccgg atcaacgtca
     5821 agccgggctt cggcgcgagc gggttctact ggcactccga cttcgagacc tggcacgccg
     5881 aggacggcct gccgaacatg cgcacggtgt ccgtgtcgat cgcgctgacc gagaactacg
     5941 acaccaacgg cgggctcatg atcatgcccg gctcgcacaa gacgttcctg ggctgcgccg
     6001 gtgccacgcc gaaggacaac tacaagaagt ccctgcagat gcaggacgcg ggcacgccgt
     6061 cggacgaggc gctgacctcg ctggcgagcg agtacggcat ccggctgttc accggcaggg
     6121 ccggctcggc gacctggttc gactgcaact gcatgcacgg ctccggcgac aacatcacgc
     6181 cgttcccgcg cagcaacgtc ttcatcgtgt tcaacagtgt ggagaacacg gcggtggagc
     6241 cgttcgcggc gccggtgcgg cggcctgagt tcatcggtgc gcgggacttc acgccggtga
     6301 agtagccccg cccccggaac caccgggccg ggcctcctcg tgtgggacgg ggggcccggc
     6361 ccggtcgtgt cagccggaca gcgccgccag cagccggtcc acgtccgcgg ccgtgttgta
     6421 gaggtggaag gccgcgcgca ggttgcccgc gcggtcggag acctcgacgc ccgccgcgct
     6481 caacgcgggc tgccgggagc cgagtccggg gaccgagacg atcgccgagc cgggcgcggg
     6541 caccggctcg tggccgaggt cggcgaggcc ggcgcggaac cggtcggcga gggcgaggtc
     6601 atgggcgtgg atcgcctcga cgccgagttc ctcgatcagt tcgagggagc ggcgcaggcc
     6661 ggcgaaggtg aacagggcgt gggtgaggtc gaaccgccgt gccgagtggg cgagttcggc
     6721 caccgggccg tagcagctgt cccacggccg ctcggccgcg acccagcccg cgagcaccgg
     6781 ggtcagtccg ccgaagtcct cggggacggt gaggaaggcg gcgccgtgcg ggcccatcag
     6841 ccacttgaag gagacggtgg cggtgaagtc gtaggcgtcc gccgccatcg gcagccagcc
     6901 ggcggcctgg gagaagtcga cgtaggtgcg ggcgccgtgg gcgcgggcgg cctcgcgcag
     6961 ggcgggcagg tcggcgatcc ggccgtcggc ggactgggcg gcgctgaccg cgacgagcgc
     7021 ggtgcccggc cggacggact cggcgatccg ctccagcggg accgtgcgca ccttgaggtc
     7081 gccgcggatg tggaacgggt tgagcacgga ggtgaagtcg tcctctgcgg tgaggacttc
     7141 ggcgcccgcc gggagggagg cggcgatcac accggtgtgc gcggcgacgg aggcgcccgc
     7201 cgcgacccgg gtggcgggga cgccggcgag ccgggcgtac gcggcccggc acacctccac
     7261 ctcctcgtac agcggcagca gcggccggcc ctccgcccgc agcagagccg cctcctggag
     7321 ggcggtgacg gtgcgggcgg gcagcaggcc ggtgctcgcg gtgttgaggt agctgccgcg
     7381 cgggcggaac tcggcgcgga cgaggcggtc gaaggtctcc gtggtctccg tggtctccat
     7441 gggtccactc tgcgtcccgg ggaaccaccc gtccattgcg gacttttcca tggtttccct
     7501 caggatcgct tatacgaccg cgccgacctg cggtttctca gtgctgcggg accgcacagc
     7561 cgtccgggcc gcaggcctcg gcgccgccct ggtcgacgag cttcagcggg gagcgctcgc
     7621 cccatgcctg ggtcagcgcc tgggtgaaga cctcggcggg ctgggcgccg gagacgccgt
     7681 acgtccggtc gaggacgaag aagggcacgc cggtggcgcc gagccgggcg gcctcgcgct
     7741 cgtcggcgcg gacgtcgtcg gcgtacgcgt ccgggtcggt gagcaccgcg cgggcggcct
     7801 cggcgtcgag tccggcctcc acggccagtt ccacgagccg ctcgtcgccc tcggtgaaga
     7861 cggaccgctc ctcggcgaag ttcgcccggt acagcagctg gagcagccgg tcctgccgtc
     7921 cgtgctcctt ggcgaagtgc agcaggcggt gcatgtcgaa ggtgttgccg tggtcccggt
     7981 cgcgggtgcg gtagtccagg ccctcggcgg cggcctgcgc gcccaggttg tcctcgccgg
     8041 cctgggcctg cgcctcgctc atgccgtact tcctggtcag catggtgatg accggctgca
     8101 catcgccctt ggcgcggccc gggtccagct cgaaggagcg gtgcaccacc tcgacctcgt
     8161 cgcggtgcgg gaaggcagcc agcgccttct cgaagcgggc cttgcccaca tagcaccagg
     8221 ggcaggcgat gtcgctccag atctcgacgc gcatgtcttc ggctctctcc aggtcgtacg
     8281 ggcacggggt ctctccccga ccggtacgtg aacgttcaaa cagaacgttt cattccccgg
     8341 gcggcacgga gaggcgcagg aagaggtggt ggtcgcccgg cgcgggcggg tgcgccgggt
     8401 ccgggaccca gccgtggcgg gcgtagaagg accgggcgcg cagattgtcc acatgcacgt
     8461 cgaggacggc gcagtgcctg ccgtcggcgc gccaccgcgc cacgcaggcc gcgtgcaggg
     8521 ccgtaccgat gccgcggcgc cagtggtcgg ggtcgacgtg gaactggaag agcttgaccg
     8581 tgccggccgg gccgtcctgc gggggccgga aggaggcgat gccgaggatc gtgccctcct
     8641 cgaccacgca cagcacctgc ccgtcggggc gctcgacggc accgcgccag gccgcggtcc
     8701 agtcgatgcc gtcgtccggc agtccgtcgg ggtagtacgt cgagcgggcc cggcggtgca
     8761 gtgcggcgat gttctccgct tcggcgggca gcgcgccgcg gatcgtccgg gggccccgct
     8821 cgtggtgact gatcacgcag cggaggacgc ggccgtcggc cggccggttg cgtggcggct
     8881 gaactgcgca cggtacgcgc tcggggtgag gccggtgcgg cggaccaggt gggcgcggag
     8941 cgagtcggcg gtgcccagac cgcaggcggc ggccacctgg tccatgggca gccgggtggt
     9001 ctccagcagt tccttggcgc gctcgatgcg ctggtgcagc agccactgca acgggctgac
     9061 cccgctctcg gcgtggaacc ggcgggtgag ggtgcgcacg gagaccccgg cgtgccgggc
     9121 caggtcggtc agggtgagcg gcttgtccag gttgcgcatg gcccagcccc gagtgtcggc
     9181 gcaggcggtg ccgcgctcgg gcggcagcgg ggtctgggtg aactgggtct ggccgccggg
     9241 ccggaccggg gcgaccaggg cgagccgggc gacctcgttg gcgacggcgg cgccgtagtc
     9301 cgtacggatg atgtgcaggc acaggtcgat gccggcggcg tagccggagg aggtcaggaa
     9361 cgggccgtcc tgggcgtaca ggacatcgcc ccggacgtcg atgtcggggt gccggcggcg
     9421 catctcctcg gcgtgtgccc agtaggtggt ggccttgcgg cccttgagga gtccggcctc
     9481 ggcgagcgtg aaggcaccgg agcagatgga ggcgatgcgc ttgcccctcc cggcggcctc
     9541 gcgcagcgcc tcgatcaccc gcgggtccgg ggcgaagggc tgcccggtgc cggcgacgag
     9601 cacggtgtcc gcctccccca gcgcctccag tccgcgcggc acgtgcagtt cgagcgtgcc
     9661 ggtggtgggc acgggcccgg ggtcgggcgc gcagatcacc acctcgtagc cggggcggcc
     9721 gtcgacccgc accttgtcga agagcagctc ggggatggcg aggttgaaca tcgacacggg
     9781 cggggacgcg acgaccgcga tacgcagcat ggccagaacc tccggatgca tggcattcag
     9841 gtcactactg tatgtcgcgg aagatcagca gcatgggggc atgcgaacga agcagacccc
     9901 acgtgtccgc caagtgccgg acgatacaag ggagttgaaa gctcccgctc ctgccctcac
     9961 cctggccgcc gccgtcctcg gcttcgcgtt gatcaccctc gacgcgtccg tggtgaccgt
    10021 ggccctcccg gccatgggct ccgccctcgg cggggggatg tccgggctcc agtgggtggt
    10081 ggacgcctac accctggcct tcgccgcgct catgctctcc acgggcgcct tcgcggaccg
    10141 ggcgggagcg agccgggcgt acgcgctcgg caccgccctg ttcaccctgg cctcggtggc
    10201 ctgcggcctg gccccgagcc tgccggcgct gatcggcgcg cgggtggcgc agggcgtcgc
    10261 ggcggccgtg gtgctgcccg cctcgctggc gctggtgcgg caggcgtacc cggagccggg
    10321 gcggcgggcc cgggcggtcg ccacctgggc ggccgggggc tcggtggccg tggcgctggg
    10381 cccggtggcg ggcggcgcgc tgacc
//
