LOCUS       NZ_AP024135            11053 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces sp. EAS-AB2608 strain NBRC 114648 chromosome.
ACCESSION   NZ_AP024135
VERSION     NZ_AP024135.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. EAS-AB2608
  ORGANISM  Streptomyces sp. EAS-AB2608
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Okubo,S., Ena,E., Okuda,A., Kozone,I., Hashimoto,J., Nishitsuji,Y.,
            Fujie,M., Satoh,N., Ikeda,H. and Shin-Ya,K.
  TITLE     Identification of functional cytochrome P450 and ferredoxin from
            Streptomyces sp. EAS-AB2608 by transcriptional analysis and their
            heterologous expression
  JOURNAL   Appl Microbiol Biotechnol 105 (10), 4177-4187 (2021)
   PUBMED   33944982
  REMARK    DOI:10.1007/s00253-021-11304-z
REFERENCE   2  (bases 1 to 9320785)
  AUTHORS   Okubo,S., Ena,E., Okuda,A., Kozone,I., Hashimoto,J., Nishitsuji,Y.,
            Fujie,M., Satoh,N., Ikeda,H. and Shinya,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Contact:Shinya Okubo Eisai Co., Ltd.;
            Tokodai 5-1-3, Tsukuba 300-2635, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Coverage                   :: 53x
            Sequencing Technology             :: Miseq; PacBio RS II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703895.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 09:16:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,174
            CDSs (total)                      :: 8,084
            Genes (coding)                    :: 7,890
            CDSs (with protein)               :: 7,890
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 87 of 194
            Pseudo Genes (incomplete)         :: 132 of 194
            Pseudo Genes (internal stop)      :: 10 of 194
            Pseudo Genes (multiple problems)  :: 32 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:41:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6245
            Orig. end                         :: 17298
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024135.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11053
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11053
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11053
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:6053](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6053
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(52..67)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.78"
                     /tool="antismash"
     gene            complement(1138..1719)
                     /locus_tag="K6K39_RS00040"
                     /old_locus_tag="EASAB2608_00008"
     CDS             complement(1138..1719)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.5"
                     /locus_tag="K6K39_RS00040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_00008"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_079081437.1"
                     /transl_table=11
                     /translation="MTNTERAARTRANLVLAAARRFDRHGYDGTALNHICAGAGVTLGA
                     LTFHFRSKAALASAVVDEGVRALQRIRTARPDTGRPLHDLTVLVLQVAGALQHDVLPRA
                     ATRLVEEGHVDSGWPGIWRAEVLRLLERAFVTGDLAPDVRPAAAAHLVMHVVEGAAHEA
                     RRAEAGGVWVASDVAEVWHAALGGLAAHPR"
     misc_feature    complement(2169..2184)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.18"
                     /tool="antismash"
     gene            complement(2274..3041)
                     /locus_tag="K6K39_RS00045"
                     /old_locus_tag="EASAB2608_00010"
     CDS             complement(2274..3041)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (10-171). E-value: 9e-16. Score:
                     50.2. Matches aSDomain:
                     nrpspksdomains_K6K39_RS00045_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677199.1"
                     /locus_tag="K6K39_RS00045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_00010"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_086703359.1"
                     /sec_met_domain="adh_short (E-value: 4.7e-35, bitscore:
                     120.1, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 6.6e-29, bitscore:
                     100.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPARGLLHGKTVLITGASSGIGAAAAELFAREEARVVLAARREDR
                     LLALVDRIGAAGGQAAAVVADVTRAEDMHAAVARAVERFGSLDAAFNNAGWTSTGTPLH
                     LMDDAEYDRIMDVNARGVWNAMRAQIPVMLESGGGSIVNTSSVAGLRATGSSAPYIAAK
                     HAVIGLSRAAADEYAAQRIRVNALAVGSTRTELMDQVLADTPALWKPFTERAIQQRLAD
                     PVEIAQTAAWLCSDRSSFTTGAVIPADGGWTAR"
     aSDomain        complement(2529..3011)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K6K39_RS00045_PKS_KR.1"
                     /evalue="9.00E-16"
                     /label="K6K39_RS00045_PKS_KR.1"
                     /locus_tag="K6K39_RS00045"
                     /protein_end="171"
                     /protein_start="10"
                     /score="50.2"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TVLITGASSGIGAAAAELFAREEARVVLAARREDRLLALVDRIGA
                     AGGQAAAVVADVTRAEDMHAAVARAVERFGSLDAAFNNAGWTSTGTPLHLMDDAEYDRI
                     MDVNARGVWNAMRAQIPVMLESGGGSIVNTSSVAGLRATGSSAPYIAAKHAVIGLSR"
     CDS_motif       complement(2916..3011)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_K6K39_RS00045_0001"
                     /evalue="6.90E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="K6K39_RS00045"
                     /protein_end="42"
                     /protein_start="10"
                     /score="18.7"
                     /tool="antismash"
                     /translation="TVLITGASSGIGAAAAELFAREEARVVLAARR"
     gene            complement(3350..4249)
                     /locus_tag="K6K39_RS00050"
                     /old_locus_tag="EASAB2608_00011"
     CDS             complement(3350..4249)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677198.1"
                     /locus_tag="K6K39_RS00050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_00011"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_221333832.1"
                     /transl_table=11
                     /translation="MTATAADPRHTGPRHTDPRHIVLTGATGQIAPLVARHLDARHRIT
                     LTSRTAPAAAQRAGVRHVACDYDDPGSLLHAFRGADALFVVTNDPTRPGHDRNIVRAAI
                     EAGVGHIVKLSAAAVHDSRAHDLITRWQRANEERIRDSGIPWTMIQPRSFMSHALAWAP
                     DIRRDGVVHALHPHSRNACIAPEDIAQVSARVLGDGGHHFQSYQLTGPQPLSAHEQCAV
                     LSELLGRPLVCRELSRQEAHARYARRYSPAMADALLAGAERQLAGAKAGTTDTVERLTG
                     RPATTFAEWAAGHLDRFA"
     gene            complement(4246..4869)
                     /locus_tag="K6K39_RS00055"
                     /old_locus_tag="EASAB2608_00012"
     CDS             complement(4246..4869)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677197.1"
                     /locus_tag="K6K39_RS00055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_00012"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_059256338.1"
                     /transl_table=11
                     /translation="MPTANRERAARTRQRILLAAAEVFDETGYERAAVTQIVERAGLTL
                     GALYFHFGSKQGLAEALMNAQPATIEPHLDSTGLQRLVDITLVWADRMRRDPILRAGVR
                     LAVEQGSHGMNDASSFEEWRSLMRDHLDVARKAGELREGVDPDRVARFVVGACTGMQVY
                     SQLATQRADLIQRVCDMWALLLPGIASAPVADGIQAGPERVPAS"
     gene            5001..6053
                     /locus_tag="K6K39_RS00060"
                     /old_locus_tag="EASAB2608_00013"
     CDS             5001..6053
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677196.1"
                     /locus_tag="K6K39_RS00060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_00013"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_260334975.1"
                     /sec_met_domain="AfsA (E-value: 3.7e-21, bitscore: 73.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGTVPRELVHKHAPHEVLLTDWTTHGHDRHTVRARWPRFHTFYA
                     SRNRRFDPLLFVETVRQTFPLLSHTAYGVPFGSHLVWDDFSFEVEPPGMLVPPGPADVV
                     LDVTCRDVRMRKGCLVSMTMHARALLDGRPVGSASTRFTNHSPAVYRRLRGEHGLAAKH
                     LRAVPLPPGLLPGTVGHLLPENVVLARTEHAGRWQLRVDLDHAVLFDHPVDHAPGMLLL
                     EALRQAAHATYPATDTMVTAMSVSFEKWVELDEAAWVSVRPLRRNVLRAVIEQSGAPCL
                     SGEVTVQAVPVPAAGSPRPLPPDGLEPLGPLGPLGPLGPSDEAPRSPGEAGREAVGAVA
                     PAPATGGRAG"
     gene            6558..7598
                     /locus_tag="K6K39_RS00065"
                     /old_locus_tag="EASAB2608_00014"
     CDS             6558..7598
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019984215.1"
                     /locus_tag="K6K39_RS00065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_00014"
                     /product="vWA domain-containing protein"
                     /protein_id="WP_059256336.1"
                     /transl_table=11
                     /translation="MSTSQNYINHVALVLDASSSMSHLSRKVVEVADQQIAYLARRSRE
                     LDQETRVTVYVFADTVECVIYDKDVLRMPSLKQLYRAGGMTALLAAALKSQRELAQTAQ
                     LYGDHSFLTFVLTDGQENASHRCPDAPARDPRELVGAVAEMIETQADNWTLAVLVPDQM
                     GRREAIQCGFPKDNVAIWDATSTQGLEEAGQVIQLATEKFMVGRTQGIRGSRAVFSVGA
                     DAVNKDTIEAAGLTPADPSTYQLLPVAREAAIRDWVVASGHTYRTGGAFYQLSKSEKIQ
                     ARKQIAVLEKKTDRVYTGPEARALLGLPDVEVRVKPDHNDDFTIFVQSTSVNRKLVANT
                     RLLLML"
     misc_feature    6579..6593
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     gene            complement(7658..8617)
                     /locus_tag="K6K39_RS00070"
                     /old_locus_tag="EASAB2608_00015"
     CDS             complement(7658..8617)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537240.1"
                     /locus_tag="K6K39_RS00070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_00015"
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="WP_260334977.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 5.2e-06, bitscore:
                     25.5, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 1.2e-26, bitscore: 92.3,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDQPPTQPDTPDSAGIWMLVRQHPAVLRRSTQELATALAAAHGP
                     RFALWHTDELLFGVRDGRLTLHTLGGTDLPAPEVVCVRQVAGPMHTDREVTLLRHLERM
                     GSTLINSLDAQLKCRNKIWQLQELALAGLPVPDTLSYATAPLEGVVRSPHLATPCVVKS
                     VNGAKGTRVFLAPDPHLLRDVAGSLTHDTPFLFQQHIAHSHGRTLRVVVVDGEPVEAVL
                     HTSRHHTLAANIANGGAAALCTGHHPRAEQLAVQAAAALGLAIAGVDLLFTSPDTYTVC
                     EVNAVPGWRPEMKAVTPAITACITRHLTARPRTTAHHP"
     gene            complement(8685..9350)
                     /locus_tag="K6K39_RS00075"
                     /old_locus_tag="EASAB2608_00016"
     CDS             complement(8685..9350)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043878.1"
                     /locus_tag="K6K39_RS00075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EASAB2608_00016"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_059256508.1"
                     /transl_table=11
                     /translation="MTGAHDGETPETAACRPAHRRSASRTRTALRAAAAFRFGKYGYEG
                     TSVRDIAGDVGVDAALVYRYFGSKEALFNDVVGTDKIFEPLLHEPVETIPDWFCALLLG
                     PPSDGDFPHPVLTVLRSSGRDEAVARLRADFTEVFTKRLAARLTGPDADLRAELLAAWM
                     LGTSLMRTEIRPPALASAPDATLERYLRAGIEILLGPSPRHDERTTGTGDPHHTPDDR"
ORIGIN
        1 tacggcggct ggcggaatcc gtcaccgact cccccacggc ctcgcaggcc atgcaccagt
       61 tcgtcaacgc cagcccctgg gactggctgg cggcacgcca gggcctgctg cgctgggtgg
      121 agcagcaggg cggcccggcg gtgacggcct ggaccctggc gcccgccttc atccccaagc
      181 gcggccggca ctccgccggc gtccaccgca gattcgaccc cctgctgggc cgggtgtcca
      241 actgccagct cggcatggtc atgctggcca cgggcgcgca cggcccgctg ccggtcgact
      301 ggagcctgta catcccccac ggatgggcgg cggactcccc gctgcgctcc aaggtgcggg
      361 tgcccgacga cgcctgcggg cccctgtggt cacaggccgc gaaactcctg cgccgcgccg
      421 tgtcgtggtc ccccggccgg caggcccccg tcaccgcgga cctgtcctgg atgcccgacc
      481 acgaggcctt cctcgcctgc ctggcacgca gcgagcagcc cttcctggcc gaagtccccg
      541 acaccgtgca cgtcaggcgt gtgacatcac ggccggccga cctgccggcg ctcaccgtcc
      601 ggcagctgct caccgaccgc gggaccgcgg acgcggcaga acgccagcac ctgctgtccc
      661 tcccggtggc ccttcccggc gcccccgccg gacgcgcccg gctgcggctg ctggcccagc
      721 gccgccccga cggctcggca cggacctggc tgacgaacct gctgaaccgg ccgctgcccg
      781 acctgctgcc gctgatgcgg cagccccggc aggccgcgac cgtggtcacc accctgagcg
      841 gggacttcgg actccaggac ttcgaaggcc ggtccttccc cggctggcac caccacacca
      901 ccctggtctc cgccgccttc acctactccc aggtccgcgc cggctccgcg tgagccgaag
      961 acccggctcg gcgtgagcag aaggctctgc tcctggaggg ggctgaagac gcccggccgg
     1021 atttttcccg gccgggcgcg cgcccgcgcc gccgcggccg gcggccggca gtcccgcccg
     1081 gcggcccgtg cgttcggcgg cccgtgcgtt cggcgggcgc gcaccgcccg cacgtgctca
     1141 gcgcgggtgg gcggcgaggc cgccgagcgc ggcgtgccag acctcggcga cgtcggaagc
     1201 cacccagaca cccccggcct cggcacgccg cgcctcgtgc gcggctccct ccaccacgtg
     1261 catgaccaga tgcgcggcgg cggccggccg cacgtccggg gccaggtcac cggtgacgaa
     1321 ggccctctcc agcagccgca gcacctccgc gcgccagatc ccgggccagc ccgagtcgac
     1381 gtgcccctcc tcgaccaggc gcgtggcagc ccgcggcaga acgtcgtgct gcagcgctcc
     1441 ggccacctgc agcacgagga cggtcaggtc gtggaggggc cggccggtgt cggggcgcgc
     1501 cgtgcggatg cgctgcagcg cgcggacgcc ctcgtccacc accgccgacg ccagcgccgc
     1561 cttgctgcgg aagtggaagg tgagggcccc gagcgtgacg cctgctccgg cacagatgtg
     1621 gttgagcgcc gtaccgtcgt agccgtgccg gtcgaagcgg cgtgctgctg ccaggacgag
     1681 gttcgcgcgg gtccgcgcgg ctcgttccgt gttcgtcatg acgggacctc gaggagcgtg
     1741 acgacaggcg acccctcccc cgcggggccg gggtgcacat gtccggaagg ggcgcgccgc
     1801 aggcggtgcc ctcgcgcgcg cgaggcggcc cgtgcgcggg accgggcgcg cgggcacgcg
     1861 gaggtgccgg gagaggcggc gtgtccctcc cggccggcgc ccgccgccgc gccgccggag
     1921 cacacgcgcg cgcccggccc gctggcggac gtaccggcgg ccgtagcggt cgtattggtg
     1981 gtcgtagcgg ccccgaccgg cccccgcgag gaggggccgc cggagcgtgc gggttctgcc
     2041 cggccccgtg ccccgccccg gctgcggtgt gtcatgacga ggccagtcag gttgctcacg
     2101 taccgtctcc agctctggct gcccgtggcc gcgggcggcg ggcccgcggc ggccgctcga
     2161 cgggcggtcc gaccatacgg tgagcgccgg gcgacgagca cgggaacggg gcaagtcccg
     2221 aacgttctgg gcaagttggc tggtggcgtg cggtgggtgg cgctgcggtg cggctagcgg
     2281 gcggtccatc ctccgtcggc ggggatgacc gcgcccgtgg tgaacgacga acggtccgag
     2341 cagagccagg cggccgtctg ggcgatctcg acggggtccg ccaggcgctg ctggatggca
     2401 cgctcggtga agggcttcca cagcgcgggg gtgtcggcga gcacctggtc catcagctcg
     2461 gtgcgggtgc tgcccaccgc gagcgcgttg acgcggatgc gctgggccgc gtactcgtcg
     2521 gccgcggccc ggctcaggcc gatgacggcg tgcttggcgg ctatgtaggg ggcggagctg
     2581 ccggtggcgc ggagtccggc gacgctggag gtgttcacga tcgagccgcc gccgctttcg
     2641 agcatcacgg gtatctgtgc ccgcatggcg ttccacacgc cgcgggcgtt gacgtccatg
     2701 atgcggtcgt actccgcgtc gtccatcagg tgcagggggg ttccggtgga ggtccacccc
     2761 gcgttgttga aggcggcgtc caggctgccg aacctctcca ccgccctcgc cacggcggcg
     2821 tgcatgtcct cggcgcgggt cacatcggcc accacggcgg cggcctgccc gcccgccgcg
     2881 ccgatcctgt ccacgagcgc aagcagccgg tcctcacggc gggcggccag caccacgcgg
     2941 gcctcctccc gcgcgaacag ctccgccgcc gctgcgccga tgccgctgct cgctccggtg
     3001 atcaggactg tcttgccgtg cagcaggccc cgtgcgggca tgggtctcct tagacgtgga
     3061 cttcacaccc ccgggacagg ggtgcgtgtg tgccggggcg cggccgggat acggccgggc
     3121 cttcgagatc gtcacgcgaa gggggaccgg cccgcaagcc cggcccggtc tggggtgcgg
     3181 tctgctgacc atgtgtcaga gacgcggcaa cggtggcgag ttgggcggca gggggcggcg
     3241 ggccggggtg cgggtggccg gccggggggc tcccgcacct tccggctccc gccccctccc
     3301 ggctcccgcc ccctcccggc tcccgccccc ttccggcttc cgctccgtct cacgcgaagc
     3361 ggtcgaggtg gccggccgcc cactcggcga aggtggtcgc gggccggccc gtgaggcgtt
     3421 cgaccgtgtc cgtggtgccg gccttggcac cggccagctg ccgctcggcg ccggcgagca
     3481 gggcgtcggc catcgccggc gagtagcggc gcgcgtagcg cgcgtgcgcc tcctggcggg
     3541 acagctcccg gcagaccagg ggacggccga gcagttcgga caggacggcg cactgttcgt
     3601 gggccgacag cggctgcggg ccggtgagct ggtaggactg gaagtggtgt ccgccatcgc
     3661 ccaggacgcg ggcggacacc tgcgcgatgt cctccggcgc gatgcaggcg ttgcggctgt
     3721 gcgggtgcaa cgcgtggacg acgccgtcgc ggcggatgtc cggcgcccag gccagggcgt
     3781 gggacatgaa cgagcgcggc tggatcatgg tccacgggat gccgctgtcc ctgatgcgtt
     3841 cctcgttggc gcgctgccag cgggtgatca ggtcgtgcgc ccggctgtcg tgcaccgccg
     3901 cggcggagag tttgacgatg tggcccacgc cggcctcgat ggccgcccgc acgatgttgc
     3961 ggtcgtggcc ggggcgcgtg gggtcgttcg tgacgacgaa gagggcgtcg gcgccgcgga
     4021 acgcgtggag gaggctgccg gggtcgtcgt agtcgcaggc gacgtggcgg acgcccgcac
     4081 gctgcgcggc ggcgggagcc gtgcggctgg tgagggtgat gcggtggcgg gcgtccaggt
     4141 gccgggccac cagcggggcg atctgcccgg tggccccggt cagcacgatg tgccgggggt
     4201 cggtgtgccg ggggccggtg tgccgggggt cggctgccgt cgcggtcatg aggcgggcac
     4261 gcgctcgggg ccggcctgga tgccgtccgc caccggcgcc gaggcgatgc cgggcaggag
     4321 cagcgcccac atgtcgcaca cgcgctggat gaggtcggcg cgctgggtgg cgagctggga
     4381 gtagacctgc atgccggtgc aggcgccgac gacgaaacgg gccacccggt cggggtcgac
     4441 gccctcgcgc agctcccccg ccttccgggc gacgtcgagg tggtcgcgca tgagcgagcg
     4501 ccattcctcg aaggaggagg cgtcgttcat gccgtggctg ccctgctcga cggcgagacg
     4561 gacgcccgcg cgcaggatcg ggtcgcgccg catgcggtcc gcccacacca gggtgatgtc
     4621 caccaggcgc tgcagcccgg tcgagtccag gtggggctct atcgtcgccg gctgggcgtt
     4681 catcagtgct tcggcgaggc cctgcttgga accgaagtgg aagtacagcg cgccgagggt
     4741 gagccccgcc cgttccacga tctgggtcac cgccgccctc tcgtaaccgg tctcgtcgaa
     4801 gacctcggcc gcggccagca ggatcctctg tcgggtccga gcggctcgct ccctgttggc
     4861 agttggcacc cgcgatcact cctcgacaga catagataac gtactttatg ttagccgcac
     4921 tgctgccgag ctgaccgtcc gttcaggggg aaagggctgg catgtccaac tcttatggct
     4981 taggacacga cgtagcgcgg gtgaccggaa cggttccccg ggaactcgtc cacaagcacg
     5041 cgccccacga ggtgctgctg accgactgga ccacccacgg ccacgaccgg cacaccgtcc
     5101 gcgcccggtg gccgcgcttc cacaccttct acgcgtcccg caaccggcgc ttcgacccgc
     5161 tgctgttcgt cgaaacggtc cgccagacgt tcccgctgct gtcgcacacc gcctacggtg
     5221 tgcccttcgg cagccatctg gtctgggacg acttcagctt cgaggtcgag ccgccgggca
     5281 tgctggtccc gcccggcccg gccgatgtcg tgctggacgt cacgtgccgg gacgtgcgga
     5341 tgaggaaggg ctgcctggtc tcgatgacca tgcacgcccg ggcccttctg gacggccgcc
     5401 cggtcggcag cgccagcacc cgcttcacca accactcccc cgccgtctac cggcggctgc
     5461 gcggcgagca cgggctcgcc gcgaagcacc tgcgggccgt gccgctcccg cccggcctgc
     5521 tgcccggcac ggtgggacac ctcctgcccg agaacgtggt cctggcgcgc accgagcacg
     5581 ccggccggtg gcagctgcgc gtcgatctgg accacgccgt cctgttcgac cacccggtcg
     5641 accacgctcc cggcatgctg ctgctggaag ccctgcgcca ggccgcccac gcgacctacc
     5701 cggccacgga caccatggtg acggcgatgt ccgtgtcctt cgagaagtgg gtggaactgg
     5761 acgaagcggc ctgggtctcg gtgcggcccc tgcgccgcaa cgtgctgcgg gccgtcatcg
     5821 agcagtcggg cgcgccctgc ctgagcggtg aggtcacggt ccaggccgtg ccggtcccgg
     5881 cggcgggatc gccccgcccg ctcccaccag acgggctcga accgctgggg ccgctggggc
     5941 cgctcgggcc gctcgggccg tcggacgagg cgccgcgctc gccgggtgaa gccggccggg
     6001 aggctgtggg ggccgtcgca ccggcgcctg ccacgggggg ccgggccggc tgatcccggc
     6061 accgggcctg gtggtgacac agggtcaggg cggcgcgagg ggcgcaccgg ccggacccgt
     6121 ggggcaagcc gcggaagccg cgcagaccgc ctttgatgcg ggcggtctcc gcggctgtcg
     6181 gcatgcccgg ggcgcgcatg gggtccggcc cgcgggaccg gcgcggccgc ggggaggttt
     6241 cccggtacgg catccccggc ggcccgggca ccccgggcgg acacccctcc ggcgggcccc
     6301 tccggcaggc ccctgcggcg gtgctgccgc cgggcgcccc gaggtgcgcg gcggcggcac
     6361 ctggttcccc tgcggcggta gggccgttcg tccggccacc tggccgcgtc ggggcatgcg
     6421 gtcggcgggt ggtgtgtgcg gtgccgggcc gcggcggccg ccggttgcgc ggtccccggc
     6481 cgggacggaa gccatgatcg attgtcagtg gcgggtgcga cagtaggcac atcgagccgg
     6541 gaagaggggg aaccgtcatg tccaccagcc agaactacat caaccacgtc gctctcgtgc
     6601 tggacgccag ttcgtccatg tcgcacctga gccgcaaggt cgtcgaggtc gccgaccagc
     6661 agatcgccta tctcgcccgc cgctccaggg aactggacca ggagacccgg gtcacggtgt
     6721 acgtcttcgc ggacacggtg gagtgcgtca tctacgacaa ggacgtgctg cgcatgccgt
     6781 cgctgaagca gctgtaccgg gccgggggga tgacggcgct gctggcggcc gcgctgaagt
     6841 cccagcggga gctggcgcag accgcccagc tctacggtga ccacagcttc ctgacgttcg
     6901 tgctgaccga cgggcaggag aacgccagcc accgctgccc ggacgccccg gccagggatc
     6961 cgcgggaact ggtcggcgcg gtggccgaga tgatagagac gcaggcggac aactggacac
     7021 tggccgtgct ggtgccggac cagatgggcc ggcgcgaggc catccagtgc ggcttcccga
     7081 aggacaacgt ggccatctgg gacgccacca gcacgcaggg gctggaggag gccgggcagg
     7141 tcatccagct ggccaccgag aagttcatgg tgggccgcac ccagggcatc cggggatcgc
     7201 gggcggtgtt ctccgtgggc gcggacgcgg tcaacaagga caccatcgag gcggccggcc
     7261 tcaccccggc ggatccctcg acgtaccagc tgctcccggt ggcccgtgag gcggcgatcc
     7321 gggactgggt cgtcgccagc gggcacacct accgcaccgg cggggcgttc taccagctga
     7381 gcaagtcgga gaagatccag gcgcgcaagc agatcgcggt gctggagaag aagacggacc
     7441 gcgtctacac cgggcccgag gcccgggccc tgctcggcct gccggacgtc gaagtccgcg
     7501 tcaagccgga ccacaacgac gacttcacca tcttcgtgca gagcaccagc gtgaaccgca
     7561 agctcgtggc gaacacacgc ctgctgctca tgctctgaga cccgcccccg ggggggccgc
     7621 gggccgcgtt ccggccgccg cgggatcccc ccgggtgcta ggggtggtgg gccgtggtgc
     7681 gggggcgggc ggtcaggtgg cgggtgatgc aggcggtgat ggcgggggtg acggccttca
     7741 tctccgggcg ccagccgggg acggcgttca cctcgcagac ggtgtaggtg tcgggggagg
     7801 tgaacagcag gtcgaccccg gcgatggcca ggcccagggc ggcggccgcc tggaccgcca
     7861 gttgctcggc ccgggggtgg tggccggtgc acagggcggc ggcgccgccg ttggcgatgt
     7921 tggcggcgag ggtgtggtgg cgggaggtgt gcaggacggc ctcgacgggc tcgccgtcca
     7981 cgacgacgac ccgcagggtg cggccgtgcg agtgggcgat gtgctgctgg aagaggaagg
     8041 gggtgtcgtg ggtgaggctg ccggccacgt cacgcagcag atgggggtcc ggggcgagga
     8101 agacgcgggt gcccttggcg ccgttgacgg acttcacgac acacggcgtg gccaggtggg
     8161 ggctgcggac gacgccttcg aggggggcgg tggcgtagga gagggtgtcg gggacgggga
     8221 gtccggccag ggcgagttcc tggagctgcc agatcttgtt gcggcacttc agctgggcgt
     8281 cgaggctgtt gatcagcgtg ctgcccatcc gttcgaggtg gcgcagcagg gtgacttcgc
     8341 ggtcggtgtg catggggccg gcgacctggc gtacgcagac cacttcgggt gcgggcaggt
     8401 cggtgccgcc cagggtgtgc agggtcagcc ggccgtcgcg gacgccgaag aggagttcgt
     8461 cggtgtgcca cagggcgaag cgggggccgt gggccgcggc gagcgcggtg gcgagttcct
     8521 gggtggagcg gcgcaggacg gcggggtgct ggcggaccag catccagatg ccggcgctgt
     8581 ccggggtgtc cggctgggtg gggggctggt cggtcacggt ggctcctcgc cgaacggggt
     8641 gtgcgtctct tcctcgaagg ccggccccct cgggccgcgg tgggtcagcg gtcgtcgggg
     8701 gtgtggtgcg ggtcgccggt cccggtggtc cgctcgtcgt gccggggtga tgggccgagc
     8761 aggatctcga tgcctgcgcg caggtagcgt tccagtgtgg cgtccggtgc ggaggccagg
     8821 gccggggggc gtatctcggt gcgcatcagg ctggtgccga gcatccaggc cgccaggagt
     8881 tcggcgcgca ggtcggcgtc gggtccggtg aggcgggcgg cgaggcgttt ggtgaagacc
     8941 tccgtgaagt ccgcccgcag ccgggcgacg gcctcgtcgc ggccggagga gcgcaggacc
     9001 gtcagcacgg ggtgggggaa gtcgccgtcg ctgggcggtc ccagcagcag tgcgcagaac
     9061 cagtcgggga tcgtctcgac cggttcgtgc agcagcggtt cgaatatctt gtcggtgccc
     9121 acgacgtcgt tgaacagtgc ttccttcgag ccgaagtagc ggtagacgag ggcggcgtcg
     9181 acgccgacgt cgccggcgat gtcgcggacg ctggtgcctt cgtagccgta cttgccgaag
     9241 cggaaggccg cggcggccct gagggcggtg cgtgtgcgtg aggccgagcg cctgtgggca
     9301 ggccggcagg ccgccgtctc cggcgtctcg ccgtcgtgtg ctcccgtcac cgtcaccgca
     9361 cctccgtcat ccgtcatccg tcatccgccg tccgtcaact gccgtccgtc gactgccgtc
     9421 caccgtccgc cgcccggcat ccgccgtcca ccgcccggca tccgccgccc ggcatccgcc
     9481 gtccaccgtc cgccgcccgg cgtcggccgt ccggcgtcgg ccgtccggcg tctgccgtcc
     9541 gcggtcatcg cgtcgactgc cccatgttcc agtctggagg ggctgtctca aagtcaccga
     9601 ccgatgtcac caatcgttga cttgtgttcg cggtgcatgc catacctgaa gacaggtcaa
     9661 cgaccgacga cttctccatc tcgctgcatc cgggccgtac gacggagccg gcccgttcca
     9721 ggtcgcccgc gcggcgcggc ggcggcgcgc gtcctcgacg gcgcgcggcc ggagccgacg
     9781 cgcgggcccc accgaccgga aatggtgatc ttctgtgtct gcacgacatg tcgccgtgat
     9841 cggaaccggc tacgtcggac tgaccaccgg tgcctgcctg gccttcctgg ggcactctgt
     9901 ggtgtgcgcc gacgccgacc cgcacaaggt cgagcggctg cgcggggccc gggtcgacat
     9961 cctggaaccc ggtctgccgg agagggtgcg cgagggcctg gaggcgggcc ggctggagtt
    10021 caccggcgac accgcggccg ccgtcgccga ggccgaggtg gtcttcctgt gcctgcccac
    10081 ccccatggga gcgggcggcg cggccgatct cgccgccgtg gaggcggtcg ccgggcaggt
    10141 ccgtgacgtg ctgcggcccg gctgtgtcgt cgtcaacaag tcgaccgtgc cggtcgggac
    10201 ctgcgagcgc gtcgccgcgc tgctgggccg ggcggacgtg agcgtggtga gcaacccgga
    10261 gttcctgcgc gagggccgcg ccgtcgagga cttcctgcac ccggaccgca tcgtgatcgg
    10321 cgccgccgac caggacgccg cccggcgcgt ggccggcctg tacgccgcgc tggacgcgcc
    10381 tttggtgctc accgatcccg ccagtgccga actgaccaag tacgcggcga acttcttcct
    10441 cgcgatgaag ctgtccttcg ccaacaacct cgccgtcctg tgcgagcgct tcggcgccga
    10501 cgtcgccgat gtcaccgagg gcatcgggcg tgatccgcgc atcggcccgg cgttcctgcg
    10561 gcccgggccg ggctggggcg gttcctgcct gcccaaggac acccacgccc tgctgaccgt
    10621 gtgcgaccgc tccggcgtgg acttcccgct gctgcgcgcc accatcgaga ccaacgtcgc
    10681 ccaccagcgc cggctcgtcg aacgggtcgc cgccgtgtgc gggcgcggcg cggagggctc
    10741 gctgcacggc gtacggctcg ccctgtacgg gctgaccttc aaggccggca ccagcgacct
    10801 gcgcgactcc cccgcgctcg ccgtcgcgcg gctgctgcgc gagcggggtg cggaactgac
    10861 cgcctacgac ccggcgttgc gcgaggagcg gcccgatctg agtgatctgg tcgacctcgt
    10921 cgacgatctg gtgcgggccg cggagggggc cgcggcctgc ctggtcctga ccgagtggcc
    10981 gcagctgcgg gatgtggact gggcggcggt cgcggcgtgc atgagcggcc atgaggtcca
    11041 cgactggcgc aac
//
