LOCUS       NZ_CP077098            24231 bp    DNA     circular CON 03-MAR-2024
DEFINITION  Staphylococcus aureus strain RGB-095930 chromosome, complete genome.
ACCESSION   NZ_CP077098
VERSION     NZ_CP077098.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2904556)
  AUTHORS   Burgold-Voigt,S., Monecke,S., Simbeck,A., Holzmann,T., Kieninger,B.,
            Liebler-Tenorio,E.M., Braun,S.D., Collatz,M., Diezel,C., Muller,E.,
            Schneider-Brachert,W. and Ehricht,R.
  TITLE     Characterisation and Molecular Analysis of an Unusual Chimeric
            Methicillin Resistant Staphylococcus Aureus Strain and its
            Bacteriophages
  JOURNAL   Front Genet 12, 723958 (2021)
   PUBMED   34868203
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904556)
  AUTHORS   Burgold-Voigt,S., Braun,S.D., Kieninger,B. and Monecke,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2021) Optisch-Molekulare Diagnostik und
            Systemtechnologien, Leibniz Institute of Photonic Technology (IPHT),
            Philosophenweg 7, Jena 07743, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-FEB-2021
            Assembly Method                   :: Flye v. 2.8-b1674
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 169.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiniSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 14:14:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,951
            CDSs (total)                      :: 2,873
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 43 of 101
            Pseudo Genes (incomplete)         :: 55 of 101
            Pseudo Genes (internal stop)      :: 22 of 101
            Pseudo Genes (multiple problems)  :: 17 of 101
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:14:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1934599
            Orig. end                         :: 1958830
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077098.1.
            Bacteria and source DNA available from Prof. Wulf
            Schneider-Brachert, Department of Infection Prevention and
            Infectious Diseases, University Hospital Regensburg, Regensburg,
            Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..24231
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lanthipeptide-class-i"
                     /region_number="5"
                     /rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..24231
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..24231
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:14231](-)"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..14231)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     misc_feature    complement(137..148)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 20.11"
                     /tool="antismash"
     gene            complement(741..1460)
                     /gene="splD"
                     /locus_tag="KSF82_RS09415"
                     /old_locus_tag="KSF82_09405"
     CDS             complement(741..1460)
                     /EC_number="3.4.21.-"
                     /codon_start=1
                     /gene="splD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /locus_tag="KSF82_RS09415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09405"
                     /product="serine protease SplD"
                     /protein_id="WP_001038704.1"
                     /transl_table=11
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLITN
                     TNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYKVT
                     KIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPNGNK
                     LQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGESTRSFA
                     VYFSPEIKKFIADNLDK"
     gene            complement(1581..2300)
                     /gene="splC"
                     /locus_tag="KSF82_RS09420"
                     /old_locus_tag="KSF82_09410"
     CDS             complement(1581..2300)
                     /EC_number="3.4.21.-"
                     /codon_start=1
                     /gene="splC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /locus_tag="KSF82_RS09420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09410"
                     /product="serine protease SplC"
                     /protein_id="WP_070006255.1"
                     /transl_table=11
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVKD
                     TNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKIKS
                     ISDYPGDEDISVMNIEEQAVERGPKGFIFNENVQAFNFAKDAKVDDKIKVIGYPLPAQN
                     SFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSEYNGA
                     VYFTPQIKDFIQKHIEQ"
     gene            complement(2358..3080)
                     /gene="splB"
                     /locus_tag="KSF82_RS09425"
                     /old_locus_tag="KSF82_09415"
     CDS             complement(2358..3080)
                     /EC_number="3.4.21.-"
                     /codon_start=1
                     /gene="splB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /locus_tag="KSF82_RS09425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09415"
                     /product="serine protease SplB"
                     /protein_id="WP_001039447.1"
                     /transl_table=11
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVKD
                     TNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSIKK
                     IINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHPYKN
                     KYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDNRNAY
                     GVYFTPEIKKFIAENIDK"
     misc_feature    complement(3147..3158)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.53"
                     /tool="antismash"
     gene            complement(3205..3912)
                     /gene="splA"
                     /locus_tag="KSF82_RS09430"
                     /old_locus_tag="KSF82_09420"
     CDS             complement(3205..3912)
                     /EC_number="3.4.21.-"
                     /codon_start=1
                     /gene="splA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /locus_tag="KSF82_RS09430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09420"
                     /product="serine protease SplA"
                     /protein_id="WP_001039435.1"
                     /transl_table=11
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITDA
                     TKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDVKD
                     IVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTKYKM
                     FESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFGVYFT
                     PQLKEFIQNNIEK"
     misc_feature    complement(4840..4851)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.09"
                     /tool="antismash"
     gene            4876..5442
                     /locus_tag="KSF82_RS09435"
                     /old_locus_tag="KSF82_09425"
     CDS             4876..5442
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /locus_tag="KSF82_RS09435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09425"
                     /product="DUF4888 domain-containing protein"
                     /protein_id="WP_001092763.1"
                     /transl_table=11
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEEK
                     MTDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSIDF
                     KGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSPVSG
                     KVGEGVTSGGENTLTKRFINKITID"
     gene            complement(5916..6614)
                     /locus_tag="KSF82_RS09440"
                     /old_locus_tag="KSF82_09430"
     CDS             complement(5916..6614)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /locus_tag="KSF82_RS09440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09430"
                     /product="hypothetical protein"
                     /protein_id="WP_000586140.1"
                     /transl_table=11
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNGR
                     QISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNASMIFTYTITA
                     ICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMAIVVGVLMFI
                     FNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITLLLAVLLFKGF
                     NKWSGRTIKD"
     gene            complement(6611..7372)
                     /locus_tag="KSF82_RS09445"
                     /old_locus_tag="KSF82_09435"
     CDS             complement(6611..7372)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /locus_tag="KSF82_RS09445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09435"
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="WP_000581552.1"
                     /transl_table=11
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGTA
                     NSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIRAKIF
                     ILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLLVTILLMYVVSLPLIPLNFLLTRYF
                     GVFVSILINLVLSVICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGLVVNHNSKYFN
                     DLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
     gene            complement(7369..8061)
                     /locus_tag="KSF82_RS09450"
                     /old_locus_tag="KSF82_09440"
     CDS             complement(7369..8061)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /locus_tag="KSF82_RS09450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09440"
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="WP_001096791.1"
                     /transl_table=11
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTTL
                     MKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNESVD
                     FSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDPYGI
                     QELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLEEIFF
                     KITKGDYK"
     gene            complement(8084..9457)
                     /locus_tag="KSF82_RS09455"
                     /old_locus_tag="KSF82_09445"
     CDS             complement(8084..9457)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /locus_tag="KSF82_RS09455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09445"
                     /product="S8 family serine peptidase"
                     /protein_id="WP_000691541.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 2.2e-43, bitscore:
                     146.1, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFNK
                     LVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDRTS
                     NESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKNLVP
                     LNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGSKKTE
                     LLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINYAKKKK
                     SIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSNLSEFSN
                     FGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGTSLATPKV
                     SGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALNVANQKAS"
     gene            complement(9467..9985)
                     /locus_tag="KSF82_RS09460"
                     /old_locus_tag="KSF82_09450"
     CDS             complement(9467..9985)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Flavoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /locus_tag="KSF82_RS09460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09450"
                     /product="phosphopantothenate--cysteine ligase family
                     flavoprotein"
                     /protein_id="WP_000504439.1"
                     /sec_met_domain="Flavoprotein (E-value: 5.8e-29, bitscore:
                     98.0, seeds: 143, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFIN
                     GEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTICH
                     TAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNVVAP
                     EPYKVLEFI"
     gene            complement(10001..11245)
                     /locus_tag="KSF82_RS09465"
                     /old_locus_tag="KSF82_09455"
     CDS             complement(10001..11245)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140: Lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /locus_tag="KSF82_RS09465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09455"
                     /product="lanthionine synthetase C family protein"
                     /protein_id="WP_000566596.1"
                     /sec_met_domain="LANC_like (E-value: 2.1e-85, bitscore:
                     284.0, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGII
                     LFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGKNY
                     QNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNVKAL
                     KSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFALCVIK
                     GITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNTGVMTT
                     LFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNLNFELNE
                     VSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLTLMTLKNT
                     KILNEKNWTNAFLIS"
     gene            complement(11238..14231)
                     /locus_tag="KSF82_RS09470"
                     /old_locus_tag="KSF82_09460"
     CDS             complement(11238..14231)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /locus_tag="KSF82_RS09470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09460"
                     /product="lantibiotic dehydratase"
                     /protein_id="WP_001092605.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.1e-151,
                     bitscore: 503.6, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 2.5e-13,
                     bitscore: 48.0, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 4.4e-23, bitscore:
                     79.7, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMKE
                     SILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHFVN
                     EPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYLNEQ
                     SAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLAEKINQEFEINDITKVKVYIH
                     NLVSKEIIYSTIRPPLSYSDNLNYILNKLSLHNDDFVKKIREIQKLILAYEKTEIGFGE
                     ELYKDIIHHMKALFKCKNYLQIDTKIDMINNYLHQDIATNISEAAYLLWLLSRNNIGFT
                     DLKVLHNRFIEKYGFEQLVNIKDLISDITGFGTTIFQEEETDGNNIVMLKQKFLHALRN
                     NDEIVINEKDVESLINDNEINHYHAPMSADVYAELYLGRFYNQYNELIVISPLTASFNA
                     GATFGRFHHLIDTETLAKLEHEKGHYYQKMICDDNVEMISINNIPKYPRNHNVLTNHDS
                     YEYSLNLGSSNSYSKYELTLDDIYVGATFNKLYLYSSQLNKRVLFESNNMYNFLKECNL
                     YRLLREISMESVKCIEPMNDVSIDSFSYSPRIRYKNVILKPAYWKINEMVLPLPKNEEW
                     DQQFLKYQEQFNIPNIVNLVYGDNKLLLNLSLANHRYLLMKEYKKHKRVRLVESFLPQS
                     KNDHVYEIVTPIYKKSSYRGPEIEIPKYKNTDIEYDKDWFALHIHIDKPSQDTFIIDNL
                     YPFVKHLKDKGDIDQYFLMRYIKQGDILKLRLFRNDENYAEIYSILKNWLPHVRQTTEV
                     SDYEFVSYEPEFFRYGGKNTINEIEAFFEYDTNLAVNIIENDFKFDRPYIVAISIMYLF
                     EMFSISNEERMEIVNNYVPTSFKSKDIRPFKNELVTICNPANNFEYMAKHYSGIYRILK
                     DGNQILSKLNEGLKKTLTTKRSRIIGSLIHMRCNRIFGIDKDQETFVLSIVKEIVKTQK
                     HWCGDKND"
     aSDomain        complement(13521..13799)
                     /aSDomain="Lanthipeptide_LanB_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing LanB protein in a class I
                     lanthipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_KSF82_RS09470_Lanthipeptide_LanB_RRE.
                     1"
                     /evalue="2.40E-15"
                     /identifier="RREFam001.1"
                     /label="KSF82_RS09470"
                     /locus_tag="KSF82_RS09470"
                     /protein_end="237"
                     /protein_start="144"
                     /score="48.0"
                     /tool="antismash"
                     /translation="VIWNSKAHIINDRIYLNEQSAIYLNNNKDTSFSIKNSELLVFIKT
                     TVTNNNITFSNLAEKINQEFEINDITKVKVYIHNLVSKEIIYSTIRPP"
     gene            complement(14296..14439)
                     /locus_tag="KSF82_RS09475"
                     /old_locus_tag="KSF82_09465"
     CDS             complement(14296..14439)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_b"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /locus_tag="KSF82_RS09475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09465"
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="WP_220668385.1"
                     /sec_met_domain="TIGR03731 (E-value: 2.2e-24, bitscore:
                     82.6, seeds: 23, tool: rule-based-clusters)"
                     /sec_met_domain="mature_b (E-value: 4.8e-12, bitscore:
                     42.9, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKVLDLDVQVKENNNSNDSAGDERITSHSLCTPGCAKTGSFNSF
                     CC"
     CDS_motif       complement(14368..14439)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEKVLDLDVQVKENNNSNDSAGDE"
                     /locus_tag="KSF82_RS09475_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(14296..14367)
                     /prepeptide="core"
                     /locus_tag="KSF82_RS09475_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="2.20"
                     /molecular_weight="2248.6"
                     /monoisotopic_mass="2247.1"
                     /core_sequence="RITSHSLCTPGCAKTGSFNSFCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEKVLDLDVQVKENNNSNDSAGDE"
                     /leader_location="[14367:14439](-)"
                     /alternative_weights="2266.6"
                     /alternative_weights="2284.7"
                     /alternative_weights="2302.7"
     gene            complement(15176..15319)
                     /locus_tag="KSF82_RS09480"
                     /old_locus_tag="KSF82_09470"
     CDS             complement(15176..15319)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_b"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /locus_tag="KSF82_RS09480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09470"
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="WP_000416759.1"
                     /sec_met_domain="TIGR03731 (E-value: 1.6e-20, bitscore:
                     70.2, seeds: 23, tool: rule-based-clusters)"
                     /sec_met_domain="mature_b (E-value: 2.2e-07, bitscore:
                     28.3, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNSF
                     CC"
     CDS_motif       complement(15248..15319)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEKVLDLDVQVKGNNNTNDSAGDE"
                     /locus_tag="KSF82_RS09480_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(15176..15247)
                     /prepeptide="core"
                     /locus_tag="KSF82_RS09480_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="0.40"
                     /molecular_weight="2420.8"
                     /monoisotopic_mass="2419.1"
                     /core_sequence="RITSHLFCSFGCEKTGSFNSFCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEKVLDLDVQVKGNNNTNDSAGDE"
                     /leader_location="[15247:15319](-)"
                     /alternative_weights="2438.8"
                     /alternative_weights="2456.9"
     misc_feature    15418..15435
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.94"
                     /tool="antismash"
     gene            complement(15561..16544)
                     /gene="lukD"
                     /locus_tag="KSF82_RS09485"
                     /old_locus_tag="KSF82_09475"
     CDS             complement(15561..16544)
                     /codon_start=1
                     /gene="lukD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /locus_tag="KSF82_RS09485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09475"
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="WP_000782464.1"
                     /transl_table=11
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITLY
                     KTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQFYW
                     GGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNGSKS
                     FSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNELFLGG
                     RQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMDRYTNQ
                     WNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            complement(16546..17481)
                     /gene="lukE"
                     /locus_tag="KSF82_RS09490"
                     /old_locus_tag="KSF82_09480"
     CDS             complement(16546..17481)
                     /codon_start=1
                     /gene="lukE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /locus_tag="KSF82_RS09490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09480"
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="WP_000473596.1"
                     /transl_table=11
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKRT
                     EDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRMIW
                     PFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFNYSK
                     TISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGSAREY
                     FAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRTGIYAE
                     RKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            18281..18820
                     /locus_tag="KSF82_RS09495"
                     /old_locus_tag="KSF82_09485"
     CDS             18281..18820
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500455.1"
                     /locus_tag="KSF82_RS09495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09485"
                     /product="hypothetical protein"
                     /protein_id="WP_001790220.1"
                     /transl_table=11
                     /translation="MVVYASHLSFLLFSNKKVLKINAYTGTDMAKLDLNSLDDEHVKLL
                     INELKYPETHIDVNELKTIVASRINERQEIISFKLGIKYLLTIKRGNIEKDRFSISIIF
                     KDTYHTLVRIDINGGTHDNPDGTIAPKSHIHIYNDKYDKKDRFAYEINLKDFPDIYNLY
                     NVYMSFLEYNNIKDLE"
     gene            18833..>19528
                     /locus_tag="KSF82_RS09500"
                     /old_locus_tag="KSF82_09490"
                     /pseudo=""
     CDS             18833..>19528
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /locus_tag="KSF82_RS09500"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09490"
                     /product="DUF1828 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTTFDAKKLKKEYLDWYNQTLEFSNLSNNVVRIDTPFKDNSLDNL
                     IIYALYDQSRDMITLTDDGYTIFDLENNGISLNKSKKRKKIFEEHLSAYGIKYNDKTHE
                     IFVQTNFKNFNKSKHNLLQCLIFVNDMYLLSNPKSQNIFTEDVANKLDEHNIYYGRDLP
                     IIGSSGVVHNFDFFISAKKNQKEKFINAISNPNNSMIIKSKITDAMQAKKIKRHRQNEF
                     IFILNDSKKD"
     gene            19627..20799
                     /locus_tag="KSF82_RS09505"
                     /old_locus_tag="KSF82_09495"
     CDS             19627..20799
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1130: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /locus_tag="KSF82_RS09505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09495"
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="WP_000195429.1"
                     /transl_table=11
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTEY
                     IQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKALM
                     ASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPY
                     LMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKER
                     GLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGI
                     FKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLI
                     ERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            <20846..20953
                     /locus_tag="KSF82_RS09510"
                     /old_locus_tag="KSF82_09500"
                     /pseudo=""
     CDS             <20846..20953
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /locus_tag="KSF82_RS09510"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09500"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="DSKKEINEHNKNLLHENYISTIDYSELDEKIGLLI"
     gene            <21414..21622
                     /locus_tag="KSF82_RS09515"
                     /old_locus_tag="KSF82_09505"
                     /pseudo=""
     CDS             <21414..21622
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /locus_tag="KSF82_RS09515"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="KSF82_09505"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="NLDFNNITNDAYIYRDIKPNSPSTNKLEYIMLIIVNDISSKDRKL
                     "
     tRNA            complement(22196..22284)
                     /anticodon=(pos:complement(1956846..1956848),aa:Ser,
                     seq:gga)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KSF82_RS09520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KSF82_09510"
                     /product="tRNA-Ser"
     gene            complement(22196..22284)
                     /locus_tag="KSF82_RS09520"
                     /old_locus_tag="KSF82_09510"
     tRNA            complement(22339..22410)
                     /anticodon=(pos:complement(1956974..1956976),aa:Glu,
                     seq:ttc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KSF82_RS09525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KSF82_09515"
                     /product="tRNA-Glu"
     gene            complement(22339..22410)
                     /locus_tag="KSF82_RS09525"
                     /old_locus_tag="KSF82_09515"
     tRNA            complement(22412..22486)
                     /anticodon=(pos:complement(1957051..1957053),aa:Asn,
                     seq:gtt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KSF82_RS09530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KSF82_09520"
                     /product="tRNA-Asn"
     gene            complement(22412..22486)
                     /locus_tag="KSF82_RS09530"
                     /old_locus_tag="KSF82_09520"
     tRNA            complement(22501..22574)
                     /anticodon=(pos:complement(1957139..1957141),aa:Gly,
                     seq:tcc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KSF82_RS09535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KSF82_09525"
                     /product="tRNA-Gly"
     gene            complement(22501..22574)
                     /locus_tag="KSF82_RS09535"
                     /old_locus_tag="KSF82_09525"
     tRNA            complement(22590..22665)
                     /anticodon=(pos:complement(1957229..1957231),aa:His,
                     seq:gtg)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KSF82_RS09540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KSF82_09530"
                     /product="tRNA-His"
     gene            complement(22590..22665)
                     /locus_tag="KSF82_RS09540"
                     /old_locus_tag="KSF82_09530"
     tRNA            complement(22679..22751)
                     /anticodon=(pos:complement(1957315..1957317),aa:Phe,
                     seq:gaa)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KSF82_RS09545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KSF82_09535"
                     /product="tRNA-Phe"
     gene            complement(22679..22751)
                     /locus_tag="KSF82_RS09545"
                     /old_locus_tag="KSF82_09535"
     tRNA            complement(22768..22843)
                     /anticodon=(pos:complement(1957407..1957409),aa:Asp,
                     seq:gtc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KSF82_RS09550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KSF82_09540"
                     /product="tRNA-Asp"
     gene            complement(22768..22843)
                     /locus_tag="KSF82_RS09550"
                     /old_locus_tag="KSF82_09540"
     tRNA            complement(22865..22938)
                     /anticodon=(pos:complement(1957501..1957503),aa:Met,
                     seq:cat)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KSF82_RS09555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KSF82_09545"
                     /product="tRNA-Met"
     gene            complement(22865..22938)
                     /locus_tag="KSF82_RS09555"
                     /old_locus_tag="KSF82_09545"
     gene            complement(23410..23964)
                     /locus_tag="KSF82_RS09560"
                     /old_locus_tag="KSF82_09550"
     CDS             complement(23410..23964)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /locus_tag="KSF82_RS09560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_09550"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_000132890.1"
                     /transl_table=11
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAKT
                     VDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAENID
                     LDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGGHFL
                     EEDGFETFTALQDRMQDYMTR"
ORIGIN
        1 cagctgaacc tgagttgcct ggttctacga aagcatcagt aataatcatg ttgcctttaa
       61 ctgacagcac ttttcctgtt gactcataca tttgaaattt atttatatat ggttctggat
      121 agccaacaat tgaaatgcgt tcattttctt tagcttctga tgctattttt aaaatgcctg
      181 tgtaatcttt aaaattcctg ttttttggat gaacagcttt atcttccaca tgtagaatgg
      241 caatatcttc ttgacctgaa taacggacaa tttttttaac tttataaaat ccgccattat
      301 tatattcacc attgggatgc gctataatat gtgcaccaat ttccatacct gcaacgacat
      361 gcttgttggt aacaattgta tttttaccga caatgaaacc tgttccagat cctatcgaaa
      421 cgacaccatt gtatggtgct acattagtat ttttgattag tttcacatta tgttcagctt
      481 tagccgtttg ttgaataccc tcaaccactg ttgtgccgac gccagtcact gatgttaaaa
      541 tcgtcaatgc tgcaatactt ttgatgatta tatttttatt catttttgtg ctcctctaag
      601 tattcgtata aatataaggt taggtttttg aagttttatt ttttaaatca tttcgaataa
      661 atagaatcac agagatgttt aaaattgttg attttgttgt taatttctaa aaatcattta
      721 tcaatgtatg gatggatgat ttatttatct aaattatctg caataaattt cttaatttca
      781 ggagagaaat aaacagcaaa tgaccttgta ctttcacctg ttggtttatc actagcatac
      841 ataacaccaa ttgcttctcg cttactattt aatataggtg aaccagagct gccaggttgg
      901 acaaccgcat cagatgtcac tatatttcca ttcactgata gtactttacc agttgattca
      961 tacatttgta gtttatttcc attaggattt ggataaccaa tgactgatat aggttcattt
     1021 tctttagctt ctgatgctat attaaatttg ctagtgaaat ctttgaattt tctacctttt
     1081 ggttgcgttg atttttcttc aacttgtacg accgcaatat cttctttacc aggataatct
     1141 acaatcttag taactttata aagtccacca ccgttattat aaaaaccatt aggatgtgct
     1201 ttgatttcat caccgacttt catgtgataa gtaacatgtt tattggtaat gattgtatga
     1261 ttcccaacta caaatcctgt tccagcgccc atccatgtaa caccactgta tggtgcaaca
     1321 ttcgtgttgg taattaattt cacactattt tctgctttgg ctgtttgttg aataccatca
     1381 accactgttg tgccgacacc agttattgat gttaaaatcg tcaatgccgc aatacttttg
     1441 atgattatat ttttattcat ttttgtgctc ctctgtttat tcatgatgct tttgatatag
     1501 acgttttttt atgttatatt cgaccgatgt ttttcattaa aattattgaa cacatgctca
     1561 tggtgtatat ttaaatttgt ttattgttca atgtgctttt gaataaaatc tttgatttga
     1621 ggcgtaaagt atacggcacc attatattca gaaccaattt ttccaatacc gccatacacc
     1681 acacctatga cctcattgtt agaatttaga actggtgatc ctgaattccc gggttcaatg
     1741 tatgcatcaa aatttaaaat attgtctttg attcttttta tagttcctgt agattcaaac
     1801 tgtttaaaac tattttgagc aggtaatggg taaccaataa ctttaatttt gtcatcaact
     1861 ttagcatctt tcgcaaaatt gaatgcttgg acattttcat taaaaataaa gccttttggt
     1921 ccacgttcga ctgcttgttc ttcaatattc atgacagaga tgtcttcatc acccggataa
     1981 tcagaaatgc ttttaatttt atatatacca ccatttcctt tgtcaccgtt tggatgggca
     2041 gtaattctat cgccaacttt ataatctttt gatacatgtt tattggtgat aattgtattt
     2101 tttccaatta caaaacctgt cgcatcttta aatgaaacga cgccattata tggaaaaata
     2161 tttgtatctt taacttgcgt aacattcttc tctgcatttg ctatttgttg tgtctcttca
     2221 acgactgcag catttattcc agttactgag gttagaatgg ctaatgctgc catgctttta
     2281 atgactatat ttttattcat tttcaagctc ctttacaatt cgagatatgt tgcaacgctc
     2341 gtttaaagtc aatttgttta tttatctatg ttttctgcaa tgaatttttt aatttctggt
     2401 gtaaagtaga cgccatatgc atttctgtta tcatcatttt ttacatcaga agcaaaatga
     2461 ataccaacta attcgttgtt gctgtttaat acaggtgatc cagagtttcc gctttcagta
     2521 tgcgctgaat atacaatact gctaccttct actgacatca cagggccagt tgactcatat
     2581 aaaacatatt tatttttgta tgggtgtgga taaccaatca ctttaattcg ctcaccagct
     2641 ttagcccctg ccgcatattt gaatggcgtt acattatcat taaaattaaa gccttttggt
     2701 ccacgttcta ttgcacgctc ttcaacttga atgactgata catcttcttt acctggataa
     2761 ttaataatct ttttaatcga ataaatacca ccattacctt tatcactatt tggatgtgca
     2821 gtaatacgat cgcccacttt gtaatttttc gacacatgtt tatttgttaa aatagtattc
     2881 tttccaacta caaatccagt tgcactttta aaagcaacta caccagtata tggaaaaata
     2941 ttagtatctt taacttttgt gacattattt tctgctttgg cagtttgttg tacttcctca
     3001 accaatgttg ttccaatacc tgttacagat gttaaaattg ttaatgctgc taaactcttg
     3061 atgactacgt ttttgttcat tttaagttcc tctcttttaa ttattttgat atctagatac
     3121 ataattcagt atatttagtt aattatgtaa ccgttttctt aatcactctt taaaaaatca
     3181 acttcacgaa tgaattgatg atacttattt ttcaatatta ttttgaataa attcttttaa
     3241 ttgtggtgtg aaataaacac cgaaattctt ttcagattca tcttttccac tacctgcata
     3301 taaaatacca atcagttcat gtttagaatt caatacagga gatcctgaat tacctggttg
     3361 tgcatacgca tcaaattcca taaacgttcc actgatatgg ttaatcgttc ctgtcgattc
     3421 aaacatttta tattttgttt gtgcaccctt tggataacca ataacagaaa ttctatcttt
     3481 cacttttgct ccgtctgcaa attttgtata actaacgttc ttattaaaat tcaaaccttc
     3541 tgtacttgtt tcatgaacat gaactatcgc aaggtcttct tttccgggat attctacaat
     3601 gtctttaacg tcgtagtttc ctccgccttt acctttactc gaatgatgtg ctgatactct
     3661 atttttaaaa atatcattac ttttagcgat atgtttgtta gttacgattg tatttttacc
     3721 aacaactaca ccagtaccac ccacaaatgc taccactgaa ttgtatggtt ccttagttgc
     3781 atcggtaatt tctttgacat tcttttctgc tttggcaatt gaatgaggct gattagaaat
     3841 attttcagca aaaccaagag atgttaaaat agttaaagca gttaaacctt taaccattac
     3901 atttttattc atattttacc tccttccaaa tttattttta tagttaatgt atctagatac
     3961 acatttacta tatacatttt aaataaaaat attaattaaa tattaattaa caacttatta
     4021 acgttttatt aaattatttt acatgaattg actcatttat ttaaatatgc ttaaaactca
     4081 atatcacgca attaaaattg actttttagg attattttgg tcttaaatat taattattga
     4141 attaaaattg taatatttat tcggaaatta ggtgattgaa aatatatggc ggaatggtga
     4201 acaattcatg tatttataat ctttcacttt actatgtatt gtgtttaaca acattattag
     4261 cgattagttc ccctattatt tatgacaata tgcagtcaat caatgaaaca tgtatgtatg
     4321 agtatatttg gaactagtaa tgtattgtta aatattgaag atgattgaat tatggaattt
     4381 ttttcgagat atgttatttc ggggatcaga ttttgcgatt ttgccttgga tagagtagtt
     4441 gcgatttgta aatacgtttt tcaggttcga ttcaatttcg ttgttaagat aatatggatt
     4501 agtttcactg acatgttgat gcatgtcttt tttgttctga taaagtataa aatgtttact
     4561 taacattact tagtatgaca cctgctaatt caaacattat ttgagacatt cttttcaaat
     4621 taattataaa tttttaccta tagactagtt tgatatttat ctacatctca aaattctcat
     4681 caacaatctt tcacatccaa catttttact ttagttttta taattcaaaa caacaaaact
     4741 atgttaaaaa attattctat tttttagtta atagatagtt aatacatttt tgatatttag
     4801 ttaattgttc ttttaaaaaa ttattattat attttaattg taaacgttta caatataaaa
     4861 aaaggagcaa taaaaatgaa tacaaaattt ttaggtaaaa cattagtagc aagtgcttta
     4921 gtattaacaa cattaggaac aggcttacat tcttcatact taggattaga tacaaataaa
     4981 gttgttaaaa cggctaaagc agaagaaaaa atgacagatg gtcaattatg gaaaaaagtt
     5041 aaagattcat tacatgattc tgatattatt ttaagtaacg agtacgaaac aatcaacgtt
     5101 acttatttat taagcaatgg atattctagc agcgtttctg ctcctggaaa tgatgatgga
     5161 ggccatctaa cacaatctat agactttaaa ggattaaaac aaattgattt aactaaggaa
     5221 aatgtctacg atgactttaa caaaaagcta gatgctaaaa atacatggaa ttctctaacc
     5281 gagaagttaa aaggtcttgg tttactacaa aatggccaaa aagtatctat ttattcttca
     5341 gatagctctt ctccagtctc gggtaaagtg ggagaaggag taactagtgg tggcgaaaat
     5401 actctaacta aacgcttcat caacaaaatc acaattgatt aaaccttaaa ctcaaatttc
     5461 cttaattcat aaaacattaa taactaaccc tttcttacaa accacctagc ccataatata
     5521 ttaaaacgtc atttttattt caattagcgg aaaataaaaa aaccattccg cattaacttt
     5581 ggcaagttgg aatggttaaa aaaatatttt agaagccagc gtttggcttg cttagacgac
     5641 atgttcgcgc atgtcgtctt atttttacat ttatataata acacgcaatt caataaaatg
     5701 tctaatcaat aaacctcaat tacattcaaa ttcaaaaact tttcacctaa tattcatcgc
     5761 ttaaataaac attacgttta attaaacaga cattgtcaat cgcgtctaat acccctgctg
     5821 tccgaaaaca aaattatttt gtattcatat ttaactgttc tctttcctcc cttttcaacg
     5881 caactaaatt tcttttaccg ttagagcccc aatctttaat ctttaattgt tctaccgctc
     5941 catttattaa atcctttaaa gagtaaaact gctaatagca acgtgataat aatatagatt
     6001 gccaatgtta atgtaactgg tatactccct tcgataaaca tataaacgta acgtgtagca
     6061 tatgtgattg gtaaatagaa ccacgaatga tctccaagca cttctaatcc aaaataaacg
     6121 ttaaaaataa acattaaaac tccgacaaca atagccatta catctttaat gaaaatacta
     6181 aaaataaaaa gtagcagtaa tataattaca ttgaaaaaca atgatacgcc tataaacata
     6241 agtgttattt tcatatcatg tgaatgccac aataaattaa ttgatgctaa tagaatacat
     6301 atggctgtaa tagtataagt aaaaatcatt gatgcattta accaatttag cctattagct
     6361 tttcctaaaa tatgattaaa gtgaccaata ttttcttcaa aattgataac ttgatagacg
     6421 tttatagaaa ttaatagcga tgtaattgca ttaaaactcg ctgtaaacaa acttatttgt
     6481 cgaccattcc ataaatttac gtttaaatac caatttataa ataatataaa caatatggtt
     6541 acaataatgg gtacaaatgt gagcgcttgc ttagaaaatt tcaacttaca tgactttaat
     6601 tcgttaatta tcattttaat cgccaacttt tcttattatt taaaaatgtt actatcgcga
     6661 aaataatgat gctaacaata atcgaaatat ataaggcatt gagatcatta aagtattttg
     6721 aattatggtt tacaactaaa ccattaggta gtataccaag cgtaataagc gggattctct
     6781 gcattatccc ccacggcaac acccaaaata aactcttcaa tgttaaaaac aagacacaaa
     6841 tgactgataa tactaagttt attaatattg atacgaacac accaaagtat cgagttaata
     6901 aaaagttgag cggtatcaat ggtagagata ctacatacat caacaatatt gtcaccaata
     6961 acaacatagc attaaccgga tgtggattaa taattaggtc acctatataa gcaataataa
     7021 atactaaaaa gcaatgtacc aaaaatgcta ttgataaaat gaaaatcttt gctcttattt
     7081 cttttgtaat cgaccaatta ttacttaagt aataattaaa tgatttattt ctcatttcaa
     7141 ttttaaataa cgaattacaa gccatacata atacaatcgg gatgaaagca attggccaaa
     7201 tattaaatag taaagttata tatggtgaca cactattcgc tgttcccgta ttacttttgg
     7261 cgaataagac tgtgaaaata gcaaaacaaa gaaataccag cggacttatt aataaaacat
     7321 acatactgaa agaattttta aatttgatac cttctatctt taaatgtatc atttgtaatc
     7381 acctttcgtt attttgaaga atatctcttc taagttttca tctttgttat ttctttgctg
     7441 atattttagc tcaccctcat gaataatacc gatatgatct gctaaaactt ggatttcaga
     7501 taaaatgtga cttgaaataa taatactagt accttgttct gttaataatt ttagaagttc
     7561 tctaagttct tggattccat atggatctaa accattagat ggttcgtcta atactaaaat
     7621 ttctggcttt ttaattaatg ccattgcaat tccaagtctt tgtttcatac ctaaagaaaa
     7681 gtccttaacc tttttctttt tatcgacatt taaattgact aagcttaaaa cactattaat
     7741 ttcgctgaaa tcaacgcttt catttaaaca cacaattttc aaattatctt gtgcacttaa
     7801 atgattatat gtcgcaggtg tttcaataag cgaaccaact ttgtgcaaat catttcgtgt
     7861 cattggtcta ttatctaatt taatttcccc tgaatcttgt tgaagtatgc cacatataat
     7921 tttcataagt gtcgttttac cggctccatt aataccaagt aatccataaa cttcgttatt
     7981 ttctaaagtg atattaatat gctttaatac atcttgattt ttaaaccgct tcgaaatgtt
     8041 ttcagtaact aattttgcca taatcaaaaa ctcctttgat ttattaactt gctttttgat
     8101 ttgctacatt taatgcttta tacacatcaa gctcaccatg cccatatcta ctaaatggtt
     8161 tattattctt agatgtccca tgctgatata acaattcaat cgctttatct ggatgttttt
     8221 caagatgata tttatcaatg attaaagcta gtgctcccga aactttaggt gtggctaatg
     8281 aagttccagc ttgataaata tatcttccgt tattggcagt agttaaaatg ttctccttat
     8341 gcatataccc ttcattcatc catttatcca caccgaattg atttaaataa gcaaatgatc
     8401 ctccgggcgc agcaatatct gtataattca taccaaaatt ggaaaactca gatagattac
     8461 tcttttgatc tgtagatcct actgtaacga cattgtccat agatgcagga acatctttca
     8521 cttcgccatt accttgatat tcacgctgta attttagttt ctgtttgtca ttgacatcaa
     8581 taccatcatt accagctgca gcaacaacga tagatttttt cttcttggcg taattgattg
     8641 ctttctgtaa cgcatcgtat tctacttttt catcttttct aaatgtttga tggtcatttt
     8701 tgtccaaaat aatataacta ccaacactaa tattaatgac ttgatttcca tcatttgcag
     8761 cttgaacaat cgcttttgat acccaaagca gttctgtttt tttactacca aacacgcgat
     8821 acattgtaaa tttgttattc ggtgcaacac ctattaactt accattagca ctcgtttgac
     8881 ccgacaccat cgtgccatgt cctttcctat cattgacatc gtgaacatca cctgtttcct
     8941 ccggttcagt acctctaaaa ccgtttaaag gtactaaatt tttagaatca gtcgagaaat
     9001 tatttttcaa atcgtcatgg tttttcatca cacctgtatc tatgattgct attttggtgt
     9061 tagcatgttt tggcaaatca tcatacgatg caccattatt ggttatttta ttcatatccc
     9121 attgtcttga aaataatgac tcatttgatg ttctgtctat tgttttctcg ctagtaatac
     9181 aagttgaaca tgtggcattg atatatttaa tatcattttt atagtttgct aaagcattag
     9241 catgcatttt cgtcatttta atctgtgcca catgtaattc cggtatatta tagacaacgt
     9301 ttaaggactt ctttttaact aacttgttaa atgttgctgt atttttgtat tcaacactgt
     9361 aatatagttc ttctgacgct gatgcattac tcattgtaat agatattaaa agactcaata
     9421 ttattaaact aataatagcg cgctttatga ttttcatatt ctaatctcaa atgaattcca
     9481 gaactttata tggttcaggt gcgacaacat tctttttaaa tgtttttgac gctaattcat
     9541 aactttctga aatatttgct ggatatattg atacaccata atcttttaat aatcgaatat
     9601 tattttgagt aactggattt tcccacattc gtaaattcat atttggaaat atagaaagtt
     9661 tttcaaaagc tgtatgacaa atagttaata ataaattgtc acatatacca tttgcaattt
     9721 tattaatcgt attagaagtc gcaggtaaaa taataatctt gtcatgttta tttgctatat
     9781 ctacatgatt taaaaaaggg tcttcaaatt cgtcataata gttatcgcaa aattgcttta
     9841 atatttcacc attaataaac tttctaccgt ttgttgacgc gattacattt acttcatcaa
     9901 attttgattt taattcaata atatagtggc taatattgat actattaact gagccacaca
     9961 aacaaatcaa tacattttct cccatgatat acgcctccat tcagctaatt aaaaacgcat
    10021 tggtccaatt tttttcattg agaatttttg tatttttcag tgtcatcaat gttagataga
    10081 cccctaattc accttctaaa aggccaactt tcctactttt aaatacatct tgcttatttt
    10141 cattaatgtc tataaaattc accagataat cttccttata atgagaaatc agtttattta
    10201 ttaatacagt gatataatca gacacttcat ttaattcaaa attcaaattc ataattgtta
    10261 acattaatat ttgtgatgac aatccatgac aaatagttgg acttattaaa ttttcatctt
    10321 tatcatttac tacttgtagc atcacttttt tagacatttt aattatttca tcatcttgta
    10381 atgcttggcc gattaaaaac aacgtcgtca ttacacccgt attgccataa caccaaccat
    10441 tccgaataaa attgaaatga ttacgttcag aaattaaatc gtaacgctgc aaccatcttt
    10501 cgtggttaca aaatttttcg tccatgataa atttgtacat gtcttttaat atgtgctgat
    10561 gattttcaat cgtaattcct ttaatcacgc ataatgcaaa taaagacatc ggtcctagca
    10621 ctccatgtgc aaggccaaga ttgatattac cttcagtaaa ataattttta tcagactcta
    10681 aaaattgact ttcttgtggg actacccagc tatttttaga atattgaata tctttaaagt
    10741 atactaatat gctttttaat gctttaacat taaattcatt ctcatctact ctatttagca
    10801 aatatctccc tatcccagct aatccactga cagtatcata atttaatgga tttaatggtt
    10861 cttgtaatat ttgatcaaat ttattttcta tttcatttac aatgtgacca tcaatattat
    10921 ttaaaatatt ttgataattt ttgccatttt gcgatgctaa atccattgaa aatgcaatac
    10981 ctgaaagtcc accaaacaaa gaattgttat attgaccact ctgtaaatat ggtgctaatt
    11041 tcatgatgta cttatgcaca atttgctcag tattaatatc gaaaactttt tgataagcat
    11101 ctaaaaacaa aatgatacct ggtataccat gtgataaagt tgaaggttca aaataatcgg
    11161 tttcagtaga tgcttttgaa atgaaatcat ctacttctga aatctcttga aacttcttct
    11221 tcaatattag attcatatca atcatttttg tcaccacacc aatgcttttg agttttaaca
    11281 atttctttta caatagataa aacaaatgtt tcttgatcct tatcaatgcc aaatattcga
    11341 ttgcagcgca tatgaattaa gctgccaata atccttgacc ttttagtagt taaagttttt
    11401 ttaagccctt catttaattt gctcaaaatt tggttgccat cttttaaaat tcgataaata
    11461 ccactatagt gtttagccat atattcaaaa ttattagcag gattacatat agttacaagt
    11521 tcatttttaa aaggtctaat atctttactt ttaaatgatg taggtacata gttattaaca
    11581 atttccatac gctcttcatt agaaattgaa aacatctcaa ataaatacat tattgaaata
    11641 gcaacaatat atggtcgatc aaacttaaaa tcattttcaa taatattgac agctaaattc
    11701 gtgtcatatt caaagaaggc ttctatttca ttaatcgtgt tctttccacc atatctaaaa
    11761 aattctggct cgtatgatac aaattcataa tctgaaactt ctgtcgtttg tcgaacatgt
    11821 ggtaaccaat tcttcaagat gctgtatatt tctgcgtaat tttcgtcatt cctgaataac
    11881 ctcaacttta aaatgtcacc ttgtttgata taacgcatta aaaaatattg atctatatct
    11941 cccttatctt taagatgttt tacaaatgga tacaaattgt cgatgataaa tgtatcttgc
    12001 gatggcttat cgatatgaat atgtaatgca aaccagtctt tgtcatattc aatatctgta
    12061 tttttatatt ttggaatttc gatttcgggt cctcgataag atgatttttt ataaattggt
    12121 gtaacaattt catacacatg atcattcttt gattgaggta aaaatgattc tactaaacgt
    12181 acacgtttat gcttcttata ttctttcatc aataaatatc tatgattagc taatgataaa
    12241 tttagtagaa gcttgttatc tccataaact aaattcacga tattaggtat attgaattgt
    12301 tcttgatatt tcaaaaattg ttgatcccat tcttcatttt taggtaaagg taataccatc
    12361 tcgttaattt tccaatatgc tggtttaaga atgacatttt tatatctgat tcgtggtgaa
    12421 taactaaatg aatcaatact cacatcattc ataggttcaa tgcatttcac agattccatt
    12481 gaaatttctc gtaacaatcg atataaatta cattctttta aaaagttata catattgttt
    12541 gattcaaata gtaccctttt atttagttgg ctagaatata aatataattt gttaaaggta
    12601 gcaccaacat aaatatcatc taaggtaagc tcatactttg aataactatt actacttcct
    12661 aaattcaatg aatattcgta tgagtcatga ttagttaata cattatgatt tctcggatat
    12721 ttcggtatgt tatttataga aatcatttct acattgtcat cacatatcat cttctgataa
    12781 taatgtcctt tttcatgttc taattttgct aaagtttcag tatcaattaa atgatgaaat
    12841 cgcccaaatg tagcgccagc attaaaagac gccgttaaag gactaataac aatgagctca
    12901 ttgtactgat tgtaaaaacg tcctaaataa agttcagcat aaacatctgc actcattggc
    12961 gcatgataat gattgatttc attatcgtta atcaatgact ctacatcttt ttcatttatg
    13021 actatctcat cgttatttct caaggcatgt aaaaattttt gcttaagcat tacaatatta
    13081 tttccatcgg tttcttcttc ttgaaaaatt gttgtcccga aacctgttat atctgaaatc
    13141 aaatctttaa tattcacgag ttgttcaaat ccatactttt caataaaacg gttgtgaaga
    13201 actttcaaat ctgtgaatcc aatattattt cttgataaaa gccacaataa gtacgctgct
    13261 tcagatatat ttgttgctat atcttgatgt aaataattgt taatcatgtc aattttggta
    13321 tcaatttgaa gatagttttt acatttgaat agtgccttca tatgatgaat aatgtcttta
    13381 tataattcct cgccaaatcc aatttcagtt ttttcataag ctaatatcaa tttctggatt
    13441 tctctaattt ttttcacaaa atcatcatta tgtaaagaaa gtttatttaa aatataattt
    13501 aaattatcgc tatatgataa cggtggtctg attgttgaat atataatttc ctttgagaca
    13561 agattatgga tatatacttt cactttagta atatcattaa tttcaaattc ttgatttatc
    13621 ttttcagcta aatttgaaaa agtaatatta ttattcgtca cagtggtttt gatgaatacg
    13681 agtaattcgc tatttttaat ggaaaaagat gtatctttat tgttgtttaa ataaatagca
    13741 ctttgttcat ttaaatatat tcgatcattg ataatatgtg ctttactatt ccaaataact
    13801 ttcaaatttt gataatattc atcaatactc tcaatataac taaccagttt atacaaccat
    13861 tcgccatcga ctttcacata tttttgaata ctatttccaa ctttaagacg tgttggttca
    13921 tttacaaaat gccccaaatt aataccactt aataatccaa atggagttgg acgtgttgac
    13981 attctaatta aatattttaa taaactttct ttagcatctc taaccttctt gttatcgcca
    14041 tcaaaattta tttcttgtaa agtacaatat aagttaaatg ttgttgttag gattgattct
    14101 ttcatctgag catttaattg taagtcgcta tatttaattt gttcagtatt taaaaataaa
    14161 ttgaaaaatt caactgataa taaaggtgtt ctaatcatac aaatacttga cggttcgtaa
    14221 atagtgttca tttgtaagca cctcacatat ttgaatagac aaatagagcc ctttcaggct
    14281 ctatttgctg taatcttaac agcagaagct attaaaacta ccagtcttag cacaaccagg
    14341 agtacataaa ctatgacttg taatacgttc gtcacctgct gaatcatttg agttattgtt
    14401 ttctttaact tgcacgtcta aatcaagaac tttttccata gataaaacct cctattatta
    14461 gttgaagtta aggcctactt caattgtcat attatccctt ttcacacaac aaatcaataa
    14521 attatataat tatattttgt gaattttttg taaacactta ttaatttagt aatactattt
    14581 tgataattag atttttgtgt ttttttatta ctatttattt tcactaataa ttttgcatct
    14641 aagttcataa ttttaaattt attgatgaat tttttaatag attaatcaat tcatttatac
    14701 aaatacttag atatttttat ctatcaatac tattacgaat ttggagatgg ctgcttgaga
    14761 ttaattaata gatatttatg cagttttgtt ctatttatac aaaatttagc aaataataat
    14821 tgaagttatt ttaaagatga ggatataaac atataatttc aatgacttga ttattttctt
    14881 aaatgacatg ttctaagagg gtcaatataa taaccaaagc ttgtcattac ttatatgtaa
    14941 agatagaggc taattacata atcctaactt atttaacagg aagtcatgaa aactatatta
    15001 caactaaaag tataactttc catcaccaaa gtcagaattt attgaaacac tcaactgata
    15061 atatagaagg ctaattataa aaatacctgg cgattaataa actgtttccg ttttttagca
    15121 cttaatatat ttaaaaagac aaatagagcc ctttcaggct ctatttgctg tatccttaac
    15181 aacagaagct gttaaaacta cccgtctttt cacaaccaaa gctacaaaaa agatggctag
    15241 ttattctttc gtcacccgct gaatcattag tgttgttatt tcctttaact tgcacgtcta
    15301 aatcaagaac tttttccaaa gataaaaccc cctatttaaa agttgaagtt aagacccact
    15361 tcaattgtca tattaatcat taccacacaa taaatcaatg aatttaataa taattatttg
    15421 ttattaattt gtaaatcgtc attaatttta tggtactatt gttagtttaa acaaataatg
    15481 taacttcttt ttttaatatg ttgtgaatat tttttgttta actcttaaat ttcgggttta
    15541 acttcctcta tttctaacag ttatactcca ggattagttt ctttagaatc cgtaccgatt
    15601 aatttaacag tatggttttg ccagtcaact tcataagtag atgtaaacgt tactgtattt
    15661 tgatttttgt agttattacc aacccagtgt agtcgattcc attgattagt atatctatcc
    15721 atttctcttt ggtaagttac tttgatttta gatttttttg tatcattttg tttatgagaa
    15781 agtacgctta taaattctgg gttaaagtta ccacgcgcca ataaaggcat ttgatgtgtt
    15841 ggcaagaaat tttgaccagc atttgaacta ctttgtctac cgcctaaaaa cagttcatta
    15901 ccatatgttg ggtcataact atctctacca tatggtcccc aaccattatt cataattttg
    15961 tgcgcctcaa caccccagcc aattgattta tgatttgttt ttctatcaat cgtagttctg
    16021 taactttctt gtttataatt tatcgtttct gaaaatgatt ttgatccatt taatccacct
    16081 gataagccat tagatatatt aatatctccg ccataagaat aacctaatgt ttgttgaact
    16141 tggaattctt cattttgatt tttaggtgca tagtcaacaa catttacagc atcatttgat
    16201 tctgaactaa ccgaaacatt atacttaccg ccccaataaa actgtgagta attgtaatct
    16261 tttggattag gctttttata acctgaatta atgtttccgg ctgccttaag tactaacgta
    16321 tctttgtcat aacttttatc cttaatgaaa ttaaacgtta aaatttgaga aatattcaat
    16381 ttatcattat cagatgttgc tgttgttttg tataaagtga ttttgtcatc tacttttttc
    16441 tcgcttacag gtgtgatatg ttgagctgca tcaactgtat tcgatagcaa aagcagtgca
    16501 attgatgaag caactgatga tttgactaat tttttcattt tcatattaat tatgtccttt
    16561 cactttaatt tcgtgtgttt tccaattaac ttcatatcta actacaaagt ttctatttac
    16621 aaatgcatta tgctttcttt ctgcgtaaat accagttcta gggaataaag tcgcatatgt
    16681 aatatctaag tttctaccat atgaaatttc aaattcactc gtatcacttg aacctttttc
    16741 atgtgatagt gtagtgataa acgatggatt aaagccactt tgaactaaag gtggcaattg
    16801 attatcagga gcaaaatatt ctcttgctga acctgttgga ccatttggac tttgtacgaa
    16861 taaatatcta tcatgcgcag atttttttcc atcaggcgta acaaattcgt ttgctttaac
    16921 accccattta acagattttg agttttgctt gtctacttca ctgacataac tcttttgggt
    16981 ataactaatt gttttagaat aattaaatga gccattgcca cctatagatg gtgctgactg
    17041 gaaattacct ccaatgttat atcctaatgt ttgaccaaca tcagtagttt ctattttgtt
    17101 tttaggaagg taattgatta agctaacatt tggatcttta gtcgtcagtc ctatattata
    17161 ttggaatggc caaatcattc gtttagttaa ttcatatcca ctacccttca catctgaaaa
    17221 tgaagttctg gaattaataa aaccttgcat tttaacaatt aaagcgtctt tgttatattt
    17281 tttatctttt acaaagtcga attggacatt ttgagtaacg ccccatttct tactacttac
    17341 atcctccgta cgtttgatta cttcagcacc atcaccaata ttttcaatat tagtatttgc
    17401 tctagattct tgaatcggag atgctaaagg tgcaatcagt cctactgaca aagttgcagc
    17461 taacattttt ttcttaaaca taagtttcac tttctttcta tataattttt taatacaaat
    17521 ttattctaga ttatttcact tatttataaa attaactgtt cattaaaaaa taaaatgcta
    17581 taaaatatta aaaatgacac aatttattca tttatgctgc tgaaaaccgg tcattttggc
    17641 gcaaccttca ttacctttaa atagttccct ttatattcga acaatatgat aaaattaccg
    17701 atactcaatc ttttctctgt aaaaataaaa acgaacttta atcaaatctt caaaaataat
    17761 gtttgtatta tcacaaataa tctataattg aatttaaaat taactgatta ttaactattg
    17821 tccgttaaac gaagatgatt acttcgtata ctgttataac tgaaatgttc tatatatcat
    17881 ttcgtaatat cgcttacttc atctaaaaaa atttaatgct atgatttagt ctaattaaaa
    17941 acatatatat atttactatg aactaaaatt tcactttaaa aataaaaaac catgatttcg
    18001 aagtcttcat aaactgaagc ttctactctc atggcaattt atactatgat attcaattat
    18061 aatgcctagt ctcatagtaa acggctacta cattcgcatg tacttcgttt tactgtccac
    18121 gcatggtcgt tgacttattt atattataat ataaatattt tatttttcaa tacttaattt
    18181 taaaaatccc ctatgaaaat gttgatgagc tacatcttta aatcttatta tacattgtat
    18241 aaaattatat tgcgaggtag taaattgata ttatacactt ttggttgtgt atgcatctca
    18301 cctatctttt ttgctttttt caaataagaa agtattaaaa attaatgcct atacaggaac
    18361 agacatggct aaacttgatt taaatagtct tgacgatgag cacgtaaaat tattaataaa
    18421 tgaattaaaa tatccagaaa ctcatatcga tgtaaatgaa ttaaaaacaa tagttgctag
    18481 tcgaataaat gaaaggcaag aaataataag ttttaagtta ggaataaagt acttattaac
    18541 aataaaaaga gggaacatag aaaaagatag gttttcaatt tcaatcattt tcaaagatac
    18601 ctatcacacc ctagttagaa tagatattaa cggtggtact cacgataatc cagatggaac
    18661 aatcgctccg aaaagtcata ttcacatata taatgataag tatgataaaa aggataggtt
    18721 tgcttatgaa attaacttga aagatttccc cgacatctat aacttgtata atgtgtatat
    18781 gtcgttttta gagtataata atataaaaga ccttgaataa aggagggata atatgactac
    18841 atttgatgct aaaaaattaa aaaaagaata tcttgattgg tataatcaga ccttagagtt
    18901 ttctaattta tcaaacaatg tagtaagaat agatactcct tttaaagata attctttaga
    18961 taatttaata atttacgctt tatacgatca gtccagagac atgattacac tgacagatga
    19021 cggctatact atatttgatt tagaaaataa tggtatttct ttaaataaat caaaaaaacg
    19081 taaaaagatt tttgaagagc acctttcagc ttacggtatt aaatataacg ataaaactca
    19141 cgaaattttt gttcaaacta actttaaaaa ttttaataaa tcgaaacata atttattaca
    19201 gtgccttata tttgttaatg atatgtactt actttctaat cctaagtcac agaacatatt
    19261 tacagaagat gttgcaaaca aattggatga acataacatt tattacggaa gagatttacc
    19321 tattatagga agcagtggtg ttgttcataa tttcgacttt tttattagcg ctaagaaaaa
    19381 tcaaaaagaa aaatttatca atgctatttc taaccctaat aattctatga ttattaagtc
    19441 gaaaataacg gatgctatgc aagcaaaaaa aataaaaaga cacaggcaaa atgagtttat
    19501 ttttatttta aatgactcaa aaaaagataa agtccgtata attgtgtaaa agtaaaaagg
    19561 ccatataaca gtccttttac ggtacaatgt ttttaacgac aaaaacatac ccaggaggac
    19621 ttttacatga cccaagtaca ttttacactg aaaagcgaag agattcaaag cattattgaa
    19681 tattctgtaa aggatgacgt ttctaaaaat attttaacaa cggtatttaa tcaactaatg
    19741 gaaaatcaac gaacagaata tattcaagca aaagaatatg aacgaacaga aaaccgacaa
    19801 agtcaacgaa atggctatta tgagcgcagc tttacgacac gtgtaggcac gctagaatta
    19861 aaagtaccca gaacacgtga tggccatttt tcacccacag tgtttgaacg ttatcaacga
    19921 aacgaaaaag ccctcatggc ttcaatgttg gaaatgtatg tatcaggcgt ttcaactcgt
    19981 aaagtatcaa aaattgtgga agaactttgt ggtaaatccg tctctaagtc cttcgtttct
    20041 agcttaacag aacagctaga acctatggtt aacgagtggc agaatcgttt attatcagaa
    20101 aaaaattatc cttacttaat gaccgatgta ctctatataa aagtacgaga agaaaatcga
    20161 gtactctcaa aaagctgtca tatagcgatt ggaataacca aagatggcga ccgtgaaatt
    20221 atcggcttca tgattcaaag tggcgaaagc gaagagacct ggacaacatt ttttgaatac
    20281 ctaaaagaac gcggtttaca aggtacggaa ctcgttattt ctgatgcgca caaaggatta
    20341 gtctctgcca ttagaaaatc cttcaccaac gtaagttggc aaagatgcca agttcacttc
    20401 ctaagaaata tctttaccac cattcctaaa aaaaattcaa aatctttcag agaagctgtt
    20461 aaaggaattt ttaagttcac agatattaac ttagcgcgtg aggctaaaaa tcgattgatt
    20521 catgattata tcgatcaacc aaaatattca aaagcttgcg catcattgga tgatggattc
    20581 gaagacgcct ttcaatatac cgtacaagga aattcccaca atcgactaaa gagtaccaat
    20641 ctaattgaac gactgaatca agaagtacgc agaagagaaa agattattcg catcttcccc
    20701 aatcaaacat cagccaatcg cttaattgga gccgttctta tggacctaca tgatgaatgg
    20761 atttattctt caagaaaata catcaatttt gataagtaga aatggtaaaa acattgtata
    20821 gcattttaca caggagtctg gacttgactc aaaaaaagaa ataaatgaac ataataaaaa
    20881 tcttcttcat gaaaactata ttagtacaat agattatagc gaattagatg aaaagatagg
    20941 tttattgatt taatatatat agacgtgata atatcaatgt ttatattaaa tgaaacgaat
    21001 ttgaaaattt cgaaacagct taagatagca aattgaatag ccttattgat aatgcagaat
    21061 cgtctacact tagttgaaca aattctatga gaatagatat tgttaaacta tttgggtagg
    21121 cgattttatt ttgacaagag tcagaagatt atttagttca tagtttaagt tacaaatggc
    21181 taaactttat gaaaatggta tgactagaat cgaaattata ctcgaatacg atttaacact
    21241 ctcaatcttc tcgaactgaa taaatactgg gtcattcaat catcaagaca acttaataag
    21301 tgatgataaa tgttcatacc attttgcaaa tataaccaga tactctcaac aaaatgtaga
    21361 gttcaaaatt aaaaatgtcc ctaattggca cattgctgta tgataataaa ttcaacttag
    21421 atttcaacaa tataactaat gatgcttata tttaccgcga tatcaaacca aacagcccat
    21481 ccacaaacaa attagaatat atcatgttaa taatagtaaa tgatatatct tccaaagacc
    21541 gcaaactgta gcagaacatg agaaccttgc gattttgaaa tggtctaata aatcaaatat
    21601 cattgaaact ttaatcacat aaattaacag tagtcacctg ttcgcagttc tataaattac
    21661 aaatgcaata aaggctaaag taaactaatt actttagcct ttattaataa tttagtcgaa
    21721 tttattaaaa atcgactaac tcctggttat gctacgcttt actttataat ccgcacgtag
    21781 atgccgattg attatattat aatattatta ttttattttt tcaagcgtat atataatatt
    21841 tgattttctt taagtaacag ctaaacatta catttcaaaa cgattaggtc atgcagatgt
    21901 acatacaacg cttaaaacat attcacattt gattaaagaa tttgaagata atgaaactaa
    21961 atttatagaa aaaaatctga atgatttgtt ttgtgactaa acttcaaaat cttgtgacta
    22021 aaattaggat tattcatcaa aaattagtca cattgtggct aaaaaatttt aaatggatat
    22081 aaataaataa gaagtgtaaa aatgaggata atagctcaaa tgcagatata tcaagggatt
    22141 gagtccaaaa taaaaatagc gcttaaaatg ggtaaaacca atttaagcgc ctttacggaa
    22201 ggtaagggat tcgaaccctt gagacgcttg tgacgcctac acactttcca ggcgtgctcc
    22261 ttcggccagc tcggacaacc ttcctcaaca ataaatatgt aataaaaaaa cagaagcgat
    22321 atttcacttc tgcttgtatg actcctacgg gactcgaacc cgtgttaccg ccgtgaaagg
    22381 gcggtgtctt aaccgcttga ccaaggagcc atggctccac aggtaggact cgaacctacg
    22441 accgatcggt taacagccga tagctctacc actgagctac tgtggaataa tagaaaataa
    22501 tggagcgggt gatgggaatc gaacccacaa catcagcttg gaaggctgag gttttgccat
    22561 taaactacac ccgctaatgt aaacgattat ggggcggctg aaggggatcg aaccctcgaa
    22621 tgtcggaacc acaatccgat gtgttaacca cttcaccaca accgccatga attaagaatg
    22681 gttcaggaca gagtcgaact gccgacacat ggagcttcaa tccattgctc taccaactga
    22741 gctactgaac cgtaatggta ataataatgg cggtctcgac gggaatcgaa cccgcgatct
    22801 cctgcgtgac aggcaggcgt gttaaccgct acactacgag acctattaaa ttaaaaacta
    22861 tgtattgcgg gaggcggatt tgaaccaccg accttcgggt tatgagcccg acgagctacc
    22921 gaactgctcc atcccgcgct aatattattt tgaattacct aattaatata ccataatcaa
    22981 aaacctaaag tcaagaactt tttgaattta atttaaatgt tatctcttaa ataattactt
    23041 aaatatcgta gcaacatgtt ctctgttgaa cacaaatatt agtatattca tttttgtagt
    23101 gtacgtcaac gacattttca aagttttttg tgtaaaaaaa cgcttcttat tcccttttat
    23161 catataagtg tctaatagtt gtcataaata gtgttaaagc atttaaaaag gtataggagt
    23221 tataaagttt acaacgccta taccttctga aaaagaaatt ataaaagctt gttacaccgc
    23281 atattctttc agtcagcgac taccaatata acattgtagc cctaagacat tgcttgacgc
    23341 ctcaattaca acaatttttc aaaatcagca gctacctact gacacaacat aacacaaccc
    23401 atacactaac tatcgtgtca tgtaatcttg catccgatct tgcaacgctg taaatgtttc
    23461 gaagccatcc tcttctaaga agtgccctcc atcttccacg attcgcaagt tcccctctaa
    23521 tgcattcatt aaacgctggg tctctttata tgaaacgtat ttgtcatttt tagaactcaa
    23581 tccgtaaaaa ttgtcaactt tctttttaat attatcgtaa tcaatggtta cattacttaa
    23641 atcaatatct aaatctatat tttctgcatc ttctttaaag cccgctatac tgaaaaagcc
    23701 ttcaatcggc tgatcaatca tttcaatata ttttaaagct gtgattgaac ctaaaccatg
    23761 tgttacaaaa tatgtatcct ttttgcgtac attaatttgt ttcgtcatag cttcaatcca
    23821 ctgatccact gtcttcgctt caggggattc aaaattaaat aatgttacgt catatccttc
    23881 taaagttaag ttatgctcca accactgata ccaatgattt ctactatttc catgcataga
    23941 atgtacaata attacatctg tcatctcatt ctctcctttc aacttactac ttcttttcta
    24001 tttttaaaaa aatgactgat tacctataat tgtaaaataa aaacacctta attagaaatg
    24061 ttatatcgca aagtgacatt tctaattaaa gtgtattgtc atcatttcaa tatcattcaa
    24121 aaacagctaa acctttgtct ctgcttcaat ttcacaaaaa taattcccgc tgaaagtatc
    24181 tatatttaca cattacttcc accattatat aacttaaaaa tgactatatt t
//
