LOCUS       NZ_CP077098            10261 bp    DNA     circular CON 03-MAR-2024
DEFINITION  Staphylococcus aureus strain RGB-095930 chromosome, complete genome.
ACCESSION   NZ_CP077098
VERSION     NZ_CP077098.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2904556)
  AUTHORS   Burgold-Voigt,S., Monecke,S., Simbeck,A., Holzmann,T., Kieninger,B.,
            Liebler-Tenorio,E.M., Braun,S.D., Collatz,M., Diezel,C., Muller,E.,
            Schneider-Brachert,W. and Ehricht,R.
  TITLE     Characterisation and Molecular Analysis of an Unusual Chimeric
            Methicillin Resistant Staphylococcus Aureus Strain and its
            Bacteriophages
  JOURNAL   Front Genet 12, 723958 (2021)
   PUBMED   34868203
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904556)
  AUTHORS   Burgold-Voigt,S., Braun,S.D., Kieninger,B. and Monecke,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2021) Optisch-Molekulare Diagnostik und
            Systemtechnologien, Leibniz Institute of Photonic Technology (IPHT),
            Philosophenweg 7, Jena 07743, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-FEB-2021
            Assembly Method                   :: Flye v. 2.8-b1674
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 169.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiniSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 14:14:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,951
            CDSs (total)                      :: 2,873
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 43 of 101
            Pseudo Genes (incomplete)         :: 55 of 101
            Pseudo Genes (internal stop)      :: 22 of 101
            Pseudo Genes (multiple problems)  :: 17 of 101
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:14:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1001450
            Orig. end                         :: 1011711
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP077098.1.
            Bacteria and source DNA available from Prof. Wulf
            Schneider-Brachert, Department of Infection Prevention and
            Infectious Diseases, University Hospital Regensburg, Regensburg,
            Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10261
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10261
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10261
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5261](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5261
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(186..199)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     misc_feature    complement(571..586)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.81"
                     /tool="antismash"
     tRNA            complement(598..686)
                     /anticodon=(pos:complement(1002099..1002101),aa:Ser,
                     seq:gct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KSF82_RS04820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KSF82_04815"
                     /product="tRNA-Ser"
     gene            complement(598..686)
                     /locus_tag="KSF82_RS04820"
                     /old_locus_tag="KSF82_04815"
     misc_feature    complement(624..633)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 15.28"
                     /tool="antismash"
     tRNA            complement(689..763)
                     /anticodon=(pos:complement(1002179..1002181),aa:Asn,
                     seq:gtt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KSF82_RS04825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KSF82_04820"
                     /product="tRNA-Asn"
     gene            complement(689..763)
                     /locus_tag="KSF82_RS04825"
                     /old_locus_tag="KSF82_04820"
     gene            complement(905..1474)
                     /locus_tag="KSF82_RS04830"
                     /old_locus_tag="KSF82_04825"
     CDS             complement(905..1474)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /locus_tag="KSF82_RS04830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_04825"
                     /product="competence protein ComK"
                     /protein_id="WP_000287265.1"
                     /transl_table=11
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFKV
                     QKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIWLN
                     MHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMISKNP
                     DQPIDYNKATLNVFEALTRYSLFEDK"
     misc_feature    1541..1554
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.83"
                     /tool="antismash"
     gene            1684..1902
                     /locus_tag="KSF82_RS04835"
                     /old_locus_tag="KSF82_04830"
     CDS             1684..1902
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /locus_tag="KSF82_RS04835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_04830"
                     /product="IDEAL domain-containing protein"
                     /protein_id="WP_000876826.1"
                     /transl_table=11
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELID
                     DALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(1983..2969)
                     /locus_tag="KSF82_RS04840"
                     /old_locus_tag="KSF82_04835"
     CDS             complement(1983..2969)
                     /EC_number="6.3.1.20"
                     /GO_function="GO:0017118 - lipoyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /locus_tag="KSF82_RS04840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_04835"
                     /product="lipoate--protein ligase"
                     /protein_id="WP_000668820.1"
                     /transl_table=11
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVG
                     KNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKFTE
                     PIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQNAL
                     KVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLTDEDW
                     ENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFG
                     VGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            3168..3344
                     /locus_tag="KSF82_RS04845"
                     /old_locus_tag="KSF82_04840"
     CDS             3168..3344
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /locus_tag="KSF82_RS04845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_04840"
                     /product="YkvS family protein"
                     /protein_id="WP_000214898.1"
                     /transl_table=11
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLPE
                     KTVINHKRYKIVE"
     gene            3359..3961
                     /locus_tag="KSF82_RS04850"
                     /old_locus_tag="KSF82_04845"
     CDS             3359..3961
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /locus_tag="KSF82_RS04850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_04845"
                     /product="CPBP family glutamic-type intramembrane protease"
                     /protein_id="WP_001033867.1"
                     /sec_met_domain="Abi (E-value: 1e-07, bitscore: 29.7,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAGI
                     SYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVNWK
                     WQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWIVTF
                     IFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            4277..4339
                     /locus_tag="KSF82_RS14745"
     CDS             4277..4339
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /locus_tag="KSF82_RS14745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_228764722.1"
                     /transl_table=11
                     /translation="MFIVTFIGIVVAIINLSNKK"
     gene            4878..4979
                     /locus_tag="KSF82_RS04860"
                     /old_locus_tag="KSF82_04855"
     CDS             4878..4979
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /locus_tag="KSF82_RS04860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_04855"
                     /product="hypothetical protein"
                     /protein_id="WP_001790623.1"
                     /transl_table=11
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     misc_feature    4910..4924
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 26.74"
                     /tool="antismash"
     gene            5001..5261
                     /locus_tag="KSF82_RS04865"
                     /old_locus_tag="KSF82_04860"
     CDS             5001..5261
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="KSF82_RS04865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_04860"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_130137088.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 7.8e-24,
                     bitscore: 81.5, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYYHGHKG
                     HGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            5305..7269
                     /locus_tag="KSF82_RS04870"
                     /old_locus_tag="KSF82_04865"
     CDS             5305..7269
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /locus_tag="KSF82_RS04870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_04865"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000870806.1"
                     /transl_table=11
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDWN
                     KKYSKNEIYKRIDQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKAATITPFNAKYNI
                     HYLSDDEVLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIKQMN
                     IVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTLIITQ
                     ILLTTSVLWILNYTGNLDLFILRIVLLSCLFILTISVINLWTFLMLLNLNIANMIKGKQ
                     HFKTIRFINTVCKSILLVLIANVMIENTSVIKDLNKIKETEKYWNVLDDYYTIEFAPYH
                     ETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVNNQFWSIY
                     YKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKENKLSIQFIN
                     KNDCRIFSFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFKNYDALVKNI
                     EKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIILFDVKYYFEQH
                     RKLLVIKKLYGYSTLRANYQYLLINNIVVIFIGILTNVILHSHYIMMLFATILVVQILL
                     QICSLYYHGRRFNEVIKEF"
     gene            7272..7592
                     /locus_tag="KSF82_RS04875"
                     /old_locus_tag="KSF82_04870"
     CDS             7272..7592
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /locus_tag="KSF82_RS04875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_04870"
                     /product="YxeA family protein"
                     /protein_id="WP_000668623.1"
                     /transl_table=11
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVPK
                     GTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKEVR
                     QE"
     gene            7589..8230
                     /locus_tag="KSF82_RS04880"
                     /old_locus_tag="KSF82_04875"
     CDS             7589..8230
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /locus_tag="KSF82_RS04880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_04875"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000571178.1"
                     /transl_table=11
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLLN
                     TIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYKKI
                     SKKEKEQIKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPTGAL
                     DPKTGQMIIQSLFGLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(8318..8608)
                     /locus_tag="KSF82_RS04885"
                     /old_locus_tag="KSF82_04880"
     CDS             complement(8318..8608)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /locus_tag="KSF82_RS04885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_04880"
                     /product="hypothetical protein"
                     /protein_id="WP_001795544.1"
                     /transl_table=11
                     /translation="MFYACHFKVKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTAS
                     ALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
     gene            8887..9174
                     /locus_tag="KSF82_RS04890"
                     /old_locus_tag="KSF82_04885"
     CDS             8887..9174
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /locus_tag="KSF82_RS04890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSF82_04885"
                     /product="hypothetical protein"
                     /protein_id="WP_000410720.1"
                     /transl_table=11
                     /translation="MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEVE
                     NGNTKLKLNGYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE"
     misc_feature    complement(10121..10135)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.62"
                     /tool="antismash"
ORIGIN
        1 aaatgaagat ttgtatcatg catacccata tccagataag ccaatggata tgacaattag
       61 tggtcaaaat atcaaagata tattggagta tagttattca catttagatt ttgttaacga
      121 gcaattaagc ttaacaatta ttgatgaaac gttatgtaca atgtggcaag gattcaatta
      181 tgagattgat atgaatcaag aacctgggca acgagtaatg ttagatcaaa ttgatttgac
      241 taagagttat agagttacaa tgactgacta ttgttatcgt aactacaaga attatttaaa
      301 aaatgctatt atacatgaat catacgatga aacaatgagt acattaattg cagagaagtt
      361 aagagatccg aattatcgta tttcatgtag tgataatttt atagttaaaa acaggtaaat
      421 attgctatgt aataaattaa ttataagttc aacaagtata aaaacctttc ttagagaaga
      481 ttatctagga aaggttttta gaattttaaa attgatataa aagaacagtg aaattctcat
      541 tcattatata taagtaaaca aaaaagctaa aatggtatat accattttag caagtaacgg
      601 aaacggaggg attcgaaccc tcgcgccgct ttcgcgacct acacccttag caggggcgcc
      661 tcttcagcca acttgagtac gtttccaatg gctccacagg taggactcga acctacgacc
      721 gatcggttaa cagccgatag ctctaccact gagctactgt ggattaattt gtatgtgttt
      781 gaatatcaag cacaaatatt attataacaa gattaagtta atttgcaata gataccttaa
      841 aaaaatatgc agaacattta cactatattt ataaaaaatt ccgcatattt taaaaataaa
      901 caatttattt atcttcaaat aaagaatagc gtgtcaatgc ttcaaacaca ttcaatgtgg
      961 ctttattata atctattggt tggtcgggat tttttgatat catgcgagat tgtttatcta
     1021 ccatgtaata gtaaaaaatg gaattgttat attgatgcca taaactgtgg tatgaaacat
     1081 gaagaatgat tgattcatta ttaataaatg tcactttaca tttacgattt tttaattctt
     1141 taatactttc gatataatgc atatttaacc aaatattttc attttgtctg tcagaatgtg
     1201 ttgggaaaaa ataagttgga aataatggtg ttagtaaaat aggtggttta ctagaaatgc
     1261 cagtaatgcg gtttgtctct gctttcttgc caagataagt attaccataa aatttgcaag
     1321 agcgttcaat gattttctgg actttaaaag gattttgaat acgcgttttg tcaaaccgaa
     1381 ttatttcact accgtttctt tgatcgtcat catcaaatgc tggtcgaata accatgtctc
     1441 ctttgcgtat cacataaata ttttgagaat acataagcaa aacctcgctt tattaagttt
     1501 taaaccatta atataaaaat acaattaatg atataaagat attttataat aaaattctga
     1561 gttgtcaagt tattattttg gtgaaaatgt acaataattt ttaactaata gtcagaatat
     1621 tcattgattt caaaattaga aaaatgtata cttattataa attaaagaaa ggtagtggtt
     1681 tctatgaaat acaatactaa tgttaaacat acaactttag aagcgtttgt cacaactgtc
     1741 aatgatttgg gtattgaatt aattatcaat gaagcacttc gagaggtaag aaaacgacag
     1801 ctcatagaac ttatagatga cgcactcgtc aataaagatg aagcagcatt taatcaatat
     1861 acggcagaat acaaaaattt ggaggcattt ctcggtgaat aacattgatt cgaattccat
     1921 aacgcaatca taaaaggact attcttaaca tctcatacat gtaagaatag tcctttaatc
     1981 gtttatgaca ttaatctaat taattcatgt ctatctatgt caccgaaata atgatataaa
     2041 tcatattctg atagtgcttc ttctatgtgc tcaaagtcgt gtagacagcc aactaatgca
     2101 ttttcaagat cagtgacatc tccgacacca aagaaatcac cgaatatttt tgcatgttcg
     2161 attttacctc gtttaacatc aaacttaatt tgtacaaatc ctttttcaaa tttttcctcg
     2221 cgttcaaagt tatatttagg gtttctacca taattccatt cccaagttct atatttgtcg
     2281 ttacttaact tttcaatatt ttcccaatct tcatccgtta atttatattc ttctacttca
     2341 gtttctccaa agatagtttt caagatgatt tttttaaatt cttcaatttc taaagggtca
     2401 tttaaaaatt cttgaatgtt tgctacccgt ttacgaacag atttaatacc ttttgattta
     2461 atcttagctg gattaacttt tagtgcattc tgaacttcgt ctaaatcact atttaacatc
     2521 aacgtaccat gactaaacat tctattttta actttaacca tagcattccc tgagattttc
     2581 gcttgtccaa cttgaatatc gttacgacca gttaattcag catttacgcc taaagattgt
     2641 aatgcttgaa caattggttc agtgaatttt tggaaattgt ggaaactgtt accatcatca
     2701 tctgttataa aactaaagtt taaattgcca gtatcatgat aaacagcgcc accaccagaa
     2761 attcttctta ctacatcgat gttgtgagca tcgatatatg tctgatttac ttcctctatc
     2821 gtattttgat tctttccaac aatgatagat ggtctattta tgtaaaataa aaagtaactt
     2881 tcttctgctg gtaaattttt taaaacatat tcttccattg ctaagtttaa agttggatct
     2941 gtaatattat tattactaat gaatttcatt acaatctctc ccttatatct atatatattc
     3001 tctacttatt tatgccttaa ctttgctcaa aatgccaatt atatactcac aaatcattct
     3061 aaatatcggc ttaaagttac attttattat ttttgattgt taatagttta tttgttaagt
     3121 gaattttagt acaatataat ttagttatga tttaaggagg acttaaaatg actgttgcag
     3181 aagtgggtaa cattgttgag tttatggatg gattaagagg tcgtgttgaa aaaatcaacg
     3241 ataactctgt tattgttgac ttaacaatta tggaaaattt taatgacctt gatttaccgg
     3301 aaaaaactgt tatcaatcat aaacgatata agattgttga ataagaaggt aagttataat
     3361 gaataaaatc tcgaaggctt taacttggtt tattataagt ttcattatat ttcatctcat
     3421 attatttatt atgtggggcg aacaccaaga atactggtat ttatatacag gtataatgct
     3481 aattgctggt atcagttatg tattttatca aagagatatt gaatctaagc ggttgcttac
     3541 atcaattggt gttggtatta ttacggcaat tattttaatt atgcttcaac ttttattctc
     3601 acttataact tctaatttaa gttatagttc attaattaaa gaattagcaa gaacaggtgt
     3661 caattggaag tggcaaatgt tagtaacttt actttttgtc attccatgtc acgagttata
     3721 tatgagaact gttttacaaa aagaattaac acatttttca ataccgaaat gggtagctat
     3781 attaataact gcaatttgtt ctagttcatt atttatttat ttagataatt ggtggatagt
     3841 aacatttata tttgttgcac aagtgatatt atcattaagt tatgagtata ctcgacgtat
     3901 agcaacaaca tccgttgcac aaattgtagc tattatattg ttattaattt ttaatgctta
     3961 aataagcttt tcttagacgt atgtgttcta agaaaagctt ttttgcttaa gaatagcaat
     4021 acgaagattc gaggcatgct tcattatagt ggtaatattt tttaacagca agcggttatt
     4081 attgagatta ggttttagtc agtttttatt actaaatatt ttataaaaat ggtataatat
     4141 ttttgagaaa gttgaattga tggtggctat ctgagtaaag gggggtggtg cctatggcat
     4201 tacttaacac ttttgaaagg aaaagcctat tgtgatatct attgcaaacg cattacattt
     4261 aatgttaagt ttcggtatgt ttatcgtcac tttcattggt atagtagtag caataataaa
     4321 tttaagcaat aaaaaataac catcatttca aaactttgac atagaatgat ggttaaagaa
     4381 aatctcattt agtcaccgtc tttttaacgg gctcattagg cgacatgttc ctgcatgtcg
     4441 tctttttaca tttttatagt aacatatctt ttatttaatt gtctattttt attagatgtg
     4501 taacaaaatt ttgaagtatt ttccgtatat tgcctgctaa acatgtcaga tttgaaagga
     4561 aaatcatact gtggtagaaa tcaattatga ttatatggta gtacttataa ataagtgatt
     4621 tctattgaat atgtaatgaa gttcatatat taatataact ttcattgata tagaagtcgc
     4681 aatgattaat ttaaacaata aaaaacaacc atcatttcca aactttgaca acgaatgatg
     4741 gttaaagaaa atctcattta gtcaccgtct ttttaacggg ctcaataggc gacatgttcc
     4801 tgcatatcgt ctatttatac actgcacaat ttaataataa tacaattata ctttgatgtc
     4861 catttaacat aaagtggttg aaatttgcag caaataaatt aaccttgact attttcagaa
     4921 aattctgtat aaataacgaa aaagagatat ggattaaata tgaagaggtg tttttataga
     4981 agaataaaat ttttggcact atattatatt taactttagc acttggatta tcaacagcag
     5041 cttatgcatc tacagaatac gcagaaggag gcacttggag tcatggtgtc ggcagtaagt
     5101 atgtttggtc gtattattat catggtcata aaggacatgg tgcaacagct attggaaaat
     5161 atagatcatt tagtggttat acaagagctg gtgtaaaagc aaaagcatca gctactaaac
     5221 ataattggtg ggtcaataga gcgtattata acatttatta aatactaaaa aatcatagaa
     5281 aataaacaac gcaagaggta caacatgaaa tggttcaaat taatacttga tgttacaatc
     5341 ttcattctga ttgctatatt gttatttgtt tatacatata aagaaaacga agaaatattg
     5401 cctgatacta aataccctat agcggtaact gactggaata aaaaatatag taaaaatgag
     5461 atttataaac gtatagatca attcgctaaa aatgagaacg tagcaatcta taaatcaact
     5521 tcaaattata caaacaaaaa cgtagataaa gatatatatg tatttaataa atcaaaagca
     5581 gcaactatca ctccttttaa tgctaaatat aacattcatt atttaagtga cgacgaagta
     5641 ttaaaaaaag atatcaaagg gagttatttt gtaaaagaca aaaattttga cgtgtctaaa
     5701 ttcataaatt ttttaaaaga atatggtgtt actgctgaat catacaaaat agatcatatg
     5761 atgattgccg ttggtgtcat taaacaaatg aatatagtag ttccattatc ctcactttta
     5821 atcgtttatt ttatttatta tattttcgaa aagaacatta atttcaaagc gtatgcgatt
     5881 aagtatttaa atggttttac attaagaaaa ataattttcg aaaatttttc aaagaaatgc
     5941 acgtattggg taacgttaat cataactcaa attcttttaa ctacaagtgt actttggata
     6001 ttaaattaca cgggtaattt agatttattc atattaagaa tagttctgct ttcatgtctt
     6061 ttcattttaa cgattagtgt tattaattta tggactttct taatgttact aaatttaaat
     6121 attgcaaata tgattaaagg taagcagcac tttaaaacaa ttcgttttat taatacagtt
     6181 tgtaaaagta ttctcttagt actaatagct aatgtaatga tagaaaatac tagtgttatt
     6241 aaagatttga ataaaataaa agaaactgag aaatattgga atgtattaga tgattattac
     6301 acgattgaat ttgcacctta tcacgaaaca aaacaaagtt tgattgataa tatggtgcga
     6361 tcagaacaat tagtaaaggc tagtgaagca gaaaataatg cgattttatt caaaccaaag
     6421 ggtgactccg ttgacaatga caacttttcg cctgatgagg ggaatgtaat attagtaaat
     6481 aatcagttct ggtcgattta ttacaagcag tttcaacctg atattccgat aaaaaatcaa
     6541 aaaaataatg tcgaagtaat tattccacaa aagtttcatg caatgcgtaa tgaaatcaat
     6601 caagcatatc attcatggtt tgaatttgta caaaataaaa ataataaaga gaataagtta
     6661 tctatacagt ttatcaacaa aaatgattgt cgaatttttt catttgatgc acgagatagt
     6721 cgccatttgt catttataga ggcgccaatc attgtgaatg ttcaggcatc agatttatcg
     6781 aatgattttt attatgccat gatcagtcaa ggcgggtatt tattcaaaaa ttatgacgcg
     6841 ctagtaaaaa atattgaaaa gtatcatctt gatggggaaa tcagtggaat aaccaattat
     6901 aaagatagcg tgatggaaat gtatcatgaa aacaatttga aattaacagt actcaacttt
     6961 tcacaaatca ttatcgcaat cattttaata attattattt tatttgatgt gaaatattat
     7021 tttgaacagc atcgaaaatt actcgtaatc aaaaagctat atggttattc aacattaaga
     7081 gccaattacc aatacttatt aataaataat atagttgtta tttttattgg aatattgacg
     7141 aatgtaattt tacattctca ctatataatg atgttatttg caacgattct tgtcgttcaa
     7201 atattattgc agatttgcag cctatactat catggccgac gttttaatga agttatcaag
     7261 gagttttaac tatgaaattt atcattgcaa tattattagg attaatatta tccattacta
     7321 ttgcttttac aatcatacat catcctatac ttgatcgttt taatcctttc ttaaaaacgg
     7381 agtatagtta tgccaaagtg ccaaaaggta cgcaacaata tgttaatatt acggcttata
     7441 gtgaaagagg ggaaaagctt gattataaat taacatttaa tggattttca cctagtagaa
     7501 cgtatgtcga aataaagcat aaagggcaat atgtcatatc gatcacatat gttgaaaaag
     7561 aggatacacc aaaagaggta agacaagaat gatagaatta aaagatttaa ccatacaaaa
     7621 aggtaataca catattttga agaaacttaa tttgaaattt caatgtggaa aatcatatgc
     7681 acttattggt aaaagtggat gcggtaaatc tacattatta aatactattg ctggacttga
     7741 aaaaacagga aaacaatatg tctattttaa tggtcaatta gaacaattta aatctaattt
     7801 ttacagagat aaattaggat atttatttca aaattatgga ttaatcgata atttgacagt
     7861 aaatgaaaat ttagatattg gattagcata taaaaaaata agtaagaaag aaaaagaaca
     7921 aataaagata cgttatatag aacagtttgg tctgtcaaac agtttaaaaa gaaaagttca
     7981 tacgctaagt ggaggtgaac aacaacgtgt cgctttaatt agaatgatgt taaaagatcc
     8041 gattgttatg ttagctgatg aaccaacggg tgcgttagat cctaaaacag gacagatgat
     8101 tattcaatca ttatttggtt tggtcgatga aaataaagtg ctgattttag caacacatga
     8161 tatggctatt gcaaatcaat gtgacgaaat aatagattta gaacagtata gaaaagtagc
     8221 atctatgtga tatccaaaag ataaaattaa aagccaatca atgggaaatg ttgacttcaa
     8281 cactccgttt gattggcttt tatactgtgt tactaagtta tgaaagtaac aacggcaaac
     8341 tgaaaaaaag caatataata atatttaata cgattagtaa taaatgtaat tgatttttcg
     8401 ttttaatata gcaaaaaaca atgactaaag ataacaacaa tatcatgatt gcacctggga
     8461 agaaccataa tgctgaagct gtgtaccaaa tcattggtaa cgccataata ataaaagata
     8521 taagacaaga aaggatacca ataatattat aagttctagt caaaattaca cctccaattt
     8581 tcactttaaa atgacatgcg taaaacaatc attttaaata cattatacaa taaaattaaa
     8641 ttgtgcggta gaagaatttg aaattaaatt gaattgtttt tggtagttgg tatatttata
     8701 acactctgca aaagaatgta gcaaaattag ataaattaga ttatttaaaa ttatgcgtga
     8761 tattattatt tttatcatgc aaataattat aataacaata tttaattatt gtatttacag
     8821 ttgattaata gcgttgtgaa gtataaaatt attttgtaaa acaattttgt tattaagagg
     8881 tgcgctatgg aaaaggggat tttcaattac gataatgcga atgtattaaa attagatact
     8941 aatcagttaa atgaaaacat aaaagtcatt gacgatatat ttaaaaatta tgagcagata
     9001 gagccaacta tagaagtcga aaatggaaat acaaaattaa aattaaatgg atattttatt
     9061 gcgtcaatta taagcccatt aaatttaaat aaacttaata atttatatgt tgaagaagaa
     9121 ttttatcata catataacga attaattgtt aaatatactg aggtaaagga ataattataa
     9181 tgaaaaaaat atttatgatg gtacatgagt tagatgtgaa taaaggtggt atgacctctt
     9241 cgatgttcaa tagaagtaaa gagttttatg atgcggacat acctgctgat attgttactt
     9301 tcgattacaa aggaaactat gatgaaatta ttaaagcttt gaaaaaacaa ggtaaaatgg
     9361 atcgaagaac gaaaatgtat aatgtatttg agtattttaa acaaatttca aataataaac
     9421 attttaagtc taataaattg ttatataaac atatttcaga aagactaaaa aatacgattg
     9481 aaattgaaga gagtaaaggt atttcaagat attttgatat aacgactggt acatatattg
     9541 cctacattag aaaaagtaaa tctgaaaaag tgattgattt ctttaaagat aataaacgaa
     9601 ttgaacggtt tagttttata gataataaag tgcatatgaa ggaaacattt aatgtagata
     9661 ataaagtttg ttatcaagta ttttatgatg aaaagggata cccatatatt tcaaggaata
     9721 ttaatgctaa taatggtgct gtaggtaaaa cttatgtgtt agttaataaa aaagaattta
     9781 aaaacaattt agcactgtgt gtttactatt tagaaaaact aataaaagat tctaaagata
     9841 gtattatgat ttgtgatgga ccagggagtt ttccaaaaat gtttaataca aatcataaaa
     9901 atgctcagaa atatggcgtt attcatgtta atcatcatga aaatttcgat gatacgggtg
     9961 catttaaaaa aagtgagaaa tatattattg agaatgcgaa taaaattaac ggtgtaattg
    10021 tattaacaga ggcacaaaga ttagatattc ttaatcaatt tgatgtagaa aatattttca
    10081 ctattagcaa ttttgttaag atacataatg ctccaaaaca ttttcaaact gaaaaaatcg
    10141 taggtcatat ttctagaatg gtaccaacga agcgaattga tttgcttatt gaagtggctg
    10201 agttagtcgt aaaaaaagat aatgctgtta aatttcatat atatggagaa ggatctgtca
    10261 a
//
