LOCUS       NZ_JAIFIS010000001     10393 bp    DNA     linear   CON 13-SEP-2024
DEFINITION  Paenibacillus lautus strain PLV Scaffold_1, whole genome shotgun
            sequence.
ACCESSION   NZ_JAIFIS010000001
VERSION     NZ_JAIFIS010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus lautus
  ORGANISM  Paenibacillus lautus
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 7405488)
  AUTHORS   Ptak,A., Gurgul,A., Jaglarz,A., Moranska,E., Laurain-Mattar,D.,
            Spina,R. and Simlat,M.
  TITLE     Endophytic bacteria from Leucojum aestivum L. in vitro plants, a new
            source and elicitor of Amaryllidaceae alkaloids biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7405488)
  AUTHORS   Ptak,A., Gurgul,A., Jaglarz,A., Moranska,E., Laurain-Mattar,D.,
            Spina,R. and Simlat,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2021) Center for Experimental and Innovative
            Medicine, University of Agriculture in Krakow, Mickiewicza 21,
            Krakow 31-120, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Reference-guided Assembly         :: GCA_003590055.1
            Genome Coverage                   :: 496.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019641495.1-RS_2024_09_13
            Annotation Date                   :: 09/13/2024 05:30:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,260
            CDSs (total)                      :: 7,166
            Genes (coding)                    :: 7,057
            CDSs (with protein)               :: 7,057
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 5 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 38 of 109
            Pseudo Genes (incomplete)         :: 74 of 109
            Pseudo Genes (internal stop)      :: 16 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:25:35
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 319921
            Orig. end                         :: 330314
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAIFIS010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5393](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5393
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(41..56)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 21.73"
                     /tool="antismash"
     gene            132..965
                     /gene="ehuB"
                     /locus_tag="K1X09_RS01555"
                     /old_locus_tag="K1X09_01540"
     CDS             132..965
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006212832.1"
                     /locus_tag="K1X09_RS01555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01540"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_194544665.1"
                     /transl_table=11
                     /translation="MLSLIMGVSLAACGDKGSTLEQAIEQGYITVGFANEKPYAYKEAN
                     GELTGEAVEIARVILERLGIKEMRGELTEFGSLVAGLQAERFDLITAGMFINPDRCSAV
                     LFANPEYSIGEAIAVKQGNPLGLGSYEDIANHDQAKVAVMTGAVEIGYMEKSGIPTDRI
                     VQVPDQASAISALQADRVHAITMTGPSLQAMLDSASDDSIERVADFEQPVIDGESVRGY
                     GATAFRQADTEFQEAYNAELQKMKESGELLEILQKFGFTEEELPGEMTAAALCGE"
     gene            967..1620
                     /gene="ehuC"
                     /locus_tag="K1X09_RS01560"
                     /old_locus_tag="K1X09_01545"
     CDS             967..1620
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737576.1"
                     /locus_tag="K1X09_RS01560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01545"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_194544664.1"
                     /transl_table=11
                     /translation="MPNSWIDFLPTLLKGAEITIVVAFFSAILAVVMSFAAGLCRLSKL
                     WIIRTLTAVYVEVFRGTSLLVQLFWLYFALPFIGVELPKMLAAILAIGLNFGAYGSEIV
                     RSAVLAVPKGQWEAAVALNLRPWQRLWNVILPQASLRMLPPFGNQLVELIKSTSLVYFI
                     TMADLTYQAMVLRNNYISWTTEILVLLLLMYFVIATLVSLAVRLLERKWTAGRL"
     gene            1623..2285
                     /gene="ehuD"
                     /locus_tag="K1X09_RS01565"
                     /old_locus_tag="K1X09_01550"
     CDS             1623..2285
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006212830.1"
                     /locus_tag="K1X09_RS01565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01550"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_220683839.1"
                     /transl_table=11
                     /translation="MWDWNYVWDILPQLLSALKMTVWVTICAFAVALIIGLLLAFAARS
                     RFKVLSLITKGMIEFIRNTPLLVQVFFLYYSLPLLTGISIPAFYAGVIGLGLHYSTYLS
                     EVYRSGIEGVPKGQWEAATALNYTKAQTWRSIILPQAVPPIIPVLGNYLIVMFKETPIL
                     CAITLVEMMLTAKNIVSQSFRAFEPYTMVGILFLVISYIASLLVQRLEKRMNLQRGK"
     gene            2300..3157
                     /gene="ehuA"
                     /locus_tag="K1X09_RS01570"
                     /old_locus_tag="K1X09_01555"
     CDS             2300..3157
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737575.1"
                     /locus_tag="K1X09_RS01570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01555"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_269468916.1"
                     /transl_table=11
                     /translation="MNAVVIDEQAEASKVPQQEGNPVTATGTKPIVKYTGVTKSFGDLQ
                     VLKGIDLEMQPGEKVAVIGPSGSGKTTMGRMLMTLEEPTEGTIEVDGELLWHMEEKGKL
                     VRANEKHLHRMRCNVGMVFQHFNLFPHMNVLRNVTEAPRKVLGLSKEEAEERAVSMLSK
                     VGLADKLKMYPSNLSGGQKQRVAIARALVMRPKVMVFDEVTSALDPELVGEVLEVIREI
                     AEEGEMAMMLITHEMDFAKEIADRVIFGAEGRIVEQGTPEEIFDNPQSERLQSFLQRFR
                     SNGN"
     gene            3183..3695
                     /gene="ectA"
                     /locus_tag="K1X09_RS01575"
                     /old_locus_tag="K1X09_01560"
     CDS             3183..3695
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737574.1"
                     /locus_tag="K1X09_RS01575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01560"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_071219716.1"
                     /transl_table=11
                     /translation="MAVDTDTEIVYRRPKARDGTRVWELIRDTGSLDLNSPYCYMLLGD
                     YFHDTCMIAEHEGDIIGFISAFRSPRNPETLFVWQVAVANSHRRQGVAKAMLTNLMNQK
                     ACHGVRYIEATVSPSNMASRRLFLGYAEEKSIPSTVTVGYGAEMFPDGTSHEDEPLFVI
                     GPFFNDI"
     gene            3712..5004
                     /gene="ectB"
                     /locus_tag="K1X09_RS01580"
                     /old_locus_tag="K1X09_01565"
     CDS             3712..5004
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (31-367). E-value: 3.7e-74.
                     Score: 241.7. Matches aSDomain:
                     nrpspksdomains_K1X09_RS01580_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009589525.1"
                     /locus_tag="K1X09_RS01580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01565"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_220683840.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.7e-75, bitscore:
                     252.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNQVMEKPKLTVFNKLESEVRSYCRSFQTVFDKARNAKLWDTQG
                     NEYIDFFAGAGALNYGHNNEKIREKIVDYILKDGVTHSLDMATGAKETFLTRFQEIILK
                     PRGWDHKVMFPGPTGTNAVEAALKIARKVTGRSTVLCFTNAFHGMSLGSLAVTGNSFKR
                     QGAGVDLSHSVFMPYDGYFGDDVDTMAYMEKLLDDPGSGIPLPAAVIVEAVQGEGGLNA
                     ASREWLQKLARICKDKGMLLILDDIQMGCGRTGPFFSFDDAGIEPDIICLSKSIGGFGL
                     PMAITLIKPEIDIWEPGEHNGTFRGNNLGFVAAAEALNYWKTKDFQLDIGIRENRIRQS
                     LEGVVKDYPQLGGEIRGRGMIQGVAFDKPELAGRLSEMAFEHGLIMETSGPYSEVAKLM
                     PPLTIEMNTLAEGLGIFENSMKQLAAEERLS"
     aSDomain        3805..4812
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K1X09_RS01580_Aminotran_3.1"
                     /evalue="3.70E-74"
                     /label="K1X09_RS01580_Aminotran_3.1"
                     /locus_tag="K1X09_RS01580"
                     /protein_end="367"
                     /protein_start="31"
                     /score="241.7"
                     /tool="antismash"
                     /translation="FDKARNAKLWDTQGNEYIDFFAGAGALNYGHNNEKIREKIVDYIL
                     KDGVTHSLDMATGAKETFLTRFQEIILKPRGWDHKVMFPGPTGTNAVEAALKIARKVTG
                     RSTVLCFTNAFHGMSLGSLAVTGNSFKRQGAGVDLSHSVFMPYDGYFGDDVDTMAYMEK
                     LLDDPGSGIPLPAAVIVEAVQGEGGLNAASREWLQKLARICKDKGMLLILDDIQMGCGR
                     TGPFFSFDDAGIEPDIICLSKSIGGFGLPMAITLIKPEIDIWEPGEHNGTFRGNNLGFV
                     AAAEALNYWKTKDFQLDIGIRENRIRQSLEGVVKDYPQLGGEIRGRGMIQGVAFD"
     gene            5001..5393
                     /locus_tag="K1X09_RS01585"
                     /old_locus_tag="K1X09_01570"
     CDS             5001..5393
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009589551.1"
                     /locus_tag="K1X09_RS01585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01570"
                     /product="ectoine synthase"
                     /protein_id="WP_220683841.1"
                     /sec_met_domain="ectoine_synt (E-value: 4e-50, bitscore:
                     167.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVKHLEEIVDTKDDIDTQTWNSRRLLLTKDGMGFSLNDTLIKAG
                     TETLIWYKNHVEAVYCIEGEGEIEIVGGETHPISPGMMYALDGHEKHYLRARSQMRMVC
                     VFNPPLTGAEVHDEEGTYPLLAPNLD"
     gene            5408..6316
                     /gene="thpD"
                     /locus_tag="K1X09_RS01590"
                     /old_locus_tag="K1X09_01575"
     CDS             5408..6316
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737572.1"
                     /locus_tag="K1X09_RS01590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01575"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_194544659.1"
                     /sec_met_domain="PhyH (E-value: 1.9e-33, bitscore: 115.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNNHVSALQEKELDVYPSRVHSEPRILKRQDPVVHSEWTPDAAL
                     TQEQSDFYERNGYLFLEDFFGREELSVYQAEARSLQVSARELDKDEVIREPGGNEVRSV
                     FAVHESHEVFKKLSRHPRLLAIMEYLLGSETYIHQSRINYKPGFTGKEFYWHSDFETWH
                     VEDGMPRMRALSCSIALEDNYSYNGPLMVVPGSHKEFVACIGQTPENHFKDSLRKQEYG
                     VPDHDSLTRMVQKSGIDTPVGKAGSIVIFDCNIMHGSNSNITPMPRSNIFMVYNSVENK
                     VKQPYSGQKPRPEYIATRDSL"
     gene            6313..6843
                     /locus_tag="K1X09_RS01595"
                     /old_locus_tag="K1X09_01580"
     CDS             6313..6843
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737571.1"
                     /locus_tag="K1X09_RS01595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01580"
                     /product="DUF2179 domain-containing protein"
                     /protein_id="WP_015737571.1"
                     /transl_table=11
                     /translation="MISLSLIGSIIGINVVYVSIFSLRLILMIKGRRGIASLLAMLEVF
                     VYLLGLNLVLQNLDNPFNMAAYCLGFGLGVYAGSRIEEYLALGYIVVQVIVNSVETNLA
                     MNLREKGYGVTAWEADGKDGKRLVLQVLVKRKNERRLMESLSKMSPQAFIISHEPKSFK
                     GGFWVRLTEGRKL"
     gene            complement(6974..7495)
                     /locus_tag="K1X09_RS01600"
                     /old_locus_tag="K1X09_01585"
     CDS             complement(6974..7495)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1250: DinB"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737570.1"
                     /locus_tag="K1X09_RS01600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01585"
                     /product="DinB family protein"
                     /protein_id="WP_194544658.1"
                     /transl_table=11
                     /translation="MNYPVKMYEYHVWANQAIFNRLLELPGELHNQKITSIFPTVSEAL
                     IHIYTADLGWINILSGMDMQQALTEAFQLREELESISLEDLNLRFIELSERFTAFLGQN
                     PDLERMIRLDNPYAGIRDTRLSEIMLHVVNHGTYHRGNIAAMMRQLGSSSAMTDYAFFW
                     YQSEPELVLA"
     misc_feature    7541..7554
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 19.64"
                     /tool="antismash"
     misc_feature    complement(7549..7558)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 17.22"
                     /tool="antismash"
     gene            complement(7572..8531)
                     /locus_tag="K1X09_RS01605"
                     /old_locus_tag="K1X09_01590"
     CDS             complement(7572..8531)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737569.1"
                     /locus_tag="K1X09_RS01605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01590"
                     /product="YafY family protein"
                     /protein_id="WP_220683842.1"
                     /transl_table=11
                     /translation="MKLERLISMIYMLLNNEILSASALAEKYNVSQRTIYRDIDAICAA
                     GIPVVSYQGVNGGYGIMEQYKMDKSLLGSYDVSALITILHSMSTVFEDERAMDTIQRLQ
                     TIDRSANSASQGLSMDIGSWRSYNESLRLLRRAITDKHVISFQYISAKNERIHRKVEPL
                     RLMYKFGSWYLYGFCRTRADYREFRISRISELSPSAEPFLHQHSEEHTHGPHLQEQNPH
                     DRVEQHPWEPSSDAMEVILHVSPRALARAMDQFYDVQREFFEDGSLRLTFKVQDPDSWW
                     FWSTLLSFGEDIEIIEPAGLRTLMQSKLEKMLQVYTKV"
     gene            8699..8923
                     /locus_tag="K1X09_RS01610"
                     /old_locus_tag="K1X09_01595"
     CDS             8699..8923
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006212820.1"
                     /locus_tag="K1X09_RS01610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01595"
                     /product="hypothetical protein"
                     /protein_id="WP_054404324.1"
                     /transl_table=11
                     /translation="MARTKTQKALRKAERAGTWSAVSMRKTNEDYSALSQHVRVKPTKQ
                     EQLNKIKHRKRIGDDGAFFVGFGGCDIVR"
     gene            8952..9614
                     /gene="rnhA"
                     /locus_tag="K1X09_RS01615"
                     /old_locus_tag="K1X09_01600"
     CDS             8952..9614
                     /EC_number="3.1.26.4"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rnhA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009589560.1"
                     /locus_tag="K1X09_RS01615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1X09_01600"
                     /product="ribonuclease H"
                     /protein_id="WP_194544656.1"
                     /transl_table=11
                     /translation="MAKQKYYVVWVGKVPGIYTSWGDCQQQVNQFTGAKFKAFESRSEA
                     EQAYAAGYKNYWGQQSGGKSGSSGSKNYKRNSATAAEEPGEIDYDSISVDVGTRGNPGP
                     VEYKGVDTQTGDILFSCGPISKGTNNLGEFLAIVHALAYLKKIGSTKTVYSDSMNALKW
                     LKQKKVVSTLPRDESTQEIWDLVDRAEHWLRTNTYENKVLKWQTKSWGEIKADYGRK"
     misc_feature    10163..10172
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    10352..10367
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.21"
                     /tool="antismash"
ORIGIN
        1 caaggtatct gaatatgctt aaatcctagt taatatatgg gttaacatgg gtaatttcaa
       61 catgccattt ttaagaatac caaagtcata atgaaaggtt gtgggaagtt ttgaagagag
      121 tggttgtact gatgttgtca ttgattatgg gggtgtcgct tgcggcttgc ggcgacaaag
      181 ggagtacatt ggagcaagcc attgagcagg ggtatatcac cgtgggattt gcgaatgaga
      241 agccttatgc ctataaagag gccaacgggg agctgacagg agaagcggtt gagattgcga
      301 gggttattct ggagcggctt ggcatcaagg aaatgagggg agagcttacg gaattcggat
      361 cgctggttgc cggactgcag gccgagcggt ttgatctaat taccgcaggc atgttcatta
      421 atccggatcg ctgctctgcc gtattgtttg ctaatccgga gtacagcatc ggggaagcca
      481 ttgcggtgaa gcaagggaat ccgctgggac tcggaagtta tgaggatatt gccaatcatg
      541 accaagctaa ggtcgccgtt atgaccggtg cggtcgaaat cggctacatg gaaaagtccg
      601 gcattcctac ggaccggatt gtgcaggtcc ccgatcaggc ctcggccatt agcgcccttc
      661 aggcagaccg ggtgcatgca attaccatga cggggccgtc cctgcaggcg atgctggatt
      721 cggccagcga cgacagcatc gaacgcgtgg ctgatttcga gcagccggtc atcgacggcg
      781 aaagcgttcg cggttatgga gctacggctt tccgccaagc ggataccgag ttccaggagg
      841 cttataatgc ggagcttcaa aaaatgaagg agtcgggcga gctgctggaa attttgcaga
      901 agttcgggtt cacagaagaa gagctcccgg gcgagatgac ggccgctgcc ctttgcgggg
      961 aatagcatgc cgaattcgtg gattgatttt ctccctacgc tgttgaaagg ggcggagatc
     1021 acgattgtag ttgctttctt ttcagccatt cttgcggtcg tgatgtcctt tgcagccggg
     1081 ttatgccgac tgtcgaagct gtggatcatt cgcacgttga cagccgtgta cgtggaagtc
     1141 tttcggggga cctccttgct ggtgcagctg ttctggttgt atttcgccct tccgttcatc
     1201 ggcgtcgagc ttccgaaaat gctggctgcg atcctcgcga tcgggctgaa cttcggcgca
     1261 tacggctcgg aaatcgttcg cagcgccgtg ttggccgttc cgaaaggaca atgggaagcc
     1321 gctgtagcgc ttaatctgag gccttggcaa agactgtgga atgtcatatt gcctcaagcg
     1381 tccctgcgaa tgcttccccc tttcgggaat cagcttgtgg agctcatcaa atccacctca
     1441 ctggtttatt tcatcacgat ggcggattta acgtatcagg cgatggtgct ccgcaataat
     1501 tacatctcct ggacgaccga gattctggtg ctgctgctcc tcatgtattt tgtcattgct
     1561 acgctcgttt cgctggcagt tcgactgctg gagcgtaaat ggacagcggg gaggttatag
     1621 tcatgtggga ttggaactat gtatgggata tcctgcccca gctcttgagt gcattaaaaa
     1681 tgacggtatg ggtgacgatc tgcgctttcg cggtggcatt gatcatcgga ttgctgcttg
     1741 cttttgcagc gcggagtcgg tttaaggtgc tgtcactaat aaccaaagga atgatcgaat
     1801 tcattcgcaa tacaccgctg ctggtgcagg tcttcttcct gtattacagc ctgccgcttc
     1861 ttaccgggat ctccattccg gccttctatg ccggggtgat cggtctcggg ctgcattaca
     1921 gcacatatct gtccgaggtg taccgatccg gaatcgaggg agtgccgaag ggacaatggg
     1981 aggctgccac agcactgaat tacacgaaag cccaaacctg gcgaagtatt atcctgccgc
     2041 aagcggtgcc gccgatcatt ccggtactcg gtaattattt gattgttatg tttaaagaga
     2101 cgccgattct atgcgccata acgcttgtgg agatgatgct gacggccaaa aatatcgtgt
     2161 cgcagtcgtt ccgtgcattt gaaccgtata cgatggtcgg gattctgttc ctggtcatca
     2221 gttatatcgc ctcattgctt gtgcaaaggc tggaaaaacg catgaatctg cagcgtggaa
     2281 aataaaagga gggaatcaat tgaatgccgt tgtgatcgat gagcaggcgg aagcgtcgaa
     2341 ggtgccgcag caggagggaa atcccgttac cgctacgggt acaaagccga ttgtgaaata
     2401 tacaggcgtg accaagtcgt ttggtgatct acaggtgctt aaaggcatcg atctggagat
     2461 gcaacctgga gaaaaggtgg ctgtgatcgg tccgagcgga tcgggcaaaa caacgatggg
     2521 ccggatgctg atgacgctgg aagaacccac cgagggaacc atcgaggttg atggtgagct
     2581 gctctggcat atggaagaga agggcaagct ggttcgggcc aatgaaaagc atttgcaccg
     2641 gatgcgctgc aatgtcggca tggtctttca gcatttcaat ctgttcccgc acatgaacgt
     2701 gcttcggaat gtgacggagg ccccgcgcaa agtgctgggt ttatccaagg aggaggcgga
     2761 ggagcgggcc gtctccatgc tcagtaaggt cggtttggcc gacaaattga agatgtaccc
     2821 gtccaatctg tccgggggcc agaagcagcg ggttgccatc gccagagcgc tggtcatgcg
     2881 gccaaaggtt atggtgtttg acgaggtgac ctcggcgctt gatcccgagc tggtcggcga
     2941 agtgctggag gtcatccggg aaatcgcgga ggaaggcgaa atggccatga tgctgattac
     3001 gcatgagatg gattttgcga aggaaatcgc cgaccgcgtc atcttcggag ccgagggaag
     3061 gatcgttgag cagggcacgc cggaggagat attcgataat ccccagagcg agcggctgca
     3121 gagcttcctg cagcgattcc gttccaacgg aaattagttc tggaaagagg tgattgtata
     3181 gaatggctgt ggataccgat accgaaattg tttaccgaag accgaaagcc cgggatggta
     3241 cccgggtgtg ggagcttata cgcgacaccg gatcgcttga tctgaactct ccctattgct
     3301 acatgctgct tggagattac ttccatgata cctgcatgat tgccgagcat gaaggagata
     3361 tcatcggttt tatttccgcc ttccgttctc ctcgcaatcc ggagacgctg ttcgtatggc
     3421 aagtggccgt tgctaattcg catcggaggc aaggcgttgc aaaggcaatg ctgacaaatc
     3481 tgatgaatca gaaggcatgc catggcgtcc gttacattga agctacggta tcgccgagta
     3541 acatggcgtc ccgtcgtttg tttctcggtt atgccgagga gaaatccatt cccagcaccg
     3601 taaccgtagg ttatggagca gagatgttcc ccgatggaac gtcccatgaa gatgagccgt
     3661 tattcgtgat tggccccttt ttcaatgaca tttaaacaaa tggagggaac tatggacaac
     3721 caagtcatgg agaaaccgaa gcttactgtg tttaacaagc tggaatcgga agtaagaagt
     3781 tattgccgga gctttcagac ggtttttgac aaggcccgaa atgcgaagct gtgggatacg
     3841 caggggaatg aatacattga tttttttgcg ggagccgggg ctttgaatta cggacataac
     3901 aatgagaaga ttcgcgagaa gatagtcgac tacatcctga aggatggcgt cacgcatagc
     3961 ctggatatgg ctacgggtgc gaaggaaacg tttctgaccc gatttcagga gatcatcctg
     4021 aagccgcgcg ggtgggatca caaagtgatg tttcccggtc cgaccggaac gaatgccgta
     4081 gaggccgcgc tcaaaatcgc ccgtaaagtg acagggcgca gcaccgttct gtgcttcacg
     4141 aatgcctttc atggcatgtc gctcggatca ctggcggtaa ccggcaactc cttcaagcgc
     4201 cagggtgccg gcgtggatct aagtcactcc gtgtttatgc cgtatgacgg ttactttggt
     4261 gacgacgtgg atacgatggc atatatggag aaacttctgg atgatccggg aagcggaatt
     4321 ccgctgccgg ccgcagtgat cgtcgaggcg gtgcaggggg aaggcggatt gaacgccgcc
     4381 agccgggaat ggctgcagaa gcttgcgcgc atctgcaagg ataagggaat gctgctgatt
     4441 ttggatgata tccagatggg ctgcggccga accggtccgt tctttagttt tgatgatgcc
     4501 ggtattgaac cggatatcat atgcctctcg aaatcgattg gcggctttgg cttgccgatg
     4561 gcgattacgc tgatcaaacc ggagattgat atttgggagc cgggggagca taacggtacc
     4621 ttccggggca ataatctcgg attcgttgcc gcagccgaag cgctgaatta ttggaaaacg
     4681 aaagattttc agcttgatat cggcatcagg gaaaatcgga ttcgccaatc attggaaggc
     4741 gttgtgaagg attatcctca gctcggcggc gaaatccgcg gcagaggcat gattcagggg
     4801 gttgctttcg ataagccgga gctcgcaggc aggctgtccg agatggcctt cgagcatgga
     4861 ctgattatgg aaacgtccgg tccgtatagc gaagtggcaa agctaatgcc gccgcttacg
     4921 attgaaatga acacattggc agagggattg gggatatttg agaacagtat gaaacaatta
     4981 gcagcagagg agaggttgtc atgattgtta aacatttgga agagatcgtg gataccaaag
     5041 acgatatcga tacccagaca tggaactcga gaaggcttct gcttacaaaa gacggcatgg
     5101 gcttctcgct caatgatacc ctcatcaagg cgggaacgga gacgctgatc tggtacaaaa
     5161 accacgtcga agccgtgtac tgcatcgaag gcgaggggga aatcgagatt gtcgggggtg
     5221 aaacgcaccc tatttcccca ggcatgatgt atgcgctgga cggtcacgag aagcattatt
     5281 tgagagctcg ttcgcagatg cggatggttt gcgtattcaa tccgccgctt accggcgcgg
     5341 aagttcatga cgaggaagga acctacccgc tgcttgcccc aaatttggac taaggagatg
     5401 atatgcgatg agtaataatc atgtgtcggc ccttcaggaa aaagaactgg acgtttatcc
     5461 ttcaagagtg cattcggaac cccggatcct aaagagacag gatcctgtcg ttcattcgga
     5521 atggacaccg gatgctgcgt taacgcagga gcaatccgac ttctatgagc gcaacggata
     5581 tttattcctg gaagacttct ttggtcgaga ggagctgtcg gtatatcagg cggaagcccg
     5641 cagtttgcag gtatcggcaa gagagctgga taaggatgaa gtgatccggg agccgggtgg
     5701 gaacgaagtt cgatcggtct ttgcggtgca cgagagccat gaagtgttca agaagctgtc
     5761 ccggcatccg agactcttgg cgatcatgga gtatctcttg ggaagcgaga cgtatatcca
     5821 tcaatcgcgg attaattaca agccgggctt cacgggcaaa gagttctatt ggcactctga
     5881 tttcgaaaca tggcatgttg aggatggcat gcctcgtatg agagctttga gctgctccat
     5941 tgctctggag gataactact cttataacgg accgcttatg gttgtgcctg gatcgcataa
     6001 ggaattcgtg gcctgcatcg gccaaacgcc ggagaatcac ttcaaggatt ccctgcgcaa
     6061 acaggaatac ggcgttccgg atcatgacag cctgacacgc atggtgcaga aaagcggtat
     6121 cgacacacct gtcggtaaag ccggttcaat cgtgattttt gactgcaata ttatgcatgg
     6181 ttcgaacagc aatattacgc cgatgccgcg cagcaatatc tttatggttt acaacagcgt
     6241 ggagaataag gtcaagcagc cgtattcggg ccagaagcca agaccagagt acatcgccac
     6301 ccgcgattct ttatgatctc gctgtcactt atcggctcca tcatcggcat caacgtggtt
     6361 tatgtctcga tcttttcgct gcgtctgatt ctgatgatca aaggcaggag agggatcgcc
     6421 tctctcctgg ccatgctgga ggtattcgtt tacttgctcg gccttaatct cgtcctgcaa
     6481 aatctggaca atccctttaa tatggcggcc tattgcctgg gcttcggcct tggcgtatat
     6541 gccggcagtc ggatcgaaga gtatctggca ctcgggtaca tcgtggtaca ggttatcgtg
     6601 aattccgttg agacgaacct ggcgatgaac ctgcgggaga aggggtatgg cgtgactgcc
     6661 tgggaagcgg acggtaagga tggcaaacgg ctcgtgcttc aagttctcgt gaaacggaag
     6721 aatgagcggc ggttgatgga gtcactatcc aaaatgtcgc cgcaggcgtt cattatctct
     6781 catgaaccga agagctttaa aggcggcttc tgggtacgat tgacggaggg acgcaaattg
     6841 tagcacgctt ttaactagaa acaggaaagc ctgcttcgga cccgttcgga gcaggttttt
     6901 tggctgcggc ttttgccggc ctgaaccggt atggaattcg gatcagggga ttgccgatat
     6961 tgctgcgagt tatttaggca agcaccaact ccggttcact ctggtaccaa aaaaaagcgt
     7021 agtcggtcat cgcggaggag cttccgagct ggcgcatcat ggcagcaata tttccacgat
     7081 gataggtgcc gtggttcacg acatgaagca tgatctcgga gaggcgggtg tcccggatcc
     7141 cggcataagg attatccaat cggatcatcc gttccaggtc aggattttgg cccagaaagg
     7201 ccgtgaatcg ttcggatagt tcgataaacc ggaggtttag gtcctccagt gaaatggact
     7261 caagctcttc ccgaagctgg aaggcctctg ttaaagcctg ctgcatatcc atcccggaca
     7321 aaatgttgat ccatccaaga tccgccgtgt aaatgtgaat caacgcctcg gatacggttg
     7381 ggaaaatgct cgttattttc tgattgtgca attcccccgg cagctccagc agacggttga
     7441 atatcgcttg gttggcccat acatggtact catacatttt tacaggataa ttcatcgtga
     7501 tcgtcccctt tggcttcttc gtttttgctt tcttgggaac attgtaaata aaaaattatg
     7561 acagcggact gtcatacttt tgtgtatacc tgcagcattt tttctaattt ggattgcatg
     7621 agcgtcctta agccagccgg ctcaatgatc tcgatatcct caccaaagct tagcagggtc
     7681 gaccagaacc accaggaatc cggatcttgg accttgaacg tgagacggag cgagccgtct
     7741 tcaaaaaatt cccgctgtac atcatagaat tgatccatcg ctctggccaa ggcccggggg
     7801 gatacgtgaa ggatgacttc catcgcatcg gatgaaggct cccagggatg ctgctccact
     7861 cggtcgtgcg gattttgctc ttgaagatga ggaccgtgcg tgtgctcctc cgaatgctgg
     7921 tgcaaaaagg gctcggcgga gggagacagc tctgatattc tcgaaattcg aaattcgcgg
     7981 taatcggccc ttgttctgca gaaaccgtac aaataccagg agccgaattt atacatgagt
     8041 cgcaggggct cgactttacg gtgaatgcgt tcattcttgg cgcttatgta ttgaaaggag
     8101 atcacatgct tgtcggtaat ggcgcggcga agaagtcgga ggctttcgtt gtaagagcgc
     8161 cagctcccga tatccatgga caatccctgc gatgccgaat tggcggaacg atcaatggtc
     8221 tgcaaacgct gaatggtatc catggcccgt tcatcctcaa acaccgtgga catgctgtgc
     8281 aggatggtga tcaaggcgct gacatcgtag gagccaagca agcttttgtc catcttgtat
     8341 tgctccataa tgccataacc gccgttcact ccctgatacg atacaacggg aatgccggct
     8401 gcgcagattg catcaatgtc gcggtatatg gttcgctggg acacgttata tttctctgcc
     8461 agggcagacg cggacaggat ctcgttgttc agcagcatgt aaatcatgga tatgagccgc
     8521 tctagtttca tatcgcttca acccctctta tcttaattgg cctaatctcc aatgattccg
     8581 tattatatca taggttttct gaagaaggag aggaaatccc agactgcctt ttgttccatt
     8641 cccatccatt ttgccgtaca atataaggga attctataat acaaggagtg tgagccttat
     8701 ggcccgtacc aaaacgcaaa aagcgctccg caaagcggaa cgtgccggaa catggtcagc
     8761 cgtctcaatg agaaagacca acgaagatta cagtgcgctg tcacagcatg tgagagtaaa
     8821 gccgacgaaa caggaacaat tgaataagat caaacacaga aagcggatcg gagacgatgg
     8881 cgcttttttt gttggtttcg gaggatgtga tatagtacgc tagagaggtt agttcaggat
     8941 ggaggtttag gatggcgaag caaaaatatt atgtagtctg ggtaggtaaa gtgcccggaa
     9001 tttatacaag ctggggcgac tgccagcagc aggtcaatca gtttacaggt gcgaagttca
     9061 aggcgtttga atcccggagc gaagcggaac aggcctatgc ggccggttat aaaaattact
     9121 gggggcagca gtccggaggt aaatcgggca gctcaggctc caaaaactac aagcgaaaca
     9181 gcgcaactgc tgccgaagaa ccgggtgaga tcgattatga cagcatttcc gtagatgtgg
     9241 ggacgcgagg aaacccggga cccgtcgagt acaaaggcgt ggatacgcag acgggagata
     9301 ttctgttctc gtgcggaccg atttcgaagg gaacgaacaa tctcggcgag tttctggcca
     9361 tcgtccacgc actggcttat ttgaagaaaa tcggcagcac caagacggtg tacagcgatt
     9421 ccatgaatgc gctcaaatgg ctgaagcaga agaaggtggt ctccacgctt ccgcgcgatg
     9481 agtcaaccca agagatctgg gatctggtgg accgggccga gcactggctt cgaaccaata
     9541 catatgagaa caaggtgctg aaatggcaaa cgaagagctg gggagagatt aaggccgatt
     9601 acgggcgcaa gtaacgaggg agagcagaag ggagccgttc gaagctatat aaagggtggt
     9661 tttagatatc gacgtcttta tgagcggacg ggcaccttac tattaaaaag cgaagacagg
     9721 gaggaggctt ccctgttctc gctttttttt atatgccatg attccgggtt catgcggaaa
     9781 aatccaaatc aacgcatgtg gataattaac acaaaagact tacctcacat cggaagaact
     9841 gtgcataaca tcttgaataa gttcgttata cacagagtta tgaacattat ccccgttcgt
     9901 tccacacaaa ctgaacttcc gattttttat ttcaaggaag ttatccagcg tttgtgaaca
     9961 aatgtggaga agtgcaagga taactcccta ttaacttgga ggggtggcgg gttatcccca
    10021 acgccgaagg tcaaccgtta aacgcttcct tgttctatcc acgacgatac gacgaccata
    10081 acgcagggag cagcaacggg taagccaagc ttattccttc tcgcggacct gcttatagta
    10141 ttgattcggg gacatgccga cgtatttttt gaagatcttg ctgaaataga atggatcctc
    10201 cagcccggtc agatacccga tctcaccgaa gctaaggtgc gtggcctcga ttaattcctt
    10261 tgcatgattg attcgtacct gattcacata tcgcatgatc gtatgaccgg taagcttggc
    10321 gaatacccga ttgatgtaat cataattgca ttcgaagcgc tgttcgatat ccgagctgat
    10381 gattttctcg gcg
//
