LOCUS       NZ_JAFKPO010000004     20354 bp    DNA     linear   CON 15-DEC-2024
DEFINITION  Bacteroides fragilis strain FSHXHZ1E1 Scaffold4, whole genome
            shotgun sequence.
ACCESSION   NZ_JAFKPO010000004
VERSION     NZ_JAFKPO010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Pseudomonadati; Bacteroidota; Bacteroidia; Bacteroidales;
            Bacteroidaceae; Bacteroides.
REFERENCE   1  (bases 1 to 554837)
  AUTHORS   Wang,C., Li,S., Hong,K., Yu,L., Tian,F., Zhao,J., Zhang,H., Chen,W.
            and Zhai,Q.
  TITLE     The roles of different Bacteroides fragilis strains in protecting
            against DSS-induced ulcerative colitis and related functional genes
  JOURNAL   Food Funct (2021) In press
   PUBMED   34308455
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 554837)
  AUTHORS   Wang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2021) School of Food Science and Technology,
            Jiangnan University, 1800th Lihu Avenue, Wuxi, Jiangsu 214122, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 2.3 OR MAY-2011
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019633825.1-RS_2024_12_15
            Annotation Date                   :: 12/15/2024 11:10:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,946
            CDSs (total)                      :: 3,876
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 26 of 69
            Pseudo Genes (incomplete)         :: 50 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 19 of 69
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:57:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 16334
            Orig. end                         :: 36688
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAFKPO010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20354
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RRE-containing"
                     /region_number="1"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /tool="antismash"
     cand_cluster    1..20354
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="single"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20354
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10354](-)"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10354)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     misc_feature    84..98
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.9"
                     /tool="antismash"
     gene            237..1577
                     /locus_tag="K5L50_RS10655"
     CDS             237..1577
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785482.1"
                     /locus_tag="K5L50_RS10655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5690 family protein"
                     /protein_id="WP_220654180.1"
                     /transl_table=11
                     /translation="MDNVQQVKTNSQKKLSDFLFILWAGGAALLSYSLVYALRKPYTAA
                     GFDGLETFGMDYKVVVTIAQILGYVLSKFIGIKLISELKRENRMKFILISIVLAEASLI
                     LFGLLPAPYNIGAMFLNGLSLGCMWGIIFSFIEGRRMTDILASLLGVSMVISSGTAKSA
                     GLFVMDTLNISEFWMPALIGGIALPILALLGYSLNRLPQPTAEDIAMKSKRETLNGKQR
                     WDIFKNFMPFLTLLFIANVVLTILRDIKEDFLVKIIDVSQYSSWMFAQVDSVVTIIILV
                     IFGLMVFVRSNLKALSILLGLIIISMVVMVVVSFGYEQLQLNAIVWLFIQSLCLYLAFL
                     TFQTIFFDRFIACFKIRGNVGFFIAMNDFLGYTGTVIVLAVKEFFSPDINWTTFYNLMA
                     GYVGVICFVAFVCSFIYLHQRYRRENYGKTGVFRKKEEEKEVPDFVY"
     gene            1621..3132
                     /locus_tag="K5L50_RS10660"
     CDS             1621..3132
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109230.1"
                     /locus_tag="K5L50_RS10660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="WP_032539640.1"
                     /transl_table=11
                     /translation="MKIFPSSSIKKLDAYTIEHEPIASIDLMERAAQALTKAITERWDI
                     TTPVTVFAGPGNNGGDALAVARMLAEKEYKVEAYLFNPKGELSADCQTNKELVEMMDNV
                     KFSEVSTQFVPPTLTMDHLVVDGLFGSGLNKPLSGGFAAVVKYINASPATVVAIDIPSG
                     LMGEENTFNVKANIIRAHLTLSLQLPKLAFLFAENAEFVGEWKLLDINLSHEAIEETES
                     NYALLEAEEIHALIKPRSPFSHKGNFGHALLIAGSYGMAGASVLAARACMRSGVGLLTV
                     HAPMRNNDILQISVPEAIVELDASDSCFAFPTDTDDYQAVGIGPGIGRSEETEAALLEQ
                     LSGCQTPLVLDADALNILANHRHALTTLPKGSILTPHPKELERMVGKCQNSYERLMKAC
                     ELARTAKVHIILKGAYSAIITPSGKCYFNSTGNPGMATAGSGDVLTGVVLALLTQGYPA
                     EEAAKIGTYVHGLAGDFARKKQGVIGMTAGDIVNYLPMAWRLVSE"
     gene            3206..4261
                     /locus_tag="K5L50_RS10665"
     CDS             3206..4261
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775831.1"
                     /locus_tag="K5L50_RS10665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4831 family protein"
                     /protein_id="WP_005775831.1"
                     /transl_table=11
                     /translation="MKKLIILTGLLLSASAYAQTEVTAGVTRGKDYGVTYALPKTAIDI
                     EVKANKVTYTPGEFSKYADRYLRLTDVSGEPQEYWELVSVKAKSVGIPDSDHTYFVKLK
                     DKTVAPLIELTEDGIVKSINVPLSPKKSAPIQPATTLRKKVNPRDFLTEEILMAGSTAK
                     MAELVAKEIYNIRESKNALVRGQADNMPKDGEQLKIMLANLEEQEAAMTEMFSGTLNKD
                     ERIFTIRLNPEKEMDNEVAFRFSRKLGIVANNDLAGEPVYITLKNLKTVNVPEDDGKKK
                     VDGIAYNVPGKAQVTLIEGKKEWFNGELPVTQFGTIEYLAPALFNKKSTVQVTFNPATG
                     GLIKVDREEGE"
     gene            4487..5014
                     /locus_tag="K5L50_RS10670"
     CDS             4487..5014
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K5L50_RS10670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_181996436.1"
                     /transl_table=11
                     /translation="MKKFLPEADLSKLRTILDIKNSYNCGTLSLEDARKLLKEKVVVLK
                     LYEITFAEQELKGGEENECRKEDIQRMLELFNGIMDTSRPDLPPEYSVMYYYRGNDELR
                     KILPSIEGLVPPPYPTNPEQNQYPVYLVEGLIGGISSFERQDGGDQPQVQTAQVPFGAG
                     YTTQSHHQHDNR"
     gene            complement(5174..6526)
                     /locus_tag="K5L50_RS10675"
     CDS             complement(5174..6526)
                     /EC_number="1.6.5.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659319.1"
                     /locus_tag="K5L50_RS10675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_220654181.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.1e-46, bitscore:
                     154.1, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSFNIARNHNQKRIIIAGGGFGGLKLAGELRKSDYQVILLDRNNY
                     HQFPPLIYQIASSGLEPSSISFPFRRIFRKRKNFYFRMAELRGIYPEKKIIQTSIGKID
                     YDYLVLALGTTSNFFGNSRIEEEAMPMKTVSEAMGLRNALLSNLERSITCSTEQEKQEL
                     QNIVIVGGGATGVEIAGALSEMRKYVIPADYPDMDSSRINIFLIEAAGRLLSGMSAESS
                     ARAEQFLRNMGVNICLHKRVVDYRDHKVILEDGMAIPTRSFIWVSGVKAVPVGNMPAEC
                     IGRGGRIKVDAFNRVEGVENVFAIGDQCIMTADRKYPEGHPQLAQVAIQQGRLLAANLK
                     RMARKKPMRPFGYRDLGSMATVGRNRAVAEFNRVRTQGLMAWVLWLVVHLRSILGVRNK
                     VNVLLNWMWNYFTYDQSLRIIVYARKAKEIREREEREAKAHWGDEMKVPRR"
     gene            complement(6697..7713)
                     /locus_tag="K5L50_RS10680"
     CDS             complement(6697..7713)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302159.1"
                     /locus_tag="K5L50_RS10680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="inorganic phosphate transporter"
                     /protein_id="WP_220654182.1"
                     /transl_table=11
                     /translation="MELLVIIIVLALIFDYINGFHDAANSIATIVSTKVLTPFQAVIWA
                     AFFNFLAFFIAKYVIGGFGIANTVSKTVVEPYITLPVILAGVIAAIVWSLFTWWKGIPS
                     SSSHTLIGGFAGAAIMAHGFEAIQLNIILKIAVFIFLAPLIGMVIAFGVTLLVFYICKR
                     AHPHTAEVWFKKLQLVSSAMFSIGHGLNDSQKVMGIIAAAMIAGHSESLGMGINSIDDL
                     PDWVAFSCFTAISLGTMSGGWKIVKTMGTKITKVTPLEGVIAETAGAFTLYLTEYLKIP
                     VSTTHTITGAIIGVGATKRLSAVRWGVTKNLMTAWVLTIPVSGLLAAAVYLIAASFL"
     gene            complement(7727..8374)
                     /locus_tag="K5L50_RS10685"
     CDS             complement(7727..8374)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302161.1"
                     /locus_tag="K5L50_RS10685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF47 domain-containing protein"
                     /protein_id="WP_122142850.1"
                     /transl_table=11
                     /translation="MKNSFFSRFTPKEPKFFPLLKQLSEILTASSELLVEGMKHDSPEK
                     RAIFYKRIKDMEREGDKLTHRILDELGTTFITPFDREDIHDLASGIDDVIDGINSCAKR
                     IAIYNPHPISESGKELSRMIQQEAEYIVKAMDELETFRQNPNTLRGYCSKMHDIENHAD
                     DVYELFITKLFEEEKDCIELIKIKEIVYELEKTTDAAEHVGKILRSLIVKYA"
     gene            complement(8393..9043)
                     /locus_tag="K5L50_RS10690"
     CDS             complement(8393..9043)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1188: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930866.1"
                     /locus_tag="K5L50_RS10690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DedA family protein"
                     /protein_id="WP_122142851.1"
                     /transl_table=11
                     /translation="MDFLLDFILHVDQYMIDIVQEYHTWAYVILFLIIFCETGLVVTPF
                     LPGDSLLFVAGAIAALPDMLVEVNVLVLILFAAAVLGDSCNYMIGYFFGRKLFRNPDSR
                     IFKQSHLEKTHEFYKKYGGKTIILARFVPIVRTFAPFVAGMGKMHYYYFMLYNLTGGVL
                     WVAIFCYAGYFFGDLPFVQKNLKLLIVAIIVVSVLPAVIEVIRARLKTRKQTK"
     gene            complement(10001..10354)
                     /locus_tag="K5L50_RS10695"
     CDS             complement(10001..10354)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: PqqD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818687.1"
                     /locus_tag="K5L50_RS10695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PqqD family protein"
                     /protein_id="WP_005775830.1"
                     /sec_met_domain="PqqD_RRE (E-value: 1.8e-10, bitscore:
                     35.1, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 2e-16, bitscore: 54.7,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADAKEKINLLDVIPFRSENITAEKGSDGTVTIAFPRFKYEWMRR
                     FLLPKGMSPDIHVRLEEHGTAVWELIDGKRTVRQIIEELAEHFDHEENYESCITAYITQ
                     LQKDGFVKLAVTN"
     aSDomain        complement(10019..10201)
                     /aSDomain="PqqD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing protein in a pyrroloquinoline
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_K5L50_RS10695_PqqD_RRE.1"
                     /evalue="1.30E-11"
                     /identifier="RREFam006.1"
                     /label="K5L50_RS10695"
                     /locus_tag="K5L50_RS10695"
                     /protein_end="112"
                     /protein_start="51"
                     /score="35.1"
                     /tool="antismash"
                     /translation="MSPDIHVRLEEHGTAVWELIDGKRTVRQIIEELAEHFDHEENYES
                     CITAYITQLQKDGFVK"
     gene            complement(10361..11749)
                     /locus_tag="K5L50_RS10700"
     CDS             complement(10361..11749)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301891.1"
                     /locus_tag="K5L50_RS10700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_005775829.1"
                     /transl_table=11
                     /translation="MTEQLRNKLSDSKTLRWSVLALVAFTMLCGYFLTDVMSPLKPMLE
                     KELLWDSLDYGFFTSAYGWFNVFLLMLIFGGIILDKMGVRFTGMGACILMVLGCGLKYY
                     AISTTFPEGALIMGFKTQVFLAALGYAIFGVGVEIAGITVSKIIVKWFKGKEMALAMGL
                     EMATARIGTTLAMVLTVPIADYFGYTDESGSFHTNIPMPILLCLIMLCIGTIAFFIYTF
                     YDKKLDASLDAQGEEPEEPFRMKDVMLIVTNKGFWLIALLCVLFYSAVFPFIKYATDLM
                     VQKYNVDPKLAGNIPGLLPIGTIFLTPLFGTLYDRIGKGATLMIIGAVMLIGVHTLFAL
                     PILNVWWFATVIMIILGIAFSLVPSAMWPSVPKIIPEKQLGTAYALIFWVQNWGLMGVP
                     LLIGWVLNTYCKGPVVDGAQTYDYTLPMSIFACFGVLALIVALMLKAEDKKKGYGLQEA
                     NIKK"
     gene            complement(11920..12300)
                     /gene="secG"
                     /locus_tag="K5L50_RS10705"
     CDS             complement(11920..12300)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="secG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775827.1"
                     /locus_tag="K5L50_RS10705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="preprotein translocase subunit SecG"
                     /protein_id="WP_005775827.1"
                     /transl_table=11
                     /translation="MYLLLVILMVIAAILMCFIVLIQNSKGGGLASGFSSSNQIMGVRK
                     TTDFLEKATWGLAAFMVVMSIATAYVVPTSSSKTQDVIMEQAQQEEQTNPYNLPVGTTA
                     PKTDAAAPAEAPATETPATPAN"
     gene            complement(12305..13069)
                     /locus_tag="K5L50_RS10710"
     CDS             complement(12305..13069)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785470.1"
                     /locus_tag="K5L50_RS10710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_005809945.1"
                     /transl_table=11
                     /translation="MISANLQQWIQHPETLNKDTLYELRTLVTRYPYFQSLRLLYLKNL
                     YLLHDISFGAELRKAILYVADRRKLFYLIEGERYILKPRKKNMPSEAEVLEEEPGLDRT
                     LSLIDAFLATVPEEVSGQTSLDYSTDYTTYLLQEDEEDSAPEQSEIPKLRGHELIDGFI
                     ERSEGETSIRLQPAANNETLPVEEEGEAHHEEDEDDSCFTETLAKIYVKQHRYSKALEI
                     IKKLSLKYPKKNAYFADQIRFLEKLIINAKSK"
     gene            complement(13075..13596)
                     /gene="lptE"
                     /locus_tag="K5L50_RS10715"
     CDS             complement(13075..13596)
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /gene="lptE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760046.1"
                     /locus_tag="K5L50_RS10715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LptE family protein"
                     /protein_id="WP_005775825.1"
                     /transl_table=11
                     /translation="MDWNKKIMRISLLVVTLVVGISCTVSYKFNGGNINYDKVKTISIA
                     DFPIKSDYVYAPLGTKFNEDLKDIFLRQTRLKLVNNNADLQIDGEITGYNQYNQAVSAD
                     GYSSETKLTITVNVRFVNNTNHEQDFEQQFSAFRVYDSRELLTAVQDGLIAEMTKEITD
                     QIFNATVANW"
     gene            complement(13583..14809)
                     /locus_tag="K5L50_RS10720"
     CDS             complement(13583..14809)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1112: sigma-54
                     dependent trancsriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /locus_tag="K5L50_RS10720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="WP_005775824.1"
                     /transl_table=11
                     /translation="MTKAEIQQVKLRFGIIGNTEALTRAIDVAIQVAPTDLSVLITGES
                     GVGKESFPQIIHQYSRRKHGQYIAVNCGAIPEGTIDSELFGHEKGAFTGAIGERKGYFG
                     EADGGTIFLDEVGELPLPTQARLLRVLESGEFIKVGSSKVQKTDVRIVAATNVNLTQAI
                     AEGRFREDLYYRLNTVPIQIPPLRERGEDVLLLFRKFASDFAEKYRMPAIQLTEDAKQV
                     LLSYSWPGNVRQLKNITEQISIIETNREINASILRTYLPAQNTQRLPALFGVKAGKSFE
                     SEREILYQVLFDMRQDVTELKKLVHEIMSERGAVASNVGTFYAPAPVVTPPPSVPALIH
                     PVKPNCPDDDDIQDTEEYVEESLSLDEVEKEMIRKALEKHHGKRKSAAKDLNISERTLY
                     RKIKEYGLE"
     gene            complement(14834..15931)
                     /gene="pdxA"
                     /locus_tag="K5L50_RS10725"
     CDS             complement(14834..15931)
                     /EC_number="1.1.1.262"
                     /GO_function="GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /GO_process="GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pdxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289667.1"
                     /locus_tag="K5L50_RS10725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="WP_005775823.1"
                     /transl_table=11
                     /translation="MEDNKIKIGITQGDINGVGYEVILKTFADPVMLELCTPVIYGSPK
                     VAAYHRKSLDLPTNFSIVNTAAEAAHNRLSVVNCTDDEVKVEFSKPDPEAGKAALGALE
                     KAIEEFREGLIDVIVTAPINKHTIQSEGFAFPGHTEYIEERLGDGSKSLMILMKEDFRV
                     ALVTGHIPVREIASSITKELIQEKLVIFNRSLKQDFGIGAPRIAVLALNPHAGDDGLLG
                     TEEQEIISPAIQEMAAKGILCYGPYPADGFMGSGNFTHFDGVLAMYHDQGLAPFKALAM
                     DEGVNYTAGLPVIRTSPAHGTAYDIAGKGVACEDSFRQAIYVAIDVFRNRQREKAAHAN
                     PLRKQYYEKRDDSDKLKLDTVDDDI"
     misc_feature    15890..15903
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            complement(15936..16982)
                     /locus_tag="K5L50_RS10730"
     CDS             complement(15936..16982)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785463.1"
                     /locus_tag="K5L50_RS10730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4837 family protein"
                     /protein_id="WP_005775822.1"
                     /transl_table=11
                     /translation="MKKYLFYLSMALVAVVLFSCKGGKKSVFTPTSSGRAYEILVVVDN
                     PVWERPAGRALYNVLDTDVPGLPQSERSFRIMSTSANNFDAILKLVRNIIIVDIQDIYS
                     QPKFKYAKDVYASPQMILTIQAPDEATFEKFVNENKQAIIDFFTRAEMNRQISLLEKNH
                     NDFISKKVNSMFDSDIWLPAELGSSKTGEDFFWAGTNTASADQNFVIYSYPYTDKDTFT
                     KEFFIHKRDSVMKANIPGAKEGMYMSTDSTTVEVRPIDIHGDYTMEARGLWRMKGDFMG
                     GPFVSHTRLDKANHRIVTVEVFIYSPDKLKRNLMRMLEASLYTLQLPTEKKQEQIPMGI
                     EQEEKTNK"
     misc_feature    complement(16978..16991)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 20.8"
                     /tool="antismash"
     gene            complement(17058..18092)
                     /gene="rlmN"
                     /locus_tag="K5L50_RS10735"
     CDS             complement(17058..18092)
                     /EC_number="2.1.1.192"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0030488 - tRNA methylation [Evidence IEA]"
                     /GO_process="GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rlmN"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768025.1"
                     /locus_tag="K5L50_RS10735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="WP_022012997.1"
                     /sec_met_domain="PF04055 (E-value: 3.5e-17, bitscore: 57.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPKYPLLGMTLTELQSVTKDLGMPAFAAKQIASWLYDKKVTSIDE
                     MTNLSLKHRELLKGEYDLGVVAPVDAMRSVDGTVKYLYRVGENHFVEAVYIPDEDRATL
                     CVSSQVGCKMNCKFCMTGKQGFTASLTANQILNQIAALPERDKLTNVVMMGMGEPLDNL
                     DEVLKALHILTASYGYGWSPKRITLSSVGLRKGLQRFIEESECHLAISLHSPFPSQRSE
                     LMPAERAFSIKEMVDLLKNYDFSKQRRLSFEYIVFKGVNDSLIYAKELVKLLRGLDCRM
                     NLIRFHAIPGVDLEGADMETMMAFRDYLTSHGLFTTIRASRGEDIFAACGMLSTAKQEE
                     SDKN"
     misc_feature    18066..18083
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     misc_feature    complement(18242..18256)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.55"
                     /tool="antismash"
     misc_feature    18549..18564
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.55"
                     /tool="antismash"
     misc_feature    complement(19276..19289)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.79"
                     /tool="antismash"
ORIGIN
        1 tttggtttcg caaacaaagt tacagattcc acataaacag gcaataccta aaagtaggga
       61 atttattcga tagaagcaca gcatgaattt cgtttctatt tcataaatgt tacataaaca
      121 ttacaacata actctttcat tatcaatatc tattaataat aaatattcat atattcagta
      181 acaggtctgt aatacgaggg acctattttt gcgccagaaa acaacaagca gatgttatgg
      241 acaatgtaca acaagtaaaa actaattcac aaaagaaact ttccgatttc cttttcattc
      301 tttgggcagg tggtgctgca ttgctctcct actcactggt ttatgcacta agaaaacctt
      361 atacggctgc cggattcgat ggacttgaga catttggaat ggattataaa gtagtggtca
      421 ctatcgcaca gatattagga tacgtacttt ccaagttcat tgggatcaaa cttatttcag
      481 agttgaaacg ggaaaaccgg atgaaattca tcctgatctc cattgtactg gcagaagctt
      541 cgttgatact gttcggacta ctacctgcac cttataatat aggagctatg tttctgaacg
      601 gtctttcatt gggatgtatg tggggaatca tttttagttt tatagaagga cgccgcatga
      661 cggacatact cgccagcctg ttgggtgtca gtatggttat cagctcggga acagctaagt
      721 ccgccggctt gtttgttatg gacactttaa acataagtga attctggatg ccggctctga
      781 tcggaggtat tgctttacct atactcgccc tattgggtta ttctctgaac cggctcccac
      841 aacccacggc cgaagatatc gccatgaaat cgaaacggga gacactgaac ggcaaacagc
      901 gttgggatat cttcaagaat tttatgcctt ttctcaccct gctcttcatt gccaatgtag
      961 tattgacaat cttaagggat atcaaagaag acttcctggt gaagattatc gatgtatccc
     1021 aatactcttc gtggatgttt gctcaggtag acagcgtagt aactatcatt atccttgtca
     1081 ttttcgggtt gatggttttt gtacgaagca acttgaaagc attatcgata ttactgggat
     1141 tgatcatcat cagcatggta gtgatggtag tggtttcatt tggctacgaa caattgcaat
     1201 taaacgctat tgtctggtta ttcattcaga gcctctgtct ttatctggct tttctcactt
     1261 ttcagaccat tttcttcgac cgattcatcg catgctttaa aatccggggt aatgtaggtt
     1321 tctttattgc catgaatgat tttctcggtt atacgggtac ggttatcgta ttggctgtca
     1381 aagaattctt ttcacctgac attaactgga ctactttcta caacctgatg gccggctatg
     1441 taggagtgat ttgttttgtc gctttcgtat gttcatttat ctatctgcat caacgttatc
     1501 gtagagagaa ctacggaaaa acaggcgtat tcagaaaaaa agaagaagaa aaagaagttc
     1561 ctgatttcgt atattaattg ctatctttgt gggtcaaaaa acttaaaata aaagttcacc
     1621 atgaagattt ttccaagtag cagcatcaaa aaactggatg cttacaccat agaacatgaa
     1681 ccgattgcat cgattgacct gatggaacgg gcagcgcagg cactgacaaa agccatcact
     1741 gaacgttggg acatcacaac tcctgtcacg gtatttgcgg gaccgggtaa taacggcgga
     1801 gatgcccttg ccgtggcccg catgctggcg gaaaaggaat ataaggttga agcctatctg
     1861 tttaacccga aaggagaact gtcagccgac tgtcagacca acaaagagtt ggtggaaatg
     1921 atggataatg tgaagttcag tgaagtaagc acacaatttg tacctcctac cctgacaatg
     1981 gatcatttgg tagtggacgg actttttggt tcgggactca ataaaccgtt aagcggcggt
     2041 ttcgcggctg tagtgaaata tatcaatgca tcgcctgcta cagtggttgc tatcgacatc
     2101 ccttcgggat tgatgggaga agaaaacaca tttaacgtaa aagccaatat catccgtgcc
     2161 catctgacac tgagcctgca attgcccaaa ctggcttttc tcttcgcaga gaatgccgaa
     2221 ttcgttggtg aatggaaatt gctggatatc aacctcagcc atgaagccat cgaagaaaca
     2281 gaaagcaatt atgccttatt ggaagcagaa gagatacatg ctttgatcaa accccgcagc
     2341 cccttttcac acaaaggaaa tttcggacac gccttgctga ttgccggatc gtatggtatg
     2401 gcaggggctt cagtgctggc ggcccgtgcc tgtatgcgtt cgggggtagg attgctgact
     2461 gtacacgcac ccatgcgtaa caatgacatc ctacagattt ccgtcccgga agcaattgtc
     2521 gaactcgatg ccagtgattc ctgctttgct tttcccacag atacagatga ctatcaggcg
     2581 gtaggtatcg gtccgggaat cggacgctca gaagaaacag aagccgcatt gctcgagcaa
     2641 ctcagtggat gccagactcc tttggtgctg gatgccgatg cactgaatat attagccaac
     2701 caccgacatg cactgaccac gctccccaaa ggctccatcc tgacccctca ccccaaggag
     2761 ctggaacgta tggtgggcaa atgccagaat tcgtacgagc gactgatgaa agcttgtgaa
     2821 ctggctcgta ccgctaaagt acatattata ttaaaaggag cttattcagc aattatcacc
     2881 ccttcgggca aatgttattt caactccaca ggtaatccgg gcatggcgac agccggaagt
     2941 ggagatgtgt tgacgggtgt cgtactggct ttactgacac agggataccc ggctgaagaa
     3001 gcagctaaaa taggtactta cgtacacggt ctggccggcg atttcgcacg caaaaagcag
     3061 ggcgttatcg gtatgacagc cggagatatt gtcaattatt tgccaatggc ctggcgtctg
     3121 gtaagcgaat aaaggttaac aacttatgat tttccaaata acttcattaa ctttgttttt
     3181 taaaccagtt aaaataaacc ggaaaatgaa aaagctaatt atactgacag gactgttact
     3241 ttctgcctcg gcatatgccc agacagaagt tacagcggga gttacccggg gaaaagatta
     3301 cggggtgacc tatgcacttc ctaaaacagc aatcgacatt gaagtcaaag ccaataaagt
     3361 gacatacact ccgggagaat tcagcaaata tgccgaccgt tatctgcggc tgaccgatgt
     3421 gtcgggagag cctcaggaat attgggaact ggtcagcgtc aaagccaaat ctgtcggtat
     3481 ccccgatagt gaccatacct atttcgtcaa actgaaggac aaaacagtgg ctccgttgat
     3541 agagttgacc gaagacggta ttgttaaatc gatcaatgta ccgttaagtc cgaagaagtc
     3601 tgcacctata caaccagcca ctacgctgag aaaaaaagta aatccacgcg attttctgac
     3661 agaagagatt ctgatggcgg gttctacagc taaaatggct gaattggtcg ccaaagaaat
     3721 ctacaacatc cgcgaaagca aaaacgcctt agtacgcggc caggcagaca acatgcctaa
     3781 agacggagaa caactgaaga taatgcttgc caacctggaa gaacaggaag ctgccatgac
     3841 cgaaatgttc tcaggtacac tgaataaaga cgagcggata tttactatcc gcctgaatcc
     3901 ggaaaaagag atggataacg aagtggcctt ccgcttttcc aggaaactgg gtatagttgc
     3961 caataatgat ctggcaggag aaccggttta cattactctg aaaaatctga aaacagtcaa
     4021 tgtaccggaa gacgatggca aaaagaaagt agacggcata gcttataacg tacccggtaa
     4081 ggcgcaagtg acgctcatag aaggaaaaaa agaatggttt aacggtgaac ttcccgttac
     4141 acagttcgga acaattgaat atctggctcc ggctcttttc aataaaaaat cgaccgtaca
     4201 agttactttc aatccggcta caggaggatt gattaaagta gatagggaag aaggagaata
     4261 aagcataaaa ctgacacaac ctcatcagtt ccagtttttt catatgcttt tccatttcaa
     4321 actacaatta tgcgtcagat ggccggtgaa tacgtccact gcattataaa gggctaaagg
     4381 atgaaaaatg ttcaccctgc accggaaaaa tcttcggtat caaaacaggt attccaaccg
     4441 ttgatattaa aagctataaa aataaaatgg aacaaatatg gaggatatga agaagttttt
     4501 accggaagcg gatctctcaa aactaaggac tatacttgat ataaagaatt cttataactg
     4561 cgggacgctc tctcttgaag atgcccgtaa acttcttaag gagaaagtgg tagtcttgaa
     4621 actatatgaa ataacgtttg ctgaacagga actgaaggga ggggaggaga atgagtgccg
     4681 caaggaggat attcagcgca tgctggaact ttttaacggc ataatggaca cttcacgccc
     4741 cgatttaccc ccggagtatt ctgtcatgta ttattatcgt ggaaacgacg agttacgtaa
     4801 aatcctgccc tcaattgagg ggctggtccc ccctccttac ccgacaaatc cggaacagaa
     4861 ccagtacccc gtctaccttg ttgaaggact tattggcggc atatcctctt ttgaaagaca
     4921 ggatggagga gatcagcccc aagttcaaac tgctcaggtc ccctttggcg cgggttatac
     4981 tacccagagc caccatcagc acgataaccg ataaactagt atcccggcgg acacactagt
     5041 tgaatccctg aggtccagga tcgagaaact tccactattg cagacatttt tttcacagag
     5101 ctatatggta cgattccccg taaccctttc tccgaaccag gattacgggg attgttatcc
     5161 ttatacgggg cacttatcgt ctggggactt tcatctcgtc cccccagtgc gcctttgcct
     5221 cccgttcctc acgctcacgt atttcctttg ccttgcgcgc atatacgatg atacgcagtg
     5281 actggtcata ggtgaaatag ttccacatcc agttcaacag cacgttgacc ttgttcctca
     5341 ctcccaagat ggagcgcaag tgaacaacca gccataacac ccatgccatc aatccttgtg
     5401 tcctcaccct attgaattcc gctaccgcac ggtttcttcc tacagtggcc atggagccca
     5461 gatccctgta tccgaacggt ctcatcggct ttttccttgc cattcttttt aggttggcgg
     5521 ccagtaaccg cccctgttgt atggctactt gggccagctg cggatgccct tccggatact
     5581 tcctgtcagc agtcattatg cactggtctc cgattgcaaa aacattctcg accccctcta
     5641 ctcggttgaa tgcgtccact tttatgcgtc cgccacgtcc tatgcattct gccggcatgt
     5701 tgcccaccgg gactgccttt acaccgctta cccagatgaa agagcgtgtg ggtatggcca
     5761 ttccgtcctc cagtatcact ttgtggtccc tatagtcaac tacacgcttg tgcaagcata
     5821 tgttaacccc catattgcgg agaaactgct cggcccttgc ggaggattcc gcggacatgc
     5881 ccgaaagtag cctacccgca gcctctatca aaaatatatt gattctgctg ctgtccatat
     5941 ccggatagtc agccggaatt acatatttcc tcatctccga gagtgcccct gcaatttcaa
     6001 ctccggttgc tcccccaccc acaatgacta tgttctgcaa ctcctgtttc tcctgttcgg
     6061 tggagcatgt gatacttctt tccagattgg acagcaacgc attcctgagt cccatcgctt
     6121 cggaaaccgt cttcatgggc atggcctctt cctcgatccg gctgttcccg aaaaaattgc
     6181 tggttgtgcc taaggccagt accaggtagt catagtcgat ttttccgata gatgtctgaa
     6241 ttattttctt ttcgggataa atcccccgca attcggccat tctgaaataa aagttcttcc
     6301 ttttcctgaa tatcctcctg aaagggaatg atatcgaact cggctccaat ccggaggatg
     6361 cgatctggta aatcagcgga gggaactggt ggtaattgtt cctgtcaagt agaatgacct
     6421 ggtaatcaga cttccggagt tctccagcta gtttcaaacc tccgaatcct ccacctgcga
     6481 taataattct tttctggtta tgatttcttg caatattaaa actcatttat ctatatatgt
     6541 taatgtttaa aagattcatt aatctgtgtt ctgcatgaag tatatagact tggactttca
     6601 gttgcctatc atcaggataa cggcactttt tcaaaaaaga gcggaacata tggatatgat
     6661 ccgtgacaat atatcttcgt cattatacca tcctagttac aaaaaagaag ctgcaatgag
     6721 ataaactgcc gccgccaata atccgcttac cggaatggtc agcacccagg cagtcatcag
     6781 atttttggta actccccagc gaaccgctga cagccgctta gtggccccta ccccgatgat
     6841 agctcccgta atagtatgtg tcgtactcac tgggatcttc agatattccg ttagatacag
     6901 cgtaaaggca ccggctgtct ctgctatgac tccctccaaa ggggttacct tggtaatttt
     6961 ggttcccatc gtctttacaa ttttccatcc accggacatc gtacccagtg agatggccgt
     7021 aaaacaagag aatgccaccc agtccggcaa atcatcaata ctgtttatcc ccattcccaa
     7081 actttcagaa tgtcccgcta tcatggcagc ggcaataata cccatcactt tctgggaatc
     7141 gttcaatccg tgtccgatac tgaacatagc tgaagaaaca agctgcagtt tcttgaacca
     7201 gacctcggca gtatggggat gcgcccgttt acagatataa aacaccagca aagtaacccc
     7261 gaatgcaatg accatgccta tcagaggagc caaaaagatg aaaaccgcaa tcttcagtat
     7321 gatattcaac tgaatggctt caaaaccgtg agccataatg gctgctcctg caaaaccgcc
     7381 tataagggta tgcgatgatg acgaaggaat acctttccac caggtaaaca ggctccaaac
     7441 aatggcagct atcacccctg ccaggataac gggcaatgtg atgtatggct ctactaccgt
     7501 tttcgatacg gtattggcta taccaaagcc cccaatgacg tatttggcta taaagaaagc
     7561 taaaaagtta aagaatgcag cccatatcac ggcctgaaaa ggagtcagca ctttagtaga
     7621 tacaatagtg gctattgaat tcgctgcatc gtgaaaaccg ttgatgtaat caaatatcag
     7681 agccaatacg atgataatca ctaatagttc catgtgttat gcctgtttat gcgtatttca
     7741 caatcaaact tcttaatatc ttgcccacat gctccgcagc atcggtggtc ttttccaatt
     7801 cgtacacaat ctccttaatc ttgatgagtt cgatacagtc cttctcctcc tcaaaaagtt
     7861 tggtgataaa taattcgtac acatcatccg catggttttc aatatcatgc attttcgaac
     7921 aatatccacg caaagtattt ggattctgac ggaaagtctc gagctcgtcc attgccttga
     7981 caatgtattc cgcctcctgc tgaatcatac ggctcagttc cttcccgctt tcggagatag
     8041 gatgcggatt atagatggca atacgttttg cgcaactgtt tataccgtca atcacatcat
     8101 caatacctga agccagatca tggatgtcct cacggtcaaa tggcgtaata aaagtggttc
     8161 ctagttcgtc cagaatccga tgggtaagct tatccccctc acgttccata tccttgatcc
     8221 gcttatagaa aatggccctt ttttctggtg aatcatgctt catcccttcc actaaaagtt
     8281 ccgatgaagc agtcagtatt tcagacaact gtttcagcaa aggaaaaaac ttgggttctt
     8341 tgggtgtaaa tctgctaaaa aaagaatttt tcataaatgt gtgtatttaa aattatttcg
     8401 tttgtttacg agttttcaga cgggcacgta taacctcgat cacagcaggt aatactgata
     8461 ctactatgat agcgacaatc agcagtttaa ggtttttctg tacgaaagga aggtctccaa
     8521 aaaaatatcc cgcgtagcag aaaatggcaa cccacaacac tccgcccgtc agattgtaca
     8581 gcatgaaata gtaatagtgc attttaccca ttcccgccac aaatggggca aatgtacgga
     8641 caatgggcac aaagcgtgca aggataatag tcttaccgcc atacttttta taaaactcat
     8701 gtgttttctc aagatggctc tgcttgaata tcctggaatc gggattacgg aacagcttcc
     8761 gcccaaagaa ataccctatc atataattgc aagaatcccc caaaacagca gcagcaaata
     8821 aaattaagac cagcacattc acctcgacaa gcatatcggg tagtgcagca atggcacccg
     8881 ccacgaacag cagggagtca cccggcaaga acggtgtaac caccagaccg gtttcgcaga
     8941 aaataattaa gaataagatg acatacgccc aggtatggta ttcttgtacg atgtcaatca
     9001 tatattgatc gacatgaaga atgaaatcca ataaaaaatc cattttgtat catttatcag
     9061 tgcttaaata agaagaaaga tacaaagaga taaagatccc tttgaaaaga gaaaaaaagc
     9121 actaaataag aatgcgcctc agtgtataga cataatatct ataagagaag ttgaaaggcc
     9181 ttatactgaa tgaaaaggag atcgtttttt cttcaggaag taaaaccttc agagatttca
     9241 ccattctggc aatgatcaag gctgtattgt ccctcaatat cttggaaggg gagatacaac
     9301 ggaacgaaaa gttatttcct atacagactc tgtccgtcct gtcattgtag gatccacacc
     9361 atattcctgt ttcatggcga tccagatcta actcaaacaa accgtgctgg tttccggaaa
     9421 catgccggat atctatggta tgcgacacct tgtattcctt tatatcatcg gaggctgaca
     9481 ccacctttaa tatgaggaac aatcctaata acatatagaa gatatacttc gccatagcga
     9541 ctgcaaagat agaaagtatt tttaagtgtg ggaaccttgc cggattttta ttttcatctt
     9601 tccggattga atttccgggc ggcttctgtt atttgtcacg ttttatggat tgggcgaaac
     9661 atttcggata ttatagagac ttttcaaaag gcatgatacc gatatcagac gacgagacat
     9721 cttctacatg acaaggatag tctgcgttaa aagttatgaa gtgtctgttt aaacatcatc
     9781 atctgtccgt tcgcaatcac taacgtaaga ttatgctccg gcaagtttta cgaggatgtc
     9841 cttctgatat cctcataccg ctcggaacaa tatctcggta tccataccga caaccttctt
     9901 cggaaatatt ggcggctatt gttatggaaa tggggcatgt gcacttcatt tttggcacac
     9961 cccctttttt attttcttaa attgtgcttt tcaaacaccg tcagttcgta actgctaatt
    10021 tcacaaatcc gtccttctgc aactgggtga tataagcagt aatacacgat tcgtaatttt
    10081 cttcgtgatc aaaatgctct gcaagttctt caataatctg gcgtacagtc cgcttcccgt
    10141 caattaactc ccatacggca gtaccatgtt cttccagccg gacatgaata tccggagaca
    10201 ttcctttggg caataaaaaa cgccgcatcc attcatattt aaaccggggg aaagcaatgg
    10261 taacggtacc atcgcttccc ttttcggctg taatattctc actacggaaa ggaattacat
    10321 ccaaaagatt tattttctct ttcgcgtctg ccatattctg ttatttctta atattcgctt
    10381 cctgcaatcc gtatcccttc ttcttatctt cagctttcag catcaaagcc acaatcagtg
    10441 ccaaaacacc aaaacaagca aagatagaca taggcaaagt ataatcatag gtctgtgcac
    10501 catctacaac gggacctttg caataagtat tcagtaccca tccgatcaac aaaggaacac
    10561 ccatcaatcc ccagttctgt acccagaaaa tcagggcata ggctgtaccc agttgtttct
    10621 ccggaataat tttcggaaca gaaggccaca tggcagaagg caccagtgag aaggcaatac
    10681 ccagaattat cataattaca gtggcaaacc accatacatt aaggatagga agcgcaaaca
    10741 aagtatgtac gccaatcaac ataacggcac cgataatcat taatgtagct cccttaccga
    10801 tacggtcata aagagtacca aacaacggag tcaggaagat ggtccctatc ggtagtaatc
    10861 ctgggatatt tccggccaat ttaggatcta cgttatactt ctgcaccatc aaatcagttg
    10921 catatttaat aaaggggaag acagcggaat agaacaatac acacaataaa gcaatcaacc
    10981 agaatccctt attagtaaca ataagcataa catccttcat acggaacggt tcctccggtt
    11041 cttctccctg agcatctaaa gaagcatcaa gtttcttatc ataaaaggta tagataaaga
    11101 aggcgatagt accgatgcac agcataatca gacacaacaa aataggcatc ggaatattgg
    11161 tatggaaact gccactttca tccgtatagc caaaataatc ggcaatagga acagtaagta
    11221 ccattgccaa agtagtaccg atacgtgcgg ttgccatctc aagtcccatg gctaaagcca
    11281 tctctttgcc cttaaaccat tttacaataa tcttggaaac cgtgatacct gcaatctcta
    11341 cacctacacc aaagatagca tatcctaaag cagccagaaa gacctgagtt ttgaagccca
    11401 taatcaaagc accttcgggg aaagttgtag aaatagcata atatttcagt ccacaaccca
    11461 gcaccatcag tatacaggct cccattccgg tgaaacgaac tcccatctta tcgagaatga
    11521 taccaccaaa aatcaacatc agcaggaata cattaaacca cccgtaagcg ctggtaaaga
    11581 atccataatc caaactatcc caaaggagct ctttttcgag catgggtttc aaaggagaca
    11641 ttacgtcggt aaggaaatag ccgcaaagca tagtaaaagc gaccagagcg agcacactcc
    11701 aacgaagtgt tttggagtca ctcagtttgt ttctcaattg ttctgtcatt gtaatattta
    11761 aactcagaag ttagtaaaag tgttatagcg aacaaaggta actaaatccg ccttgtatgc
    11821 ctaacaaaaa gatacatttt acggctaaaa acaagtgaag ctgcaagttt cggtttgaaa
    11881 ttgcagcttc acttatcgtt tcttaaaaag aatctgttgt tagtttgccg gggttgccgg
    11941 agtttctgtt gcaggtgctt cagccggagc agcagcatcc gtcttcggtg cagtagtacc
    12001 tacaggcagg ttatacgggt tggtctgttc ttcctgctga gcctgttcca tgatgacatc
    12061 ttgcgtttta gaagaagaag tcggaacaac ataagcagta gcaatgctca tcacaaccat
    12121 aaatgcagct aagccccaag ttgccttttc cagaaagtct gtagttttgc gtacacccat
    12181 aatctggtta gatgacgaga acccagaagc aagaccaccg ccttttgagt tctgaatcaa
    12241 cacaatgaag cacatcagta tggctgcaat aaccattaag ataactaata ataagtacat
    12301 tttgttattt tgatttagcg ttaataatca atttctctaa aaatctgatt tggtctgcaa
    12361 agtaagcatt tttttttgga tatttcaaac ttaatttttt aataatttca agtgccttgg
    12421 aatatcggtg ctgtttgaca tatattttgg ctaatgtttc ggtaaaacag ctatcatctt
    12481 catcttcttc atgatgcgcc tccccctctt cttccacggg aagcgtttca ttgttagctg
    12541 cgggctgcag acgaatggat gtttctccct cacttctctc gataaagcca tcgatcaatt
    12601 catgcccacg aagtttcgga atctccgact gttccggagc ggaatcttct tcatcttcct
    12661 gtaacagata agtagtataa tccgttgaat agtccagact tgtctgcccg gaaacctctt
    12721 caggcacagt ggccaaaaaa gcatcgatca gggagagtgt acgatccaga ccgggctctt
    12781 cctccaacac ttctgcttcg gaaggcatat tctttttacg gggcttcaaa atatacctct
    12841 caccttcaat cagataaaac agcttccggc gatctgccac atataatata gctttacgaa
    12901 gctccgcacc gaaagagata tcgtgtaaca gataaagatt tttaagatag agtaaacgca
    12961 gcgactggaa ataaggatag cgtgtgacaa gtgtccgcaa ctcgtacaaa gtatctttat
    13021 tcagcgtttc aggatgctga atccattgtt gtaaattagc agaaatcatc ccaattacca
    13081 atttgctacc gttgcattaa atatttgatc tgtaatctct ttggtcatct ccgcaatcag
    13141 tccgtcctga acagctgtca gtaactctct tgagtcgtag acacggaaag ccgagaattg
    13201 ctgttcgaag tcctgctcat ggttcgtatt attcacaaaa cgaacattta ccgtaatcgt
    13261 cagcttcgtt tcagaagagt atccatcggc cgaaacagct tggttatact ggttgtatcc
    13321 ggtgatttct ccatcaatct gaaggtcggc attattattc accagcttca ggcgagtctg
    13381 gcgaaggaag atatctttca gatcttcatt gaatttagta cctaacggtg cataaacata
    13441 gtccgacttt ataggaaagt cggctataga aatagtcttt accttatcgt aattgatatt
    13501 accaccatta aatttataag aaacagtaca cgagatgccc actaccagcg tgacaaccag
    13561 tagtgagatt cgcattatct ttttattcca atccatattc ttttattttt cggtaaaggg
    13621 tacgctcaga gatattgagg tcctttgccg cactttttcg tttgccatga tgtttctcaa
    13681 gggctttacg tatcatttct ttctctacct cgtccagcga aagagactct tcaacatact
    13741 cttcggtatc ctgtatgtca tcgtcatcgg gacaattagg cttgaccgga tggatcaggg
    13801 caggtacaga aggcggaggc gtcacaacag gagccggggc ataaaacgta ccaacattgg
    13861 aagctacagc tccgcgttcg gacatgattt cgtgcacaag ctttttcagt tcggtcacat
    13921 cctgccgcat atcaaagaga acctgatata aaatttcacg ttcactttca aaactcttcc
    13981 cggcctttac accgaacaaa gccggcagtc gttgtgtatt ctgggcaggc agataggtcc
    14041 gcaaaataga agcattgatt tcacgatttg tctcaatgat ggagatctgt tccgtgatat
    14101 tcttcaattg gcgcacatta cccggccatg aatatgacaa caaaacctgt ttggcatctt
    14161 cggtcagttg tatcgcaggc atacgatatt tctccgcaaa atcacttgca aacttgcgga
    14221 acagcaacag cacgtcttct ccacgttccc gcaaaggagg aatctggatg ggtaccgtat
    14281 tgagacggta atacaaatcc tcacgaaaac gcccttcagc aatggcttgt gtcagattga
    14341 cattagtagc agccacaata cggacatctg ttttctgcac tttggaagaa cctactttta
    14401 taaactctcc actttcaagc acacgaagca aacgcgcctg agtaggcaaa ggcaactccc
    14461 cgacttcatc cagaaagata gtccctccgt cggcctcgcc aaaatatcct ttccgctcac
    14521 caatagcacc tgtaaaagcc cctttttcat gcccaaatag ttccgaatca atggtcccct
    14581 caggaatagc accacagttc acggcaatat actggccatg tttacgacgg ctatactgat
    14641 gaataatctg aggaaagctt tccttgccga caccactctc tccggttatt agcacggaca
    14701 aatcggtagg cgctacctgt atggcaacat ctatcgcacg cgtcaaggct tcagtgttac
    14761 caataatacc gaaccttagt tttacttgtt gtatttccgc ttttgtcata ttcgcactaa
    14821 actcccttcc tgattaaata tcgtcatcta ctgtatcgag cttcagttta tcactgtcgt
    14881 ctcttttttc gtaatattgt ttacgcaacg gattggcatg tgctgccttt tcacgctggc
    14941 ggttacggaa tacatcaatc gctacataaa tggcttgccg gaatgaatct tcacaagcaa
    15001 ccccttttcc tgcaatatca taggctgtac cgtgcgctgg agaagtgcgt atcacaggca
    15061 gacctgccgt ataattcaca ccttcatcca tggccaatgc cttgaaaggt gccagtccct
    15121 gatcatgata catagcgagc actccgtcaa aatgggtaaa attacccgat cccataaagc
    15181 cgtcagccgg ataaggacca taacataaga ttcctttagc ggccatctcc tggatagccg
    15241 gagaaatgat ctcctgttct tccgtaccga gtaacccgtc atctccggca tgtggattca
    15301 gtgccaacac tgcaatgcga ggtgcgccga ttccaaaatc ctgtttcaac gaacggttga
    15361 aaatgacaag tttctcctga atcagctctt tggttatcga agaagctatc tcccgaaccg
    15421 gaatatgacc ggtcaccaaa gccacacgga aatcctcctt catcaggatc atcagagatt
    15481 tcgaaccgtc tcccaaacgc tcttcaatat attcagtatg tccggggaaa gcgaatcctt
    15541 cggactgaat tgtatgctta ttaataggag ctgtcacaat gacatcaatc aacccttccc
    15601 tgaactcctc tatcgctttt tcaagtgccc ctaaagctgc tttaccggcc tccggatcgg
    15661 gttttgaaaa ttcgactttc acttcgtcat ccgtacaatt gactacactc aaccgattgt
    15721 gtgcagcttc tgcagcagta ttgacaatac tgaagttagt aggcagatcg agtgatttac
    15781 ggtgatatgc agccaccttc ggcgaaccgt aaatgaccgg tgtacaaagt tccaacatga
    15841 cggggtcggc aaaagtcttc aagattactt cgtatcctac cccgtttatg tctccctgag
    15901 tgataccaat ttttattttg ttatcttcca ttttgttatt tgttagtttt ctcctcctgc
    15961 tctatgccca tcggaatctg ctcctgcttc ttctcggtag gaagttgcaa agtatacaga
    16021 gatgcttcca acatgcgcat caggttacgt ttcaatttat ctggtgagta aataaatacc
    16081 tctacagtga caatacgatg gttagccttg tccagacgag tgtgcgaaac aaacggacca
    16141 cccatgaagt cacccttcat acgccaaagc ccacgtgctt ccattgtata gtcgccatga
    16201 atatcgattg gacgaacctc gactgtagta gagtctgtcg acatatacat accttcttta
    16261 gctcccggaa tattagcttt catcacagaa tcgcgtttat ggatgaaaaa ctctttcgta
    16321 aacgtatcct tatcggtata cggataagag tagatcacaa aattctgatc agcggaagct
    16381 gtattcgtac ctgcccagaa gaaatcctct ccagtctttg aactgcccaa ctcagccgga
    16441 agccagatgt cgctatcgaa catactgtta accttcttcg aaatgaaatc attgtggttt
    16501 ttctctaaca gggaaatctg gcgattcatc tcggcacggg taaagaagtc gataattgct
    16561 tgcttgtttt cattcacaaa cttttcaaat gtggcttcgt ccggagcctg aatagtcaga
    16621 atcatctggg gagatgcata tacatccttg gcatacttaa atttaggctg actatagatg
    16681 tcctgtatat ctacgataat aatattacgt accagcttca ggatagcatc gaaattgttc
    16741 gcagaagtag acatgattct gaacgaacgt tccgattgcg gaagtccggg tacatctgta
    16801 tcaagcacat tgtacaaagc tctaccggca ggacgctccc acacaggatt atcaacgacc
    16861 accaggattt cgtaagcacg tccgctggaa gttggagtaa acacactttt cttgccgcct
    16921 ttacaagaaa acaatactac tgctacaaga gccatactga ggtaaaacag gtactttttc
    16981 atatgcatca attttaatgt tttcacaaaa ctacgaaatc accgtataca tatagtctac
    17041 gaagctgata atttatatta attcttgtca ctctcttcct gcttggcagt cgataacata
    17101 ccacaggcag caaaaatatc ttctcccctt gaagcgcgaa tcgtagtaaa caatccgtgc
    17161 gacgtcagat aatcacgaaa tgccatcata gtctccatat cggcaccttc aagatccact
    17221 ccgggaatgg catgaaagcg aatcagattc atccggcaat caagcccacg aagtaacttt
    17281 accagctctt tggcataaat caacgaatca ttgacacctt taaaaacaat gtattcaaac
    17341 gaaagtctgc gttgtttact aaaatcatag ttttttaaca gatcgaccat ctcttttatc
    17401 gagaaggctc tttcggcagg catcaactct gaacgctgtg aaggaaaggg agaatgcagg
    17461 ctgatggcca gatggcactc gctttcttca ataaaacgct gtagtccctt ccgtaacccc
    17521 accgacgaca aagttatacg ttttggactc caaccgtatc catacgaagc tgtcaggata
    17581 tgcaatgctt ttaaaacttc atccaagttg tcaagcggtt cacccatccc catcataacg
    17641 acattggtca acttgtcccg ctccggcaat gccgcgatct gatttaaaat ctgattggca
    17701 gtcagacttg ctgtaaatcc ttgcttgccc gtcatgcaaa atttgcagtt cattttacac
    17761 cccacttggg aagacacaca cagtgtcgcc cggtcttcat caggaatata taccgcttca
    17821 acaaaatggt tctctcccac gcgataaaga tacttcaccg taccatctac ggaacgcata
    17881 gcatccacgg gtgcgactac tcccaaatcg tactcgcctt tcagcaattc tctatgcttc
    17941 aacgacaaat tagtcatttc gtcaatagaa gtcacttttt tatcgtacaa ccaggaagct
    18001 atctgtttgg ccgcaaaagc cggcatcccc aagtctttgg taacagattg caattcggta
    18061 agagtcattc ctaaaagcgg atatttgggc atatttacta caaatattta tttattcatg
    18121 caaatgtaag gaataaaagt tgatttttga aaagagaaag gacaaaagtt aataagaaga
    18181 aggaaagaga gaacaaaaag aaaaggccat tccacaagga atgacctttt atctataaaa
    18241 ctattttctg aaaaaaacga gtcttgtttt tagaagaaca ggtaacgctt gtcttttaca
    18301 tcacctttca gataaagatc gttcatgaaa cgggcagcca gacgggcatg catattttcc
    18361 agagtaacct cctctgcctt ggcatcgaat gtttcattca acttatcttt ggcatatacc
    18421 tgcaatacaa atacaccgcc attacctttg ataggagcgc tcagtttatt aatatcggct
    18481 accgatgcat aagcacctac cagcggttcg ctactacgca aagcagctac ataggcagga
    18541 gccgcaaatg ttacgtgttt cacagaatcg ctcactgcat tggccattga tttatactga
    18601 tcgaaagaag ttgcattgac agccttcatg tcagccataa ttttttcagc cttcttatca
    18661 cgaataattt ctgctcttaa ctgatcctga acctgagcca aaggacgata accttcttcg
    18721 atcacgttaa ccaggccgac aaccaacata cgatcgctct caccacattc atacaaaccg
    18781 gaaacttcac cgggttttgc agcaaatgtc catttcagag cttctttggt tcctctgata
    18841 ccaccgattc cgtgttccga gctatacaaa tcatttcttt caagcaattt ataacctgct
    18901 tcttcagcat tagccgccat cttttctacc gtcggattgg ctgcgataaa ctgactgaag
    18961 tcattatatg ccttattata agtttctttg ctgaagtcaa ctgcgcgttt gattacggca
    19021 actttatatt tatctttcac agctttcttg tcagtaacct gcaaaatgat gttaccctgt
    19081 cccaatgcta cattagagag ttcgttcact cccaggttgt tgatcgtggt gataaacttc
    19141 aggttgtcac catcaatctg tgcattttcg tagttagcag aagaaatcca gttggattca
    19201 cctgtctgtc catacttttt agccaaagcg gtgaagtcgg ccccaccttt aattgctgta
    19261 taaatactat cagccaaaac ttttgtttta gcagcatctt cagcataaac ctgaatctga
    19321 cggaactgta cagaatcggc agcagccact ttagacaaaa ccttgaaaga gttaatggta
    19381 ttatcaccgg cattataata aggaccatat atctgtccca ttgaagctga atccatccgt
    19441 gcaactacat ctgacggaaa agctctttta gtataataca aatcaacgta aggatattca
    19501 gatcctgtcg aacggatgaa agtagtataa tcaccattag cagtagccaa ctgatttgta
    19561 taatcagtca cttcctgctg aatagcggct ttatcttccg cactggcagt cacctgtacg
    19621 tcgatatatt tgatattgcg tgtttcaaca tattgtttga actgttcttt cttcttatta
    19681 tagagatctt tcagctctga ctctttcact gtaatggtag aatcaacaat tgaagaataa
    19741 ggcacagcag ccaacagtac atccgattgg ttcactcttg catcgaaagc atcttgtgct
    19801 tcaaccgggt tagagaaaag agcctttgtc accaatgcct gatatttttc agccagacgg
    19861 ctctgaatca gagttttttc aacgaatgac caaagtttgt gcatgccttc atagtattct
    19921 acatattgag acggcatctt agttttgtcc attttagaat agtcagacaa aaatttcttc
    19981 aacatatctt tatcgaaagc acccgtttgc ggattgcgga acggagtctg ttgcagcaac
    20041 ggattcacac cttcgttgat gatagactga atttcagcct ttgaaactgt aatccccagc
    20101 ttcttcgctt ctttttcaat cagtttattg ttcacgtagc tacgccatac ttcgtctttc
    20161 acctgattgg tttgttcatc gttcaatgaa ctcattccgc tggagaactt gatgacttca
    20221 gtatattctt ctaccatatt ctggtagtcc tgagcagaaa gagtttctcc gttgacttcg
    20281 cctacatcat gcgactggtg tggctggaga actttccagg catcgcccgc aatgaaagca
    20341 aacaaagcca aacc
//
