LOCUS       NZ_JAFKPO010000001     13560 bp    DNA     linear   CON 15-DEC-2024
DEFINITION  Bacteroides fragilis strain FSHXHZ1E1 Scaffold1, whole genome
            shotgun sequence.
ACCESSION   NZ_JAFKPO010000001
VERSION     NZ_JAFKPO010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Pseudomonadati; Bacteroidota; Bacteroidia; Bacteroidales;
            Bacteroidaceae; Bacteroides.
REFERENCE   1  (bases 1 to 1144155)
  AUTHORS   Wang,C., Li,S., Hong,K., Yu,L., Tian,F., Zhao,J., Zhang,H., Chen,W.
            and Zhai,Q.
  TITLE     The roles of different Bacteroides fragilis strains in protecting
            against DSS-induced ulcerative colitis and related functional genes
  JOURNAL   Food Funct (2021) In press
   PUBMED   34308455
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1144155)
  AUTHORS   Wang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2021) School of Food Science and Technology,
            Jiangnan University, 1800th Lihu Avenue, Wuxi, Jiangsu 214122, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 2.3 OR MAY-2011
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019633825.1-RS_2024_12_15
            Annotation Date                   :: 12/15/2024 11:10:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,946
            CDSs (total)                      :: 3,876
            Genes (coding)                    :: 3,807
            CDSs (with protein)               :: 3,807
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 26 of 69
            Pseudo Genes (incomplete)         :: 50 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 19 of 69
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:57:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 184159
            Orig. end                         :: 197719
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAFKPO010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13560
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..13560
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13560
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8560](+)"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8560
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     misc_feature    1395..1408
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 21.31"
                     /tool="antismash"
     misc_feature    complement(2601..2615)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.15"
                     /tool="antismash"
     misc_feature    3151..3165
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.3"
                     /tool="antismash"
     gene            3971..4489
                     /gene="upeY"
                     /locus_tag="K5L50_RS00760"
     CDS             3971..4489
                     /codon_start=1
                     /gene="upeY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778177.1"
                     /locus_tag="K5L50_RS00760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="capsular polysaccharide transcription
                     antiterminator UpeY"
                     /protein_id="WP_005778177.1"
                     /transl_table=11
                     /translation="MSQQQEYWFAARTRKDQELTTRDALEKIGVEYFLPTQFVIRQLKY
                     RRRRVEVPVIRNLIFVHATKEFACAIANEYGVRLFYMRDFDTKSMLIVPDKQMKDFMFV
                     MNLDPAAVILNDDCFAVGTKVQVIKGDFCGVEGELASLSNRTYVTIRIRGVLSASVKVP
                     KSYLRILAP"
     gene            4525..4998
                     /locus_tag="K5L50_RS00765"
     CDS             4525..4998
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292448.1"
                     /locus_tag="K5L50_RS00765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="UpxZ family transcription anti-terminator
                     antagonist"
                     /protein_id="WP_259444526.1"
                     /transl_table=11
                     /translation="MTQIHELQHVAHELLYLGADGSPIYTDSFRQLNTEVLQKSDDLFA
                     LKGENPEEEARLCLALLMGYNATIYDYGDKESKKQVILDRSLLVLESLPSSLLKCQLLT
                     YCYGEVFEEELAKEAHAIIDGWKDRELLEEEKEIVENLRGLEEPPYLFSNKSC"
     gene            5001..6302
                     /gene="aepX"
                     /locus_tag="K5L50_RS00770"
     CDS             5001..6302
                     /EC_number="5.4.2.9"
                     /GO_function="GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="aepX"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817134.1"
                     /locus_tag="K5L50_RS00770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="WP_220577311.1"
                     /sec_met_domain="CTP_transf_like (E-value: 1.6e-14,
                     bitscore: 50.2, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 9.3e-117, bitscore:
                     385.0, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 3.4e-62, bitscore:
                     205.9, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKVYVGMSADIIHPGHLNIIHEAQKLGNVTVGVLTDAAIASYK
                     RLPYLTYEQRSLIVQNLKGVDEVIPQTTLDYIPNLELLKPDFVVHGDDWKEGVQKETRQ
                     RVIETLSKWGGQVIDIPYTQGISSTKLNSKLKEIGTTPEVRLKRLRRLIEAKSIVRICE
                     SHSGLTGLIIENTSVEVNGMKREFDGMWSSSLTDSTSKGKPDIEAVDLTTRLHDLNDAL
                     ECTTKPVIFDGDTGGKIEHFVFTVRTLERLGISAIIIEDKVGLKKNSLFGTDVIQTQDT
                     IEGFCDKISAGKRAQITDDFMIIARIESFIAGKGLGDAMKRALAYIEAGADGIMIHSKD
                     KSGEDIRLFCKALREVNQSIPIVVVPTTYNHITDEELSTWGINVVIYANHMLRSAYPAM
                     LNTAKSILLHGRSYEANDLCMPVKDILELIPGTK"
     gene            6310..7437
                     /gene="aepY"
                     /locus_tag="K5L50_RS00775"
     CDS             6310..7437
                     /EC_number="4.1.1.82"
                     /GO_function="GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0032923 - organic phosphonate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="aepY"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292446.1"
                     /locus_tag="K5L50_RS00775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="WP_220577310.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 9.9e-18, bitscore:
                     60.1, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3e-20, bitscore:
                     68.4, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISPELFINSLVQNELDFFTGVPDSLLKHICAYITDHIASNSHII
                     AANEGAAIGLAVGYHLATNKIPVVYMQNSGIGNSINPLLSLADKEVYNIPLLLLVGWRG
                     EPGVKDEPQHIKQGKVTIPLFDSMRIKNQILSRDKSDFLQQLNVALEYIRETNEPFVFI
                     IQKETFSDYKLQTPDNNTLLLDRETAIKIIVSSISKNDIVVSTTGMISRELFEYRDNMG
                     ESHEKDFLTVGSMGHASQIALGIALQKKARTVYCFDGDGAVLMHMGGLTTIGSIKPSNY
                     IHVLFNNGAHDSVGGQPTVGFEVDFTKIALACGYKYALVVESASSLREVLKTVNEMQGP
                     VLLEVKVKKGARKGLGRPTITPVQNKGSFMDFLKR"
     gene            7439..8560
                     /locus_tag="K5L50_RS00780"
     CDS             7439..8560
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Fe-ADH"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292445.1"
                     /locus_tag="K5L50_RS00780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphonoacetaldehyde reductase"
                     /protein_id="WP_220577309.1"
                     /sec_met_domain="Fe-ADH (E-value: 7.9e-83, bitscore: 274.0,
                     seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQRVYIGEGSLNNLDIILQKYSAKKVFLVRGKKSYELCGAQSRL
                     NSILKKVGGQIFEFYDFEENPKIEDLDRGLLQLRVCHPDLILAIGGGSVLDMAKLLRFF
                     YSYSGNKIGEVFEKIENLLPLIVIPTTAGTGSEATSFAVLYKNKVKYSVSHEDILPEIA
                     FIDPYFTYNVSQYLTACTGFDALAQAIEAYWNLNATDESDVFAVKAIKLLWPNLPLAVN
                     KPTKEVRNSMSEGAYWAGCAINITKTTAPHAFSYPFTTYYGYPHGHAVALTFPFFMEYN
                     VGSILSKHGTVFDKMIRLMKTLNIANVNECKSLLCDYIKNIGLNFKLPANFDHRIILSN
                     INVQRLSNNPVQIDESVAKTALNTLCIKDDDKD"
     gene            8566..9810
                     /locus_tag="K5L50_RS00785"
     CDS             8566..9810
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014051.5"
                     /locus_tag="K5L50_RS00785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="oligosaccharide flippase family protein"
                     /protein_id="WP_220577308.1"
                     /sec_met_domain="Polysacc_synt (E-value: 5.9e-35, bitscore:
                     116.9, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIKKYLDSYKVIFQNTFYLSIIEAIRLIMPFIALPYIITTIGAT
                     NYGIIIFSQTIVSYFSIVINWGLDVTSVREVSLSRGSLKKMSLIVSTVFTIKFILFGLC
                     FSIFMICLYFIPFFQQYRIIYIYSFLSCFSEVIIPVWFFQGIEKMKYLTYIRTTSIVLY
                     TILVFTFIKKEMDFTFLALLQSLANITAGVFSLVLLLIFCRVKFVLPTFRVMKETFCNS
                     TPFFVSRVSFLLNTNIAKTMSGLFLNMHTVAAFDLSQKILSFVSVPIQMLNQAIYPHVA
                     KNPIKSFSVKMLYVIAALSLILSVLLFFLAPYIILYFSEQLYAEALIFIRILCLYVFCF
                     GINTYLGTPLLVAYGFYKPFNFSVIYSTLFVCSLYGILYVFNLLNGECFALAMFLTELF
                     ILIYRFYYCLKFKLI"
     gene            9816..10601
                     /locus_tag="K5L50_RS00790"
     CDS             9816..10601
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016852.5"
                     /locus_tag="K5L50_RS00790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="WP_137569114.1"
                     /transl_table=11
                     /translation="MEKISNYNTDGTMLRKQQERMFEMLCAIDDICTKNEINYWLSGGT
                     LLGAVRHGGFIPWDDDLDIQLMKDDYNKLLGLLKTELPEQYQLQTYKTDKNYFARYAKV
                     RDLNSLLEDSDYTLGYKYKGIFIDIFSVEKANKFLVKISAFMQWYLLISLLPMVHKKTK
                     MKLNLFSLWNKFLGTFYSGFRLFGKLFAKKINYSYGVYYPLNCDYTTIFPLTTIEFEGH
                     MFKAPGNYNLYLCNIYGDYMKLPPKEERENHNIKVVHIV"
     gene            10616..11941
                     /locus_tag="K5L50_RS00795"
     CDS             10616..11941
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K5L50_RS00795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_220653563.1"
                     /transl_table=11
                     /translation="MLIVILLTLILIYILYKIYKKNKIHQFLLIYFAFSAFLWVIFPSI
                     VSLIGDRYKQELVVKENVFITYAIYEFLFYIILLLLLYRFPLCKPFYYGKYIPNTSSSI
                     KRIWFVLSLFLIINIIKQISSNVTYSEVNDSTILAEQETNLFSIFSNFSICLYLAVILF
                     YNKLYSKRIIFFSWLLLSLNIVTVLAHGARMQVIFYLFVFVYYLFSSALNKKRFFLYMS
                     FLIGIIYLCSYILPAVAYIRSGGNFTVEDLRKSYENYGEANAFVLQLLIKSNSINTGAI
                     LVEETKEKPYLNPYINSIYSFCPSILFPGKRPLAGSSDGTIDGFPTRLAAEYISGDSRF
                     FNVGISTSATALWHGGIFFYVINLVIVFLWYRFLNSLMFYENYYAKLLVVYFTVLGYFI
                     LPTADVLIRDLLKQPFFYAFFIFISRGGLIKDWMRSLKRHIA"
     gene            11970..12941
                     /locus_tag="K5L50_RS00800"
     CDS             11970..12941
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012745.5"
                     /locus_tag="K5L50_RS00800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase"
                     /protein_id="WP_220577306.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.5e-35,
                     bitscore: 117.7, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNSILLSIVIPIYNVELYVERCLLSCLNQNISISRYEIILVDDG
                     SPDNSIDKISKWLDRENIILLTQSNSGLSVARNNGLLYARGKYVWFVDSDDWILENCLR
                     EITSYMDAEVDIITFCAADFIDNKSYRRFEYTIDANKKIEGKNLLRREVCQHCVPFSIY
                     RRAFLKQNDLFFLPNIFHEDTEFSPRAYYFANSVFCINKVFYYVYQNPNSITRTFNPKK
                     SFDLLVVSKSLSSFVLNVTPEFRCCFNNLISLSINAAIDGIVGSKSDMSRFMIELTSRK
                     LYRSMLNAGKLKYKIEGLLLYLCPSFLFFLYKRYSLLRRIVK"
ORIGIN
        1 cctcctaatc tggttcagtc ctttcatgaa ttagagcggg taaaccgtac cgattggttt
       61 tgtacttccg atccggtagg caaaaaactt ggttccggag gtggaacagc ttggttactg
      121 aaagaatgtt ataacgaatt tgctgacggg acctcttttt ccgggtggct gggaaaagag
      181 aaaagaattc tgttgcatgc aggcggacaa agccgtcgtc tgcccggtta cgcaccatcg
      241 ggcaagatac tgacaccggt tcctgtgttc cgatgggaga ggggacaaca ccttgggcag
      301 aacttgcttt ccttacagtt gcctctgtat gaaaagatca tgtctttagc tccggaaaat
      361 ctgcatacat tgattgcgag cggtgacgta tatattcgtt cggagaagcc tttacaaacc
      421 attccgtctg cagatgtagt ttgctatgga ctgtgggttg atccttcatt ggccactcat
      481 catggtgtat ttgcttccga ccggaaacat cctgaaaaac ttgacttcat gctccaaaaa
      541 ccttctttgg atgaactgga gtcattgtca aagactcatc tttttctgat ggatattggc
      601 atctggcttt tgagtgaccg tgctgtggag gtgttgatga agcgttccta taaagagaat
      661 tctgaggaac tgagatatta cgatctttat tccgattttg gattggctct tggcgcacac
      721 ccgtgcattg aagatgaaga ggtgaatact ttgtcggttg ctattttacc tttgcccggg
      781 ggagagtttt atcattatgg taccagtaaa gaactgattt cttccactct ttctgtgcag
      841 aataaagtgt acgatcagcg tcgtatcatg catcgtaaag taaaacccaa tccggctatg
      901 tttgtccaaa atgctgtagt gcagatacct ctctgtgcag agaatgctga tttatggatt
      961 gaaaacagcc atataggggc tcagtggaag atagcttcac gacatatcat aaccggtgta
     1021 ccggaaaatg actggacatt gactgttcct gccggagtgt gtgtagatgt ggttccgatg
     1081 ggagataagg gctttgttgc ccgtccatac ggtctggacg atgttttcaa aggagatttg
     1141 agagattcta aaacaaccct gacgggtatt ccttttggtg aatggatggc gaaacgtggt
     1201 ctttcatata cagatttgaa aggacgtacg gacgacttgc aggctgcttc tatatttcct
     1261 ttggttaact ctgctgaaga attgggattg gtgttgaggt ggatgctgtc cgaacctgag
     1321 ttggaagagg gaaagagcat ttggttacgt tccgaacgtt tctctgcaga tgagatttcg
     1381 gcaggtgcca atctgaaacg tttgtatgca cagcgtgaag agtttcggaa aggaaactgg
     1441 aaagcattgg ccgttaatca tgaaaaaagt gttttctatc agctcgatct ggccgatgcg
     1501 gccgaagatt ttgtacgtct tggtttggat atgcctgagt tattgcctga ggatgctctg
     1561 cagatgtcac gaatccataa ccggatgttg cgtgcgcgta ttttgaaatt agacgggaaa
     1621 gattatcgtc cggaagaaca ggctgctttt gatctgctgc gtgatggact gttgggcgag
     1681 atcagcaacc gcaagagtga accaaaactg gatgtatatt ccgatcagat tgtttgggga
     1741 cgcagtcctg tacgtatcga tatggcagga gggtggaccg acactcctcc ttattcactt
     1801 tattcgggag gaaacgttgt gaatctggcc attgagttga acggacaacc tcccttacag
     1861 gtttatgtga agccgtgcaa agacttccat atcgtcctgc gttctatcga tatgggtgcc
     1921 atggaaatag tatctacgtt tgatgaattg caggattata agaagatcgg ttcaccattc
     1981 tctataccga aagccgctct atcgttggca ggctttgcac ctgcgttttc tgctgtatct
     2041 tatgcttcat tggaggaaca gcttaaagat ttcggtgcag gtattgaagt gactttattg
     2101 gcggctattc cggcaggttc cggcttgggt acaagttcta ttttagcttc taccgtactt
     2161 ggtgccatta acgatttctg tggtttagtc tgggataaga atgagatttg tcaacgcacc
     2221 cttgttcttg aacaattgtt gactaccggt ggtggatggc aggatcagta tggaggtgtg
     2281 ttgcagggtg tgaagcttct tcagaccgag gcgggctttg ctcaaagccc attggtgcgt
     2341 tggctgcccg atcatttatt tacgcatcct gaatataaag actgtcactt gctttattat
     2401 accggcataa ctcgtacggc aaaaggaatc ttggcagaaa tagtcagttc catgttcctc
     2461 aattcatcgt tgcatctcaa tttgctctcg gaaatgaagg cacatgcatt ggatatgaat
     2521 gaagctatac agcgtggaag ttttgttgag tttggccgtt tggtaggaaa aacctgggaa
     2581 caaaacaaag cattggatag cggaacgaat cctccggctg tggaggcaat tatcgatctg
     2641 ataaaagatt atactttggg atataaattg ccgggagccg gtggtggcgg gtacctatat
     2701 atggtagcga aagatccgca agctgctgtt cgtattcgta agatactgac agaaaacgct
     2761 ccgaatcctc gtgcacgctt cgttgaaatg acgctgtcgg ataagggatt ccaagtatca
     2821 cgatcataaa atcattatta gtcgacggga ggtaaatact ttatctcttg tcgacttcct
     2881 tttctattcc tcattaagtt tgcctcttct gttattctga tagtaaaata gagattctgg
     2941 tatctttaag tctgcttttt tcttgtttta aaaactccgt tgtcttgaga aagtgatgaa
     3001 atagcaagtg atggagcata aacttgcctt taattcgttt cttcatacgt gtactcttca
     3061 tacgatgtta acttcgtctt tgcaaaacat taacttcttg tgaagaagaa gttagttata
     3121 agaaaatacg aagttaactt cgtatagtta cgaaattcat ttttattgaa aattctccta
     3181 ctttttgcct aacggtttca tttattgaac aaaaactata gaattctctt ctgtagcata
     3241 ggtcaatatg aatcattcct gaatgttatt ttggaggtat aggatgccgt tgccgttgct
     3301 tactgattga aattagcaca tgtttattga acttgtgact ggtgatctga tttatttggt
     3361 ggagatatga tattggtgaa aatggaatcc cggtgttttt tataacaact aagtcagtgg
     3421 tccgtaaata agggattcct gaagttactc aaaatacaat gtgacagaat gcttttggga
     3481 gtatataatt tctaaacctc tatagatgga ataatgaaga ttttattttg ttaggtaatt
     3541 tgtttattat cagtatatta tgtggtaagc gtaaaaagaa caaaaagaat aaattctttt
     3601 gtaattaact gatattcagt agtgaatgtg tttcaaaaga cgttcgtctt ttgaaacatc
     3661 aattatggag ataaaagacc acatgcaaaa taagtaaaaa gctgttttta ttatcagatt
     3721 attatctgat aatcagcata taaagaagta tattcacttt ttcatctaaa aatattttgc
     3781 acatcaaaat ttagttcata cctttgtgct attaaacgaa cgttttttga aacatcaatt
     3841 gatcgaatcc gttcgtttcc acctaaaata agtcataaaa aatcttgcag tttggctgag
     3901 aaaaagagtc tcacccaact gcgaagcata cccggactta agtaaatcac tcaggaatat
     3961 atccttctaa atgtctcaac aacaagaata ttggttcgct gcccgtaccc gtaaggatca
     4021 ggagcttact actcgtgacg cattagagaa aataggcgtc gagtactttt tgccgaccca
     4081 atttgtcatt cgtcaattga aatatcgccg gcgtcgtgta gaagttcctg tcattcgtaa
     4141 tcttattttt gtacatgcta ccaaagagtt tgcttgtgct attgccaacg aatacggggt
     4201 tcgtcttttc tatatgcgcg attttgacac taagagtatg cttattgttc ccgataagca
     4261 gatgaaagat tttatgtttg tcatgaatct cgaccctgct gctgttatcc tcaacgatga
     4321 ttgtttcgca gttggcacca aagtacaagt tatcaaggga gatttttgtg gagttgaagg
     4381 tgaactggcc agtctttcta atcgtactta tgtcactatc cgtattcgtg gcgttttatc
     4441 tgccagtgtc aaggttccta aaagctacct tcgcattctc gcaccgtaat cagtaatatt
     4501 ttatcatata aaatgaatac tcttatgacc cagattcatg agctgcaaca tgtggctcac
     4561 gaattattgt atttgggtgc tgacggttct cctatctata ccgacagttt ccgtcaatta
     4621 aacactgaag tgcttcaaaa atctgatgat ttgtttgctt tgaaaggaga aaatccggaa
     4681 gaagaagcac gactttgcct ggctctcttg atgggctaca atgcaacaat ctatgattat
     4741 ggcgacaagg aatctaagaa acaggttatt ctggatcgtt ccttgcttgt gttagagtct
     4801 ctcccgtctt ctttgctcaa gtgtcagctt ttgacatatt gttatggaga agtttttgag
     4861 gaagagttgg caaaggaagc tcatgcaatt atagatggtt ggaaagatcg agaattgtta
     4921 gaagaagaaa aagaaatagt tgaaaacttg aggggtttgg aagaacctcc atatttgttt
     4981 agtaataaat cttgttagaa atgaaaaaga aagtatatgt tgggatgagt gctgatatta
     5041 ttcatccagg gcatttgaat attattcatg aagcacaaaa actaggaaac gtgactgtag
     5101 gtgttttaac agatgctgct atagccagtt ataaacgttt accctattta acttatgaac
     5161 aacgttcttt aatagtacaa aatctaaaag gtgttgatga agtaattcca caaactacct
     5221 tagattatat tcctaatctt gaattgctaa aacctgattt tgttgttcat ggagatgatt
     5281 ggaaagaagg cgttcagaag gaaacaagac aacgggttat tgaaactctt tctaaatggg
     5341 gtggacaagt tattgatata ccttatacac aaggtatatc ctcaacaaaa ctcaattcaa
     5401 agcttaaaga aatagggaca actcctgaag tgcgtttgaa aagattaaga cgtttgattg
     5461 aagctaagtc cattgttcgt atttgtgaat cacatagtgg attaacgggg ttaatcatag
     5521 aaaatacgtc tgttgaggta aatggtatga agcgtgaatt tgatggaatg tggtccagta
     5581 gtttaactga ttcaacatca aaggggaaac ctgatattga agccgtagat ttgacgaccc
     5641 gtttacatga tttaaatgat gctttggaat gtacaacgaa acctgttatt tttgatggtg
     5701 atactggagg aaaaattgaa cattttgttt ttacagttcg tacgttagaa cgtttgggca
     5761 tttctgctat aataattgaa gataaagttg gattgaaaaa gaattcttta tttggaactg
     5821 atgttattca gacacaagat acaattgagg gcttttgcga taaaatatca gcaggaaaac
     5881 gtgctcaaat aacggatgac tttatgatta ttgccagaat tgaaagtttt attgctggta
     5941 aaggcttagg tgatgctatg aaaagagcat tagcttatat tgaggctgga gctgatggta
     6001 taatgattca cagtaaagat aaatcaggtg aagatattag acttttttgt aaggcgttac
     6061 gagaggttaa tcaatcaatt cctatagtag tagttccaac aacttataat catataacag
     6121 atgaagagtt gtctacatgg ggaataaatg tggtcattta tgcaaaccat atgttacgta
     6181 gtgcttatcc tgctatgctg aatactgcga aatctatatt attacatggt cgttcatatg
     6241 aggcaaatga tttatgtatg ccagtaaaag atatattaga attaatacca ggtacaaaat
     6301 agttttttta tgatttctcc agaacttttc attaactctc ttgtacaaaa tgaacttgat
     6361 ttttttacag gagtaccaga ctcattattg aaacatattt gtgcctatat aactgatcat
     6421 atagcttcaa atagtcatat aattgctgcc aatgaggggg cagcgattgg tttggctgtt
     6481 ggttatcatt tggcaactaa taaaattcca gttgtatata tgcaaaattc cggtattggt
     6541 aattctatta atcctttatt atctttagca gacaaggaag tgtataatat tcctttatta
     6601 ttattagtcg gttggagagg tgagcctggt gtaaaggatg aacctcaaca tataaaacaa
     6661 ggtaaggtaa ctattccttt gtttgattct atgcgaataa agaatcagat actttccaga
     6721 gataaatctg attttttaca acaactaaat gtagcattag aatatattag agagacaaat
     6781 gagccttttg tttttataat tcagaaagaa acattcagtg attataaact gcaaactcca
     6841 gataataata ctttgttatt ggatagagaa actgctatta agattattgt ttcttctatc
     6901 tctaaaaatg atatagtcgt ttctactaca ggaatgatat ctcgtgagct atttgaatat
     6961 agggataaca tgggtgaatc ccatgaaaag gattttttaa cagttggttc tatggggcat
     7021 gcctctcaaa tagctttagg tattgcatta caaaaaaagg ccagaactgt atattgtttt
     7081 gatggtgatg gtgctgtttt gatgcatatg ggaggactaa caaccattgg atcaataaaa
     7141 ccgtctaatt acatccatgt attatttaat aatggagcgc atgattctgt aggtgggcaa
     7201 cctacggttg gttttgaggt agactttaca aagatagctt tggcgtgcgg ttataaatat
     7261 gctttagttg tggagagtgc ttcatcttta agagaagttc ttaaaacggt gaatgaaatg
     7321 caaggacctg ttctcttgga ggttaaggta aagaaaggtg ctcgaaaagg tttaggtcgt
     7381 cctacaataa ctccagtaca aaataaaggt tctttcatgg actttttaaa gcgataatat
     7441 gaaacaacgc gtatatattg gagaaggctc gttaaataat ttagatatta ttttgcagaa
     7501 atattctgca aagaaggttt ttttggtgag agggaagaaa tcatatgagc tttgtggagc
     7561 acaatcaaga ttaaactcta ttttgaagaa agtagggggg cagatttttg aattttatga
     7621 ttttgaggaa aatcctaaaa tagaagattt agatagagga cttttgcaat taagagtttg
     7681 tcatccagat ttgatacttg caataggagg aggcagtgta ttggatatgg ctaagttgct
     7741 tcgttttttt tattcttatt ctggaaataa aataggagaa gtttttgaaa aaatagaaaa
     7801 tctattacct ttaattgtta tacctacaac tgcgggaact ggtagtgagg ctacgagttt
     7861 tgcagtgtta tataagaata aagttaagta ttctgtttcg catgaagata tattacctga
     7921 aatagctttt attgatccat attttacata taatgtttct caatatttga cggcatgcac
     7981 gggctttgat gcattggccc aggctattga agcttattgg aatttaaatg ctacagatga
     8041 atctgatgtt tttgctgtaa aagcaataaa attgctttgg cctaatttac cattagcggt
     8101 aaataaacca acaaaagagg ttagaaacag tatgtctgaa ggggcctatt gggctggatg
     8161 tgcaataaat ataacgaaga ctacagcacc tcatgctttt tcttatccat ttactactta
     8221 ttatggctat ccacatggac atgcggtggc attaactttc ccatttttta tggaatataa
     8281 tgtaggaagt atattatcaa agcatggtac agttttcgat aaaatgattc gattgatgaa
     8341 gacactcaat atagccaatg taaatgaatg taaatcttta ctgtgtgatt atataaaaaa
     8401 tatcggactt aattttaagt tgcctgcaaa ttttgatcat cgaattattc tttcaaatat
     8461 aaatgtgcaa aggttaagta ataaccctgt acagatagat gagtctgtgg ctaaaacagc
     8521 tttaaatact ttatgtatca aagatgatga taaagattga tagtgatgaa aattaaaaag
     8581 tatttagatt catataaagt aatttttcaa aatacatttt atttgtcgat aattgaagct
     8641 atacgtttaa taatgccctt tatcgctctt ccatatatta ttactactat tggtgcaact
     8701 aattatggga ttattatttt ttctcagacg attgtttcat atttttcaat tgtcattaat
     8761 tggggattag atgtaacttc agtccgagaa gtgtccttaa gtaggggcag tttaaaaaaa
     8821 atgagtttaa tagtttctac tgtatttact attaaattta ttttgtttgg actttgcttt
     8881 tctattttca tgatatgttt gtattttatc cctttcttcc aacaatatag aattatatat
     8941 atctattctt ttttgtcatg tttctctgag gttattatac ctgtgtggtt ttttcaaggt
     9001 attgagaaga tgaagtattt gacttatata agaacaacaa gtatagttct ctatactata
     9061 cttgttttta cttttattaa aaaagaaatg gactttacat ttttagcctt gttacaatct
     9121 ctggctaata ttactgcagg tgtattttct ttggtgttat tattaatttt ttgtagagtc
     9181 aaatttgtac ttcctacttt cagagtgatg aaagaaactt tttgtaatag tacaccgttt
     9241 ttcgtttcca gagtttcgtt tttattgaat acgaacatag ctaaaacgat gagtgggtta
     9301 tttctaaata tgcacactgt tgccgcattt gatttgtctc agaaaatttt atcttttgtg
     9361 tctgttccca tacaaatgct gaatcaggcg atttacccac atgttgcaaa aaatccaatt
     9421 aaatcatttt ctgtaaagat gctatatgtt atagctgcat tatctctgat tttatctgtt
     9481 ttactttttt ttctagcacc ctatatcatt ctttattttt cagagcaact ctatgctgaa
     9541 gctctaatct ttataaggat tttgtgcctt tatgtatttt gttttggtat taatacttat
     9601 ttagggaccc cacttcttgt cgcttatggt ttttataagc catttaactt tagtgtaata
     9661 tattctactc tatttgtgtg ttctttgtac gggattttat acgtgtttaa tttattgaat
     9721 ggggagtgtt ttgctttagc aatgttctta acagaacttt ttattctgat atatagattt
     9781 tattattgtt tgaaatttaa attgatataa aaatagtgga aaagatatct aactataata
     9841 ctgatggtac tatgttacga aagcaacaag aacgtatgtt cgaaatgtta tgtgcaatag
     9901 atgatatttg taccaagaat gaaattaatt attggttatc tggtggaact cttttaggag
     9961 ctgttaggca tggaggtttt ataccatggg atgatgattt agatattcaa ttgatgaaag
    10021 atgactataa taaattatta ggtttattaa agacagaact tcctgaacaa taccagttac
    10081 aaacatataa gactgataaa aactatttcg caagatatgc gaaagtaaga gatttgaatt
    10141 ctttgcttga agatagtgat tatactttgg gatataaata taaagggata tttatagata
    10201 ttttttctgt tgaaaaagca aataagtttt tagtaaagat atctgctttt atgcaatggt
    10261 atctattgat ctctttgtta cctatggtac ataagaaaac taaaatgaaa ttgaatcttt
    10321 tttcattatg gaataagttt ttaggtactt tttattctgg attcagacta tttggtaaat
    10381 tgtttgccaa gaagattaat tattcttatg gagtttacta tcctttaaat tgtgattata
    10441 ctactatatt tccattaaca acaattgagt ttgagggaca tatgtttaaa gccccgggga
    10501 attataattt atatttatgt aatatatatg gtgactacat gaaacttcct ccaaaggaag
    10561 agagagaaaa tcataatata aaagtggttc atattgttta gttcgcacta tatttatgtt
    10621 aatagttata ctactgactc taatcttaat atatatatta tataagatat ataaaaaaaa
    10681 taaaattcat caatttttac ttatatattt tgctttttct gcttttttgt gggttatttt
    10741 tccatcgatt gtttctttaa ttggagatag atataaacag gaattggtag taaaagaaaa
    10801 tgtttttata acttatgcta tttatgaatt tcttttttat ataatattgt tacttttgct
    10861 ctatcgtttc ccattatgta agcctttcta ttacgggaaa tacattccca atacatcctc
    10921 gtctataaaa cgaatatggt ttgtattaag tctttttctt attattaata ttatcaagca
    10981 gatatcttcc aatgttactt attctgaagt caatgattca actatattgg ctgaacagga
    11041 aactaattta ttttcaattt tttcaaattt ctctatatgc ctatatcttg ctgtaatatt
    11101 attttacaat aaactttatt caaaaagaat catttttttc tcttggctat tactatctct
    11161 taatatagta acagttttag ctcatggagc aagaatgcaa gttatttttt atttgtttgt
    11221 ttttgtatat tatttgtttt cttctgcatt aaataagaaa cgttttttcc tttatatgtc
    11281 tttcctgatc ggaattattt atttatgttc ttatattctt ccggcagttg catatatcag
    11341 gtcaggagga aattttacag ttgaggattt gagaaagtca tatgagaact atggagaagc
    11401 aaatgctttt gttcttcaat tgctgattaa gtctaatagt ataaatactg gggccatttt
    11461 agttgaggaa acaaaagaaa aaccatactt gaacccatat ataaattcaa tatattcgtt
    11521 ctgtccgagt atactctttc ctggcaaaag acccttggca ggtagtagtg atggtacgat
    11581 tgacggattt ccgacaagat tagctgcaga atatataagt ggtgattctc gtttctttaa
    11641 tgtaggaata tcaacttctg ctacagcact ttggcatggt ggaatttttt tttatgtgat
    11701 taatttagtt atagttttcc tttggtatcg tttcttgaat tcattaatgt tttacgaaaa
    11761 ctattatgca aaacttttag ttgtttattt tacggtttta gggtatttta tacttcctac
    11821 ggcagatgtt ttaattcgcg atttacttaa gcagcctttt ttttatgcat tctttatatt
    11881 tatttcaaga ggggggctta ttaaagactg gatgagatct ttgaaacgtc atatcgctta
    11941 gtaaagagat cttgttttaa tatgttgtca tgaataattc tatacttcta tctattgtaa
    12001 ttcctatata taatgttgaa ttatatgttg aacgctgttt attaagttgt ttaaaccaga
    12061 atatctcaat atcacggtat gaaataattt tagtcgatga tggaagtcca gataacagta
    12121 ttgataagat ttcaaaatgg ttagatagag aaaatataat attattgacg cagtctaata
    12181 gtggattaag tgttgcacga aataatggat tactgtatgc aagaggtaaa tatgtttggt
    12241 ttgttgattc tgatgattgg attctggaaa attgcctgcg agaaataact agctatatgg
    12301 atgccgaagt ggatattatt actttttgtg cagcagattt tattgacaat aaatcttatc
    12361 ggagatttga gtatacaata gatgcaaata agaaaattga aggaaagaac ctactgagaa
    12421 gggaggtttg ccaacattgt gtgccatttt ctatatatcg aagagcgttt ttaaagcaaa
    12481 atgatttgtt ttttctgccg aatatttttc atgaagatac agaattttct cctcgtgctt
    12541 attattttgc taatagtgtg ttttgtatca ataaagtatt ttattatgtg tatcaaaatc
    12601 ctaattcaat aacaagaaca tttaatccga agaaatcttt tgacttatta gttgtatcta
    12661 aaagtttgtc ttcattcgta ttgaatgtta ctcctgaatt tagatgttgt tttaataatt
    12721 tgatttcttt gtctataaat gctgcaatag atggcattgt tggatcaaaa agtgatatgt
    12781 caaggtttat gatagagtta acttctagaa aactgtatcg gagtatgctt aatgccggaa
    12841 aattaaaata taaaatagaa ggattgctgc tttatttgtg tccttcattt ttgttttttt
    12901 tgtataaacg ttactcttta ttgaggagaa ttgttaaata atctaataat agtgttatgg
    12961 tgtttgttaa gccaattaag attgccgtaa taggtcttgg ttatgttggg cttcccttgg
    13021 cccgattgtt ttcgacaaaa tatgaatgta ttggttttga tctgaaccag tctcgtgttg
    13081 atgctttaaa ttccggtcat gatatgactt tagaggtttc tgacgactta cttcggaaag
    13141 cgatagaaaa tggtttcaaa tgtaccagta atattgacga aataaggaat tgtaactttt
    13201 atgttgtagc tgtaccaacc cctgtggatt tgaataatcg ccccgatctt acacccttga
    13261 ttggtgcgag tactacagtt ggaaaagtta tttcaaaagg ggatattgtt gtgtatgaat
    13321 ctactgtata tccgggagta actgaggaag aatgtcttcc ggtagtcgaa aaagtgtcag
    13381 ggttaacata caatgtagat ttttatgcag gttattcacc ggaacgtata aacccgggtg
    13441 ataaagaaca tactgttgaa aagattaaga aagtaacttc cggttcgact cctgagattg
    13501 ctgacattgt tgattctata tataattcag tgttggttaa cggtactcat aaagcccctt
//
