LOCUS       NZ_CP080647            10405 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces akebiae strain MG28 chromosome, complete genome.
ACCESSION   NZ_CP080647
VERSION     NZ_CP080647.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces akebiae
  ORGANISM  Streptomyces akebiae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10257202)
  AUTHORS   Ping,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v. 3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019599145.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 09:26:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,127
            CDSs (total)                      :: 9,036
            Genes (coding)                    :: 8,727
            CDSs (with protein)               :: 8,727
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 142 of 309
            Pseudo Genes (incomplete)         :: 231 of 309
            Pseudo Genes (internal stop)      :: 20 of 309
            Pseudo Genes (multiple problems)  :: 77 of 309
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:57:32
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8013773
            Orig. end                         :: 8024178
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080647.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Mo Ping.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="23"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            270..986
                     /locus_tag="K1J60_RS34680"
                     /old_locus_tag="K1J60_34680"
     CDS             270..986
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020114333.1"
                     /locus_tag="K1J60_RS34680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_34680"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_220649660.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDGVEVVHRSFELDP
                     GRAKDDVQPVLTMLTKKYGMSMAQAQAGEHNLREQAAAEGLAYRAEGRDHGSTFDMHRL
                     IHFAKDQGRQSELLQAFYRANFAEERSVYVDADEYLVELAVEAGLDEQAARKVLADPDA
                     YADAVRADEREAAELGANGVPFFVLDRKYGVSGAQPAEVFEKALAQAWGERPAPLRVVA
                     AAGAEACGPDGCAVPR"
     gene            1093..2142
                     /locus_tag="K1J60_RS34685"
                     /old_locus_tag="K1J60_34685"
     CDS             1093..2142
                     /NRPS_PKS="Domain: Aminotran_5 (16-343). E-value: 2.3e-26.
                     Score: 84.5. Matches aSDomain:
                     nrpspksdomains_K1J60_RS34685_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477739.1"
                     /locus_tag="K1J60_RS34685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_34685"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_220649661.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.2e-19, bitscore:
                     69.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFVPKNTYLNTASTGLLPARAVDAMKAAVESVAAG
                     RPQDMFADVEAARAAFGRITGVPDRRVAAGASVAVYSGLIAAALPAGAEVLTAEGEFAS
                     LVNPFHVRGDLKVRQVPLERVAESVRPGTALVAVSAAQSADGRLADLDAIREAARERGA
                     RTFVDASQSAGWLPIDADTYDYVVAVAFKWLVCPRGVTFLVVPEDLGGLTPVFAGWVAG
                     EEPWDSCYGPVEELARSARRFDESPSLFSYAGARHSLALIEELGVANVREHDLALADRF
                     RAGLARLGHEPVPAPGSAIVSVPGLGRRQAELSRAGVEVSDRAGNLRAAFHLYNTRTDV
                     DRLLDVLSG"
     aSDomain        1141..2121
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K1J60_RS34685_Aminotran_5.1"
                     /evalue="2.30E-26"
                     /label="K1J60_RS34685_Aminotran_5.1"
                     /locus_tag="K1J60_RS34685"
                     /protein_end="343"
                     /protein_start="16"
                     /score="84.5"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPARAVDAMKAAVESVAAGRPQDMFADVEAARAAF
                     GRITGVPDRRVAAGASVAVYSGLIAAALPAGAEVLTAEGEFASLVNPFHVRGDLKVRQV
                     PLERVAESVRPGTALVAVSAAQSADGRLADLDAIREAARERGARTFVDASQSAGWLPID
                     ADTYDYVVAVAFKWLVCPRGVTFLVVPEDLGGLTPVFAGWVAGEEPWDSCYGPVEELAR
                     SARRFDESPSLFSYAGARHSLALIEELGVANVREHDLALADRFRAGLARLGHEPVPAPG
                     SAIVSVPGLGRRQAELSRAGVEVSDRAGNLRAAFHLYNTRTDVDRL"
     gene            2197..2805
                     /locus_tag="K1J60_RS34690"
                     /old_locus_tag="K1J60_34690"
     CDS             2197..2805
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477737.1"
                     /locus_tag="K1J60_RS34690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_34690"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_220649662.1"
                     /transl_table=11
                     /translation="MVVPVEPTLEQPSIDVELHRRLVYGDESALREAYAAYGGLVRRVA
                     VRVTRSPAAAEDVAQEVFAQLWSRPYGFDARRGSLRTWLSMVAHRRAVDWVRGEARHRK
                     DVRADDSALHAIPDPAPDPAEAVVDRERSLLLHSALAELPRPQREVVHLAYFAGRTYRQ
                     AAVELGIPEGTAKSRLRSALRTLAESLADPPDPAAARGA"
     gene            2882..3961
                     /locus_tag="K1J60_RS34695"
                     /old_locus_tag="K1J60_34695"
     CDS             2882..3961
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477735.1"
                     /locus_tag="K1J60_RS34695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_34695"
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_220649663.1"
                     /transl_table=11
                     /translation="MTNDHDGVRELLAAWAVGALPPGDRLGVVQHLAACTSCAAEAERL
                     RDTVRLLDGTPEPSPTPWAEPGTTAPPDGAVHGRPDGAVHASADGVLSLALRSRRPRAA
                     EIAHHAAPYAAAVAGLQALVPEVEGHWGTPVVHDWDAHATVAHLVAADEHLAVHLGVDR
                     RLPVSPIPEGTPAQDAWARRTADVIAHEHGRTPAQTVATWAAQAAALLATPEAADPELA
                     ARPVTVMGLRLPVADHFVIRAFEAWIHTDDIGRALGLPVPPPPDEHLWSLVRLAVRILG
                     PALHGAPPVLFEVTGPTGTSWILGDDTDPVHAELSLDPVDFCLLVGGRHAPEEVPSTIT
                     GDEDAARNVLERAASLAWL"
     gene            complement(4079..4993)
                     /gene="thpD"
                     /locus_tag="K1J60_RS34700"
                     /old_locus_tag="K1J60_34700"
     CDS             complement(4079..4993)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="K1J60_RS34700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_34700"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_192829731.1"
                     /sec_met_domain="PhyH (E-value: 2.4e-40, bitscore: 138.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTTMNTATTVPDLYPSRGATEVSVPRQDPVVWGSPGTPGPVS
                     LAELQAYERDGFLPVEQLIAPDEVAVYRQELERLVADPAIRADERSIVEPKSQEIRSVF
                     EVHRISELFARLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHA
                     EDGLPNMRTVSVSIALTENYDTNGGLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGT
                     PSDEALTAMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTA
                     VEPFAAPVRRPEYIGARDFTPVR"
     gene            complement(5001..5405)
                     /locus_tag="K1J60_RS34705"
                     /old_locus_tag="K1J60_34705"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="K1J60_RS34705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_34705"
                     /product="ectoine synthase"
                     /protein_id="WP_220649664.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.8e-54, bitscore:
                     180.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKSASGTWESKRIVLARERVGFSLHETTLY
                     AGTETSMWYANHVEAVVCVEGEAELTDQETGRSYTITPGTMYLLDGHERHTLRVKEDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            complement(5470..6741)
                     /gene="ectB"
                     /locus_tag="K1J60_RS34710"
                     /old_locus_tag="K1J60_34710"
     CDS             complement(5470..6741)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 2.7e-72.
                     Score: 235.6. Matches aSDomain:
                     nrpspksdomains_K1J60_RS34710_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="K1J60_RS34710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_34710"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_220649665.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5e-76, bitscore:
                     255.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMFDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRAFLESFQNLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALADLCARQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTATATLETYWADGSVMEKQTRKRGEQVEQAF
                     ISITEENLADVKEYRGRGLVWGMEFHDKERAGRVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGLRVLARAVRETV"
     aSDomain        complement(5644..6657)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K1J60_RS34710_Aminotran_3.1"
                     /evalue="2.70E-72"
                     /label="K1J60_RS34710_Aminotran_3.1"
                     /locus_tag="K1J60_RS34710"
                     /protein_end="366"
                     /protein_start="28"
                     /score="235.6"
                     /tool="antismash"
                     /translation="FDRAQGSRMFDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRAFLESFQNLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALADLCARQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TATATLETYWADGSVMEKQTRKRGEQVEQAFISITEENLADVKEYRGRGLVWGMEFH"
     gene            complement(6878..7408)
                     /gene="ectA"
                     /locus_tag="K1J60_RS34715"
                     /old_locus_tag="K1J60_34715"
     CDS             complement(6878..7408)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004616.1"
                     /locus_tag="K1J60_RS34715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_34715"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_192829732.1"
                     /transl_table=11
                     /translation="MTAAQAEPLTEIAEGIRIDRPDVSDGASLWRIAGDSGALDLNSSY
                     SYLLWCRDFADTSAVARDESGEPVGFVTGYVRPERPDTLLVWQVAVDEAHRGLGLAGAL
                     LDGLTARVARRHPLTTVETTITPGNTASERLFAAYAERHGASVERTVLFEAGDFPDGPH
                     QPEVLHRIGPLSP"
     misc_feature    complement(7564..7579)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7574..7589
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     gene            complement(7751..8851)
                     /locus_tag="K1J60_RS34720"
                     /old_locus_tag="K1J60_34720"
     CDS             complement(7751..8851)
                     /NRPS_PKS="Domain: Aminotran_5 (16-305). E-value: 1.3e-28.
                     Score: 91.9. Matches aSDomain:
                     nrpspksdomains_K1J60_RS34720_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477727.1"
                     /locus_tag="K1J60_RS34720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_34720"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_220649666.1"
                     /sec_met_domain="Aminotran_5 (E-value: 9.3e-22, bitscore:
                     76.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSASHFASIENRVARLLSTEQDVVITQGEALLPLE
                     GAIRGAAGPGTTALNVITGPYGQTFGNWLRDCGATVVDLAVPFHTAVTAEQIRDAFAEH
                     PSIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAISVSERAWARMAANPRAPRRSYLSLLDWKERWIDGGRRALLHAP
                     AQLEMLALEACLERIEAEGLDTVMSRHASAAAATRAGALALGGGLEPYVREAADAAPVA
                     TTLRAPAGVDASELVAKTLATDPELPLAAGGGALAAEMIRVNHYGPDATSDVVHRALAG
                     LGAALAEFGAAVDPEGARRAAAEAWA"
     aSDomain        complement(7937..8803)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K1J60_RS34720_Aminotran_5.1"
                     /evalue="1.30E-28"
                     /label="K1J60_RS34720_Aminotran_5.1"
                     /locus_tag="K1J60_RS34720"
                     /protein_end="305"
                     /protein_start="16"
                     /score="91.9"
                     /tool="antismash"
                     /translation="ASIENRVARLLSTEQDVVITQGEALLPLEGAIRGAAGPGTTALNV
                     ITGPYGQTFGNWLRDCGATVVDLAVPFHTAVTAEQIRDAFAEHPSIDFVSLVHAEAATG
                     NTNPVAEIGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSAI
                     SVSERAWARMAANPRAPRRSYLSLLDWKERWIDGGRRALLHAPAQLEMLALEACLERIE
                     AEGLDTVMSRHASAAAATRAGALALGGGLEPYVREAADAAPVATTLRAPAGVDASELVA
                     KTLATDPE"
     gene            9025..9885
                     /locus_tag="K1J60_RS34725"
                     /old_locus_tag="K1J60_34725"
     CDS             9025..9885
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338057.1"
                     /locus_tag="K1J60_RS34725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_34725"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_220649667.1"
                     /transl_table=11
                     /translation="MNSVPGRRTRILAATTAAAGLLLVAGCSSDDGGSGGTKTAAGGVE
                     LVKAGQLTTCTHLPYPPFQSEIDGEVQGFDVSLIDLVADDLGVKQEILDTPFENFKTGA
                     FLNSGECDLAAAGMTITEERKKNVDFSDPYFEATQAVLAAKKADIGSFADLKGDGDYKV
                     GTQAQTTGEDYAKSQGLDPVSFESSDAVLNGLRTGQVDAVVIDYPVVQGWLKSADLADA
                     YTVAEQINTGEEYGFTVKKGNTKLLDAVNKALADAKSDGTYKKLYEQWIGPYDESAASA
                     SPSAS"
     misc_feature    10246..10260
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     misc_feature    complement(10360..10374)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.71"
                     /tool="antismash"
ORIGIN
        1 cccgcgacgg caagcggtgg accttcgtcc gtctcttcac cctggccggc gagcgggaga
       61 gcggcgccgc cctggtcggc ttcatgacgc agtccccgat gggcgagggc tgcgtcgtca
      121 cctacgacga gatcgagttc cgcccgaact ggccggagga cctgcgcaac ggcagctgac
      181 cggggaatga gccgcgctgc ttgaacgttc atccaccgta gaagcggagg ccgtctccgt
      241 acccgtacga cctggagaga gctgacacca tgcgcgtcga gatctggagc gacatcgcct
      301 gcccctggtg ctacgtgggc aaggcccgct tcgagaaggc actcgcggcc ttcccgcacc
      361 gcgacggtgt cgaggtggta caccgctcct tcgagctcga cccgggacgc gccaaggacg
      421 acgtgcagcc cgtgctcacc atgctgacca agaagtacgg gatgagcatg gcgcaggccc
      481 aggccggtga gcacaacctc cgggagcagg cggcggccga ggggctcgcc taccgggccg
      541 agggccgtga ccacggcagc accttcgaca tgcaccgcct gatccacttc gccaaggacc
      601 agggacggca gagtgaactg ctccaggcct tctaccgggc gaacttcgcg gaggagcggt
      661 ccgtgtacgt cgacgcggac gagtacctgg tcgagctggc cgtcgaggcg gggctcgacg
      721 agcaggccgc gcgcaaggta ctggccgacc ccgacgcgta cgcggacgcc gtccgcgcgg
      781 acgagcgcga ggcggcggag ctgggggcga acggggtgcc gttcttcgtg ctcgaccgga
      841 agtacggggt ctccggggcc cagcccgcgg aggtcttcga gaaggcgctc gcccaggcgt
      901 ggggggagcg gcccgcgccg ctgagggtcg tggcggcggc cggcgcggag gcctgcgggc
      961 cggacggatg cgcggtgccg cggtaacggg ggcgctgcgg cgcaggtcag acgccatgca
     1021 taagcgttgc ttggggacag ggcgcagaac atctcaatgg acgtggggtg cgcccggccc
     1081 cacagtggtc ccatggaaac cttcgagagc ctcgttcgtg ccgagttcgt cccgaagaac
     1141 acctatctga acaccgccag taccggcctg ctgcccgccc gggcggtcga cgcgatgaag
     1201 gccgccgtgg agtccgtggc ggccgggcgg ccccaggaca tgttcgccga cgtggaggcc
     1261 gcgcgggcgg cgttcggccg gatcaccggg gtgccggacc gccgcgtggc ggccggtgcc
     1321 tcggtcgccg tctacagcgg gctgatcgcc gccgcgctgc ccgcgggcgc cgaggtgctg
     1381 accgccgagg gcgaattcgc ctccctcgtg aaccccttcc atgtgcgcgg cgacctcaag
     1441 gtgcgtcagg tgccgctgga gcgcgtcgcc gagtcggtgc ggcccggcac ggcactcgtc
     1501 gcggtgagcg ccgcgcagtc cgccgacggc cgcctcgccg acctggacgc gatccgcgag
     1561 gcggcccggg agcggggagc gcgtacgttc gtcgacgcgt cacagtccgc cggctggctg
     1621 ccgatcgacg cggacacgta cgactacgtc gtcgccgtcg ccttcaaatg gctcgtctgc
     1681 ccgcgcggag tgaccttcct cgtcgtcccg gaggacctcg gcggtctcac gcccgtcttc
     1741 gccggctggg tcgcggggga ggagccgtgg gacagctgct acggcccggt cgaggaactc
     1801 gcccgctccg cccgccggtt cgacgagagc cccagtctgt tctcgtacgc cggcgcccgc
     1861 cactccctcg cgctgatcga ggagctgggc gtggcgaacg tacgggagca cgacctcgcg
     1921 ctggccgacc gcttccgggc cggcctcgcc cgcctcggcc acgagccggt gcccgcgccc
     1981 ggctcggcca tcgtgtcggt gcccggactc gggcggcggc aggcggaatt gagccgggcg
     2041 ggggtggagg tctccgaccg tgccgggaat ctgcgtgccg ccttccacct gtacaacacg
     2101 cggacggatg tcgaccggct tctggatgtc ctgtccggct gactttcccg acccactggc
     2161 gcggaccgcg ccaggacgct aggaaggcag cacgaggtgg tggtgccggt ggagccgacg
     2221 ctcgaacagc cgtccatcga tgtggagttg catcggcggc tggtgtacgg ggacgagtcc
     2281 gcgctgcgtg aggcgtacgc ggcgtacggg ggactcgtcc gacgggtggc cgtccgggtc
     2341 acccggagcc cggcggccgc cgaggacgtg gcgcaggagg tcttcgcgca gctgtggagc
     2401 aggccgtacg gcttcgacgc gcgccggggc tcactgcgca cctggctgtc catggtcgcc
     2461 caccggcggg ccgtggactg ggtgcgcggc gaggcccggc accgcaagga cgtccgcgcg
     2521 gacgactcgg cgctgcacgc catccccgac ccggcccccg acccggccga ggcggtcgtc
     2581 gaccgcgagc gctccctcct gctgcacagc gccctcgccg aactcccccg cccccagcgg
     2641 gaggtggtcc acctcgccta cttcgcgggc cgcacctacc gccaggccgc cgtcgagctg
     2701 ggcatccccg agggcaccgc gaagagccgc ctccgatcgg cgctgcgcac actggcggag
     2761 tccctcgcgg acccaccgga ccccgcagcg gcaaggggcg cgtgatgggg gcgcacggga
     2821 ccgtccggag cgcgcggcac ctgcgtatcg tggacgtggc agggcgaaag ggcggcgcgc
     2881 gatgaccaac gaccacgacg gcgtacggga gctgctggct gcctgggccg tcggtgccct
     2941 cccgcccggc gaccggctcg gagtcgtcca gcacctggcc gcctgcacgt cgtgcgcggc
     3001 ggaggcggaa cggctccgcg acacggtacg actgctggac ggcaccccgg agccgtcccc
     3061 gacgccgtgg gcggagccgg ggacgaccgc cccgccggac ggcgcggtgc acgggcgccc
     3121 ggacggcgcg gtgcacgcga gcgccgacgg cgtcctctcg ctcgccctcc gctcccgccg
     3181 cccccgcgcc gccgagatcg cccaccacgc ggcgccctac gccgcggccg tggccggact
     3241 ccaggcgctg gtaccggagg tggagggcca ctggggcacc cccgtcgtgc acgactggga
     3301 cgcgcacgcc accgtcgccc acctcgtcgc cgccgacgaa cacctcgccg tacacctggg
     3361 cgtcgacagg cggctgcccg tctcgcccat cccggagggg acgcccgccc aggacgcctg
     3421 ggcccggcgc accgccgacg tcatcgccca cgagcacggg cgcacgcccg cgcagacggt
     3481 cgccacctgg gccgcgcagg ccgccgcgct gctcgccacc cccgaggccg ccgaccccga
     3541 actcgccgcc cggcccgtca cggtgatggg cctgcggctg ccggtcgccg accacttcgt
     3601 gatccgcgcc ttcgaggcct ggatccacac ggacgacatc ggccgggccc tcggtcttcc
     3661 cgtcccgccg ccccccgacg aacacctgtg gtccctggtc cggttggccg tccgcatcct
     3721 cggcccggcc ctccacggcg cgccccccgt tctcttcgag gtcaccgggc ccaccggcac
     3781 cagctggatc ctcggcgacg acaccgatcc cgtgcacgcc gaactcagcc tggatccggt
     3841 cgacttctgc ctcctggtcg gcggccgcca cgccccggag gaggtaccga gcaccatcac
     3901 gggcgacgag gacgcggcac ggaacgtact ggagagggcg gcgtcgttgg catggctctg
     3961 agcgttcgcc cggcgcgcca ggcgcgcacc cgggcggtcg cgcacgcagg cgtgcgaaag
     4021 gggccgggcg gtcgctcagg ggtggcgacc gcccggcccc ctcagggggg cggtgggctc
     4081 accgcaccgg ggtgaagtcc cgggcgccga tgtactccgg ccgccgtacc ggcgccgcga
     4141 acggctccac ggccgtgttc tccacgctgt tgaagacgat gaagacgttg ctgcgcggga
     4201 acggtgtgat gttgtcgccc gaaccgtgca tgcagttgca gtcgaaccag gtcgccgaac
     4261 cggccttgcc ggtgaacagc ttgatgccgt actccgacgc catggccgtc agcgcctcgt
     4321 cggacggggt gccggcgtcc tgcatctgca gcgacttctt gtagttgtcc ttcggggtgg
     4381 ctcccgcgca gccgaggaac gtccggtgcg accccggcat gatcatcaga ccgccgttgg
     4441 tgtcgtagtt ctcggtcagg gcgatcgaga cggacaccgt ccgcatgttc ggcagtccgt
     4501 cctcggcgtg ccaggtctcg aagtcggagt gccagtagaa gccggacgcg ccgaagcccg
     4561 gcttcacgtt gatccgcgac tggtggacgt agacgtccga gccgaggatc tgccgggccc
     4621 gcccgacgac ccgctcgtcc cgcaccagcc gcgcgaacag ctcactgatc cggtgcacct
     4681 cgaagaccga ccggatctcc tgcgacttcg gctcgacgat cgagcgctcg tcggcgcgga
     4741 tcgccggatc ggcgacgagc cgctccagct cctgccggta cacggcgacc tcgtccggcg
     4801 cgatcagctg ctcgacgggg aggaagccgt cgcgctcgta cgcctggagt tcggcgaggg
     4861 agacggggcc gggcgtgccg ggggagcccc agacgaccgg gtcctgacgc gggaccgaca
     4921 cctcggtggc cccacggctg gggtagaggt cggggacggt ggtggcagtg ttcatcgtgg
     4981 tggtcgtggt catggtgatg tcacacctcc tcgggctcgg tgagcagcgg gtagacgccg
     5041 ttctcgtcgt ggtcctcccg gccggtcacg ggcgggttga agacacagac gcagcggaag
     5101 tcctccttca cccgcagcgt gtgccgctcg tgcccgtcga ggaggtacat ggttccgggc
     5161 gtgatcgtgt aactccgccc ggtctcctgg tcggtgagct cggcctcgcc ctcgacgcac
     5221 acgacggcct cgacgtggtt ggcgtaccac atcgacgtct ccgtacccgc gtacagagtc
     5281 gtctcgtgca gggagaagcc gacccgctcc cgggcgagca cgatgcgctt gctctcccag
     5341 gtgccggacg ccgacttcac atggcggtcg gttccttcga tgtccttgaa cgaacggacg
     5401 atcacggtgg tgcgatgcct cctagttaga tggttcgccg tacggcgccg gacggtctcc
     5461 cgtgcggcgt cagacggtct cccgtacggc gcgggcgagg acgcgcaggc cctcgtccag
     5521 ctcgtcgggg gtgatggtga gggccggcag cagcttgacg acctcgctct ccgggccgga
     5581 cgtctcgatc agcagcccga gttcgaaggc ccgcttggcc acccgtcccg cgcgctcctt
     5641 gtcgtggaac tccatgcccc acaccaggcc gcggccccgg tactccttca cgtcggcgag
     5701 gttctcctcc gtgatggaga tgaacgcctg ctcgacctgc tcaccccgct tgcgggtctg
     5761 cttctccatg acggagccgt cggcccagta cgtctccagg gtggccgtcg ccgtcacgaa
     5821 cgccgggttg ttgccgcgga aggtgccgtt gtgctcgccc ggctcccaga tgtccagctc
     5881 cggcttgaac aggcagagcg acatcggcag cccgtagccg ctgatggact tcgagacggt
     5941 cacgatgtcg ggcgtgatac cggcctcctc gaacgagaag aaggcgcccg tacgaccgca
     6001 gcccatctgg atgtcgtcga cgatgagcag catgtcctgc cgtgcgcaca gatcggcgag
     6061 cgcgcgcagc cactccgggc gggcgacgtt gatgccgccc tcgccctgca cggtctccac
     6121 gatcaccgcg gcgggcttgt tcagccccga gccctggtcc tccaggagcc gctcgaacca
     6181 caggaagtcc tcgaccgtgc cgtcgaagta gttgtcgaac ggcatcgggg tgccgtgcac
     6241 cagcgggatc cccgccccgg cccgtttgaa ggcgttgccg gtgacggcga gcgagccgag
     6301 cgacatgccg tggaaggcgt tggtgaagga gacgaccgac tcccggccct tcaccttccg
     6361 tgccagcttc agcgccgact ccaccgcgtt ggtgccggtc ggcccgggga acatgacctt
     6421 gtacggcagg tcgcgcggcc gcagcaccag gttctggaag gactccagga acgcccgctt
     6481 ggcggtcgtc gacatgtcga gcccgtgggt gacgccgtcc cgttcgagat agtcgatgag
     6541 ggcgcgtttg agcacggggt tgttgtggcc gtagttgagt gaaccggcgc cggcgaagaa
     6601 gtccaggtac gtgtggccgt cctcgtcgaa catccggctg ccctgcgcgc ggtcgaagac
     6661 ggtgggccag ccgcggcagt agctgcgcac ctcggactcc agggtctcga agacgctcag
     6721 gtcgggctgg gtgatggtca cgtcgaatcg ctcctcggtg cgtggggggt gaggggaggg
     6781 agggggagtg gagaaggggg cgggtcctcc ggcgggcgga gaccccgggc gggcgggaca
     6841 tcccgggcgg gcgggaggtc ccgggcgggc ggcggcctca gggggacagc gggccgatgc
     6901 ggtggagcac ctcgggctgg tgcgggccgt ccgggaagtc ccccgcctcg aacaggaccg
     6961 tgcgctcgac gctcgcgccg tgccgttcgg cgtacgcggc gaacagtcgc tcggacgcgg
     7021 tgttgccggg cgtgatcgtc gtctcgacgg tggtcagggg gtgccgtcgc gcgacccggg
     7081 cggtgagccc gtccagcagc gcgcccgcga gcccgagccc ccggtgcgcc tcgtcgacgg
     7141 ccacctgcca caccagcagg gtgtccggtc gctccggccg cacatacccg gtcacgaacc
     7201 cgaccggctc cccggactcg tcccgcgcca ccgccgaggt gtccgcgaag tcccggcacc
     7261 acagcagata gctgtacgag gagttcaggt ccagggctcc ggagtcgccg gcgatgcgcc
     7321 acagcgacgc gccgtccgac acgtccgggc ggtcgatccg aatcccttcc gcgatttctg
     7381 tcagtggttc tgcttgtgcg gcagtcatgg ggcttgaatt tacccatggg aaattcgaat
     7441 tgcatcgccg cgcggggtta cgcatgggcc gttcacgtgt tatcacgcgg gcgtgaggaa
     7501 cctgcggtta cctggtcagt gtgggcggtt tcgcccgtaa attaccgcac aaaacgggcg
     7561 cattgtggag tcggtcacac gtacgtaacc ctcacgactt ctgcccgaat cggccccgcc
     7621 ggtgttcgcg aaacctttgc gtttaggtga ggggagagcg ggcagaagaa tacgggatcc
     7681 tgttctggga attccactgt attcctgccg gaaaagccga tcgcattccc ccgccggtgg
     7741 agcgggtttt ctacgcccag gcctccgccg ccgctcgccg ggccccttcc gggtcgacgg
     7801 cggcgccgaa ctccgccagc gccgcgccca gccccgcgag agcgcggtgc acgacgtccg
     7861 aggtcgcgtc ggggccgtag tggttcacgc ggatcatctc cgcggcgagc gccccgccgc
     7921 cggcggccag ggggagctcc ggatccgtgg cgagcgtctt ggcgaccagc tccgaggcgt
     7981 cgaccccggc gggtgcccgg agggtcgtgg ccaccggcgc ggcgtcggcc gcctcacgca
     8041 cgtacggctc caggcccccg cccagcgcga gcgcccccgc ccgggtcgcc gcggcggcgg
     8101 acgcgtgccg cgacatcacc gtgtcgagcc cctccgcctc gatgcgctcc agacaggcct
     8161 ccagggcgag catctccaac tgggcgggcg cgtgcaggag agcccgccgg ccgccgtcga
     8221 tccacctctc cttccagtcc aggagggaga ggtacgagcg gcgcggcgcc cgcgggttgg
     8281 ccgccatccg cgcccacgcc cgctcgctca cggagatcgc cgagaccccg gccggcccgc
     8341 ccatcgcctt ctgcgccccg atgacgcaca ggtcgacacc ccacgcgtcc ggcaggaccg
     8401 gctcggctcc gacggaggcc accgcgtcca ggtagaacag ggcgccgtgc gcgcggacga
     8461 cctcaccgat ctccgccacg gggttggtgt tgccggtggc cgcctccgcg tgcaccagcg
     8521 acacgaagtc gatcgacggg tgctcggcga aggcgtcgcg gatctgctcg gccgtcaccg
     8581 ccgtgtggaa cggcaccgcc aggtcgacga ccgtcgcccc gcagtcccgc agccagttcc
     8641 cgaaggtctg cccgtacggc ccggtgatca cgttcagcgc ggtcgtgccg ggcccggcgg
     8701 ccccgcggat cgcgccctcc agcggcagca gcgcctcgcc ctgggtgatc acgacgtcct
     8761 gctcggtgga gagcagccgg gccacccggt tctcgatcga ggcgaagtgc gacgcgctca
     8821 gcggggccag atccagaaac gggtgtgtca cggggtgctc tcttcacaga cggggtagat
     8881 ggtgtcgagg gtaccgacgg cacccgcgcg cccctcgccg gcgacatcgg aggtcagacg
     8941 gcccggtagc catcggaata gtttgagcaa gccaaacatt tccttatcat cggttgtctc
     9001 agttccccca caggaggtct ccccgtgaac tcggtccccg gacgccggac ccgcatcctg
     9061 gccgccacca ccgccgcggc cggactgctg ctcgtggccg gctgttcctc ggacgacggc
     9121 ggcagcggcg gcaccaagac cgcggccggc ggggtcgagc tggtgaaggc cggtcagctg
     9181 acgacctgca cccacctccc gtacccgccg ttccagtcgg agatcgacgg cgaggtgcag
     9241 ggcttcgacg tgtccctgat cgacctcgtc gccgacgacc tgggggtgaa gcaggagatc
     9301 ctcgacaccc ccttcgagaa cttcaagacg ggcgccttcc tgaactccgg cgagtgcgac
     9361 ctcgccgcgg ccggcatgac gatcaccgag gagcgcaaga agaacgtcga cttctccgac
     9421 ccgtacttcg aggccaccca ggcagtactc gccgccaaga aggccgacat cggctccttc
     9481 gcggacctca agggcgacgg ggactacaag gtcggcaccc aggcgcagac caccggcgag
     9541 gactacgcca agagccaggg cctcgacccg gtctccttcg agtcctccga cgccgtcctc
     9601 aacggcctgc gcaccggcca ggtcgacgcc gtcgtcatcg actacccggt ggtccagggc
     9661 tggctgaaga gcgcggacct cgccgacgcc tacacggtcg ccgagcagat caacaccggc
     9721 gaggagtacg gcttcacggt caagaagggc aacaccaagc tcctcgacgc cgtcaacaag
     9781 gccctcgcgg acgccaagtc cgacggcacg tacaagaagc tgtacgagca gtggatcggc
     9841 ccgtacgacg agagcgccgc ctccgcctcc ccgtccgcct catgagtgcg accgaccccg
     9901 acacaccgct ccagccgaag cgcaagggcc tcagccggag ccgcaggcgc gccctctcgc
     9961 ggggcgcgca gtacgcgctg ttcgtcgcgg ccgtcgtcgc cttcgcggtg tcggccgact
    10021 gggaccggct caagaaccag ttcgcccagt gggacatcgc cgagcagatg ttccccgacg
    10081 tcatcacgct ggcgctgaag aacaccgtcc tctacacgct gtccggcttc gtgttcggcc
    10141 tcgtcctcgg catggtcatc gcgctgatgc ggctgtcgtc ggtgggcccg taccgctggt
    10201 tcgccggcgt gtacatcgag atcttccggg gcctgcccgc gctgctgatc ttcatcttca
    10261 tcggcgtcgc cgtgccgctg gccttccccg gcacggagat ccccggcggc acgtacggca
    10321 aggtggcgct cgcgctcggt ctggtggccg ccgcgtacat ggcggagacg atccgcgcgg
    10381 gcatccaggc ggtccccaag ggcca
//
