LOCUS       NZ_CP080647            10477 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces akebiae strain MG28 chromosome, complete genome.
ACCESSION   NZ_CP080647
VERSION     NZ_CP080647.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces akebiae
  ORGANISM  Streptomyces akebiae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10257202)
  AUTHORS   Ping,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v. 3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019599145.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 09:26:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,127
            CDSs (total)                      :: 9,036
            Genes (coding)                    :: 8,727
            CDSs (with protein)               :: 8,727
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 142 of 309
            Pseudo Genes (incomplete)         :: 231 of 309
            Pseudo Genes (internal stop)      :: 20 of 309
            Pseudo Genes (multiple problems)  :: 77 of 309
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:57:32
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6631546
            Orig. end                         :: 6642023
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080647.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Mo Ping.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10477
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="22"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10477
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10477
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5477](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5477
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(94..1521)
                     /locus_tag="K1J60_RS28620"
                     /old_locus_tag="K1J60_28620"
     CDS             complement(94..1521)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /GO_process="GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bac_transf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1146: sugar transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065681.1"
                     /locus_tag="K1J60_RS28620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_28620"
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="WP_220648711.1"
                     /sec_met_domain="Bac_transf (E-value: 1.8e-62, bitscore:
                     209.0, seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTADSTISSSGTEPRNREFSTVSVMPPRGAVPRPPSSADGRRGRS
                     ASPMPLLVADGTAALLGALTLIGTQLHPLPSALLLGASLLLRPRHARPSEPGVLDELPA
                     VCRRTAVAWLAFAALVAAYRPDDALSTRTLLLGFTVQAATSCALRGTVHARRRAALARD
                     PRTALVIGPAATAQRVAAAVLRHPWCGVRPVGIVAERQNDAGGLPVLATGHEVERAVVQ
                     NGVQAVLAVHPSVRTQQQPLLSALAERGCVVWELDADSPSYATKDRLAGFSCRRLERAS
                     TPRGSAGKRALDILVSTLLLLLVSPLLLVCAVTLRISDGPGVLFRQERIGKDGKPFTLL
                     KFRTHRPLDEHEAATRWSVANEHEMRGFCRMLRRTSLDELPQLWNVLRGDMSLVGPRPE
                     RPYFVAEFSESHPGYAARHRVRTGITGLAQIHGLRGDTSIEDRFRFDNVYIDNWSLWQD
                     VSILLRTVGTLMRPTGS"
     gene            complement(1518..2696)
                     /locus_tag="K1J60_RS28625"
                     /old_locus_tag="K1J60_28625"
     CDS             complement(1518..2696)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005479504.1"
                     /locus_tag="K1J60_RS28625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_28625"
                     /product="glycosyltransferase"
                     /protein_id="WP_220648712.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 4.1e-35,
                     bitscore: 120.0, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHTSSTGPLARVLHLTQPVDGGVARVVTDLVQAQLTAGLAVTVAC
                     PDSALSTGLLQLGADVRHWPAKRSPGPSLVWEVRHLARVIDEVRPHLVHAHSAKAGLAG
                     RLAVRGRIPTVFQPHAWSFEATGGAVSILARAWERWGVRWASQVVCVSEAERMTGARAR
                     IDGRWSVIPNGVDVQRFRPGPVGPVRAGLTPLGEVDPAAPLVVCVGRLCRQKGQAVLLT
                     AWSSVARRVPGARLVLVGDGPDRERLRARAPKSVLFVGGVADVVPWYRAADLVVLPSRW
                     EGMALAPLEAMACGRPVVATDVGGARESLPPSLAARLLVPPENPALLAEAVTELLLDPP
                     LRASLGRQGLQYVLSSHDVRHSTEAVADVYRHLIGVPAPCVARTEHRESIQS"
     misc_feature    2738..2749
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.04"
                     /tool="antismash"
     gene            complement(2747..3895)
                     /locus_tag="K1J60_RS28630"
                     /old_locus_tag="K1J60_28630"
     CDS             complement(2747..3895)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056483.1"
                     /locus_tag="K1J60_RS28630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_28630"
                     /product="DUF3344 domain-containing protein"
                     /protein_id="WP_220648713.1"
                     /transl_table=11
                     /translation="MRNSLALLLRRTTVGALALAGMWAPTGALAVAPVTSSTQEAGRIP
                     FVQRYHALQHGGIVRAANVSITCRAPRATHRFLPSTTRSCPATRRDGTGVNGDFDMTYV
                     DVDKDPNTYNSSRAEVRVPEGSRVSYARLYWGGNLRVGEQKPPKDNGRVLIAEQGGEYK
                     EVLADTVVGHRVAHGADAFQASADVTRLVRRSGPGLYTVAQVNVAMGRSTAGAWGGWTL
                     VVAYENPKQPLRRLALWDGFDALKSGAAQEIRLRGTRFPANASGRAGLVTYDGDHGMTG
                     DTLTLTTTRTSSAPRPHRTVVTPLTNPANPRDDVLNSTISEPGTSEPQRVPTYTRTLGY
                     DSDVFDLGAGLRHGGDQLAFRVASQRDVIWTGVLFVAVDAQR"
     misc_feature    complement(4015..4032)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            4430..4708
                     /locus_tag="K1J60_RS28635"
                     /old_locus_tag="K1J60_28635"
     CDS             4430..4708
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136064.1"
                     /locus_tag="K1J60_RS28635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_28635"
                     /product="hypothetical protein"
                     /protein_id="WP_220648714.1"
                     /transl_table=11
                     /translation="MKSLKAAAVITGSLIIAGAAAPAFAQSATEVVHKSLSNTANTLNR
                     GPVQVQPLKGDVIDVKEKEKALLNTVDRTSTALGKGTPLLGGLSQQK"
     gene            5001..5477
                     /locus_tag="K1J60_RS28640"
                     /old_locus_tag="K1J60_28640"
     CDS             5001..5477
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545036.1"
                     /locus_tag="K1J60_RS28640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_28640"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_220648715.1"
                     /sec_met_domain="melC (E-value: 3.1e-42, bitscore: 143.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVPSFAVDPTGPTGRRRVLRGVFASATGVALAPYLVASEAAGPE
                     RAWFHETYRGRRIVGIADDAGPRGENELSGVWHVTVDGRPLHLMRRVDGSWMTMVDHYQ
                     SYPSPLAAARAAVDELGPNVRLGASGGTGSRRDDSEAPDSGEGTERGHRHGVRA"
     gene            5461..6327
                     /locus_tag="K1J60_RS28645"
                     /old_locus_tag="K1J60_28645"
     CDS             5461..6327
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="K1J60_RS28645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_28645"
                     /product="tyrosinase family protein"
                     /protein_id="WP_220648716.1"
                     /transl_table=11
                     /translation="MVYARRDVSTLTASERRRFVSALIEVKRRGEYDDFVRTHIQYFLP
                     DGENGRRTAHMAPSFLPWHRKFLLDLEQALRRVDESVTVPYWDWTRDRAPTAVPWTEDL
                     LGGTGRRTDRQVMTGPFAFRHGQWIIKEGITDAEYLMRDLGRSRDPIALPTAGDVEEAL
                     ADPVYDTAPWNSTSVKGFRNKLEGWGPGQGAVSWRNHNLVHRWVGGHMVSGASVNDPVF
                     WMHHAFVDLVWSRWQQRHRNTRYLPEKPPDPGGAQSGRVVARHERMPPWDVTPAELEDH
                     SSVYRYA"
     misc_feature    5770..5772
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6723..7651)
                     /locus_tag="K1J60_RS28650"
                     /old_locus_tag="K1J60_28650"
                     /pseudo=""
     CDS             complement(6723..7651)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129329.1"
                     /locus_tag="K1J60_RS28650"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_28650"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLDDYQSVATGIVGWSAVADDVEIVDFAEHFGERTPWRRPSPSTP
                     ACHAARTRCLPRLAPRPSPPAEAPDRLRHEQLGDRLRGRRGTRRDGVRHSQLPHPPVEL
                     TWALLLGLARGLGTENNPLREGGPWQRTSAPICTAPGSDCSSSAGSAAGWPGWAAPSAC
                     G"
     misc_feature    7807..7822
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.83"
                     /tool="antismash"
     tRNA            complement(8169..8239)
                     /anticodon=(pos:complement(6639752..6639754),aa:Arg,
                     seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="K1J60_RS28655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="K1J60_28655"
                     /product="tRNA-Arg"
     gene            complement(8169..8239)
                     /locus_tag="K1J60_RS28655"
                     /old_locus_tag="K1J60_28655"
     gene            complement(8328..9083)
                     /locus_tag="K1J60_RS28660"
                     /old_locus_tag="K1J60_28660"
     CDS             complement(8328..9083)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028876.1"
                     /locus_tag="K1J60_RS28660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_28660"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_220648717.1"
                     /transl_table=11
                     /translation="MPEQPPYLRIADELRRRIAERVWEPGDRLPSRAQIGHECGVGENV
                     VRRAQELLISQGVLEGRAGSGTYVAEPRQRVRVVRSSAREDSQESPFRADMRALGKQGD
                     WESRTDAKVPAPADIAARLGIGEGDLCVRTAYEFLADGRPVQLSTSWEPYDLTAGTLVV
                     LPEGGPHAGAGVVNRMAAIGITVSHAVEQPEPRQATAEEASLLGIQKAALVTHIRRTYY
                     SDQGRPVETADIVIPAAHCEIVYEIPINP"
     misc_feature    complement(9085..9099)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.6"
                     /tool="antismash"
     gene            9161..9442
                     /locus_tag="K1J60_RS28665"
                     /old_locus_tag="K1J60_28665"
     CDS             9161..9442
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950159.1"
                     /locus_tag="K1J60_RS28665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_28665"
                     /product="hypothetical protein"
                     /protein_id="WP_220648718.1"
                     /transl_table=11
                     /translation="MPASGSKAAAIAARLSAVGLSVRVEEHADRTSVEAELPDSLSVES
                     WREVLEAVAEADRFGLLAASVNGRTLWAAVVKKEVPTTGDVGGPGRQR"
     misc_feature    9269..9271
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    9551..9565
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 24.03"
                     /tool="antismash"
     gene            9980..10285
                     /locus_tag="K1J60_RS28670"
                     /old_locus_tag="K1J60_28670"
     CDS             9980..10285
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060538.1"
                     /locus_tag="K1J60_RS28670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_28670"
                     /product="hypothetical protein"
                     /protein_id="WP_220648719.1"
                     /transl_table=11
                     /translation="MTAQRGLRLLPWAGPDGKPCYLSTDDPEGYMSRLADHIESVQLGM
                     ARELLEEARQVPDGTPMNVHKLSLLAGQLAGALRDVIRVATSRGERLATPGHPEDE"
ORIGIN
        1 gcgccagcag ggcgagcacg gccaccacgg gaaggaccgg cgtcaggacg gtgcctcggc
       61 gcacgagcgg cgtcagtgcg aagctcatgg cgctcagctt cccgtcggac gcatgagcgt
      121 gcccacggtg cgcaacagaa tgctcacgtc ctgccacagc gaccagttgt cgatgtagac
      181 gttgtcgaac cggaagcggt cctcgatgga cgtgtcgccg cgcagtccat ggatctgggc
      241 gaggccggtg atgccggtcc gcacacggtg gcgagccgcg tagccggggt gggactcgct
      301 gaactctgcg acgaagtagg ggcgctcggg ccgaggtccg accagactca tgtcgccccg
      361 cagcacgttc cacagctggg ggagctcgtc gagcgaggtg cgccgcagca tgcggcagaa
      421 cccgcgcatc tcgtgttcgt tcgccacgct ccagcgggtc gccgcctcgt gttcgtcgag
      481 gggacggtgg gtgcggaact tcagcagcgt gaacggtttt ccgtccttgc cgatgcgctc
      541 ctggcggaac agcactcccg gcccgtcgct gatccgcagg gtcaccgcgc acaccagcaa
      601 caggggactg accagcagca gcagcagcgt cgacacgagg atgtcgagcg cccgcttgcc
      661 cgcgctgccg cgcggcgtcg aagctctttc cagacgtcgg caggagaacc cggccagtcg
      721 gtccttcgtc gcgtacgacg gagagtccgc gtccagctcc cagaccacgc agccccgctc
      781 ggccagcgca ctgagcagcg gttgctgttg ggtccgtacg gaggggtgga cggccagcac
      841 tgcttgtacg ccgttctgca cgaccgctct ctcgacctcg tgaccggttg ccagaacggg
      901 caggcccccg gcgtcgttct gccgttcggc cacgatcccc accggccgta ccccgcacca
      961 tgggtgccgc aacacggcgg cggccacccg ctgcgcggta gcggccggcc cgatgacgag
     1021 cgcggtgcga gggtcgcgcg ccagcgccgc tcgtcgtctc gcgtgcacgg tgccgcgtag
     1081 cgcgcagctc gtcgcggcct gcacggtgaa ccccagcaga agggtgcgcg tggacagcgc
     1141 gtcgtccggc cggtacgccg ccaccagtgc ggcgaacgcc agccaggcca ccgctgtacg
     1201 gcggcagacg gcgggcaact cgtcgaggac acccggctcc gacgggcgcg cgtgccgcgg
     1261 gcgcagcagc agcgacgctc ccagcagcaa ggcagacggg agcggatgga gttgagttcc
     1321 gatcagtgtc agggcaccca gcagggctgc cgtgccgtcc gcgacgagca ggggcatcgg
     1381 cgaggccgac cgccctcgcc gcccgtcggc ggaggacgga ggtctcggga cggcaccgcg
     1441 cggcggcatg accgagacgg ttgagaattc gcgattccgt ggctctgtgc cggaggagga
     1501 gatggtgcta tccgcggtca cgactggatg gactccctat gctccgtgcg tgcgacgcat
     1561 ggtgcgggca cgccgatcag atggcggtag acgtccgcga ccgcctcggt ggagtgccgc
     1621 acgtcgtgcg aggacaggac gtactggagc ccctggcggc cgagcgaggc gcgcagcggt
     1681 gggtcgagca gtagttcggt gacggcctcg gccagcagcg ccgggttttc gggcgggacc
     1741 agcagacggg cggcgagtga gggcggcagg ctctcgcggg caccgccgac atcggtggcg
     1801 acaaccggcc gaccgcaggc catcgcctcc agtggggcca gcgccatgcc ctcccaacgc
     1861 gacggcagga cgacgaggtc tgcggcccgg taccagggga cgacgtcggc gacaccaccc
     1921 acgaacagca cggatttcgg cgcgcgcgcc cggagccgct cgcggtccgg accgtcgccg
     1981 accaggacta gacgcgcgcc gggcaccctg cgggcgacgc tgctccatgc cgtcagcagg
     2041 acggcctggc ccttctgtcg gcacaaccgc cccacacaga cgacgagcgg cgcggcggga
     2101 tcgacctcgc ccaacggcgt cagtccagct cttacgggac cgacggggcc ggggcggaag
     2161 cgttgcacgt cgacgccgtt ggggatgacg ctccagcgcc cgtcgatcct ggcgcgcgcg
     2221 ccggtcatgc gttccgcctc gctcacgcac accacctggg aggcccagcg caccccccac
     2281 cgttcccacg cccgcgcgag gatcgagacg gccccgccgg tggcctcgaa agaccaggca
     2341 tgcggctgga acacggtggg gattctcccc cggacggcga gccgcccggc gagcccggcc
     2401 ttcgcgctgt gtgcgtgcac cagatgcggg cgtacttcat cgatcacgcg tgcgagatgt
     2461 cgtacctccc aaacgagtga cggccccggt gaccgcttcg cgggccagtg ccgtacgtcc
     2521 gcgcccagtt gaaggagccc tgtgctgagc gcgctgtcgg gacaggccac tgtgacggcc
     2581 agcccggccg tgagctgggc ctgcaccaag tccgtcacga cgcgggcgac cccgccgtcc
     2641 acgggctggg tgaggtgaag gactcgcgcc agagggccgg ttgatgacgt gtgcatgcgc
     2701 gatgtcctcg ctcacggtga cgctcgggac gggagaggaa aaggctttac cgttgggcgt
     2761 cgacggcgac gaagagcacg ccggtccaga tcacgtcccg ctgggaagcg actcggaagg
     2821 ccagttggtc gccgccgtgg cgcaggcccg cgccgagatc gaacacatcg gagtcatagc
     2881 cgagagtgcg ggtgtacgtc ggcacccgct gcggctcgga tgttcccggt tcgctgatcg
     2941 tggagttcag gacgtcgtca cggggattgg cggggttggt caggggggtg accacggtgc
     3001 ggtgcggccg aggggcggag gaagtgcggg tggtcgtgag ggtgagagtg tcgccggtca
     3061 tgccatggtc gccgtcgtac gtcaccaggc ctgcccggcc gctcgcgttc gcggggaaac
     3121 gcgttccgcg cagtcggatc tcctgcgccg caccggactt cagcgcgtcg aagccgtccc
     3181 agagtgcgag gcgccgcaac ggctgcttcg ggttctcgta cgccaccaca agcgtccagc
     3241 caccccacgc gccggccgtc gaccgtccca tcgccacatt gacctgtgcc acggtgtaca
     3301 ggcccggtcc gctcctccgg accagcctcg tgacgtccgc cgaggcctgg aaggcatccg
     3361 cgccgtgcgc cacccgatgg cctacgaccg tgtccgcgag gacctccttg tactccccgc
     3421 cctgttcggc gatcagcacc cggccgttgt ccttgggcgg cttctgctcg ccgacgcgca
     3481 ggttgccgcc ccagtacagc cgggcgtagc tgacccgtga gccctcgggc acacggacct
     3541 ccgctcggga ggagttgtag gtgttcgggt ccttgtcgac atcgacgtac gtcatgtcga
     3601 agtcgccgtt caccccggtc ccgtcccgcc gagtggctgg gcaggaacgc gtcgtggacg
     3661 gcaggaaccg gtgggtcgcc cgtggcgcac ggcaggtgat ggagacgttg gccgcgcgga
     3721 cgatcccgcc gtgctggagc gcgtggtacc gctggacgaa ggggatacgg cccgcctcct
     3781 gcgtcgacga tgtgacaggc gcgaccgcca gggcaccggt gggagcccac atcccggcga
     3841 gggcgagggc gcccaccgtt gtacggcgca gcaggagggc gagggaatta cgcatgactg
     3901 acggtgccct ttcgggaagg cggaggtgtc aggggcagga aggaacgagg tgcgtgctcc
     3961 ggggcacggc atcttcaaac acccttggga cgcgtcaaga gatacgcaaa actctagccg
     4021 ctatcaggca taaccgggtc aaacgtgagt aagtgtgtcg gatcggtgaa gtaggggctt
     4081 cccggacctg ttttccgcct cgcgagggca agcgggccca tggcttgagg cagccgcctc
     4141 cgcgcggtct gctcgcgtgg cccgtcgtgc ccacctgact gcgcgggtcg gacggctcgg
     4201 tggcgcggcg aagggcgccc tggaaggaca gcgcgggagc gcgacgctgt tgctttccgc
     4261 tcctgctcct ggcctgtcac ggcgcacggc gcaaggagtt aaatccttca ggggtttcat
     4321 atgagtactc ggcaatcttc cgcatgcggg tttccggacc ggtcggcgct cgttgtgcat
     4381 gacgtcagaa tccgccccgg ctacccatgt tgccgaagaa aggaatccaa tgaagtctct
     4441 gaaggctgcc gctgtcatca ccgggtctct gatcatcgcc ggtgccgccg cgcccgcgtt
     4501 cgcccagagc gccaccgagg tggtgcacaa gagcctcagc aacacggcga acacgctcaa
     4561 ccggggtccc gtccaggtgc agccgctgaa gggggacgtg atcgacgtca aggagaagga
     4621 gaaggcgctg ctgaacacgg tggatcgcac gtccaccgcg ctgggcaagg gcactccgct
     4681 gctgggcggc ctctcgcagc agaagtgaga acgcccccgg ccacgatgtc cgccgcgttt
     4741 tgatccggcg cttcaggcat ttcccggtgg ccgctccgaa gaggccgatt ccgcgtcgtg
     4801 cggtgtcggc ctctgcatcg tcgggacacg gtgtgatttc tgcggccaaa ttcactgcgc
     4861 aaaggtgaga ccgcatccgc gatgctgaag tttctgcttg gggatgtgcg ggattccggg
     4921 gggctcgtcg gacatgaagg agaattcatg gtgctggctc acgagttggg tgcgcagttg
     4981 tgtcgagggt ggaagaggcc atgactgttc cgtcattcgc cgtcgatccc acagggccca
     5041 ccggacggag gcgggtgctg aggggggtgt tcgcgtcggc gacaggggtg gcgctcgcac
     5101 cgtacctggt ggcatcggag gcggccgggc cggagagggc gtggttccac gagacgtatc
     5161 gcggtcggcg catcgtcggc atcgcggacg acgccgggcc gcgaggggag aacgaactct
     5221 ccggcgtgtg gcacgtcact gtggacgggc ggccgttgca tctcatgcgt cgcgtcgacg
     5281 gcagttggat gaccatggtc gaccactatc agtcgtatcc gtccccgctc gccgcggcgc
     5341 gcgcggctgt ggacgaactc ggacccaacg tgcggttggg ggcgtcgggt ggaaccggca
     5401 gtcgcaggga cgacagcgag gcgccggaca gcggcgaggg gaccgagagg ggtcaccgcc
     5461 atggtgtacg cgcgtaggga cgtcagcacg ctcaccgctt ccgagcggcg caggttcgtc
     5521 tccgcactga tcgaggtcaa acgccgaggt gagtacgacg acttcgtccg cacgcacatc
     5581 cagtacttcc tccccgacgg cgagaacggc cggcgcacgg cccacatggc gccctcgttc
     5641 ctgccctggc accgcaagtt cctgctggat ctggagcagg cgctgcgccg cgtggacgag
     5701 tcggtgaccg tgccgtactg ggactggacg cgcgaccgcg ccccgacggc cgtgccgtgg
     5761 accgaggact tactcggcgg caccgggcga cgcaccgacc ggcaggtcat gaccggcccc
     5821 ttcgccttcc gccatggcca gtggatcatc aaggagggca tcaccgacgc cgagtacctc
     5881 atgcgggacc tgggccgctc ccgggatccg atcgctctgc ccacggccgg ggacgtggaa
     5941 gaagccctcg cggacccggt ctacgacacg gccccctgga attccacgtc cgtcaaaggg
     6001 ttccgcaaca agctggaggg gtgggggccg gggcagggcg ctgtgtcctg gcgcaaccac
     6061 aacctggtcc accgatgggt cggcggccac atggtgagcg gcgcctccgt gaacgacccg
     6121 gtcttctgga tgcaccacgc cttcgtcgac ctggtgtggt cccgctggca gcagcggcac
     6181 cggaacaccc gctacctccc cgagaagccg cccgaccccg ggggcgccca gagcggccgg
     6241 gtggtcgcgc ggcacgagag gatgcccccg tgggatgtga ccccggcgga actggaggat
     6301 cacagttccg tctatcggta cgcgtagcaa gcacgaccat ctgcgggtgg ggccgagcga
     6361 aggcctgacc gtaccgggct tcgcacgcga cgccggacat tccgctgtgt gctgattgac
     6421 gcagggcgtc ggaagagtcc tgaccgggaa tctcgctttc atcgcgcacc ggggccggct
     6481 cggcaggaag gcaggacgag tgccgcctgc cgtcgcgggt ggaccggcgc cgttcgtccg
     6541 ctgcggggcg cggtcgtgcc ggcggcgcta cgcgatgtgc tcggcacacg ccgacccatt
     6601 gccggaggcc gaggcccgcc ggaaccggtg gatccggggc acctgcagcg ccagttcgct
     6661 ctcctggatg cctgaaggcg cgactgcagc gtggcggggc tcggtccggg tctccgggat
     6721 tctcagccga gcggtcgtac gggggagccc gccaggtata cctggatgtc ctcggcggtc
     6781 tggccgtagt acatggcgta gttggcccgg gagacatggc cgaggtgggg cgtggcgagg
     6841 agacgggggc cgtgcgcatc ggatggtcgg cggggagggg tgcgaggtcg aagacgtcag
     6901 ctccggcgag gcgcccctca tgcagggctg tgagcagggc gtcctggtcg tcgattgtga
     6961 ccgggaggtg ttgatcggat aggaggtcgg cttgaggcgg gcgagttccg gggcgccgag
     7021 gagacctcgg gtgcggtcgc tcagggagag atggacggag acggagtcgc gctcgcgagc
     7081 aactcctcct gggaagcggc cagatggacg cccacctcgt cggcgtaccc cttcgtcagg
     7141 tgctggctcc ccgccgtcac ccgcatgccg aaggcgcggc ccacccgggc cacccggccg
     7201 ccgatcctgc cgaggacgag cagtccgagc cgggcgccgt gcagatcggc gccgacgtcc
     7261 tctgccaggg accaccctcg cgcagagggt tgttctcggt gcccaggccc cgggcgaggc
     7321 cgagcagcag ggcccaggtc agctcgaccg gcgggtgggg gagctggctg tgccgtacac
     7381 cgtctcgccg tgtgcctcgg cggccgcgta gtcgatcacc gagttgctca tgccggaggc
     7441 gatcaggagc ttcagccggg ggagacggcc gaggggcgag gcggggaagg caacgcgttc
     7501 gcgcagcgtg acacgctggc gtactcggcg aggggcgtcg ccagggtgtc ctctccccga
     7561 agtgttcggc gaaatcgacg atctcgacat cgtcggcgac cgcggaccag ccgacgatcc
     7621 cggtcgcgac ggactggtag tcgtcgagca cggcgcagcg gagtctcggt cgcgtggtgt
     7681 tgccttccgg ccgggtaccg ggggcgtggt cgcgagactg ggggcgcgag gatgaccgga
     7741 ctccacccag cagagatctc cgtgatcaaa cctggctgtg cgacgagtca tcgggtgaca
     7801 ggcacaacca atgtctgtac gacatccacg gcgccctcac gtcgagagag ccgtggggcg
     7861 ttccagtaac tgatccggcc gattctcatc tcgtgaccac tgccccggcc tgcggctccc
     7921 cgtagccgtg cgcggtactg ctcccgtcgc agccgccatg ccccctccgg cgcacggacg
     7981 gctcccagcg ccgccaggtg tgttgcctgg gggtgtgatg ggccagaagt tgggtccaga
     8041 ggtgttcgag atcgtcgatc ccggagacgg ggaggcgccg accacggctc tcacgacggg
     8101 gaccgtagcg cgcgagagcc ttgcgctgaa cagcaaaaac gccctcccgg aggagggcgg
     8161 ggtgtgggcg cccccggcag gactcgaacc tgcggccaag cgcttagaag gcgctcggcg
     8221 tgttcggtcc cgggaaggct ctgagctggg cggtagctgg cgcgcgtgga ggcgtcctgg
     8281 gctccgtcga caggtattcg acacggggcg tcagaaccga ctggggccta cgggttgatg
     8341 gggatctcgt agacgatctc gcagtgtgcg gcgggaatga cgatgtccgc ggtctccacg
     8401 ggccgtcctt ggtcgctgta gtaggtccgt cggatgtgag tgacgagggc ggccttctgg
     8461 atgccgagga gggacgcttc ctcggcggtg gcctggcggg gctcgggctg ctcgacggcg
     8521 tggctgaccg tgatgccgat cgcggccatg cggttgacga cgccggcccc ggcgtgaggt
     8581 ccgccctcgg gaaggacgac gagggtaccg gcggtgaggt cgtagggttc ccagctcgtg
     8641 gagagctgca cgggccggcc gtcggcgaga aactcgtacg cggtgcggac gcacagatcg
     8701 ccctcgccga taccgagccg cgcggcgatg tccgccgggg ccggcacctt ggcgtcggtc
     8761 cggctctccc agtcgccttg cttgccgaga gccctcatgt cggcccggaa cggcgactct
     8821 tgggagtcct cgcgcgcgga ggaccgtacg acacggacgc gttggcgggg ctcggcgacg
     8881 taggtgccgg atccggcccg gccctccagt acgccctgtg agatcagcaa ctcttgcgcc
     8941 cggcggacga cgttctcgcc cacaccgcac tcgtggccga tctgggccct cgacggcagg
     9001 cggtctcccg gttcccagac ccgctcggcg atgcgccgtc gtagctcgtc ggcgatgcgg
     9061 agatagggcg gctgctcagg catgtggaaa atctagtcca ctagctctaa tctagttaac
     9121 tagcttcact gaaagtgatc gctggtgacg gaggctgcct gtgcccgctt cgggatcgaa
     9181 agcggcggcc atcgccgccc ggctgtccgc cgtcggactc tccgtgcgcg tggaggagca
     9241 cgccgaccgc acgtcggtcg aagcggagtt accggattcg ctctccgtcg agtcgtggag
     9301 agaggtcctg gaagcggtgg ccgaggcgga ccggttcggg ctcctcgccg ccagcgtgaa
     9361 cggccgcact ctctgggcgg cggtggtgaa gaaggaggtc cccacgacgg gcgatgtcgg
     9421 gggacctggc cgtcagcgat aggagctgat tcagcgtgct caaccgtatc cgccgcgccg
     9481 tctcccttgc tagggaccgg cgttcaccca agggccggca ccgccgcccg ttaactcgcg
     9541 ctcgttcact ggcccgtccg gcccccgcgg cgccccttga ggagcaggcg gcggctttgg
     9601 ggtatgccct ggaacgggct aggtaccgcc gctcgctgag gagcgaagac gtcgccctcg
     9661 tccgcccgta cgtcctggcg tgggagcaac gggcgcagca tccggcaggg gctgctgcgg
     9721 gacgccagtg gccggccgag gggcacccgg tcctgtgggg ggttggctga tgtcctcgca
     9781 ggaacagccg cgtagcccga acgtcgccta cagatcggca gcatgccgga tcgggacgca
     9841 ccgcgactgc gccgaatcct ctccggctgc ggcgccgacg ggtatcccgc tgatctacga
     9901 gacctgcgac tgcccgtgcc atgcagcggc aggcggcaca ccgacactcc ccaccaaggt
     9961 gaccgagagg cgcgcagaca tgacggcaca gagggggctc aggctcctgc cctgggcggg
    10021 accggacggc aagccctgct acctcagtac cgacgacccc gagggctaca tgtcccgcct
    10081 cgcggaccac atcgagtcag ttcagctggg tatggcaaga gagctcctgg aggaagcgcg
    10141 tcaggtcccc gatggcacgc ccatgaacgt gcacaagctg agcctcctcg cgggccaact
    10201 ggccggcgcc ctacgagacg tgatccgcgt ggcgacgagc cgcggagagc gcctggcaac
    10261 acctgggcac cctgaagacg aatgagcgct tgatcaccgc ccgggaactc cgggacaggg
    10321 ctacgcagta caagaggagc ctcgccatcg ggcggggctc ctgctcgtgt atgggaatcg
    10381 tgcacgtctc gctgccgggt ctgaagatgc acacggctcc ggaggccgct tcggtctcgt
    10441 ttattgctgc tggtcaaagg gcttttcgag ctaggga
//
