LOCUS       NZ_CP080647            11380 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces akebiae strain MG28 chromosome, complete genome.
ACCESSION   NZ_CP080647
VERSION     NZ_CP080647.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces akebiae
  ORGANISM  Streptomyces akebiae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10257202)
  AUTHORS   Ping,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v. 3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019599145.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 09:26:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,127
            CDSs (total)                      :: 9,036
            Genes (coding)                    :: 8,727
            CDSs (with protein)               :: 8,727
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 142 of 309
            Pseudo Genes (incomplete)         :: 231 of 309
            Pseudo Genes (internal stop)      :: 20 of 309
            Pseudo Genes (multiple problems)  :: 77 of 309
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:57:32
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2649999
            Orig. end                         :: 2661379
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080647.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Mo Ping.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11380
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="12"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11380
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11380
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6380](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6380)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(273..2030)
                     /locus_tag="K1J60_RS11425"
                     /old_locus_tag="K1J60_11425"
     CDS             complement(273..2030)
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512964.1"
                     /locus_tag="K1J60_RS11425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_11425"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_220646129.1"
                     /transl_table=11
                     /translation="MSTATATTAPSEADVPARGALRALLPALAAHRAMTARTCAAALLE
                     QGSLVALVTLAAHTVGTAVIEGRAPSAATVTALGALVLVRALTTWREMDLSHDLAYRVL
                     AVLRVRVFDGLARSAPARIAARRSGDLAATAMADVEALEFFYAHTTAQLLASATMFTGG
                     SLVLAVVEPWLLAAVLPVAALLAAAPFADARGRAARGGRTRTAAARLSADTVETVDGLR
                     ELLAFGRLAERRGTLARRGQQLGDAQRAEATWEAVAASVRDLLIVAAVLGVVTAAAQSV
                     TSGRLHGAWAPAAMALALSVLGPVAESARALSQAVALRAAAARVGAAVKAPALAPPPAA
                     PRPLPPGPLGVRLHGVRFDYGGQPVLDGVDLTVPPGGTLALVGVSGAGKSTIAHLLARF
                     WDPSEGTVQLVSAHGDLVDVRDVGDPELRRAVVLVGQETPLFHGTLAENLRLAAPEADD
                     DLLATAARRCGVDRIATMDTLVGERGATLSGGQRARIALARALLVRPRVLVLDESTAHL
                     DNTGDAQLAAALREKGRTTIVIAHRPATIRRADRIAVLETGRITEEGTWEDLTSRPGSA
                     LNRVLSTPS"
     gene            complement(2027..3766)
                     /locus_tag="K1J60_RS11430"
                     /old_locus_tag="K1J60_11430"
     CDS             complement(2027..3766)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004120.1"
                     /locus_tag="K1J60_RS11430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_11430"
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="WP_220646130.1"
                     /transl_table=11
                     /translation="MIVHPELRRAARGARRPLLASTFLQGAVTLTHLAQAALLALALAH
                     LARGDTGSLPWLLAAVLAVVAARAGLSVWQRRTATRAGARVRVTLRDELLTHLGRLGPA
                     HLTTARAGAVRTTLVDGVEGVDAYVSRYLPQLLITLTVPPLVLAALAVVEPWALLGLVP
                     ALLLALAGPRAWDRLLAARGKEHWDTYEGLGADYLEALQGMPALRAAGAVGRTRARLEE
                     RSAALHRATVAKLRVSLVDTAITDLAIQGGTAAAALLACWSAVTGSTTATGTYLVLLLA
                     SECFRPVRDLAREWHAGYLGVSAADGLAALRTAEPAVPDIGEAPAHWTTPPEVRFEDVR
                     FTYDGTRAPALDGVSFTAQAGRTTAIVGPSGAGKSTLLALLLRHHDPQEGRITLDGRPT
                     TEYTLDSLRRGIAVVSQETYLFHASIADNLRLARPGATDDDLTRAARVAGVHDEIAALP
                     DGYATVLGERGATLSGGQRQRLALARALLADAPVLVLDEATSAVDERREADILRELMNA
                     ASGRTVLVVAHRLAAVRHADRIVVLDEGRVDAVGEHAGLVEAGGVYARLVDAGRAHRGE
                     LAA"
     gene            complement(3763..4989)
                     /locus_tag="K1J60_RS11435"
                     /old_locus_tag="K1J60_11435"
     CDS             complement(3763..4989)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947323.1"
                     /locus_tag="K1J60_RS11435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_11435"
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="WP_259407682.1"
                     /transl_table=11
                     /translation="MEDLREDRPVDLVAALIRARSPEPPGPDTPAGGAVRAWPGPPRPL
                     PPAGPADLDLGDLLRHSLSAADDSGRLRPVSSAGALHPVDAELHVGAGCSLPPGRYGYD
                     PLRHRVHRLGRQPEGTPPGVTAELSVTSRRTVSHYGHRAWPLLLLDTGHAAAALFLAAR
                     ALGTDEPEVRLGGPAESPLATVHVPPPGGRGGPVHQEGGRGQAHPDGCARVLDPPGGPV
                     TPAELLARRSTPPPLPGAPSRDALLAVLTTAERAGTGTLREVLTTAARPGGGDLRWCAA
                     VGPPRPALMALSADGTTLRRYAAGEARPTLAAWAAGQEWIADAGAVLLAHGCPADADAA
                     HIRRAHLRAGFAVHLAHVTALRQGLAARPVGSWQQADLGAALGAAPGRDWVVHGLALGT
                     RHADEENAT"
     gene            complement(5001..6380)
                     /locus_tag="K1J60_RS11440"
                     /old_locus_tag="K1J60_11440"
     CDS             complement(5001..6380)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004118.1"
                     /locus_tag="K1J60_RS11440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_11440"
                     /product="YcaO-like family protein"
                     /protein_id="WP_220646131.1"
                     /sec_met_domain="YcaO (E-value: 7.5e-50, bitscore: 169.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLSLEALVDPVCGIVRKVEPVEHPTGAPPRYTALTAEISDARR
                     HGLWPADRVSLGTTFGDPGQARVAAIAEGVERYCGNRVPPPGHPDAPVRATATELADKG
                     LRLYGPGDLPAYADWQYAREKFPYRPLTPDTPALWTRGTELLPGGRAAEVWAPVALTHL
                     NWRQGELRELPRTHHLNYAGIATGQGLDDAVERGLLEIVERDALELWWHLDGPARGIDP
                     ASVPGLADDLAGSAFDVHIVEMYSEFAPCMAALVHDPRLGLYAAGFACKHDPAEAARKA
                     VLEAVHTWVFTQGLTDPDGWVFQAVEAGLLARGLYLDHRADGRYLDVCGEQFEHVRDLG
                     AHVQVWLDERMAAEARRFTEPALGTVSLDEVERGSRQRLDSTLHAGGHRVMTFDLTTED
                     VAETSLRVARVLVSGLLPNAPAAFGYFGCPRLAEAAVARGWRTSPPSAPEDFTLAPPPH
                     M"
     gene            complement(6377..7858)
                     /locus_tag="K1J60_RS11445"
                     /old_locus_tag="K1J60_11445"
     CDS             complement(6377..7858)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004117.1"
                     /locus_tag="K1J60_RS11445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_11445"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_220646132.1"
                     /transl_table=11
                     /translation="MTAFDATVEELATDVLLPAGDGPFPAVLLRTPYDRTRHRAEARGW
                     ARRGFAAVVQDVRGRFASPGEWHPYANEAADGAATVRWIRRQPWSDGRLVAVGASYAAH
                     CALVLALDVPEDARPDAVLAAVPALGLAETAREASGVERLLSRAGWWAAHGDRPDSDEN
                     ALDRALTADPGLLTHLPLIGLPERLGRGLPSWADLWRHRDRGRLIARAAHAAVPLLAVG
                     GHHDHFTEDTIALWHAWGGPSARLLLGPWGHGLGSTPGPDAGPAHRVNLGELYVRWARR
                     ALAGELAPGRRGSQGLGGSDLWLPAGTEGEPRTRRPRLLHGAAFTADPGRPVRSDDLTV
                     PADGPPDRCLLVTPPLPRPLDVVGPASVHLRATAHTPSADWAVRLVALTPEGVAEQLAV
                     GVVRRDEQAGHQAAFTVGLGRIARRLSAGIRLRLEIAGHHFPAHARNPHTGEDPVTVGR
                     LHVSRREIDPDSVVLSLPVLPSRPTPVDPAQEILR"
     gene            complement(7855..8709)
                     /locus_tag="K1J60_RS11450"
                     /old_locus_tag="K1J60_11450"
     CDS             complement(7855..8709)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048303.1"
                     /locus_tag="K1J60_RS11450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_11450"
                     /product="hypothetical protein"
                     /protein_id="WP_220646133.1"
                     /transl_table=11
                     /translation="MSRSRLVPGVEVVAVPGRGLAVRTQEGEFLSVRTESADEDVLLSR
                     LTGVTTGSVGEGLDRVVRAFGEAGYLAEGPQDSQWPAARRDIRILGDPVLTEPLAAQLA
                     ALGARPRTTDAVTASGGSTVGVEDLLDGDPSAVVWCLDGPVPDGLWGAADSLPDHGVAW
                     LRCHREGWQAYVEPVAAAPGDVTSAHVRSRRLAATPAHRELAAYWSGPRTSGAPVRLTP
                     PAATLLAALLADDLGRWATGAPDPAGLPARRRLRRVDLRTLTVTEHPVLPVPDVAPMPE
                     KDG"
     gene            complement(8801..8914)
                     /gene="amiA"
                     /locus_tag="K1J60_RS45750"
     CDS             complement(8801..8914)
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947327.1"
                     /locus_tag="K1J60_RS45750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="streptamidine family RiPP"
                     /protein_id="WP_240797855.1"
                     /transl_table=11
                     /translation="MDNEQIFTPIADQGQLAHASASHSNALVENPFDDNEE"
     misc_feature    complement(8971..8986)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 20.34"
                     /tool="antismash"
     gene            complement(8972..9793)
                     /locus_tag="K1J60_RS11455"
                     /old_locus_tag="K1J60_11455"
     CDS             complement(8972..9793)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004114.1"
                     /locus_tag="K1J60_RS11455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_11455"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_220646134.1"
                     /transl_table=11
                     /translation="MTESVGIHVEGVHFGYPGRPVLRGVDLTVRPGELTALIGLNGCGK
                     STLLRLAAGLLQPDEGRVRLGGDDLARLSRRATARRVALLHQSAPAVPGMTVRHLIRQG
                     RYAARGPLGMLREGDDPVVHRALRDVGVEEWADRDVDALSGGERQRVRLAMALAQDTRV
                     LLLDEPTTYLDLHHQLDVLQTVVRLRRERGLTVVMVLHDLAHAARFAERVVALRDGRVV
                     ADGAPGEVVTPGLLADVLKVAGRVGRDPEGGWPVCYPDHPLPDLEYENQIH"
     gene            complement(9814..10821)
                     /locus_tag="K1J60_RS11460"
                     /old_locus_tag="K1J60_11460"
     CDS             complement(9814..10821)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947329.1"
                     /locus_tag="K1J60_RS11460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_11460"
                     /product="iron ABC transporter permease"
                     /protein_id="WP_220646135.1"
                     /transl_table=11
                     /translation="MIRRFTVLAALAVVCAGVELVAGRGMSPGAAWDVLNGGGDPTERH
                     ILFQLRLPRLLVALVAGACLAVAGLVLQSALRNPLAGPEVTGVTPGAVLGAVTATALGL
                     AGWDSPLAVVLAACAGGCVGAALLWLLAGRGRGDPAHTAVHGVLVSAVLGGLTAMVLLV
                     EPGELGSVVQWLVGTTEGRVWQHWHLLWPWALVWGVAAWLLAGPLTLLRCGDDIALAAG
                     LSARPARTLALLCAVALTAGAVAAVGALGFVGLLVPHLAVAVFGADLRVSLPGAALVGA
                     VVVCGADAAAQLSSRLLAGALDSGRLTLPVGALTACLGAALLLVVVRRTPSRTF"
ORIGIN
        1 cccctgccac ccccgacggg cgggcgagtt ctcagcccgg tcgccaccct gccgcggctg
       61 ccgcccgggg caccgcgctc agcgagagcc tgtccgtcag caacgcggcg cacggcggat
      121 ccgccctcgg tctcagacac aggggcgcat acacgtgggg cccctccccg tgccgcccac
      181 cgtcgtggcg gcattcgtag ggaagaagtc ggggccgggt gccggcgcgc tcgagcctcc
      241 ccctgcccgg gcccccgggc ggcgtcccgt gctcaggagg gggtggagag tacgcggttg
      301 agcgcgctgc cggggcggct ggtgaggtcc tcccaggttc cctcttccgt gatgcggccc
      361 gtctcgagga ctgcgatgcg gtcggcacgt cggatcgtgg ccggccggtg ggcgatgaca
      421 atcgtggtgc ggcccttctc ccgcagcgcg gcggcgagct gggcgtcacc ggtgttgtcc
      481 agatgggcgg tggactcgtc gaggacgagg acgcgcgggc gtaccagcag cgcgcgggcg
      541 agggctatgc gggcccgctg tcctcccgag agcgttgcgc cgcgctcccc gacgagggtg
      601 tccatcgtgg cgatccggtc gacgccgcag cggcgcgccg ccgtggcgag caggtcgtcg
      661 tccgcttcgg gtgcggcgag ccgcaggttc tcggcgagag tgccgtggaa gaggggtgtc
      721 tcctgtccca cgagaacgac cgcccgccgc agttcggggt ctcccacgtc ccggacgtcc
      781 accaggtcgc cgtgggcgga gaccagctgg accgttccct cggaggggtc ccagaaccgg
      841 gccagcaggt gggcgatggt cgacttgccg gctcccgaga caccgaccag ggcgagtgtc
      901 ccacccgggg ggacggtcag gtcgacgccg tccagcacgg gctgtccgcc gtagtcgaac
      961 cgcacgccgt gcagccggac acccaacgga ccgggcggca ggggacgggg agcggcgggc
     1021 ggcggggcga gagcgggggc cttgacagcc gcgccgacgc gggcggccgc ggcccgcagt
     1081 gccacggcct ggctcagggc gcgcgccgac tccgcgaccg gaccgaggac ggacagggcc
     1141 agggccatcg ccgccggggc ccatgcgccg tgcagccgtc cggacgtcac ggactgcgcc
     1201 gccgcggtga ccacgccgag gacggcggcc acgatgagca ggtcgcggac cgatgcggcg
     1261 accgcctccc aggtggcctc ggcgcgctgg gcgtcgccca gctgctgtcc ccgcctcgcc
     1321 agcgtgccgc ggcgttccgc gagccggccg aaggcgagca gttcacgcag cccgtcgacg
     1381 gtttccacgg tgtcggcgga cagcctcgcc gcggccgtcc gggtgcggcc gccgcgtgcc
     1441 gcgcgcccgc gcgcgtcggc gaagggcgcc gcggcgagga gggccgcgac cgggagtacg
     1501 gccgccagca gccacggttc caccacggcg aggaccaggg aaccgccggt gaacatcgtt
     1561 gccgaggcca gcagttgggc ggtggtgtgg gcatagaaga actccagcgc ctccacgtcg
     1621 gccatggcgg tggcggcgag gtcgccgctg cgccgggccg cgatgcgggc gggcgcgctg
     1681 cgggcgagtc cgtcgaagac gcgcacccgc agtacggcca gcacccggta ggccaggtcg
     1741 tgcgagaggt ccatctcccg ccaggtcgtc agggcgcgga cgaggaccag ggcgccgagt
     1801 gcggtgacgg tggcggccga gggggctctc ccttcgatga cggcggtacc cacggtgtgc
     1861 gcggccaggg tgaccagcgc gacgagggag ccctgttcga ggagggcggc ggcgcaggtg
     1921 cgggccgtca tggcgcggtg tgccgcgagg gcgggaagaa gggcccgcag tgcgcctcgc
     1981 gcgggtacgt ccgcttcgga gggggcggtg gtggcggtgg cggtgctcat gcggcgagtt
     2041 ctcctcggtg ggcgcggccg gcgtcgacga gccgcgcgta gacgccgccg gcctcgacca
     2101 ggccggcgtg ctcgccgacg gcgtccaccc gtccttcgtc gaggacgacg atgcggtcgg
     2161 cgtgccggac ggcggccagc cggtgggcga ccaccaggac ggtccggccg ctcgcggcgt
     2221 tcatcagctc ccggaggatg tccgcttcgc ggcgctcgtc gaccgcgctc gtggcctcgt
     2281 cgaggaccag caccggggcg tccgcgagca gggcgcgcgc gagggcgaga cgctggcgct
     2341 ggccacccga gagggtggcg ccgcgctcac cgaggacggt ggcgtagccg tcggggaggg
     2401 cggctatctc gtcgtggact ccggcgacgc gtgccgcccg ggtcaggtcg tcgtccgtgg
     2461 cgccgggccg ggccaggcgc aggttgtcgg cgatgctggc gtgaaagagg tacgtctcct
     2521 gcgacacgac ggcgatgccc cgccgcagcg agtcgagggt gtactcggtc gtggggcgcc
     2581 cgtcgagtgt gatcctgccc tcctgcgggt cgtggtggcg caggagcagg gccagcagcg
     2641 tggatttgcc cgcgccggaa gggccgacga tcgccgtcgt ccgtcccgcc tgcgcggtga
     2701 aggagacgcc gtcgagggcc ggcgcccgtg tgccgtcgta ggtgaaccgg acgtcttcga
     2761 agcgcacttc gggcggggtc gtccagtggg cgggggcctc tccgatgtcg gggacggccg
     2821 gctcggcggt gcgcagggcc gcgaggccgt ccgcggccga cacgcccagg tatccggcgt
     2881 gccattcgcg ggccaggtcg cggaccggcc ggaagcactc ggaggccagc agcagcacca
     2941 gataggtgcc ggtcgccgtg gtggatccgg tgacggccga ccagcacgcc aggagcgcgg
     3001 ccgcggcggt gccgccctgg atggcgaggt cggtgatggc ggtgtcgacc agggacaccc
     3061 tcagtttggc gacggtcgcc cggtgcagcg cggcggaccg ctcctccagg cgtgcgcggg
     3121 tgcgcccgac tgcgccggcg gcacgcaggg cgggcatgcc ctggagcgcc tccaggtagt
     3181 cggcgccgag cccttcgtag gtgtcccagt gctccttgcc gcgggcggcc aggagccggt
     3241 cccaggcgcg cggaccggcc agggccagca gcagggcggg gacgaggccg aggagtgccc
     3301 acggttcgac cacggccagg gcggccagga ccaggggcgg cacggtgagg gtgatcagca
     3361 gctggggcag gtagcgggag acgtaggcgt cgacgccctc caccccgtcg acgagggtgg
     3421 tgcggacggc tccggccctc gcggtggtca ggtgggcggg ccccagccgg cccaggtggg
     3481 tgaggagttc gtcccgcagg gtcaccctga cccgggcccc ggcacgcgtg gcggtgcgcc
     3541 gctgccacac ggagagcccg gcgcgggcgg cgacgacggc gagcaccgcg gccagcagcc
     3601 acggaagcga accggtgtcg ccgcgggcca ggtgggcgag cgcgagggcc agcagtgcgg
     3661 cctgtgccag gtgggtcagg gtgacggcgc cctgcaggaa ggtggacgcg agcagggggc
     3721 gacgggcgcc cctggcagcg cggcgcagct cggggtggac gatcacgtgg cgttctcctc
     3781 gtcggcgtgg cgggtgccaa gggccaggcc gtggacgacc cagtcccggc ccggggcggc
     3841 gccgagggcg gcgccgaggt ccgcctgctg ccacgagccc acgggccgag ccgccagccc
     3901 ctggcgcagg gcggtgacat gggccaggtg gacggcgaag ccggcccgca ggtgggcgcg
     3961 gcggatgtgc gcggcgtcgg cgtccgcggg gcagccgtgg gcgagcagga cggcgcccgc
     4021 gtccgcgatc cactcctggc ccgcggccca ggccgcgagg gtcgggcggg cctctcccgc
     4081 cgcgtaccgc cgcagggtcg tgccgtcggc ggacagcgcc atcagggcgg gtcgcggcgg
     4141 acccaccgcg gcgcaccagc gcaggtcgcc gccgcccggc cgtgcggcgg tcgtgaggac
     4201 ctcccgcagc gtgcccgtgc ccgcccgttc cgcagtggtg aggaccgcca gcagcgcgtc
     4261 ccgggacggg gcgcctggca gaggcggcgg ggtactgcgg cgcgccagca gttcggcggg
     4321 ggtgaccggg ccccccggcg ggtccaggac gcgggcgcag ccgtccgggt gcgcctggcc
     4381 ccgcccgccc tcctggtgca ccgggccgcc ccgcccgccg ggcggcggga cgtgcacggt
     4441 ggccagtggg ctctcggcag gaccgcccag ccgtacctcg ggctcgtccg tgcccagcgc
     4501 gcgggccgcg aggaacaggg ccgcggcggc gtgccctgtg tccagcagca gcagcggcca
     4561 ggcgcggtgg ccgtagtggg agaccgtgcg ccgtgaggtg acggagagtt cggcggtcac
     4621 tccgggcggt gtgccctcgg gttgccgacc gaggcggtgg acgcggtggc gcagcgggtc
     4681 gtagccgtag cggccgggcg gcagggaaca gcccgcgccc acgtgcagtt cggcgtccac
     4741 cgggtgcagt gcgccggcgg aggacacggg cctgaggcgt ccggagtcgt ccgccgcgga
     4801 cagcgagtgg cgcagcaggt cccccaggtc gaggtcggcc gggccggccg gcgggagggg
     4861 gcggggcggt ccgggccagg cccggaccgc tcccccggcg ggtgtgtcgg gtccgggtgg
     4921 ctccggggag cgtgcgcgaa tgagggcggc gacgaggtcc accgggcggt cctcgcgaag
     4981 gtcttccacg ggtgcggccc tcacatgtgc ggcggaggcg ccagcgtgaa gtcctccggg
     5041 gcgctcggcg ggctcgtgcg ccaccctcgg gcgacggcgg cctcggcgag gcgcgggcag
     5101 ccgaagtagc cgaaggcggc aggggcgttg ggcagcagcc cggagacgag cacccgggcg
     5161 acgcgcaggg aggtctcggc cacgtcctcc gtggtgaggt cgaaggtcat gacacggtgg
     5221 ccgcccgcgt gcagcgtgct gtccagtcgc tgccggctgc cgcgttcgac ctcgtcgagg
     5281 gacacggtcc cgagcgccgg ctcggtgaac cgcctggcct cggcggccat cctctcgtcc
     5341 agccacacct gtacatgggc gcccaggtcc cgcacgtgct cgaactgctc gccgcacacg
     5401 tcgagatagc ggccgtcggc ccggtggtcc aggtacagcc cgcgggcgag cagcccggcc
     5461 tccacggcct ggaacaccca gccgtcggga tcggtcaggc cctgggtgaa cacccaggtg
     5521 tggacggctt ccaggacggc cttgcgggcg gcctcggccg ggtcgtgctt gcaggcgaac
     5581 ccggccgcgt acaggccgag gcgcgggtcg tgcacgagcg cggccatgca gggggcgaac
     5641 tccgagtaca tctccacgat gtggacgtcg aacgcggaac cggcgaggtc gtcggcgagg
     5701 ccgggcacac tcgccgggtc gatgccgcgg gccgggccgt ccagatgcca ccacagttcc
     5761 agggcgtccc gttcgacgat ctccagcagg ccgcgttcga cggcgtcgtc gagaccctgt
     5821 cccgtggcga tcccggcgta gttgaggtgg tgggtgcggg gcagctcgcg cagctcgccc
     5881 tggcgccagt tgaggtgggt gagcgcgacg ggcgcccaga cctccgccgc ccgtccgccg
     5941 ggcagcagtt cggtgccgcg cgtccacagg gccggggtgt cgggcgtgag ggggcgatag
     6001 gggaacttct cccgggcgta ctgccagtcg gcgtacgcgg gcaggtcccc cgggccgtag
     6061 agccgcagac ccttgtcggc cagttcggtg gcggtggccc gtacgggggc gtcggggtgg
     6121 ccgggcggtg ggacgcggtt gccgcagtac cgctccacgc cctctgctat cgcggcgaca
     6181 cgggcttggc cggggtcgcc gaaggtggtg ccgagggaga cccggtcggc gggccacagg
     6241 ccgtgtctgc gggcgtcgga tatctcggcc gtgagcgcgg tgtagcgggg cggggcgccc
     6301 gtggggtgct cgacgggctc gaccttgcgg acgatgccgc agacggggtc gacgagtgct
     6361 tccagcgaca gcgtcgtcat ctcaggatct cctgcgcggg gtcgacgggg gtggggcggg
     6421 acgggagcac gggcaggctc agcaccacgc tgtccgggtc gatctcgcgc cgggagacgt
     6481 gcagccggcc gaccgtcacc gggtcctccc cggtgtgcgg gttgcgggcg tgggccggga
     6541 aatggtgtcc ggcgatctcc aggcgcagcc ggatgccggc gctcagccga cgcgcgatcc
     6601 ggccgaggcc gacggtgaac gcggcttggt ggcctgcctg ttcgtcgcgc cggacgactc
     6661 cgacggcgag ttgctcggcg accccttccg gtgtgagggc gaccaggcga acggcccagt
     6721 cggcggacgg tgtgtgggcc gtggcccgca ggtgcacgct tgcggggccg accacgtcga
     6781 gcggacgcgg gagcggcggg gtgaccagca ggcagcggtc gggcgggccg tcggcgggga
     6841 cggtcagatc gtcggagcgg acgggccggc cgggatcggc ggtgaacgcg gccccgtgca
     6901 gcaaccgcgg ccgtcgggtg cgcggttcgc cctccgtacc ggcgggcagc cacagatcgg
     6961 acccgcccag cccctgtgag ccacggcggc ccggcgccag ttcgccggcc agggcgcggc
     7021 gggcccagcg cacgtacagc tcgccgaggt tcacccggtg ggccggaccg gcgtcggggc
     7081 cgggtgtgga accgaggccg tggccccagg ggcccagcag cagccgcgcc gagggtccgc
     7141 cccaggcgtg ccacagtgcg atggtgtcct cggtgaagtg gtcgtggtgg ccgcccaccg
     7201 cgagcaaggg cacggcggcg tgcgccgccc gcgcgatcag gcgtccgcgg tcgcggtgcc
     7261 gccacaggtc cgcccaggag ggcagcccgc ggcccagccg ttcggggagg ccgatgagcg
     7321 gcaggtgggt gagcagaccg gggtccgccg tgagggccct gtccagcgcg ttctcgtccg
     7381 agtccggtcg gtcgccgtgg gcggcccacc atccggcgcg ggagagcagc cgctccacgc
     7441 cggacgcctc gcgtgccgtc tccgccaggc cgagggcggg tacggccgcg aggacggcgt
     7501 cgggccgggc gtcctcgggc acgtcgagag cgaggacgag ggcgcagtgg gccgcgtagg
     7561 aggcacctac ggccaccagt cggccgtcgc tccacggctg ccgccggatc cagcggacgg
     7621 tcgccgcgcc gtcggcggct tcgttcgcgt aggggtgcca ctcccccggt gacgcgaacc
     7681 ggccgcgcac gtcctggacg acggcggcga atccgcggcg ggcccagccg cgcgcctcgg
     7741 cccggtgccg ggtccggtcg tagggagtgc gcaacaggac ggccgggaag ggaccgtcac
     7801 cggccgggag gagcacgtcg gtggcgagtt cctccacggt cgcgtcgaac gctgtcatcc
     7861 gtccttctcc ggcatcggtg ccacgtcggg caccgggagg acggggtgct ccgtgaccgt
     7921 cagcgtgcgc aggtccacgc ggcggagccg acgacgggcc gggagaccgg ccgggtccgg
     7981 ggcgccggtg gcccaccggc cgagatcgtc cgcgagcagc gccgcgagca gcgtggcggc
     8041 gggcggggtg agccggaccg gcgcgccgga ggtacgaggt ccgctccagt acgcggccag
     8101 ttcgcggtgg gccggggtgg cggcgagccg gcgggaccgg acgtgcgccg aggtgacgtc
     8161 tccgggagcg gcggccaccg gctcgacgta cgcctgccag ccctctcggt ggcagcgcag
     8221 ccacgcgacg ccgtggtcgg gcaggctgtc ggcggcgccc cacagcccgt ccggcacggg
     8281 tccgtcgagg caccacacga ccgcggaggg gtctccgtcg agcaggtcct cgacgcccac
     8341 cgttgatccg cccgaggcgg tgacggcgtc agtcgtgcga ggccgggcgc ccagggcggc
     8401 cagctgcgcg gcgagcggtt cggtcagtac cgggtcgccg aggatccgga tgtcccggcg
     8461 agcggcgggc cactgcgagt cctgcggccc ttcggccaga tagcccgcct ccccgaacgc
     8521 ccgcaccaca cgatcgagtc cctcgcccac cgagcccgtt gtcacgccgg tgagccggga
     8581 gagcagaacg tcctcgtcgg cactctccgt cctgacgctg aggaactccc cttcctgggt
     8641 ccggacggcg agccctcgcc cgggcacggc gacgacctct acaccgggga cgagtcggct
     8701 gcgggacacg gcgatcctcc tgacgaaagg tgggggatgg gcgtgggccc gcccggaagg
     8761 caacgggcgg gcccacggtc gagggtggcc ggaccctcgc ttactcctcg ttgtcgtcga
     8821 aggggttctc gacgagcgcg ttggagtggg aggccgaagc gtgggccagc tgcccctggt
     8881 cggcgatcgg cgtgaagatc tgctcgttgt ccatgaactc tccttgtacg tccgggagtt
     8941 acggcgttcg agtgagcgcc gcgacaacac gttaatgaat ctgattttca tattctagat
     9001 ctgggagggg gtgatctggg taacacaccg gccagcctcc ctcggggtcc cggccgaccc
     9061 gtcccgccac cttcagcacg tccgccagca atcccggggt caccacctcg cccggcgcgc
     9121 cgtcggccac cacacggccg tcgcgcaggg cgacgacgcg ttcggcgaac cgggccgcgt
     9181 gggccaggtc gtgcagcacc atcaccacgg tgagcccgcg ttcccggcgc aggcgaacca
     9241 ccgtctgcag cacgtcgagc tggtggtgca gatccaggta ggtggtcggc tcgtcgagca
     9301 gcaggacacg cgtgtcctgg gcgagcgcca tcgcgagccg gacccgctgc cgctcgcctc
     9361 cggacagcgc gtcgacgtca cggtcggccc actcctccac cccgacgtcc cgcaacgccc
     9421 ggtgcacgac ggggtcgtcg ccctcgcgca gcatgcccag cggtccgcgc gccgcgtacc
     9481 ggccctggcg gatgaggtga cgcaccgtca tgcccgggac ggccggagcg gactggtgca
     9541 gcagggccac cctccgggcc gtggcgcgcc gggacagccg ggcgaggtcg tcccctccta
     9601 gccgaacacg cccttcgtcc ggttgcagca ggccggcggc cagccgcagc agggtcgact
     9661 tgccgcagcc gttcagcccg atgagggcgg tcagttcgcc cggccggacg gtcaggtcga
     9721 cgccccgcag tacaggtcgc ccgggatagc cgaagtggac cccctcgacg tggatcccca
     9781 cggactcggt catgctctgt cctcgattcg acgtcagaac gtacggctcg gcgtgcggcg
     9841 cacgacgacc agcagcaacg cggcgccgag gcaggcggtg agcgccccga ccggcagcgt
     9901 gagccgtccg gagtccagcg cccccgccag cagccgcgag gacagctgcg ccgccgcgtc
     9961 cgccccgcac accaccaccg cgcccaccag ggcggcgccg ggcaggctca cccgcagatc
    10021 cgcaccgaag acggccacgg ccaggtgcgg aacgagcagt cccacgaagc ccagcgcccc
    10081 gacggcggcc accgcgcccg ccgtcagcgc caccgcgcac agcagggcca gggtccgggc
    10141 cgggcgagcc gagaggccgg cggccaaggc gatgtcgtcc ccgcagcgca gcagggtcaa
    10201 cggcccggcc agcaaccagg ccgcgactcc ccagacgagg gcccacggcc acagcaggtg
    10261 ccagtgctgc cacacccggc cctccgtggt gcccaccagc cactggacga cactgccgag
    10321 ttcgcccggc tccaccagga gcaccatcgc ggtgagcccg ccgagcaccg ccgacaccag
    10381 caccccgtgc acggcggtat gcgcggggtc accgcgcccg cggccggcga gcagccacag
    10441 cagcgcggct ccgacacacc cgcccgcgca cgcggcgagg accacagcca ggggcgagtc
    10501 ccacccggcg agtcccaggg cggtcgcggt gacggctccg agcaccgccc ccggggtcac
    10561 gcccgtcacc tccggcccgg cgaggggatt gcgcagggcg gactgcagga ccagccccgc
    10621 gacggcgaga caggcaccgg cgacgagagc caccagcagc ctgggcaggc gcagttggaa
    10681 caggatgtgg cgctccgtcg ggtcaccgcc gccgttcagg acgtcccagg ccgcgcccgg
    10741 ggacatgccg cgcccggcca ccagttccac accggcgcac acgacggcca gcgccgcgag
    10801 aaccgtgaat cgccggatca ccgtacgtcc tccgttctgg tcgcccccga agagggcggg
    10861 cagccatcgt cgatccggcc gcgcccgggc gcgtctgcgc tcacgtcgtc cgacgccctg
    10921 gctcgggaga ggtcgtccgc ggcaccggaa gccgccttcc tcggtccggg cagcggctcg
    10981 cgcaccgcgg ccgcggcggc cggcctgcgc cgggccgccc ggccgcgacc gcccgaccgc
    11041 atcagcgcga ccccggccgg cacccccagc agggccgtcc acgcacctgc cggtgtctcc
    11101 acgggcgcca gcgccagccg agccgggagg tcggcgacga ccacggcgac cgcgccccag
    11161 actgcggacc acggaagcca gcgcaccgcc cccgccgcgg ggtcgaacca ccgggccagg
    11221 tgcggggcga ggaagcccac ccacgcgacg ggcccgcaca cggccgtcac cggggcgatc
    11281 agcaccaccg cgatggccag tgccgccagc cgggcgcgct gggcgcgcac acccagcgcc
    11341 tcggcgtcct cgtcgcccag ccgcatcacc gacagcaccg
//
