LOCUS       NZ_CP080647            11422 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces akebiae strain MG28 chromosome, complete genome.
ACCESSION   NZ_CP080647
VERSION     NZ_CP080647.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces akebiae
  ORGANISM  Streptomyces akebiae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10257202)
  AUTHORS   Ping,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v. 3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019599145.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 09:26:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,127
            CDSs (total)                      :: 9,036
            Genes (coding)                    :: 8,727
            CDSs (with protein)               :: 8,727
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 142 of 309
            Pseudo Genes (incomplete)         :: 231 of 309
            Pseudo Genes (internal stop)      :: 20 of 309
            Pseudo Genes (multiple problems)  :: 77 of 309
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:57:32
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2458048
            Orig. end                         :: 2469470
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080647.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Mo Ping.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11422
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="10"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11422
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11422
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6422](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6422
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            41..550
                     /locus_tag="K1J60_RS10705"
                     /old_locus_tag="K1J60_10705"
     CDS             41..550
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K1J60_RS10705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="K1J60_10705"
                     /product="hypothetical protein"
                     /protein_id="WP_220646009.1"
                     /transl_table=11
                     /translation="MNARISVVDEDVVREVTRIVVSEVAPEEMALFGMNSRAYFRDPAR
                     TLEEAAGDGGGSKESLMGAGGAEVVVMLTPFALAMVQGALSSFIGAFAANLADGGGTAV
                     RRWLRGLFGRSDGENEAADPDSALTPDQVEWLREEAYRTLRRAELGDSQARLLADALAG
                     AARVRR"
     gene            552..3137
                     /locus_tag="K1J60_RS10710"
                     /old_locus_tag="K1J60_10710"
     CDS             552..3137
                     /EC_number="3.4.24.-"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013594.5"
                     /locus_tag="K1J60_RS10710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_10710"
                     /product="M48 family metalloprotease"
                     /protein_id="WP_220646010.1"
                     /transl_table=11
                     /translation="MTTPPAPPTRPAPPDPFALPSGAKFRFALVVTALLGTMAFIHNLL
                     YFTFSPDRGRAVALYQRCASAPADRPATADALSYTQGFLSCIAPYERGKAWWVLGGILL
                     LVVTATAVYLLLPSFLTRYGQLEPLSPHDPDQAPLLARLHSLSQEAGLSRPPEFLVSGL
                     TDRRVDAYTFGRSGRHRVALAQGTVRAFGLAPERARAVVLHELAHLRNKDVDVFYLTIA
                     LALAFVPTGLLPLAVALIGTPASGVLSVTWRSLALVALVWITCVSVLRIREYGADVRAA
                     SWGAGPGLLDVVGTESGRAVPWWRRGPLALHPSAAARAAEIVRPRELFRVRFTECFAAG
                     LAVSVAASGLLTLLWLVVNRLDALDARWTTVLLCAPAALAVMGLGVWRAALFGGGTRAV
                     LLPGLGLGLGLVVGGPLAPQNGIVLTGRPLAPGPAALLSSLALCSLMVALSAWIARSAA
                     LWHATGEQPPGKAWQAGLSATALPFAVLLSGWMLLYDTGDGLGPVHDSARDLYGVVSAS
                     APVGPFWLWALVEHPLTLLFTRWTPLVVALVALWLFPLATLWHRRPPYDTAWVGQVLTT
                     SAGTAGVFAWLQILERVVLRQGVSQAVRSQDGFVLAFAYWQIAAAMLFQGVAAAVTVRS
                     HRHDRLVIPLALLTAFLTGCLLTLVFFAGVVLAGCADGLALVPGPCSLDLPLPLVRDTL
                     LRILAGGFLCALVGVAVPVTVGRLRGERPPVAPGTPVRHALRARPNRWLAVGVAALPAS
                     LVLGFALLVGPIGQPSDVRPADSSSAAACRAFDGLLGSMGSLGPADANARLHEAVRLAL
                     LAGDTSLATDFQGLFAAAGDQDAERFGALTDRIGDRCADEGAPLGNPPR"
     gene            3297..3905
                     /locus_tag="K1J60_RS10715"
                     /old_locus_tag="K1J60_10715"
     CDS             3297..3905
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481898.1"
                     /locus_tag="K1J60_RS10715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_10715"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_220646011.1"
                     /transl_table=11
                     /translation="MRRLNGSLIASLALVVTVGALAFPVWSYADRSGTAQANMAASTVN
                     TQWGPLTAADRDLIIRVRLAGLWELPAAEKAMARSSSPEVKEAADHLIVGHKDLDERVR
                     NVASQLGVELPNVPNEQQVGFLAQMDNATDDQFDRVWANLLRSAHGKIFPAIGAIRNQT
                     ENTLVRQLASDTNQTVLDHITMLEKTNQVDFDAIANGTI"
     misc_feature    complement(3950..3962)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.5"
                     /tool="antismash"
     gene            4038..4844
                     /locus_tag="K1J60_RS10720"
                     /old_locus_tag="K1J60_10720"
     CDS             4038..4844
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481896.1"
                     /locus_tag="K1J60_RS10720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_10720"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_220646012.1"
                     /transl_table=11
                     /translation="MRRSKGSVLVALAIAGTLTAVAYPIFYSYPNRNATAAAALTGDTV
                     TTQWGPLTPADRDLLIRVRLAGLWELPAGQQALERSGSQSVREAADHLIVGHTDLDKRS
                     RVIAAKLGVELPNQPTAEQQGWLDQMTASKTEAEYNKTWANLLRAAHGKIFPAIASVRN
                     STRNTLIRQLASDANQTVLDHITILEKTGEVDFATIADNSVSGTASPTGPPPPVPGQVP
                     PAAPPAVPSTNPDVQSKPSPFESGNVPTGRPDPEEINKDQGQPQVQ"
     gene            5001..6422
                     /locus_tag="K1J60_RS10725"
                     /old_locus_tag="K1J60_10725"
     CDS             5001..6422
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432057.1"
                     /locus_tag="K1J60_RS10725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_10725"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_220651409.1"
                     /sec_met_domain="DUF692 (E-value: 2.1e-96, bitscore: 321.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQIAQLGTGIGWRPEIADAVERMAGADGGVDWVEVVAENVCPGH
                     IAESLLRLRERGVTVVPHGVSLGLGGADRPDEQRLRSLAERAEALGAPLVTEHIAFVRA
                     GGALTASPLLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALINWPGEEM
                     TEGQFLYDLVDRTGVRLLIDVANLHTNHVNRGEDPAKALDELPVEAIAYVHVAGGFERD
                     GVWHDSHAHPVPQQVLDVLADLASRIAPPGVLLERDDNFPEPGELERELTAIRGVLEKV
                     EVRGPGKDVREPEKGARETDRGERVPGRKAATARAKAVAETEPAAEAVPVTEAEPAARQ
                     RLGLAQAALLSALVAGTPVPEGFDRVRLGVQARALAAKRADVVAKVAPELPEILGSSYR
                     PTFVAYAQGHPMTDGYRRDALSFAEHVLLTGRPEDADVRREVRRWWLERSGPAPLSQRP
                     TARLARATRRVLLRR"
     gene            6674..7465
                     /locus_tag="K1J60_RS10730"
                     /old_locus_tag="K1J60_10730"
     CDS             6674..7465
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481894.1"
                     /locus_tag="K1J60_RS10730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_10730"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_220646013.1"
                     /transl_table=11
                     /translation="MRTTNGRGGLFSGTGLIVAGLTATLVALLFPIWSYADRSGTGVSV
                     LNASTVTTQYGPLSALDRDFVTKVRLAGLWELPAGQQAQTKGSTLAVRTAGEHLVEGHT
                     FLDARVRKVAARLALPLPNQPSDQQKAWLDTLTAAQGETYDREFANILRLAHGKVFSVV
                     AQVRATTRNSLVRDLADDANTTVLDHIKVLEATGFVDYDAIARDAASASAPPLTNSPTP
                     PTPLDDPGSPVPVTPSPTPTRPDPSPTYALPPAANGPADDD"
     gene            7635..8402
                     /locus_tag="K1J60_RS10735"
                     /old_locus_tag="K1J60_10735"
     CDS             7635..8402
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481893.1"
                     /locus_tag="K1J60_RS10735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_10735"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_220646014.1"
                     /transl_table=11
                     /translation="MIWVLFLLLAWAAAGVSCARLCLTSMRAAAADSDVHVDHAHHCHR
                     LTLYEAAFLSGGPARVADLTLVSMAGRRGLLLAHTGWVTVVDPDGRDDMERSVIGAIGP
                     GGQSRIAPVRRTAAAADPVRRLSDRLVAAGLAVPYAARTAVADAVTQVRGAALGVCGLG
                     ALALMLPSQGYGEHHVLVALWFALPLVLTLSCLVIVRVDRHPGTRWATPEGVRVLRTVV
                     GRGGGDLADLVSVAVWGVGAVGSAELRAAFGQR"
     misc_feature    complement(8420..8435)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.83"
                     /tool="antismash"
     misc_feature    complement(8451..8464)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 20.6"
                     /tool="antismash"
     gene            8493..10091
                     /locus_tag="K1J60_RS10740"
                     /old_locus_tag="K1J60_10740"
     CDS             8493..10091
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (104-480). E-value:
                     6.5e-23. Score: 73.5. Matches aSDomain:
                     nrpspksdomains_K1J60_RS10740_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481892.1"
                     /locus_tag="K1J60_RS10740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_10740"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_220646015.1"
                     /sec_met_domain="PF00561 (E-value: 2e-26, bitscore: 92.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAVLYGTVGAVVLSGLGAAPAEGDMSRTAAQSVALAAERAADE
                     GIRFGKCPAAEGLPDGVRCGTVEVPLDYARPDGRQISLTVSRIAAKGKDAKGRKVARQG
                     ALVFNPGGPGASGMYFPLAGYLPGWKRIGAAYDLVGYAPRGVGRSAPLSCQDPKRLHRG
                     PAPAPTHPSETYKKERVARAKAYARGCARRGGSALRHYHSLNNARDLDVLRAALGEPRL
                     TFMGASYGTYIGALYAALFPSHVRRMVFDSAVNPAPEQIWYRNNLDQSAAFEGRWADFR
                     AWVARHHRVYGLGDTPQKVLRSYERAAARLAARPAAGKVGPGQLQGAFLQAAYHDDHWP
                     QRALALSAYLRGDPKPLVRIAGPYPEAAVEQENAKAVYTAVECNDAPWPTRWSVWDRDN
                     TRLARTAPFETWDNVWTNLPCAYWSGPRQRPLDVRTGPGELPPTLILAAERDAAAPYDG
                     AIELHRRLWGSVLVTERDAGNHGVAGGSNTCVNGYLEAYLLQGRAPVRRAACAGRPEPS
                     AARREAVGELRVVGS"
     aSDomain        8805..9932
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K1J60_RS10740_Abhydrolase_1.1"
                     /evalue="6.50E-23"
                     /label="K1J60_RS10740_Abhydrolase_1.1"
                     /locus_tag="K1J60_RS10740"
                     /protein_end="480"
                     /protein_start="104"
                     /score="73.5"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASGMYFPLAGYLPGWKRIGAAYDLVGYAPRGVGRS
                     APLSCQDPKRLHRGPAPAPTHPSETYKKERVARAKAYARGCARRGGSALRHYHSLNNAR
                     DLDVLRAALGEPRLTFMGASYGTYIGALYAALFPSHVRRMVFDSAVNPAPEQIWYRNNL
                     DQSAAFEGRWADFRAWVARHHRVYGLGDTPQKVLRSYERAAARLAARPAAGKVGPGQLQ
                     GAFLQAAYHDDHWPQRALALSAYLRGDPKPLVRIAGPYPEAAVEQENAKAVYTAVECND
                     APWPTRWSVWDRDNTRLARTAPFETWDNVWTNLPCAYWSGPRQRPLDVRTGPGELPPTL
                     ILAAERDAAAPYDGAIELHRRLWGSVLVTERDAGNH"
     gene            complement(10122..10853)
                     /gene="hemQ"
                     /locus_tag="K1J60_RS10745"
                     /old_locus_tag="K1J60_10745"
     CDS             complement(10122..10853)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="K1J60_RS10745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K1J60_10745"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_220646016.1"
                     /transl_table=11
                     /translation="MSDDAPTTESGRIPNKGKLAKDLNEVIRYTLWSVFRLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDVSGLRADADVMIWWHAETSDQLQEAYNLFRRTR
                     LGRALAPVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARMHVR
                     EEVPFYTGRRKSVAELVAGLA"
     misc_feature    complement(10848..10863)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.71"
                     /tool="antismash"
     misc_feature    10851..10866
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.49"
                     /tool="antismash"
ORIGIN
        1 cagatcaact agcccatgtt cctgaacaaa gggggcggcc atgaatgcgc ggatatccgt
       61 cgtggatgaa gatgtggtgc gcgaggtgac ccggatcgtg gtctccgaag tcgcgcccga
      121 ggaaatggcc ctgttcggaa tgaacagccg ggcctatttc cgggaccctg ccaggacgct
      181 ggaggaggcg gccggcgacg gcggcgggtc gaaggagagc ctgatggggg ccggcggcgc
      241 cgaggtcgtc gtgatgctga cccccttcgc cctcgcgatg gtgcaaggcg cgctctcctc
      301 gttcatcgga gccttcgccg cgaacctcgc cgacggcggc ggcaccgccg ttcggcggtg
      361 gctccgcggc ctgttcgggc ggtcggacgg cgagaacgag gccgccgacc cggacagcgc
      421 actgacgccg gaccaggtgg agtggctgcg cgaggaggcc taccgcaccc tgcggcgggc
      481 cgaactcggg gattcccagg cccggttgct ggcagacgca ctcgcaggcg cggcccgcgt
      541 ccgccggtga cgtgaccacg ccccctgcgc cgcccacccg gcccgcccct cccgacccgt
      601 tcgcgctgcc cagcggcgcc aagttccgct tcgccctcgt cgtcaccgcg ctgctgggga
      661 cgatggcctt catccacaac ctgctctact tcacgttctc acccgaccgt ggacgggcgg
      721 tggccctcta ccagcgctgc gcgtcggccc cggcggaccg gcccgccacg gcggacgccc
      781 tctcctacac ccagggcttc ctgtcctgca tcgccccgta cgagcggggg aaggcgtggt
      841 gggtgctcgg cgggatcctg ctgctcgtgg tgacggcgac ggcggtgtat ctcctcctgc
      901 cctcgttcct gacgcggtac gggcagttgg agccgctgtc gccgcacgac cccgaccagg
      961 ccccactgct ggcacggctg cactcgctgt cccaggaggc gggcctgtca cggccccccg
     1021 agttcctggt gagcggcctg accgaccgca gggtggacgc gtacaccttc ggccgaagcg
     1081 gccgtcaccg ggtggctctg gcccagggaa cagtccgggc cttcggcctc gcgccggagc
     1141 gggcccgtgc cgtcgtcctg cacgaactgg cgcacctgcg gaacaaggac gtcgacgtct
     1201 tctacctcac catcgccctg gccctggcct tcgtccccac gggactgttg ccactggccg
     1261 tcgcactgat cggaacgccg gcctccgggg tcctgtcggt gacctggcgg tctctcgccc
     1321 tggtcgccct cgtctggatc acctgtgtgt ccgtgctgcg gatccgcgag tacggcgccg
     1381 acgtgcgggc ggcctcctgg ggcgcgggac cgggcctgct ggacgtggtc ggcaccgagt
     1441 ccggccgggc cgtgccctgg tggcgtcgtg ggccgctggc cctgcacccg tccgcggccg
     1501 cgcgggcggc cgagatcgtc cggccgcggg agctgttccg tgtccgcttc accgagtgct
     1561 tcgccgcagg gctggccgtc tcggtcgccg cgagcgggct gctcactctg ctgtggctgg
     1621 tggtgaaccg gctcgacgcc ctcgacgcgc gctggacgac cgtgctgctg tgcgcacccg
     1681 ccgccctcgc cgtaatgggc ctcggggtct ggcgcgcggc cctgttcggc ggtggcaccc
     1741 gggcggttct gctgccgggg ctgggcctcg gtctcggtct ggtcgtcggc gggccgctcg
     1801 ccccccagaa cggcatcgtc ctgacgggcc gccccctcgc gccgggaccg gcggcactcc
     1861 tgtcgtccct cgccctgtgc tcgctcatgg tggcgctgtc ggcctggatc gcccggtcgg
     1921 cggcgctctg gcacgccacc ggggagcagc cgccgggaaa ggcgtggcag gccgggctga
     1981 gcgccaccgc gctcccgttc gcggtgctcc tgagcgggtg gatgctgctg tacgacaccg
     2041 gcgacgggct ggggccggtc cacgactcgg cccgggatct gtacggcgtg gtgagcgcct
     2101 cggcaccggt gggcccgttc tggctgtggg ccctcgtgga acacccgttg acgctgttgt
     2161 tcacccggtg gacgccgctc gtcgtggccc tcgtggccct gtggctgttc ccgttggcca
     2221 cgctgtggca ccgtcggccg ccgtacgaca ccgcatgggt ggggcaggtc ctgacgacga
     2281 gcgccgggac tgccggtgtt ttcgcctggt tgcagatcct ggagcgggtg gtgctgcggc
     2341 agggggtgag ccaggcggtg cgcagccagg acggattcgt cctcgcgttc gcctactggc
     2401 agatcgccgc cgcgatgctg ttccagggag tcgccgccgc cgtgacggtc cgctcgcacc
     2461 gccacgaccg gctcgtgata ccgctcgcgc tgctgaccgc ctttctgacc ggttgtctgc
     2521 tgaccctggt gttcttcgcc ggggtcgtcc tggccggctg cgccgacggc ctcgccctgg
     2581 ttccgggtcc gtgctcgctc gaccttccgc tccctctggt gcgcgacaca ctgctgcgga
     2641 tcctggccgg aggtttcctc tgcgcgctgg tgggcgtggc ggtgcccgtc accgtgggac
     2701 gcctgcgcgg cgagcgtccg cccgtggcgc ccggaacgcc cgtgcggcac gccctccgcg
     2761 cacggccgaa ccggtggctc gcggtcggcg tcgccgctct cccggcgtcc ctcgtcctgg
     2821 ggttcgcgct cctcgtcggc cccatcgggc agccgtccga cgtccgcccc gccgactcgt
     2881 cctcggcggc cgcctgccgg gccttcgacg gtctcctcgg gtccatgggt tccctgggcc
     2941 cagccgatgc caacgcccgg ctccacgaag cggtgcggct cgcgctgctg gccggcgaca
     3001 cttccctcgc caccgacttc cagggactgt tcgccgccgc cggggaccag gacgccgaga
     3061 ggttcggcgc cctgaccgac cgcatcggcg accggtgcgc cgacgagggg gctccgctgg
     3121 ggaatccgcc ccggtgaagg cccaccgccc gagccgcggt gaatcgtgtc cgcctgagcg
     3181 ggccgtcctc agccggctca aagcccgttg aacaactccg ccgcaccgca cgtacttccc
     3241 ggtactggcc aagcaattgc cccaacgacc agttggcatc acccgggagg atcactttgc
     3301 ggcgtctcaa cggctcgctc atcgccagcc tggctctcgt cgtcaccgtc ggcgcgctgg
     3361 cgtttcccgt atggtcctac gccgaccgat cgggcaccgc acaggccaac atggccgcca
     3421 gcacggtgaa cacccagtgg gggccgctga cggccgccga ccgggacctc atcatccgcg
     3481 tgcgcctcgc gggactgtgg gagctgccgg ccgccgagaa ggcgatggca cggtcgagca
     3541 gcccggaggt gaaggaggcc gcggaccacc tgatcgtggg tcacaaggac ctcgacgagc
     3601 gggtgcggaa cgtcgcctcg cagctgggcg tcgagctgcc gaacgtgccg aacgagcagc
     3661 aggtgggctt cctcgcgcag atggacaacg cgaccgacga ccagttcgac cgggtctggg
     3721 ccaacctgct gcgctccgcg cacggcaaga tcttcccggc catcggggcg atccggaacc
     3781 agaccgagaa caccctggtg cgccagctgg cctcggacac caaccagacc gtgctcgacc
     3841 acatcacgat gctggagaag accaaccagg tcgacttcga cgcgatcgcc aacggcacga
     3901 tctgacgccg ccccggcccc gcccccgggc ggcgacccgg gaccggacag tcacagcggc
     3961 acccccgcgg tcaccctccg cgccccaagc aacagttcag tccccaacga tcagttggtt
     4021 tcctccggga ggctcatttg cgacgctcca agggctctgt gctcgtcgcc ctggcgatcg
     4081 ccggcacact caccgcggtc gcctacccca tcttctactc gtaccccaac cgcaacgcga
     4141 cagccgcggc cgcgctcacc ggtgacacgg tgaccacgca gtgggggccg ctcacccccg
     4201 ccgaccgtga cctgctgatc cgggtgcggc tggcgggcct gtgggagctg cccgccgggc
     4261 agcaggcgct ggagcgcagc ggcagccagt ccgtcaggga ggccgccgac cacctgatcg
     4321 tgggccacac cgacctcgac aagcggtcac gggtcatcgc cgcgaagctg ggcgtcgagc
     4381 tgccgaacca gccgacggcg gagcagcagg gctggctgga ccagatgacc gcctccaaga
     4441 ccgaggccga gtacaacaag acgtgggcga acctgctgcg ggccgcccac ggcaagatct
     4501 tcccggcgat cgccagcgtg cggaactcca cccgcaacac gctgatccgc cagctggcct
     4561 ccgacgccaa ccagaccgtg ctggaccaca tcacgatcct ggagaagacc ggcgaggtcg
     4621 acttcgcgac gatcgccgac aactcggtga gcggtaccgc cagcccgacc ggcccgccgc
     4681 cgcccgtccc cggccaggtg ccgcccgccg cgcccccggc cgtgccgtcc accaaccctg
     4741 acgtgcagtc gaagccgtcc cccttcgagt ccgggaacgt ccccaccggg cgtccggacc
     4801 cggaggagat caacaaggac cagggtcagc cgcaggtcca gtagaaagct caaatgttgc
     4861 tctgaaggtc ccctccacgg ggttcggccc cggctgtcgg ggccatgact ccgtcacgcc
     4921 ggaacacagg tccgccgtaa ggagacacac agggtcctgg tgaagccgtg accgacgtga
     4981 ccgaggaggg gacggggacg atggagcaga tcgcacagtt ggggacgggg atcggctggc
     5041 ggccggagat cgccgacgcc gtggagcgca tggccggtgc ggacggcggt gtcgactggg
     5101 tcgaggtcgt cgccgagaac gtctgtcccg gccacatcgc cgagtcgttg ctgcgcctgc
     5161 gcgagcgggg cgtcacggtc gtgccgcacg gtgtctccct cggtctcggc ggcgccgacc
     5221 gccccgacga gcagcgcctg cgctcccttg ccgagcgcgc ggaggcgttg ggcgcccccc
     5281 tcgtcaccga gcacatcgcc ttcgtccggg cgggcggcgc cctcaccgcg tcgccgttgc
     5341 tggaggccgg tcacctgctg ccggtgccgc gcacccggga cgccctggac gtgctgtgcg
     5401 agaacgtccg gatcgcccag gacgcgctgc cggtgccgct cgcgctggag aacatcgcgg
     5461 cgctcatcaa ctggccgggc gaggagatga ccgaggggca gttcctctac gacctcgtcg
     5521 accgcaccgg cgtccggctc ctcatcgacg tggcgaacct gcacaccaat catgtcaacc
     5581 ggggcgagga ccccgcgaag gcgctcgacg agctgccggt cgaggccatc gcgtacgtcc
     5641 atgtcgcggg cggcttcgag cgcgacgggg tctggcacga cagccacgcc caccccgtcc
     5701 cgcagcaggt cctcgacgtc ctcgccgacc tcgcctcccg gatcgccccg ccgggcgtgc
     5761 tcctggagcg cgacgacaac ttccccgagc cgggggagct ggagcgcgag ctgacggcga
     5821 tccggggtgt gctggagaag gtggaggtgc ggggccccgg caaggatgta cgggagcccg
     5881 agaagggtgc gcgcgagacc gacaggggtg agcgggtccc cgggcggaag gcggccacgg
     5941 cccgggcgaa ggccgtcgcg gagaccgagc ccgccgccga ggccgtaccc gtcaccgagg
     6001 ccgagcccgc agcccggcag cggctcgggc tcgcgcaggc ggcgctgctg tccgcgctgg
     6061 tcgcggggac cccggtgccc gaggggttcg accgggtgcg gctgggcgtc caggcgcggg
     6121 ccctcgcggc caagcgggcg gacgtggtgg ccaaggtcgc gccggagctc ccggagatcc
     6181 tcggatcgtc gtaccggccg acctttgtcg cgtacgcaca ggggcacccg atgaccgacg
     6241 gttaccggcg ggacgccctg tccttcgccg agcacgtgct gctcacgggc cgcccggagg
     6301 acgcggacgt ccggcgcgag gtgcgccgct ggtggctgga gcgatcgggc cccgcgccgc
     6361 tctcccagcg gccgacggcc cggctggccc gcgcgacccg cagggtcctg ctacgccggt
     6421 gagccggggc gttcaccccc ggatgtgctg cgattaacag acccgtgcct cccgcacggg
     6481 tttgccgccc tgtgcgccat gttttgtcgc ggttcatccg gcccttcgcg taccccatcc
     6541 ggcgcgcttt gtcccctcct caaccgttcc tcctcatacg gcagttggcg tagaccgggc
     6601 agtaacatgc cagtgtgtag gccgatcagc acccgttgcg cactagcgtg cggtgccgcg
     6661 agcaggaggc accatgcgaa ccaccaacgg cagaggagga ctgttcagtg gcacaggact
     6721 catcgtcgcc ggactgacgg cgacgctcgt cgccttgctc ttcccgatct ggtcgtacgc
     6781 ggaccgctcg ggcaccggcg tgagcgtgct caacgcgtcc accgtgacga cgcagtacgg
     6841 cccgctgtcc gccctggacc gcgacttcgt caccaaggtc agactggcgg ggctgtggga
     6901 gctgccggcc gggcagcagg cccagacgaa gggctccacc ctggccgtac gcaccgcggg
     6961 ggagcatctc gtcgaggggc acaccttcct cgacgcgcgg gtccgcaagg tcgccgcgcg
     7021 cctcgccctg cccctgccca accagccgag cgaccagcag aaggcgtggc tggacaccct
     7081 gacggcggcg cagggcgaga cctacgaccg cgagttcgcc aacatcctgc ggctggcaca
     7141 cggcaaggtc ttctccgtcg tcgcccaggt ccgggccacc acccgcaact cgctggtgcg
     7201 tgacctcgcc gacgacgcca acaccaccgt tctcgaccac atcaaggtgc tggaggccac
     7261 cgggttcgtc gactacgacg cgatcgcccg cgacgccgcc tccgcgagcg ccccgccgct
     7321 caccaactcc cccacgccgc cgaccccgtt ggacgacccc ggctcccccg tcccggtgac
     7381 cccctcgccc acgccgaccc ggccggaccc gtcaccgacg tacgccctgc ctccggccgc
     7441 caacggcccg gcggacgacg actgaggacg gacgacaggg caccggccgg acccgaaggc
     7501 tccggccgcc cgcccctgcc gggtcctggc aaatgcgggc cgcgacggcg aacaatcgga
     7561 acagagcggc caccttccct gcggattacg gacatcgcgc gatgcggccc ggggcaggcg
     7621 catagaaaca cgccatgatc tgggtcctct tcctcctgct ggcctgggcc gccgccggcg
     7681 tgtcctgtgc ccgactctgt ctgacctcga tgcgtgcggc ggcggccgac tccgacgtcc
     7741 acgtcgacca cgcccaccac tgccaccggc tcacgctgta cgaggcggcg ttcctgtccg
     7801 gcggcccggc acgcgtcgcc gacctcaccc tggtctccat ggccggccgg cgcggactcc
     7861 tgctcgcgca caccggttgg gtgacggtgg tggacccgga cggccgggac gacatggaac
     7921 gctccgtgat cggcgcgata ggccccggcg ggcagtcccg gatagcgccc gtacgccgga
     7981 ccgccgcagc cgcggacccc gtacgccgcc tctccgaccg gctcgtcgcg gcggggctgg
     8041 ccgtgcccta cgccgcgcgc acggccgtgg ccgacgcggt cacccaggta cggggcgccg
     8101 cgctgggcgt gtgcggactg ggggcgctgg cgttgatgct gccctcgcag gggtacgggg
     8161 agcatcacgt gctggtcgcg ctgtggttcg cgttgccgct ggtgctgacg ctcagctgtc
     8221 tggtcatcgt gcgggtcgac aggcatcccg gtacgcgctg ggcgacaccg gagggggtgc
     8281 gggtgctgcg gaccgtcgtg ggccggggcg gaggtgacct ggcggatctc gtctccgtgg
     8341 ccgtctgggg cgtgggggcg gtggggtccg cggagctgcg ggcggcgttc gggcagcgat
     8401 agcgacggcg acactccccg acagtccttt acattcgccc tatttaacgc caaacatccc
     8461 tttgtcgctg tcgtgcttcg gagggattcg ccatgcgggc tgccgttctc tacgggactg
     8521 tcggggcggt ggtgctgagt ggcctcgggg ccgcgccggc cgagggggac atgagccgga
     8581 cggcggcgca gtcggtcgcc ctcgcggccg agcgggccgc ggacgagggc atccggttcg
     8641 gtaagtgccc ggcggcggag gggctgccgg acggggtgcg gtgcgggacg gtcgaggtgc
     8701 cgctcgacta cgcacggccc gacggccggc agatctcact gaccgtcagc cggatcgcgg
     8761 ccaagggcaa ggacgcgaag ggcaggaagg tcgcccggca gggggcgctc gtgttcaacc
     8821 cgggcgggcc cggcgcctcc gggatgtatt tcccgctcgc cggctacctc ccgggctgga
     8881 agcgcatcgg cgccgcctac gacctagtcg gctacgcccc gcgcggggtc ggccgctcgg
     8941 caccgctgtc ctgccaggac ccgaagcgac tgcaccgggg gcccgcaccg gcgccgacgc
     9001 acccctcgga gacgtacaag aaggaacgcg tcgcacgggc caaggcgtac gcgcgaggct
     9061 gtgcacggcg gggcgggagc gcgctgcggc actaccactc cctcaacaac gcccgtgacc
     9121 tggacgtgct gcgcgccgcg ctcggcgagc cacgactgac gttcatgggg gcgtcgtacg
     9181 ggacgtacat cggggcgctg tacgcggccc tgttcccctc gcacgtacgc cggatggtct
     9241 tcgactcggc cgtgaacccg gcccccgagc agatctggta ccgcaacaac ctcgaccagt
     9301 cggccgcgtt cgaggggcgc tgggcggact tccgggcctg ggtcgccagg caccaccggg
     9361 tctacgggct gggtgacacc ccgcagaagg tgctgcggag ctacgagcgg gcggcggcgc
     9421 ggctggccgc ccggcccgcc gccggcaagg tgggcccggg ccagctgcag ggcgcgttcc
     9481 tccaggccgc gtaccacgac gaccactggc cgcagcgtgc cctggcgctc tcggcgtatc
     9541 tgaggggcga cccgaagccg ctcgtccgga tcgccggccc gtacccggaa gccgccgtcg
     9601 aacaggagaa cgcgaaggcc gtctacacgg ccgtcgagtg caacgacgcg ccctggccga
     9661 cacggtggag cgtctgggac cgcgacaaca cccggctcgc gcggaccgcg cccttcgaga
     9721 cgtgggacaa cgtgtggacg aacctgccgt gcgcctactg gagcggtccg cgtcaacgac
     9781 cgctcgacgt gcggaccggc cccggcgagc tgccgccgac gctgatcctg gcggccgagc
     9841 gggacgcggc cgcgccgtac gacggagcga tcgagctgca ccggcggctg tggggttcgg
     9901 tactggtgac cgagcgggac gcggggaacc acggggtcgc gggcgggtcc aacacctgcg
     9961 tcaacgggta cctggaggcg tacctgctgc aggggcgggc gcccgtgcgg agggccgcgt
    10021 gcgcggggcg gccggagccg agcgccgctc ggcgcgaggc tgtcggggaa ctgcgggtcg
    10081 tcggtagctg atcgcgcagt tccccgcgcc ccttacgggg cctaggcgag gcccgccacc
    10141 agctccgcca ccgacttgcg gcggcccgtg tagaacggga cctcctcgcg gacgtgcatc
    10201 cgggcctccg aggcacgcag gtgccgcatg aggtcgacga tgcggtacag ctcgtcggcc
    10261 tcgaaggcga ggatccactc gtagtcgccc agtgagaagg aggcgaccgt gttggcccgg
    10321 acgtcggggt agccgcgggc catcttgccg tggtcggcga gcatgcggcg gcggtcctcg
    10381 tcgggcagca ggtaccagtc gtaggagcgc acgaaggggt agacgctgac gtagtcgcgg
    10441 ggcgtctcgt cggcgaggaa cgccgggatg tgcgaccggt tgaactcggc ggggcggtgc
    10501 agcgccatgt tcgaccacac cggggcgagc gcgcgaccca gccgggtgcg gcggaagagg
    10561 ttgtacgcct cctggagctg gtcgctggtc tcggcgtgcc accagatcat gacgtcggcg
    10621 tcggcgcgca ggcccgacac gtcgtacgta ccgcggatgg tcacgtcctt ggcggcgagc
    10681 tggtcgaaca gctcctggac ctcgtcggcg taaccggcgc ggtcctccgg cagcacgtcc
    10741 ttcagcctga agacggacca cagcgtgtaa cggatgactt cgttgaggtc cttggccagc
    10801 ttgcccttgt tcgggatccg gccggactcg gtggtggggg cgtcgtcgct catgggtctc
    10861 attctcccgc tccgccgtgg aggctctgca ccgggtgggc ggtgaggcgc cgcaccgcgc
    10921 gcaggtcacc gtggatctgg tcgaccgcgg cagcggcgct cgcgacgcac gccgggatgc
    10981 cgacgccgtc gtacgccgcg ccgcagaccg cgaggcccgg cagcttgccg aggtgctcgc
    11041 gcaggcgcgc cacgcgcgcg tggtggccga ccgggtactg cggcaggccg tcgtcccagc
    11101 gggtgacgcg ggtctccagg gggacggcgg acagaccggt ggcctcgtgc aggtcggttc
    11161 tcgacacctc caccaggtgg gcgtcgtcgc gctccaggat cgccgtctcg ccgtaccggc
    11221 cgaccgaggt gcgcaggacc agcaggtccg ggttctcgtc ggcgatccag ccccacttcc
    11281 gggaggcgaa ggtggacgcc ttgatggtgc ggccgtcgac cggcggcacc aggaagccgc
    11341 tgccctcggg gagggtgacg tcggcgcggc ggtaggcgag ggtgaccagg gccatcgagg
    11401 cgtactcgac agcggccagc tc
//
