GCF_019455805.1 - 11 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_SISU01000010.1 (Clavibacter michiganensis subsp. michiganensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 3.1	NRPS-like	1	32,370

NZ_SISU01000001.1 (Clavibacter michiganensis subsp. michiganensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 8.1	hydrogen-cyanide	565,162	578,444
Region 8.2	lipolanthine,lanthipeptide-class-iii	696,607	726,202
Region 8.3	NAPAA	762,783	796,952	High	ε-Poly-L-lysine	NRPS:Type I
Region 8.4	NRP-metallophore,NRPS	1,070,621	1,127,074	Medium	coelibactin	NRPS:Type I

NZ_SISU01000002.1 (Clavibacter michiganensis subsp. michiganensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 9.1	T3PKS	271,547	312,767
Region 9.2	terpene-precursor,betalactone	658,245	693,910
Region 9.3	lanthipeptide-class-ii	768,083	791,244	High	michiganin A	ribosomal:RiPP:Lanthipeptide

NZ_SISU01000003.1 (Clavibacter michiganensis subsp. michiganensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 10.1	terpene	114,994	139,415	Low	carotenoid	terpene
Region 10.2	azole-containing-RiPP	221,907	244,196

NZ_SISU01000005.1 (Clavibacter michiganensis subsp. michiganensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 12.1	NI-siderophore	40,830	71,334	Low	legonoxamine A/desferrioxamine B/legonoxamine B	other:other

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 3.1	NRPS-like	1	32,370
Region 8.1	hydrogen-cyanide	565,162	578,444
Region 8.2	lipolanthine,lanthipeptide-class-iii	696,607	726,202
Region 8.3	NAPAA	762,783	796,952	High	ε-Poly-L-lysine	NRPS:Type I
Region 8.4	NRP-metallophore,NRPS	1,070,621	1,127,074	Medium	coelibactin	NRPS:Type I
Region 9.1	T3PKS	271,547	312,767
Region 9.2	terpene-precursor,betalactone	658,245	693,910
Region 9.3	lanthipeptide-class-ii	768,083	791,244	High	michiganin A	ribosomal:RiPP:Lanthipeptide
Region 10.1	terpene	114,994	139,415	Low	carotenoid	terpene
Region 10.2	azole-containing-RiPP	221,907	244,196
Region 12.1	NI-siderophore	40,830	71,334	Low	legonoxamine A/desferrioxamine B/legonoxamine B	other:other

No secondary metabolite regions were found in these records:: NZ_SISU01000006.1 (Clavibacter michiganensis subsp. michiganensis); NZ_SISU01000009.1 (Clavibacter michiganensis subsp. michiganensis); NZ_SISU01000011.1 (Clavibacter michiganensis subsp. michiganensis); NZ_SISU01000012.1 (Clavibacter michiganensis subsp. michiganensis); NZ_SISU01000013.1 (Clavibacter michiganensis subsp. michiganensis); NZ_SISU01000014.1 (Clavibacter michiganensis subsp. michiganensis); NZ_SISU01000004.1 (Clavibacter michiganensis subsp. michiganensis); NZ_SISU01000007.1 (Clavibacter michiganensis subsp. michiganensis); NZ_SISU01000008.1 (Clavibacter michiganensis subsp. michiganensis)

Compact view

NZ_SISU01000010.1 - Region 1 - NRPS-like

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Location: 1 - 32,370 nt. (total: 32,370 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: PPTase carboxylase

EOG37_RS15725: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

EOG37_RS15750: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

Hydrolases: hydrolase

EOG37_RS15690: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: dehydratase

EOG37_RS15745: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Translocases: permease

EOG37_RS15740: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000893.5	0.44	NRPS	chloramphenicol	Streptomyces venezuelae ATCC 10712
BGC0001873.3	0.44	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0001948.3	0.42	other (other)	naseseazine C, C3-aryl pyrroloindolines	Streptomyces sp.
BGC0000234.5	0.42	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002387.2	0.41	PKS	yangpumicin A	Micromonospora yangpuensis
BGC0000275.4	0.41	PKS	tetracenomycin C	Streptomyces glaucescens
BGC0002284.3	0.40	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0001468.5	0.40	other (trna-derived)	bicyclomycin	Streptomyces cinnamoneus
BGC0002591.2	0.40	PKS, terpene	aurachin C, aurachin D, aurachin SS	Streptomyces sp.
BGC0001627.4	0.39	NRPS	isonitrile lipopeptides	Mycobacterium tuberculosis H37Rv

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000893.5	0.44	NRPS	chloramphenicol	Streptomyces venezuelae ATCC 10712
BGC0001873.3	0.44	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0001948.3	0.42	other (other)	naseseazine C, C3-aryl pyrroloindolines	Streptomyces sp.
BGC0000234.5	0.42	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002387.2	0.41	PKS	yangpumicin A	Micromonospora yangpuensis
BGC0000275.4	0.41	PKS	tetracenomycin C	Streptomyces glaucescens
BGC0002284.3	0.40	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0001468.5	0.40	other (trna-derived)	bicyclomycin	Streptomyces cinnamoneus
BGC0002591.2	0.40	PKS, terpene	aurachin C, aurachin D, aurachin SS	Streptomyces sp.
BGC0001627.4	0.39	NRPS	isonitrile lipopeptides	Mycobacterium tuberculosis H37Rv

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (9):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.52 out of 21.55

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.99 out of 30.97

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.14 out of 32.93

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 25.75

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.17 out of 35.63

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.98 out of 30.11

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.60 out of 30.11

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.00 out of 20.01

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 32370:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

EOG37_RS15745: X

AMP-binding (24..465): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RHALHLTGRSSAVFICTSAHDLYGSTEAGGILHD
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description

NZ_SISU01000001.1 - Region 1 - hydrogen-cyanide

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Location: 565,162 - 578,444 nt. (total: 13,283 nt) Show pHMM detection rules used

hydrogen-cyanide: (Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

EOG37_RS02660: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

EOG37_RS02685: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

Hydrolases: peptidase

EOG37_RS02695: methionine aminopeptidase

smcogs: SMCOG1172: methionine aminopeptidase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002345.2	0.53	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000504.5	0.40	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000538.3	0.39	ribosomal	nisin Z	Lactococcus lactis
BGC0002382.3	0.39	ribosomal	grimoviridin	Grimontia marina
BGC0001289.5	0.39	ribosomal	penisin	Paenibacillus ehimensis
BGC0000500.5	0.39	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0002064.2	0.37	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10
BGC0000497.3	0.37	ribosomal	BHT-Aα, BHT-Aβ	Streptococcus ratti
BGC0000541.5	0.37	ribosomal	paenicidin A	Paenibacillus polymyxa

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002345.2	0.53	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000504.5	0.40	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000538.3	0.39	ribosomal	nisin Z	Lactococcus lactis
BGC0002382.3	0.39	ribosomal	grimoviridin	Grimontia marina
BGC0001289.5	0.39	ribosomal	penisin	Paenibacillus ehimensis
BGC0000500.5	0.39	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0002064.2	0.37	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10
BGC0000497.3	0.37	ribosomal	BHT-Aα, BHT-Aβ	Streptococcus ratti
BGC0000541.5	0.37	ribosomal	paenicidin A	Paenibacillus polymyxa

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.35 out of 20.47

Confidence: strong

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.57 out of 26.33

Confidence: medium

TFBS Finder

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_SISU01000001.1 - Region 2 - lanthipeptide-class-iii,lipolanthine

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Location: 696,607 - 726,202 nt. (total: 29,596 nt) Show pHMM detection rules used

lipolanthine: (micKC and Flavoprotein)
lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidase

EOG37_RS03290: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

EOG37_RS03315: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

Transferases: acetyltransferase

EOG37_RS03330: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: hydrolase

EOG37_RS03250: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: ligase synthase

EOG37_RS03320: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

EOG37_RS03325: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000513.3	0.32	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000551.5	0.31	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0002711.2	0.30	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0001152.5	0.27	NRPS, PKS	fusaricidin B	Paenibacillus polymyxa
BGC0000520.5	0.26	ribosomal	lacticin 3147 A1, lacticin 3147 A2	Lactococcus lactis
BGC0000255.5	0.26	PKS	pederin	Uncultured bacterium
BGC0000516.4	0.25	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0000626.3	0.24	ribosomal	thuricin	Bacillus thuringiensis
BGC0000546.3	0.24	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0002591.2	0.24	PKS, terpene	aurachin C, aurachin D, aurachin SS	Streptomyces sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002711.2	0.47	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0000513.3	0.46	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000551.5	0.46	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0001152.5	0.45	NRPS, PKS	fusaricidin B	Paenibacillus polymyxa
BGC0000888.5	0.44	other (other)	bacilysin	Bacillus sp. CS93
BGC0000255.5	0.44	PKS	pederin	Uncultured bacterium
BGC0000231.5	0.43	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001895.4	0.43	PKS	huanglongmycin A, huanglongmycin B, huanglongmycin C	Streptomyces sp. CB09001
BGC0001869.4	0.42	NRPS, other (phenazine)	griseoluteic acid, demethoxygriseoluteic acid, phenaszentine A, phenaszentine C, phenaszenketide C, phenaszenketide D	Xenorhabdus szentirmaii DSM 16338
BGC0000516.4	0.42	ribosomal	geobacillin II	Geobacillus thermodenitrificans

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.36 out of 21.84

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.53 out of 21.84

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.80 out of 28.19

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description

NZ_SISU01000001.1 - Region 3 - NAPAA

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Location: 762,783 - 796,952 nt. (total: 34,170 nt) Show pHMM detection rules used

NAPAA: (AMP-binding and TIGR02353)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EOG37_RS03665: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Transferases: PPTase aminotransferase

EOG37_RS03610: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

EOG37_RS03655: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Ligases: ligase

EOG37_RS03645: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.77	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001135.5	0.61	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001833.4	0.60	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001240.4	0.59	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.77	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001135.5	0.61	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001833.4	0.60	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001240.4	0.59	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (4):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.32 out of 29.25

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.07 out of 33.73

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.02 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.97 out of 24.78

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 762782 - 796952:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

EOG37_RS03645: X

AMP-binding (56..456): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAELFDASCEELSTIFGGEWNTYGPTEATVVACA
Nearest Stachelhaus code(s):: Lys DAEDIGTVVK (71% 8Å match); Gln or Tyr DAESVGTVVK (65% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description

NZ_SISU01000001.1 - Region 4 - NRP-metallophore,NRPS

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Location: 1,070,621 - 1,127,074 nt. (total: 56,454 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase reductase

EOG37_RS05295: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

EOG37_RS05325: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

Transferases: PPTase kinase

EOG37_RS05145: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

EOG37_RS05235: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

Hydrolases: esterase

EOG37_RS05265: thioesterase

smcogs: SMCOG1004: thioesterase

Isomerases: isomerase

EOG37_RS05230: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase

EOG37_RS05240: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

EOG37_RS05245: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

EOG37_RS05250: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Translocases: transporter

EOG37_RS05140: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000412.5	0.63	NRPS	pyochelin	Pseudomonas aeruginosa PAO1
BGC0001833.4	0.61	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.60	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.60	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.60	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.58	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.58	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.58	NRPS	AbT1	Aureobasidium pullulans
BGC0001166.5	0.57	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000412.5	0.65	NRPS	pyochelin	Pseudomonas aeruginosa PAO1
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.63	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.62	NRPS	AbT1	Aureobasidium pullulans
BGC0001166.5	0.62	NRPS	HC-toxin	Alternaria jesenskae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.79 out of 25.31

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.99 out of 20.47

Confidence: medium

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 26.92 out of 32.74

Confidence: medium

Additional weak hits (18):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.86 out of 30.22

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.28 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.29 out of 27.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.68 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.20 out of 26.06

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.16 out of 32.74

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.54 out of 21.55

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.30 out of 20.01

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.65 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.67 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.18 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.32 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.56 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.09 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.25 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.03 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.17 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.39 out of 29.65

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 4, location 1070620 - 1127074:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser - Cys) + (Me-Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

EOG37_RS05240: X

AMP-binding (44..464): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: NAALFTLACPGGALQVGGAQQVYGMAEGLVCMTG
Nearest Stachelhaus code(s):: Sal TLPAQGVICK (79% 8Å match); n-oh-nitropheBut TLPSQGVVCK (74% 8Å match)
Stachelhaus code match:: 90% (moderate)

EOG37_RS05245: Ser - Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (588..991): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TSLSFDLSVYDANTLLGGDLALGGMTEATIHSTV
Nearest Stachelhaus code(s):: Ser DLYNLGLIHK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1659..2058): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TAVSFDLSVFDGDTLVSGDVALGGATEAAIWSNA
Nearest Stachelhaus code(s):: Cys DLFNLSLIWK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

EOG37_RS05250: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (543..955): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDIAFDLSVFDGNSLLSGDVSLGGATEAAIWSVH
Nearest Stachelhaus code(s):: Cys DLFNLSLIWK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
FuR variant 2	Ferric Uptake Regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_SISU01000002.1 - Region 1 - T3PKS

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Location: 271,547 - 312,767 nt. (total: 41,221 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

EOG37_RS07610: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

EOG37_RS07660: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acetyltransferase

EOG37_RS07675: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: hydrolase

EOG37_RS07560: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Isomerases: isomerase

EOG37_RS07710: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: synthase

EOG37_RS07650: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.53	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001962.3	0.51	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0001905.4	0.50	PKS	violapyrone B	Streptomyces somaliensis
BGC0002332.2	0.50	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.
BGC0001077.4	0.49	PKS	6-anteisoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-geranyl-2-methoxyhydroquinone, 6-palmityl-4-O-geranyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-palmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone	Actinoplanes missouriensis 431
BGC0001831.3	0.46	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0001529.5	0.45	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0002362.3	0.43	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0002151.2	0.42	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0002150.2	0.42	ribosomal	triculamin	Streptomyces triculaminicus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.53	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001962.3	0.51	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0001905.4	0.50	PKS	violapyrone B	Streptomyces somaliensis
BGC0002332.2	0.50	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.
BGC0001077.4	0.49	PKS	6-anteisoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-geranyl-2-methoxyhydroquinone, 6-palmityl-4-O-geranyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-palmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone	Actinoplanes missouriensis 431
BGC0001831.3	0.46	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0001529.5	0.45	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0002362.3	0.43	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0002151.2	0.42	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0002150.2	0.42	ribosomal	triculamin	Streptomyces triculaminicus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 26.01 out of 29.65

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.65 out of 24.78

Confidence: medium

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 24.83 out of 29.65

Confidence: medium

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 26.01 out of 29.65

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.65 out of 24.78

Confidence: medium

Additional weak hits (5):

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.03 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.21 out of 24.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.31 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.56 out of 28.14

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.53 out of 21.84

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
LexA variant 1	Repressor of DNA damage response
LexA variant 2	Repressor of DNA damage response

NZ_SISU01000002.1 - Region 2 - betalactone,terpene-precursor

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Location: 658,245 - 693,910 nt. (total: 35,666 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)
betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

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resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

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KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

EOG37_RS09440: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Transferases: acyltransferase carboxylase

EOG37_RS09420: acyltransferase

smcogs: SMCOG1141: acyltransferase

EOG37_RS09445: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

Hydrolases: deformylase

EOG37_RS09435: peptide deformylase

smcogs: SMCOG1275: peptide deformylase

Ligases: ligase synthetase

EOG37_RS09395: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

EOG37_RS09430: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.31	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002309.2	0.29	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001911.3	0.26	terpene	raimonol	Streptomyces anulatus
BGC0001910.4	0.26	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000664.5	0.26	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.26	terpene	isorenieratene	Streptomyces argillaceus
BGC0002561.3	0.25	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000633.3	0.25	terpene	carotenoid	Streptomyces avermitilis
BGC0000936.5	0.25	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0000649.3	0.25	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002309.2	0.47	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000677.5	0.46	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001911.3	0.43	terpene	raimonol	Streptomyces anulatus
BGC0000649.3	0.43	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002284.3	0.43	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0001910.4	0.43	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000664.5	0.43	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.42	terpene	isorenieratene	Streptomyces argillaceus
BGC0002561.3	0.42	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000678.5	0.42	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

EOG37_RS09395: PT_FPPS_like

Domain 1 (45 - 371): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.21 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 15.77 out of 33.14

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description

NZ_SISU01000002.1 - Region 3 - lanthipeptide-class-ii

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Location: 768,083 - 791,244 nt. (total: 23,162 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

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Gene overview

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Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EOG37_RS09920: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

Lyases: cyclase

EOG37_RS09895: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Ligases: synthase

EOG37_RS09940: Pseudouridine synthase

smcogs: SMCOG1154: Pseudouridine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.99	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001579.4	0.50	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000527.5	0.50	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002346.3	0.49	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000503.5	0.48	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001210.5	0.47	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000539.5	0.47	ribosomal	nukacin ISK-1	Staphylococcus warneri
BGC0000626.3	0.47	ribosomal	thuricin	Bacillus thuringiensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.99	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001579.4	0.50	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000527.5	0.50	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002346.3	0.49	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000503.5	0.48	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001210.5	0.47	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000539.5	0.47	ribosomal	nukacin ISK-1	Staphylococcus warneri
BGC0000626.3	0.47	ribosomal	thuricin	Bacillus thuringiensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for EOG37_RS09895

EOG37_RS09890

Leader:

MNDILETETPVMVSPRWDMLLDAGEDTSPSVQTQIDAEFRRVVSPYM

Core:

SSSGWLCTLTIECGTIICACR

Core with dehydrations:

DhaDhaDhaGWLCysDhbLDhbIECysGDhbIICysACysR

antiSMASH-DB 4.0 matches for EOG37_RS09890

100.0%

NC_009480.1and 10 others:

NZ_CP033724.1 Lanthipeptides: EFE39_RS10220_lanthipeptideNZ_CP047051.1 Lanthipeptides: GRD74_RS10365_lanthipeptideNZ_CP047054.1 Lanthipeptides: GRD61_RS10460_lanthipeptideNZ_CP053860.1 Lanthipeptides: DQP62_RS10825_lanthipeptideNZ_CP076352.1 Lanthipeptides: KN218_RS09900_lanthipeptideNZ_CP085137.1 Lanthipeptides: LHJ48_RS10290_lanthipeptideNZ_CP085140.1 Lanthipeptides: LHJ47_RS10305_lanthipeptideNZ_CP095274.1 Lanthipeptides: MU580_RS10460_lanthipeptideNZ_PSTW01000024.1 Lanthipeptides: C5C03_RS09675_lanthipeptideNZ_SISU01000002.1 Lanthipeptides: EOG37_RS09890_lanthipeptide

Lanthipeptides: CMM_RS10575_lanthipeptide

1

SSSGWLCTLTIECGTIICACR

21

SSSGWLCTLTIECGTIICACR

1

SSSGWLCTLTIECGTIICACR

21

90.5%

NZ_CP028130.1:

Lanthipeptides: C7V51_RS14690_lanthipeptide

2

SSGWLCTLTIECGTIICACR

21

SSGW+CTLTIECGTIICACR

2

SSGWMCTLTIECGTIICACR

21

90.5%

NZ_CP061274.1and 1 other:

NZ_JADKRR010000001.1 Lanthipeptides: ITJ40_RS10085_lanthipeptide

Lanthipeptides: H9X71_RS09850_lanthipeptide

1

SSSGWLCTLTIECGTIICACR

21

+SSGWLCTLTIECGT ICACR

1

ASSGWLCTLTIECGTFICACR

21

with 109 more sequence matches

Only the first 10 are displayed here.

85.7%

NZ_AUBN01000022.1:

Lanthipeptides: G448_RS16025_lanthipeptide

1

SSSGWLCTLTIECGTIICACR

21

+SSGW CTLTIECGT ICACR

1

ASSGWFCTLTIECGTFICACR

21

81.0%

NZ_CP015515.1and 1 other:

NZ_PSUO01000011.1 Lanthipeptides: C5C18_RS08595_lanthipeptide

Lanthipeptides: A6122_RS12855_lanthipeptide

2

SSGWLCTLTIECGTIICACR

21

SSGW+CTLTIECGT++CACR

2

SSGWMCTLTIECGTVVCACR

21

81.0%

NZ_JAGPXN010000019.1:

Lanthipeptides: KBX06_RS22045_lanthipeptide

2

SSGWLCTLTIECGTIICACR

21

SSGW+CTLTIECGT++CACR

1

SSGWVCTLTIECGTVVCACR

20

76.2%

NZ_CP036455.1:

Lanthipeptides: EKD16_RS25235_lanthipeptide

2

SSGWLCTLTIECGTIICACR

21

SSGW+CTLTIECGT++CAC+

1

SSGWVCTLTIECGTLVCACQ

20

76.2%

NZ_JAODUN010000003.1:

Lanthipeptides: BXY51_RS10560_lanthipeptide

2

SSGWLCTLTIECGTIICAC

20

SSGW+CTLTIECGT++CAC

1

SSGWVCTLTIECGTLVCAC

19

76.2%

NZ_KB895893.1and 1 other:

NZ_KB900614.1 Lanthipeptides: SALPAC6_RS0114155_lanthipeptide

Lanthipeptides: C569_RS0129325_lanthipeptide

2

SSGWLCTLTIECGTIICACR

21

SSGW+CTLTIECGT++CAC+

1

SSGWVCTLTIECGTVVCACQ

20

40.9%

NZ_CP034669.1:

Lanthipeptides: EJ065_RS32040_lanthipeptide

1

SSSGWLCTLTIECG

14

+S G++CTLT ECG

8

NSCGYVCTLTTECG

21

40.9%

NZ_NIHT01000008.1and 1 other:

NZ_NIHT01000008.1 Lanthipeptides: CDL23_RS06740_lanthipeptide

Lanthipeptides: CDL23_RS06745_lanthipeptide

1

SSSGWLCTLTIECGTIICAC

20

+ SGWL T+T +C I C

2

NGSGWLPTITDDCPNSIFVC

21

38.1%

NZ_CP022413.2and 1 other:

NZ_JAQEYW010000009.1 Lanthipeptides: PG422_RS09400_lanthipeptide

Lanthipeptides: CGC63_RS01420_lanthipeptide

3

SGWLCTLTIECGTII

17

SG +CT+++ECG+I

1

SGVVCTISVECGSIF

15

38.1%

NZ_CP036073.1and 11 others:

NZ_CP036073.1 Lanthipeptides: EXW45_RS04930_lanthipeptideNZ_CP036073.1 Lanthipeptides: EXW45_RS04925_lanthipeptideNZ_CP036073.1 Lanthipeptides: EXW45_RS04920_lanthipeptideNZ_CP036073.1 Lanthipeptides: EXW45_RS04915_lanthipeptideNZ_CP036073.1 Lanthipeptides: EXW45_RS04910_lanthipeptideNZ_NUEG01000031.1 Lanthipeptides: CN905_RS18915_lanthipeptideNZ_NUEG01000031.1 Lanthipeptides: CN905_RS18910_lanthipeptideNZ_PVRT01000001.1 Lanthipeptides: C6359_RS05275_lanthipeptideNZ_PVRT01000001.1 Lanthipeptides: C6359_RS05270_lanthipeptideNZ_PVRT01000001.1 Lanthipeptides: C6359_RS05265_lanthipeptideNZ_PVRT01000001.1 Lanthipeptides: C6359_RS05260_lanthipeptide

Lanthipeptides: EXW45_RS04935_lanthipeptide

4

GWLCTLTIECGTIICAC

20

GWLCT+T +C + C

4

GWLCTVTDDCPNSVFVC

20

MIBiG 4.0 matches for EOG37_RS09890

26.7%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

1

SSSGWLCTLTIEC

13

++ GW CTLT EC

14

NNGGW-CTLTKEC

25

25.0%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

2

SSGWLCTLTIEC

13

+ G CTLT+EC

12

NKGAYCTLTVEC

23

12.8%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

7

CTLTIEC

13

CTL+ EC

28

CTLSKEC

34

allorf_782985_783149

Leader:

MTFSDQICANISAVCPLLQRLSLAYM

Core:

FLPCHFQKLGRSTWIQSAARLRPVALAA

Core with dehydrations:

FLPCysHFQKLGRDhaDhbWIQDhaAARLRPVALAA

antiSMASH-DB 4.0 matches for allorf_782985_783149

100.0%

NZ_SISU01000002.1:

Lanthipeptides: allorf_782985_783149_lanthipeptide

1

FLPCHFQKLGRSTWIQSAARLRPVALAA

28

FLPCHFQKLGRSTWIQSAARLRPVALAA

1

FLPCHFQKLGRSTWIQSAARLRPVALAA

28

11.8%

NZ_CAKMMP010000011.1and 5 others:

NZ_CP018620.1 Lanthipeptides: BS614_RS05680_lanthipeptideNZ_CP062165.1 Lanthipeptides: JNUCC31_RS16755_lanthipeptideNZ_FMVM01000006.1 Lanthipeptides: BLQ13_RS10365_lanthipeptideNZ_RIAS01000010.1 Lanthipeptides: EC604_RS18225_lanthipeptideNZ_VEJI01000010.1 Lanthipeptides: FS576_RS22075_lanthipeptide

Lanthipeptides: L6H98_RS19925_lanthipeptide

4

CHFQKLGRSTWI

15

CH+ + S+W+

20

CHYNSVSPSSWL

31

allorf_784456_784641

Leader:

MGQRAVAQLGS

Core:

ALDWGSRGRRFKSCQPDGHASEGPNGPSEAFFVKRAYRQGAQVLSAQIFI

Core with dehydrations:

ALDWGDhaRGRRFKDhaCysQPDGHADhaEGPNGPDhaEAFFVKRAYRQGAQVLDhaAQIFI

antiSMASH-DB 4.0 matches for allorf_784456_784641

100.0%

NZ_SISU01000002.1:

Lanthipeptides: allorf_784456_784641_lanthipeptide

1

ALDWGSRGRRFKSCQPDGHASEGPNGPSEAFFVKRAYRQGAQVLSAQIFI

50

ALDWGSRGRRFKSCQPDGHASEGPNGPSEAFFVKRAYRQGAQVLSAQIFI

1

ALDWGSRGRRFKSCQPDGHASEGPNGPSEAFFVKRAYRQGAQVLSAQIFI

50

58.0%

NZ_CP033724.1and 2 others:

NZ_CP085137.1 Lanthipeptides: allorf_2198282_2198461_lanthipeptideNZ_CP085140.1 Lanthipeptides: allorf_2198287_2198466_lanthipeptide

Lanthipeptides: allorf_2198300_2198479_lanthipeptide

1

ALDWGSRGRRFKSCQPDGHASEGPNGPSEAFFVKRA

36

ALDWGSRGRRFKSCQPDGHASEGP GP+ AF + A

1

ALDWGSRGRRFKSCQPDGHASEGPMGPAGAFVIAVA

36

58.0%

NZ_CP047051.1and 1 other:

NZ_CP076352.1 Lanthipeptides: allorf_2115916_2116095_lanthipeptide

Lanthipeptides: allorf_2221797_2221976_lanthipeptide

1

ALDWGSRGRRFKSCQPDGHASEGPNGPSEAFFV

33

ALDWGSRGRRFKSCQPDGHASEGP+GP+ AF V

1

ALDWGSRGRRFKSCQPDGHASEGPSGPAGAFVV

33

with 3 more sequence matches

56.0%

NZ_CP095274.1:

Lanthipeptides: allorf_2247381_2247521_lanthipeptide

1

ALDWGSRGRRFKSCQPDGHASEGPNGPSEAFFV

33

ALDWGSRGRRFKSCQPDGHASEGP GP+ AF +

1

ALDWGSRGRRFKSCQPDGHASEGPMGPAGAFVI

33

54.0%

NZ_JADKRR010000001.1:

Lanthipeptides: allorf_2147485_2147667_lanthipeptide

1

ALDWGSRGRRFKSCQPDGHASEGPNGPSEAFFVKRAY

37

ALDWGSRGRRFKSCQPDGHASEGP+G ++R Y

1

ALDWGSRGRRFKSCQPDGHASEGPDGSGRG--IRRMY

35

20.0%

NZ_AP022871.1:

Lanthipeptides: allorf_10021069_10021233_lanthipeptide

5

GSRGRRFKSCQPDGHASEG

23

G+RG R SC P G S G

2

GARGARTSSCVPRGSLSGG

20

MIBiG 4.0 matches for allorf_784456_784641

10.0%

BGC0001639.3 (OUH62395.1):

Lasso peptide: klebsidin

23

GPNGPSEAFF

32

G +GP FF

1

GSDGPIIEFF

10

8.0%

BGC0001229.4 (SBI_06990)and 1 other:

BGC0002110.3 (QKV49893.1) Lanthipeptide: birimositide

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

12

KSCQPDGH

19

K CQP+ +

32

KECQPNCN

39

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.96 out of 29.72

Confidence: medium

Additional weak hits (7):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.10 out of 31.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.13 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.69 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.56 out of 26.06

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.44 out of 26.85

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.98 out of 33.14

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

EOG37_RS09890 - Class II

Cleavage pHMM score: -3.60

RODEO score: 0

Monoisotopic mass: 2108.0 Da

Molecular weight: 2109.5 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 2127.5 Da N = 1; 2145.5 Da N = 2

allorf_782985_783149 - Class II

Cleavage pHMM score: -4.90

RODEO score: 0

Monoisotopic mass: 3082.7 Da

Molecular weight: 3084.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3102.7 Da N = 1; 3120.7 Da N = 2

allorf_784456_784641 - Class II

Cleavage pHMM score: -5.70

RODEO score: 0

Monoisotopic mass: 5401.7 Da

Molecular weight: 5405.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 5423.0 Da N = 1; 5441.0 Da N = 2; 5459.0 Da N = 3; 5477.1 Da N = 4

TFBS definitions

Regulator	Description
NtrC	Nitrogen regulatory protein C

NZ_SISU01000003.1 - Region 1 - terpene

Download region GenBank file

Download region SVG

Location: 114,994 - 139,415 nt. (total: 24,422 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EOG37_RS10645: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

EOG37_RS10675: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Hydrolases: hydrolase

EOG37_RS10630: isochorismatase

smcogs: SMCOG1027: isochorismatase

Ligases: synthetase

EOG37_RS10670: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Translocases: transport

EOG37_RS10650: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000644.3	0.63	terpene	carotenoid	Dietzia sp. CQ4
BGC0000637.3	0.58	terpene	carotenoid	Corynebacterium glutamicum
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000648.3	0.56	terpene	carotenoid	Myxococcus xanthus
BGC0000650.3	0.51	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000636.3	0.48	terpene	carotenoid	Brevibacterium linens
BGC0000664.5	0.48	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002309.2	0.46	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000642.3	0.46	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000649.3	0.46	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000644.3	0.63	terpene	carotenoid	Dietzia sp. CQ4
BGC0000637.3	0.58	terpene	carotenoid	Corynebacterium glutamicum
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000648.3	0.56	terpene	carotenoid	Myxococcus xanthus
BGC0000650.3	0.51	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000636.3	0.48	terpene	carotenoid	Brevibacterium linens
BGC0000664.5	0.48	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002309.2	0.46	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000642.3	0.46	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000649.3	0.46	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
tetraterpene	C40 carotenoid		C40	unknown	unknown

Genes with terpene domains:

EOG37_RS10670: PT_FPPS_like

Domain 1 (49 - 332): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

EOG37_RS10675: PT_phytoene_like

Domain 1 (33 - 301): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → diapophytoene; FPP → squalene; FPP → PSPP; GGPP → phytoene; PSPP → hydroxysqualene

EOG37_RS10680: Lycopene_cycl

Domain 1 (4 - 92): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

EOG37_RS10685: Lycopene_cycl

Domain 1 (1 - 107): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (7):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.92 out of 26.33

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.24 out of 20.01

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.61 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.70 out of 29.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.06 out of 28.19

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 19.95 out of 30.98

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.48 out of 28.14

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description

NZ_SISU01000003.1 - Region 2 - azole-containing-RiPP

Download region GenBank file

Download region SVG

Location: 221,907 - 244,196 nt. (total: 22,290 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EOG37_RS11225: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Hydrolases: hydrolase

EOG37_RS11220: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Translocases: transporter

EOG37_RS11200: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002144.3	0.47	ribosomal	phazolicin	Rhizobium sp. Pop5
BGC0002013.4	0.47	ribosomal	curacozole	Streptomyces curacoi
BGC0000569.5	0.42	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0000571.3	0.41	ribosomal	burhizin	Paraburkholderia rhizoxinica HKI 454
BGC0000515.4	0.39	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000845.5	0.39	other (non-nrp beta-lactam)	clavulanic acid	Streptomyces clavuligerus ATCC 27064
BGC0001928.2	0.39	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000188.5	0.39	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0000537.3	0.39	ribosomal	nisin U	Streptococcus uberis
BGC0001789.3	0.39	ribosomal	suicin 90-1330	Streptococcus suis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002144.3	0.47	ribosomal	phazolicin	Rhizobium sp. Pop5
BGC0002013.4	0.47	ribosomal	curacozole	Streptomyces curacoi
BGC0000569.5	0.42	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0000571.3	0.41	ribosomal	burhizin	Paraburkholderia rhizoxinica HKI 454
BGC0000515.4	0.39	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000845.5	0.39	other (non-nrp beta-lactam)	clavulanic acid	Streptomyces clavuligerus ATCC 27064
BGC0001928.2	0.39	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000188.5	0.39	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0000537.3	0.39	ribosomal	nisin U	Streptococcus uberis
BGC0001789.3	0.39	ribosomal	suicin 90-1330	Streptococcus suis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 20.10 out of 21.84

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.82 out of 18.46

Confidence: medium

Additional weak hits (8):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.71 out of 27.78

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.39 out of 30.97

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 22.59 out of 30.97

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.15 out of 32.93

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.35 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.96 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.73 out of 35.63

Confidence: weak

(Thi)azol(in)e containing peptide predictions

(Thi)azol(in)e containing peptide(s)

EOG37_RS11235

Leader:

MSLLMEPTRTTAPEVAPAAGGEAASDALLAEAFGFTITHRGAEEELVMGDVTLCCS

Core:

TTCSSSGSGHQQPR

Core with dehydrations:

DhbDhbCysDhaDhaDhaGDhaGHQQPR

antiSMASH-DB 4.0 matches for EOG37_RS11235

23.1%

NZ_CP012109.1:

Lanthipeptides: allorf_4705695_4705880_lanthipeptide

3

CSSSGSGHQQPR

14

CS +G G + PR

3

CSDAGGGVRTPR

14

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Types:

Type I: Featuring genes encoding indolic acid side ring (series e)
Type II: Featuring the gene coding for quinaldic acid moiety formation (series a, b, and c)
Type III: No genes for synthesizing L-Trp derivatives (series d)

TFBS Finder

Azole RiPPs

TFBS definitions

Regulator	Description
MexT	Global virulence regulator
RpoN	Regulator of nitrogen assimilation and virulence

Core peptide predictions

EOG37_RS11235

Putative Type III

Cleavage pHMM score: 6.10

RODEO score: 30

Monoisotopic mass: 1431.6 Da

Molecular weight: 1432.5 Da

Predicted peptide features:
Central ring: pyridine trisubstituted

Alternative weights: (assuming N unmodified Ser/Thr residues); 1450.5 Da N = 1; 1468.5 Da N = 2; 1486.5 Da N = 3; 1504.6 Da N = 4; 1522.6 Da N = 5; 1540.6 Da N = 6

NZ_SISU01000005.1 - Region 1 - NI-siderophore

Download region GenBank file

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Location: 40,830 - 71,334 nt. (total: 30,505 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

EOG37_RS13840: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

EOG37_RS13860: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

EOG37_RS13880: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

EOG37_RS13910: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: hydrolase

EOG37_RS13825: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

Lyases: decarboxylase dehydratase

EOG37_RS13855: L-carnitine dehydratase/bile acid-inducible

smcogs: SMCOG1189: L-carnitine dehydratase/bile acid-inducible

EOG37_RS13885: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001572.3	0.69	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0001478.5	0.66	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.64	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002305.2	0.60	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0001453.5	0.60	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0000244.4	0.60	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0000940.5	0.60	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0002620.2	0.57	other (other)	fulvivirgamide A2, fulvivirgamide B2, fulvivirgamide B3, fulvivirgamide B4	Fulvivirga marina
BGC0002276.2	0.56	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002073.3	0.53	NRPS	dehydroxynocardamine	Corynebacterium propinquum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001572.3	0.69	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0001478.5	0.66	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.64	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002305.2	0.60	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0001453.5	0.60	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0000244.4	0.60	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0000940.5	0.60	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0002620.2	0.57	other (other)	fulvivirgamide A2, fulvivirgamide B2, fulvivirgamide B3, fulvivirgamide B4	Fulvivirga marina
BGC0002276.2	0.56	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002073.3	0.53	NRPS	dehydroxynocardamine	Corynebacterium propinquum

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 30.88 out of 36.53

Confidence: medium

Additional weak hits (9):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.35 out of 27.55

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.12 out of 21.84

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.23 out of 30.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.78 out of 31.73

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.37 out of 36.54

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.10 out of 25.88

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.88 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.61 out of 35.63

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
DmdR1	Iron(II)-dependent repressor