LOCUS       NZ_CP050705            21610 bp    DNA     circular CON 07-SEP-2024
DEFINITION  Bacillus atrophaeus strain PENSV20 chromosome, complete genome.
ACCESSION   NZ_CP050705
VERSION     NZ_CP050705.1
KEYWORDS    RefSeq.
SOURCE      Bacillus atrophaeus
  ORGANISM  Bacillus atrophaeus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 4148820)
  AUTHORS   Boyetchko,S.M., Jones,S., Yu,M. and Dumonceaux,T.J.
  TITLE     Genome sequence of Bacillus atrophaeus strain PENSV20, a bacterium
            which antagonizes the growth of Sclerotinia sclerotiorum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4148820)
  AUTHORS   Boyetchko,S.M., Jones,S., Yu,M. and Dumonceaux,T.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2020) Bio-products and processes, Agriculture and
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N0X2,
            Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2020
            Assembly Method                   :: SPAdes v. March-2020
            Assembly Name                     :: AAFC_PENSV20_1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019443725.1-RS_2024_09_07
            Annotation Date                   :: 09/07/2024 05:39:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,211
            CDSs (total)                      :: 4,099
            Genes (coding)                    :: 3,930
            CDSs (with protein)               :: 3,930
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 98 of 169
            Pseudo Genes (incomplete)         :: 119 of 169
            Pseudo Genes (internal stop)      :: 23 of 169
            Pseudo Genes (multiple problems)  :: 62 of 169
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:36:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3782312
            Orig. end                         :: 3803922
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050705.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Susan M. Boyetchko.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21610
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="sactipeptide"
                     /region_number="12"
                     /rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /tool="antismash"
     cand_cluster    1..21610
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /kind="single"
                     /product="sactipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21610
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11610](+)"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11610
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
     misc_feature    complement(149..163)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.21"
                     /tool="antismash"
     gene            complement(241..3927)
                     /locus_tag="HCU65_RS18885"
                     /old_locus_tag="HCU65_18930"
     CDS             complement(241..3927)
                     /GO_component="GO:0009325 - nitrate reductase complex
                     [Evidence IEA]"
                     /GO_function="GO:0008940 - nitrate reductase activity
                     [Evidence IEA]"
                     /GO_process="GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1134: flavodoxin"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391609.2"
                     /locus_tag="HCU65_RS18885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18930"
                     /product="nitrate reductase subunit alpha"
                     /protein_id="WP_219946772.1"
                     /transl_table=11
                     /translation="MKKKKMSPLFRRLNYFSPIEHHSNKHSQTTYEDRDWENVYRNRWQ
                     YDKVVRSTHGVNCTGSCSWNIYVKNGIVTWEGQNLNYPSTGPDMPDFEPRGCPRGASFS
                     WYIYSPLRVKYPYVRGVLITMWREALQTHKNPLEAWKSIVEHPEKAKSYKQARGKGGFV
                     RAEWPEVLKLISASLLYTVMKYGPDRNVGFSPIPAMSMISHASGSRFMSLIGGPMLSFY
                     DWYADLPPASPQIWGDQTDVPESSDWYNSGYIITWGSNVPLTRTPDAHFLAEARYKGAK
                     VISISPDFAESSKFADDWLSIRQGTDGALAMAMGHVILQEFYVNQETERFIEYAKQYTD
                     FPFFVTLSKEDGAYTAGRFLHAKDIGRQTEHDQWKPAVWNERTNAFAIPQGTMGSRWDG
                     QKKWNLHMADEDTGEQIEPRLSVLGIEDEIGTVRIPYFSNEGNRVLERDLPIKKLNLNK
                     EDVYVTTVFDLILANYGVNRGIGQKSAASFDDAEPFTPAWQEQVTGIKREMVVKIAREF
                     AQNAIDTDGRSMIIVGAGINHWFNSDTIYRAVLNLVLLVGAQGVNGGGWAHYVGQEKLR
                     PAEGWQTIANAKDWEGAPKLQNGTSFFYFATDQWRYEDQPISDLASPIAASSRYKHHAD
                     YNVLAARLGWLPSYPTFNKNGIDLYKEAEKAGAKTPEDVGAYVAGQLQEKKLKFAIEDP
                     DDEANFPRNLFVWRANLISSSGKGHEYFLKHLLGTTNGLMNDDSDSIRPEEIKWHDQAP
                     EGKLDLLINLDFRMAGTALYSDIVLPAATWYEKHDLSSTDMHPFIHPFAPAISAPWESK
                     SDWDIFKALSKAISDLAEEVEMEPVKEAVATPLLHDTMQEMAQPFGKINDWSKGECEAV
                     PGKTMPNIHVVERDYKQIFYKMTALGPNAALKPSGTKGMSWSIADEYESLKKRLGEITT
                     DSVAKGCPNISEAKQAAEAILTLSSTSNGKVAVKAWESLEDITNLKLKDLAEEREEECF
                     TFEQITAQPKTVITSPAFTGSEKGGRRYSPFTTNVEKLIPWRTLTGRQSYYVDHELMME
                     FGETMATFKPILQHRPFLSKRPDQEGKEIVLNYLTPHNKWSVHSMYFDSLPMLTLFRGG
                     PTVWMNKDDAEDTDIRDNDWIECFNRNGVVVARAVLSHRIPKGMAFMHHAQDRHINVPG
                     TKLTNNRGGTHNSPTRIHVKPTQMIGGYAQLSYGFNYYGPTGNQRDLNVVIRKLKEVDW
                     LED"
     misc_feature    complement(3993..4008)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     misc_feature    4017..4031
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.35"
                     /tool="antismash"
     misc_feature    4021..4036
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 25.74"
                     /tool="antismash"
     gene            complement(4067..4543)
                     /locus_tag="HCU65_RS18890"
                     /old_locus_tag="HCU65_18935"
     CDS             complement(4067..4543)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391610.1"
                     /locus_tag="HCU65_RS18890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18935"
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_003326508.1"
                     /transl_table=11
                     /translation="MNQCDYLLFLKHLPMFNQVSLPIVETLLNNGKFIRCSSDQSPSFL
                     HSQSVYIVLKGSIRFIDSRLPEGSRAIAFWKKGDVFPIDQNGELLLSPFISVKTSEDTL
                     VLEIPFYKFKKIMTYHPQLQTNFLVMLQENLFFSYQLFLRYLHTSQDENVTPDS"
     misc_feature    complement(4601..4616)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.4"
                     /tool="antismash"
     gene            4686..5405
                     /locus_tag="HCU65_RS18895"
                     /old_locus_tag="HCU65_18940"
     CDS             4686..5405
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243773.1"
                     /locus_tag="HCU65_RS18895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18940"
                     /product="YwiC-like family protein"
                     /protein_id="WP_219946773.1"
                     /transl_table=11
                     /translation="MKVLIPKQHGAWAMLLIPFCLGMVKGGPVLWHIPLFTGWLFLYLT
                     VYPITLAVKKKKTKDYQKWIFYYGIAACCSIIAVVIHKPAFIWLCFSLFPLLLVHVYYA
                     RRKNERALLNDIAGVLFFSTGGLASCWLGAGSLDGWAWFIFAQSSLFFIGSSFYVKSMI
                     REKNNKTFAYCSWGYHLLLPFLSISLGASWGALAFIPSSLRAWFLHGKERSIKTIGILE
                     IVNACFFFAVICIFLTR"
     gene            complement(5445..6161)
                     /locus_tag="HCU65_RS18900"
                     /old_locus_tag="HCU65_18945"
     CDS             complement(5445..6161)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391612.1"
                     /locus_tag="HCU65_RS18900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18945"
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_003326506.1"
                     /transl_table=11
                     /translation="MNFLSVRKSDKDLLSQDLHQLLESISTKRKIEKNTFLFQEGMDAK
                     ELYLIQSGLIEIGKLTSDGKDLTLRICQKNDIVGELTLFTEEPRYMLSAKVLADGEVFV
                     INKNKLEKELVQNGALTFEFMKWMSTHLRKIQSKIRDLLLHGKKGALYSTLIRLSNSYG
                     VKRSDGILINIALTNHDLAKFCAAARESVNRMLGDLRKQGIISIEESGKIVLHQRDYLR
                     CEIECENCPLDICNID"
     gene            complement(6272..7459)
                     /locus_tag="HCU65_RS18905"
                     /old_locus_tag="HCU65_18950"
     CDS             complement(6272..7459)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391613.2"
                     /locus_tag="HCU65_RS18905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18950"
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="WP_003326505.1"
                     /transl_table=11
                     /translation="MINRQNIQLSLQSCSLVAGFMVWVLISSLISQMSSDINLSAGDIS
                     LVTAIPVILGSLLRIPLGYYTNRFGARMTFMISFLLLLFPVFWISIADSVFDLAVGGFF
                     LGIGGAVFSIGVTSLPKYYPKEKHGFINGIYGAGNIGTAITTFAAPVVAQSVGWKSTVQ
                     MYMVLLALFALLHFFLGDRNEKKVKVSVKVQIKAVYRNHLLWFLSLFYFITFGAFVAFT
                     IYLPNFLVQHFGLSPVDAGFRTAGFIAVSTLLRPVGGFLADKVSPLRMLMYVFAGLTLS
                     GIILSFSPSLGIYAFGALTVAVCSGIGNGTVFKLVPFYFSKQAGIANGIVSAMGGLGGF
                     FPPLILASVFQATGQYAIGFMALSEAALASFVIVIWMYWQERMKTHTERNGHSVN"
     misc_feature    complement(7524..7539)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     misc_feature    7542..7555
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.96"
                     /tool="antismash"
     misc_feature    7543..7556
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.22"
                     /tool="antismash"
     gene            complement(7593..9263)
                     /gene="argS"
                     /locus_tag="HCU65_RS18910"
                     /old_locus_tag="HCU65_18955"
     CDS             complement(7593..9263)
                     /EC_number="6.1.1.19"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="argS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391614.1"
                     /locus_tag="HCU65_RS18910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18955"
                     /product="arginine--tRNA ligase"
                     /protein_id="WP_219946774.1"
                     /transl_table=11
                     /translation="MNIAEQMKDVLKEEIKAAVLKAGLAEENQIPNVILETPKDKTHGD
                     YSTNMAMQLARNAKKAPRQIAEDIVASFDKGKASIEKLDIAGPGFINFYMDNQYLTKLI
                     PSVLEAGEAYGETNIGNGERIQVEFVSANPTGDLHLGHARGAAVGDSLCNVLAKAGYDV
                     SREYYINDAGNQINNLALSVEVRYFEALGLEKEMPEDGYRGEDIIAIGKRLAEEYGDRF
                     VKEEESERQKFFREYGLKYELEKLRVDLENFRVPFNVWYSETSLYENGKIDKALEALRK
                     QGHVYEEDGATWFRSTTFGDDKDRVLIKKDGTYTYLLPDIAYHKDKLDRGFDKLINVWG
                     ADHHGYIPRMKAAIEALGYQKGTLEVEIIQLVHLYKNGEKMKMSKRTGKAVTMRDLIEE
                     VGLDAVRYFFAMRSADTHMDFDLDLAVSTSNDNPVYYAQYAHARICSMLRQGEEQGLTP
                     AADLDFSAVNSEKEYDLLKTIGGFPEAVAEAAEKRIPHRVTNYIYDLASSLHSFYNAEK
                     VIDPENEEKSRARLALMKATQITLNNALQLIGVSAPEKM"
     gene            complement(9260..9688)
                     /locus_tag="HCU65_RS18915"
                     /old_locus_tag="HCU65_18960"
     CDS             complement(9260..9688)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391615.1"
                     /locus_tag="HCU65_RS18915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18960"
                     /product="DUF1934 domain-containing protein"
                     /protein_id="WP_003326502.1"
                     /transl_table=11
                     /translation="MKQETPITLHVKSVIADDGKEEVIEFRTSGSYHVKQNKVYLSYYE
                     EHDLGKVKTIVKVSESEVFVMRSGAVKMNQRFVPGASTIAKYKMSFGELELTTSTKSIQ
                     SDIGKERGRISIAYDMHVGDEQQHLHNMTITYEGGLDA"
     misc_feature    9868..9882
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.54"
                     /tool="antismash"
     misc_feature    9894..9909
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            10001..10132
                     /gene="sboA"
                     /locus_tag="HCU65_RS18920"
                     /old_locus_tag="HCU65_18965"
     CDS             10001..10132
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /gene="sboA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: Subtilosin_A"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391616.1"
                     /locus_tag="HCU65_RS18920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18965"
                     /product="subtilosin A"
                     /protein_id="WP_003222002.1"
                     /sec_met_domain="Subtilosin_A (E-value: 8.9e-29, bitscore:
                     97.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKAVIVENKGCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
     CDS_motif       10001..10030
                     /prepeptide="leader"
                     /note="peptide class: sactipeptide"
                     /note="predicted sequence: MKKAVIVENK"
                     /locus_tag="HCU65_RS18920_sactipeptide"
                     /aSTool="sactipeptides"
                     /tool="antismash"
     CDS_motif       10031..10132
                     /prepeptide="core"
                     /locus_tag="HCU65_RS18920_sactipeptide"
                     /peptide="sactipeptide"
                     /predicted_class
                     /score="33.00"
                     /molecular_weight="0.0"
                     /monoisotopic_mass="0.0"
                     /core_sequence="GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
                     /aSTool="sactipeptides"
                     /tool="antismash"
                     /leader_sequence="MKKAVIVENK"
                     /leader_location="[10000:10030](+)"
     gene            10264..11610
                     /gene="albA"
                     /locus_tag="HCU65_RS18925"
                     /old_locus_tag="HCU65_18970"
     CDS             10264..11610
                     /codon_start=1
                     /gene="albA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: subtilosin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Subtilosin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thurincin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SPASM"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391617.1"
                     /locus_tag="HCU65_RS18925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18970"
                     /product="subtilosin maturase AlbA"
                     /protein_id="WP_003326501.1"
                     /sec_met_domain="subtilosin (E-value: 0.0, bitscore:
                     1054.2, seeds: 6, tool: rule-based-clusters)"
                     /sec_met_domain="Subtilosin_rSAM_RRE (E-value: 5.3e-27,
                     bitscore: 92.0, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 5.6e-07, bitscore: 27.7,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Thurincin_rSAM_RRE (E-value: 3.5e-08,
                     bitscore: 31.3, seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 2.8e-07, bitscore: 28.1,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="PTHR43273 (E-value: 1.3e-43, bitscore:
                     147.3, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.3e-21, bitscore: 75.6,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 3.3e-10, bitscore:
                     38.1, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 2.2e-08, bitscore: 32.4,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLIERMFPFISESVRVHQLPEGGVLEIDYMRDNVSISDFEYLDLN
                     KTAYELCMLMDGQKTAEQILEYQCAAYNESPEDHKDWYYEMLDMLLNKQVIRLTDQPEY
                     RRIATSGSSDFPMPLHATFELTHRCNLKCAHCYLESSPEALGTVSLEQFKKTADMLYEK
                     GVLTCEITGGEIFVHPNANELLEYVLKKFKKVAVLTNGTLMRKESLEILRAYKQKIIVG
                     ISLDSVHSEVHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKV
                     RDLGAKAFSYNWVDDFGRGKDMIHPTKDAEQHRKFMEYEQNVIDEFKDLIPIIPYERKR
                     AANCGAGWKSIVISPFGEVRPCALFPKEFSLGNIFHDSYESIFDSALVHKLWKAQAPRF
                     SEHCKKDKCPFSGYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI"
     aSDomain        10279..10557
                     /aSDomain="Subtilosin_rSAM_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing rSAM protein in a subtilosin
                     sactipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_HCU65_RS18925_Subtilosin_rSAM_RRE.1"
                     /evalue="3.60E-29"
                     /identifier="RREFam018.1"
                     /label="HCU65_RS18925"
                     /locus_tag="HCU65_RS18925"
                     /protein_end="98"
                     /protein_start="5"
                     /score="92.0"
                     /tool="antismash"
                     /translation="MFPFISESVRVHQLPEGGVLEIDYMRDNVSISDFEYLDLNKTAYE
                     LCMLMDGQKTAEQILEYQCAAYNESPEDHKDWYYEMLDMLLNKQVIRL"
     gene            11623..11784
                     /locus_tag="HCU65_RS18930"
                     /old_locus_tag="HCU65_18975"
     CDS             11623..11784
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391618.1"
                     /locus_tag="HCU65_RS18930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18975"
                     /product="bacteriocin biosynthesis protein AlbB"
                     /protein_id="WP_003326500.1"
                     /transl_table=11
                     /translation="MSPTQRKWLLYILSFVFVIGAVVYFVKSDYLFTLISIVIAIIFGM
                     RARKAESR"
     gene            11781..12500
                     /locus_tag="HCU65_RS18935"
                     /old_locus_tag="HCU65_18980"
     CDS             11781..12500
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391619.1"
                     /locus_tag="HCU65_RS18935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18980"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_219946775.1"
                     /transl_table=11
                     /translation="MSMLDIHALSVWYEQDNVILENVDLHLEKGAVYGLLGVNGAGKTT
                     LINTLTGVNRNFSGSFKLCGIEVNAKTPLKTLDQLKTNRYFAADYPLLFTEITAKEYVS
                     FVHSLYQKEFHEQQYVSLAEEFHFSQYMNRRISELSLGNRQKVVLLTGLLLRAPLFILD
                     EPLVGLDVESIEVFYEKMQEYCKSGGTILFSSHLLDVVKRFCHYAAILHDKQIRKLIPI
                     KDDTDLRREFFEVIGHE"
     gene            12493..13821
                     /locus_tag="HCU65_RS18940"
                     /old_locus_tag="HCU65_18985"
     CDS             12493..13821
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003326498.1"
                     /locus_tag="HCU65_RS18940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18985"
                     /product="bacteriocin biosynthesis protein AlbD"
                     /protein_id="WP_219946776.1"
                     /transl_table=11
                     /translation="MNNVFQILTLLFKQLYGRQGKKDAIRITAGLAVLALFEIGLIRQA
                     GIDESVLGKTYVTLALLFMNVYMVFLSVSSQWKESYMKLSCLLPISSRSFWLAQSVVLF
                     VDACLRRTLFFFILPLFLFGTGTLSGTETLFWLGKFSFFTVYSILLGVLLSNLFVKKKG
                     FVFLLHAAAFACICASVVFMPALSIPLCGVHMAWGVFFDFPSFLQVPDQQNKMHSFMRT
                     SEFSFYKREWNRFISSKAMLLNYIVMAAFSGFFSFQMMKTGIADQHIIYIVITALLLIC
                     SPIALLYSLEKNDRMLLITLPIKRRTMFWAKYRFYSGLLAGGFILIAMIVGLISSQPIS
                     FMTFLQCIELLLMGAFIRLTSDEKRPSFGWQTEQQLWSGFSKYRSYVFCIPLFLATIAG
                     TVISLAIIPIAGLIIVYLLQKQEGGFFDTRRKSERFGNEPVND"
     gene            13769..14959
                     /locus_tag="HCU65_RS18945"
                     /old_locus_tag="HCU65_18990"
     CDS             13769..14959
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003326497.1"
                     /locus_tag="HCU65_RS18945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18990"
                     /product="insulinase family protein"
                     /protein_id="WP_309143173.1"
                     /sec_met_domain="Peptidase_M16_C (E-value: 2.1e-06,
                     bitscore: 25.8, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQGEKVNDLEMNLLTTDQFSTISIAASFLKPIEIEAEPEEETIY
                     FYGAAAYLKQNITDVFGYAAGSRFIYSANLFFDRQLKTYGTRLIHPLYHKNLNIDDVLE
                     TFVDRSSLPASLPADIAETAKADLLLKIEKKFADPFSYSAARLAEETFGSPMYGTAMFG
                     RKEKVQAIQPKRFLDASDFKTDLLSQQKQLYVIGNVQELHAGGYSRHAPSMNGRRKPVN
                     KNIYETETRSTAGPSVMTLGFDCGEMNGFRDYIKIQLIDGLLGKYGHSALFRHFREKQM
                     AVYHVITRYDIMSNLLLVSVCTNRVQEKEIPPRVIETVMNFHVDEQELEKAKQFLKNEI
                     LLQLDSPEGLLAYMGILRHYSYKKEDILDGISTITCRDLLQYVTNIKYIGAHVVRG"
     gene            14965..16242
                     /locus_tag="HCU65_RS18950"
                     /old_locus_tag="HCU65_18995"
     CDS             14965..16242
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483836.1"
                     /locus_tag="HCU65_RS18950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_18995"
                     /product="pitrilysin family protein"
                     /protein_id="WP_219946777.1"
                     /sec_met_domain="Peptidase_M16 (E-value: 8.7e-13, bitscore:
                     46.4, seeds: 24, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKETFFHQLDARTEITYADNGLKICRVKFPNAHMRLCNVKVNFG
                     SRYIRLHSGDSEKVLPYGTAHFLEHVLFWHNGRNLYSDFFDHGALLNAFTTYTDTNFMF
                     TSLPDRLEHTIPKLLDALWNPSFEENIVAQEKSVIKSEIETAHLNQQLHYHYQMLNLLS
                     PASPAAIFPAGRTEDIEALTINDLKEAYATGYQPQRMTMFLIGGSEDTETILPSHLRLR
                     GRSGHTAKRKFMPACPPFLQNQSLGDAECSGETWTGLHIGALPDKNDLFTLQMYWDIVS
                     RVLFHIDSPLFQEIRQSRLLNIQSLSAESHVSEDGGFLILHSKGAHSSAYIDVAANYVM
                     QQKCQAEAWLEYGKESLLNAIIYDSDYVRKCFEWAAECERYDCSFFELYRLIQNMTVQD
                     FLYLIDTVASAKKATIHVSHPEVVGQ"
     gene            16239..16940
                     /locus_tag="HCU65_RS18955"
                     /old_locus_tag="HCU65_19000"
     CDS             16239..16940
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250988.1"
                     /locus_tag="HCU65_RS18955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_19000"
                     /product="bacteriocin biosynthesis protein AlbG"
                     /protein_id="WP_219946778.1"
                     /transl_table=11
                     /translation="MKKSTVFTVLLLLMVMAAYSFGWFQASAAAAGQYVQLIKNDYVRL
                     GLLSCAASVLMLPAFLYLHYVTRSVKSMTAAFQKITQSHQSCCDFQHRKLCSRYAGDVK
                     SLRESYKGIRQTYVMGAVLCQVIVFGYMFEVVKSTPFSFNAPPAVTKGIAIVLIIYLLF
                     YMRSYLLQLLQNGQTAKKAVVLILAGAGIWWMLSFTISELLFLIILAVIQQIGSFIYKR
                     VSYRSSAPLDL"
     gene            complement(16947..18323)
                     /locus_tag="HCU65_RS18960"
                     /old_locus_tag="HCU65_19005"
     CDS             complement(16947..18323)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012118684.1"
                     /locus_tag="HCU65_RS18960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_19005"
                     /product="YncE family protein"
                     /protein_id="WP_219946779.1"
                     /transl_table=11
                     /translation="MKKNDLSAFQENCFCFCDEEISREALFQVPIDIPEGFTIDSAEAA
                     AAVTWSTDNLSCISEPCLIQTGPEPEDIGVQYAVRLQGTVTLLVSVSPVRNQYGQGDGA
                     VSVIHNAEIDQVVYYSDNPDDCPDFRDITIENLVVVPPFYGSPLSVTGTIVLVPAPDQR
                     SYVFTANTGDSTVSVIDTDVNTLVSTISLSDIPTDLGVTLDKAYTYVLHRNANLVSIIN
                     NETLTVTQTIPLAGAPRKIQFETGGGFAYILAGGSSIYEINISSQTITNTFTVSPAADD
                     FALDPNGQFFYTTNSVSWVVNKYDINTGQLIDSLSNFETPNLIETPLIGDFAYLENGEL
                     RPNGVSVIDLITFSESSKINRLFDGLITIVFSADGTRAFFLEPYFEVFPENLTVVDTAQ
                     HTFITTASLPGASDLAVTPDNQYIYVAQPAANTVTVYRTSDYTAVAVIPVGAGPSAISI
                     "
     gene            complement(18365..19726)
                     /locus_tag="HCU65_RS18965"
                     /old_locus_tag="HCU65_19010"
     CDS             complement(18365..19726)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003326493.1"
                     /locus_tag="HCU65_RS18965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_19010"
                     /product="YncE family protein"
                     /protein_id="WP_219946780.1"
                     /transl_table=11
                     /translation="MKKIEHSFKEKAVRQQEDCLCFCEEEASREAMFQAPIEVPEGFVV
                     DPAEAVANVTWNADSLSCVSERCFIQTGPGPDEIEIHFAVRLQGTITLLVSVSPVRNQY
                     GQGEGAVSVIHNVEIDQVVYYSDEPDDCPDFSDITIENLVVVPPFYGSPLSVTGTIVLA
                     PEPEPEPVYAFTANPDDQSVSVIDTNTHTVITTISLPYEPTGIEITPDKASTFVLHFNN
                     SVISVIDNGTLTVTGSIPLDQPPETIKFMPEHDFAYVLAGNAIYVIRIETSAVERSIPV
                     EGYDIAIDPNGLFAYVLDFGLVQKVDLSTGEVTGMIERELIVNSIETKPPERYAYVLEQ
                     DHFLNYLTIIDLDTFTISSTQELEYEGEYQMFKSGSGLYLFDGFTHNLYSVSPSGAGVI
                     GNLPQSARDFAFTPNGEFLYATRFIEQSIIVYNTDDYSVETVISLGVSPSDIAI"
     gene            20009..20563
                     /locus_tag="HCU65_RS18970"
                     /old_locus_tag="HCU65_19015"
     CDS             20009..20563
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003326492.1"
                     /locus_tag="HCU65_RS18970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HCU65_19015"
                     /product="YdhK family protein"
                     /protein_id="WP_088118182.1"
                     /transl_table=11
                     /translation="MKRNTTLIGIVSLLLLLMLSACGSDNQDKKSDQTMNMDDTHTHSE
                     SSEIPEGLTAAQHPNYKVGSQAIIKADHMKGMKGAEATITGAYDTTAYAVSYTPTTGGT
                     RVENHKWVIQEEIKNASDETLKPGTDVIIEADHMKGMKGATGKIDSAEKTTVYTVDYTP
                     TTGGEKVKNHKWVTESELSSK"
     misc_feature    20725..20741
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 22.81"
                     /tool="antismash"
     misc_feature    21136..21149
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.67"
                     /tool="antismash"
     misc_feature    complement(21157..21170)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 20.42"
                     /tool="antismash"
     misc_feature    21453..21465
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 19.83"
                     /tool="antismash"
ORIGIN
        1 agacggttag ttttcggtcc tgatttcagc tcaagctttc ctttttttaa tgtccagccg
       61 cctttatatt tgtcttggtc ctcccactgc ttcggatagc cgattccagg ttttgtttca
      121 acattattga aatacatata ttccgctccg gaacggtttg tccacgtgtt tttgcaggtc
      181 acgctgcatg tgtggcagcc gatgcattta tctaaattca tgaccattcc gatttgcgct
      241 ttaatcttca agccaatcga cctccttcag cttgcggatg accacattca gatcacgctg
      301 attcccagtt gggccatagt aattaaatcc gtagctaagc tgagcatagc cgccgatcat
      361 ttgtgtcggt ttaacatgaa tccttgtcgg gctgttatgg gtgccgccgc ggttattcgt
      421 gagttttgtg ccgggtacgt tgatgtggcg gtcttgtgca tggtgcataa acgccatccc
      481 tttaggaata cggtgagaaa gaacggctct cgctacgaca acgccatttc ggttgaagca
      541 ttcaatccaa tcgttgtctc tgatatctgt gtcctctgca tcatctttat tcatccacac
      601 tgtcggccct ccgcggaata gcgtcagcat aggtaatgaa tcaaagtaca tgctgtggac
      661 ggaccatttg ttatgcggcg ttaaataatt gaggacgatt tctttgccct cttgatcagg
      721 ccgtttactc agaaacggac gatgctggag gatcggttta aatgtcgcca tcgtttcccc
      781 gaactccatc atcagctcat gatcgacata gtaagactgt ctgcccgtca gcgtccgcca
      841 agggattaat ttttccacat ttgtcgtaaa cggcgagtac ctgcgtcccc ctttttctga
      901 gcctgtaaat gccggagacg tgatgaccgt ttttggctga gcggtgattt gttcgaacgt
      961 aaagcattct tcttcccgct cttcagccag gtcttttaac ttcaggtttg tgatgtcttc
     1021 aagtgattcc catgctttca ctgcaacttt tccattcgaa gtggaagaaa gggttaaaat
     1081 ggcttctgcc gcctgcttcg cctcacttat attcggacat cccttggcga cgctgtccgt
     1141 tgtgatttct cccagtctct ttttgagtga ttcatattca tcggctatcg accagctcat
     1201 cccttttgtt ccgcttggtt ttagggcggc gttagggccg agtgcggtca ttttataaaa
     1261 gatttgttta tagtcccgtt caacaacatg aatgttcggc atcgttttcc ccggaacggc
     1321 ttcgcattcg cctttgctcc agtcattgat ttttccgaaa ggctgcgcca tttcctgcat
     1381 agtgtcatgg aggagcggcg tcgcgacagc ttctttaact ggctccatct ctacttcttc
     1441 agccaagtcg gatatggctt ttgataatgc tttaaaaata tcccagtctg acttggattc
     1501 ccaaggcgct gagatggccg gagcaaaagg atgaataaac ggatgcatat ctgtactgct
     1561 gagatcatgt ttttcatacc aagtcgccgc cggaagcacg atatcggaat acaatgccgt
     1621 tccagccatt cgaaaatcga gattgattaa taagtcgagc tttccttccg gtgcttgatc
     1681 atgccatttg atttcttccg gacggatgct gtcgctgtcg tcattcatca atccgtttgt
     1741 cgtccccaag agatgcttaa gaaaatattc atgccctttt cccgaacttg agatcagatt
     1801 cgctctccat acaaaaagat ttctcgggaa attggcttca tcgtcaggat cttcaatggc
     1861 aaatttcagt tttttctctt ggagctgacc ggccacatat gcaccaacat cttcaggcgt
     1921 ttttgcaccc gctttttcag cttccttgta aagatcaatt ccgtttttat taaacgtcgg
     1981 gtaagacggg agccacccta atcttgccgc cagcacattg tagtcggcgt gatgcttgta
     2041 gcgggaggat gctgcgatcg gagaagccaa gtcactgatc ggctgatcct cgtagcgcca
     2101 ctggtctgtc gcaaagtaga agaatgatgt gccgttttgc agctttggcg ccccctccca
     2161 gtcctttgca ttggcaatcg tctgccagcc ttcagccggg cgaagctttt cctgccccac
     2221 atagtgagcc catccgcccc cgtttacgcc ttgggcgccg actaataaaa caagatttaa
     2281 aaccgcccgg taaatcgtgt cagagttgaa ccaatggtta attcccgccc ctacgataat
     2341 cattgaccgt ccgtctgtat cgatcgcatt ttgggcaaat tctctggcaa ttttaacgac
     2401 catctccctt ttgatccctg ttacctgttc ttgccaggcg ggtgtaaacg gctcagcatc
     2461 atcaaacgag gcggctgatt tctgcccgat tccccgattg acgccatagt ttgccagaat
     2521 taaatcaaaa acggttgtga catatacgtc ttccttattc aggtttagtt tcttaatggg
     2581 aagatcgcgc tcaagcactc tgtttccctc atttgagaaa taagggattc gaactgtgcc
     2641 gatttcgtct tctattccga gcactgacag cctcggttca atttgttccc ctgtatcttc
     2701 atctgccatg tgcagattcc atttcttttg gccgtcccat cgtgatccca ttgttccttg
     2761 cggtatcgca aatgcgtttg tacgttcatt ccacacagca ggcttccact gatcatgctc
     2821 tgtttgccgt ccgatgtcct tcgcatggag aaaacgtccg gctgtataag cgccatcttc
     2881 tttagacaag gtgacaaaga aagggaaatc cgtgtattgc tttgcgtact ctatgaaacg
     2941 ctctgtttct tgattcacgt aaaattcttg caggatgaca tgccccattg ccatcgcaag
     3001 cgcgccgtct gttccctgac ggatgctcag ccagtcatcc gcaaattttg acgattccgc
     3061 aaaatccgga ctaattgaga tgacttttgc ccctttatag cgggcttctg ccaaaaagtg
     3121 cgcatccggc gttcttgtta acggcacatt ggagccccat gtgatgatat agcccgaatt
     3181 gtaccagtca ctgctttccg gcacgtctgt ctggtctccc cagatttgcg gagacgctgg
     3241 gggaagatcc gcataccaat cataaaagct cagcataggg ccgccgatta atgacataaa
     3301 ccgcgagcct gacgcgtggc tgatcattga catggcggga atcggagaaa atccgacgtt
     3361 gcggtcaggg ccgtatttca tgactgtata taatagagaa gcagaaatca gtttcagcac
     3421 ctccggccac tcagcgcgca caaaaccgcc ttttcctctc gcctgtttat aggacttcgc
     3481 tttttccgga tgctcaacga tagatttcca tgcttccaat ggatttttgt gtgtttgcaa
     3541 tgcttcccgc cacatagtaa tcagcacacc gcgaacgtat ggatatttga cacgcagcgg
     3601 gctgtagata taccaggaaa aacttgcccc gcggggacag ccccttggct cgaagtcagg
     3661 catatcagga ccggttgaag gataattcaa attttgccct tcccacgtga cgatgccgtt
     3721 tttcacataa atattccagc tgcaagatcc cgtgcagttg actccgtggg tggagcggac
     3781 aactttatcg tactgccatc tgtttctata tacattctcc caatcgcgat cttcgtatgt
     3841 tgtttgacta tgtttattag aatgatgctc aataggagag aaatagttta gtctcctgaa
     3901 cagcggactc attttcttct ttttcatcga tgcttcactc ctttgagtca ggtgattctc
     3961 atttcactat atacagaggc agcatcattc cttgtgaact ttgtcacata cgcccctttt
     4021 ttcacaaatt tttcacattc aggattcaaa aaaacccggt tcctggttag gaatcgggag
     4081 tgacgttttc atcctgagat gtgtgcagat aacgcaaaaa taactggtag gaaaaaaaga
     4141 gattttcctg aagcatgacc agaaaattcg tctgcagctg cggatgatag gtcattattt
     4201 ttttaaattt gtaaaaagga atttccaaca cgagtgtgtc ttcagatgtt ttgactgaaa
     4261 tgaaaggaga taaaagcagc tcgccatttt gatcaatggg aaacacatca ccttttttcc
     4321 aaaatgcaat ggcgcgcgaa ccttcaggca gtctgctgtc aataaaacga atgctgcctt
     4381 ttaatacaat ataaactgat tgtgaatgca aaaaagatgg agactgatca gatgagcaac
     4441 ggatgaactt cccgttattc agcaatgttt cgacaatggg aagagatact tgattaaaca
     4501 ttggaagatg tttcaaaaat aataaatagt cacactgatt cataggaaag cctcctggga
     4561 taaggatagc tttagtgtat cttgattgaa tcaatacagc agtgatgtaa ttcacagaaa
     4621 ttatgataaa tgtcacttat cggaagagtg attgctttta taatgaaaca gaaaggagtg
     4681 tttcaatgaa ggttctcatt ccgaagcagc acggtgcatg ggctatgctg ttgattccgt
     4741 tttgcctcgg tatggtgaag ggaggtcccg ttctatggca tatcccgcta tttacgggct
     4801 ggctatttct ctatttgacc gtttacccca ttaccttggc ggtgaaaaag aaaaaaacaa
     4861 aagactatca aaaatggatt ttttattacg gcattgcagc ttgctgctct atcattgcgg
     4921 ttgtcatcca taagcctgcg ttcatctggc tctgcttttc tttgtttccg ctgctcctcg
     4981 ttcatgtcta ttatgcccgc agaaaaaacg aaagagcgct tctgaatgat attgcgggag
     5041 tcttgttctt tagtacaggc ggacttgcca gctgctggct gggggcggga agccttgacg
     5101 gctgggcatg gtttattttc gcgcaatcca gcttattttt tatcggaagt tccttttatg
     5161 tgaagtctat gatccgggag aaaaacaaca aaacatttgc atactgttca tggggatatc
     5221 acctgcttct tccctttctt tctatatctc tcggagcaag ctggggggct ctcgctttta
     5281 ttccaagcag tttgagagca tggttcttac acggaaagga gcgatctatt aagacgatcg
     5341 gcattcttga aatcgtgaac gcgtgcttct ttttcgcagt catatgtatt tttttaacaa
     5401 gataaaacaa ggactgaagt ttgcagtcct tgtttatttc ttgattaatc gatattgcaa
     5461 atatccagcg ggcaattttc gcactcaatt tcacatctta aatagtcccg ctgatgcaaa
     5521 acaatctttc cggattcctc aattgaaatg attccttgtt tacgaagatc acccagcatg
     5581 cgattgacac tctctcttgc agccgcacaa aacttcgcca aatcatggtt cgtcagtgca
     5641 atgtttataa gaattccgtc gcttcgtttc accccatagc tatttgacaa acgaatgagt
     5701 gtggaataaa gcgccccttt tttaccatga agaagaaggt cacggatttt ggactgaatt
     5761 tttcgcaggt gggtgctcat ccatttcata aattcaaatg tcaaagcacc gttttgaacc
     5821 aattcctttt ccagtttgtt tttattaatg acgaacactt caccgtctgc taacactttt
     5881 gcactcagca tatatctcgg ttcttctgta aaaagtgtta attccccgac aatatcattt
     5941 ttttggcaga tcctgagcgt cagatccttg ccgtcagacg tcagtttgcc gatttcaatg
     6001 agtcctgatt gaatcagata aagctctttt gcatccattc cttcttgaaa cagaaacgtg
     6061 tttttttcaa tttttctttt tgtactgatt gattccagca attgatgcag atcttgtgag
     6121 agaagatcct tatcagattt tcgaacagag agaaaattca tgagctcacc tcgaagatag
     6181 tcacgttgtg tatttctatt gtaacgtgaa tcgatttgca tcattttaaa aaaacttaca
     6241 atctcgtgaa ttccctaaaa gccgcttccc attagtttac gctgtgccca tttctctcag
     6301 tgtgcgtttt catcctttcc tgccaataca tccagatgac aatgacaaag ctggctaaag
     6361 cagcttccga gagcgccata aatccgattg catattgtcc tgtcgcttga aatacgcttg
     6421 ccaggatcag tggcggaaag aatcctccca atccccccat ggcagaaaca ataccgtttg
     6481 caattcccgc ttgttttgag aaataaaaag gcaccaattt aaaaactgtt ccgtttccaa
     6541 ttcctgagca gacagcaact gttaacgccc caaaagcgta aatgccgaga ctcggtgaga
     6601 acgacaagat aatgcccgat aatgtcagac ccgcgaagac gtacattaac attctaagcg
     6661 gactcacttt atctgccaaa aatccgccaa caggtctgag aagcgtggac acagcaataa
     6721 aacctgccgt tctaaacccg gcatccacag gactaagacc aaagtgctga acaagaaagt
     6781 taggaagata aatggtaaag gcaacgaacg cgccaaatgt tataaaataa aaaaggctta
     6841 agaaccacaa cagatgattt cggtaaacgg cttttatttg aacctttacg gaaactttga
     6901 ctttcttctc gtttcgatcg cccaggaaaa agtgcagcaa agcaaataaa gccagcagga
     6961 ccatatacat ttgcaccgta ctcttccacc ccacagattg agcaacaaca ggagctgcaa
     7021 acgttgtgat ggcggttccg atatttccag cgccgtaaat gccgtttata aaaccgtgtt
     7081 tttccttagg ataatacttc ggaagagatg tcaccccaat ggagaaaacc gctcctccaa
     7141 tccccaagaa aaaaccgccc acggccaaat caaacacaga atctgcgata ctgatccaaa
     7201 acacaggaaa caaaagcaat agaaagctga tcataaaagt catccgcgcg ccaaacctgt
     7261 tcgtgtaata tcccaaagga atgcggagaa gtgaccccag aatgacaggt atggccgtaa
     7321 ccagcgaaat gtcgccggcg ctcaaattaa tatcagatga catttgagaa attaatgaag
     7381 aaatcaaaac ccaaaccata aatcctgcca ccaagctgca tgactgtaac gataattgaa
     7441 tgttttgacg gttaatcatt cggcttgccc ctttcaatga ttcaataatc agtcttatca
     7501 tacaaaaact cggtgaatag gattgtgaag tacatcacac gttttttaaa caaaaaagga
     7561 caaaaccccg gggctttgtc ctttcatctt cattacattt tttcaggagc cgaaacgcca
     7621 atcaactgaa gcgcattatt cagcgtgatt tgggttgctt tcattaaagc taagcgtgcg
     7681 cggcttttct cttcattttc aggatcgatt accttttccg cattatagaa gctgtgcaga
     7741 gaagatgcca agtcataaat atagttcgtc acgcggtgcg gaatgcgttt ttccgccgct
     7801 tccgccactg cttcagggaa gccgccgatc gttttcagca aatcgtattc tttttcagaa
     7861 ttgacggcgc taaagtcaag atcagccgca ggcgttaatc cctgctcttc tccttggcga
     7921 agcatgctgc agattctcgc gtgagcgtat tgcgcgtaat acaccggatt gtcatttgaa
     7981 gtggacaccg caagatccaa gtcaaaatcc atatgtgtat cggcactgcg catcgcgaag
     8041 aaataacgga cggcgtctaa gccgacttct tcaatcaagt cgcgcatcgt taccgccttt
     8101 cctgtacgct tgctcatttt cattttctcg ccgtttttat atagatgcac gagctgaatg
     8161 atttctactt ctaacgttcc tttttgatag ccaagcgcct caatggccgc tttcatccgc
     8221 ggaatatagc cgtggtgatc tgcgccccag acgtttatca gcttatcgaa cccgcggtct
     8281 agcttgtcct tatgataagc gatatcaggc agcaaataag tgtaagtgcc gtctttttta
     8341 atcaaaacac ggtctttgtc atcgccgaat gttgttgagc ggaaccaagt tgcgccgtct
     8401 tcttcataca cgtggccttg tttacgcagc gcttcgagcg ccttgtcgat ttttccgttt
     8461 tcgtataatg acgtttcaga ataccagaca ttaaacggca cgcggaagtt ttctaagtct
     8521 acacgaagct tttccaactc atacttcaaa ccgtattccc ggaagaactt ctgtctttcg
     8581 ctctcttctt ctttaacaaa gcggtctccg tattcttcag cgagacgctt cccgattgcg
     8641 ataatatcct cgccgcggta tccgtcttca ggcatttctt tttcaaggcc gagcgcttca
     8701 aagtaacgga cttcgacaga aagagccaag ttgttgatct gatttcccgc gtcattgata
     8761 taatattcgc ggctcacatc atatccggct ttagccagca cattgcacag agaatcgccg
     8821 acggctgctc cgcgcgcatg gcctaaatga agatcgcccg tcggattcgc agatacgaat
     8881 tccacttgga tgcgctcgcc gtttccgata tttgtttccc cgtatgcttc ccccgcttcc
     8941 aatacagatg ggatcagctt cgttaaatat tgattatcca tatagaaatt gataaatcca
     9001 gggcctgcga tatcaagctt ttcaattgaa gcctttcctt tatcaaaaga agctacaata
     9061 tcctcagcga tttggcgcgg ggcttttttt gcgtttcttg ccagctgcat cgccatgttc
     9121 gttgaataat caccatgtgt tttatctttc ggtgtctcta aaatgacatt tggaatctgg
     9181 ttttcttcag ccaatcccgc tttcagaacg gctgctttaa tttcctcttt cagcacgtcc
     9241 ttcatttgtt ccgcaatgtt catgcgtcaa gtcctccctc atacgtgatc gtcatattat
     9301 gtaaatgctg ctgttcgtct cccacatgca tatcataagc tatgctgata cggcctcttt
     9361 ctttgccgat gtcagactga atcgattttg tgcttgtagt cagctcaagc tcgccaaatg
     9421 acattttata tttggcaatg gtagaggctc ccggcacaaa ccgctgattc attttcactg
     9481 cgcctgacct catgacaaac acttcgctct cgcttacttt cacaatcgtt ttcactttgc
     9541 ccaaatcgtg ttcttcataa taagaaagat acactttatt ttgtttcaca tgataagatc
     9601 cggaggtgcg gaactcaatg acctcttctt tcccgtcatc cgcaatcaca gattttacat
     9661 ggagcgttat cggtgtctct tgtttcatgc tgacacgtcc ttttcatcat tccactttga
     9721 cttgaatagt ataatcattc gctatttaaa gttcaactgc ttctatctta ccatcattac
     9781 tcatcagttt tgaagataaa cctcataaaa agcgattaat gagaagaaga gcatttaaat
     9841 agaaattaat gccgttttga atgttacatt tttctgaaat ctatataata tttttcacat
     9901 tttcttcaaa atatacatat tgaaaaggta ttttgatagt tttaagatta aattacatca
     9961 gagatatgct agaaaaatta aacaagggag gatttctatt atgaaaaaag ctgtcattgt
    10021 agaaaacaaa ggttgtgcaa catgttcaat cggagctgct tgtttagtgg acggacctat
    10081 tccagacttt gaaatcgcag gggcaacagg gctattcggt ttatggggat aaagcatttc
    10141 ctaaaagagg catagtaaat tactgtgcct ctttttgaaa agtattttta ctttctatct
    10201 actccttagt tatacataca tgttttctca ttatttgtat catatgtata ggagatggta
    10261 tttttgctta tagagcggat gtttccattt atcagtgaaa gtgtaagagt tcaccagctt
    10321 cctgagggcg gagtattaga aattgattat atgcgtgata atgtctcgat ctctgatttt
    10381 gaatacttgg acctcaataa aacggcttac gagctttgta tgctgatgga cgggcaaaaa
    10441 acggcagaac aaatattaga ataccaatgc gcggcgtaca atgaatcccc cgaagaccat
    10501 aaggattggt attatgaaat gctcgacatg ctgctgaaca agcaggtcat tcggcttaca
    10561 gaccagcccg aatatcggcg cattgccaca agcgggagca gcgacttccc gatgccgctg
    10621 catgcgacct ttgagctgac tcaccgctgt aatttaaaat gcgcccactg ttatttagaa
    10681 agctcgcctg aagcgctggg aacggtatct cttgaacaat tcaagaaaac agcggatatg
    10741 ctgtacgaaa aaggcgtttt aacatgtgaa ataacgggcg gagagatttt tgtccacccg
    10801 aacgccaatg aacttcttga gtatgtatta aagaaattta aaaaggttgc cgtcttaaca
    10861 aacgggacgc tgatgcgaaa agaaagcctg gagattttaa gagcatacaa gcaaaagatc
    10921 atcgtcggta tttcactgga cagtgtccat tctgaagtcc acgattcctt tagagggaga
    10981 aaaggctctt ttgcccaaac ctgtaaaacg ataaaattat tgagtgatca cggtattttt
    11041 gtcagagtcg ccatgtcggt tttcgagaaa aatatgtggg aaattcacga catggcccaa
    11101 aaggtgcgcg atcttggtgc gaaagccttc tcttacaatt gggttgatga tttcggaaga
    11161 ggcaaggata tgatccaccc gacgaaagat gccgagcagc accgcaaatt tatggagtac
    11221 gagcaaaatg tcattgatga atttaaggat ctgattccga ttatccctta tgagagaaaa
    11281 cgtgcggcga attgcggtgc aggctggaaa tcaattgtga tcagcccgtt cggagaagta
    11341 cgcccgtgcg cgctcttccc gaaggaattt tcattgggaa atatctttca tgattcatat
    11401 gaaagcatct ttgattccgc tctcgtccat aaactatgga aagcgcaggc gccgcggttc
    11461 agcgagcatt gcaagaaaga caaatgccca ttcagcggct attgcggagg ctgttactta
    11521 aaaggcttaa actctaacaa atatcaccgt aaaaatatct gttcctgggc gaaaaatgaa
    11581 caattagaag atgttgtcca gcttatttag gagggaaatg ttttgtcacc aacacaaaga
    11641 aaatggctat tgtatatcct ttcattcgtg tttgtcatcg gcgcggttgt ctatttcgtc
    11701 aaaagcgatt atttgtttac tctgatctcc attgtaattg ccattatatt cggaatgaga
    11761 gcgcggaagg ctgaatctcg atgagcatgt tggatataca cgctttatcc gtttggtacg
    11821 aacaagataa cgtcatctta gaaaacgttg atttacattt agaaaaaggc gccgtttacg
    11881 gactgcttgg agtaaatggc gccggcaaaa caacgctgat caatacgctg acaggcgtta
    11941 atcggaattt tagcgggagc ttcaagctgt gcggcattga agttaacgcc aaaacgccgc
    12001 tgaagacatt agatcagctg aaaaccaacc gctacttcgc ggcggattat ccattgcttt
    12061 ttacagagat cacggcgaag gaatacgttt ctttcgtcca ttctctttat caaaaagaat
    12121 ttcacgaaca acaatatgtc agtttagcag aggagtttca cttttctcag tacatgaatc
    12181 ggagaatctc agagctgtct ctcggcaaca ggcaaaaggt tgttctgctg acagggttat
    12241 tgctgcgggc tcccctgttt attttagatg agccgcttgt cggcttggac gtggaatcga
    12301 ttgaagtctt ttatgagaaa atgcaggagt attgcaagtc aggcggaacc attttgtttt
    12361 cttcccatct gcttgatgtg gtgaaaaggt tttgccatta tgcagctatt cttcatgata
    12421 aacagattcg caaattgatt cctattaagg atgacacaga tctgcgccgg gaattttttg
    12481 aggtgatcgg ccatgaataa tgtgtttcaa attcttaccc tgctgttcaa acagctttac
    12541 ggccggcaag ggaaaaagga tgccattcgc atcaccgccg gacttgctgt tctggctttg
    12601 tttgaaatcg gactgatccg gcaggccggc attgatgagt cggttttagg aaaaacatat
    12661 gtcacactcg cgcttctttt tatgaatgtg tatatggtat ttctttccgt ttcttcacaa
    12721 tggaaggaat cttacatgaa gctgagctgt cttttgccaa tctcttcacg gagcttttgg
    12781 cttgctcaaa gcgtggtttt gtttgtggat gcttgtttga gaagaacgtt attctttttt
    12841 attttaccgt tgtttttatt tggaaccggc acgctgtcag ggacggaaac gttattttgg
    12901 ctcggcaaat tttcgttttt cacagtctac tccattctat taggcgttct gttaagcaac
    12961 ctttttgtca aaaagaaggg atttgtgttc ctgctgcatg cggcggcatt tgcctgtatc
    13021 tgcgccagcg tggtttttat gcccgctttg tcgatcccat tatgcggggt tcacatggca
    13081 tggggcgttt tctttgattt cccctccttt ctgcaggtgc ctgatcagca gaacaagatg
    13141 cattcattca tgcggacatc ggagttttca ttttataaga gagaatggaa tcggtttatt
    13201 tcgtcaaagg cgatgttatt aaactacatt gtcatggcgg ctttcagcgg ttttttttcg
    13261 tttcagatga tgaaaaccgg catcgctgat cagcatatca tttacattgt gattaccgcc
    13321 ttgctgttaa tttgttctcc tatcgcttta ttgtattctc ttgaaaaaaa cgaccggatg
    13381 ctgttaatca cgcttccgat taaacggaga acgatgtttt gggcgaaata tcgcttttat
    13441 tccggtttgt tggcaggcgg atttattttg atcgctatga tcgtcggttt gatcagcagc
    13501 cagccgattt cattcatgac ttttttgcag tgtatcgaat tgctgctgat gggagccttt
    13561 attcggttaa catctgatga aaaaagaccc tctttcgggt ggcagacgga acagcagctc
    13621 tggagcggct tctcaaaata tcgttcctac gtattttgta ttcccctatt cttagcaact
    13681 atcgcgggca ctgttatctc cttggcgatc atcccgattg ccgggctgat cattgtctat
    13741 ttgctgcaaa aacaggaagg tggtttcttt gatacaagga gaaaaagtga acgatttgga
    13801 aatgaacctg ttaacgactg atcaattttc aacaatctcc atcgccgcga gctttctgaa
    13861 accgatcgaa atcgaggcag agcctgaaga agaaacaatc tatttttacg gtgcggcggc
    13921 ctatctaaaa caaaacatca ctgacgtctt tggatatgct gccggcagcc gctttatcta
    13981 ctcagcgaat ctattttttg accggcagct caagacatac ggaacccgct tgattcatcc
    14041 gctatatcac aaaaatctca acattgatga tgtgctggaa acgtttgttg atagatcctc
    14101 tcttccggca tctcttccgg ccgatatcgc agaaacggct aaggctgacc ttttactgaa
    14161 gatcgaaaag aagttcgcgg atcctttctc ttactcggca gcccgtctgg cagaggaaac
    14221 gttcggcagt ccgatgtacg gcacagctat gtttggccgg aaagaaaagg tacaagcgat
    14281 acagcctaaa cgctttctgg atgcatcaga ttttaaaact gatttgttgt cccagcaaaa
    14341 acagctttat gtaataggaa acgttcaaga gttacatgcc ggaggatatt ccagacacgc
    14401 accttccatg aacggtagaa gaaaacccgt caataaaaac atttatgaaa cagagacacg
    14461 cagcaccgca ggtccttccg tgatgaccct tgggtttgat tgcggagaaa tgaacggttt
    14521 ccgtgattat ataaaaatcc agctgataga cggattgctc ggaaagtacg gccattccgc
    14581 attatttcgg cattttcgcg agaaacaaat ggcggtttac catgtcatta cccgctatga
    14641 catcatgagc aatctgctgc ttgtatccgt atgtaccaat cgtgttcaag agaaagagat
    14701 tccgccaaga gtgatcgaaa cggtcatgaa ttttcatgta gatgaacagg aattagaaaa
    14761 agcgaagcaa ttcctgaaaa atgaaatcct tctgcaatta gattctccgg aaggactctt
    14821 agcctatatg ggaattctgc gtcattactc atacaaaaaa gaagacattc tagacggtat
    14881 ttcaacgatt acctgccgcg atttattaca atacgtgacc aatattaaat atattggagc
    14941 acatgtagta aggggatgag gagattggag aaagaaacct tttttcatca gcttgatgca
    15001 cgtaccgaga tcacgtacgc agacaatgga ctaaaaatat gcagagtaaa attccccaac
    15061 gcccatatga ggctgtgtaa tgtcaaagtc aatttcggca gccggtacat ccgtttgcac
    15121 tccggagaca gcgaaaaagt gctgccgtac ggcactgcgc actttttgga gcatgttctc
    15181 ttctggcata acgggcgaaa tctatacagc gatttttttg atcatggagc gcttttgaat
    15241 gcgttcacta catatacaga tacaaatttc atgtttacct ctcttcctga tcggctggag
    15301 cacacgatcc cgaaactgct tgatgcttta tggaatccct cctttgaaga gaatattgtc
    15361 gctcaagaaa aaagcgtgat taaaagcgaa attgaaacag ctcatctcaa tcaacagctt
    15421 cattatcatt atcagatgct gaatttgctt tctcccgcat cacccgctgc gatttttcct
    15481 gcgggcagaa cggaggatat cgaggcttta acgatcaatg atctaaaaga agcctatgca
    15541 acaggctacc agccccagcg tatgacgatg tttctgatcg gcggctctga agatacagaa
    15601 accattttgc cttctcatct ccggctgcgc ggccgctcag gccataccgc caagagaaaa
    15661 ttcatgcctg catgccctcc cttcttacaa aatcagagcc tgggtgatgc ggagtgctcg
    15721 ggggaaacat ggaccggatt acatataggc gcactccccg ataaaaacga tctttttaca
    15781 ttgcaaatgt attgggacat cgtctcccgc gttctttttc atatagattc gccgttgttt
    15841 caagaaatcc ggcaatcgcg cctccttaat atccaaagct tatcagcgga aagtcacgtg
    15901 tcagaagatg gcggcttttt gatccttcac agtaaagggg cacattcttc agcctatatc
    15961 gatgtagcgg caaactatgt gatgcagcag aagtgtcagg cggaagcttg gctggagtac
    16021 ggcaaggaat ctttgctgaa cgcgatcatc tacgacagtg attatgtgcg taaatgcttt
    16081 gaatgggcgg ctgaatgtga gcggtatgat tgctcttttt tcgagcttta ccggctgatt
    16141 caaaacatga ctgtacagga ttttttatat cttattgata cagtggcttc tgctaaaaaa
    16201 gcaaccattc atgtctctca tccagaggtg gttggacaat gaaaaaaagc actgtcttca
    16261 ctgtgttatt gctgcttatg gtcatggcgg cctattcatt tggctggttt caagccagcg
    16321 ctgcagccgc ggggcaatat gtacagctga ttaaaaatga ttatgtgcgg ctcggcttgc
    16381 tttcttgtgc cgcttctgtg ctgatgctgc cggcattttt gtatcttcac tatgtcaccc
    16441 gatccgtcaa aagcatgaca gcagcttttc agaagataac gcaatctcat caaagctgct
    16501 gtgattttca gcatcgcaag ctgtgcagcc gttatgccgg cgatgtgaaa tcattgcgcg
    16561 aaagctacaa gggcatccgc caaacgtacg tgatgggggc tgtattatgc caagtgattg
    16621 tgttcggtta catgtttgaa gtggtcaagt ccactccatt cagcttcaac gcgccccctg
    16681 ctgtcacgaa ggggattgcg attgttctca tcatctattt attgttttat atgaggtcat
    16741 acctgctcca gcttctccag aatgggcaaa cggctaaaaa ggctgtcgtt ctgatattag
    16801 caggagccgg gatatggtgg atgctcagct ttaccattag tgaactgctg tttctgatta
    16861 tcttggctgt catccagcag ataggttcat ttatttataa acgagtctct tatcgcagct
    16921 ccgctcctct cgacctgtga aagcatctat atcgaaatcg cagacgggcc agcgccaacc
    16981 ggaatgaccg ctacggctgt ataatcactt gtgcggtaca ctgtcacggt attggctgca
    17041 ggctgcgcaa catagatata ttgattgtcc ggcgttacag cgaggtcaga ggcgccgggg
    17101 agagaggcag ttgttataaa ggtatgctgc gctgtatcta cgacagtgag attctccgga
    17161 aatacctcaa aatagggctc aaggaaaaac gcacgagttc cgtcagcaga aaaaacaatt
    17221 gttattaaac cgtcaaacag tctgttaatt tttgaagatt cgctaaatgt tatcaaatct
    17281 atcacagaaa cgccattcgg ccttagctca ccattttcca gataagcaaa atcccctatt
    17341 aacggggttt caataagatt tggtgtttca aagttactta aactatcaat cagctgccct
    17401 gtatttatgt catatttatt aacgacccaa gatacagagt tggtcgtata gaaaaactgt
    17461 ccgttagggt ctagggcaaa atcgtccgca gcgggtgaca ccgtgaatgt attggtaatg
    17521 gtttgcgagg aaatattaat ttcataaatg gagctgcccc ctgcgagaat ataagcaaat
    17581 cctcccccag tttcaaactg gatttttctt ggggctcctg caagagggat ggtttgtgta
    17641 accgttaatg tttcgttgtt aatgatcgat acaagattcg cattcctatg gagaacatac
    17701 gtatacgctt tatccaaggt aacacccaga tcagtcggga tatcagataa agaaatggta
    17761 cttacaagag tattcacgtc agtatcaatg accgaaaccg tgctgtctcc agtgtttgca
    17821 gtaaacacgt aagatcgctg atccggcgcc ggtacaagta caatcgtccc cgtcaccgat
    17881 aaaggactgc cgtagaacgg cggaacgaca acaaggttct cgatcgtgat gtccctgaaa
    17941 tccgggcaat catccgggtt atcagaataa tagacgactt gatcgatctc tgcattgtga
    18001 atgacagaaa cagccccgtc cccttgtcca tattgatttc ttacaggtga gacactgaca
    18061 agaagcgtaa cggtcccttg cagacgcaca gcatattgaa caccgatatc ttctggttcc
    18121 ggcccagtct ggattaagca aggctcactg atacaggaca aattgtctgt actccacgta
    18181 accgctgcag ctgcttcggc agaatcaata gtgaacccct caggaatgtc aatcggcact
    18241 tgaaacagcg cttctcggct gatctcctca tcacaaaagc agaaacaatt ttcctgaaaa
    18301 gcagaaagat catttttttt cataatgtca cgtcctttta aaaatgcaca caacatgcat
    18361 ttggttaaat cgcaatgtca ctcggtgaaa cgccaagcga tatgacagtt tccacagaat
    18421 agtcatccgt gttgtaaaca atgatgcttt gctctataaa acgagttgca tacagaaatt
    18481 cgccattcgg ggtaaacgca aaatctcttg ccgattgcgg aagatttcct attacgcctg
    18541 cgccacttgg actgacggaa tatagattat gagtgaaacc atcaaacaaa tacaatcctg
    18601 atccgctctt aaacatttga tactcaccct catactccag ctcttgggtg ctactgatcg
    18661 taaacgtatc taaatcaata atcgtcaaat aatttaagaa gtgatcttgt tctaatacat
    18721 aggcataccg ctccggcggc tttgtttcta tggaatttac aataagctct cgttcaatca
    18781 ttcctgtaac ttcaccggta gataaatcca ctttttgcac taatccaaaa tcaagaacat
    18841 aggcgaacaa cccattggga tcaattgcaa tatcataccc ttcaacagga atagaccttt
    18901 ctacggcaga cgtttcaatt ctgatcacat aaatggcatt accagcgaga acatatgcga
    18961 aatcatgttc tggcataaat ttaattgttt caggcggctg atctagcgga atggatccag
    19021 taacagtcaa tgtaccatta tcgatgacag aaatgacact attgttgaaa tgcaaaacaa
    19081 acgtagaggc tttatctggc gtaatttcta tacctgttgg ctcgtatggg agagaaatgg
    19141 ttgttataac ggtatgagta ttcgtatcaa ttacggaaac cgactgatca tctggattcg
    19201 ctgtaaacgc ataaacaggc tccggctctg gttccggggc gagcacaatc gttcctgtca
    19261 ccgataaagg actgccgtaa aacggcggga cgacaacaag attttcgatc gtgatgtcac
    19321 tgaaatccgg gcaatcatcc ggttcatcag agtaatagac gacttgatca atctctacat
    19381 tgtgaatgac agaaacagcc ccctcccctt gtccatattg gtttcgcaca ggtgagacac
    19441 tgacaagaag cgtgatggtt ccctgcaggc ggactgcaaa atggatttcg atctcatcag
    19501 gcccgggtcc agtctgaatg aagcagcgtt cactgacaca ggataggctg tccgcgttcc
    19561 atgtgacatt cgctaccgct tcagcaggat caacaacaaa cccctctggc acttctatcg
    19621 gcgcttgaaa catggcttca cggctggctt cttcctcaca aaaacataaa caatcctctt
    19681 gctgtctgac ggctttttct ttaaaagaat gctcaatctt cttcacatgc tcacttcctt
    19741 ctcagaaata gctgctaagc gcagcagtaa tagagtatgg ggacaaaaag gttttgcttg
    19801 tacaaaagtt ctaagccaaa agtgtttttt ttaactgaaa acgggtaaaa agaaaaagag
    19861 ttatttacaa attatgtttt atagcaggaa tgtcaggtta tataaaataa aaaagacttc
    19921 ataaggagga tttatttata caagcagcct tattacacta cttatataag ttcccaagct
    19981 tctctgggta aataaaagga ggatcataat gaaaaggaat acaaccttga tcggaatcgt
    20041 ttctttgcta ttactattaa tgctgtctgc atgcggcagc gataatcaag acaaaaaatc
    20101 agatcaaacg atgaatatgg acgacacaca tactcattct gagtccagtg agattccgga
    20161 aggcctgaca gcagcacagc acccaaatta taaagttgga agccaagcca tcatcaaggc
    20221 ggatcacatg aaaggcatga agggtgccga agcaaccatt acaggtgcct atgatacgac
    20281 ggcctatgct gtttcctata caccgacaac cggcggaaca agagtggaaa atcataaatg
    20341 ggtgattcaa gaagaaataa aaaatgccag tgatgaaaca ttaaaacccg gaacggacgt
    20401 gattatagaa gcggatcata tgaagggaat gaagggtgca acagggaaaa tcgattcagc
    20461 tgagaaaacg actgtctata cggttgatta caccccgaca acgggcggag aaaaagtgaa
    20521 aaatcacaag tgggtaacag aaagtgaact atcttcaaaa taaaggcaga atatgatgat
    20581 gaactgttaa acaaattggc cctatccaat atgtttaaca gtttattttt attgtgcttt
    20641 gatatggccg ggagagagag cgtttccact attcggtcac gcaaaaaaat ttgcggtttt
    20701 tttgcgcttc caaacaaaac aaaaatgata aaatatgaca tatatagaat gatgaaggga
    20761 ggggttttca aacgtgacag gcgtcatatc ttcttccatt ggagaaaaga ttaatgaatg
    20821 gtacatgtac atacgccgat tcagcatacc ggatgcggaa tacttacgaa gagaaatcaa
    20881 acaagagctg gctcaaatag aagaagacca ggatcttcat ttgtattatt cactgatgga
    20941 attccggcac aatctgatgc ttgagtatct agaaccgtta gaaaaaatga ggatagaaga
    21001 acagccgcga ctgtccgatt tactgcttga aatcgataaa aaacaggctc gtttaaccgg
    21061 actgcttgaa tactatttta atttcttcag aggtatgtat gagctggacc agcgggaata
    21121 tatattagcc attcaatttt ttaaaaaagc agagagcaaa ctgacttttg ttaaagataa
    21181 aattgataaa gctgaattct ttttcaaaat gtcagaagtt tattactata tgaagcaaac
    21241 ttatgtgtcc atggactatg cacgtcaagc ctttgaaata tacaatgaac aaactacata
    21301 tggtatcaga atgattcaat gccagtccct gtttggagct aactttttgg acctgaaaca
    21361 gtatgataaa gcaattgctc atttccaaaa agcttataca cttgctgagg cagaacagca
    21421 gccgcaacta atgggaagaa cgctttataa tattgccctt tgcaagcaaa atcaacacag
    21481 ctttgaatca gcgatagagt atgtaaaaaa atctattcat gtatttgaaa aatctaaaat
    21541 gcttccttca ctgccacaag cctttttcct aattacacaa atttatttta aattaggtaa
    21601 gattgataac
//
