LOCUS       NZ_JAHWJB010000002     23909 bp    DNA     linear   CON 12-SEP-2024
DEFINITION  Bacillus sp. FDAARGOS_1420 tig00000007_arrow_pilon, whole genome
            shotgun sequence.
ACCESSION   NZ_JAHWJB010000002
VERSION     NZ_JAHWJB010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. FDAARGOS_1420
  ORGANISM  Bacillus sp. FDAARGOS_1420
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 3674589)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3674589)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3674589)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
REFERENCE   4  (bases 1 to 3674589)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1
            Genome Coverage                   :: 1241.19x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019344915.1-RS_2024_09_12
            Annotation Date                   :: 09/12/2024 05:20:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,050
            CDSs (total)                      :: 6,900
            Genes (coding)                    :: 6,426
            CDSs (with protein)               :: 6,426
            Genes (RNA)                       :: 150
            rRNAs                             :: 12, 12, 12 (5S, 16S, 23S)
            complete rRNAs                    :: 12, 12, 12 (5S, 16S, 23S)
            tRNAs                             :: 109
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 199 of 474
            Pseudo Genes (incomplete)         :: 296 of 474
            Pseudo Genes (internal stop)      :: 116 of 474
            Pseudo Genes (multiple problems)  :: 119 of 474
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:39:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2710017
            Orig. end                         :: 2733926
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHWJB010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..23909
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="8"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..23909
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..23909
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:13909](-)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..13909)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            complement(561..1499)
                     /locus_tag="I6L50_RS24095"
                     /old_locus_tag="I6L50_24080"
     CDS             complement(561..1499)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913375.1"
                     /locus_tag="I6L50_RS24095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24080"
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="WP_002137777.1"
                     /transl_table=11
                     /translation="MLKKVFAMSTTVVLAAGLLSGCGTKESSASKNEDTKKGYVPKTLN
                     VQFVPSQNADTLEAKAKPLEKLLSDKLNIPVKVSISTNYNTIVEAMASKQVDVGFLPPT
                     AYVLAHEKKAANVILQAQRFGVDDETGAPTKDLVDVYKSEFVVKKDSNINSVKDLKGKK
                     IGYQDVTSSAGYVWPAAVLLKEGIDPLKDVKPVTLKGHDQSLIALLNGDVDAAVVFQDA
                     RNIVKKDYPNIFDQTKIVKFTEKIPNDTISVRSDLDAEWSKKLQDAFIEIGKNEEGHKI
                     IKEVYSHEGYVKSDDSKFDIVREYGKKVKTQ"
     misc_feature    1520..1536
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: medium, score: 28.44"
                     /tool="antismash"
     gene            1685..3268
                     /locus_tag="I6L50_RS24100"
                     /old_locus_tag="I6L50_24085"
     CDS             1685..3268
                     /GO_function="GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1283: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002199635.1"
                     /locus_tag="I6L50_RS24100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24085"
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase"
                     /protein_id="WP_113936923.1"
                     /transl_table=11
                     /translation="MNQNQIVTLNILLTSDVHGTVYPIHYQDNSKAEIGLAKISTLIKK
                     ERSQNANVLLIDNGDFIQGTPFTYHYATYEKDMENPMSLLANYLEYDIAIIGNHEFNYG
                     MDILNNAVATANSPYLSANILDKNRKEPYFGKPYIIKHIESNIKIAILGVTTHYIPHWE
                     QAHHIKDLQFEDAFETTKKWVSYIRKHEKPDLLVVAYHGGFERDLQTGVPTEVLTGENQ
                     GYAMCHEIEGIDILLTGHQHREIAHTVINGVTILQTGCNGHSVGKVTVTFEKAEQKWVK
                     KESYSELLSVQGIAADQDVLSLVANYEEKTQNWLDKPIGIIYGDMQISDAMQTRLQDHP
                     FIEFINKIQMDIANVSISCTSLFHNTSPGFPKHVTMRDIVSNYIYPNTLKVIRITGADI
                     KDALELSASYFTLKADGTIIVNPAYIEPKPQHFNYDMWEGISYVLNISKPIGERVESLQ
                     YKGAPLNMNEEYDVVMNNYRASGGGNFFMFQNKPVVKDIPTDMSELIANYILKHKRIEA
                     TKNENWKVIK"
     gene            complement(3309..3548)
                     /locus_tag="I6L50_RS24105"
                     /old_locus_tag="I6L50_24090"
     CDS             complement(3309..3548)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002172959.1"
                     /locus_tag="I6L50_RS24105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24090"
                     /product="DUF896 domain-containing protein"
                     /protein_id="WP_113936922.1"
                     /transl_table=11
                     /translation="MPSQELIERINFLAKKAKAEGLTEEEQRERKSLREQYLKGFRQNM
                     LNELKGIKVVNEEGTDVTPAKLKALKKQDNAKLN"
     gene            complement(3594..4250)
                     /locus_tag="I6L50_RS24110"
                     /old_locus_tag="I6L50_24095"
     CDS             complement(3594..4250)
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016111193.1"
                     /locus_tag="I6L50_RS24110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24095"
                     /product="recombinase family protein"
                     /protein_id="WP_002014433.1"
                     /transl_table=11
                     /translation="MNAIIYARVSTTKEEQETSLLRQKDELLHLAERYQMDVTKVIEEQ
                     ASGYTIERDGILEVLDTIRDEQVDVLLIQDETRLGRGNAKIALMHCLHKEGIKVYTHTH
                     SGELQLSDSDSMVLNIIGIVEEYQRKIHNLKIKRGMQRAVEKGFRPENNLKNRHLSVGR
                     EKKEVPIEEIVRLRRNELTFEEIAATLRGFGHDVSKATVHRRYVEYTKQLLDKEE"
     misc_feature    complement(4311..4324)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 22.42"
                     /tool="antismash"
     gene            4392..5006
                     /gene="lexA"
                     /locus_tag="I6L50_RS24115"
                     /old_locus_tag="I6L50_24100"
     CDS             4392..5006
                     /EC_number="3.4.21.88"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009432 - SOS response [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="lexA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413738.1"
                     /locus_tag="I6L50_RS24115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24100"
                     /product="transcriptional repressor LexA"
                     /protein_id="WP_002087894.1"
                     /transl_table=11
                     /translation="MEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHG
                     HLSRLEEKGYIRRDPTKPRAIEILGDNRTETQSVIQVPIIGKVTAGLPITAVESVEDHF
                     PLPASIVAGADQVFMLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTEDNEATV
                     KRFYKEKDHFRLQPENSSLEPIILKQVSVIGKVIGVYRDLH"
     gene            complement(5110..5913)
                     /locus_tag="I6L50_RS24120"
                     /old_locus_tag="I6L50_24105"
     CDS             complement(5110..5913)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016512438.1"
                     /locus_tag="I6L50_RS24120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24105"
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="WP_002014437.1"
                     /transl_table=11
                     /translation="MMKKILLVAIACIIIGVIGISQTYEKAVQTAEKGDKEDVIKNETL
                     KNVEVKLDAGDIVVQKSPDSSFYVKQKGDVSKQEIRIAEDGDTLKIIGEIEKGVSFDFS
                     FYNFGFQTPTLTILVPERSYQEIKVNSSAGQIEISNVKSDRVSVETLAGEVDLKHVTAK
                     SVEGSTKAGEVNFKKVIGKVTAKTASGDIDIIDHDSKYDLEVSSTAGDVDITLLEKPQD
                     AVITGQTAAGDVTIFNEDNKNITIGNGSKKISGKTAAGDVTIETR"
     gene            complement(5914..6456)
                     /locus_tag="I6L50_RS24125"
                     /old_locus_tag="I6L50_24110"
     CDS             complement(5914..6456)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031379.1"
                     /locus_tag="I6L50_RS24125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24110"
                     /product="DUF1700 domain-containing protein"
                     /protein_id="WP_219303330.1"
                     /transl_table=11
                     /translation="MSKERFLKELSSHLRKLPEEERQDILFDYEEHFQFGMEEGKTESE
                     IIKGLGSPKVIAKDLLALYRFDEMKKDPSTSNITRAVMAAVGLSLFNFIIVLGPLVAII
                     GFIFSFWVGGIASVVTPFFVIVKVFMGTFIWLDLFVSITFVGVGLLLCIVAYYSTKWFK
                     RLCVRYVVWNFKMIKGE"
     gene            complement(6449..6772)
                     /locus_tag="I6L50_RS24130"
                     /old_locus_tag="I6L50_24115"
     CDS             complement(6449..6772)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016105867.1"
                     /locus_tag="I6L50_RS24130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24115"
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_002185126.1"
                     /transl_table=11
                     /translation="MNVQFKKGVLELCVLALVKRKDSYGYELVQQISNKFLISEGSVYP
                     LLRRLTKEGYFQTYLKESTEGPPRKYYQLTEQGEMQLTLLVTEWRDFAKGVQEIIEGVE
                     WDE"
     gene            complement(7001..8800)
                     /locus_tag="I6L50_RS24135"
                     /old_locus_tag="I6L50_24120"
     CDS             complement(7001..8800)
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016096316.1"
                     /locus_tag="I6L50_RS24135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24120"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_219303331.1"
                     /transl_table=11
                     /translation="MNHIIYFMKKLYSSAGKILYVNLFGMVLISLLEGIGIILLIPMIS
                     MTGIINVGEETAFIAPVYRFLQDFPKTTSLMSILGIYILIVLGQNLLQRNITLRDARTQ
                     QAFVRELRIETYSMILKAKWSFFLEKRKTNLINILTTELARVSYGVNLILQLLAAILFT
                     FIQVGIAFLLSPQITIFVLVFGLLFLVASRVFIKKARILGGKTSKLAQGYLSGITDHFN
                     GIKDIKSNTLEASRLDWLQSITGKMSNEQMEYMRIRSNSQLFYKVSMAILIAFFILLSV
                     SIFKAQPAQLLLIILIFSRIWPRFMTIQSNLEQLAASIPAFKSLWELQEECKEAIEIQD
                     IHQHKQIKPICIEQGIECKDVHFRYNKQESLYALQNINVQIPINSMTAIVGHSGAGKST
                     LIDVLMGLIQPEKGQVLIDGTPLTTDNLLSLRRAISYVPQDPFLFNASIRDNLLMIEPN
                     ANEEEIWDALSFAAAAEFVQTLPNGLETLIGDRGVRLSGGERQRLVLARAILRRPSILV
                     LDEATSALDTENEVKIQSAIERLKGTMTIIVIAHRLSTIRNADQVIVLGKGEIVQKGEF
                     NQLAKERKGVFRSLLGSQMEVGS"
     gene            complement(8812..9999)
                     /locus_tag="I6L50_RS24140"
                     /old_locus_tag="I6L50_24125"
     CDS             complement(8812..9999)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002014444.1"
                     /locus_tag="I6L50_RS24140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24125"
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_219303332.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 3.8e-67, bitscore:
                     224.2, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRNDFDLDVEFMSKELKFLFAILKLQDDETLQTYSSEWFADIDWE
                     VFLEQAMHHRVYPVIYKKLKQVDEERIPSHVVQTLNRKYKNNTFQMLYLSAEMERINKL
                     FNEKEIRTMFLKGPVLSQDLYGDISLRTSCDLDVLIPIQNLEKVEKLLLEQGYVKDDYI
                     QTILSDWKWRHHHTTFFHPTRRIKLEIHWRLNPGPGKEPGFEELWKRKRKSTLTSNSVY
                     MLGKEDLFLFLVSHGARHGWSRLRWLVDIKKIVDQDLNWNEIHKLMRKYQMLHLGGQAL
                     ILASQLLGTRLNERMTELVAKKHSKQLAQEALFYFKKMINLHSEPVPEEVLAYHSRHLF
                     SLMSNQQKIIFILSQLYPYPIDAKTLPLPKSLHFLYFPLRPFLCLWRKTKELALS"
     gene            complement(10001..10474)
                     /locus_tag="I6L50_RS24145"
                     /old_locus_tag="I6L50_24130"
     CDS             complement(10001..10474)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012261438.1"
                     /locus_tag="I6L50_RS24145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24130"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_219303333.1"
                     /sec_met_domain="PF13471 (E-value: 2.7e-23, bitscore: 80.0,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIVKRLRVFLSLNTQTKLLFLEAFIFLGWARVLKSITFSKVAPS
                     LGDYMNETPVAQVPPHGDTLKKVSEAISIMSRYTFWESQCLVKAIAGMKMLEKRHIEST
                     IYLGTAKDNNGELIAHAWLRSGSFYVTGLEGMEKFTVVGSFAKRLSEDTIKGE"
     gene            complement(10511..10819)
                     /locus_tag="I6L50_RS24150"
                     /old_locus_tag="I6L50_24135"
     CDS             complement(10511..10819)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002128586.1"
                     /locus_tag="I6L50_RS24150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24135"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_219303334.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1.2e-25,
                     bitscore: 87.1, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.9e-15, bitscore: 54.7,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVHSRKISLEHNVVQSEGNIVSDMDGEKVMLNVQKGNYYNLGEI
                     GGEIWELIEKPIIINQLVTMLVSKYDIERKGCEEQVLSFLECLFQEGLIQIKDHPNK"
     aSDomain        complement(10532..10780)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_I6L50_RS24150_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="9.20E-28"
                     /identifier="RREFam002.1"
                     /label="I6L50_RS24150"
                     /locus_tag="I6L50_RS24150"
                     /protein_end="96"
                     /protein_start="13"
                     /score="87.1"
                     /tool="antismash"
                     /translation="VVQSEGNIVSDMDGEKVMLNVQKGNYYNLGEIGGEIWELIEKPII
                     INQLVTMLVSKYDIERKGCEEQVLSFLECLFQEGLIQI"
     gene            complement(10782..11753)
                     /locus_tag="I6L50_RS24155"
                     /old_locus_tag="I6L50_24140"
     CDS             complement(10782..11753)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108921.1"
                     /locus_tag="I6L50_RS24155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24140"
                     /product="aldolase"
                     /protein_id="WP_219303335.1"
                     /transl_table=11
                     /translation="MFNSMGNVLYKAFGLKIVSEIPLPELSQLNEKKEMADVQIEITDL
                     SEQWGKYSEEQQKTIVVEDDVVMFHVPQTAIFSVQSGEKITFSPMPGAEENKIRLYILG
                     TCMGALLMQRKVLPLHGSVIAIDGKAYALIGDSGAGKSTLASAFLSKGYQILSDDIVAV
                     SLLCKNTPIVDPSYPQQKLWKESLDEFGMEKEQFSPLFERETKYAVPVNSQFFPDPLPL
                     AGVIELVKTQGEDIEMTRIEGLARLCKLYQHTFRNSLISKLGLMEWHFETSSNIASGME
                     MFQLRRPASRFTAHELVQVILNTIQKGSMADGCSFEKNIIRT"
     gene            complement(11831..11956)
                     /locus_tag="I6L50_RS24160"
                     /old_locus_tag="I6L50_24145"
     CDS             complement(11831..11956)
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002172970.1"
                     /locus_tag="I6L50_RS24160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24145"
                     /product="paeninodin family lasso peptide"
                     /protein_id="WP_002146236.1"
                     /transl_table=11
                     /translation="MKKEWKQPVLEVLNINMTMAGPGHKYPDAAQPDPDDPVKYS"
     CDS_motif       complement(11897..11956)
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MKKEWKQPVLEVLNINMTMA"
                     /locus_tag="I6L50_RS24160_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       complement(11831..11896)
                     /prepeptide="core"
                     /locus_tag="I6L50_RS24160_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="-15.80"
                     /molecular_weight="2236.3"
                     /monoisotopic_mass="2235.0"
                     /core_sequence="GPGHKYPDAAQPDPDDPVKYS"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MKKEWKQPVLEVLNINMTMA"
                     /leader_location="[11896:11956](-)"
     gene            complement(11972..13909)
                     /locus_tag="I6L50_RS24165"
                     /old_locus_tag="I6L50_24150"
     CDS             complement(11972..13909)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274520.1"
                     /locus_tag="I6L50_RS24165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24150"
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_219303336.1"
                     /sec_met_domain="GATase_7 (E-value: 2.4e-20, bitscore:
                     70.5, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 1.7e-24, bitscore:
                     84.9, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAITGIIHFNEETISIQHGTQLMSYLQKYPADDIQIWHKENVFL
                     GCHAQWITPESVGEQLPFYNYEKQLAITADAIIDNRNELFEKLQVDYADRKNMTDSELI
                     LLSYQKWGEATPKYLVGDFAFMIWDEKKQTLFGARDFSGSRTLYFYRGEEKFAFCTIIN
                     PLFTLPYVEKKLNEQWIAEFLAIPVNFESVDSQQTVYKYIEQVPPSHTISVKNGKVNFS
                     RYYTPTTGKMLNLKSNEEYEEAFRDVYQSAVKARLRTHHKVGAHLSGGLDSGSVVSFAA
                     KDLRAENKKLHTFSYVPVEGFVDWTHKGRIADERPFIQSTVKHVGNIQDYYLELPERSP
                     LSEIDDWLETMEMPYKFFENTFWLSGVYEKASQHGIGVLLSGQRGNWTVSWGPILDYQA
                     MLLKKLHWMRFYRELYLYSRNLGVKKSRVFEVVRRKAFPFLHQLLSSEEQDVFPRIINP
                     EFAKKMNVFDVLKEQDIDITGTSISNAYDMKREQFEKTYYWSINGTYETKLSLRYALWD
                     RDPTNDLRIIQFCLSVPEEQYVQNGLDRSLIRRATKNLLPDKVRLNQRVRGVQGADGVY
                     RMAPFWNEFIEEVEELSVDPIISEFLNVEVIKKAISKICKEPRPEYAFDLDFRVLMRSL
                     IFYRFIKKSI"
     gene            complement(14189..15340)
                     /locus_tag="I6L50_RS24170"
                     /old_locus_tag="I6L50_24155"
     CDS             complement(14189..15340)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (41-167). E-value:
                     7.1e-09. Score: 27.2. Matches aSDomain:
                     nrpspksdomains_I6L50_RS24170_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002087912.1"
                     /locus_tag="I6L50_RS24170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24155"
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="WP_219303337.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 2.5e-100,
                     bitscore: 334.0, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4.5e-07, bitscore:
                     27.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQTIQHKKRIYLSSPHMGGNEQKYIQDAFDSNWIAPLGPNVDEFE
                     RELASFVGVKGGTAVSSGTAAIHLALRLLDVQEGDTVFCSSFTFVASANPIVYLGAEPV
                     FVDSEPETWNMSPQALAHALYDANKAGELPKAVILVHLYGQSAKLDEILSLCNQYDIPI
                     IEDAAESLGSTYKGKASGTFGRFGVYSFNGNKIITTSGGGMLVSDDAEALVKAKFLATQ
                     AKDPAPHYEHSEIGYNYRMSNILAGVGRGQLEVLEDRVRARRRIFNRYYEELSHIPGFY
                     FMPELENTRSNRWLTTMMIDEKESGISIETLLTTLADENIEARPTWKPLHMQPLFKESK
                     YYSHQKDNDVSARLFEKGICLPSGSNMMDEDQWRVIQAIINII"
     aSDomain        complement(14840..15217)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_I6L50_RS24170_Aminotran_1_2.1"
                     /evalue="7.10E-09"
                     /label="I6L50_RS24170_Aminotran_1_2.1"
                     /locus_tag="I6L50_RS24170"
                     /protein_end="167"
                     /protein_start="41"
                     /score="27.2"
                     /tool="antismash"
                     /translation="DEFERELASFVGVKGGTAVSSGTAAIHLALRLLDVQEGDTVFCSS
                     FTFVASANPIVYLGAEPVFVDSEPETWNMSPQALAHALYDANKAGELPKAVILVHLYGQ
                     SAKLDEILSLCNQYDIPIIEDA"
     gene            complement(15372..16067)
                     /locus_tag="I6L50_RS24175"
                     /old_locus_tag="I6L50_24160"
     CDS             complement(15372..16067)
                     /EC_number="3.1.3.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016086741.1"
                     /locus_tag="I6L50_RS24175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24160"
                     /product="HAD family hydrolase"
                     /protein_id="WP_219303338.1"
                     /sec_met_domain="HAD_2 (E-value: 2.1e-24, bitscore: 84.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVIKAIVFDMDDTLYKEKDYIVSGFKAVDEWIEENYKKTGFYNAA
                     IQLFDFGERGFVFNKTLEKLNIPYDENLISNLIEQYRFHKPDIQLLDEAEWVLNNLVNT
                     VKIGLISDGYLVTQEKKINALKLKGRFHSIILTDKLGKEYWKPSQIPYEKISKELQVPH
                     QQCVYIGDNLSKDFITAKKLNWITIHINREDGIYRNLIVEQDYKAHYTINDLRRLPDIP
                     VLKHMFLDT"
     gene            complement(16069..17046)
                     /locus_tag="I6L50_RS24180"
                     /old_locus_tag="I6L50_24165"
     CDS             complement(16069..17046)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002165241.1"
                     /locus_tag="I6L50_RS24180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24165"
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_219303339.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 6.4e-25, bitscore:
                     85.9, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGGNNMNILFTSSGRRVALIEKFKEALKQEGIKGKIFTADLKETA
                     PTIYFSDKHFVVPRVNEEGYIDELLKICRFEEIKLIIPLIDTELILLANNAKVFESIGV
                     KILVSSLELNKIANNKKYTYNFFVSNNIPTPRVYSDEEIERKEYQFPLLIKPYDGSSSK
                     GVTKIMNEKELEFFKEYIPNAMIQEYVSGEEYTIDIMVDFYGNIKTIVPRLRIETRAGE
                     VSKGMTKKDNEIIKAAESVIKALPNPVGCITLQCFKKEDGQITFIEINPRFGGGIPLSI
                     EAGANFPLWTIEMCQGEMFTERDFNWRENLTMLRYDEAVFTEST"
     gene            complement(17033..17638)
                     /locus_tag="I6L50_RS24185"
                     /old_locus_tag="I6L50_24170"
     CDS             complement(17033..17638)
                     /EC_number="2.7.8.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bac_transf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1146: sugar transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002070010.1"
                     /locus_tag="I6L50_RS24185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24170"
                     /product="sugar transferase"
                     /protein_id="WP_219303340.1"
                     /sec_met_domain="Bac_transf (E-value: 1.1e-66, bitscore:
                     221.5, seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRCFDMFISLFLLLCFSFVIALIAIFVKFKLGSPIFFKQERPGL
                     YGKPFNLYKFRTMTNEKDKDGNLLSDEIRLTSFGNFLRKYSLDELPQLINVVKGHLSLV
                     GPRPLLMAYIPLYSQEQAKRHNVRPGITGWAQINGRNSISWDEKFKLDIWYVENKSWYL
                     DLKILFLTFVKVLKSDGIQNSNHATMPVFKGSINHGGE"
     gene            complement(17756..20248)
                     /locus_tag="I6L50_RS24190"
                     /old_locus_tag="I6L50_24175"
     CDS             complement(17756..20248)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001989486.1"
                     /locus_tag="I6L50_RS24190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24175"
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="WP_219303341.1"
                     /transl_table=11
                     /translation="MVIYMVELNRWGIKNDATSPLQTTQGINNAFVWARDNGFQTVQLP
                     EGKYLIDKNNAINFFDNTTYRLYKCIFIKESNNLPKYNILLCDGIKNVNIEGAIIKGDR
                     ETHDYSSGGTHEWGHGIECRNSCYNIIIKDCEISECTGDGVITSMDFSALGGMQHPAHF
                     AQGDLNSKGELDSTKSDYTTVTKFFDVTGSLVKSVGYFYYSGDGYGGYGTGSNLNKTII
                     KVHFYAEDGSYLGYRNTRTYEFVYLNSLPANTKKVRFSFLQNYALMNGNLHYVLCAKIP
                     QYINISNSKLYENRRLGASVNGGRFVTFDSCEIFNNSNPMSRSVGCNPGYGIDVEDGYM
                     TNQKITVRNTHVYDNRAGAFICISTRGVFLENNKFKGIVSFGGSGDDYASLNNVYYGPI
                     SGRSITSGLEADGTFCSFTNDSIFGQMCNIAGGNTTLDGCVFSKSTISLSGETIKINNC
                     KFTFDDPDVNGVLDFRSKNIEIYNSLFDIRRAKGIATTGYAQTENAIFSNVKFLTTESS
                     GGHYVGAKRLILEKCEFIHKGETPNYSRMMAAEFMRVENNIFINQSFRFDGGDIYGNEN
                     LAKDTGYPTHSFRNNRIVWEKPYSLAVHEARGPGVSFIYIPRLEVMNNNIEVNGKVTSL
                     GSQYTLRVFTENYLNVSNNNIVTNNEIGINTMGTIVIEGAYRKKDSVLPTPRTTIISQG
                     NNSVNSNIIFTNNVNSQLEKNIIGNIPLSSTAAVEPNYGKYIRGEVVFNATPIIGGYIG
                     WVCISAGTANGNKWTAGKEYSVNSLVNASGKVYKAITAGISGANAPSHNSGIGTDGKLE
                     WQYVDMLAVFKRFGLIEG"
     gene            complement(20681..22219)
                     /locus_tag="I6L50_RS24195"
                     /old_locus_tag="I6L50_24180"
     CDS             complement(20681..22219)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842012.1"
                     /locus_tag="I6L50_RS24195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24180"
                     /product="oligosaccharide flippase family protein"
                     /protein_id="WP_219303342.1"
                     /sec_met_domain="Polysacc_synt (E-value: 4.2e-18, bitscore:
                     63.6, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNQLKAGALLSYVALFINSVMSIIYTPVMLNFLGQSEYGVYSLA
                     SSVAGYVGILNFGLGNAVIRYTAKYRALKDDEGISNLYGMFCVMYGVLGILALIAGTIL
                     TINADSMFSHSLSMDEVHTLTILMWIMIVNISTGIGFGFFSVVMLAYERFVFQKVIGIV
                     YAIINPLIMVPFLIKGYGSITMVIVTTLLNLMVILINIYYCFRVLKIKILFKKVERGLL
                     KEIIVFSSFIFLNLMIDKIYWSTDQVILGIFSGAAAISVYSIGASFTGYFSGFSTAISN
                     VFLSRVTSMVTKDVTDKEISNLFIRIGRIQYIILAFVFSGFIVFGREFIHLWVGKEYND
                     AFIIAIIILAPMIVSLMQSIGGIILQAKNIQTFKSIVYAITAVINVLISIVFVKWWGAI
                     GGAVGTAVAFTLGNIVIMNIYYWKKVNIDIPRFWNNIMRMSSTVVISVVFGAIMNHFFL
                     EYSWGLLIIKISIFSVVYIYLLWLFGTNEYEKDLFMKPAKNIISKCRKRMYRVH"
     gene            complement(22381..23586)
                     /locus_tag="I6L50_RS24200"
                     /old_locus_tag="I6L50_24185"
     CDS             complement(22381..23586)
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727032.1"
                     /locus_tag="I6L50_RS24200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_24185"
                     /product="glycosyltransferase"
                     /protein_id="WP_219303343.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 4.9e-31,
                     bitscore: 105.5, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKIIFMVINMNVGGTEKALLNMIAEMPKDQYDVTILMLEKYGG
                     FLGSIPREVQVKSLEGYQNIKDTLNNSPYIVFIDLLKKGKVIKAFNVIFFHLICKLMKD
                     RSLFFKYVLRDYPVIDNEYDSVVAYAGPMDFISYFVVKKIVAKKKIQWIHFDVTKVSFN
                     KVFSANIYKEFDQIFTVSNEAKNKLISIIPSIKKKTDVFPNVISSNLIQKQAKGGKGFS
                     DNFTGLRILTVGRLANEKGQDLAIRVLSRLIGEGFKVKWYCVGDGSSRKQYEELIEHYS
                     LKDTFILLGEKRNPYVYMDECDIYVQPSRHEGYCITLAEARYLNKAIVTTNFTGAKEQI
                     RHEETGLIVDVDEDDIYEAVKKLVINQNLCEYFSRNLAIENYDQGIKKKKGILKSETSN
                     CL"
     misc_feature    complement(23632..23646)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.87"
                     /tool="antismash"
     misc_feature    complement(23635..23649)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 23.18"
                     /tool="antismash"
ORIGIN
        1 tgatacacgt tgctgttgtc cccctgataa ttcatcagct cgtgcatatg ctttttctga
       61 gatattcact ctattcagcg cctggaatgc aagctccaca tcctctttcg gaaacagacc
      121 taacgttgta cgcaatgtag aatgatatcc aacccgccca gctaatacat tcttcagtac
      181 cgttgatcgc ttcacaaggt taaagctttg aaaaatcata ccaatatctc ggcgcatgcg
      241 tcgtaatcct tttccttttg cagcagtaat agactcccct tcaataatga tttctccttc
      301 tgtgatctca tgaaggcgat ttaccgatct gagaagtgta gattttccag ccccggatag
      361 cccgaccatt acaacaaact cacctttttg aatttccaag tttatattat tcaatccttt
      421 tgtaccattc ggatacactt tagaaacatt tcgaaactct atcacacatc ttacctacct
      481 tctatgtatt atcaataact gaaaagcggc aaactaactg aaatcacaca ctttccagtt
      541 agtcgccatt tccatatgta ttattgcgtt ttcactttct ttccatattc acgaacgata
      601 tcaaatttac tatcatcaga tttaacgtat ccttcatgtg agtatacttc tttaatgatt
      661 ttgtgccctt cctcattctt tcctatttca atgaaagcat cctgtaattt cttgctccac
      721 tctgcatcta aatcagaacg tacagaaatc gtatcgtttg gaattttctc cgtaaatttt
      781 actattttcg tttgatcgaa tatattcgga taatcttttt ttacaatatt gcgagcatct
      841 tggaatacaa cagctgcatc tacatcacca tttaaaagag caataagtga ctgatcatga
      901 ccttttaatg taacaggttt cacatccttt agcggatcga ttccctcttt taacaataca
      961 gccgcaggcc atacataacc cgctgatgat gttacatctt gatagccaat ttttttacct
     1021 tttaaatctt ttacactatt aatattagaa tctttcttaa caacaaactc tgatttataa
     1081 acatctacta aatcctttgt gggagctcca gtttcatcat ctaccccaaa acgttgcgcc
     1141 tgtaaaatta cgtttgcagc ctttttctca tgcgctaaca catacgctgt tggaggtaaa
     1201 aagcctacat ctacttgctt agatgccatc gcttctacaa ttgtattgta attcgttgaa
     1261 atactaactt ttaccggaat gtttaattta tcacttaata gcttttccaa tggttttgcc
     1321 ttcgcctcta acgtatctgc attttgagaa ggaacaaatt gaacatttag cgtcttcggt
     1381 acataacctt ttttcgtatc ttcattttta cttgcacttg attccttcgt tccacatcca
     1441 cttaataatc ccgctgctag tacaactgtt gtgctcattg caaaaacttt tttcaacatg
     1501 ttaatgcctc catttttcgt tgttataaaa taaccttcca taaacatgaa gaaatatatc
     1561 acatattttt acaaataatt gcttatttac agaatcttta cacattctat acattcacat
     1621 taaaattctt ttcatatatt tgatatgatt aattgaaaga taactataga aaaggtgaat
     1681 ggtgttgaat caaaatcaaa ttgtaacttt aaatatttta ttaacaagcg atgtacatgg
     1741 tactgtgtat ccaattcact accaagataa ctccaaagca gaaattggtt tagctaaaat
     1801 ttctactctc ataaaaaaag aacgttctca aaatgcaaac gttcttctga ttgataacgg
     1861 ggacttcatt caaggaacac catttactta tcattacgct acatacgaaa aagatatgga
     1921 aaacccaatg tccttattag ccaactactt agagtatgac attgcaatta tcggaaatca
     1981 tgaatttaat tatggaatgg atatattaaa taatgctgtg gctactgcaa attcccctta
     2041 tctatcagca aacattttag ataaaaacag aaaagaacct tatttcggga aaccatatat
     2101 aataaaacat attgaatcaa atataaaaat tgctatttta ggggtgacaa cgcattacat
     2161 cccccattgg gaacaagcac atcatattaa agatttacaa tttgaagatg catttgaaac
     2221 aacaaaaaaa tgggtttctt acatccgtaa acatgaaaaa ccagatttac tagtcgtagc
     2281 ctatcacggg ggatttgaac gtgatttaca aaccggagta ccgactgaag ttttaacagg
     2341 tgaaaaccaa ggatatgcaa tgtgccatga aattgaaggc atagatattt tactaacagg
     2401 ccatcaacac cgagaaatcg ctcacaccgt gattaatggt gtaacaattt tacagactgg
     2461 atgcaatgga cactcagttg gaaaagtaac agtaacattc gaaaaagcag aacaaaaatg
     2521 ggtgaaaaaa gagagttatt cagagttatt gtccgtacaa ggaattgcgg cagaccaaga
     2581 tgttctttcc ttggttgcca attatgaaga gaaaacacaa aattggcttg ataaacctat
     2641 tggaataatt tatggggata tgcaaatttc cgatgctatg caaacacgtt tgcaagacca
     2701 tccttttatt gaattcataa ataaaataca gatggatata gctaatgttt caatttcttg
     2761 tacaagtttg tttcataata catctcctgg tttcccgaaa catgtaacaa tgcgtgacat
     2821 cgtttctaat tatatttatc caaatacatt aaaagtaatt agaataaccg gagcggatat
     2881 aaaagatgct ctcgaactct ctgcttctta tttcacatta aaagcagatg gaaccatcat
     2941 cgtaaaccct gcctatatag agccaaaacc acaacacttt aattacgata tgtgggaagg
     3001 gatttcgtat gtattaaaca tttcaaaacc aattggagag agagtagagt ctttgcagta
     3061 taaaggcgct cctcttaata tgaatgaaga atatgacgtt gttatgaata attatcgtgc
     3121 aagcggtggc gggaacttct ttatgtttca aaataaacca gtagtaaaag acataccaac
     3181 agatatgtct gaacttatcg ctaactatat attgaagcat aaaaggatag aagcaacgaa
     3241 aaatgaaaac tggaaagtta ttaaataaca attttaacaa ataaaaaaag atgggctatg
     3301 cccatctttt aatttaattt tgcattatcc tgcttcttta aagcttttaa tttcgccggt
     3361 gtaacgtccg tgccttcttc gttaacgact ttaattcctt ttaattcgtt taacatattt
     3421 tgacgaaatc cttttaaata ttgttcacga agcgatttgc gctcacgttg ttcttcttca
     3481 gttaagcctt cagcttttgc ttttttcgct aagaagttaa tgcgttcaat gagttcttga
     3541 cttggcatct cgaatccccc ttctactcaa aactgattat tatcatacaa tagttattcc
     3601 tctttatcaa gcaattgctt tgtatattct acataccttc gatgtacagt tgcttttgaa
     3661 acatcatgac caaaaccacg aagtgttgct gcaatttctt caaaagttaa ttcattccgg
     3721 cgcaaacgta caatttcctc aattggcact tcttttttct cacgtcctac gcttaaatga
     3781 cgatttttca aattattttc tggacgaaat cctttctcca ctgctctttg catgccacgt
     3841 ttaattttta aattgtggat ctttctttga tactcttcaa cgatcccaat tatatttagc
     3901 accatcgaat cagaatctga aagctgtaat tcaccactat gcgtgtgcgt ataaactttt
     3961 atcccctctt tatgtaaaca atgcattaat gcaatctttg catttcctct accaagacgc
     4021 gtttcatctt gtataagtag tacatcaact tgttcatctc gaattgtatc tagcacttct
     4081 aatatgccat cacgttcaat tgtataaccg ctagcctgct cctcaattac ctttgtaaca
     4141 tccatttgat agcgctcagc taaatgcaat aattcatctt tttgacgcaa taatgatgtt
     4201 tcctgttctt cttttgttgt gctcacacgt gcataaataa ttgcattcat tttgaaccct
     4261 tctttctcac tatattttcc ttatagtgta tcataaattt gcctttcgtt cgaacttatg
     4321 tttgtagaat gttttttcgc atgttatact taataataag aaaaatgaaa gaaaacgagg
     4381 tgttagaaaa catggaaaag ttaacgaaac gccagcaaga cattctcgac tttattaagc
     4441 taaaagtaca agaaaaagga tatccacctt ccgtacgtga aatcggtcaa gcagtcggcc
     4501 ttgcttctag ttctacagtg cacggacatt tatcacgact agaagaaaag ggatatattc
     4561 gacgcgatcc aacaaaacca cgcgcaattg aaattttagg tgacaaccga acggaaacac
     4621 aatctgttat tcaagttcca atcatcggaa aagttactgc cggtttaccg attacagcag
     4681 tcgaaagtgt agaagatcac ttcccacttc cagctagtat cgttgctgga gcagatcaag
     4741 tgtttatgtt acgcatttct ggagatagca tgattgaagc cggtatcttt gatggtgatt
     4801 tagttgttgt tcgccaacaa cagtctgcat acaacggtga aattgtagtt gctttaacag
     4861 aagataacga agcaactgtt aaacgtttct ataaagaaaa agatcatttc cgtctacaac
     4921 cggaaaactc ttcactagaa cctattattt taaaacaagt gtcagttatt ggaaaagtaa
     4981 ttggcgtata tcgtgattta cattaaaaat aataaaaaga agataattcc cttatttata
     5041 taggtaatta tcttcttttt atattttgat agatttccgt tcattcaaaa gaatgcgttt
     5101 cattaattat taacgcgttt caatagtaac atctccagca gcggttttac cgctaatctt
     5161 tttactacca ttaccaatcg ttatattttt attgtcttca ttaaaaattg tcacgtctcc
     5221 agcagcagtt tgcccagtta taactgcatc ttgtggtttt tccaataaag taatatctac
     5281 atctccagca gttgaactta cctctaaatc atatttagaa tcatgatcaa taatatctat
     5341 atccccacta gccgtttttg ctgttacttt ccctattact tttttaaaat tcacctcacc
     5401 agcttttgta gacccctcca cactctttgc tgttacatgt ttcaaatcca cttctccggc
     5461 gagtgtttca acacttacgc ggtcactttt cacattactt atttctattt gtccagcgga
     5521 tgaatttact tttatctctt gataagaacg ttcaggtact aatatagtca atgtaggcgt
     5581 ttgaaatccg aaattgtaaa atgaaaaatc aaatgaaaca cctttttcta tttcccctat
     5641 gatttttaat gtatcaccat cttcagcaat acgaatctcc tgtttactta cgtctccttt
     5701 ttgctttaca taaaaagagg aatcaggaga tttttggaca acaatatctc cagcatctaa
     5761 cttaacttct acatttttta gcgtttcgtt tttaattacg tcctctttat ctcctttttc
     5821 tgcagtttgc actgcttttt catatgtttg tgaaatccct attactccaa taatgataca
     5881 agcgatagct actaataata tctttttcat catctactcc ccttttatca ttttgaagtt
     5941 ccagactaca tagcgtacac ataatctttt aaaccacttc gtactataat aagctacaat
     6001 acataacaac aaacctactc cgacaaacgt tatactaacg aaaagatcca accatataaa
     6061 agtgcccata aatactttta caataacgaa aaatggtgtc actacactag caatcccacc
     6121 gacccaaaaa gaaaatataa aaccgataat tgcaactaac gggcctaaca caataataaa
     6181 attaaacaaa ctaagtccaa ccgctgccat gacagctctt gttatattcg aagtcgatgg
     6241 atcctttttc atctcatcaa agcgatacaa agcaagtaag tctttcgcaa ttactttcgg
     6301 agagccaagt ccctttatta tttctgattc agttttcccc tcttccatcc caaactgaaa
     6361 gtgctcttca taatcaaata aaatatcttg tcgctcttct tctggtaact ttctaagatg
     6421 acttgataat tctttaagaa accgttcttt actcatccca ttccacccct tcaataattt
     6481 cttgaacacc tttagcaaaa tcacgccact ctgttactaa taacgtaagt tgcatctccc
     6541 cttgctctgt tagttgataa tatttacggg gcggtccttc cgtcgattct tttaaatatg
     6601 tttgaaaata tccctctttc gttaaacgac gtaataacgg atatacagaa ccttccgata
     6661 ttaaaaattt attagagatt tgctgaacaa gttcataacc gtaactatct ttacgtttca
     6721 ctagtgccaa aacacatagt tctaacacac cttttttaaa ctgaacgttc aaggacaatc
     6781 cccctttctc attttagtac tgttcagtac acgacaccta ttttatccat aatataccac
     6841 ttagtattgt ttattgcaag gtactgaatt atatttattc cctaaattta gaaaacgcac
     6901 aaataaaaac acgaataaga tcctgttata actgaccatt actcgggctt ttattcgatt
     6961 cgaactgtat gaatacaaaa attcattcac cctttccctt ctaagaacca acttccattt
     7021 gtgaccctaa caaacttcga aatacacctt ttctctcttt agccaattga ttaaattcac
     7081 ctttttggac aatttcacct tttcctaaga caatgacttg atctgcattg cgtatagtag
     7141 ataaacgatg cgcaatcaca ataattgtca ttgttccttt caacctttct attgctgatt
     7201 ggattttcac ttcattttca gtatctaatg cactcgtagc ttcatctaat actaaaatcg
     7261 atggccttct taaaatagcc cgagctagta caagtcgctg acgttcacca cctgaaagcc
     7321 tcaccccacg atctccaatc agtgtttcaa gcccatttgg tagcgtttgt acaaattcag
     7381 cggcggctgc aaaacttaat gcatcccaaa tttcttcctc atttgcattc ggttcaatca
     7441 tcaataaatt atctcttatg ctcgcattaa ataagaaagg atcttgtggt acataactaa
     7501 tagcccgcct taatgacaat aaattatcag ttgtaagggg agtaccatct attaacactt
     7561 gacctttttc tggctggatt aatcccatta atacatcaat taatgtactt tttcctgcac
     7621 cagaatgccc cacaatggcc gtcatactat ttattggaat ttgtacatta atattttgaa
     7681 gtgcgtataa agattcttgc ttattataac gaaagtgaac atccttacac tcaatacctt
     7741 gttcaataca aatcggcttt atttgtttgt gctgatgtat atcctgtatt tcaatcgctt
     7801 ccttacattc ctcttgcaac tcccataatg acttaaaagc aggaatactt gcagctagtt
     7861 gttctaaatt tgattgaatc gtcataaatc ttggccatat cctcgaaaag atgagtatga
     7921 ttaatagtaa ttgggccggt tgagctttaa atatacttac tgataataat ataaaaaatg
     7981 caattaaaat agccattgaa actttataaa acaattgaga gttcgatctt attctcatat
     8041 actccatttg ttcgttactc atctttcctg ttatagattg taaccaatct aaacgtgatg
     8101 cttccaaagt attactcttt atatctttaa ttccattaaa atgatctgtt atacccgaaa
     8161 gatagccttg tgccaatttt gaggtcttac cgcctagtat tcgtgctttt tttataaaaa
     8221 cacgagaagc taccaagaat aataagccaa agaccaaaac aaatatagtt atttggggtg
     8281 ataataaaaa cgcaatcccc acctgtatga atgtaaacag aatagcagct aacaattgta
     8341 aaattaaatt aaccccataa cttacgcgag ctaattctgt agttaatata tttattagat
     8401 ttgttttcct tttctctaaa aaaaaactcc acttcgcttt tagaatcata ctatatgttt
     8461 ctattcttaa ttctcgtaca aaagcttgtt gcgttctcgc atcccttagc gttatgtttc
     8521 tttgtagtag attttgacct aacactatta gtatatatat acctaaaata gacattagac
     8581 ttgttgtttt agggaaatcc tgtaaaaatc tatagacagg tgcaataaaa gcagtctcct
     8641 cacctacatt gattattcca gtcatactta tcataggaat taataaaata atacctatcc
     8701 cttcaagtaa acttattaaa accataccaa acaaattcac atacagaatt ttccctgcag
     8761 aagaatataa tttcttcata aaatatataa tatgattcat cgatgttcct cctatgacaa
     8821 agcaagctcc tttgttttcc tccaaagaca tagaaacgga cgtaaaggaa aatataaaaa
     8881 atgaagagat ttaggtaacg gtaatgtttt tgcatcaatt ggataaggat aaagttggct
     8941 caaaataaat ataatttttt gctgattaga catcagtgaa aataaatgtc tactatgata
     9001 agcaagtact tcttcaggaa caggttcaga atgcaagttt atcattttct tgaaataaaa
     9061 cagtgcttct tgtgctagct gcttcgaatg ttttttagct actaattccg tcattctttc
     9121 atttagtcgt gtacctagca actgtgatgc caaaattaat gcttgtcctc ccaaatgaag
     9181 catttggtat tttctcatta atttatgaat ctcattccag ttaagatctt gatcgacaat
     9241 tttttttatg tctactaacc aacgtaacct ggaccaacca tgtctggcac catgagatac
     9301 aagaaataaa aataagtctt cctttcctaa catatatacc gaattactcg taagtgtact
     9361 ttttctcttt cgcttccaaa gctcttcaaa cccaggttcc ttacccggtc cgggatttaa
     9421 cctccaatga atttcaagtt tgatccgcct tgttggatgg aaaaatgttg tatggtgatg
     9481 cctccatttc caatcactta atatcgtttg aatataatca tctttcacat atccttgctc
     9541 taaaagtagt ttttctactt tttctaaatt ttgaatagga atgagaacgt ccaaatcgca
     9601 ggatgttcgc aaagaaatat ctccatataa atcctgggat aatacaggtc cttttagaaa
     9661 catcgtacga atctcttttt cattaaaaag tttatttatt cgttccattt cagcacttaa
     9721 ataaagcatt tgaaatgtat tatttttata ttttctattt aaagtttgta caacatgtga
     9781 aggaattcgt tcctcatcta cctgtttaag ttttttataa atcaccggat aaacacgatg
     9841 atgcatagct tgctcaagaa aaacctccca atcaatatct gcaaaccatt cactactgta
     9901 agtttgcagt gtttcgtcat cttgtaactt caaaattgca aaaagaaatt tcaattcctt
     9961 agacatgaac tctacatcaa gatcaaaatc attcctcatt ttattctcct ttaatagtgt
    10021 cctcacttaa cctctttgca aatgatccaa cgacagtaaa tttctccatt ccttctaatc
    10081 cagtaacata aaaagaaccg ctccgtaacc atgcatgcgc aattaattcc ccattattat
    10141 ccttagcggt tcctaaataa atagtactct caatatgacg tttttcgagc attttcatcc
    10201 cagctatagc tttaacaagg cattgacttt cccaaaaagt ataacgactc ataatagaaa
    10261 tagcttcaga caccttcttt agtgtatccc cgtgtggcgg aacttgagca actggtgttt
    10321 cgttcatgta atcccctaaa gaaggtgcaa ctttggaaaa ggtaatactt tttaatacac
    10381 gcgcccaccc taaaaaaata aaagcctcta aaaataaaag ctttgtttgt gtgttcaatg
    10441 ataaaaatac ccttaacctt ttcacaatat tcattaatcc ctccctaaca attctcttaa
    10501 agttatgaat ctacttatta ggatggtctt ttatttgaat taaaccttct tgaaacaaac
    10561 actctaaaaa ggaaagaact tgctcttcac aaccttttcg ttcaatatca tatttagata
    10621 ctaacatagt aacaagctga ttaattatta tcggcttttc aataagctcc catatttctc
    10681 cgcctatttc tcctaagtta tagtaatttc ctttttggac atttaacata actttttctc
    10741 catccatatc gcttactata ttaccttcac tctgtacaac attatgttct aatgatattt
    10801 ttctcgaatg aacaaccatc agccatactc cctttctgta tagtatttaa aataacttgc
    10861 acaagctcat gagctgtaaa tctcgaagcc gggcgtctta attgaaacat ttccattcca
    10921 cttgcaatat tagatgaagt ttcaaaatgc cattccatca atcctaattt agaaatcaaa
    10981 gaattcctaa aagtatgttg gtataatttg cacaagcgtg cgagaccttc aatacgggtc
    11041 atctcaatat cttctccctg cgtttttact aattcaataa caccagcaag aggtaatgga
    11101 tcgggaaaaa attgagaatt aacaggaaca gcatacttcg tttccctttc gaacaaaggg
    11161 ctaaattgct ctttttccat tccaaactca tctaaacttt ctttccataa tttctgttgt
    11221 gggtaagacg gatcaactat cggggtattc ttacataaaa gagacacagc aactatatca
    11281 tcacttaaaa tttgatatcc tttactcaaa aaagccgagg ctaaagttga ttttccagcc
    11341 cctgaatcac caattaaagc atatgctttc ccatctatag caattacact tccatgcaac
    11401 ggaagtactt tgcgttgcat taaaagtgcc cccatacaag tcccaagaat atatagtcgg
    11461 attttatttt cttccgcccc tggcataggt gaaaacgtaa ttttctcgcc cgattggaca
    11521 gaaaatattg cagtctgagg aacatgaaac atcactacat catcttccac tacaattgtt
    11581 ttttgctgtt cttcgctata tttaccccat tgttcagata aatccgttat ttcaatttga
    11641 acatccgcca tttctttctt ttcattcagc tgagacaatt cgggtaatgg gatttcactt
    11701 actattttta acccgaaggc tttgtataac acatttccca tcgaattgaa catcctattc
    11761 accccacatt gtcaattttt tgaaagctct tatataacaa agttatataa gagctttatt
    11821 ttccagtgac ttaactatac ttcaccggat catctggatc aggttgagca gcatcaggat
    11881 atttatgtcc aggccccgcc atagtcatat taatattaag tacttctaat acaggttgtt
    11941 tccattcctt tttcatacgt atcacctcct ttcatattga tttttttata aatctataga
    12001 agattaggct gcgcattaaa actctaaaat ctagatcaaa tgcatactct ggtcgaggtt
    12061 ctttacagat tttcgagata gctttcttaa ttacctctac atttagaaat tcagagatta
    12121 ttggatctac actaagttct tcaacttcct caatgaattc attccaaaaa ggagccatac
    12181 ggtacacacc atctgcacct tgaacacccc gaacgcgttg atttaatcgt actttatcag
    12241 gaagtaaatt ttttgtggct ctccggataa gagatcgatc taaaccattt tgaacatact
    12301 gttcttccgg gactgacaaa caaaattgaa taatccgtaa gtcatttgta ggatcacgat
    12361 cccataaagc ataacgtaac gataatttcg tttcatacgt tccatttatg ctccaataat
    12421 atgttttttc aaactgttct cttttcatgt catatgcatt tgagatagag gtacctgtta
    12481 tatcaatatc ctgttctttg agaacatcaa aaacattcat tttttttgca aattccggat
    12541 tgattatcct aggaaaaaca tcttgttcct ctgaagaaag taactgatgc aaaaatggaa
    12601 atgcctttct tcttaccact tcaaaaacac gtgatttttt cactcctaga tttctactat
    12661 ataaatatag ttcacgataa aaacgcatcc aatgtagctt ttttaacaac attgcctggt
    12721 aatctaaaat aggcccccac gatactgtcc aattacctct ttgaccacta aggagtaccc
    12781 ctattccatg ctgagatgct ttttcataaa ctccgctcaa ccaaaaagta ttctcaaaaa
    12841 atttatatgg catctccatt gtttccagcc aatcatcaat ttccgatagt gggcttctct
    12901 ccggcagttc taaataataa tcctgtatat tccctacatg ctttacagtt gattgaataa
    12961 atggtctttc gtcggctatc ctacctttgt gtgtccaatc tacaaatcct tctactggga
    13021 cataactaaa cgtatgtaat tttttatttt cagcacgtaa atcttttgca gcaaagctaa
    13081 cgacagaacc agagtctagc cctccgctca aatgagctcc tactttgtga tgtgttctta
    13141 aacgtgcctt cactgcactt tgatacacat ctcgaaatgc ctcttcatac tcttcatttg
    13201 attttaaatt aagcattttc ccagtagttg gtgtatagta tcgcgaaaaa ttaactttgc
    13261 cattctttac cgaaatagtg tgtgatggag gcacctgttc tatatattta taaactgttt
    13321 gctgtgaatc tactgattcg aaatttacag gtatagctaa aaattcagca atccactgct
    13381 cattcaattt tttctcaaca tatggtagag taaataatgg atttataata gtgcaaaaag
    13441 caaacttttc ctcaccacga taaaaataaa gggttcggct tcccgaaaaa tctctggctc
    13501 caaaaagggt ttgttttttc tcatcccaaa tcataaaggc aaaatcgcct accaaatatt
    13561 ttggagtcgc ttccccccat ttctgataag agagtaaaat caactcacta tctgtcatgt
    13621 tttttctatc tgcataatcc acttgtaatt tttcaaataa ctcattacga ttatcaataa
    13681 tagcatctgc tgtaattgcc agttgctttt cgtaattata aaagggtaat tgttcaccaa
    13741 cagattctgg tgtgatccac tgtgcgtgac atcctagaaa aacattttct ttatgccata
    13801 tttgaatatc atcagcaggg tacttttgta aataactcat taactgcgtg ccatgctgaa
    13861 tcgatatagt ttcttcatta aaatgaataa tacctgttat agcactcatt ttccccaccc
    13921 cttatacaac aagttaccgc aaaggttctt ttttatttaa tacaacttcg cccttgattt
    13981 aattcttttt aaagaacaaa attacacatt tcaactccca atcaatcaca cactctaaat
    14041 cacccataaa cctagttata tatacattta aattatcatt atattaattt ttcgtcaata
    14101 tataaatggg aatataaacc aaatatacaa cattaaggtt cattataatg aaccttaatg
    14161 tacttgtttc tctgtgaatt attccatgtt atataatgtt tataatcgcc tgaataactc
    14221 tccactgatc ctcatccatc atattggatc cagaaggaag acaaatcccc ttttcaaata
    14281 accttgctga tacatcatta tctttctgat gagaataata cttactttct ttaaaaagtg
    14341 gctgcatatg tagtggcttc cacgtaggac gtgcttcaat attctcatct gctaaagttg
    14401 tcaataatgt ttcaatagag ataccagatt ccttttcatc aatcatcata gttgtaagcc
    14461 aacgattcga gcgagtattt tctaattccg gcataaaata aaatccaggt atgtgagata
    14521 attcttcata ataccgatta aatatacgcc ttctcgccct cactctatct tctaacactt
    14581 ctaattgccc tctcccaaca cctgctaaaa tattactcat tcgataatta tatcctatct
    14641 cactatgttc ataatgtggt gctggatcct ttgcttgcgt cgctaaaaat tttgctttca
    14701 ctaatgcttc cgcatcatca gatacaagca ttccaccacc cgaagtcgta atgattttat
    14761 ttccgttaaa tgaataaacc ccaaacctcc cgaatgtacc gcttgctttc cctttatatg
    14821 tagaacctag agattctgct gcatcctcga ttataggaat atcatattga ttacaaagcg
    14881 aaagaatttc atctaactta gcactttgac catataaatg aacaagaata acagccttcg
    14941 gtaattcccc tgctttatta gcatcatata aggcatgcgc tagcgcttgt ggtgacatat
    15001 tccatgtttc cggttctgag tcaacaaata ccggttcagc ccctaaataa acaatcggat
    15061 tcgcactcgc tacaaaggtg aaacttgaac aaaacactgt atctccctct tgaacatcta
    15121 ataaccgtaa agctaaatga atggctgccg tacctgaact aactgcagtc ccacctttaa
    15181 ctcctacaaa agatgcaagt tctctttcaa attcatctac atttgggcca agaggggcaa
    15241 tccagtttga gtcaaatgca tcttgaatat atttttgttc atttccgccc atgtgcggtg
    15301 aagagagata gattcgtttt ttatgctgaa tggtttgcat gtgaacacat cctttataca
    15361 tatttttaaa attatgtatc taaaaacata tgctttaata caggtatatc aggaagtctt
    15421 cttaaatcat ttattgtata atgcgcttta taatcttgct caactataag attacggtaa
    15481 atcccatctt ctctattaat atgaattgtt atccaattta gtttcttggc agttataaaa
    15541 tccttactta aattatctcc tatataaaca cactgctgat gaggaacctg tagttcctta
    15601 cttatttttt cataaggaat ttggctcggt ttccaatatt cttttccaag cttatcagtg
    15661 agaattatcg aatgaaatct cccttttaac tttaatgcat taatcttctt ttcttgggta
    15721 actaaatatc cgtctgaaat aagtcctatt ttaacggtat taactaaatt atttagtacc
    15781 cactccgctt catccaacaa ttgaatatct ggtttatgaa acctatactg ttctatcaaa
    15841 ttactaatca aattttcatc gtaaggaatg tttaactttt caagcgtttt attaaacaca
    15901 aaccctcttt ccccaaaatc aaataattga attgcagcat tataaaatcc agttttcttg
    15961 tagttctctt caatccactc atccactgct ttaaaaccac ttacgatata atccttttct
    16021 ttatataaag tatcatccat atcaaagaca attgctttaa tcaccatgtt aagtactctc
    16081 cgtaaaaacc gcttcatcat atctaagcat cgttaagttt tctctccaat tgaaatctcg
    16141 ctccgtaaac atttcccctt gacacatttc aattgtccat agtggaaaat ttgctcccgc
    16201 ttcgatcgat aacggaatcc ctccaccaaa acgaggattt atttcaataa acgtaatttg
    16261 cccatcttcc tttttaaaac attgtaaagt aatacatcca acaggattag gaagagcttt
    16321 tattacactt tcggcagctt tgataatttc attatccttc ttggtcatcc ctttacttac
    16381 ctcacctgct cgtgtctcta tacgtaacct aggaacaatc gtttttatat tcccataaaa
    16441 atctaccatt atatctatag tatattcttc ccctgaaaca tactcttgta tcatcgcgtt
    16501 aggtatatac tccttaaaga attccaattc cttttcattc attatttttg ttacaccttt
    16561 actagaactt ccatcataag gcttaatcaa taatggaaat tgatattctt ttctttcaat
    16621 ttcttcatca ctatatactc tgggcgttgg tatattatta gaaacaaaga agttatacgt
    16681 gtattttttg ttattagcta ttttatttaa ttccaaacta gaaactagta tttttacacc
    16741 gatactttca aaaaccttcg cattattagc caaaaggatt aattctgtat caattaacgg
    16801 aattattagt tttatttctt caaatctaca tatctttaat aactcatcta tatacccttc
    16861 ttcatttaca cgtggaacaa caaaatgctt atcgctaaag taaatagtag gagcagtttc
    16921 ttttaagtct gcagtaaaga ttttcccttt gattccttct tgtttaagtg cttccttaaa
    16981 tttttcaatt aaagctactc tcctacctga gctagtaaac aaaatattca tgttattccc
    17041 ccccatggtt tatactcccc ttaaaaaccg gcatagttgc atgattacta ttctgtatcc
    17101 catctgattt taagactttc acaaaagtta aaaataaaat tttcaaatct aaataccaac
    17161 tcttattctc aacataccaa atatctagtt taaatttctc atcccatgaa atactatttc
    17221 taccattgat ttgagcccat ccagtaatac ccggtcttac attatgtctt tttgcctgtt
    17281 cctgtgaata taaaggtata tatgccatta ataatggtcg aggccccact aaacttaagt
    17341 gaccttttac aacatttatg agttgtggta attcgtctaa actatacttc cttaaaaaat
    17401 tcccgaaaga agttaatcta atttcatctg acagtaaatt tccatcttta tctttttcat
    17461 tagtcattgt acgaaattta taaagattaa atggctttcc atataaacct ggacgctctt
    17521 gtttaaagaa tattggagac cccaatttaa acttaacaaa aatggctatt aaagctataa
    17581 caaaagaaaa acacaataat aaaaatagag aaataaacat atcaaaacac cgtttcattt
    17641 attatcactc tcctaaccct ttatttgttt tcaatctttt cctcgaaagt ttcacctatt
    17701 aatcaattca tattatctac ataaaaaaca taaaaaaggt tagaataaaa ttattctaac
    17761 cttctatcaa accaaaacgt ttaaatactg ctaacatatc aacatattgc cactctagct
    17821 ttccatctgt gcctatacca ctattatgcg aaggagcatt cgcaccacta atacctgctg
    17881 ttatagcttt atacactttt ccactggcat ttactaaact atttactgaa tactctttac
    17941 ccgccgtcca cttgtttcca ttcgctgtac cagcacttat acaaacccac ccaatatatc
    18001 caccaataat tggagtagca ttaaatacta cttctccacg aatgtacttt ccataattag
    18061 gttctactgc tgctgtagat gaaagcggta tatttcctat tatattcttt tctaattgtg
    18121 aattaacatt attcgtaaag attatatttg aattaactga attattacct tgagatatta
    18181 tagttgttct tggtgtcggc aacactgaat ctttttttct gtaagctcct tcgatcacta
    18241 ttgttcccat cgtattaatt ccaatttcat tattcgtaac aatattgttg tttgaaacat
    18301 tcaaataatt ttccgtgaac acccttagcg tatattgtga accaagcgaa gtaactttgc
    18361 catttacctc tatattatta ttcataacct ctaatcttgg tatataaata aacgatacgc
    18421 ctggtcctct tgcttcatga actgctaaac tataaggttt ttcccaaaca atccgattat
    18481 ttctaaaact atgcgttgga taccccgtat ctttcgctaa attctcatta ccatatatat
    18541 ctccaccatc aaatctaaaa gattggttta taaagatatt attctcaact ctcataaatt
    18601 cggccgccat catacgacta taattaggtg tttctccttt atgaataaac tcacactttt
    18661 caagaattaa tcttttagca ccaacataat gaccaccgct tgattcagta gttaaaaatt
    18721 tcacattaga gaatatagca ttttctgttt gagcataacc agttgttgca attcctttcg
    18781 ctctcctaat atcaaataag gaattatata tttctatatt tttacttcta aaatctaaca
    18841 ctccatttac atccggatcg tcaaaagtaa atttacagtt gttaattttt atagtttctc
    18901 cacttaaaga aattgtactt tttgaaaaaa cacaaccatc caaagtcgta tttcctcctg
    18961 ctatattaca catttgtcca aatatagaat catttgtgaa cgagcaaaaa gttccatcag
    19021 cttctaatcc acttgttata gaccttcctg atattggtcc ataataaaca ttatttaaag
    19081 atgcgtaatc atccccactc cccccgaaac taacaattcc tttaaattta ttattctcta
    19141 aaaaaacacc ccgtgtactt atacatataa atgcacctgc tcgattatca taaacgtgag
    19201 tatttctaac cgtaattttt tgatttgtca tatatccatc ttcgacatct ataccgtagc
    19261 ctggattaca cccaacagaa cggctcattg gattgctatt attaaaaatt tcacaactgt
    19321 caaacgtcac aaaccttcct ccattaactg aagccccgag cctgcgattt tcataaagct
    19381 tactattact tatattaata tattgtggta ttttggcaca caatacataa tgtaaattcc
    19441 cattcatcaa tgcataattt tgtaaaaatg aaaatcttac tttttttgta ttagcaggaa
    19501 gtgaattcaa atacacaaat tcatacgttc ttgtatttct ataccctaaa tatgatccat
    19561 cttccgcata aaaatgtacc ttaataatag ttttgtttaa attactccct gttccatacc
    19621 caccgtatcc atctccagaa taataaaaat atccaacaga ttttactagg cttcctgtaa
    19681 catcaaaaaa tttggttact gttgtataat cagactttgt actgtctaat tcacctttac
    19741 tatttaaatc tccttgtgca aaatgcgcag ggtgttgcat accacctagt gctgaaaaat
    19801 ccatagatgt tataactcca tcccctgtac attcacttat ctcacaatcc ttaattatta
    19861 tgttataaca tgaatttcta cattcaatcc catgccccca ttcatgtgta ccaccacttg
    19921 aatagtcatg agtttctcta tcacctttta ttattgctcc ctctatgttc acatttttta
    19981 ttccatcaca taataatatg ttgtatttcg gaagattatt agattccttg ataaatatac
    20041 acttataaag tctatatgtt gtattatcaa aaaagtttat tgcattattt ttatctatta
    20101 aatatttccc ctcaggtaac tgaacagttt gaaagccatt atcccttgcc catacaaaag
    20161 cattatttat gccctgtgta gtttgcaaag gagaagttgc atcatttttt attccccagc
    20221 gattgagttc aaccatatat ataaccattt aattcatcta cccccattaa tatccgctta
    20281 tattgtatat aatcttctaa taactatcat attcatttag tggatctatt gtatggatag
    20341 atgtgctact tccttgacta aacctctcta acgctggaca aatggggata ttacaaacta
    20401 tgtcttgtat ttattcgcaa agtatcctat tacctcttca tctacacttc gatcaaattt
    20461 ccttgcaaaa tatatgctat tcgtggttta cttctaaaac tcttatcgta ttccaatagt
    20521 attttcgagt tctctctcat atctttgttc ataattagcc cccacaaaat caacataatg
    20581 aaccattaca ttcgatatta agaacgatta aaggggatta accattttta tttgtattta
    20641 aaaataaatc ccccctgatc tccaatcctg tacattatcc ctaatgtacc ctatacattc
    20701 gctttctaca tttagatatt atattttttg ctggcttcat aaataaatct ttttcatatt
    20761 cattcgtccc aaacaaccat agtaagtaaa tatatactac agaaaatata ctaatcttaa
    20821 taattaataa cccccaacta tattcaagaa aaaaatgatt cattatcgcc ccaaatacca
    20881 cacttattac aacagtagaa ctcatcctca taatattgtt ccaaaaccta ggtatatcta
    20941 tattaacttt tttccaataa tatatattca ttatcactat attacctaaa gtgaatgcca
    21001 ctgcagttcc tacagcacca cctatagctc cccaccattt tacaaataca atacttatca
    21061 gtacattaat tacagctgta atagcataga ctatcgattt gaatgtttga atatttttcg
    21121 cttgaagtat aatcccccct atactttgca ttaacgatac gatcatgggt gccaaaataa
    21181 taatagctat aataaaagca tcattatatt ctttaccaac ccataaatga ataaactctc
    21241 gcccaaaaac aataaatcca ctaaaaacaa atgctaatat aatatattga attctaccga
    21301 ttctaataaa taaattcgag atttctttgt ctgttacatc ttttgttacc atacttgtca
    21361 ccctacttaa aaacacattg gatattgctg ttgaaaaacc cgaaaagtat cctgtaaaag
    21421 atgctccaat tgaatatact gaaattgccg ctgctccgct aaatatccct aatataactt
    21481 gatccgtaga ccaatatatt ttatcaatca ttaaattcag aaaaataaat gatgagaata
    21541 ctattatttc ttttagtaac cctctttcaa cctttttaaa taaaatttta attttaagca
    21601 ctctgaagca atagtatatg ttaatcaaaa ttaccatcaa gtttaacagt gttgtcacta
    21661 tcaccattgt aatagaacca taacccttaa taagaaaggg taccattata aggggattta
    21721 taatggcata cactatacct atcactttct gaaaaacaaa ccgttcgtaa gctaacatga
    21781 caacactgaa aaaaccaaat cctattcctg tagatatgtt cactatcatt atccacatta
    21841 aaattgttaa cgtatgaact tcatccatgc ttaaagagtg tgaaaacatg ctatcagcat
    21901 ttatagttaa tattgtgcca gctattaatg ctaatattcc taatactcca tacatcacac
    21961 aaaacattcc atataaattc gagattcctt catcatcctt taaagctcta tattttgctg
    22021 tatatctgat tactgcattt cctaatccaa agttcaatat ccccacataa cctgccaccg
    22081 aagatgctaa actatataca ccatattctg attgacctag aaaattcaac ataactggag
    22141 tatatattat tgacataaca ctattaataa ataacgccac atatgatagt aatgcaccgg
    22201 ctttaagttg atttttcatc ttaacaaatt acctttcatc taacataatt aatagtttaa
    22261 ttaatcaaca ctatctaaca tattattgat ggttcccctc caaattgagc cctcatcttc
    22321 agcagtatta ttattttact aatccctaac ctacttttaa aaatttaatt ttatgtccct
    22381 ttataaacaa ttagaagttt ctgattttaa aatccctttc ttttttttga tgccttgatc
    22441 ataattttct atcgctaaat tccgagaaaa atattcacat aaattttgat taattaccaa
    22501 tttctttaca gcttcataaa tatcatcctc atctacatct acaattagtc ctgtttcctc
    22561 atgtcttatt tgttctttag caccagtaaa attcgttgtc acaatagctt tatttaagta
    22621 tcttgcttca gcaagtgtta tacaataccc ttcatgccta gacggctgta catatatatc
    22681 acattcatcc atataaacat atggatttct tttttctcct agtaaaataa atgtatcttt
    22741 aagcgaataa tgctctataa gttcttcata ctgctttctt gaactcccat cacctacaca
    22801 ataccattta actttgaaac cctcaccaat taatctagat aatactcgta tagctaaatc
    22861 ttgtcctttc tcgtttgcta atctaccaac tgtcaatatc ctcaacccag taaaattatc
    22921 actaaatccc tttccacctt ttgcttgttt ttgaatcaaa tttgaggaaa tcacatttgg
    22981 aaatacgtct gttttctttt ttatagaagg tataatgcta atcaacttgt ttttcgcttc
    23041 atttgatact gtaaaaattt gatcaaattc cttatatata ttcgctgaaa acactttatt
    23101 aaaagaaacc tttgttacat caaaatgaat ccattgaatt ttctttttgg ccactatctt
    23161 ctttacgaca aaataactta taaagtccat aggtccagca taggcaacta cactatcata
    23221 ttcattatca attaccggat aatcccttaa tacatactta aaaaaaagac ttctatcttt
    23281 cattaactta catattaagt gaaaaaaaat aacattaaaa gcttttataa cctttccttt
    23341 ttttagtaaa tctataaaaa ctatatacgg tgaattattt agtgtatctt ttatattttg
    23401 atatccttcc aaagatttaa cttgaacttc tctaggaatt gaacctaaaa accctccgta
    23461 tttttccaac attaaaatcg ttacatcata ctgatctttc ggcatctctg ctatcatatt
    23521 aagtaatgcc ttttcagttc cgcccacatt catatttata accataaaaa tgattttttt
    23581 tttcatattt cctccaatat agatttttat gttcctaaaa atctacttag tttttcaaaa
    23641 tgattatcag ctctaccact tataatattc cgttctaatt tataagataa tgacttattt
    23701 ataatatttt catcgtattg catatgcaaa ttatccatat ttattaaagt acccttaaac
    23761 tttatatcat taagcatatt actagttttt tgactatata ttacaggtac aaccccaaca
    23821 tctaataata accctagtat atttgcatga aatctcgctg caattaacaa tttaaaagaa
    23881 gctatcaatt cgatagcttc ttctaaatt
//
