LOCUS       NZ_JAHWJB010000002     10252 bp    DNA     linear   CON 12-SEP-2024
DEFINITION  Bacillus sp. FDAARGOS_1420 tig00000007_arrow_pilon, whole genome
            shotgun sequence.
ACCESSION   NZ_JAHWJB010000002
VERSION     NZ_JAHWJB010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. FDAARGOS_1420
  ORGANISM  Bacillus sp. FDAARGOS_1420
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 3674589)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3674589)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3674589)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
REFERENCE   4  (bases 1 to 3674589)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1
            Genome Coverage                   :: 1241.19x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019344915.1-RS_2024_09_12
            Annotation Date                   :: 09/12/2024 05:20:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,050
            CDSs (total)                      :: 6,900
            Genes (coding)                    :: 6,426
            CDSs (with protein)               :: 6,426
            Genes (RNA)                       :: 150
            rRNAs                             :: 12, 12, 12 (5S, 16S, 23S)
            complete rRNAs                    :: 12, 12, 12 (5S, 16S, 23S)
            tRNAs                             :: 109
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 199 of 474
            Pseudo Genes (incomplete)         :: 296 of 474
            Pseudo Genes (internal stop)      :: 116 of 474
            Pseudo Genes (multiple problems)  :: 119 of 474
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:39:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1768149
            Orig. end                         :: 1778401
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHWJB010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10252
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10252
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10252
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5252](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5252)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(403..1533)
                     /gene="dnaN"
                     /locus_tag="I6L50_RS19235"
                     /old_locus_tag="I6L50_19225"
     CDS             complement(403..1533)
                     /EC_number="2.7.7.7"
                     /GO_component="GO:0009360 - DNA polymerase III complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /gene="dnaN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394467.1"
                     /locus_tag="I6L50_RS19235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_19225"
                     /product="DNA polymerase III subunit beta"
                     /protein_id="WP_219302425.1"
                     /transl_table=11
                     /translation="MEFIVNHKQFTQALSEVSKAISTKTLIPILSGIKITADQSGITLI
                     ASNSNIFIEKFIPISIEDEQITTILKAGSIVVPAKYFIEIIKKMPSEILIKSMNEQLIT
                     IQSDEITLNLNGLSANEFPNVPFLDAHTEIQVETEQLIEVFKQTVFATAKNESRPVLTG
                     VHFVIDHNKLICAATDSHRLALREILISSHAKANCIVPSSTISELLKLMNNNSNLVYIY
                     LSESHIIFKFGTITLYSRLIEGKYPNISGLIPNESQTVINIDRKKILQGVDRSSLLASE
                     WANNNVNLEIIDECTIQISSNASQIGKICETQQIDAIHGEKQLNISFDGRYMVDTLKAI
                     KEDRVTLSFGGSMRPILIEAGEQSAAVYLISPVRTY"
     gene            complement(1564..2007)
                     /locus_tag="I6L50_RS19240"
                     /old_locus_tag="I6L50_19230"
     CDS             complement(1564..2007)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="I6L50_RS19240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_19230"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_219302427.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDETHEKLLMVKNKGENGSYYTLPGGAVKFGETL
                     EEAAIREVKEETGLDISVKGVCSISEAFFEERGHHAIFFNFLGEIIGGELCISRPKEIE
                     EITWMELDSAEPYLRIPEHLKSLLQKKETVPYIFNGTIIHQSS"
     gene            complement(2197..3405)
                     /locus_tag="I6L50_RS19245"
                     /old_locus_tag="I6L50_19235"
     CDS             complement(2197..3405)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="I6L50_RS19245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_19235"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_219302429.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAGKLQQKFGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYVNGNLIDSLGVS
                     QAFLYWGIIVLFLVLIGSFFLREAIVSNTTAETLHNDYTPREMMRTKQVYFLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSTATAANAVAMIAIFNTIGRIILGTLSDKIGRLKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLCVISFVISIVIRPPNAEKKKEIKSLNR
                     KIA"
     gene            complement(3684..3827)
                     /locus_tag="I6L50_RS19250"
                     /old_locus_tag="I6L50_19240"
     CDS             complement(3684..3827)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="I6L50_RS19250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_19240"
                     /product="hypothetical protein"
                     /protein_id="WP_002013225.1"
                     /transl_table=11
                     /translation="MKNEVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFEEI
                     EY"
     gene            complement(3859..4887)
                     /locus_tag="I6L50_RS19255"
                     /old_locus_tag="I6L50_19245"
     CDS             complement(3859..4887)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002085304.1"
                     /locus_tag="I6L50_RS19255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_19245"
                     /product="serine hydrolase"
                     /protein_id="WP_219302431.1"
                     /transl_table=11
                     /translation="MYKYEKLISWVENIKETNQSSTAALCIMKDNKIVLEHYSGTHSNT
                     PTSKKVTISSQFNVASARKSYLGLMVAYAVYEGKIKSLDDEAVNYFKEYDPSLLGKTTI
                     RHLVTHSHGLNETDDGTAFREFEPGQAWAYRDINVRMVTQLIYQLYNKSFPELLKERVF
                     LPANFNETGWRIEHDENLVKVIVNPNEDAISSVGAVDDGSEKNLFVSAREFAYWGNLHL
                     NKGFLNGKQIVPKEVIALATSLQSPEYTNKELPQNGLFWFVQNEPALFSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSSSKNNMV
                     RV"
     gene            complement(5001..5252)
                     /locus_tag="I6L50_RS19260"
                     /old_locus_tag="I6L50_19250"
     CDS             complement(5001..5252)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001869.1"
                     /locus_tag="I6L50_RS19260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_19250"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_219303726.1"
                     /sec_met_domain="TIGR03601 (E-value: 1.4e-42, bitscore:
                     141.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEFQQELQALNLNAYQPGNVVYWDQQQNQYPYYYIQDDARRCGG
                     RCGGCGGRCGGCGGGRCGGCVGCVGCFSCSNCWNWWFI"
     misc_feature    5266..5281
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 26.71"
                     /tool="antismash"
     gene            complement(5452..5898)
                     /locus_tag="I6L50_RS19265"
                     /old_locus_tag="I6L50_19255"
     CDS             complement(5452..5898)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="I6L50_RS19265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_19255"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_219302433.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAIMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVTCSIKQFLGVIENQWQAKETLHHEINHIFEIDSNELKSDLTPISKESH
                     LAFHWIDFNKENINSYTIMPTPSVKELLERKLSDELLTCWISNF"
     gene            complement(6271..6840)
                     /locus_tag="I6L50_RS19270"
                     /old_locus_tag="I6L50_19260"
     CDS             complement(6271..6840)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002065694.1"
                     /locus_tag="I6L50_RS19270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_19260"
                     /product="DUF4256 domain-containing protein"
                     /protein_id="WP_002085300.1"
                     /transl_table=11
                     /translation="MKESNKKELSLEQREELLQTLKDRFENNMNRHKGLEWANVQERLD
                     ANTEKLWSLNEMERTGGEPDVVDFDKEKGEYIFYDCSAESPKGRRSVCYDLEALESRKK
                     HKPENNAIDMATTMGIELLTEEEYRTLHNLENFDMKTSSWVQTPSDIRELGGALFCDYR
                     FGHVFVYHNGAESYYAARGFRGSLRV"
     gene            complement(7102..7566)
                     /locus_tag="I6L50_RS19275"
                     /old_locus_tag="I6L50_19265"
     CDS             complement(7102..7566)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136577.1"
                     /locus_tag="I6L50_RS19275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_19265"
                     /product="N-acetyltransferase"
                     /protein_id="WP_219302435.1"
                     /transl_table=11
                     /translation="MNVQLKIVTRENWEDALKLQVKENQIHFVPAVAVSLAKVYIKPDG
                     ENVEYIPLAIYDGDLIVGFIMHAFVRESTDMYWINGFIIDEKEQGKGYGKAALQEIINL
                     IKNNFKECEEIRLTVHKDNISAKKLYECYDFKPLGNVYDGEEVYRLLLLK"
     gene            complement(7644..8567)
                     /locus_tag="I6L50_RS19280"
                     /old_locus_tag="I6L50_19270"
     CDS             complement(7644..8567)
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="I6L50_RS19280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_19270"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_219302437.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATKPLTNEITTYYPEIHGPEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGEDLTIVDVGRSTSLAIAFNLWNDLMLNVKGIYFMGGVFLEAGNVTPLA
                     EANVYGDPIASQIVFQQAKNITLFPLNVTNKTVLTPNVFTYILANTKNPFKPIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVLAISGLVNPTFLNYTSRKVTVDICGETKGQTFADFRLH
                     SKSEGARIALQLDEEKFIQDFMKIML"
     misc_feature    8817..8833
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.88"
                     /tool="antismash"
     gene            complement(8851..9762)
                     /locus_tag="I6L50_RS19285"
                     /old_locus_tag="I6L50_19275"
     CDS             complement(8851..9762)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136580.1"
                     /locus_tag="I6L50_RS19285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_19275"
                     /product="DMT family transporter"
                     /protein_id="WP_219302439.1"
                     /transl_table=11
                     /translation="MHNKKWDFRIVCAHAFTILIWGTAFPGIRMGLEAYSPEHLTLLRL
                     LIASLILLLFSLIYKLRLPDLKDIPVIFIFGALGFTIYHIALNYGEQSVNAGPASLIVS
                     VTPILTAILASVFMNEKMKLNGWIGGVISLVGIAFLSLSQGDSIQLNSGVLLILLAAFS
                     ESLFFVFQKSYLKKYGFLPFTIYAIWSGTVCMLIFLPGIYQEIVAAPMEISLSVIYLGV
                     FPTVLPYIALAYITSHAGASEATSSLYLTPVTAGFIAWIWLGEVPTFVSIIGGIITILG
                     VVIAHLTFKKDNYKQIESEKVW"
ORIGIN
        1 aatttaggta tcatctctcc tacgccgtaa ttatagtcac ttagaatcca agatacctct
       61 ttccaatcta gaattccttc tgagactttt tttggagtgt gatatgtaaa ttcattaagt
      121 agctttacta aatagacata catgccacca gaatatgagg aatcctccca tttaataatg
      181 cctttaaatt gaatttgatt ttgttcaaca tctatatttg tttcttcttt tatttcacga
      241 attgcatttt ctaacggtgt ttctcttttt tatatcttcc ctcctacacc gttccataat
      301 ccttttacag gatcatattc tctatttagc ataagtattt cttcatttct ttttataaaa
      361 cataaagtgt gcttgtatat aatttaattc cttcctttgt tctcaataag ttctaacagg
      421 cgatattaaa tatactgctg cagattgttc acctgcttca attaatatcg gtctcatgga
      481 accaccaaaa cttaaagtaa ctctatcttc ttttattgct tttaaagtat cgaccatata
      541 tcggccatcg aaagatatat ttaattgctt ttcaccgtga atcgcatcta tttgttgtgt
      601 ctcgcatatt ttaccaatct gagaagcgtt agaagagatt tgtattgtgc attcgtcgat
      661 gatttctaag ttaacgttgt tgtttgccca ttcactagcc aataaacttg accgatctac
      721 accttgtaaa atcttttttc tatctatatt aataaccgtt tgggattcat ttggaattag
      781 gccggatata ttaggatatt ttccttcaat aagccttgaa tacaacgtaa ttgtaccgaa
      841 tttaaaaata atgtgactct ctgaaagata tatgtatact aaatttgaat tattgttcat
      901 taattttagt agttcactaa tagtggaact tggtacaatg caatttgctt tcgcatgaga
      961 tgaaattaat atttcacgta aagccagtct atgtgaatca gtcgcagcac aaattaattt
     1021 gttatgatca ataacgaaat gtacgccagt aagaactggt ctagattcat ttttagcagt
     1081 tgcaaagaca gtttgtttga atacctcaat cagctgttcc gtttctactt gtatttctgt
     1141 atgtgcgtct aaaaatggta cattaggaaa ttcatttgcc gagagtccat ttaagtttaa
     1201 agtaatttca tctgattgaa ttgtaatcaa ctgctcattc atacttttta ttaaaatctc
     1261 acttggcatt ttcttaataa tttcaatgaa atattttgct ggtacaacaa tactccctgc
     1321 ttttaaaata gttgtaattt gttcgtcttc aattgaaata ggtataaatt tttcaatgaa
     1381 aatattcgaa ttacttgcga ttaaagtaat tccagattga tctgctgtta tctttattcc
     1441 ggataatata ggaattaaag tttttgttga gatagcttta cttacttctg aaagagcttg
     1501 tgtaaattgt ttgtgattaa cgataaactc catttttccc ccgccttaaa tgaaatacta
     1561 tctttatgaa gattgatgaa taatagttcc attaaaaata taaggaactg tttctttctt
     1621 ttgtaaaagg ctttttaagt gttctggtat acgtaaataa ggttctgctg aatctaattc
     1681 catccaggta atctcctcaa tctcttttgg ccgtgatata cagagttccc ctccgattat
     1741 ttctcctaaa aaattaaaga agattgcatg atgtcctctc tcttcaaaaa aggcttcact
     1801 aatggaacaa acccctttta cggatatatc tagccctgtt tcttctttca cttcacgaat
     1861 tgctgcctct tctaacgttt ctccgaattt aactgctcca cctggtaaag tgtaataaga
     1921 accgttttcc cctttgtttt taaccattaa tagcttttca tgagtttcat catatagaag
     1981 tgcgtatgta acatttactt ttttcatatg taaccccctt gaatggtaaa aattttctgt
     2041 ctttatttta ttctatcatt aaattgtttg gattcataaa aagtaatttg agtaaataac
     2101 taatacgaaa cgaattagat tttttgaatg gcatccattg tttagacatt atgctgtaat
     2161 gaaaaagagc cctagtataa ggactctttc ttagcattaa gctatttttc gatttaacga
     2221 tttgatttct ttcttctttt ctgcatttgg agggcggatt actatagaga taacgaaaga
     2281 tataacacat agaacaccga ttgtaatgaa ggttggctga aatccgccaa gtagtgctcc
     2341 aatgaatgat cctgcaagtg caccgaatcc gaaaccttgg taaacaatcc cgtagtttgt
     2401 actatggttt tttaatccga agaaatcccc gacaatggct gggaatatgg tgatattacc
     2461 accaaagcaa aaggcaacgc ttgctacaca agcaaaataa atcccgtaat ttagtggaat
     2521 aaaacttaaa gtgaagacgg ataatccaat aataataaat gtagccgaga cgatttttaa
     2581 tcgacctatt ttatctgata acgttccaag aataattcta cctattgtat taaagattgc
     2641 aatcattgcg acggcattag cggccgtcgc tgtactaaga ccaacgagct gcaccccaat
     2701 gtcttttacc ataccgatta aatataaacc acccatacag gaagtgaata gcataaaaaa
     2761 caagaaatat acttgtttag tgcgcatcat ttcacgcgga gtatagtcat tgtgtaatgt
     2821 ttcagccgta gtgttactta caattgcttc acgtaagaag aacgatccga ttaatactaa
     2881 aaataagacg ataatacccc aatataaaaa tgcttgtgat acaccaagac tatcgataag
     2941 atttccgttt acatatctaa aaattaagct tcccattcca tatgctgaaa cagatatacc
     3001 agaaataagc cctttacggt ttggaaacca tttaattaaa ttagatagtg atgtaatata
     3061 tgctgttcca tcagcgtatc caacgacaac accggctaat aaataaagta atggtaatga
     3121 ggaaacttgt gaactaagta ttaaaccgag tcctaaaaca attcctgcag tagcgataag
     3181 tttacgaagt ccaaattttt gttgtagctt tcctgcaaat agagttgaga atgataaaga
     3241 aaaactcgta attgagaacg tgatcgcgac tgaattgaga ttccatccaa acttacttac
     3301 aaggggttga ttaaataaac tccatgtgta aattgttcct aggccaattt gaacaatgat
     3361 tgtacctaga acaattagca atggatttat agatgtttgt ttcatgctaa ctgtcccctt
     3421 cttttatcat gacatgaatt ttcacagtta gtatatccat gataaattgc aagatactgt
     3481 acggaaaatg gaaattgagt tagcttacac aaaaagacac ttcatcaaga agtgcctttt
     3541 tgtaggttat atacagcatt taaagaaagc tttgttaaga taagacattt tcgaatgctt
     3601 atgtatatat gttatcaaat aattcgctga aagtgatgag acttaagacg tatttttctt
     3661 tcataaataa gtcggagaga aaattaatat tcaatttcct caaagtcttt cggctgcggt
     3721 acagtttgca tattagctgc atctttcgga tcgctataaa tcgcgttttg ttctgtgttt
     3781 acatccttta tattttgctt tttattttta ttcacttcat ttttcattaa agttcactcc
     3841 tttttattat aggagtagtt ataccctaac catattattt ttactgctac taaatgtatc
     3901 agcgactaaa ttgctaaatt cacgtaaata atataaataa ttatccccac catagttgta
     3961 tcttttattg tacattttag caacgacgac attatattct ggaatgacta atatcgttgg
     4021 tcctgtaatt cctaatattt gataggaacc tttaggaact cgctcaccaa gttcgctaaa
     4081 taaagcaggt tcattttgaa cgaaccaaaa caacccgttt tgaggtagtt ctttatttgt
     4141 atactctgga ctttgtaagc tcgtagcaag tgctataact tcttttggca caatttgttt
     4201 accatttaaa aaacctttat ttaaatgcag gttcccccag taggcaaatt ctctcgcgga
     4261 gacaaaaaga tttttctcgg agccgtcatc tactgcacca acactactta ttgcatcttc
     4321 atttggattt acaataacct taactaaatt ttcatcatgt tctattctcc atccagtttc
     4381 attgaagttg gcaggtagga atacacgctc ctttagtaat tcaggaaaac tcttattata
     4441 cagctgataa ataagctgcg tcaccattct tacattaata tctctgtacg cccaagcttg
     4501 tcccggctca aattcacgaa aagctgtccc atcatcggtt tcatttaaac cgtgtgaatg
     4561 agttactaaa tgtcttatcg ttgtctttcc aagtaatgaa ggatcatatt ctttaaaata
     4621 atttacagcc tcgtcatcaa gagattttat tttcccctca taaactgcat aagctaccat
     4681 taatcctaaa tagctttttc ttgcggaagc gacattgaat tgtgaagata tagttacttt
     4741 tttactagta ggagtatttg aatgcgttcc actataatgc tctagcacaa tcttattatc
     4801 tttcataata caaagtgctg ccgtagaact ttgatttgtc tctttaatgt tttctaccca
     4861 tgaaattaat ttttcatatt tgtacaaata aatatcctcc taaaacatat aaaaataaca
     4921 gcatgaaaga aaagtttcaa tacattataa ctttgcatct gcacttttct ttcatgtgaa
     4981 atattataga tctataatac ttaaataaac caccagttcc agcaattaga acagctgaaa
     5041 caaccaacac aacctacgca accgccacaa cgtccgccgc cacaaccacc acagcgacca
     5101 ccacaaccgc cacaacgtcc gccgcaacga cgtgcatcgt cttgaatgta gtaatatgga
     5161 tattgatttt gttgctgatc ccaatacaca acattaccag gttgataagc attaaggttt
     5221 aacgcttgta gttcttgttg aaactcattc attatgaaac cctccattta caaatgtaaa
     5281 ttccgtccaa attttcgagt ttatttactt ttaaaaataa agtagaaata aggctataga
     5341 ctaaatgcgt acatcaacaa aatatgactt tacactaggt gatgtacctt gtttatgagc
     5401 ctattttctt tatattcttt tagtggacgg aattaatata aatagagaca attaaaagtt
     5461 actaatccag caagttaata gttcatcact taattttctt tctagtaatt cttttactga
     5521 aggtgtcggc attattgtat aggaatttat attttcttta ttaaaatcaa tccagtgaaa
     5581 tgctaaatgg gattctttag atattggagt taaatcggat tttaactcat ttgaatctat
     5641 ttcaaaaatg tggttaattt catggtggag ggtttctttg gcttgccatt gattttctat
     5701 gacgcctaaa aattgtttta tactgcaagt tactccaagt tcttctcgta actctcttat
     5761 tagtgcattc tctgctgatt ccccaatttc aacatggcca ccgggtaaaa aatagtgatg
     5821 tcctatgtat tcagcaacta gtaatttttc atcttttatc atgatagctc gtacaatatg
     5881 atggaattta cttttcattt tgtattcctc ctaatcattc actttatact tgctcgaaaa
     5941 ttttcattta aaagcgggat aatataaatc ataggtgttt catatgggtg aatttcattg
     6001 ataattgcaa gagtaccttt tacaagttct cgtttatacg ttaattaaaa ctaataattt
     6061 tgataaacga ttctaaaaaa gagtatgttt atgaagaaaa ttatcaaaac atactcttaa
     6121 tatttttagt tgattcattc ttttacacta aggtttgatt agccctattc tttgcggcct
     6181 caaaatcgaa gccgtagcta aagtatgcaa tcactactat tccctaagca aatctattct
     6241 ctacttcaaa tttacctgcc tgtacaaatt ttagactctt aacgaaccac gaaagcctct
     6301 tgcggcatag taagattctg ccccattgtg atacacaaag acgtgtccaa agcgataatc
     6361 gcaaaaaagg gcaccgccaa gttctctaat atcagaaggt gtttgcaccc aactcgacgt
     6421 cttcatatcg aaattttcaa gattatgtaa tgtacgatat tcttcttccg ttaatagttc
     6481 aatccccatg gtagttgcca tatcaatagc gttattttct ggtttatgtt tctttcttga
     6541 ttctagcgct tcaagatcgt aacaaacact tctacgacct ttaggacttt ctgctgaaca
     6601 atcataaaaa atatattcgc ctttttcttt atcaaaatca acaacatcag gttcgccacc
     6661 agttcgttcc atttcgttga gcgaccacaa cttttcagta ttagcatcca gcctttcttg
     6721 aacattagcc cattctaaac ctttatgacg gttcatatta ttctcaaaac gatctttcaa
     6781 tgtttgtagt aattcttcac gttgctctag tgacaactct tttttattgc tttctttcat
     6841 attagttcct ccattgatgt ttttattaat gatagtttaa gtatatataa atactgtttt
     6901 ataataaaat gtttaaataa ttgcgtgaat tgagtttgcg ataatttata tgaattagta
     6961 caaccaattc tccgcaatat gaccctttaa cggaacaact tattgtttca aaacagtttc
     7021 ttaatcgatc tttcaattct agatgttctt ttccttttca ctataaatac acgatataac
     7081 tttattcaaa acagtacatc tttatttcaa caacaataag cgatatactt cctcaccgtc
     7141 atatacattt cctaatggtt taaaatcata acattcatat agtttctttg cagaaatatt
     7201 atctttatgg acagttaatc taatttcctc acactcttta aagttatttt ttattaagtt
     7261 aatgatttcc tgtaatgctg cttttccata tccttttcct tgctcttttt catcaatgat
     7321 aaatccgtta atccaataca tatctgttga ctctcgtaca aaagcatgca taataaaacc
     7381 aactataagg tcaccatcat atatagccaa tggaatatat tcgacatttt caccatctgg
     7441 ttttatgtat actttagcaa gtgatactgc cactgccgga acaaagtgta tttggttttc
     7501 tttaacttgt aactttaatg cgtcttccca attttctctc gtaacaattt ttaattggac
     7561 gttcactatt ttaagctcct ttaaaaagag aatttatctt ttatattcaa cggagtgaaa
     7621 tgattccctt tttcgacatc aacttataac atgattttca taaaatcctg aataaatttt
     7681 tcctcatcta attgtaacgc tatacgcgcc ccttcagatt tagagtgtag gcgaaaatca
     7741 gcaaaagttt gtcccttcgt ttcgccgcaa atatccacag tcacttttcg ggatgtataa
     7801 tttaaaaatg taggattcac taaaccgctt atagcaagta catcgtgtaa taatggccct
     7861 tcaatagatg gattgagttt ttggtatgct gataaataat aatcgtacat gggtttcata
     7921 atcggcttaa atggattttt tgtattagct aaaatataag taaatacatt tggggttaaa
     7981 actgttttat ttgttacatt taaagggaat aacgttatat ttttcgcttg ctgaaataca
     8041 atttgacttg ctataggatc tccgtacacg tttgcctctg ctaatggagt aacattccct
     8101 gcttctaaaa agacaccacc cataaaataa attcctttta catttaacat caagtcgttc
     8161 cataaattaa aagcaatcgc taacgaggta gatcttccta catcgacaat tgttaaatct
     8221 tctccatact ttacaagaat cgcagcaata ctaccaaaat tgtatatcgg aatagataac
     8281 acactttccg gtacatgtat gggtcctaat ccttctggtc catgaatttc aggataatac
     8341 gttgtaattt catttgtaag tggctttgtt gccccgctaa ttacaggaat atcttgtctg
     8401 tttgctaact gtaaaatata tgcggcatta tgagcggcat gtatatgttc aacatttcca
     8461 tatccagtta cgataccaac aatttcaatt tcaggatgca gtaagccata cataattgca
     8521 aacgaatcat caatacctgg gtctccaaaa aataaaacct tctccaagaa atgcactcct
     8581 ttcataggta agataattat gtatatgtgg aaataattta aatctataca gttctaagca
     8641 aatattaatg gtgagtaatg tattgataaa aaagaactag cattatgcta gttcttttgc
     8701 agataaataa tctaccggtt taattaaatc tcgtacaaat attggactaa ctgattttaa
     8761 tatagattat ccccactata tttatagaat tgaaagcctg ttattaatcc ggaattttgc
     8821 attcaaattg caaatactcg cagggatgtc ttaccatact ttttcgctct cgatttgctt
     8881 ataattatct tttttaaatg ttaaatgagc aatcacaact ccaagtatag taattatccc
     8941 tccaattatc gaaacaaagg ttggcacctc gcctaaccat atccacgcaa taaaacctgc
     9001 agtaactggt gttaaatata gagaactcgt tgcttcagaa gccccagcat gagatgtaat
     9061 atacgctaat gcaatatatg gaaggactgt cggaaacaca cctaaatata taacgctcaa
     9121 actaatttcc ataggagctg ctacaatttc ttgataaatc cctggtaaaa aaataagcat
     9181 acatacagta ccagaccaaa ttgcatatat tgtaaatggt aagaaaccgt acttttttaa
     9241 ataagacttt tgaaaaacga aaaatagact ttctgaaaat gccgctaata atattagtaa
     9301 taccccacta ttcaattgaa tggaatctcc ttgacttaat gataaaaaag caattcctac
     9361 taaactaatt acaccaccaa tccaaccatt taatttcatt ttttcattca taaaaacaga
     9421 agcaagaatt gccgttaata taggtgttac tgaaacaatt aaactagcag gtccagcatt
     9481 tacactctgt tcaccgtagt ttagtgcaat atgataaata gtaaatccta gagcgccaaa
     9541 tataaaaatt actggaatat cttttaaatc aggtaaccgt aatttgtata taagtgaaaa
     9601 cagaagaaga atcaaagaag caattagtaa tcgtagcaaa gtaaggtgtt cagggctgta
     9661 agcttcaagc cccatccgaa ttcctggaaa agctgttccc catataagaa tcgtaaaagc
     9721 gtgagcacat acaatacgaa aatcccattt tttattatgc atattttctc gccccctcca
     9781 ataaaccagc cattttctaa ttaccaatta tttcttttca tcatatttaa tagttgatac
     9841 aatgtcttca accagttaat cctcgaaaaa accaaccact ttgcattttt aaaatgaatg
     9901 agggagtttt taatatgtct actgataaaa aactgcctaa atatcggcag ataatagact
     9961 atatgaaaga aaaaattgaa tacggagaat ggccgattgg aagtaaggtt cctagccagc
    10021 ggcagttagc gaaaacattt catgtaaatc gtagtactgt aataaccgca ctagaagaac
    10081 ttatggcaga tggcttaatt caagggcaaa tcgggtcagg aacaattgta acgaataata
    10141 catggtcatt attagccacc aatcccccgc ccgattggag tgaatatgta aaggctggta
    10201 ttcataaacc aagcaagtta atggtacgag aaataaatga agcagaagca aa
//
