LOCUS       NZ_JAHWJB010000002     10297 bp    DNA     linear   CON 12-SEP-2024
DEFINITION  Bacillus sp. FDAARGOS_1420 tig00000007_arrow_pilon, whole genome
            shotgun sequence.
ACCESSION   NZ_JAHWJB010000002
VERSION     NZ_JAHWJB010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. FDAARGOS_1420
  ORGANISM  Bacillus sp. FDAARGOS_1420
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 3674589)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3674589)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3674589)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
REFERENCE   4  (bases 1 to 3674589)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T. and
            Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1
            Genome Coverage                   :: 1241.19x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019344915.1-RS_2024_09_12
            Annotation Date                   :: 09/12/2024 05:20:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,050
            CDSs (total)                      :: 6,900
            Genes (coding)                    :: 6,426
            CDSs (with protein)               :: 6,426
            Genes (RNA)                       :: 150
            rRNAs                             :: 12, 12, 12 (5S, 16S, 23S)
            complete rRNAs                    :: 12, 12, 12 (5S, 16S, 23S)
            tRNAs                             :: 109
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 474
            CDSs (without protein)            :: 474
            Pseudo Genes (ambiguous residues) :: 0 of 474
            Pseudo Genes (frameshifted)       :: 199 of 474
            Pseudo Genes (incomplete)         :: 296 of 474
            Pseudo Genes (internal stop)      :: 116 of 474
            Pseudo Genes (multiple problems)  :: 119 of 474
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:39:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1540042
            Orig. end                         :: 1550339
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHWJB010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5297](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5297
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    641..654
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.26"
                     /tool="antismash"
     gene            788..1393
                     /locus_tag="I6L50_RS18180"
                     /old_locus_tag="I6L50_18175"
     CDS             788..1393
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853587.1"
                     /locus_tag="I6L50_RS18180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_18175"
                     /product="PRK06770 family protein"
                     /protein_id="WP_219302247.1"
                     /transl_table=11
                     /translation="MKTLFKIIGIIAGMAVIGVGVTYGMLYYLNNSKPAAKKESPAAPA
                     VEVLADSNVKAEDAKMLENGNYSLPNSGFNKNFKWTDENIQTALHEMAHQKAKADQKWG
                     YIFITQERIESLLEIINSNDVAQKSTYVDILERWKQGKYEKVDVDHNKIWKLQSGNLGE
                     GKGVMSEKEQKELINQVFIQKGTYSGSKLIAGGGQTNK"
     gene            complement(1507..2376)
                     /locus_tag="I6L50_RS18185"
                     /old_locus_tag="I6L50_18180"
     CDS             complement(1507..2376)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200508.1"
                     /locus_tag="I6L50_RS18185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_18180"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_219302248.1"
                     /transl_table=11
                     /translation="MEMRHVKTFCAIVKYGNFSKAAHALGYAQSTVTAHIKSLESDLNI
                     PLFDRLGKKILLTKAGHQFHPYALELLTIYDKAQEIPQNSDHLEGTLTITSNESLAVYR
                     LPQLLRTYKQENPKVNIVLETNTNEQALKKLHEGETDVIFIIGESIEHNDFITCNLCNE
                     TFGWIFPAHFPSNTDPLLLLQNTQFIFTEQSCGYRPMVDRFLRQSGNIPAKTFETSNVE
                     VIKQSVMCELGISILPYIVVQESCQKEQLCFQPIETPAVIQSHVIYHKSRWISPVLQSF
                     LTLLERIE"
     gene            2506..3549
                     /locus_tag="I6L50_RS18190"
                     /old_locus_tag="I6L50_18185"
     CDS             2506..3549
                     /EC_number="1.2.1.11"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1219: N-acetyl-gamma-glutamyl-phosphate reductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200507.1"
                     /locus_tag="I6L50_RS18190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_18185"
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="WP_219302249.1"
                     /transl_table=11
                     /translation="MSEKSYHVAVVGATGAVGQKIIALLDRETKFNIEEITLLSSKRSA
                     GKAVKFKGREIKIQEAKSTSFEGVDIAFFSAGGEVSRQYVNHAVSSGAIVIDNTSEYRM
                     AHDVPLVVPEVNAHTLRKHKGIIAVPNCSALQMVTALQPIRKSFGLERIIVSTYQAVSG
                     SGIHAIHELKEQAKSMLTGQEVKSTILPAKKDKKHYPIAFNVLPQVDIFTENDFTFEEV
                     KMIQETKKILEDPKLKMAATCVRVPVVSGHSESVYIELEKEATVAEIKEVLLNAPGVIL
                     QDNPSEQIYPTPLYAEGKIDTFVGRIRKDPDTPKGFHLWIVSDNLLKGAAWNSVQIAET
                     LVEKGII"
     gene            complement(3657..4088)
                     /locus_tag="I6L50_RS18195"
                     /old_locus_tag="I6L50_18190"
     CDS             complement(3657..4088)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674076.1"
                     /locus_tag="I6L50_RS18195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_18190"
                     /product="hypothetical protein"
                     /protein_id="WP_219302250.1"
                     /transl_table=11
                     /translation="MNKSKPVDELTIEDLKQNPIWEWAIDEEENEECDETWVKPVETIN
                     FTEELNGSIVLGELIIHNDEKFPMMCSIDIENNEVLISSIVFYNEKENEYIAIEDVVKK
                     VEMPISINIYLTINGEPRFLKFSANKIDIYKNTIKTNLN"
     misc_feature    complement(4295..4308)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 22.5"
                     /tool="antismash"
     misc_feature    4299..4312
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: medium, score: 18.47"
                     /tool="antismash"
     gene            5001..5297
                     /locus_tag="I6L50_RS18200"
                     /old_locus_tag="I6L50_18195"
     CDS             5001..5297
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016120187.1"
                     /locus_tag="I6L50_RS18200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_18195"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_219302251.1"
                     /sec_met_domain="TIGR03601 (E-value: 3.9e-40, bitscore:
                     134.0, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKFQQELQALSLNDYQSGNIVYWDQQNQYPYYYITDDARRCGGC
                     GRCGGCGGGRCGGFRCFGCFGCFSCFGCGGCSNCSNCSNCFDGFNGTTVTFEY"
     gene            complement(5606..6508)
                     /locus_tag="I6L50_RS18205"
                     /old_locus_tag="I6L50_18200"
     CDS             complement(5606..6508)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614455.1"
                     /locus_tag="I6L50_RS18205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_18200"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_219303718.1"
                     /transl_table=11
                     /translation="MISIELRQLEYFLAVSKELHFTRAAEKLNISQPSLSQQIRALEHE
                     VGMPLFDRIGKKISLTEAGKVLLSHSKTIFHEVEQARSAIQDLNGLQHGSLTIGSLLTV
                     VNYLLPPAILNFNNLYPNIELSVLGLRTGEIREKLLQNELDIGITFLPVQDKEIVSIPL
                     YKSELTLVVPTGHRLTEHDHVSIAELQNYPLILLPKNFFLTELITSHCQKFNFTPKPIL
                     EISTMESLIQMVSKDMGITVLPKPYIDFLQSEHIQSIKIENPTPTIDIGLIYRKDKYMC
                     AATREFIEQLKLTVQSLQN"
     gene            6638..7963
                     /gene="gabT"
                     /locus_tag="I6L50_RS18210"
                     /old_locus_tag="I6L50_18205"
     CDS             6638..7963
                     /EC_number="2.6.1.19"
                     /GO_function="GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (34-374). E-value: 1.5e-121.
                     Score: 397.5. Matches aSDomain:
                     nrpspksdomains_I6L50_RS18210_Aminotran_3.1"
                     /codon_start=1
                     /gene="gabT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200502.1"
                     /locus_tag="I6L50_RS18210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_18205"
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_219302252.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.8e-131, bitscore:
                     436.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKSSVLPGSYSQVLQKRYEEAVPTGIYHLTPLYVKEARGAIITD
                     VDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAESFIHPCFHVTPYENYITLAEKINEK
                     VPGTSKKKTMFANSGAEAIENAVKIARKVTGRSKVISFERGFHGRTLMTMSLSTKETPY
                     KAGFGPFVSDMHILPYPYYYRAEEGVSTEEVDSQVIYQFEKFLEEVSPEKVAAIVMEPV
                     QGEGGFIVPSITFVKRLREICNQYGILMIMDEVQTGFGRTGKWFATEHFHIEPDIMTLS
                     KSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGSPLSCAVGLAVFEIMEEENLVERAQQ
                     IGNRIMLHLHQMQLKYEVIGDVRGIGAMVAMELVTNRITKETAKELTVKLIEDFWKNGL
                     ISIGAGIFDNVLRFLPPLVISNEEIDRGFEIINESFEKLCRK"
     aSDomain        6740..7759
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_I6L50_RS18210_Aminotran_3.1"
                     /evalue="1.50E-121"
                     /label="I6L50_RS18210_Aminotran_3.1"
                     /locus_tag="I6L50_RS18210"
                     /protein_end="374"
                     /protein_start="34"
                     /score="397.5"
                     /tool="antismash"
                     /translation="VKEARGAIITDVDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAE
                     SFIHPCFHVTPYENYITLAEKINEKVPGTSKKKTMFANSGAEAIENAVKIARKVTGRSK
                     VISFERGFHGRTLMTMSLSTKETPYKAGFGPFVSDMHILPYPYYYRAEEGVSTEEVDSQ
                     VIYQFEKFLEEVSPEKVAAIVMEPVQGEGGFIVPSITFVKRLREICNQYGILMIMDEVQ
                     TGFGRTGKWFATEHFHIEPDIMTLSKSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGS
                     PLSCAVGLAVFEIMEEENLVERAQQIGNRIMLHLHQMQLKYEVIGDVRGIGAMVAMELV
                     "
     gene            7978..8667
                     /locus_tag="I6L50_RS18215"
                     /old_locus_tag="I6L50_18210"
     CDS             7978..8667
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002031964.1"
                     /locus_tag="I6L50_RS18215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_18210"
                     /product="MOSC domain-containing protein"
                     /protein_id="WP_002085626.1"
                     /transl_table=11
                     /translation="MTKVYEILSLNLGLPKEVMYGGKLIQTGINKKQVKEPVFLSFLKF
                     NGDGQADLVHHGGVDKAVCVYTGDHYPYWEKELNQELVYGAFGENITVSGMREEDVCIG
                     DTFQLGEAIVQVTQPRQPCFKLAKKYNIPKLPLYFQDTGYTGFYFRVLKEGWILPVDTL
                     KLLKPDPKGVSILFANRIMHKEKQNSEGLKRILEVNALSTSWRKTFEKRVKGEEISTKE
                     RLEGTKE"
     gene            8930..9813
                     /locus_tag="I6L50_RS18220"
                     /old_locus_tag="I6L50_18215"
                     /pseudo=""
     CDS             8930..9813
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526760.1"
                     /locus_tag="I6L50_RS18220"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="I6L50_18215"
                     /product="GNAT family N-acetyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGNVKKWQTFY"
     misc_feature    complement(9897..9913)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.8"
                     /tool="antismash"
ORIGIN
        1 gaaagaccaa aaaatgaaca tagaacatgg tattttgaaa aacaagtaga gcgctggaat
       61 atagcaatgc agaagtcgca gcaatatgag ttagtaatac tcgatggtga tatttatcag
      121 ccacttagtt ataattggtg tttccatttt gatatattta atcagccgtt atctttaata
      181 gaaaactttt ataaagaaaa gataattaac gaagaaattg gttttccaga tcaatatttt
      241 tatttatata caagtgatga agagcttaga aaacggaaag agtctgatgg gacgaaaaaa
      301 agaagaaact ttgaaaaaca tttacatata tctaaagcat ttcagcgtta ttatgaaaat
      361 ttaaataacg tcacagacgg gtactgtaaa ttgattgaag caaaaagtat aaagtcgaat
      421 gaattagaaa ttgtgaaaag tttaaacagc ttaaacgtgt gtgaagaaag acgttttgat
      481 gtttcgaggt tagatgctat tacggagtgg ttaaaagaga atcgagcata aagtaaaaac
      541 aacgagtgtt ttagaaggcg ctcgttgttt ttttgtttcc atataaatat aaagggaaat
      601 tttgtagaat atctttattt tttaacagaa taatctttca attttacaaa aaaagaaata
      661 caatataagg aaatatatat aaataatatg atagggggtg ggatgaacta ttttgaatga
      721 aggaactgtt ttatataaga aataatgagg tagtaagaac agagatattt gggaggaaac
      781 aagcaagatg aaaacattat ttaaaatcat aggaattata gctggtatgg cggtaatcgg
      841 ggtaggtgta acgtatggta tgctgtatta tctgaataat agtaaaccag ctgcgaaaaa
      901 ggaatcacca gccgctccgg cagtagaagt gctagctgat agtaatgtaa aagccgaaga
      961 tgcaaagatg ttagaaaatg ggaattattc attgccgaat agtggtttta ataaaaattt
     1021 caaatggaca gatgagaaca tacagacagc attacatgaa atggcacatc aaaaagcgaa
     1081 agcagatcaa aaatggggat atatctttat tacacaagaa cgtattgaaa gtttacttga
     1141 aattataaat agtaatgatg ttgcacaaaa aagtacatat gtggatattt tagaacgatg
     1201 gaaacaagga aaatatgaaa aggttgatgt ggaccacaac aagatttgga aattacaaag
     1261 tgggaattta ggagaaggga aaggagtaat gtcagaaaag gagcaaaaag aattaattaa
     1321 tcaagttttt atacaaaaag gtacatattc aggtagtaaa ttaattgcag gtgggggaca
     1381 aactaataaa taaggtacat attgattttg aaacacctgt attgttatga acacaaatgt
     1441 tttaataaaa atgagtaatt gctttttaga gcagttactc atttttgtaa ttactcgctt
     1501 acttttctat tctattctct ctaacaatgt aagaaacgat tgtaaaacgg gtgatatcca
     1561 tctagattta tgatagatta catggctttg aatgactgct ggagtttcga tgggctgaaa
     1621 acagagttgc tctttttgac aactttcttg tacaacgatg taagggagaa ttgaaattcc
     1681 taactcacac atcacagatt gtttaatgac ttcaacattg gaagtttcga atgttttagc
     1741 gggaatattc ccactttgac gtaaaaaacg atctaccatt ggtctatagc cgcagctttg
     1801 ttcagtaaat ataaattgag tattttgtaa taaaaggagt ggatcagtgt ttgaagggaa
     1861 atgagcaggg aaaatccatc caaatgtttc attacataaa ttacacgtaa taaaatcatt
     1921 atgctctata ctttctccaa ttatgaagat aacatcggtt tctccttcat gtagtttttt
     1981 caatgcttgc tcattggtat ttgtctcaag tacgatattt actttcggat tttcctgttt
     2041 gtaagtacgt aataattgtg gtaaccgata cactgccaat gattcattag atgtaatagt
     2101 caatgttcct tctaaatggt cactgttttg agggatttct tgcgctttat cataaatagt
     2161 aagtaattct aatgcgtacg gatgaaattg atgacctgct tttgtaagaa gtattttttt
     2221 tcctaatcgg tcgaatagag gaatgtttaa atcgctctct aatgatttaa tatgagctgt
     2281 cacagtagat tgcgcataac ctaatgcatg agcagcttta gaaaagttgc catatttaac
     2341 aatggcacaa aatgttttaa catgtctcat ttccataagt gatacctctt ttattatcat
     2401 gatttgtgaa tgattatatc atgtatttca atttttcaaa tcattaaatt tcttttaaaa
     2461 ttaagttgtg gagaaattta ttcaagtgaa attggaggag aaaaaatgag tgaaaagagc
     2521 tatcatgtag ctgtagttgg ggctacaggt gcagtagggc aaaaaattat tgcattgtta
     2581 gatagagaaa cgaaatttaa tatagaagaa attacattac tctcgtcaaa acgatctgct
     2641 ggtaaggcag taaaatttaa aggaagggag attaaaatac aagaggcaaa atcaactagt
     2701 tttgaaggtg tagacattgc attttttagt gctggaggag aagtttctag acaatatgta
     2761 aatcatgcag tttctagtgg tgcaatcgta attgacaaca caagtgaata ccgaatggca
     2821 cacgatgtac cactcgtcgt tccagaagta aatgcacata ctttaaggaa acataaaggg
     2881 atcattgcag ttccgaattg ctcagcacta cagatggtaa cagctcttca gccaattcga
     2941 aaatcatttg gtttagaacg aattattgtt tcaacgtatc aagccgtatc aggttcagga
     3001 attcatgcga ttcatgaatt aaaagaacag gctaagtcaa tgcttacagg tcaagaagtg
     3061 aagagtacta tattaccagc gaaaaaagat aaaaaacatt atccaattgc atttaatgta
     3121 ttgccgcaag tggatatatt tacagaaaat gatttcacat tcgaagaagt aaagatgatt
     3181 caagagacga agaaaatttt agaagatcca aagctgaaaa tggcagctac ttgcgtcaga
     3241 gttccagttg tatcggggca ttctgaatcg gtttatattg aacttgaaaa agaagcgacc
     3301 gttgcagaaa ttaaagaagt actactgaat gcaccaggtg ttattttaca agataatcct
     3361 agtgaacaaa tttatccaac accattatat gcagagggta aaatagatac atttgtaggt
     3421 agaatacgca aagatccgga tacgccaaaa ggattccatc tttggattgt ttctgataat
     3481 ttattaaaag gtgcggcttg gaactcagtt caaatcgcag agacattggt ggaaaagggg
     3541 attatttagt ataaatttat ataaatattg aaaataggag gatatatggg aaaccgttaa
     3601 ttataatggt ttcccatttt ttacttaatg agtattctcc taacttcaat tagaatttaa
     3661 tttaagttag tttttatagt gtttttataa atatcaattt tatttgcaga gaattttaaa
     3721 aatctaggct ctccattaat tgtgagataa atattaattg agattggcat ctcaaccttt
     3781 ttcactacat cttctatagc aatatactca ttttcttttt cgttataaaa aacaatggaa
     3841 ctaattaata cctcgttatt ttcaatatca atactacaca tcatagggaa tttctcatca
     3901 ttatgtatta ttaattcgcc cagtactatt gaaccgttta attcttctgt gaaatttata
     3961 gtttccactg gttttaccca cgtttcatcg cactcttcat tttcctcttc atctattgcc
     4021 cattcccata tcgggttttg tttcaagtct tcaatagtca attcatcaac tggtttgctt
     4081 ttattcataa gtactttcct ctcttaacat aataaatgga tgattcaaaa atttattctt
     4141 taactgttca atgtggctat ttcaatctta tacatacagg tttgctcgtt tcaaaaaata
     4201 gttgatcgcc aataagaagt ttcaatatag agctttattc atttggaact atttaatcta
     4261 gttttgaaat gttatacgca cattaataat aatcagaatg tatgttcttg caacgatttt
     4321 aacaagattt tgagtagaca tcaaataata tttattttag tcagctgaaa agaaaaaagg
     4381 actgtgataa aagaggttaa atgataaaaa taagggttgg aaatacaccg gagataaaaa
     4441 taaatgaaga gaagttaaaa aagtaagaag agcttacgca gatttataaa cagaagcata
     4501 atggaaaaat accgttcaaa attagctctg atatagcagt tattgaagcg aagaaaaaga
     4561 tgggagtgca tattggaaat acgaagatga ccaaacaaaa ttatggaata tacgactata
     4621 gaactagaaa atgataacat aaatctaaat gttattgttt aaattcatat tttataattt
     4681 ccatttgtaa aaatgagccc gtagtaaaaa caacctaaaa ataggcatat gaacaaggta
     4741 catcatctag tgataggtca tattttgtag gtgtacgtat tgaacttagt ttaacccgct
     4801 atttgtgggc agtaaaactc ccacctcaaa attcgactgg agcaaagaag ttaggtggga
     4861 gtcgggctaa taatcagtgg gggatgaaca ctccccactg attaaagttt cactttaagc
     4921 gctatctcta cttagattta acgaacagaa atataggaaa gcatcgattg ggttgtagtt
     4981 tataaacgga ggtcatgaaa atgaataagt ttcaacaaga actacaagcg ttaagcctta
     5041 atgattatca gtctggaaat attgtgtatt gggaccagca aaaccaatat ccatattact
     5101 atattacaga tgatgctcgt cgctgtggcg gttgtggtcg ttgtggcggt tgtggcggtg
     5161 gtcgttgtgg tggattccgt tgttttggtt gcttcggttg ctttagttgt tttggttgtg
     5221 gtggttgctc taattgttct aattgctcta attgttttga tggttttaat ggtactactg
     5281 taacatttga atattgataa gagacaaaaa ggaatccatt ttgaaaaaag attgatcgaa
     5341 acggattccc actttgtgaa gtactatata atttttataa aaatgtgcta acaagattgc
     5401 aaacggactt gctaacacct atacgaaata gacataaaat atcgttgatg tgtagcttaa
     5461 taatacgata ataatttagc acttataatg aataataggt tattagaaaa gaaaccataa
     5521 aatgtaatgt tttatattat tataaaaaga gattattcta atagggcgtt gtgtaacggc
     5581 tacataggat aatctctttt tatgtttaat tttgtagaga ttgtactgtt aactttagtt
     5641 gttcaatgaa ttctcgggta gcagcgcaca tgtatttatc ttttctataa ataagtccta
     5701 tatctattgt tggagtgggg ttttcaattt taatagattg aatgtgttcg ctttgcaaaa
     5761 aatctatata aggttttggt aatactgtaa ttcccatatc ttttgagacc atttgaatta
     5821 aagattccat cgtactaatt tctaaaattg gttttggtgt aaagttgaat ttttgacaat
     5881 gagatgtaat tagttcggtt aaaaagaaat ttttaggtaa aagaattaaa gggtagttct
     5941 gtaattcagc aatagataca tgatcatgtt cagttaatcg atgaccggtc ggcacgacta
     6001 atgtaagttc acttttatat agtgggatag aaacaatctc tttatcttgc actggaagaa
     6061 atgtaatgcc aatatctaat tcattttgta ataatttttc acgaatttcc cctgtacgaa
     6121 gacctaatac agaaagttca atattcggat ataaattatt gaaatttaaa atggctggtg
     6181 gtagtagata atttacgacg gttaacaatg agccgattgt taaggatcca tgttgtaatc
     6241 cgtttaaatc ttgaatggca gagcgggctt gttcgacttc gtgaaagatt gttttactgt
     6301 gcgataataa aacttttcct gcttcagtta aagatatttt tttaccaatt cgatcaaaaa
     6361 gtggcattcc tacttcatgt tctaatgcac gtatttgttg gcttagtgaa ggttgtgaaa
     6421 tatttaattt ctcggctgct cttgtgaaat gtaattcttt cgaaacagct aaaaagtatt
     6481 ctaattgtcg taattcgatt gaaatcacct caagtttaat cataggatat acctatcatt
     6541 attatataaa taattgaatt gaacaatggt tttgtgggat atataatcac tttatcagaa
     6601 atggattgat taaaaaatta gatgaggtga agaagaattg ttgaaatcta gtgtattacc
     6661 gggcagttat tcacaagtac ttcaaaaaag gtatgaggag gctgtaccga caggaatata
     6721 tcatttaacg cctttatatg taaaagaagc aaggggagcg ataataacag atgtagatgg
     6781 aaatcagttt attgattttg ctggtggaat tggtatgcaa aatgttggac attgtcatcc
     6841 gaaagttgta agagcggtac aaaaacaagc agaatcattt atacatcctt gttttcatgt
     6901 aactccatat gaaaattata ttactttagc ggagaaaatt aatgagaaag tgccaggtac
     6961 aagtaagaaa aaaacgatgt ttgccaatag tggtgcggaa gcgattgaaa atgctgtaaa
     7021 gatcgcacgt aaagtaacag ggaggagtaa agtaatttcg tttgaacgag gatttcatgg
     7081 gagaacatta atgacaatgt ctctatcgac caaagaaact ccatataaag caggatttgg
     7141 tccgttcgtt tcagacatgc atatattgcc gtatccgtac tattacagag ctgaagaagg
     7201 agtttctact gaagaagtgg attctcaagt catctatcag tttgaaaagt ttttagaaga
     7261 agtatcacct gaaaaagtag cagcaatcgt aatggaacca gtacaagggg aaggtgggtt
     7321 tattgttcct tctattacat ttgtaaaacg attacgagaa atttgtaatc aatacggtat
     7381 tttaatgata atggatgaag tgcaaactgg atttggccga acagggaaat ggtttgcaac
     7441 agaacatttt catatagagc ctgacataat gactttgtca aaatcaattg ctggcggttt
     7501 accattaagt gctattgtcg gtcgtgcgga gttgatggac attcctggga gaggacaaat
     7561 tggtggaact tttgcaggta gtccgttatc ttgtgcagtt ggtcttgctg tatttgaaat
     7621 aatggaagaa gaaaacctag ttgaaagggc tcagcaaatt ggaaatagaa taatgttaca
     7681 tctacatcaa atgcaattaa agtacgaagt gattggtgat gttcgtggaa tcggtgcgat
     7741 ggttgcaatg gaacttgtaa cgaatcgcat aacgaaagaa actgcaaaag aactaacagt
     7801 aaagctaatt gaagattttt ggaagaatgg gcttataagt attggagcgg ggatatttga
     7861 taatgtacta cgctttttac cacctctagt tatctcaaac gaagaaatag atagaggatt
     7921 tgaaataata aatgagtctt ttgaaaagtt gtgccgcaag taagaggagg gatttgaatg
     7981 actaaagtat atgaaatcct atctttaaat ctaggattgc caaaagaggt tatgtatggt
     8041 gggaagttaa ttcaaacagg gataaataaa aagcaagtaa aggaacctgt ttttttatca
     8101 tttttaaagt ttaatggaga tgggcaggcg gatttagttc atcatggagg tgtagataaa
     8161 gcggtttgtg tttatactgg tgaccactat ccttactggg aaaaagagtt gaatcaggaa
     8221 cttgtatatg gggctttcgg tgaaaatata acagtaagcg gaatgcgtga agaagatgtt
     8281 tgtattggtg acacgtttca actcggagaa gcgattgtac aagtaacgca gccaagacaa
     8341 ccttgtttta aattagcgaa gaaatataat attccaaagt tgccattata ttttcaagat
     8401 acaggataca caggttttta ttttcgagta ttaaaagaag ggtggatatt accggtagat
     8461 actttaaaat tgttgaagcc tgatccaaaa ggtgtgtcaa ttttgtttgc gaatcgcata
     8521 atgcataaag aaaaacaaaa ctctgaagga ttaaaaagga ttttagaagt aaatgcactg
     8581 tctactagtt ggagaaagac gtttgaaaag cgagtgaagg gagaagaaat tagtacgaag
     8641 gagcgacttg agggaaccaa agaataggtg aattatattt gcataaaatg gtgtatttct
     8701 attatcggaa agataagtag gaagcaccat ttgctatata ataaacatga tattattcaa
     8761 aaatcttaat actagtttat tcgacttgtt ttttaataat caaaacataa aataaattaa
     8821 accattttta aaagttttat gatactatat tgatttgaaa ggcctatttt ttgagagtag
     8881 gtcatataag tatatctaaa ttaatggaat cgacgtatgg agggacaaca tggggaatgt
     8941 taaaaaatgg caaactttct attagaagaa ccgagaagag aacagttatt tcctttattt
     9001 gaagaagtgt ttgggattac gagccaaaca ttgcaggatt tttcagagaa agggtattgg
     9061 gattatacat ataaagcatt atcattttta caagatgata atgcgattgc aaatgttgcg
     9121 gcattttcac ttccgctttt aattaatggt gaaaaaataa atgcaacggg tattcaatca
     9181 gtaatgacac atccgaatta tcgtagacga ggattaatga aacaattgtt tagtaagatg
     9241 atagaggaaa ttgatgagca atgcgagtgt gcggtcttgt ttactgaaaa tccggaacta
     9301 tataaaccat ttggttttca aattgtgcaa gaatatttga tgacagtatc atgcgaaaat
     9361 aaaggttaca aagattctca acttaaaaag ttagattttt ataatgaaga ggatagacaa
     9421 ttaatacatg agattattga aaatggccaa aggctttcta ataaattttc aacgttaaat
     9481 taccgctctt cgttttattt aaatatgtat gattcaacat ggaatgaaaa cctttattat
     9541 tcagagaaat tagatgcgct gattgttttt acagtagaaa atcaaaaact aaaattattt
     9601 ggagtatttg cgccggtaat tccgatttta gatgaattat gtgaggaaat ctctggggaa
     9661 tttacggaga ttgaatttta tttctaccca gatcaattag gaattgatga tgtacaatat
     9721 acagaattac agactagtaa gtatttaatg gttcgaagta ataaagaatt agattttaaa
     9781 ggttataaat tcccggtatt aactgagttt taaatattaa tttaaatgct tttttatgga
     9841 ggaattatga aaacatcata tataccaaat catattgagc aacttactgt aatgaacgca
     9901 cctaaatttt acaaacttga agacaatgat acattcattg tccattcgaa agaagaaaca
     9961 gatttttggc tgaaaaccca ttatggtttt gaagtaatga atggccatgt attttatgat
    10021 gagataatga cagattttca ggctgaggtt caattgcgca tgaatccaaa tgcgacatac
    10081 gatcaagcgg gtttatttgt tatgatttct gagaagtgtt ggttaaaaac atctttagaa
    10141 tatattcctg agggcccatc acatttaggt gctgtcgtaa cgaataatgg ctattctgat
    10201 tggtcaacgc aagactatcc aaatgagttt gctaagcaac aattacgttt tcgaattatt
    10261 cgaaaacttg gcgattatac aatatatgtg aaaacga
//
