LOCUS       NZ_JAHPZL010000015     10288 bp    DNA     linear   CON 18-AUG-2024
DEFINITION  Austwickia sp. TVS 96-490-7B contig_15, whole genome shotgun
            sequence.
ACCESSION   NZ_JAHPZL010000015
VERSION     NZ_JAHPZL010000015.1
KEYWORDS    WGS; RefSeq.
SOURCE      Austwickia sp. TVS 96-490-7B
  ORGANISM  Austwickia sp. TVS 96-490-7B
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae; Austwickia.
REFERENCE   1  (bases 1 to 28794)
  AUTHORS   Bruer,T., Sarker,S., Lynch,S., Jack,C., Helbig,K., Isberg,S. and
            Tscharke,D.C.
  TITLE     Draft genome assembly of Dermatophilus crocodyli, causative agent of
            dermatophilosis in saltwater crocodiles and a proposed taxonomic
            reassignment to Austwickia crocodyli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 28794)
  AUTHORS   Bruer,T., Sarker,S., Lynch,S., Jack,C., Helbig,K., Isberg,S. and
            Tscharke,D.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2021) Immunology and Infectious Disease,
            Australian National University, Garran Road, 131, Canberra, ACT
            2601, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: SPAdes v. 3.13.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Reference-guided Assembly :: GCA_900187045.1; GCA_003391095.1;
            GCA_012277275.1
            Genome Coverage                   :: 115.0x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019331795.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 02:33:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,245
            Genes (coding)                    :: 3,183
            CDSs (with protein)               :: 3,183
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 14 of 62
            Pseudo Genes (incomplete)         :: 53 of 62
            Pseudo Genes (internal stop)      :: 9 of 62
            Pseudo Genes (multiple problems)  :: 13 of 62
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:42:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8121
            Orig. end                         :: 18409
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHPZL010000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10288
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10288
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10288
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5288](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5288
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(11..25)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
     gene            449..973
                     /locus_tag="KEM60_RS14385"
                     /old_locus_tag="KEM60_02968"
     CDS             449..973
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KEM60_RS14385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KEM60_02968"
                     /product="hypothetical protein"
                     /protein_id="WP_219108444.1"
                     /transl_table=11
                     /translation="MTIRRRVITTVAGVAAGIAIIGGAAAANADTTTSADSKGTTQQSD
                     TQCHGGGEKHRRTPVTGDEATKVGDAVKAKDAAASTDKVFKRADGSYDVLATKGDKHTH
                     FRVSADLKTVTEKTRGGHGKGGHGQGKGRPAPGEAGPGEGGPRPEGPHGEGPRDGAGTG
                     SASPQPKAYQG"
     gene            complement(981..2405)
                     /gene="brnQ"
                     /locus_tag="KEM60_RS14390"
                     /old_locus_tag="KEM60_02969"
     CDS             complement(981..2405)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /gene="brnQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503510.1"
                     /locus_tag="KEM60_RS14390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KEM60_02969"
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="WP_255574698.1"
                     /transl_table=11
                     /translation="MSDTRSSAPSPTSAAPSTTRMPRSGLSGGQSILVSSLLFGLFFGA
                     GNLIFPVELGRAAGRDVTIATLGFLLTAVGLPIVGVIASAVSHTSSVLEIASRVGRRFG
                     VFFTCALYLTIGPLFAIPRTATVSYEVGVRPLLPDSMATVALAVFSVIFLGLTALFAMR
                     PGKLIDWIGKYLNPAFLISLSAVLIFAVITPMTDGPLAAPTGAYATSPLVAGLLDGYGT
                     MDALGSLAFAVLVIDSIHRLGVRDIKGVAVQTGKAGVIAGIAMSVLYGLLAFVGATSLT
                     VAAGAANGGAILAAVSRHYFGPGGQLLMAAIVFFACLKTAIGLTTACAEMFAQVFPDRM
                     PYDRWVQIFAAISLGVANIGLAAIVKVSVPVLMFLYPIAIALILLALATRWLQHRPEVY
                     RWTIGFVCVAAVFDALRALPASISGSSTVTTLLDMVATVLPGLQSGFGWMLPAVIGATI
                     GVVLSQRRLGRVDTAA"
     gene            complement(2462..4723)
                     /locus_tag="KEM60_RS14395"
                     /old_locus_tag="KEM60_02970"
     CDS             complement(2462..4723)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774524.1"
                     /locus_tag="KEM60_RS14395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KEM60_02970"
                     /product="cation-translocating P-type ATPase"
                     /protein_id="WP_219108446.1"
                     /transl_table=11
                     /translation="MTEVAPQRHTTELLIGGMTCSSCSARVERKLNKIDGVQATVNLAT
                     EKAHIDHGDDIDIDRLISTVEAAGYHATLIDPRPAVLASDQPTDATTRDDHADTAADLR
                     RRLVVSATLSIPVLLVSMIPPAQFDYWQWLCLVLASPVALWGAWPFHRAAWVNLRHGGA
                     TMDTLVSVGVLASWTWSLWALFFSSAGDPTMRMAFELMPRHADGTEHLYFETAAMVTVL
                     ILLGRTLEAQARRRSGDALRALLDLGAKEVTVVRDGAQIRIPVESLAVGDEFIVRPGEK
                     IATDGVVLTGSSAVDTSLLTGEPVPVDVTTGDPVTGACLNTDGLLHVRATRVGADTRLA
                     QITRLVEEAQTGKAQVQRLADRIAAIFVPTVILLSMATLAGWLLLGQSTTAAFTAAVAV
                     LVIACPCALGLATPTALMVGTGRGAQLGILIKGPQILESTRRVDTVVLDKTGTVTSGVM
                     SVRDVIAAPGEDPQHILRLAAAVEHGSAHPIAAAITAHGRDTLGDLPTVTDFTSHTGLG
                     VTGTVDAVVVTVGRHDPAEAAPAELLAAADAARGDGATVVWVGTAGTPRGIIVVTDTVR
                     ATSAQAIAKCTAMGLTPILLTGDNERAALAIARDIGIDGDHVIADVMPHDKVAHVARLQ
                     KEGRVVAMVGDGVNDAAALAQADLGLAMGTGSDVAIEAGDLTLVRGDLLSAVDAIRLSR
                     RTLATIKGNLFWAFAYNGAAIPLAVVGLLNPIIASGAMAFSSVFVVTNSLRLRSFQPYR
                     "
     misc_feature    4828..4843
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 25.23"
                     /tool="antismash"
     misc_feature    complement(4828..4844)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: medium, score: 28.54"
                     /tool="antismash"
     misc_feature    complement(4829..4844)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: strong, score: 31.21"
                     /tool="antismash"
     gene            5001..5288
                     /locus_tag="KEM60_RS17035"
                     /old_locus_tag="KEM60_02971"
     CDS             5001..5288
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="KEM60_RS17035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KEM60_02971"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_219108448.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.1e-13,
                     bitscore: 41.4, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKSLAATALALTILSATGATAAYADAQVDGGLWDYGHNYKDVWS
                     NYWHPSKSHGSSVIKNGQKFDSGCVGANVRSYASTGANWWDSVAEYYRFC"
     misc_feature    complement(5449..5464)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.63"
                     /tool="antismash"
     misc_feature    5879..5888
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.52"
                     /tool="antismash"
     gene            5908..7482
                     /locus_tag="KEM60_RS14405"
                     /old_locus_tag="KEM60_02972"
     CDS             5908..7482
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KEM60_RS14405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KEM60_02972"
                     /product="hypothetical protein"
                     /protein_id="WP_219108450.1"
                     /transl_table=11
                     /translation="MEKFVASQGYSSHYQDEFSLPHAIEFFLSGAKFYAFAITTLLVVA
                     LIVAGVIHQAKGLAIQQLHGHGPVQQAISLARVLSRTAGVTILVAGGLSILSLYPYNKL
                     AFFGEFYVFSVLIACLPLTAGFIVGLLTLRAIQSMDTIASIKGRLPFRTGHLIVYLIRI
                     PTIVLMLAVVHGLSAGALTISTLTSARAESLRYGATYALAMADVPHDDRAGWLRRRLQE
                     VQRDGDLRTVTYLSVLADQSQPRALLMDAALYHRLGHSETPEEKEVFVVTPPSITISDN
                     ELKKQVLQSLGGQLGGWKIRRISSTDSCFFAPITAMTGIPRGDRTHQILMVARQPLSLM
                     SPYNVASALSRGEIQFTTREAAEKMLDGPFGQLFTSITSTVDRSDQDLASVRSEYHIQW
                     INAIIVSIALVSSAFTVAMIYMARRGRRDFIRRLHGWRFSQSNLGILSFETFLGVAVAA
                     LLLQRVSSQASLLAHGEIPVPSMLDYRYTTIEVIGTLCLIAVSTLIFSLALARVSSSSL
                     RQLSSHP"
     gene            7536..8225
                     /locus_tag="KEM60_RS14410"
                     /old_locus_tag="KEM60_02973"
     CDS             7536..8225
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="KEM60_RS14410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KEM60_02973"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_219108452.1"
                     /transl_table=11
                     /translation="MDDLTKSFDQHHLWSDLNLRFEPGMRIALTGASGSGKSTLLNCLG
                     LLEQPTNGTIYWGDHNLTALNSGGRRRFRRDHLGYLFQDYALIEDATVAENLLVALNAW
                     GSRNSIKGNKRLAVAQALDQVGLSGREKDRTHILSGGEQQRVAMARILLRLPEIVLADE
                     PTAALDGENGELVLSFLDDMAHRGSIVVIATHHLDIRDRCDEVVHLPGNGDEVHVTSRI
                     AAHSINH"
     gene            complement(8309..9547)
                     /locus_tag="KEM60_RS14415"
                     /old_locus_tag="KEM60_02974"
     CDS             complement(8309..9547)
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503514.1"
                     /locus_tag="KEM60_RS14415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KEM60_02974"
                     /product="AI-2E family transporter"
                     /protein_id="WP_255574699.1"
                     /transl_table=11
                     /translation="MTDGTAGTNAVTDEENRAMTDLPADPTPPPPSAPGRSSAATQPSE
                     AATQPSEAATEKLANSLVLHMPRMFLIGVGGAGLAFCIMFVQNLGGVIAPVFLALNLMV
                     TAHPLHTWMARHGVNRGIASAITGLVVLVVLAAFFAIIGWSLTQLIVSLPSYNRQFEDL
                     GGELSHRMADVGITTEIMARQLQQISPSSVVGLITPVFTNLSTAATVITVMVAVIFFMM
                     MDSASIDYRLDLAQNAHPRLVGAMHAFAEGVRRYWIVSSIFGLISAALDVVALTLIGVP
                     LAMVWGILAFLCNYIPNVGFIISLVPPALLALLALGPGHAVMVIVAYLVMNFVVQSLIM
                     PRFTGQAVGITPTLVFVSLLFWSWVLGVLGALLAVPATLLFKAILVDADPRSRWANALI
                     ASDPMTYLDPDEK"
ORIGIN
        1 tcattgaaca tggccgagcc actccgggat atttgggaat tgctgcgacg gatgcgacgg
       61 tccgggcggg gtcggcggcg gtcagtggcg ccggggtcgc ttcggtggcg gccaacagtc
      121 cagccgcagg tgtcggtctc gtcaagggtg atgtggtcac tgcagtggac ggggttcccg
      181 tggacagtgc caccagcctg gtcgggcgga tccgggcggc gaatcccacc caacgcatca
      241 cggtgaccgt gcaccacggc gatcaagtcc gcgatgtcgc cgtcaccctg cagcctcggc
      301 cagagaagtg aactgcgcga agcgcgcctc acagccgaca cctaacgtcc tgaggggtgc
      361 acaacagcag cactgttcat cgtggtggac agcttcggga gacgtccgcc cggaggtatg
      421 tcagatccat ctgcaccgag gagtatccat gaccatccgt agacgagtga tcaccactgt
      481 ggccggggtc gctgcgggta tcgcgatcat cggcggagct gccgctgcca acgccgatac
      541 caccaccagc gctgacagca aaggcaccac gcagcagtcc gacacccagt gtcatggcgg
      601 cggagagaag caccggcgta ccccagtgac tggtgacgag gccactaaag tgggggacgc
      661 ggtcaaagcc aaggatgctg cagccagcac cgacaaagtc ttcaaacgcg ccgacggttc
      721 gtacgacgtc ctggccacga aaggcgacaa acacacccat ttccgagtca gcgctgacct
      781 gaaaactgtc acggagaaga ctcgtggcgg gcacggcaag ggtggtcacg gccagggcaa
      841 ggggcgtcct gcgcccggcg aggccggtcc aggtgaaggt ggcccacgcc ctgaaggacc
      901 ccacggcgaa ggtccgcgtg atggcgccgg gacgggcagc gcctccccgc agccgaaggc
      961 ctaccagggc tgaccccccg tcaggctgcg gtgtcgacgc ggccgaggcg gcgctgagac
     1021 agcaccaccc cgatggtggc cccgatcacg gcaggcagca tccatccgaa acctgattgc
     1081 agaccgggca gcactgtcgc caccatgtcg agcagggtgg tgacggtgct ggacccggag
     1141 atcgacgctg gcagtgcccg gagcgcatcg aagaccgcag caacgcacac gaagccgatg
     1201 gtccagcggt agacctctgg tcggtgctgc aaccagcgcg tcgcgagggc cagcaggatc
     1261 aacgcgatgg cgatggggta gaggaacatc aacaccggca cggacacctt cacgatcgcg
     1321 gcgagtccga tattggccac gcccagtgag atagccgcga agatctgcac ccaccggtca
     1381 tagggcatcc ggtccgggaa gacctgcgca aacatttccg cgcaggcggt ggtgagacca
     1441 atggcggtct tcaagcaggc aaaaaagacg attgcggcca tgaggagctg tccaccggga
     1501 ccgaaatagt gtcggctcac cgcggcgagg atcgccccgc cgttagcggc accggcagcg
     1561 acggtgaggc tggtcgcgcc cacaaaagcc aacaggccgt agagcaccga catggcgatc
     1621 ccagcgatca ctccggcctt gccggtctgg actgccactc ctttgatgtc acggacaccg
     1681 agtcggtgga tgctgtcgat gacgaggacc gcgaaggcaa gagaccccaa ggcgtccatc
     1741 gtgccgtacc catcgaggag ccctgcgacg agcggcgatg tggcgtaggc gccggtcggc
     1801 gccgcgagtg ggccgtcagt catcggggtg atcactgcga agatcagcac cgcactcaac
     1861 gaaatgagga aagcggggtt gaggtattta ccgatccagt cgatgagctt gcctgggcgc
     1921 atcgcgaaca gggcggtgag gccaaggaag atcaccgaga acacggcgag ggcgaccgtg
     1981 gccatgctat ctggcagcag cggccgcacc ccgacctcat aagacaccgt cgccgtacgg
     2041 gggatcgcaa agagcgggcc gatggtgagg taaagcgcac aggtgaagaa caccccgaat
     2101 cggcggccga cgcgggaggc gatctccagg acactggacg tgtgggagac ggccgaggcg
     2161 atgacaccaa cgatggggag cccgacagcg gtcagcagga atccgagggt ggcgatagtg
     2221 acgtcacggc cggcggcgcg cccgagctcc accgggaaaa tgaggttgcc ggcgccgaag
     2281 aacaacccaa aaagcaacga gctgaccaga atcgactgtc caccgctcag acctgatctg
     2341 ggcattcggg tggtgctcgg tgcggccgag gtgggtgacg gcgctgaact acgagtgtcg
     2401 gacacgatgt tcaagagaac acaccaaggc cctgacaccc aagtccagtg gtcggagcgg
     2461 gttagcggta gggctggaag gaccgcaacc gcaaactatt cgtcacgaca aagaccgaac
     2521 tgaaggccat cgccccgctg gcgatgatgg ggttgagcag accgaccacg gcgaggggga
     2581 tcgcagcgcc gttgtaggcg aacgcccaaa acaggttgcc tttgatggtg gccaaggtac
     2641 gccgggacag ccggatcgcg tcgactgcgg agagcaggtc accccgcacg agggtcagat
     2701 caccggcttc gatggcgacg tcgctgccgg tgcccatggc caaaccaaga tcggcttgag
     2761 ctagagcggc ggcgtcattg acaccgtcac caaccatggc gaccacccgg ccttccttct
     2821 gcaggcgagc gacatgagca actttgtcgt gcggcatgac gtcagcgatg acgtggtcac
     2881 cgtcgatgcc gatgtcccgg gcgatggcga gggcggcacg ttcgttgtcc ccggtgagca
     2941 ggatcggggt gaggcccatc gccgtacatt tcgcgatggc ttgggcgctg gtggctctga
     3001 cggtatcggt gacgacgatg atgccgcggg gagtgcccgc cgtaccgacc cacaccacgg
     3061 ttgctccgtc gcctcgggcg gcgtcagcgg cggcgaggag ttcagcgggg gcggcttctg
     3121 ccgggtcgtg gcgccccacg gtgaccacca ctgcgtcgac agtgccggtg acgcccagtc
     3181 cggtgtggct ggtgaagtcg gtgacggtgg ggaggtcccc gagggtgtcc cggccatggg
     3241 cggtgatcgc cgcagcgatg gggtgggctg atccgtgttc aacggcagcg gcgagtcgca
     3301 ggatgtgctg agggtcttca cctggtgcag cgatgacatc gcgcacgctc atcaccccgc
     3361 tggtgaccgt cccggtctta tcgaggacga ccgtgtcgac acgccgggtg gattcaagga
     3421 tctggggtcc tttgatgagg atgccaagtt gggcgccgcg gccggtgccg accatcaagg
     3481 cggtgggggt ggccaggccg agggcgcaag ggcaggcgat gacgaggacc gcaaccgcgg
     3541 cagtgaaagc ggcggtggtg ctctggccaa gcaggagcca cccagccagg gtcgccattg
     3601 agaggaggat gaccgtgggg acgaagatcg ccgcgatccg gtcggcgagg cgttgcacct
     3661 gcgctttgcc agtttgagct tcttcgacaa gccgggtgat ctgagcgagg cgggtgtcag
     3721 caccgacgcg agtggcgcgg acgtgcagga gaccgtcggt gttgaggcag gcgccagtga
     3781 cggggtcacc agtggtgacg tcgacgggga cgggttcccc ggtgagcagg gaggtgtcga
     3841 cggctgagct gcccgtcagg accaccccgt cggtggcgat cttttcacct ggccggacga
     3901 tgaattcgtc accgacagcg agcgattcca cggggatgcg gatctgggca ccgtcacgga
     3961 cgacggtgac ttctttcgcc ccgaggtcga ggagggcacg gagggcgtcg ccagagcggc
     4021 ggcgggcttg ggcttcgagg gtgcgcccga ggaggatgag gacggtcacc atcgcagcag
     4081 tctcgaagta gagatgttct gtcccgtcgg cgtgccgagg catcagctcg aacgccatcc
     4141 gcatggtggg gtctccggcg ctgctgaaga acaacgccca cagcgaccag gtccaggagg
     4201 cgaggactcc gacactgacg agggtgtcca tggtggctcc gccgtggcgc agattgaccc
     4261 aggccgcgcg gtggaaaggc catgcacccc agagcgcgac cgggctggcg aggaccagac
     4321 agagccactg ccaatagtcg aattgggctg gcgggatcat cgacaccagc aggacgggga
     4381 tgctcagcgt cgcggagacg acgaggcgtc gacgcaggtc agcggccgtg tccgcgtgat
     4441 cgtctctggt ggtcgcgtcc gtgggttgat cgctggctag tacggcgggg cgcggatcaa
     4501 tgagagtggc gtgatatccg gcggcttcga cggtgctgat caaccggtcg atgtcgatgt
     4561 catcgccgtg gtcgatgtgg gctttttcgg tggcgaggtt gaccgtggcc tggacgccgt
     4621 caattttgtt gagtttgcgt tcgacgcgcg ctgagcagga ggagcaggtc attccaccga
     4681 tgaggagttc ggtggtgtga cgctgtggtg caacctcggt catgggtggt gccttcccgc
     4741 gggctgtggg gccggtccgg ggtggggccg gggtggggcc gggtggcggc acaccagagt
     4801 ggcgggtcgc cgtacgacgt ccccacgata cccccaaggg gtatgcaggg ggcgagcgag
     4861 gtcgcaggac aacatatttc ggacgaggtc agcaagcagt caagcttggg tcaagtctct
     4921 tgactcacct caggcaaggt tcgaacatat tgaagtcgcc aaaattcgag cgacatctac
     4981 agcgaatcag cggagggatc atgcgtaaat ctttggcagc aaccgcactc gcactcacca
     5041 tactcagcgc cacaggagcg acggccgcct acgccgatgc ccaagttgac ggcggactct
     5101 gggactatgg acacaactat aaggatgtct ggtcaaacta ttggcacccg agcaagtctc
     5161 atggaagctc agtcataaag aatggccaaa aattcgactc gggttgcgtg ggcgcaaatg
     5221 ttcgctcata cgcctccaca ggcgccaact ggtgggacag tgttgcggag tattaccgct
     5281 tctgctgatc agcgaaactg agtctctcaa gcctgggcta tggctacctg gcactagccc
     5341 aggcttctaa ggttgttatt cacgaacgat tagacataga acgaccaacc cgcctgaagg
     5401 gcctgggacg tacaagtttt atgattttcc acaagaggca aattattcgt gaataacgcc
     5461 ttaaatcgaa acataaggaa taaaatgcgt cttagccgaa ccttgagatc tgcagttgtg
     5521 ctcgcgatag catttggttt cctgttctcg tttttctgtt ttaagactct ttccgagcag
     5581 agcgagatgg gggccacggg taggatcatg ccatacggtg cgaagtcgag agtctcgcca
     5641 cgtgaatact tttctagcct ccaacagtta gcatcgactg aacacattga tgtcgcagtc
     5701 atagcgcccg acccatcagg cgctcaaagt gcagaaattc tgtatatgat agggggttca
     5761 ggcccacaag ggcctgggca aaaatgggtc actggccatg cccagttcaa tccagatgcg
     5821 cacttgagta ctcagcagtt ctctgctgat accaggcttg ccccttcaag ccccctgctg
     5881 atatttgagg accgcgcacg agtccccgtg gagaagtttg tcgcttctca aggctatagc
     5941 tcgcattatc aagacgagtt cagtctccct catgccattg aattttttct ctcgggggcg
     6001 aaattttacg catttgccat caccacattg ctggtggtgg cactgatcgt cgcaggcgtg
     6061 atacatcaag ccaaagggtt ggctattcaa cagctgcatg ggcatggccc tgttcagcaa
     6121 gcaatttccc tggcacgagt cctcagtcgc actgctggcg tcaccatttt agtcgccgga
     6181 ggtctttcaa ttctctcgct ctatccgtac aataaacttg ccttttttgg tgaattctat
     6241 gttttttcag ttttgatagc ttgcctcccc ttgactgctg gattcattgt tggattatta
     6301 acgcttcgcg ccattcaatc catggacacg atagcatcca ttaaaggaag gctccctttc
     6361 cgcaccggcc acctcattgt gtatttaatc cggattccca ctatcgtgct catgctcgcg
     6421 gtggtgcatg gcttgagtgc aggtgcttta acgatatcga cgctgacctc tgcccgcgca
     6481 gagtcgctgc gatatggcgc aacttatgca cttgccatgg ctgacgtgcc tcatgacgac
     6541 cgggcgggat ggctgagacg acgtctccaa gaagtccagc gagacggtga cctgcgaact
     6601 gtcacctatc tatcggttct cgctgaccag agtcagccca gagccctcct aatggacgcc
     6661 gctctctacc accgattggg ccactccgag acgcccgagg aaaaagaagt ttttgttgtc
     6721 acaccaccga gcatcaccat cagtgataac gagctaaaaa aacaggtgct tcagtcgctt
     6781 gggggccagc tgggaggctg gaagattcgt aggatctcat ccacggacag ctgctttttt
     6841 gccccgatta cggccatgac tggcataccg cgcggagatc gcacacatca aatattgatg
     6901 gtggctcgcc agcccctctc actgatgtcc ccctacaatg tcgccagcgc tctcagtcga
     6961 ggagagattc agttcacgac gcgagaagct gcagaaaaaa tgcttgatgg tcctttcgga
     7021 caattattta cttccatcac ttcgacagta gatcgctccg atcaggacct cgcctccgtg
     7081 cgcagcgagt atcacatcca gtggattaat gccataattg tttccatcgc gcttgttagc
     7141 agcgctttta ctgtcgcgat gatttatatg gctcgacgag ggcggcgtga ttttatccgt
     7201 cgcttgcatg ggtggcgctt ctcgcagtca aatcttggga ttctgtcatt tgagaccttt
     7261 ttaggggttg cggtggctgc gctactgctt cagcgagtat ccagccaagc cagtctcctc
     7321 gcacacggcg aaattcccgt accatccatg cttgactacc gctataccac tattgaagtc
     7381 attggcactc tatgcttaat cgcggtgagc actttaatct tttctttggc attagccaga
     7441 gtcagctcat cctcactacg tcagctcagc tcacacccct aaggaagcta ccgttgagtc
     7501 cttcattcga ttccacgtca cctgcgatgc ttcgtgtgga tgacctcacc aagagctttg
     7561 atcaacatca tctttggtcg gatctgaacc tgcggttcga accaggtatg cggatcgctt
     7621 tgactggcgc gagtggcagc ggcaaaagta cgcttttaaa ctgtcttggc ttgctggagc
     7681 agcccacaaa cggcactatc tattggggcg atcataactt gacagcactg aactcaggtg
     7741 gccgtcgccg cttcagacgt gatcacctcg ggtatctctt tcaagactac gccctgatag
     7801 aggatgcgac ggtagccgaa aatctgctgg ttgcattgaa tgcctggggt tcgcgaaata
     7861 gcattaaagg caataagcgc ttagctgtag cacaggcctt ggatcaagtt ggattaagcg
     7921 gcagagaaaa agatcgcacc catattttga gcggcggaga gcaacaacgc gtagctatgg
     7981 cacggatctt gcttcgtctg cctgagattg ttcttgcaga tgagccaacg gctgccctcg
     8041 acggcgagaa cggggaactg gtgctcagct tcctcgacga catggcgcat cgcggatcga
     8101 ttgtcgtgat cgcgactcat catctcgaca ttcgtgaccg atgcgacgaa gttgttcacc
     8161 ttccagggaa cggcgatgaa gttcacgtga cgtcgcgcat cgcagctcat tcaatcaatc
     8221 actgaacacc gtgaacagta cagcgaatcg cggatgccag ctcacgccct aggggtaagg
     8281 ccgaggcttc tcttcgtccg catcgacgct acttctcgtc gggatcgaga taggtcatcg
     8341 ggtcgctagc gatcaacgca ttcgcccacc gagagcgcgg gtcggcgtcc accaggatcg
     8401 ctttgaacag caacgtcgcg gggacggcca gcaacgcacc gaggacgccg agcacccacg
     8461 accagaacag cagggagacg aaaaccaagg tcggggtgat cccgactgct tggccggtga
     8521 accgcggcat gatcaacgac tgcaccacga agttcatcac caggtacgcc acgatgacca
     8581 tcacggcgtg gcccgggccg agggccagca acgccaacag tgctggcggc accaacgaga
     8641 tgatgaaacc cacattgggg atgtagttgc acaagaaggc caagatgccc cacaccatcg
     8701 ccaacggcac gccgatcagt gtcaaggcga cgacgtctaa tgctgcggag atcaatccga
     8761 aaatggagga aacgatccag tagcgtcgta ccccttcggc gaacgcatgc atcgccccga
     8821 ccagccgcgg atgcgcattt tgggccaagt ccaagcggta gtcgatgctc gcggagtcca
     8881 tcatcatgaa gaagatgact gcgaccatga cggtgatcac cgtggcagcg gtcgacaggt
     8941 tggtgaagac cggcgtgatc agccccacca cgctggacgg actgatctgc tgcaactgtc
     9001 gagccatgat ttcagtggtg atccccacgt cggccatccg atgtgacagt tccccaccga
     9061 gatcctcaaa ttgccggttg tacgacggca aactgacgat cagttgggtc aacgaccatc
     9121 cgatgatggc gaagaacgcc gctaagacga ccagcacgac cagcccggtg atcgcggagg
     9181 cgatgccccg gttcacgcca tgccgggcca tccaggtgtg caaaggatgc gcagtgacca
     9241 tcaggttcaa cgccaggaat accggtgcaa tcaccccacc gaggttctgg acgaacatga
     9301 tgcaaaacgc caaaccagcg ccaccaacac cgatcagaaa catccggggc atgtgcaaca
     9361 ccagcgaatt cgccagtttc tcggtggcag cttcgctcgg ttgggtggca gcttcgctcg
     9421 gttgggtggc ggcggagctg cgcccaggag cagatggagg cggtggagtc ggatcggcgg
     9481 gtagatcggt catggcgcgg ttctcctcgt cggtcacggc attggtcccg gccgtcccgt
     9541 cggtcacctc ctgtccgcac cgccaaagat gaccagtacg gcgtcaggtg ggttcgcctc
     9601 ccggcgatcc gtccaccgtc ggcacacggt cgggaccgtt cgtgccgatt ctgcccgatc
     9661 tgtctgggaa gtgatgcagg agccactgag cccgtgcgcc gaaacgaccc tcagctacca
     9721 cctcggtcac gcgtcgatgt agtcagtttc gagactcagc cgggacaccc catggcttgc
     9781 ctgagcttcg atgacggccc ttgctctccc atgagagagc ctcgtttacc acacctcggg
     9841 ttttgatgtc tgcattttag tgaaagacaa cgggagacaa agactgtcgc atgccaatcg
     9901 catcccggac gggcgatcgg tgccttcccc aggctgtgaa tcatgcatcg gttgactcat
     9961 ggacacccta cgaacatgta tcttccgatg cacgaaagga atcccagatc cggtgatctg
    10021 aacatccgtc caggaatggc agttggggtc gacgggtgtc ccagccgata ccttcgggtc
    10081 agtggcactc ctctcgctgt ccgtggtcat cgagtgcccg atctgcgaga cagatgagcc
    10141 tcgggaagat cacaggtaca acacgatcct gacaagagtc tccagatctg acatctgcgg
    10201 tcgatcccac ccgacaggac aacccgggtg cgatgggtca tgtatgcgtc aggcgagtca
    10261 cagtcacggc tgaccagcgc agacgaac
//
