LOCUS       NZ_JAHPZL010000007     11398 bp    DNA     linear   CON 18-AUG-2024
DEFINITION  Austwickia sp. TVS 96-490-7B contig_7, whole genome shotgun
            sequence.
ACCESSION   NZ_JAHPZL010000007
VERSION     NZ_JAHPZL010000007.1
KEYWORDS    WGS; RefSeq.
SOURCE      Austwickia sp. TVS 96-490-7B
  ORGANISM  Austwickia sp. TVS 96-490-7B
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Dermatophilaceae; Austwickia.
REFERENCE   1  (bases 1 to 562944)
  AUTHORS   Bruer,T., Sarker,S., Lynch,S., Jack,C., Helbig,K., Isberg,S. and
            Tscharke,D.C.
  TITLE     Draft genome assembly of Dermatophilus crocodyli, causative agent of
            dermatophilosis in saltwater crocodiles and a proposed taxonomic
            reassignment to Austwickia crocodyli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 562944)
  AUTHORS   Bruer,T., Sarker,S., Lynch,S., Jack,C., Helbig,K., Isberg,S. and
            Tscharke,D.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2021) Immunology and Infectious Disease,
            Australian National University, Garran Road, 131, Canberra, ACT
            2601, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: SPAdes v. 3.13.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Reference-guided Assembly :: GCA_900187045.1; GCA_003391095.1;
            GCA_012277275.1
            Genome Coverage                   :: 115.0x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019331795.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 02:33:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,297
            CDSs (total)                      :: 3,245
            Genes (coding)                    :: 3,183
            CDSs (with protein)               :: 3,183
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 14 of 62
            Pseudo Genes (incomplete)         :: 53 of 62
            Pseudo Genes (internal stop)      :: 9 of 62
            Pseudo Genes (multiple problems)  :: 13 of 62
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:42:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 16627
            Orig. end                         :: 28025
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHPZL010000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11398
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11398
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11398
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6398](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6398)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            397..1407
                     /locus_tag="KEM60_RS08240"
                     /old_locus_tag="KEM60_01709"
     CDS             397..1407
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1033: iron
                     compound ABC transporter, periplasmic"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /locus_tag="KEM60_RS08240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KEM60_01709"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_219107063.1"
                     /transl_table=11
                     /translation="MSLNTTRKHRRAVTIALLSALALTGCGQSVSTPQASGSSSAATVT
                     LTNCGADQTYPVGRERIVALGTGQADLIARLGAAKKVVAVAQTHGEKLPGALEADGHKV
                     DIISKDAPPTKEQLLGARPDLVLSPTTYEFTAEKGYATQDQLRQSGAQVYVAAAGCFQR
                     RSTAEVTDLLTDIDALGKIIGAEKTAAELRAQAETQLKTAKDRAQGKTAPTVAQLFIEG
                     DTITAIGAGVEHSMAAAAGGTSVFSPTDEAFSKFFAAEVSRETIVAKNPQVIVFTTTGP
                     DHEKSTREWLKKHLGGVKAVHDDRLVAIPGTHVLPGTWGTIDAVTTMNKAFYPQD"
     gene            complement(1463..3205)
                     /locus_tag="KEM60_RS08245"
                     /old_locus_tag="KEM60_01710"
     CDS             complement(1463..3205)
                     /EC_number="7.-.-.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /locus_tag="KEM60_RS08245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KEM60_01710"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_219107064.1"
                     /transl_table=11
                     /translation="MNTSGTTAAPLTVTAPSGGAVSAPTRATSTITDLARLAPSLRGER
                     GPLLWTALVDLAANVSLIALSVTTAHLVATAVLYGRADEGPWWVAAVVLALLRPVLTWH
                     EMDVSHAVAYRVLAALRMALYDGYARGVPSRGGVHSGRLAATAMTDVEKLEFFYAHTVA
                     QMAAAAVLVVVGVPVAAWVSPALAGVLVAGLVLLVVTTWPLLGRGERWGATQQHRRTEL
                     SERAVDLLAGTREILVFDRREQAVADLAAAAARADAPARRLRAVEAWGAGIRESVVVLT
                     SVALMLVAAGLPGCDPVWVPCLLAGSLTFFAPIAEAVAVAATLQPHRASARRVAEGMSL
                     GADIGAALPGPGPERERVSGVAPVSAQRQQAASISTRGVRFDYPGRAPIMVPDLEIEPG
                     ELVGVAGPSGVGKSTLARLLTGLWRADAGMVLLGGRTPEQIPAGEWPSWVQLVEQDAPL
                     VHGSLAHNLRLGAPDATDGDLESALQQVGLSVTDEAFPLGLDTAVGEDGTTLSGGQRAR
                     VALARALLLEPQILVLDETTAALDAAAESAVMDVVEQLRCTVLVISHRERTLARTPRVI
                     RWLP"
     gene            complement(3202..4992)
                     /locus_tag="KEM60_RS08250"
                     /old_locus_tag="KEM60_01711"
     CDS             complement(3202..4992)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /locus_tag="KEM60_RS08250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KEM60_01711"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_219107065.1"
                     /transl_table=11
                     /translation="MSATPAHIDDVPPTTIHRWLSRIVEIRLWRLAAAHPRLLTATTVL
                     QLAVTLSYAVQAAFLAAALSGLALAARDHDTTALPTGPLSAVCLVVVTRLALAQAHIHL
                     ATRLGETVRADLRERLVRALIVPTRLHDADERLGARRSALTEGVDGVDAYVSRYLAAAL
                     QVWILCPLAIAAIAWINLWAAVAVAGCVAVAVLAPRWWKRRLTDRGRDHWDSYEQLSAD
                     HLESLRVMSTLRVLGAVGRRRQRLQERSDALHRHTVATMRISLIDTGLTDIGIQVGLTA
                     AAALAALEATGALTVPTLGVLPGAWGSGATATYLVLLLSSEAFRPVRDLARHWHAGYLG
                     LTALDSIDAALAGGTSTSDSLASAAHPPTTRSTAQSATWQDAPELTLTDVRFGYDADDL
                     VLDGIDLTVRPGGLVALTGASGSGKSTLFDLVLGLLHPQQGTIARPERVAVVSQHSYLF
                     PGTVAETLRVAAPTASEDDLWHALTDVGLADTVRGWPHGVHTVLGEGGTGVSGGQRQRL
                     AIARALLARADLLLLDEPTSALDDANARRICDVLRREAQHRIVMMIAHRPEAVAAADRR
                     LHLQGGRLCPEIIPHPQETP"
     gene            complement(5001..6398)
                     /locus_tag="KEM60_RS08255"
                     /old_locus_tag="KEM60_01712"
     CDS             complement(5001..6398)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501818.1"
                     /locus_tag="KEM60_RS08255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KEM60_01712"
                     /product="YcaO-like family protein"
                     /protein_id="WP_219107066.1"
                     /sec_met_domain="YcaO (E-value: 2.5e-45, bitscore: 150.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLTHPAPWPLDALVDEHTGLIRRVRTVLRPERAPHAYTSMTAE
                     VADARRLGEWPADRVSLGTTFGDPHGAWIAAVAEACERYCGNYLPPEGDDLRRGTAAEL
                     RADGRQVIDLDRLPRFADWQVRRPGFEYTPLDEHTPTLWVRCAEDHPEHPGRGTAGEVW
                     APSSLIHLNWRLQRYRDLPRIHHLNYAGIATGQGFADACDRALYEVIERDALELWWHLG
                     GPARGIDPATIPGLTDAMAGCDLDYWVVAMPHEYAPAFAALVHDPHTGVYAAGFSAKPD
                     PAEAARKAVLEAVHTWIYTLGTVDADGWVFHAITAGLMATGLHLDHRDDHHYLDSAGPH
                     FAAVRDLGAHVQIWQDPRTHHLARRFTHPDLGVGDLHEIEAISSAHLRRRLADAGHRVV
                     VKDLTTPDVAPTGLAVARVLVSDLVPNAPAAFAYYGMPRFADAARQRGWPIPHGPDDLE
                     LTPAPHM"
     gene            complement(6395..7420)
                     /locus_tag="KEM60_RS08260"
                     /old_locus_tag="KEM60_01713"
     CDS             complement(6395..7420)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /locus_tag="KEM60_RS08260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KEM60_01713"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_219107067.1"
                     /transl_table=11
                     /translation="MTTSTHTPLPAATTSATTVPITTVDGHRLHTLHLPATTTDTRGTV
                     LLRTPYGVEALLPEARSWAGAGYRTYLQDVRGRYRSEGHWTPYAHEGSDGADTVRALHR
                     DGGITGPLLLSGSSYGAHAAIDTARALTVTDSLSARPAGVIAMVPALGLWDTAHAPDGT
                     PRLRDRIGWWWQHGHGSISHTPLDPTTLDALTHRATHSQVTELFADHPDLDGPWRRLWS
                     APRPDPTTRWSGLHTPLLVIGGSRDFFAEDSRCLVQHWPGPAALLWGDWGHGLGTHLPA
                     THPVRQALRAHGGLLPPILTWADAAAHHQQHPDVPHPHLTARCRLDPIHGWRPLPDREE
                     P"
     CDS             complement(7417..>8159)
                     /codon_start=2
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KEM60_RS08265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KEM60_01714"
                     /product="hypothetical protein"
                     /protein_id="WP_219107068.1"
                     /transl_table=11
                     /translation="RPPARTGMSPHPAKTRDRPMTHPPLQSHGRDVLRTPDGAFMRLAP
                     EATPAQLDAALAQRARRRRPHPAVITVHGDAPQLFTPVVAALTPLTTPTGDADEPTPLA
                     VALHAVTGPADEDLADADLAAGSAVIRVHREDDVLFVHPLADPADPHAVSAHDLRARRL
                     AASPAADLLSQLWQDRGGDPLTDLPPTAAHLAVARILQHALHYLHRQPHRGPHLLHRIE
                     MTPLRVDDHPVLRVPELHTPPRPRS"
     gene            complement(7417..>8161)
                     /locus_tag="KEM60_RS08265"
                     /old_locus_tag="KEM60_01714"
     assembly_gap    8162..8261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="align xgenus"
     gene            <8262..9182
                     /locus_tag="KEM60_RS08270"
                     /old_locus_tag="KEM60_01715"
     CDS             <8262..9182
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KEM60_RS08270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KEM60_01715"
                     /product="ABC transporter permease"
                     /protein_id="WP_219107069.1"
                     /transl_table=11
                     /translation="DTPRNILTLSQRQPATTHQHSPSPGTTHHPKRGTDPFTPRVETGD
                     LLVGTPMVLDSSHDVERLIGHPLSKDQVLAFNKGGLLVPDSHKSIQLKSLKSLPTKDHQ
                     GRDTGVLHITHIQDVPDAYAQYLGFISSGKARQAGWRLDQAFTVYDNVSPIQISDALKA
                     PDKKGFDPNYVQIYKVPEKITVPETLVWSATGTGVLFVLLTCWVAASHTKALREYRNSL
                     IAIGLSHRLLFGITLTVLLFVVAIPAFVGLGVALLANHLAWGMILESGRSAFIPWEWIM
                     NGMLMTALSITFGTLVGAFMLRRQS"
     gene            complement(9250..10062)
                     /locus_tag="KEM60_RS08275"
                     /old_locus_tag="KEM60_01716"
     CDS             complement(9250..10062)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780106.1"
                     /locus_tag="KEM60_RS08275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KEM60_01716"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_219107070.1"
                     /transl_table=11
                     /translation="MSPTLPHPPHTPWQRYVAIGDSFTEGMCDTHPDHDDEYIGWADRL
                     AALLHQVAAREGHQLDYANLAVRGRLLTDITDRQLPAALALEPDLVSLVGGGNDILRPN
                     ADMDALAHQLDHAVRTIRATGADVILATSTDTRGAPLLRHVRQRHALHTANIWTIARTH
                     GCHVIDQWGMRPLQDLRMWAPDRIHMTPEGHRRVALAAFAALGYTPDDPDWDVPLPAAA
                     PETWTTQLRADREWARVHVGPWVHRRLTGRSSGDTRTAKRPQATPYQD"
     gene            complement(10059..10748)
                     /locus_tag="KEM60_RS08280"
                     /old_locus_tag="KEM60_01717"
     CDS             complement(10059..10748)
                     /EC_number="3.2.2.27"
                     /GO_function="GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502671.1"
                     /locus_tag="KEM60_RS08280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KEM60_01717"
                     /product="uracil-DNA glycosylase"
                     /protein_id="WP_219107071.1"
                     /transl_table=11
                     /translation="MSTPRPLHHLIHPTWAQALEPVTEQIHTMGDFLRAELAAGRGYLP
                     AGEHVLRAFTRPLPEVRILIVGQDPYPTPGHAVGLSFSVAPDVRPLPRSLVNIFQELTT
                     DLGVPPPSCGDLTPWTERGVLLLNRSLTVRPGAAGSHRGKGWEAVTDRAITVLAERAAA
                     GQPLVAILWGRDARNLAPALPGVPRIESPHPSPLSAHSGFFGSRPFSRANHLLTQLGAS
                     PIDWRLP"
ORIGIN
        1 caccttcgcg tccctctccg gcggcgaaaa acaacgcatc ctgctagctc gcgccctcac
       61 ccagcaagca cccgtcatga tcctcgacga acccactaac catgctgacc tcgggtttca
      121 acacgacctc ctgcacctgg tcacgacccg aggcgccacc gtgctagcag ccctccatga
      181 catcaacttg gccctggcct actgcgatca agcggccgtc atgcaccacg gacagctgca
      241 cgcccacgga cccgtcaccg acgtcctgac cccacaactc ctcgacgaca cgctcggcgt
      301 gtcctcgatg gccatccccg acccccgcgg tggacaggtg ctgtccttcc gccgccgcac
      361 cacccacctg tcgaccacca cgaccaagga caacccatga gcctcaacac caccagaaaa
      421 caccgccgcg ccgtcaccat cgcactcctc agcgcgctcg cactcaccgg ctgcggtcag
      481 agtgtcagca ccccacaagc aagcggcagc agcagcgccg ccacggtcac cctcaccaac
      541 tgcggcgctg accagaccta ccccgtcggt cgggaacgca tcgtcgccct cggcaccggc
      601 caagcagacc tcatagcccg cctcggcgcc gccaagaagg tcgtcgcagt cgcccagacc
      661 cacggcgaga aactccccgg cgctctcgaa gccgacggcc ataaagtcga catcatcagc
      721 aaagacgccc cgcccaccaa ggaacagctg ttgggggcgc gccccgacct ggtcttgtcc
      781 cccaccacct acgaattcac cgccgaaaag ggctacgcca cccaagacca gctccgtcag
      841 tccggcgcgc aggtgtacgt cgccgccgcc ggatgcttcc agcgccgctc caccgccgaa
      901 gtcaccgacc tgctcaccga catcgacgcc ctcggcaaga tcatcggtgc cgaaaaaacc
      961 gcagccgagc tacgcgccca ggccgaaact cagctcaaaa ccgccaaaga ccgcgcccag
     1021 ggcaaaaccg ctccgaccgt cgcccaactc ttcatcgaag gcgacaccat caccgcaatc
     1081 ggcgcaggcg tcgaacacag catggctgcg gcagcaggcg gcaccagcgt cttctccccc
     1141 acagacgaag ccttctcgaa attctttgcc gccgaagtct cccgcgaaac catcgtcgcc
     1201 aagaaccctc aggtcatcgt gttcaccacc actggtcccg accacgaaaa aagcacccgt
     1261 gaatggctga agaaacacct cggcggcgtc aaagcggtcc acgatgaccg gctcgtcgcg
     1321 atccccggca cccacgtcct ccccggcacc tggggcacca tcgacgccgt caccaccatg
     1381 aacaaggcgt tctacccgca ggactgaacc agctcggggg cctgagagcc gacccctgct
     1441 cagctctcag gcccccgtca cctcagggaa gccaccggat cacccgaggc gttcgcgcca
     1501 acgtccgctc ccgatggctg atcaccagca ccgtgcaacg cagctgctcc accacatcca
     1561 tcaccgctga ctcggcagcc gcgtccaaag ccgctgtcgt ctcatccagc accaagatct
     1621 gtggctccag caacagcgcc cgcgccaacg ccacgcgagc ccgctggcca ccagacaacg
     1681 tcgtaccgtc ctccccgaca gcagtgtcca agcccagcgg gaacgcctca tcggtcaccg
     1741 acagccccac ctgctgcaac gcgctctcca gatccccatc ggtggcatcc ggtgccccca
     1801 aacgcagatt gtgcgcgagc gaaccatgca ccaaaggcgc gtcttgctcg accagctgca
     1861 cccacgacgg ccactctccc gcggggatct gctccggcgt acgaccccca agcagcacca
     1921 tcccagcgtc agcccgccac aacccggtca gcaatcgagc caaggtcgac ttgccgaccc
     1981 cggatggccc ggcaaccccg accagttcac ccggctcaat ttctaggtca ggcaccatga
     2041 tcggcgcccg acctggatag tcaaagcgca ccccccgagt gctgatcgac gccgcctgct
     2101 gccgctgcgc agacaccggc gccaccccag acacccgctc ccgctccggc cccggccccg
     2161 gcaaggctgc accgatatcc gcgcccaaag acattccctc cgcgacccga cgagcactcg
     2221 cccgatgagg ttgcaaagtc gctgccaccg ccaccgcctc agcgatcgga gcaaaaaacg
     2281 tcaacgagcc ggccaaaaga catggcaccc agaccggatc acaaccaggc aaaccagcag
     2341 cgaccagcat caacgccacc gacgtcaaca ccaccaccga ctcgcggatc ccggcgcccc
     2401 aggcctccac cgcccgcaat cgacgcgccg gagcatccgc ccgagctgcc gccgccgcca
     2461 gatcggcgac cgcctgctca cgacggtcaa acaccaagat ctcccgggtg ccggcaagca
     2521 gatccactgc acgttcagac agctcagtac gccgatgctg ctgggtggcg ccccaccgct
     2581 caccgcggcc tagcaacggc caggtcgtca ccaccagcaa caccagaccc gccaccaaga
     2641 cccccgcgag cgctggtgac acccaggcgg cgaccggcac tccgacgacg accagcaccg
     2701 cggcggcagc catttgggcg acggtgtggg cgtagaaaaa ctccagtttc tccacgtcag
     2761 tcatcgcagt cgccgccagt cgtcccgaat gcaccccgcc ccgagaaggc acgccacggg
     2821 cgtatccgtc gtacagggcc atccgcaacg cagctagcac ccgataggcc accgcatggg
     2881 acacatccat ttcgtgccag gtcagcacgg ggcgaagcaa cgccaggacg acggccgcga
     2941 cccaccaggg cccctcatcg gcccggccat agagcaccgc agtggcgacc agatgggcgg
     3001 tggtcacgga cagtgcgatt aaggacacat tggcagcgag gtccaccagg gctgtccaca
     3061 gcaatggtcc acgctcaccg cgaagtgacg gagccagtcg agcgaggtcg gtgatcgtag
     3121 aggtagcgcg agtcggagcc gatacggcac caccggaggg cgctgtcacg gtcagcggtg
     3181 cggccgtcgt accggaggta ttcatggcgt ctcctgaggg tgcgggatga tctcgggaca
     3241 caaccggccg ccctgcaagt gcagtcgtcg atcggcggcg gcgacagctt caggacggtg
     3301 cgcgatcatc atcacgatgc gatgctgtgc ctcccgacgc agtacgtcac agatgcggcg
     3361 ggcgttggca tcgtcgaggg cgctggtcgg ttcgtcgagg agcagcaggt ccgcgcgagc
     3421 caggagcgcc cgggcgatag ccaatcgttg gcgttgcccc ccggacaccc cggtgccgcc
     3481 ttcaccgagg accgtgtgca cgccgtgcgg ccacccccgc accgtgtcgg ccaggccgac
     3541 gtcggtgagg gcgtgccaga ggtcgtcttc actggcagta ggggcagcga cccgcagcgt
     3601 ctcagcgacg gtgccgggga acagatagga gtgttgcgag acgacggcga cgcgttcggg
     3661 ccgtgcgatg gtgccctgct gggggtggag cagccccagg acgaggtcaa agagcgttga
     3721 cttgcctgac ccggaggctc cggtcagggc aacgagccca ccgggccgca ccgtgagatc
     3781 gatgccgtcg aggaccaggt cgtcggcgtc atatccgaaa cggacgtcag tcaacgtcaa
     3841 ctccggtgcg tcctgccagg tggcgctctg cgccgtactc cgggtggtcg gcgggtgggc
     3901 ggcggaagcc agcgagtcgg acgtgctggt ccccccggcc agggcagcgt caatgctgtc
     3961 gagggcggtc agtccgagat agccggcatg ccagtgccgg gccaggtcgc gcaccggccg
     4021 gaacgcctca gaggagagca acagcaccag gtacgtcgca gtggcaccgg aaccccacgc
     4081 cccggggagg acaccgagcg tggggacagt gagcgcgccg gtcgcttcga gggcggcgag
     4141 cgcggcggcg gcagtcaatc cgacttggat gccgatgtcg gtcaggccgg tgtcgatcaa
     4201 ggagatacgc atggtggcga cggtgtgccg atgcaaggcg tcagaccgct cttggagtcg
     4261 ttggcggcga cgaccgactg caccgaggac gcgcagggtc gacatgactc ggagcgattc
     4321 gaggtggtct gcgctgagtt gttcgtagct gtcccagtgg tcgcgtccac ggtcggtgag
     4381 tcggcgcttc caccagcggg gcgcgaggac cgcgacggcc acgcagccgg ccacggccac
     4441 cgctgcccac aggttgatcc aggcgatggc cgcgatcgcc agcgggcaga ggatccacac
     4501 ctgcagtgcg gcagcgaggt atctgctgac gtaggcgtcg acaccgtcga ctccttcggt
     4561 gagggcggag cggcgcgccc cgagccgttc gtcggcgtca tggagtcgag tggggacgat
     4621 caacgcgcgt accaggcgtt ctcgcaggtc agctcggact gtttcgccga gcctggtggc
     4681 gaggtggatg tgggcttggg cgagagcgag gcgggtcacg acgacgagac acaccgcgct
     4741 cagtggtccg gtcggcagcg cggtggtgtc gtggtcgcgg gcagcgaggg cgagaccaga
     4801 gagcgcagca gcgaggaaag ctgcttggac ggcgtaactg agggtgacgg cgagttgcag
     4861 gacggtggtc gcggtcagga gccgagggtg ggcagcggcg agtcgccaga gtcggatctc
     4921 cacgatccgg gagagccagc ggtggatggt ggtgggaggt acgtcgtcga tgtgggccgg
     4981 ggtcgcgctc actggtcctc ctacatatgt ggagccgggg tgagctccag gtcgtcaggg
     5041 ccgtgtggga tgggccagcc gcgctgacgg gcggcgtcgg cgaagcgggg catcccgtaa
     5101 taggcgaatg ccgcaggagc attggggacc aggtcgctga ccagcacgcg agctacggcc
     5161 agtccggtcg gagcgacgtc gggggtggtg aggtctttga cgacgacgcg gtggccggcg
     5221 tcggcgaggc ggcggcggag gtgagctgag gagatcgcct ctatctcgtg gagatcgccg
     5281 acacccaagt cggggtgggt gaagcggcgg gcgagatggt gggtgcgggg gtcctgccag
     5341 atctgcacgt gggcgccaag gtcgcgcact gccgcgaagt gcggtcctgc cgagtcgaga
     5401 tagtgatggt cgtcgcggtg gtcgaggtgc aggccggtgg ccatcagacc ggcagtgatg
     5461 gcgtgaaaca cccagccgtc ggcgtcgaca gtgcccaaag tgtagatcca ggtgtgcacc
     5521 gcttcgagga ctgctttgcg ggcggcttcg gcagggtctg gtttcgcgga aaacccggcg
     5581 gcatataccc cggtatgggg gtcgtggacc agcgctgcga acgccggggc gtattcgtga
     5641 ggcatcgcca cgacccaata gtcaaggtcg cagcctgcca tggcgtcagt cagaccgggg
     5701 atggtggcgg ggtcgatgcc tcgggcgggc ccgccaaggt gccaccacag ttcgagggcg
     5761 tcgcgttcga tcacttcgta gagggctcga tcgcaggcat cggcgaaacc ttgaccggtg
     5821 gcgatgccgg cgtagttgag gtgatgaatg cggggcaggt cgcggtaacg ctggagacgc
     5881 cagttgaggt ggatgaggga tgagggtgcc cacacctcac cggcagtacc acgtccggga
     5941 tgctcggggt ggtcttcggc gcagcgcacc cacagggtgg gggtgtgctc atcaaggggg
     6001 gtgtattcga aaccggggcg tcgtacctgc cagtcagcga agcgcgggag ccgatcgaga
     6061 tcgatcacct ggcgaccgtc agcgcgtagc tctgcggcgg tgccacgacg caggtcatcc
     6121 ccttcggggg gaagatagtt gccgcagtag cgttcgcagg cttcggcgac ggcggcgatc
     6181 caggcgccgt gggggtcgcc gaaggtggtg ccgagggaga cccggtcggc gggccattcg
     6241 ccgaggcggc gagcgtcagc aacttcagcg gtcatcgagg tgtaggcgtg cggtgcgcgc
     6301 tctgggcgca ggacggtgcg aacacgccgg atgagtccgg tgtgttcgtc gacgagggcg
     6361 tccagcggcc agggcgcagg gtgcgtcagg gtggtcatgg ttcctcccgg tcaggcagtg
     6421 ggcgccaccc gtggatcggg tcgaggcggc agcgggcagt caggtgcgga tgaggtacgt
     6481 cgggatgctg ttgatggtga gcggcggcat cagcccaggt gaggatcggc gggagcagac
     6541 cgccgtgggc gcgcaaggct tgccggaccg ggtgagtcgc tggcaggtgg gtgccgagcc
     6601 cgtgacccca gtctccccag agcagtgccg ctggcccggg ccagtgctgc acgagacagc
     6661 gggaatcttc agcgaagaag tctcgactgc ccccgatcac gaggagtggg gtgtgcaggc
     6721 cggaccagcg agtggtgggg tcggggcggg gcgcgctcca gaggcggcgc cagggaccgt
     6781 cgaggtctgg atggtccgca aacagctcgg tgacctggct gtgggtagcc cggtgagtca
     6841 gggcgtccaa ggtggtcgga tcgagggggg tgtgggagat ggatccgtgg ccgtgctgcc
     6901 accaccagcc gatgcggtcc cgaagtcggg gggtgccatc cggagcgtgc gcagtgtccc
     6961 agagccccag agcggggacc atcgcgatga cacctgcggg gcgtgcactg agagagtcgg
     7021 tgacggtcaa ggcccgtgcc gtgtcgatgg cggcgtgcgc gccgtacgag gagccgctga
     7081 gcaggagcgg accggtgata ccgccatcgc ggtggagggc acggaccgtg tctgccccat
     7141 cgctgccttc gtgggcgtac ggggtccaat ggccctcaga gcggtagcgg ccgcgcacgt
     7201 cctgtaggta ggtgcgatat ccggcgccag cccaggaacg agcctccggg agaagcgctt
     7261 cgacgccgta cggagtgcgc agcaaaacgg tgccgcgggt gtcggtagtg gtggcgggta
     7321 ggtgcagggt gtgcagtcga tgcccgtcaa cggtggtgat gggcacggtg gtggcggagg
     7381 tcgtggcggc aggcagcggg gtgtgggtgg aggtggtcat gagcggggtc ggggtggagt
     7441 gtgcagctca gggacgcgta agacggggtg atcatcgact cgcaacggcg tcatctcgat
     7501 gcggtggagc aggtggggtc cccgatgtgg ttgccggtgc agatagtgca gggcgtgctg
     7561 caggatgcgc gcaacggcca ggtgggctgc cgtgggaggc aggtcggtga gagggtcacc
     7621 gccacggtcc tgccagagtt gactgagcag gtcagcggca gggctggcag cgagtcggcg
     7681 ggcgcgcagg tcgtgggcgc tgaccgcgtg tgggtcggct gggtcggcga gcggatggac
     7741 gaaaaggacg tcatcttccc ggtggacgcg gatgacggcg cttccggcgg ccagatcggc
     7801 atcagccagg tcttcgtcgg cgggcccggt gacggcgtga agagccactg ccaacggagt
     7861 gggttcgtca gcgtcgcccg tgggggttgt cagcggagtg agcgcagcca cgacgggggt
     7921 gaacagttgc ggagcgtcgc cgtgcaccgt gatgacagca ggatggggtc gtcggcggcg
     7981 agcgcgttgt gccagggcgg catcgagttg cgcgggggtg gcctcgggtg cgaggcgcat
     8041 gaaagcgccg tcgggggtgc gcaggacgtc acgtccgtgg gactgcagtg gcgggtgggt
     8101 catgggccgg tccctggtct tggcggggtg gggtgacatg ccggtgcggg ccggtggacg
     8161 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngataccccg cgaaatatcc
     8281 tcacgctcag ccaacgtcaa cccgcgacca cgcatcaaca ctctccctca cctggaacca
     8341 cacatcatcc caagcgaggc actgacccct ttaccccaag ggttgagacc ggtgatcttc
     8401 tggtgggcac tccgatggtg ctagattcat cccatgatgt tgagaggttg ataggtcacc
     8461 cccttagcaa ggatcaagtc cttgctttta ataaaggcgg cttgcttgtt cctgactcac
     8521 ataagtcaat tcagttgaaa agtctgaagt cattgccaac gaaagatcat caaggtcggg
     8581 ataccggcgt gcttcatatt actcatattc aggatgtccc agatgcttac gctcaatact
     8641 tgggatttat atcgtcaggt aaggcacggc aagctggttg gcgtctcgat caagctttca
     8701 cagtatatga caatgtgagt cctattcaaa tctccgacgc tttaaaagct cctgataaaa
     8761 aaggatttga tccaaactat gttcagattt ataaggtgcc tgagaaaatt acagttccag
     8821 agactcttgt ttggtcagcg acaggtacag gagttctgtt tgtcctgcta acatgctggg
     8881 tcgcagcttc ccacacaaaa gcacttcgtg aatataggaa ctcacttatt gcgattgggc
     8941 taagccaccg acttttattt gggattactt tgacggtgtt gttattcgtt gtagcaattc
     9001 ccgcctttgt tggactggga gtggctttgc tcgcaaatca tttggcttgg ggtatgattc
     9061 ttgagtctgg aagaagtgca tttattccat gggagtggat tatgaatgga atgctgatga
     9121 ctgctctaag tattacgttc ggcaccttgg ttggagcttt catgcttcgc aggcagtcct
     9181 gattgcgttg tattttttgc ctacgccatg agaggtggat gttactcacc cttctcgcgt
     9241 ttcagcgcgt cagtcctggt agggcgtggc ctgggggcgt ttcgcggtgc gggtgtcgcc
     9301 agagctgcgg ccggtcaacc ggcggtgcac ccacggcccg acgtggaccc gagcccactc
     9361 gcggtcggcg cgtaactggg tagtccacgt ttcgggtgcg gccgcaggga ggggaacgtc
     9421 ccagtcagga tcgtccgggg tgtagcccaa cgcggcgaag gcagcgagcg cgacgcggcg
     9481 gtgtccttcg ggggtcatgt ggatgcggtc tggtgcccac atgcgcagat cttgtagtgg
     9541 tcgcatgccc cattggtcta tgacgtgaca gccgtgggtg cgggcgatgg tccagatgtt
     9601 cgcggtgtgc agggcgtggc gttggcggac gtggcgcagc aggggcgcgc ctcgggtgtc
     9661 ggtggaggtg gcgaggatga cgtcggcgcc ggtggcgcgg atggtgcgga cggcgtggtc
     9721 gagttggtgg gcgagtgcgt ccatgtcggc gttgggtcgc aggatgtcgt tgccgccgcc
     9781 gacgagggat accaggtcgg gttcgagggc gagcgcggcg gggagttggc ggtcggtgat
     9841 atcggtgagg aggcggccgc gtacggcgag gttggcgtag tcgagctggt gtccttcgcg
     9901 ggcggcgacc tggtgcagca gggcggcgag tcggtcggcc cagccgatgt attcgtcgtc
     9961 gtggtcgggg tgggtgtcgc acatgccttc ggtgaaggag tcgccgatgg cgacgtaccg
    10021 ctgccagggg gtgtgcggtg ggtgcggcag cgtggggctc atggcagcct ccaatcgatg
    10081 ggggacgcgc cgagctgggt cagcaagtgg ttggcgcggc tgaacgggcg ggaaccaaaa
    10141 aatcccgaat gcgccgacag cggtgagggg tggggcgatt cgatgcgggg tacgccggga
    10201 agcgcggggg ctaggttgcg ggcgtctcgg ccccacagga tcgctacgag gggttgcccg
    10261 gcggcggcgc gttcggcgag gacggtgatg gctcgatcgg tcactgcttc ccatcctttg
    10321 ccgcggtggc tgccggcggc gccggggcgt acggtgaggg atcggttgag gagcaggaca
    10381 ccgcgttcgg tccagggggt gaggtcaccg cagctgggcg ggggcacgcc gaggtcggtc
    10441 gtgagttctt ggaagatgtt gaccaggctg cgcggcagcg gccggacatc gggggcgacg
    10501 ctgaaggaca gtccaacggc gtgtccggga gtgggataag gatcttgacc gacgatgagg
    10561 atgcgcactt cgggcagggg gcgggtgaag gcgcgcagca cgtgctcgcc agcggggagg
    10621 tagccgcggc cggcagcgag ctcggcgcga agaaagtctc ccatcgtgtg gatctgttcg
    10681 gtgacgggtt cgagggcctg cgcccaggtg ggatggatca ggtggtgcaa ggggcgaggc
    10741 gtggacaccc gcccacccta acggtcgtgg cggctccgac tcagggtggg atctgatccc
    10801 actcagggag gaatccggtt gttcccctgg tgcgctccgt ggttcgggtt cttacagtgg
    10861 gtagcagtgc gtcaggggcg ggttcgggtc gtccggggtg gcgccgggcc gacggttcat
    10921 cagagcgtcg gatgtccacg acagcaggga tcatcatggc acttttggaa cgtcgtactg
    10981 agggatcggg cgggccgggc acggtcgccg cttcagcgcg ggacctggtg aaggtctatg
    11041 gcacggggtc gactcaggtc gtcgcattgg agaaagtcga cattgacatt tatggcgggg
    11101 aattcactgc ggtgatgggc ccgtcagggt cggggaagtc gacgttgatg cactgcatgg
    11161 ccgggttgga ccggatcacg cagggggagg tcactgtcgg tggaaccccg ttgggccggt
    11221 tgtcggacaa ggagctcacc gcgttacgtc gggacaagat cggttttatc ttccaggcgt
    11281 tcaatttgat tccgacgttg acggcgcagg agaacatttt gttgccgttg tctattgctg
    11341 gtcgtcaacc tcaggaggac tggtatcgca cggtgattga gaccacgggc ctggccga
//
