LOCUS       NZ_CP024972            10390 bp    DNA     circular CON 18-AUG-2024
DEFINITION  Amycolatopsis sp. TNS106 chromosome, complete genome.
ACCESSION   NZ_CP024972
VERSION     NZ_CP024972.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. TNS106
  ORGANISM  Amycolatopsis sp. TNS106
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 8806031)
  AUTHORS   Liu,K. and Tao,M.
  TITLE     Discovery of new natural product by Amycolatopsis speibonae genome
            mining
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8806031)
  AUTHORS   Liu,K. and Tao,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, Shanghai
            200030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP Assembly v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 116.0x
            Sequencing Technology             :: Illumina HiSeq 4000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019330265.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 03:49:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,321
            CDSs (total)                      :: 8,231
            Genes (coding)                    :: 8,179
            CDSs (with protein)               :: 8,179
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 20 of 52
            Pseudo Genes (incomplete)         :: 35 of 52
            Pseudo Genes (internal stop)      :: 4 of 52
            Pseudo Genes (multiple problems)  :: 6 of 52
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:02:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7138074
            Orig. end                         :: 7148464
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024972.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="31"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5390](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5390
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(237..251)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.74"
                     /tool="antismash"
     misc_feature    238..252
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.02"
                     /tool="antismash"
     misc_feature    462..482
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.8"
                     /tool="antismash"
     misc_feature    complement(770..788)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.16"
                     /tool="antismash"
     misc_feature    2405..2416
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     gene            3189..3680
                     /gene="ectA"
                     /locus_tag="CVV72_RS32915"
                     /old_locus_tag="CVV72_32775"
     CDS             3189..3680
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033456.1"
                     /locus_tag="CVV72_RS32915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_32775"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_219147807.1"
                     /transl_table=11
                     /translation="MSGKHLIENPTKADGAALWRIARDSKKLDLNTPYAYLLWCHDFAD
                     TSVVAKVDGTPVGFVIGYRKQDTGFVWQVAVDASQRGKGLAGALLDELFGRLTAQGVRY
                     LETTITPDNEASIRLFASFAKRWDATVERQDLFSATDFPAEEGTHEQEDLYRIGPLSSQ
                     "
     gene            3719..4969
                     /gene="ectB"
                     /locus_tag="CVV72_RS32920"
                     /old_locus_tag="CVV72_32780"
     CDS             3719..4969
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 4.9e-73.
                     Score: 238.0. Matches aSDomain:
                     nrpspksdomains_CVV72_RS32920_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033455.1"
                     /locus_tag="CVV72_RS32920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_32780"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_219147809.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.9e-75, bitscore:
                     251.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFEELESEVRSYSRGWPVVFDRAQGSYLYDEDGKAYLDFFAGA
                     GALNYGHNNPALKRALIDYIARDGVTHALDMFTVAKRDFLQTFQEKILKPRELDYKVVF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHA
                     TPMPYDNYFDGSIPDFLYFEKLLEDSGSGLNEPAAVIVEGVQGEGGINAARVEWLKALD
                     DLCKKHGILLILDDVQMGCGRTGPFFSFEDAGIKPDIVCLSKSIGGYGIPMALTLIRPD
                     LDVWEPGEHNGTFRGISPAFVTATEALRVYWSDDELEKSTKAKGERIGAAFQGIVEAYP
                     DANLFAKGRGLARGIEFANGDLAGKVCAAAFERGLLMETSGPDGEVMKVLPPLTLTDDE
                     LTKGLSIIDEAVATVLN"
     aSDomain        3782..4792
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CVV72_RS32920_Aminotran_3.1"
                     /evalue="4.90E-73"
                     /label="CVV72_RS32920_Aminotran_3.1"
                     /locus_tag="CVV72_RS32920"
                     /protein_end="358"
                     /protein_start="21"
                     /score="238.0"
                     /tool="antismash"
                     /translation="FDRAQGSYLYDEDGKAYLDFFAGAGALNYGHNNPALKRALIDYIA
                     RDGVTHALDMFTVAKRDFLQTFQEKILKPRELDYKVVFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHATPMPYDNYFDGSIPDFLYFEK
                     LLEDSGSGLNEPAAVIVEGVQGEGGINAARVEWLKALDDLCKKHGILLILDDVQMGCGR
                     TGPFFSFEDAGIKPDIVCLSKSIGGYGIPMALTLIRPDLDVWEPGEHNGTFRGISPAFV
                     TATEALRVYWSDDELEKSTKAKGERIGAAFQGIVEAYPDANLFAKGRGLARGIEFA"
     gene            5001..5390
                     /locus_tag="CVV72_RS32925"
                     /old_locus_tag="CVV72_32785"
     CDS             5001..5390
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165143.1"
                     /locus_tag="CVV72_RS32925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_32785"
                     /product="ectoine synthase"
                     /protein_id="WP_007033454.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.1e-53, bitscore:
                     179.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVRTLDEVTDTDADIKTPNWRSKRIVLAKEGVGFSVHETTLYAG
                     TVNDFWYANHIEAVFITSGEGELENTATGEVFQLKPGTLYLLNDHDKHQVRPKTEIKCV
                     CVFNPPITGREVHDENGVYPLVTEP"
     gene            5415..6314
                     /gene="thpD"
                     /locus_tag="CVV72_RS32930"
                     /old_locus_tag="CVV72_32790"
     CDS             5415..6314
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631927.1"
                     /locus_tag="CVV72_RS32930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_32790"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_219147810.1"
                     /sec_met_domain="PhyH (E-value: 1e-31, bitscore: 109.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTDTRVDDSYPTRITGKPEHLPRTHPTVWGAEADGPMDAATLA
                     NHETRGYTVVDQLLSPGEVQAYWQELVRMSSGDHSGDERVITEAKTGEVRSIFDVHETS
                     ELIAELVRDPRVLDRARQILGSEVYIHQSRVNYMPGFKGTGFYWHSDFETWHAEDGMPA
                     PRAVSCSIALTDNYPFNGGLMVMPGSQRTFVQCAGETPENNYKSSLKEQRVGVPSEEDI
                     TALAAEYGIDQFTGQAGSALWFDSNVMHGSSNNITPYPRSNIFVVFNSVENALREPFAA
                     IEPRPAFIAGRDSTPLSR"
     gene            6868..9657
                     /locus_tag="CVV72_RS32935"
                     /old_locus_tag="CVV72_32795"
     CDS             6868..9657
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631926.1"
                     /locus_tag="CVV72_RS32935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_32795"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_219147811.1"
                     /transl_table=11
                     /translation="MGESGSPARFQLLGPVRLLDGERPVPVGGPGVRGLLALLALKVNK
                     VVALDEIIDALWGHDPPATARTIVHGNVSHLRRVLRGIQGDHPHGARILTAPPGYQLTV
                     EPERIDVHRARALLERASAEEPEKAAELLTEALALWQGPALAGVPGSVRAPELEDLRLA
                     VHGARVDADLELGRHAELIVELSPLVRANPLAERTAGQLMRALYHAGRRGDALELYRTV
                     SRATLRTLGVEPGADLRWLHERVLNDDLPVKVAAEPKAEAAPVQQLPPAVPNLAGREAE
                     LAWLDELAERAEAGETAVGVVTGTAGIGKSSLVVWWAHRAGRRFPDGILFASLRGFDPH
                     HPPLEPADLLTQFLLGLGVPAEGVPEQVHERVALYRSMIAGRRMLVILDNARSAEQVRP
                     LLPPGSRSMTLVTSRSRLDGLAVSNAAKLRVLGTLAPGDAVRLIEELAGPAGFDLNHAL
                     ARLCGYLPLALRIAGARLAASAQWSAQDLVDELGNERTRLAALDVEGPDDGVRAAFDVS
                     FRGLPPEVANTFLRLGVVQCVSAGSHLTAAIGGITVAEARRHLRVLTAHNLLAETGRDS
                     FVPHDLVRLFLRELAENELDEADRDDVLSRSVRFYQAVADRARRKMLRIVDPLDFTGAL
                     TDAQTPPIGSFDEAQDWFTAEWENLIEVLEAARAAGRHDDVWRLARVAHTYRAVYPLLD
                     EWTRLVEIGLEAAERSGDVLGRCWMLISRCAIALTFELPQGCLADAERALELAAAIGDD
                     RLIVSANIHLGSALTLLGRHDEAIRTLRRAVEETERTGDLELRGQALNNCAEAEKRAGR
                     FIEAIGHQVASLEIDRTLGDESYVVVSLNNLAELSMRIGELAAAERYVWEAVDLTISRR
                     FVLQEGVLRLTLGRVLRAGSDVDGAREQFALALKILSDVNPKLATLVRAELTDLDESP"
     misc_feature    10205..10220
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: strong, score: 24.95"
                     /tool="antismash"
     misc_feature    complement(10302..10315)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.99"
                     /tool="antismash"
ORIGIN
        1 cgcggggagc ccgtggccgc aggagatccg gctggcgatg accatggtgg gcggggcgag
       61 cctcgcggtc tggatgggcg gggtcgccac cgagacctcg catctgcttc aggcgtcgag
      121 aacgccggaa tcgaaaagcc cgtaccgggc actgctcgat ctgctcaacg ccaccgtctc
      181 gctcgacgtg ctcaccggca ccagcgcggg cggtatcaac gcggcgtgcc tcgggctggc
      241 cgaggcgttc cggtcgagcc cgcaggtgct gcgggacacg tggatcagca cgggatcgct
      301 cgacaatctg atccgcgacc ccggcgagaa ggaaccgcgg tcgctgctcg acggcgacaa
      361 ggtgctgctc ggcgatctca aggacgcgtt gcaccggatc accgacaagg cgaccgtgaa
      421 acccgactgt cccgacatca cggtgctgct caccggcacg atgatcgacg gcgagacgac
      481 caggttcgac gacgcgctcg gcaacctggt gcgggacacc gaacaccggc tcctgttccg
      541 cttcgatggt cctttgtgga cggatgatgt ggtcgggccg ctcgcgctcg ccgcgaggtc
      601 caccgcgtcg ttcccgggcg ccttcgaact ttcccggatg ccgatcggcg agaagaccgg
      661 accgctgcat ccggacatga cgaagtacac cgacgtcacg cggtcgcatt ggctcaccga
      721 cggcggggtg ctgctgaaca aaccgttgcg cccggcgctg cgggagatct tcgagcggca
      781 gtcgcattcg gacgtccggc gcctgctgct gtacgtggtg ccgacggcgg aacgcgaggc
      841 cgagcgggtc acggtcgatc cggagcggcc accgctgctg ggcagcgcgc tcagcaaggt
      901 cgtcggcacc gtgctgagcc agacgatcag cgccgagctg gaggatctga ccaggcacaa
      961 cgacgccgtc gtccggacca gggggactcg cgtttccctt gccgcgatgg gggttcgcgg
     1021 cggcccggac tctctggtcg accagcggct gatgaacgac taccgcgacc ggcgggtgca
     1081 ggaggacgcc accgcgctcg tccgtgaggc gacccgcagg ctctccctgt ccgatgtgga
     1141 ggatcagggc cgtcagtggg cgtccggcac ggcggcgcag ctgcgcgcgg ccgcggcggc
     1201 cgggctgcgg gacggcctgc cggtcgagcc gccgaaggac acctgcgaac tcgcggatct
     1261 ggtcgccttc cgcaccaccg cgctcgacga ttcggtcgcc accgggctgc aactggtgaa
     1321 cgcgggcttc cggctcgacc cgtcgccgga gcaggccgtg cagctcaacc gttgccgggt
     1381 gctgctgcac gaagcgcggc acaaggccgc gcgggggcag cggctcgcgg gctggatcac
     1441 cgaacaggag ccgcctgcct ccgacgtcac gctggcggcc tggatcgaag gcctcgccaa
     1501 ggactgggcc gcgctcggcc gctcggaaac cctgaaggag gcgtggccgc tggtcgtcgc
     1561 cgccctccgg caggccacgc cgatcctttt gccgctggcg cgggcgaaac ccggcaccga
     1621 ggccgccgac acggtcagca cgctcctggc ctggacgggc ctcactgcgg acgacaagag
     1681 cgccggtgat cccgtcgtca cctcgcggct cgtgcgcctg cacatcgcga cccgggggct
     1741 gctcgcgcag gcgccgtcgg tcgatcagcg ggtcgacctc gtccaggtga gcgcggattc
     1801 gcggacgctg atggacatga aacggcgtcg ttcctgggac aagctcaccg ggatgcaggc
     1861 cgactacttc ggcgcgttct acaaggcgtc gtggcgcgcg aacgactgga tgtggggccg
     1921 ggtcgacggc gcgggctggc tggtgcagtg cctgctggac ccgaaacgcc tgcgcctgct
     1981 gcgcgacgtc gttggacggg aggcgttccg gacgcgggtg cgggacacct tcaaggcgat
     2041 cggctggcgg cagccgggta cggaagacgg gctgtcgcaa gaggaagcgg aggccctgcg
     2101 cgcgcaattg gccggtgagc tggctttcct cggtctcgac ggcgaactcg ccgacgtgga
     2161 aaccgaagcc gccctgccga tcagcatgcc ggtgacggcg atggtgctcg ccagggtgcg
     2221 gcaactggag atcgcccgcg aagagctgcc gtgtgtcggc ctccactgcg ggcacgacgc
     2281 ggaaacggcc aagggcaacg ggaaaccgtc ggaacggttc aggaaactcg tcgagaacga
     2341 gcccgaaacc gacgagcaga cccagcgcgc gttccaggcg tgccaggtct ccggcgaacg
     2401 gttcgaacac gaacgcggga cgatgcttct caccaagacg ctggtcaaag ccggcgccgc
     2461 cgggctcaac gcggccgccg gggcgacgcg ggtaccgaag tccgtccagc cggccgcgac
     2521 cttcgccaag gccgccgggc gcagcgcgtg gtggatcacc cgcggcgcgg ccaccctgcc
     2581 gtcgccgtgg aacgtgctcg ccgcactcat caccgtgctg gccggtttcg tcctcggtgg
     2641 acagggcggc cccgtcctgc agtgggtcgg cgtgccggtg gccgcgggcg ccatcgtgtt
     2701 cctggtggtg agcctgatga cgctgcgcaa gacgtggcgg atggtgctga cggtgctcgg
     2761 tgtgctcgtg gtggcggcct tgctgttcgc cgcgttcctg ccaccggtgc gcgacccgct
     2821 gttcggctgg ctcggcgacg tcgtcgcggg ctggcggcgc ggcgaggcgc cggtctggtg
     2881 gctggtggtc tgcctgttgc tcgtgctacc cgccatctgg acgccgctcg gctcgctcgg
     2941 gggtcgcaag cgacggcgtg aatagcggaa ccgggcgtcg aatagcgccc tgaccctgcg
     3001 acgaagcggt aacggttggt atcgtcacat gcagtgagac cgcgacgaag cggccttacc
     3061 cacaagtgct gcgcttcaaa cgttaccgtt acctttgtca cgtgtgtttc ggggttgttg
     3121 caggtagccg cgttgccgcc gccgcgctga tctgaccgcg cccgaatcct ccggtacgtt
     3181 tcttggacat gtccggaaag cacttgatcg aaaatccgac caaggcagac ggtgccgcgc
     3241 tctggagaat cgcccgcgat tcgaagaagc tggatctcaa tacgccttac gcctatttgc
     3301 tgtggtgcca cgatttcgcc gacacctcgg tcgtcgcgaa agtcgacggg accccggtcg
     3361 gcttcgtgat cggataccgc aagcaggaca ccggcttcgt ctggcaggtc gccgtcgacg
     3421 cgtcccagcg aggaaaaggc ctggccggag ctctgctcga cgagctgttc ggccggttga
     3481 ccgcccaggg cgtccggtac ctcgaaacga cgatcacccc ggacaacgag gcttcgatcc
     3541 ggttgttcgc ctcgttcgcc aagcggtggg acgccaccgt ggaacgccag gatctgttct
     3601 ccgcgacgga tttcccggcc gaggaaggaa cgcatgagca ggaggacctg tatcgaatcg
     3661 gtccgctcag cagtcaatag aacgtaaatc cggggatccc agggagaaag aaaacgtgat
     3721 gagcattttc gaggaactcg aatccgaggt acgcagttac agccgcggct ggcccgtcgt
     3781 gttcgaccgg gcgcagggga gctacctgta cgacgaggac ggcaaggcgt atctcgactt
     3841 cttcgccggg gccggcgcgc tcaactacgg gcacaacaac cccgcgctga agcgggcgct
     3901 gatcgactac atcgcccgtg acggcgtgac gcacgcgctc gacatgttca cggtggccaa
     3961 gcgggacttc ctccagacgt tccaggagaa gatcctcaag ccgcgcgagc tcgactacaa
     4021 ggtcgtcttc cccggccccg gcggcgcgaa cgcggtcgag gccgcgctga agctcgcccg
     4081 caaggtgacc ggcaaggaat cggtcatcaa cttcaccaac gccttccacg ggatgacgct
     4141 gggcgccctg tcggtgaccg gtaactcgat gaagcgcggc ggcgcgggtg tcccgctggt
     4201 gcacgccacc cccatgccgt acgacaacta cttcgacggc tccatcccgg acttcctcta
     4261 cttcgagaag ctgctcgaag actccggcag cggcctgaac gagcccgccg ccgtgatcgt
     4321 cgagggcgtg cagggcgagg gcggtatcaa cgccgcgcgc gtcgagtggc tcaaggccct
     4381 ggacgacctg tgcaagaagc acggcatcct gctgatcctc gacgacgtcc agatgggctg
     4441 cggccgcacc ggcccgttct tcagcttcga ggacgcgggc atcaagcccg acatcgtctg
     4501 cctgtcgaag tccatcggcg gctacggcat cccgatggcg ctgacgctga tccgcccgga
     4561 cctcgacgtc tgggagccgg gtgagcacaa cggcaccttc cgcggcatca gcccggcgtt
     4621 cgtcaccgcg accgaggcgc tgcgcgtcta ctggagcgac gacgagctgg agaagtcgac
     4681 caaggccaag ggcgagcgca tcggcgccgc cttccagggc atcgtcgagg cgtacccgga
     4741 cgcgaacctg ttcgcgaagg gccgcggcct cgcccggggc atcgagttcg ccaatggcga
     4801 cctcgcgggc aaggtctgcg ccgccgcgtt cgaacgcggg ctgctgatgg agacctccgg
     4861 ccccgacggc gaggtcatga aggtcctccc gccgctcacg ctgacggacg acgagctcac
     4921 gaagggcctg tcgatcatcg acgaggccgt cgccactgtc ctgaactgac ccaccaccag
     4981 gaacgagagg gagttttccg ttgcttgtca gaacactcga cgaggtcacc gacaccgacg
     5041 ccgacatcaa gaccccgaac tggcgcagca agcgcatcgt gctggccaag gagggcgtcg
     5101 gtttctcggt gcacgagacc acgctgtacg ccgggacggt caacgacttc tggtacgcga
     5161 accacatcga agcggtgttc atcacctctg gtgagggcga gctcgaaaac accgcgaccg
     5221 gcgaggtctt ccagctgaag ccgggcacgt tgtacctgct caacgaccac gacaagcacc
     5281 aggtccggcc caagaccgag atcaagtgcg tctgcgtgtt caacccgccc atcaccggcc
     5341 gcgaggtcca cgacgaaaac ggcgtgtacc cgctcgtcac cgaaccctag aaatcgctcg
     5401 aggaggcgac cgcattgacg ctgaccgaca cccgagtcga cgacagttac ccgacccgca
     5461 tcacgggtaa gcccgaacac ctgcccagga cgcaccccac cgtctggggc gccgaggccg
     5521 acggtcccat ggacgcggcc acgctcgcca accacgaaac gcgtggttac accgtcgtcg
     5581 accagttgct gtcgcccggc gaggtccagg cctactggca ggaactcgtc aggatgtcct
     5641 ccggggacca cagcggcgac gagcgcgtca tcaccgaggc caagaccggt gaggtccgct
     5701 ccatcttcga cgtccacgag accagcgagc tgatcgcgga actcgtccgg gacccgcgcg
     5761 tgctcgaccg ggcccggcag atcctcggct ccgaggtcta catccaccag agccgcgtga
     5821 actacatgcc cggtttcaag ggcaccgggt tctactggca ctcggacttc gagacctggc
     5881 acgccgagga cggcatgccc gcgccccgcg cggtcagctg ctccatcgcg ctcaccgaca
     5941 actatccgtt caacggcggg ctgatggtga tgccgggttc gcagcggacc ttcgtgcagt
     6001 gcgccgggga aacccccgag aacaactaca agagttcgct gaaagagcag cgggtcggcg
     6061 tcccgagcga ggaagacatc accgcgctcg ccgccgaata cggaatcgac caattcaccg
     6121 ggcaggccgg ttccgcgctc tggttcgatt cgaatgtgat gcacggttcg tcgaacaaca
     6181 tcacgccgta cccgaggtcg aacatcttcg tcgtgttcaa cagcgtcgaa aacgcgctgc
     6241 gggagccgtt cgccgcgatc gaaccgcgtc cggcgttcat cgccggccgc gactcgaccc
     6301 cgctgtcgcg ctgacgaaac cgcaggtcgc gaaggccatg gcggggcgct tgtagccgtc
     6361 ggtgtgcgcc ctgttacgtt gtcgccacgc gtgcgggcca gtgggctcgc tcacgtaggt
     6421 gttgtcgacg accgaacggg ttcttcgctc ggggtgagcc cacccggacg gacgtggacg
     6481 ccgatgtccg gtgtgggtcg cgcccccagg cggcccaaat cggacatctc ccggctccgt
     6541 gccatcagat cgggactgat ggcacggagc ctcttctttt tcctcgtgtc caggctcggt
     6601 ccgtgaaggc ctccttgagg gactctgggt ccctcaagga ggccttcacg gacttcaggt
     6661 catgaggagt gctccggaac gtaagtccat agtggacaga gagtcccgtg gggcatttgt
     6721 ccgtatcgcg aggtcgtgaa ggcccccttc cttaccctga aagtagggaa gggggccttc
     6781 acgacctcgc gcgtcctggt tggcggcctc ttcgcgcggt aggttggcta ctccggtaag
     6841 gggagagatc ggaggagggg gcttgtcatg ggcgaaagcg gttcccctgc ccgcttccaa
     6901 ctcctcggtc ccgtccggct cctcgacggc gaacggccgg tccccgtcgg cggtcccggc
     6961 gtccgcgggc tcctcgcgtt gctggcgctg aaggtcaaca aggtcgtcgc gctcgacgag
     7021 atcatcgacg cgctctgggg gcacgacccg ccggccacgg cccgcacgat cgtccacggc
     7081 aacgtctcgc atctgcggcg cgtgctccgg ggcatccagg gcgatcatcc gcacggcgcc
     7141 cggatcctca ccgcgccgcc cggctaccag ctcaccgtcg aacccgagcg gatcgacgtc
     7201 caccgcgccc gggccctgct ggagcgggct tccgcggaag agccggagaa ggcggccgaa
     7261 ctgctcaccg aggccctcgc gctctggcag gggcccgcgc tcgccggtgt gccggggtcg
     7321 gtccgcgctc ccgaactgga ggacctgcgg ctcgccgtcc acggcgccag ggtcgacgcg
     7381 gacctcgaac tgggcaggca cgcggaactg atcgtcgagc tcagtccgct cgtccgggcg
     7441 aacccgctcg ccgagcgcac ggccggtcag ctgatgcgcg cgctctacca cgccggccgc
     7501 cgcggcgacg cgctcgagct ctatcgcacg gtgtccaggg cgaccctgcg cacgctcggc
     7561 gtcgaacccg gcgcggatct gcgctggctg cacgaacgcg tcctcaacga cgatctgccg
     7621 gtcaaggtcg cggcggaacc gaaggccgag gcggcgcccg tccagcagtt gccgcccgcg
     7681 gtgccgaacc tcgccggacg cgaggccgag ctggcctggc tcgacgaact cgccgagcgc
     7741 gccgaagcgg gtgagaccgc ggtcggcgtg gtcaccggga cggccgggat cggcaagagc
     7801 agcctggtgg tgtggtgggc gcatcgtgcg ggccgccgct tccccgacgg catccttttc
     7861 gcctcgctgc ggggtttcga cccgcaccat ccgccgctgg agcccgccga cctgctcacc
     7921 cagttcctgc tcgggctcgg cgtcccggcc gaaggcgtcc cggaacaggt ccacgaacgc
     7981 gtcgccctct accgctcgat gatcgccgga cgccggatgc tggtcatcct cgacaacgcc
     8041 cggagcgccg agcaggtccg gccgttgctg ccgcccggtt cgcggtcgat gacgctggtg
     8101 accagccgct cccggctcga cgggctcgcc gtgtccaacg ccgccaaact gcgggtgctc
     8161 ggcaccctcg ctcccggcga cgccgtccgg ctgatcgagg aactcgccgg gcccgccggg
     8221 ttcgacctca accacgcgct cgcccggctc tgcggctatc tcccgctggc gctgcggatc
     8281 gccggtgccc ggctcgccgc cagcgcgcag tggtccgcgc aggatctggt cgacgaactc
     8341 ggcaacgaac ggaccaggct ggccgcactc gacgtcgaag gcccggacga cggcgtccgc
     8401 gcggcgttcg acgtctcgtt ccgggggctg ccgccggagg tcgcgaacac gttcctgcgg
     8461 ctcggcgtgg tccagtgcgt ctcggccggt tcgcatctga cggcggcgat cggcgggatc
     8521 accgtcgccg aggcgcggcg gcatctgcgg gtgctcaccg cgcacaacct gctcgccgaa
     8581 accggccgcg actccttcgt cccgcacgac ctcgtccgcc tgttcctccg cgaactggcg
     8641 gagaacgaac tcgacgaggc cgaccgggac gacgtcctgt cgcgctcggt gcggttctat
     8701 caggccgtcg ccgaccgggc ccggcgcaag atgctgcgga tcgtcgaccc gctcgacttc
     8761 accggggcgc tgaccgacgc gcagacgccg ccgatcggtt ccttcgacga ggcgcaggac
     8821 tggttcaccg cggagtggga gaacctgatc gaggtcctcg aagccgcccg cgccgccgga
     8881 cggcacgacg acgtctggcg gctggccagg gtcgcgcaca cctatcgcgc ggtctatccg
     8941 ctgctggacg aatggacgcg gctggtcgag atcggcctgg aggccgccga gcggtccggc
     9001 gacgtcctcg gccgctgctg gatgctgatc tcccggtgcg ccatcgcgct caccttcgag
     9061 ctgccgcagg gctgcctcgc cgacgccgaa cgcgcgctgg aactcgcggc cgcgatcggc
     9121 gacgaccggc tgatcgtctc ggcgaacatc catctcggct ccgcgctgac cctgctggga
     9181 cggcacgacg aggcgatcag gacgctgcgg cgcgcggtgg aggagaccga gcggaccggc
     9241 gatctcgaat tgcgcgggca ggcgctcaac aactgcgccg aggccgaaaa gcgggcaggc
     9301 cggttcatcg aggcgatcgg gcatcaggtc gcctccctgg agatcgaccg gacgctcggc
     9361 gacgaaagct acgtcgtcgt ctcgctgaac aatctcgccg aattgagcat gcggatcggc
     9421 gaactcgcgg cggccgagcg gtacgtgtgg gaagccgtgg acctgacgat cagcaggcgg
     9481 ttcgtcctcc aggagggtgt cctgcggctg acgctgggcc gggtgctgcg ggccgggtcg
     9541 gatgtggacg gtgcccgcga gcaattcgcg ctcgcgctga agatcctctc ggacgtcaac
     9601 cccaagctcg ccaccctggt ccgtgcggaa ctcaccgacc tcgacgaaag tccgtgagtg
     9661 atccttagcg gattcttagt ggcctcgccg cgcacgcgcg tagggtccga ttcagtgtcg
     9721 atcgagccct aggggagggg cacgccgtag tgatcggcgc tcgggcgggc tcgttccggg
     9781 tccgtctgaa gagccgccag gagcggctcg tggcggtccg gccggtggag gtgggggcga
     9841 taccggccgg ccgccaggcg gcgcgaaacg cggagggtca gccggtgccg gcggtgcaca
     9901 gggcgatggt ctggcccttg ttctccgcca ccttcttgcc gttgaccagg atcgtgcagg
     9961 cgatctggtt gctgaccgtc gagttccggg tgtccgcggt gacggtgagc aggtagtcgc
    10021 ccgagttgaa ggacgccgtg ccgcgccatt cgtcggtgct ggccggggtc gtctcggtcc
    10081 gctggtcgtt gagcgagccg taccggatgg tcgcgccgcc ggaggcgctg acgacgaact
    10141 cgacggtgtt cttgtccccg gcgaccgccg ggacgtgcag tcccgactca ccggtggcga
    10201 agaaactggt gtagaccagt aacccgcccg cggccagcag tccggcgatc gaaagcgcga
    10261 gccccgcgat ccccattccc ttcttgtccg cttcaccctt cttgattttg gtcaaaccga
    10321 tcacggaaag aatcaaaccg gtcacggcga gcggccatgc ggcgacgccg gcgaccggga
    10381 cgaaggcgac
//
