LOCUS       NZ_CP024972            10816 bp    DNA     circular CON 18-AUG-2024
DEFINITION  Amycolatopsis sp. TNS106 chromosome, complete genome.
ACCESSION   NZ_CP024972
VERSION     NZ_CP024972.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. TNS106
  ORGANISM  Amycolatopsis sp. TNS106
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 8806031)
  AUTHORS   Liu,K. and Tao,M.
  TITLE     Discovery of new natural product by Amycolatopsis speibonae genome
            mining
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8806031)
  AUTHORS   Liu,K. and Tao,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, Shanghai
            200030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP Assembly v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 116.0x
            Sequencing Technology             :: Illumina HiSeq 4000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019330265.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 03:49:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,321
            CDSs (total)                      :: 8,231
            Genes (coding)                    :: 8,179
            CDSs (with protein)               :: 8,179
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 20 of 52
            Pseudo Genes (incomplete)         :: 35 of 52
            Pseudo Genes (internal stop)      :: 4 of 52
            Pseudo Genes (multiple problems)  :: 6 of 52
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:02:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3599644
            Orig. end                         :: 3610460
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024972.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10816
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="21"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10816
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10816
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5816](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5816
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(180..196)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.52"
                     /tool="antismash"
     misc_feature    complement(379..394)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.07"
                     /tool="antismash"
     misc_feature    complement(794..803)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(1255..1270)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.18"
                     /tool="antismash"
     misc_feature    1256..1269
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.03"
                     /tool="antismash"
     misc_feature    complement(1357..1371)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.42"
                     /tool="antismash"
     misc_feature    complement(1455..1468)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.74"
                     /tool="antismash"
     gene            1531..1836
                     /locus_tag="CVV72_RS15980"
                     /old_locus_tag="CVV72_15905"
     CDS             1531..1836
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033523.1"
                     /locus_tag="CVV72_RS15980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_15905"
                     /product="hypothetical protein"
                     /protein_id="WP_034318111.1"
                     /transl_table=11
                     /translation="MADSGPFGPGQNPRFQEDLVGLDPYDPEARAFAEHLDRIERCGPN
                     FTVEACLDGVSDFADSSNRIGGLRWWISALVVILIVLGVMVTAWDIIGRAIAWLAG"
     gene            1856..2278
                     /gene="msrB"
                     /locus_tag="CVV72_RS15985"
                     /old_locus_tag="CVV72_15910"
     CDS             1856..2278
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333001.1"
                     /locus_tag="CVV72_RS15985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_15910"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_034318341.1"
                     /transl_table=11
                     /translation="MKPVVGATPRVVKSEQEWREQLSPDEYAVLRQAGTERPFTGEYTD
                     EKTTGVYQCRACGAELFRSDTKFESHCGWPSFYDPADTDAVLLREDTTMGMRRIEVLCK
                     SCHSHLGHVFEGEGYATPTDQRYCINSISLKLVPES"
     gene            2348..3190
                     /locus_tag="CVV72_RS15990"
                     /old_locus_tag="CVV72_15915"
     CDS             2348..3190
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="CVV72_RS15990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_15915"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_219153157.1"
                     /transl_table=11
                     /translation="MNEPWGISGPAFAWLYGCLVVLPFLFAGLWAQSLRRRAADEWTPP
                     GPYHLAALDGGTERVADVVVATMVAGEQIRLDSRGRVTVLRAASGDPLTRAAAELVPRA
                     GAGGLYRLKKLLSRSAAVQEVRNELQLSGLQVDERRRRTGWLVALIVYLPVLALGLARF
                     ANALPLGRPAGVLAGLLLAVVAAIAGTVLLLRPSRAVLPTAAGLRVLGAARRNPALLPG
                     VAGAVALGGLAAYPDPVMADMLVRSGDATAKGSAGSSSGGSGCASGSSCGGGGGGCGG"
     gene            3183..3962
                     /locus_tag="CVV72_RS15995"
                     /old_locus_tag="CVV72_15920"
     CDS             3183..3962
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="CVV72_RS15995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_15920"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_219153158.1"
                     /transl_table=11
                     /translation="MADFGWLHAPAIALPVTVAALHVLRLRRGFHDGDGLPSVRHVAAL
                     AGGASRVTETVVASMLERDQARLGGSGRIYRTPLVPVDPLGLELAGHLSAFPDHELRKR
                     MSHGPAMRALWAELGRRGLVVAEGRRRLTWRIAAAVYAVLLGVGTVRWIATDGTVVLTV
                     LLVVALALLIVSLRLGRTDHEGRTTAAGRAVLAEAESDRSLVSGAAGAVAIGGFAAYPD
                     RQVAAILSRYGGRPAKDTRTFGWGDGPGSSTCGTGGH"
     gene            4076..5011
                     /locus_tag="CVV72_RS16000"
                     /old_locus_tag="CVV72_15925"
     CDS             4076..5011
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033527.1"
                     /locus_tag="CVV72_RS16000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_15925"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_219153159.1"
                     /transl_table=11
                     /translation="MEDTWGIPGPAFLVIYGALLVFPLVVGLVWTVAAKLGAKVPRATV
                     SGPEPTVYELAYLAGGPDRVVDTAIAALVDRGTLRVSSSKQLQLTGSLPADPIEKAVAK
                     GARPGYNATTRGIRDRLRMSGPMQALAKGLEERGLVVADRSPRIRQVVFFLYLAVLVLG
                     VVRLVAGISGDRPVGFLIPLLIAAVFATLIARAVKNKRSGPRPTSEGGRILHRARSVHG
                     RERKRGIPNGPGYAGAAGGALLGGAAAAVAFGGLAYYPDEELSAALIPPPVPGGFGGGS
                     SGGGSTCSTGGGSSCSSGGGSSCGGGGCGG"
     gene            5001..5816
                     /locus_tag="CVV72_RS16005"
                     /old_locus_tag="CVV72_15930"
     CDS             5001..5816
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033528.1"
                     /locus_tag="CVV72_RS16005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_15930"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_219153160.1"
                     /sec_met_domain="DUF692 (E-value: 1.3e-81, bitscore: 272.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVADAPGRLGIGIGWRHELDLSIARLPGVDWVEVVAENLHAGHLP
                     ETLVALRDKGMPVLPHAVSLSLGGAEPLDTARVEHLAEVTRALGAPMASDHVCFVRAGG
                     LDSGHLMPLPRTREALDVLVDNVKLTQSIVEVPFALENIAAVLDWPDAEFTESEFLREL
                     TDRTGCLLIIDVANLYANARNLGTDPERFLDEIPWERLAYTHMAGGVERDGVYHDTHAH
                     PVVPAVLDLLRELRRRTEPPGVLLERDDDYPSDTELAAELAALREAVSP"
     gene            5813..6157
                     /locus_tag="CVV72_RS16010"
                     /old_locus_tag="CVV72_15935"
     CDS             5813..6157
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033529.1"
                     /locus_tag="CVV72_RS16010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_15935"
                     /product="hypothetical protein"
                     /protein_id="WP_219153161.1"
                     /transl_table=11
                     /translation="MTSPRERLAAQQAELLKALLAGGDAPAGFDADRLRIEANVLRNKQ
                     SRLAAYLRPDLAEALGDRFAALFREYATSHPKTDAIRARAYADAFGAWLVDRGEVPKPR
                     GRFSTWLRRN"
     gene            complement(6177..7523)
                     /locus_tag="CVV72_RS16015"
                     /old_locus_tag="CVV72_15940"
     CDS             complement(6177..7523)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1189: L-carnitine dehydratase/bile acid-inducible"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033530.1"
                     /locus_tag="CVV72_RS16015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_15940"
                     /product="CoA transferase"
                     /protein_id="WP_219153162.1"
                     /transl_table=11
                     /translation="MDTRVWTTLTGETLAEDAVEITGPDSVLPGTFRVEEAATTSIAAA
                     TLAAGELLRLRGIEPGKVSVDTRHAAASFHSEQYIRVDGEPVPFSAPLSGDYRANDGWV
                     RLHCNYPRHVSAVCWGLGVPATHAAFEKVVAAKSKYEVESAVIGAGGAAAVMRTREEWL
                     AHKQGQASSALPVAEIRPLGPSEPVRLFESDRPLGGVRILDLTHVIAGPVAGRVLAAHG
                     ATVMHIGSAQLPTVPPLAVDTGMGKLSAYVDLETEAGRSRLRKLIARADVVLQGFRPGS
                     LVAKGFSPEAIAELRPGIVVADLSAYGWEGPWARRRGFDSLVQMASGIADEGGKAAGLD
                     GPGPLPVQALDHATGWLTAAAIMTAVRRAATEGGSQHVRTSLAGTGEWLNSLGRKEAGP
                     AGFDGGEWLEDVEGRLGRITRVKMAGTLPSSNPQWTEATHVPGSDRPVW"
     gene            complement(7535..8227)
                     /gene="hemQ"
                     /locus_tag="CVV72_RS16020"
                     /old_locus_tag="CVV72_15945"
     CDS             complement(7535..8227)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="CVV72_RS16020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_15945"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_126731581.1"
                     /transl_table=11
                     /translation="MARLNYNELNDTIRYTAWSVFRAEQGKLGEDRAKATAETTEYLDA
                     LEAKGVVVRGVYDLSGLRADADYMIWWHAEEIEQVQAAYSGFRHTPLGRASAPVWSQVA
                     LHRPAEFNKSHIPAFLAGEEARKFICVYPFVRSYEWYLLPDDERRKMLADHGKEARDYP
                     DVRANTVASFALGDYEWILAFEADELHRIVDLMRHLRGTEARLHVREEIPFYTGTKVEP
                     AELIALLP"
     gene            complement(8253..9632)
                     /gene="hemG"
                     /locus_tag="CVV72_RS16025"
                     /old_locus_tag="CVV72_15950"
     CDS             complement(8253..9632)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332996.1"
                     /locus_tag="CVV72_RS16025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_15950"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_219153163.1"
                     /transl_table=11
                     /translation="MKTVAVVGGGISGLTAAYRLRTLLGEAARIVVFEATDSLGGKLRT
                     VEFAGERYDVGAEAFLARRPEALALVREVGLDESLVHPTKARAKIHAGGSVLGLPPGTV
                     MGVPASADAVAGVLSEKGRALVEAEASLPPLKLHAEDVPLGELLRERFGDELVDRLVDP
                     LLGGVYAGGADGLGLRATMPGLASAIDRGARSLTEGAASLMPASPSTAPVFGTLLGGLG
                     TLIARLAELSRAEIRTGTTVTALERTPTGWKVGDLDADSVLLAVPAPSARRLLDGVAEV
                     ASTVFAKIELASMAVVALALPPGTALPESSGVLIGAGERDAAGKPFAAKAFTFSSRKWA
                     QYGEGPVLVRGSVGRFGEPGALHADDDELVRVVRDDLARLTGVTAEPIDTLVTRWGGGL
                     PQYGIGHLVRVERIERAVAAVPGLAVAGATLHGVGIPACIATADAAARRIAGITPTHEG
                     S"
     gene            complement(9629..10702)
                     /gene="hemE"
                     /locus_tag="CVV72_RS16030"
                     /old_locus_tag="CVV72_15955"
     CDS             complement(9629..10702)
                     /EC_number="4.1.1.37"
                     /GO_function="GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005156993.1"
                     /locus_tag="CVV72_RS16030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_15955"
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="WP_219153164.1"
                     /transl_table=11
                     /translation="MSPSVASPAQTVPAARRALPGAPFLAAARGERPAHTPVWFMRQAG
                     RSLPEYRALREGTSMFEACFDPEMLAEITLQPIRRHGVDAAILFSDIVVPLKAAGLDID
                     IVAGTGPVVAEPIRDAAGVRALPVLEPEQVERVAEGVRLLVGSLGETPLIGFAGAPFTL
                     ASYLIEGGPSRNHEHTKALMHSEPELWHELAGRLADMAITSLSAQLDAGADAIQLFDSW
                     AGALSERDYREFVLPHSAKVLSAVAGYGVPRIHFGVGTGELLVAMRDAGADVVGVDWRI
                     PLDEAVRRLGGGVVQGNLDPALLHAAWPVLEAEVRRIHAEGRAADGHIFNLGHGVLPGV
                     DPDVLTRVVGLVHELQP"
ORIGIN
        1 agcatccgaa cgcgatcgtg gtgttcgaca tcgccgagca cggcggcgat ccctgcctgg
       61 tgatggagta cctgaacggc ccgagtctct cggcgatcat ctccgaagaa ggcacgctgc
      121 cgctcgggcg cgtcgcgaag atcggcgagc aggtcgcgtc ggcgctggtg gccgcgcacc
      181 gcgccgggat cgtgcaccgc gacgtcaaac cgggcaacat cctgatcgac gaggccggga
      241 ccgcgaagat caccgacttc ggcatctccc gggccgccgg cgacatgacg ctcacccaga
      301 ccggcctgat cggcggcacc ccggcgtatc tcgcgcccga gctggcgcgc ggctccgacc
      361 cggtgcccag ctctgacgtc ttcgctctcg gcgcgacgct ctaccaggcg atcgagggcc
      421 agaccccgta cgggaacagc tcgaaccagc tggcgctgct gtacgcggcc gcgaatggcc
      481 agatcaaccc gccgcagcag gcgggccccg cgacggcgct gctgatgagc ctcctgcgca
      541 ccgagcccac cgagcgtccg accatggccg aggccaggga acgtctcgcc gcgctggcgg
      601 cgaacgaatc cggcgcgggc atggccgcgt cgccgcggct tttcggagcg ggcggcggta
      661 gacagcccgc cgtgcccgac accccggggc acggcatccc cggcggagag cggccgccgt
      721 ggcagcggac cgacaagccc gccgagtccg ccgcgggcgc tcccccggct ccggccaaga
      781 cccacacgcc gacggccgcc ttcatcccga tgcgctcgcc gtccgccccg ccgaacacgc
      841 cgccgcgtcc ggtcgccgcg atgagcccgc cgccgtccgc ggccgccccg atgcggccgt
      901 ccccggccaa gcccgccgac gccaaacgca aggccgtcct gttcgcgggc gccggtgccg
      961 ccgtcgtggt ggtcgccgtg atcgtgttcc tggtcctcaa ttcgggcaac gggaccgaca
     1021 accagggtgg caacaacgcc ggggcgaacc cgaagaccag tgtctcttcg cccaagtcga
     1081 gtgcgccgtc ctcgtcgcgc ccctcgccgg cgggcctggg gaagaccccc agcgagaagg
     1141 tcacgagcta ctcgagcgtg ggctccttcc tcctggactt cttcgatacc ggcaagatcg
     1201 gcacgtccga gagctggaac aagctcacgg acgccgcgaa ggccgtctac ggctcagaac
     1261 aggctttcgc cgactactgg gccgcgcaca aggtcaccgg ctcggagaag gcacgagccg
     1321 acagcggcgg cggtaacccc gacgggtcga tcgacatgaa catcacgatc aacggcggcc
     1381 cgcggccggc gctgcgtgtg gtccaggtcg gcggccagta cctcatcgac gccgacacgc
     1441 gagtactcgg caactgacgc agcgtggcgg tggtgagacc cgcctcgctg gtggtaggcc
     1501 aatcgccgct cgtggcttac tgttgccggt atggccgata gcggaccttt cggacctggc
     1561 cagaatcctc gcttccagga ggatctggta ggcctcgacc cgtacgatcc cgaagcccgt
     1621 gccttcgccg agcatctgga ccgcatcgaa cgctgcggcc cgaatttcac cgtggaagcc
     1681 tgcctcgacg gtgtctccga tttcgccgac tccagcaacc ggatcggcgg tctgcgctgg
     1741 tggatttcgg cactcgtcgt gatcctcatc gtgctcggcg tgatggtcac cgcatgggac
     1801 atcatcggcc gggccatcgc gtggctcgcg gggtaacgtg gaaccggtga aaggcatgaa
     1861 acccgttgtc ggagccacgc cccgcgtggt caagtccgag caggaatggc gggaacagct
     1921 cagccccgac gagtacgccg tgctccgcca ggcgggcacc gagcgcccct tcaccggtga
     1981 gtacaccgac gagaagacca ccggtgtcta ccagtgccga gcctgtggcg ccgagctgtt
     2041 ccgcagcgac acgaaattcg aaagccactg tggctggccg tcgttctacg acccggccga
     2101 caccgacgcc gtcctcctcc gtgaggacac caccatgggg atgcgccgga tcgaggtgct
     2161 gtgcaagtcg tgccacagcc acctcgggca cgtcttcgaa ggcgagggct acgccacgcc
     2221 gacggatcag cggtactgca tcaactcgat ctcgctgaag ctcgttcccg agtcgtaacc
     2281 aaccgaagcg aaccaccgcc ctccccgacg cgttcatggt gaacaacgtc agggagggcg
     2341 gtgtcgtatg aacgaaccat gggggatctc gggaccggct ttcgcgtggc tgtacggctg
     2401 cctcgtcgtg ctgccgttcc tcttcgccgg actgtgggcc cagtcgctgc ggcgtcgtgc
     2461 cgccgacgag tggacgccgc cgggccccta ccacctcgcc gcgctggacg gtggcaccga
     2521 acgggtcgcc gatgtcgtgg tggccaccat ggtcgccggt gagcagatac ggctggacag
     2581 ccgagggcgc gtcacggtcc tccgcgccgc atcgggggat ccgctcacgc gcgccgccgc
     2641 cgaactcgtt ccgcgagcgg gtgccggcgg gctctaccgg ttgaagaagc tgctgagccg
     2701 gagcgccgcc gtccaggaag tgcgcaacga acttcagctg agcggcctgc aagtggacga
     2761 acgccgccgt cgcaccgggt ggctggtggc cttgatcgtc tacctccccg tgctcgcgct
     2821 cgggctggcg aggttcgcca acgccctccc gctgggtcgc ccggccgggg tgctggcggg
     2881 tctcctcctc gcggtggtcg cggccatcgc cgggaccgtc cttctcctgc ggccgagccg
     2941 cgcagtcctg ccaaccgcgg ccggtctgcg cgtcctcggc gcggcccggc ggaatcctgc
     3001 tctgctcccc ggagtggcgg gcgcggtggc gctcggcggc ttggcggcgt atcccgatcc
     3061 ggtcatggcg gacatgctgg tcaggagcgg cgacgccacc gccaaggggt cggcgggttc
     3121 gtcgagcggg ggaagcggct gcgcgagcgg ttcttcctgc ggaggaggcg gaggtgggtg
     3181 cggtggctga cttcgggtgg ctccacgcac cggcgatcgc gcttcccgtc acggtggcgg
     3241 cgcttcatgt gcttcggctc cggcgcggct ttcacgacgg cgacgggttg ccgagcgtgc
     3301 ggcatgtggc ggccttggcg ggtggcgcct cgcgggtcac cgaaacggtg gtcgcctcga
     3361 tgctcgaacg ggaccaggcc cggctcggtg gttcgggcag gatctaccgc actcccctgg
     3421 ttccggtcga cccactgggg ctggagctcg ccggtcacct gtcggccttc cccgaccacg
     3481 agttgcggaa gaggatgagc cacggcccgg cgatgcgagc gctgtgggcc gagttgggcc
     3541 gccggggcct ggtcgtcgcc gaaggacgcc gacggctcac gtggaggatc gccgccgccg
     3601 tctacgccgt ccttctgggg gtcgggacgg tccggtggat cgccaccgac ggcaccgtgg
     3661 tcctgaccgt gctcctcgtc gtggcactgg ccttgctgat cgtctccctc cggctcggcc
     3721 ggaccgatca cgagggccgg acgacggccg cgggccgcgc ggtgctggcc gaggcggagt
     3781 cggatcgaag cctcgtttcg ggtgcggcgg gcgcggtggc catcggcggc ttcgccgcgt
     3841 atcccgatcg ccaggtcgcc gccatcctga gccgctatgg cgggcggccc gccaaggaca
     3901 cccgcacctt cggctggggt gacggtcccg gttcgagcac ctgcgggacc ggcggccact
     3961 gacggcccaa ctgccgtcgt ggagacccgc tccggatggt cgaatcacgg aaccacgacc
     4021 gggcggaaaa gcgtccgact acggtaagtg cacctgctcg tgaaaggccc gtgcgatgga
     4081 agacacctgg ggcatccccg gccctgcgtt cctggtgatc tatggcgccc tgctggtgtt
     4141 cccgctggtc gtcggtctcg tgtggacggt cgcggccaag ctcggcgcca aggtcccgcg
     4201 cgccaccgtg agcggaccgg aaccgacggt gtacgaactc gcgtatctcg ccggcggccc
     4261 ggaccgcgtg gtcgacacgg ccatcgcggc gctggtcgac cgcgggacgt tgcgggtcag
     4321 cagctcgaag cagctccagc tcaccggctc cctgcccgcc gacccgatcg agaaggccgt
     4381 cgccaagggc gcgcggccgg gctacaacgc cacgacccgc ggcatccgcg accggctgcg
     4441 gatgtcgggg ccgatgcagg cgctcgccaa aggactggag gagcggggac tcgtcgtcgc
     4501 ggaccggtcg ccccggatcc gccaggtcgt gttcttcctg tacctcgccg tgctcgtact
     4561 cggcgtcgtc cgcctggtcg ccgggatctc cggtgaccgc cccgtcggtt tcctcatccc
     4621 gctgctgatc gcggccgttt tcgcgacgct gatcgcccgg gcggtcaaga acaagcggtc
     4681 gggcccgcgg ccgaccagcg aaggcggccg gatcctccac cgggccaggt ccgtccatgg
     4741 ccgggagcgc aaacgcggca tcccgaacgg accgggttac gccggtgcgg ccggcggcgc
     4801 cttgctgggt ggcgccgcgg cggcggtcgc gttcggcggc ctcgcctact acccggacga
     4861 ggaactgagc gcggccctga tcccgcctcc ggtccccggg ggtttcggcg gcggttcgtc
     4921 cggcggcggc tccacgtgca gcaccggcgg aggaagctcg tgcagcagcg gaggcgggag
     4981 ttcctgcgga ggcggcggct gtggtggctg acgcgccggg caggctcggg atcggcatcg
     5041 gctggcggca cgagctggac ctgtcgatcg cgcggctgcc cggcgtcgac tgggtcgagg
     5101 tcgtcgccga gaacctgcac gccgggcacc ttcccgaaac cctggtcgcg ttgcgcgaca
     5161 agggaatgcc ggtcctgccg cacgcggtct cgctctcgct gggcggggcc gagccgctgg
     5221 acaccgcgcg cgtcgagcat ctcgcggagg tcacccgcgc gctcggcgcg ccgatggcca
     5281 gcgaccacgt gtgcttcgtg cgcgcgggcg ggctcgactc gggtcatctg atgccgctcc
     5341 cccgcacccg ggaagcgctg gatgtcctgg tggacaacgt caaactgacg cagtccatcg
     5401 tggaggtccc gttcgcgctg gagaacatcg cggcggtgct ggactggccg gacgcggagt
     5461 tcaccgagtc ggagttcctg cgggagctca ctgaccggac cggctgcctg ctgatcatcg
     5521 acgtcgccaa cctgtacgcc aacgcccgca acctcggtac cgatcccgaa cggttcctcg
     5581 acgagatccc gtgggaacgg ctggcgtaca cgcatatggc cggcggtgtc gaacgcgacg
     5641 gcgtctacca cgacacgcac gcccatcccg tcgtcccggc ggtactggat ctgctgcgcg
     5701 aactgcgccg ccgcaccgag ccaccgggtg tcctgctgga acgcgacgac gactacccgt
     5761 ccgacacgga actggcggcc gagctggccg cgttgcgaga agcggtctcg ccgtgacgtc
     5821 gccccgggag cggctcgccg cccagcaggc ggagctgctc aaggcgctcc tcgcgggagg
     5881 cgacgccccg gcgggcttcg acgcggacag gctccgtatc gaggcgaacg tcctgcggaa
     5941 caagcaaagc aggctcgccg cctacctccg tccggacctc gccgaggccc tcggcgaccg
     6001 gttcgccgcg ctgttccgcg agtacgcgac cagccatccg aagaccgacg cgatccgcgc
     6061 gcgggcctac gccgacgctt tcggcgcctg gctcgtggac cgcggcgaag taccgaagcc
     6121 ccggggccgg ttctcgacct ggctacgccg caattagtca cctgcttgcg gcacgatcac
     6181 cagaccgggc ggtcgcttcc gggtacgtgc gtcgcttcgg tccactgtgg attcgagctc
     6241 ggcagggttc ccgccatctt gacccgggtg atccggccga gccggccttc gacgtcttcg
     6301 agccattcgc cgccgtcgaa gccggcgggc ccggcttcct tgcgccccaa ggagttcagc
     6361 cactcccccg tcccggcgag cgacgtccgg acgtgctggc tgccgccctc ggtggcggcc
     6421 cggcggacgg cggtcatgat cgccgccgcg gtcagccagc cggtcgcgtg gtcgagcgcc
     6481 tgcaccggaa gcggtcccgg gccgtcgagc ccggcggcct tgccgccctc atcggcgatt
     6541 cccgacgcca tctgcaccag gctgtcgaac ccgcgcctgc gcgcccatgg cccttcccag
     6601 ccgtacgccg acaggtccgc gacgacgatg cccggccgga gttcggcgat cgcttccggc
     6661 gagaaaccct tggccaccaa cgaacccggc cggaaaccct ggagaacgac gtcggcgcgg
     6721 gcgatcagct tacggagccg cgagcgtccg gcttcggtct ccaggtcgac gtacgccgag
     6781 agtttgccca tcccggtgtc gacggccagc ggcggcacgg tcggcagctg ggccgacccg
     6841 atgtgcatga cggtggcgcc gtgcgcggcg agcacgcggc ccgcgaccgg cccggcgatg
     6901 acgtgggtca ggtccaggat ccggaccccg cccagcggcc ggtcggactc gaacagccgc
     6961 acgggttccg acggcccgag cggccggatc tcggcgaccg gcaacgcgct cgacgcctgt
     7021 ccttgcttgt gcgccagcca ttcctcgcgc gtgcgcatga cggcggcggc accaccggcg
     7081 ccgatgaccg ccgactcgac ctcgtacttc gacttggccg cgacgacctt ctcgaacgcc
     7141 gcatgtgtcg cgggcacgcc caggccccag cacacggcgg agacgtgccg cgggtagttg
     7201 cagtgcaacc gcacccagcc gtcgttcgcg cggtagtcgc cggacagcgg cgcgctgaac
     7261 ggcaccggtt ccccgtcgac ccggatgtac tgctcgctgt ggaacgacgc ggcggcgtgc
     7321 cgggtgtcga ccgagacctt gcccggctcg attccgcgca gccgcaacag ttccccggcg
     7381 gccagcgtcg ccgcggcgat actcgtcgtc gccgcctcct cgacccggaa ggtcccgggc
     7441 agcaccgaat cggggccggt gatctcgacg gcgtcttcgg cgagggtctc gccggtgagc
     7501 gtggtccaga cgcgcgtgtc cacggaggcc ttcactaggg caggagcgcg atcagctcgg
     7561 ccggttccac cttggtcccg gtgtagaacg ggatctcctc ccgcacgtgg aggcgggcct
     7621 cggtgccgcg cagatgccgc atgaggtcga cgatgcggtg cagttcgtcg gcctcgaagg
     7681 ccaggatcca ctcgtagtcg cccagcgcga acgacgcgac cgtgttggcg cggacgtccg
     7741 ggtagtcgcg ggcctccttg ccgtggtcgg cgagcatctt gcggcgctcg tcgtccggca
     7801 gcaggtacca ctcgtaggag cgcacgaacg ggtagacgca gatgaacttg cgggcctctt
     7861 caccggcgag gaacgccgga atgtggctct tgttgaactc cgccgggcgg tgcagcgcga
     7921 cctggctcca caccggcgcc gacgcgcggc cgagcggagt gtgccggaag ccgctgtagg
     7981 ccgcctggac ctgctcgatc tcctcggcgt gccaccagat catgtagtcg gcgtcggcgc
     8041 gcagccccga gaggtcgtag acgccgcgga cgacgacgcc cttggcctcc agggcgtcga
     8101 ggtactcggt ggtctccgcg gtcgccttcg cgcggtcttc gccgagcttg ccctgctcgg
     8161 cccggaagac cgaccaggcg gtgtagcgga tggtgtcatt gagctcgttg tagttcagcc
     8221 gcgccatacc gccatcttgg cactgccggt gctcaggagc cctcgtgggt cggtgtgatc
     8281 ccggcgatgc ggcgggcggc cgcgtcggcg gtggcgatgc aggccgggat cccgacgccg
     8341 tgcagcgtcg cgcccgccac ggcgaggccg gggacggcgg cgacggcccg ttcgatccgc
     8401 tcgacacgga cgaggtgccc gatgccgtac tgcgggagcc cgccgcccca gcgggtcacc
     8461 agggtgtcga tcggctcggc ggtgaccccg gtcagccggg ccaggtcgtc gcggaccacg
     8521 cgcacgagtt cgtcgtcgtc ggcgtgcagc gctcccggtt cgccgaagcg gccgaccgag
     8581 ccgcgcacca gcaccgggcc ttcgccgtac tgcgcccact tgcgggagga gaaggtgaac
     8641 gccttggcgg cgaacggctt cccggccgca tcccgctccc ctgcgccgat cagcacgccg
     8701 gacgactccg gcaacgcggt ccccggcgga agcgcgagcg cgaccaccgc catcgacgcg
     8761 agttcgatct tggcgaacac cgtcgaggcc acttccgcga caccgtcgag cagccgtcgt
     8821 gccgaaggcg ccgggacggc gaggagaacg gagtcggcgt cgaggtcgcc gaccttccag
     8881 ccggtggggg tgcgctcgag cgccgtcacc gtggtcccgg tccggatctc ggcgcgggac
     8941 agctccgcga gccgggcgat cagtgttccg agtccgccca gcagggtgcc gaagaccggc
     9001 gccgtactcg gcgaggcggg catgagcgag gcggcgcctt cggtgagcga gcgcgcgccg
     9061 cgatcgatcg cggacgcgag accgggcatg gtcgcgcgca gcccgaggcc gtccgctccc
     9121 ccggcgtaga cgccgcccag cagcgggtcg acgagccggt ccacgagttc gtcgccgaac
     9181 cgctcacgca gcagctcgcc gagcggcacg tcctccgcgt gcagtttcag cggaggcagc
     9241 gaagcctcgg cctcgaccag ggcacgcccc ttctccgaca gcacccccgc gaccgcgtcg
     9301 gccgaggccg gaacacccat gaccgtgccc ggcggcaggc cgagcacgga gccgccggcg
     9361 tggatcttgg cgcgggcctt ggtgggatgc acgaggcttt cgtccagccc gacctcgcgg
     9421 accaaggcga gcgcttcggg gcggcgggcg aggaaggcct ccgcgccgac gtcgtagcgt
     9481 tcaccggcga attccaccgt gcggagcttc ccgccgagcg agtcggtcgc ctcgaagacg
     9541 acgatccggg cggcttcgcc gagcagcgtc cgcagccggt acgccgcggt cagtcccgag
     9601 ataccgccac cgacgacggc gacggtcttc acggctggag ctcgtgcacc agcccgacca
     9661 cgcgtgtcag cacgtccggg tcgacgccgg gcagcacgcc gtggccgagg ttgaagatgt
     9721 ggccgtcggc cgcgcggccc tcggcgtgga tgcgccggac ctccgcttcg agcaccggcc
     9781 aggccgcgtg gagcagcgcc gggtcgaggt tgccctggac gacaccgccg ccgagccgcc
     9841 ggacggcttc gtcgagcggg atgcgccagt cgacgccgac cacgtcggca cccgcgtcgc
     9901 gcatggcgac cagcagctcg ccggtgccga cgccgaagtg gatccggggt acgccgtagc
     9961 ccgcgaccgc ggacagcacc ttggccgaat gcggcaggac gaactcgcgg tagtcccgct
    10021 cagagagcgc gcccgcccac gagtcgaaca gctggatcgc gtcggccccg gcgtcgagct
    10081 gcgcggagag ggaggtgatc gccatgtccg cgagacgccc ggccagctcg tgccacagct
    10141 ccggctcgga gtgcatgagc gccttggtgt gctcgtggtt gcggctcggc ccgccctcga
    10201 tgagatacga ggccagggtg aacggcgcgc ccgcgaaccc gatcagcggc gtctcgccca
    10261 gcgacccgac cagcagccgg accccttcgg cgacgcgctc cacctgctcc ggttccagca
    10321 ccggcagcgc acgcacgccc gcggcgtcgc ggatcggctc ggcgacgacc gggccggtgc
    10381 ccgcgacgat gtcgatgtcg aggcccgccg ccttgagcgg gaccacgatg tcactgaaga
    10441 ggatggccgc gtcgacgccg tgacggcgga tcggctggag cgtgatctcg gcgagcatct
    10501 cggggtcgaa gcaggcttcg aacatcgagg tgccctcgcg cagcgcccgg tactcgggca
    10561 gcgaccggcc cgcctggcgc atgaaccaca cgggggtgtg agccgggcgt tcgccgcgcg
    10621 cggcggccag gaacggcgca ccgggcaggg cgcgacgggc cgcggggacg gtctgcgcgg
    10681 gagaagcaac tgaaggagac atcacggttc atggtgccat gtgcgctcag gggccttact
    10741 ctcggcgcgc ctgcgccccg aaccggacga tcgcgtctta cagtcggttg gtgaccgcga
    10801 tgacgtctgt acccga
//
