LOCUS       NZ_CP024972            10390 bp    DNA     circular CON 18-AUG-2024
DEFINITION  Amycolatopsis sp. TNS106 chromosome, complete genome.
ACCESSION   NZ_CP024972
VERSION     NZ_CP024972.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. TNS106
  ORGANISM  Amycolatopsis sp. TNS106
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 8806031)
  AUTHORS   Liu,K. and Tao,M.
  TITLE     Discovery of new natural product by Amycolatopsis speibonae genome
            mining
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8806031)
  AUTHORS   Liu,K. and Tao,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, Shanghai
            200030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP Assembly v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 116.0x
            Sequencing Technology             :: Illumina HiSeq 4000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019330265.1-RS_2024_08_18
            Annotation Date                   :: 08/18/2024 03:49:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,321
            CDSs (total)                      :: 8,231
            Genes (coding)                    :: 8,179
            CDSs (with protein)               :: 8,179
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 20 of 52
            Pseudo Genes (incomplete)         :: 35 of 52
            Pseudo Genes (internal stop)      :: 4 of 52
            Pseudo Genes (multiple problems)  :: 6 of 52
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:02:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2591580
            Orig. end                         :: 2601970
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024972.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="15"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5390](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5390)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(180..189)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.12"
                     /tool="antismash"
     gene            complement(450..2129)
                     /gene="ilvD"
                     /locus_tag="CVV72_RS11700"
                     /old_locus_tag="CVV72_11620"
     CDS             complement(450..2129)
                     /EC_number="4.2.1.9"
                     /GO_function="GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="ilvD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005156871.1"
                     /locus_tag="CVV72_RS11700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_11620"
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="WP_192744576.1"
                     /transl_table=11
                     /translation="MPQDQPDLKPRSRDVTDGLERAAARGMLRAVGMGDDDFAKPQIGV
                     ASSWNEITPCNLSLDRLAKAVKNGVHAGGGYPLEFGTISVSDGISMGHEGMHFSLVSRE
                     VIADSVETVMMAERLDGSVLLAGCDKSLPGMLMAAARLDLASVFLYAGSIMPGQVDGQD
                     VTIIDAFEAVGACLAGKITRAEVDRIERAICPGEGACGGMYTANTMASVAEALGMSLPG
                     SAAPPAVDRRRDGFAHRSGEAVVGMLRQGITARQIMTMEAFENAIAVVMALGGSTNAVL
                     HLLAIAREAEVDLRIDDFNRIGDKVPHLGDLKPFGKYVMNDVDKVGGIPVVMKALLDAG
                     LMHGDVLTVTGKTMAENLEGIAPAGVDGEIIRPLDRPIHKTGGLTILKGSLAPEGAVVK
                     SAGFDESTFTGTARVFDGERAALDALAEGRIVAGDVVVIRYEGPKGGPGMREMLAITGA
                     IKGAGLGKDVLLITDGRFSGGTTGLCVGHIAPEAVDAGPIAFVRDGDPITLDVANRTLE
                     VEIDDIEARKEGWTPKPPAYTRGVLGKYAKVVRSAAHGAVCE"
     gene            complement(2193..3473)
                     /locus_tag="CVV72_RS11705"
                     /old_locus_tag="CVV72_11625"
     CDS             complement(2193..3473)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1273: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636378.1"
                     /locus_tag="CVV72_RS11705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_11625"
                     /product="hemolysin family protein"
                     /protein_id="WP_219152459.1"
                     /transl_table=11
                     /translation="MGGYGFDIALVAVLVVLNAVFAGSEMALISLREGQLRALERDGRA
                     AARTLVRLARDPNRFLATIQIGITLAGFLASATAAVSLAQPLVPLLDFLGDAAGAVAVA
                     LVTVVLTFLTLVLGELAPKRLAMQNAVRWALLVARPLNLLSAISRPVVWALSVSTNFVV
                     RVLGGRAEADPDQMSPEELRELVSAQRGLNAEQRMIINGALEIHERRLREVLVPRRAVL
                     TLAAEQDIESARRQLAESGHSRAPVARGGHLDDVVGVVQLRDLLGDHEDLAEAVRPAVV
                     FPDSLRVSDALRSFKAEREQMALVVDEHGAVAGMVTLEDLLEEIVGEIYDETDRDVLAV
                     RDGTDGALVLPGTFPVHDLVDVGVELRDAPPGEYATIAGLILVILGRIPEKPGDRVTVS
                     GWTAEVLGVEHHAITRVALHRGPNAER"
     gene            3605..4939
                     /locus_tag="CVV72_RS11710"
                     /old_locus_tag="CVV72_11630"
     CDS             3605..4939
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029100.1"
                     /locus_tag="CVV72_RS11710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_11630"
                     /product="cytochrome P450"
                     /protein_id="WP_219152460.1"
                     /sec_met_domain="p450 (E-value: 5.3e-27, bitscore: 93.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFALKTAPARTFRHKAEAIAKPVTRWSIGHALPRVALRAAARRGD
                     LQGRLSVEASGGADLSGLFDEIRAHGAIATTRVGHVSTRHSACKEVLTSDAFKTVRFVP
                     DNPLLNRLVAWSASGLIHPIEPPSLLASEPPDHTRYRKLVTRVFTARAVEQLRERTQAI
                     TDELLDGLAPANPVDLVEHYCGLLPVTVISQILGVPPEERDKVLALGGAAAPSLDLGLP
                     WRTFRTVEATLAEFDSWLTLHLEKLRENPGDNLLSKLIAAREDGIGLTERELKATAGLV
                     LAAGFETTVNLLGNGIALLTRHPGELAKLRANPELWSNAVDEILRYDPPVLLTGRLSAR
                     DTEIGGARIERGAIVTTVLAGANRDPEIFDDPATFDVERANARDHVSFGAGRHYCLGAS
                     LARMEGEIGLRSIFERFPDLRLLDGARRRETRILRGFETLPAALS"
     gene            complement(5001..5390)
                     /locus_tag="CVV72_RS11715"
                     /old_locus_tag="CVV72_11635"
     CDS             complement(5001..5390)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="CVV72_RS11715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_11635"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_219152461.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 5.5e-12,
                     bitscore: 45.0, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKILLGAVLGSSLMVASLFATAPMASAGPAEGVSVVLASSGAQGV
                     VLSGSVPFACQNISGGTWCYGTESAGSGQKKCYSHYLHPTKRHSATAILGSRQSKKVAN
                     RGQWAKAEVIGSRNDTCNAYYNSDA"
     misc_feature    complement(5382..5396)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.17"
                     /tool="antismash"
     misc_feature    5392..5406
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.84"
                     /tool="antismash"
     misc_feature    complement(5408..5423)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.48"
                     /tool="antismash"
     gene            5642..6634
                     /locus_tag="CVV72_RS11720"
                     /old_locus_tag="CVV72_11640"
     CDS             5642..6634
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /locus_tag="CVV72_RS11720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_11640"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_219152462.1"
                     /transl_table=11
                     /translation="MLRIHFTLADLAGIRIARRPDPLWEVLMSVYLLRKTRAEIVLGGW
                     RSQTRQRLTPSTRPLLDLVPASGSSPDFLTPPGGGTLSERIDSLLSTPRSRLTADFAHL
                     ARERPLPSWTVDLAEGRPPALRMVAHALRGYFHACLEPHWDAIATAVRHDHTRRAQLMA
                     DQGIDAVLSTLSPAATWRFPVLSIDYLFDQDVRLDGRGLLLQPVVFGRGPNPTTLIDPA
                     LPPVLVYSVPLAPGVITPAEPARRRAADPIVALLGTTRTRILRALDTTEATTTELARRA
                     ATPLPTASRHATALRDANLITSHRHKNMMIHAITPLGTALLRGRQPHLN"
     misc_feature    5735..5737
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    6602..6604
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6914..7318
                     /locus_tag="CVV72_RS11725"
                     /old_locus_tag="CVV72_11645"
     CDS             6914..7318
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636380.1"
                     /locus_tag="CVV72_RS11725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_11645"
                     /product="hypothetical protein"
                     /protein_id="WP_255632639.1"
                     /transl_table=11
                     /translation="MTILILIGPALLWISTYFDPGDDDRLVVIAMGAVFTLGGVGTVGS
                     HLWQNAKAREAHRGLDRSGVRANAEVVSRKRIWVGEVSTPADDVDLVVTGPGVTPFSAR
                     VLTEDVGRYETGTVVPVDVDPRTLLFRLAD"
     gene            7400..8380
                     /locus_tag="CVV72_RS11730"
                     /old_locus_tag="CVV72_11650"
     CDS             7400..8380
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1015: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333944.1"
                     /locus_tag="CVV72_RS11730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_11650"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_034306187.1"
                     /transl_table=11
                     /translation="MTTATADPTTTTVARDFTDFRTVVSQSFVPLHVTSEHRDHFRGRI
                     RSCGADDVQLTEVTASAHVVERTPELIARADRHYYKLSLILSGTGLLVQDNREAMLRPG
                     DVALYDTHRPYTLAFEEDFRTLVVMFPQRLIDLPRDLVGQLTAVPMSGKRGMGNVVVPF
                     LAQLGSNLDQLGGPAGVRLAHTAVDLLATLFASELDLARATAGPHHELMRRVLAYIDSN
                     LSSTDLGPAQIAAEHYISTRHLHGLFHEQGTTVSGWIRTRRLEHCRRELLDPVHHSRPV
                     AAIAAKWGFVDAPHFSRVFKTEFGCSPSELRRGLTVSDRLLPPSA"
     gene            8421..9188
                     /locus_tag="CVV72_RS11735"
                     /old_locus_tag="CVV72_11655"
     CDS             8421..9188
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333943.1"
                     /locus_tag="CVV72_RS11735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_11655"
                     /product="MoaF C-terminal domain-containing protein"
                     /protein_id="WP_219152464.1"
                     /transl_table=11
                     /translation="MPEAPEEQWRTYDEFAAGIATYRLPNADLSGRELTVTLDDGKTLA
                     LRFEDAETVSCNGIKDPYDAVAVRDDVFFVNLPHTSVEGEALTVVFSTTTHRALAVRSV
                     IGAKDVEGVPRVSQTFWSGTTDAGTPTGEVPGPSRDLIGKRNIYRYSPEHLYEHVYVSS
                     QRYAWQCLEGVQRGHGDMDLSTVWKFQDGLYLFCFREFRIAVASVWLHDLGYALKTTGV
                     FLGLTGDGRSEHSRAGGHIYPLGAVAYPDAQPV"
     gene            9198..9599
                     /locus_tag="CVV72_RS11740"
                     /old_locus_tag="CVV72_11660"
     CDS             9198..9599
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636383.1"
                     /locus_tag="CVV72_RS11740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CVV72_11660"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_219152465.1"
                     /transl_table=11
                     /translation="MSNADIIAAHYAASDRGDLAGMLAPLGPETTWTEAAGFPYAGTYT
                     GPEEVRDNVFGAIAKDWDGYTFTLDDLLDAGDTVIGLGTYRGKHRETGRSFTARVAHVW
                     KFDGGKVASFEQIVDSVPVVDAMNASENR"
ORIGIN
        1 gtgaaggcct ccttgcctac cctggtggca tttcgaaggt cgtatgtggc gacctgaatc
       61 tggactcgcc gacgagagcc gaccgcagcc tgagggtcca caaaggacac catttccggt
      121 cacagggccc gatttgtgca ctttgcgcga ggtcgtgagt gttttgggtc gttagaacgg
      181 cacaaaacac tcacgaccct cccgcaaact gtgcaaatcg acatcaaccg gacaccgggc
      241 cgccaggacg tgccccctgt ggacactcag cacccgagcg gtacgtagcc gtagccccgg
      301 acgaaggacg gtgatcccga cgggtgccgg aatcaccgct tacgcccctg aacagcggtt
      361 atgcgacttc gccgggttcc tgccctgaag gtaggcgaga gtcccctcac gccccctcgt
      421 caaatcgccg cccgcgcatc gggcaggtct cactcgcaaa cggctccgtg cgcggcgctg
      481 cggaccacct tggcgtactt gccgagcact cctcgcgtgt acgccggcgg cttgggggtc
      541 cagccctcct tgcgcgcctc gatgtcgtcg atctcgactt cgagggtccg gttcgcgacg
      601 tcgagggtga tcgggtcacc gtcccgcacg aacgcgatcg gcccggcgtc gacggcttcc
      661 ggggcgatat gcccgacaca cagcccggtc gtgccgccgc tgaaccggcc gtcggtgatg
      721 agcaggacgt cctttccgag cccggcgccc ttgatcgccc ccgtgatggc cagcatctcc
      781 cgcatccccg gcccgccctt gggcccttcg taccggatga cgacgacgtc cccggcgacg
      841 atccggcctt ccgcgagcgc gtcgagggcg gctcgctcgc cgtcgaacac ccgcgcggtg
      901 ccggtgaacg tcgattcgtc gaagcccgcc gacttcacga ccgcgccttc gggtgccagc
      961 gatcccttga ggatggtcag cccgccggtc ttgtggatcg ggcggtcgag cggacggatg
     1021 atctcgccgt cgaccccggc cggggcgatg ccttcgaggt tctcggccat ggtcttgccg
     1081 gtcacggtga gcacgtcgcc gtgcatcagt ccggcgtcga gcagcgcctt catcaccacg
     1141 gggatgccgc cgaccttgtc gacgtcgttc atcacgtact tcccgaacgg cttgaggtcg
     1201 ccgagatgcg ggaccttgtc cccgatccgg ttgaaatcgt cgatgcgcag gtcgacctcc
     1261 gcctcgcgcg cgatcgcgag caggtggagg acggcgttgg tcgaaccgcc cagcgccatc
     1321 acgacggcga tggcgttctc gaacgcttcc atggtcatga tctggcgcgc ggtgatgccc
     1381 tggcgcagca tcccgacgac ggcctccccc gaccggtggg cgaacccgtc acgacgccgg
     1441 tcgacggcgg gcggagcggc cgacccgggc agcgacatac cgagggcctc ggcgaccgac
     1501 gccatcgtgt tggcggtgta catcccgccg caggcgcctt cgccggggca gatcgcgcgt
     1561 tcgatcctgt ccacttcggc gcgggtgatc ttccccgcca gacaagcgcc gacagcctcg
     1621 aacgcgtcga tgatggtgac gtcctggccg tccacctgac cgggcatgat cgagcccgcg
     1681 tagaggaaaa cggaagcgag atcgaggcgc gccgccgcca tcagcatgcc cggcagggat
     1741 ttgtcgcagc ccgccagcag cacggagccg tccagccgtt cggccatcat caccgtctcg
     1801 accgaatcgg cgatcacctc acgggacacc agcgagaagt gcatgccctc gtggcccatg
     1861 gagatgccgt cggacaccga gatcgtgccg aactcgagcg ggtagccacc gcccgcgtgc
     1921 acgccgttct tgaccgcctt ggccagccgg tcgagcgaca ggttgcacgg cgtgatctcg
     1981 ttccacgagg acgccacccc gatctgcggt ttggcgaagt cgtcgtcgcc catcccgacc
     2041 gcgcggagca tgccgcgggc cgccgcacgc tccaggccgt cggtcacgtc gcgtgaacga
     2101 ggtttcaaat ccggctggtc ttgcggcatc ggaacacctt tcttcgtggc gggacaccgg
     2161 gcacgctgcc aacccggcgc gtccggcgca actcaccgct ccgcgttcgg tccacggtgc
     2221 agcgccaccc gcgtgatcgc gtgatgctcc acgccgagta cttcggcggt ccagccggac
     2281 accgtcaccc ggtcgcccgg cttttccggg atccggccga ggatcacgag gatcagtccc
     2341 gcgatcgtcg cgtactcccc cggcggcgcg tcgcgcagtt ccaccccgac gtcgacgagg
     2401 tcgtgcaccg ggaaggtgcc gggcaggacc agcgcgccgt ccgtaccgtc gcggacggcc
     2461 agtacatcgc ggtcggtctc gtcgtagatc tcgccgacga tctcctccag caggtcctcc
     2521 agcgtgacca tgcccgcgac ggcgccgtgt tcgtcgacga cgagcgccat ctgctcccgc
     2581 tcggccttga aactccgcag cgcgtccgac acccgcagcg aatcggggaa cacgaccgcc
     2641 ggccggacgg cctcggccag gtcttcgtgg tcgccgagca gatcacgcag ctgtacgaca
     2701 ccgacgacgt cgtccagatg gccgccgcgg gcgaccggcg cccgggaatg gccggactcg
     2761 gccagctgcc gccgcgccga ctcgatgtcc tgctcggccg cgagcgtcag caccgcccgg
     2821 cgagggacga gcacctcccg cagccgtcgc tcgtggatct ccaaggcacc gttgatgatc
     2881 atgcgctgct cggcgttcag gccgcgttgc gccgagacca gttccctcag ttcctcgggc
     2941 gacatctgat ccggatcggc ttccgcccgg ccgcccagca cccgcacgac gaagttcgtc
     3001 gacacgctca gcgcccacac cacgggcctc gagatcgccg acaacagatt cagcggacgc
     3061 gccaccagca acgcccaccg caccgcgttc tgcatggcga gccgtttcgg ggcgagttcc
     3121 ccgagcacca aggtcaggaa cgtgaggacc accgtcacca gcgcgacggc caccgcaccg
     3181 gccgcgtcgc cgaggaaatc cagcaacggc accaggggtt gcgcgagcga caccgccgcc
     3241 gtcgccgacg cgaggaaacc ggcgagcgtg atcccgatct ggatcgtcgc caggaaccga
     3301 ttggggtcgc gcgccagccg taccagcgtg cgggcggccg cgcgaccgtc gcgctccaac
     3361 gcccgcaatt gcccctcacg caacgagatc agtgccatct cgctgcccgc gaacaccgcg
     3421 ttgagcacca ccagcaccgc gaccagcgcg atgtcgaacc cgtagccgcc cacaagcact
     3481 ccccggcccg cccgaagtgc agaacactac cgagcgggca gcgcgtccgc agaaatcgga
     3541 gccgctggct actgacacgt cgttccgata ccttcgagag ggaacccgca cggacggagt
     3601 cctgatgttc gcactcaaga ccgctcccgc acggaccttc cggcacaagg cggaggcgat
     3661 cgccaaaccg gtcacccgct ggagcatcgg ccacgccctc ccccgggtcg ccctccgggc
     3721 cgcggcacgc cgcggcgacc tccagggcag gctgtccgtc gaggcgagcg gcggggcgga
     3781 cctgagcggg ctgttcgacg agatccgcgc gcacggcgcc atcgccacca cccgggtcgg
     3841 ccacgtcagc acccggcatt cggcgtgcaa ggaggtgctg accagcgacg cgttcaagac
     3901 cgtacggttc gtcccggaca acccgctgct caaccggctg gtcgcgtggt ctgcgtccgg
     3961 cctcatccat ccgatcgagc cgccgtcgct gctcgccagc gaaccgcccg accacacgcg
     4021 ctaccggaag ctggtcaccc gcgtcttcac cgcccgggcg gtcgagcagc tgcgcgagcg
     4081 cacgcaggcg atcaccgacg aactgctcga cggcctcgcc ccggcgaacc ccgtcgacct
     4141 cgtcgagcac tactgcgggc tcttgccggt caccgtgatc agccagatcc tcggcgtccc
     4201 gccggaggaa cgggacaagg tgctcgcgct gggcggcgcg gcggccccga gcctcgacct
     4261 cggcctgcca tggcggacct tccgcacggt ggaggcgacg ctggccgagt tcgactcatg
     4321 gctcaccctg catctggaga aactgcggga gaaccccggc gacaacctgc tcagcaagct
     4381 gatcgccgcc cgcgaggacg ggatcggcct gaccgagcgg gaactcaagg ccaccgccgg
     4441 gctggtcctg gcggccggct tcgaaacgac cgtcaacctc ctcggcaacg gcatcgccct
     4501 gctcacccgg catccgggcg aactcgcgaa actgcgggcg aatccggagc tgtggagcaa
     4561 cgccgtcgac gagatcctcc gctacgaccc gccggtcctg ctcaccggca ggctgtccgc
     4621 gcgggacacc gagatcggcg gtgcccgcat cgagcgcggc gcgatcgtca ccaccgtgct
     4681 cgcgggcgcc aaccgggacc cggagatctt cgacgatccc gccaccttcg acgtcgagcg
     4741 ggcgaacgcg cgggatcacg tgtcgttcgg cgcggggcgg cactactgtc tcggcgcctc
     4801 gctggcgagg atggaaggcg agatcgggct gcggtcgatc ttcgagcgct tcccggatct
     4861 gcgcttgctc gacggcgcgc gacggcggga gacccggatc ctgcgcggct tcgagacgct
     4921 ccccgccgcg ctcagctgac cccaccttcg cggggcgccg aacccggcac ggccggatcc
     4981 ggcgccccgc gtcggtcacc tcacgcgtcg gagttgtagt acgcgttgca ggtgtcgttc
     5041 cgggaaccga tcacctcggc cttggcccac tgcccgcgat tcgcgacctt cttgctttgc
     5101 ctggagccca gaatggcggt cgcgctgtgg cgcttggtgg ggtgcaggta gtgcgagtag
     5161 cacttcttct ggccgctgcc cgccgattcg gtcccgtaac accacgtgcc gccgctgatg
     5221 ttctggcagg cgaacggcac cgagccgctc agcaccacac cttgggcacc cgaggacgcc
     5281 agcaccacgg agacgccctc cgccgggccc gcggaggcca tcggcgccgt cgcgaacaga
     5341 cttgccacca tcaaactcga tcccagtacc gctcccagca gaattttcat gatcatcctt
     5401 cacgatggac attgattgtc tttggcgaga taaccggtaa ggcgatttac ccccactaac
     5461 gaattcgacg ccaatactag gacggctcaa cagaagcgga cagtgtcttc actgtgccga
     5521 aaacatgcag atcagcggcc gattcccgag tatcaggcgc cgccgggcgg aaccgcggca
     5581 cgaccgcggt ggcaacgatc atgtttccgc agtagcgtcg caggcggggg aggaagaggg
     5641 aatgctgcgg atccatttca cactcgcgga cctggccggt atccggatag cgcgacgccc
     5701 ggatccgttg tgggaggtcc tgatgagtgt gtatttactg cgcaaaaccc gggcggagat
     5761 cgtgctgggc ggctggagaa gccagaccag gcagcggctg acaccgtcca cgcggccgtt
     5821 gctggatctg gttcccgcgt caggctcctc gcccgacttc ctgaccccgc ccggcggggg
     5881 caccctgtcc gagcgcatcg actcgctgct gagcacgcca cgctcccggt tgacggccga
     5941 cttcgcccat ctggcgcggg aacggccgct tccttcttgg accgtcgatc tcgcggaagg
     6001 gcgaccaccc gcgttgcgca tggtcgccca cgccctgcgg ggatacttcc acgcctgcct
     6061 tgagccgcac tgggatgcga tcgccaccgc cgtgcgacac gatcacaccc gccgcgccca
     6121 gctcatggcc gaccagggca tcgacgccgt cctgagcacc ctgagccccg cggcgacgtg
     6181 gcgctttccc gtgctgagca tcgactatct cttcgaccaa gacgtccgcc tcgacggacg
     6241 gggcctgctg ctgcagccgg tggtcttcgg ccgcggcccc aaccccacca ccctgatcga
     6301 cccggcgctt ccgcccgtcc tggtctattc ggtgccgctc gcccctgggg tcatcacccc
     6361 ggcggaaccg gcccgacgac gcgccgccga tccgatcgtc gccctgctcg ggaccacccg
     6421 cacccgtatc ctgcgcgctc tcgacaccac cgaggccacc accaccgaac tggcccgccg
     6481 ggcagccacg ccgctaccca ccgccagccg ccacgccacc gcgctccgtg acgccaacct
     6541 gatcaccagt caccgccaca agaacatgat gatccacgcg atcaccccgc tcggcaccgc
     6601 gttactccgc ggacggcaac cgcacctgaa ctgacgcacc ggtcaccgag ccgggtcttt
     6661 cagttcactc accgccgggt gatcggctcg gagtacacaa aggacctcgc cagtactggt
     6721 gaaatcccgc gcgttcgagc ggaacgatcc ttcgacgcgc tcgcccggca caacggcggg
     6781 caagcgggca tttttaccgg accagcgacg gaaaccggtc agtaatctca cgcccgtgaa
     6841 gacggaccgc gagggcaggg aggcaccgga gctccctgcc ttcaccgtca tcggatgcct
     6901 tttgggcttc ttcatgacca ttctgatcct gatcggcccc gctctgctct ggatcagcac
     6961 ctacttcgac cccggcgacg acgaccgcct ggtcgtcatc gccatgggcg ccgtgttcac
     7021 gctcggcggt gtcggcaccg tcggctccca cctctggcag aacgccaagg ctcgggaggc
     7081 ccatcgcggg ctcgaccggt cgggggtccg cgcgaacgcc gaagtcgttt ccaggaaacg
     7141 gatctgggtc ggcgaggtga gcacgcccgc ggacgacgtc gatctcgtcg tcaccggccc
     7201 gggggtgact ccgttcagtg cgcgcgtcct caccgaggac gtcggccgtt acgaaacggg
     7261 cacggtggta cccgtcgacg tggacccgag gaccctcctg ttccgtctcg ccgactgacc
     7321 tcgccgcgat cattgcccgg cagcatcgta tcgcttatcg tatatgaaat tcaccccccg
     7381 atgctgcgag gtgacccccg tgaccaccgc caccgccgac ccgaccacca ccacggtcgc
     7441 gcgagatttc accgatttcc gcacggtggt gtcgcaatcc ttcgtgccac tgcacgtgac
     7501 gagcgagcac cgggaccact tccgcggccg catccgctcg tgcggcgcgg acgacgtcca
     7561 gctgaccgag gtgaccgcgt ccgcccacgt ggtcgagcgg acgcccgagc tcatcgccag
     7621 agcggaccgg cactactaca agctgagcct catcctgtcc gggaccgggc tgttggtgca
     7681 ggacaaccgc gaggcgatgc tccgtcccgg agacgtggcg ttgtacgaca cgcaccggcc
     7741 gtacacgttg gcgttcgagg aggacttccg cacgctggtg gtgatgttcc cgcagcggct
     7801 catcgatctc ccccgtgacc tggtcggcca gctgaccgcg gtgcccatgt cgggcaagcg
     7861 gggcatgggc aacgtggtgg tgcccttcct cgcgcaactc ggcagcaacc tcgaccagct
     7921 cggcgggccg gcgggcgtcc ggctcgcgca tacggcggtc gatctgctcg ccacgctgtt
     7981 cgccagcgaa ctggacctcg cccgcgcgac cgcggggccg caccacgagc tgatgcgccg
     8041 cgtgctcgcc tacatcgact cgaacctctc ctccaccgac ctcggcccgg cgcagatcgc
     8101 cgccgagcac tacatctcga cccggcatct gcacggcctc ttccacgaac agggcacgac
     8161 cgtctcgggc tggatccgca cccggcggct cgaacactgc cgccgcgaat tgctcgaccc
     8221 cgtgcaccat tcgcggcccg tcgcggccat cgcggcgaag tggggtttcg tcgacgcgcc
     8281 gcatttcagc cgggtgttca agacggaatt cggctgctcg ccgagcgaac tgcgccgcgg
     8341 cttgaccgtg agcgaccggt tgttgccgcc cagcgcgtga cagctggcct cgccggtgcg
     8401 gtcgcctcga cgatgaccgc atgcccgaag cacccgagga acagtggcgc acctatgacg
     8461 agttcgccgc cggaatagcc acctaccgcc tgccgaacgc ggacctgtcg ggacgcgagc
     8521 tcaccgtcac cctcgacgac ggaaagacgc tcgcgctgcg gttcgaagac gctgaaaccg
     8581 tctcctgcaa cggcatcaag gacccgtacg acgcggtcgc cgtccgggac gacgtcttct
     8641 tcgtcaatct cccacacacc agcgtcgaag gtgaagcgct caccgtcgtc ttctccacga
     8701 cgacgcatcg cgcgctcgcc gtgcgctcgg tgatcggcgc caaggacgtc gaaggggttc
     8761 cccgtgtctc ccagacgttc tggtccggca cgaccgacgc gggtacgccg accggcgagg
     8821 ttcccggccc gtcgcgcgac ctgatcggca agcgcaacat ctaccgctac agccccgaac
     8881 acctttacga gcacgtgtac gtgtcctcgc aacgctatgc ctggcaatgc ctcgaaggtg
     8941 tgcagcgcgg tcacggggac atggacctgt cgacggtgtg gaagttccag gacgggctct
     9001 acctgttctg tttccgggaa ttccgcatcg ccgtggccag cgtgtggttg cacgacctcg
     9061 gctacgcgct gaagaccacc ggcgtcttcc tcggcctgac cggcgacggc cggtcggaac
     9121 actcccgcgc gggcgggcac atctacccac tcggcgccgt cgcctatccc gacgcgcaac
     9181 cggtttaagg agtctcagtg tccaatgcag acatcatcgc cgcgcactac gccgcgagcg
     9241 accggggcga tctggcgggc atgctggccc cgctcggtcc ggagaccacg tggacggagg
     9301 cggccggttt cccctacgcc ggaacctaca ccggccccga agaagtccgg gacaacgtct
     9361 tcggcgcgat cgcgaaggac tgggacggct acaccttcac cctcgacgac cttctcgacg
     9421 ccggtgacac ggtgatcggc ctcggtacct accgcggcaa gcaccgcgag accggtcgct
     9481 cgttcaccgc ccgcgtcgcg cacgtgtgga agttcgacgg cggcaaggtc gcttcgttcg
     9541 agcagatcgt cgactcggtc cccgtggtcg acgccatgaa cgcttcggag aaccggtgaa
     9601 acccgcgcgg cgcctcgtcg ccctcgccgc cgccgtcctg ctcggcaccg cggcctgcgc
     9661 cggcgccgaa agcggttcga gcgggccgat cgtggtcggt tcggtcaatg ccctgagcgg
     9721 cgcggccacg ttccccgaag cctcgcaggc ggccaaggcg gtcttcgacg cggcgaacgc
     9781 gagcggcggc gtgaacggca gacagatcca gtacaaggcc ctcgacgaca agggcgaccc
     9841 ggcggccgcg gccgccgcgg cccgcgaggt cgtcggccgt gacgaggcgg tggcgctcgt
     9901 cggctcgtcc agcctcatcg agtgcgagat caacaacaag tactacgaac agcagaagat
     9961 cctgtccatg cagggtatcg gcgtggaccc ggcctgtttc tccagcccga acatcgcccc
    10021 ggtcaacgtc ggcccgtacc acgacatgac gctgaccctg ctctacggct cggaaacgct
    10081 gaagctgaac gacatctgcg cgctgctgga gatcgcgggc aacacgctgc cgtcgtacca
    10141 ggccgcgatc gacgagtggt cggccatcac cggcaagaag ctcaagtacc tcgacgcgac
    10201 cgtgccctac ggcggttcgg actacacctc ctacatcgtc aaggcacgca acgccgggtg
    10261 caaggcgatc acggtgaacc cggtcgagcc ggattccatc gggcagctca aggccgccgc
    10321 ggcacagggc tggaacgacg tcacgtggct gctgctcacc agcgtctaca gcgagaacta
    10381 cgccaaggcc
//
