LOCUS       NZ_JAEVFI010000056      2199 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Streptomyces sp. JJ66 scaffold56_size2199, whole genome shotgun
            sequence.
ACCESSION   NZ_JAEVFI010000056
VERSION     NZ_JAEVFI010000056.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. JJ66
  ORGANISM  Streptomyces sp. JJ66
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Jose,P.A. and Jha,B.
  TITLE     Draft genome of Streptomyces sp. JJ66
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Jose,P.A. and Jha,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2021) Marine Biotechnology and Ecology Division,
            CSIR - Central Salt and Marine Chemicals Research Institute, G.B.
            Marg, Bhavnagar, Gujarat 364002, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-MAY-2017
            Assembly Method                   :: Velvet v. V 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 320.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019303495.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 03:51:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,446
            Genes (coding)                    :: 4,340
            CDSs (with protein)               :: 4,340
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 61
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 31 of 106
            Pseudo Genes (incomplete)         :: 82 of 106
            Pseudo Genes (internal stop)      :: 3 of 106
            Pseudo Genes (multiple problems)  :: 9 of 106
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:22:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2199
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAEVFI010000056.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2199
                     /collected_by="Polpass Arul Jose"
                     /collection_date="2015-10-14"
                     /db_xref="taxon:2803843"
                     /geo_loc_name="India"
                     /isolation_source="intertidal region"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. JJ66"
                     /strain="JJ66"
                     /submitter_seqid="scaffold56_size2199"
     gene            <1..>2199
                     /locus_tag="JJV70_RS22225"
                     /old_locus_tag="JJV70_22210"
     region          1..2199
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2199
                     /SMILES="NC(C(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2199
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:>2199](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>2199
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             1..>2199
                     /NRPS_PKS="Domain: AMP-binding (1-390). E-value: 1.2e-96.
                     Score: 315.7. Matches aSDomain:
                     nrpspksdomains_JJV70_RS22225_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (485-554). E-value: 6.6e-29. Score:
                     91.9. Matches aSDomain: nrpspksdomains_JJV70_RS22225_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (569-733). E-value:
                     5.1e-49. Score: 158.8. Matches aSDomain:
                     nrpspksdomains_JJV70_RS22225_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106261.1"
                     /locus_tag="JJV70_RS22225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_22210"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_219049828.1"
                     /sec_met_domain="AMP-binding (E-value: 4e-108, bitscore:
                     347.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.3e-18, bitscore:
                     53.6, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.6e-35, bitscore:
                     107.5, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVALVFGDEEVTYAELDARANWLAHHLIAAGVTRGDVVGVHIER
                     GPRMVVGLLAALKAGAAFTMLDVDYPAERLSGLLDRTDAAAVLHTAERAGHLTHPTARF
                     VDLDAEADEIAARPDHAPALPFHREDPAVVMFTSGSSGTPKGVIAPHRAIVRTLRAQDF
                     LAFGPDEVWLQSVPVSWDIFILELFGPLLTGARCVLQPGQKPDTVVLSELVYRHSVTST
                     WFSAGLFSVLVDEYPDVFAGSLRQVITGGEAPSISHLLRIVDSRPELRLVNGYGPAESM
                     VITNAHQIGAHDRGTPSVPIGVPLANTRVHVLDERLEPVPGGVVGELYVSGDGLAHGYV
                     GQPALTAERFVACPFGAPGEVMYRTGDLVRWRADGQIEYVGRGDFQVKIRGFRIEPGEV
                     EAAVAARPGVARSAVVVREDEPGEKRLVAYVVPRAGTSLDGRALREELGGPLPEYMVPS
                     AVVVLDXLPAPDFAALAGTGRAPRTPRETILCGLFAEVLGLRSVSVDDNFFELGGHSLL
                     ATRLTSRIRAGLGAEVGLRALFETPTVAGLAQRLGRAEAELRPALAPIEHAGEVLLSSA
                     QLRLWLIDRVQGPWSTYNAPILFGLSGSVDVVALDGALRDVTARHDTLRTVFPADAEDR
                     PRQEVRPVPGTVLRVVDVPAGADASARATEAARRPFDLAAELPFRAELFSVGGTEHVLA
                     LVSHHIVSDGWSFGVLARDLGVAYAARVSGRAPEWDALP"
     aSDomain        4..1170
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JJV70_RS22225_AMP-binding.1"
                     /evalue="1.20E-96"
                     /label="JJV70_RS22225_AMP-binding.1"
                     /locus_tag="JJV70_RS22225"
                     /protein_end="390"
                     /protein_start="1"
                     /score="315.7"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="AVALVFGDEEVTYAELDARANWLAHHLIAAGVTRGDVVGVHIERG
                     PRMVVGLLAALKAGAAFTMLDVDYPAERLSGLLDRTDAAAVLHTAERAGHLTHPTARFV
                     DLDAEADEIAARPDHAPALPFHREDPAVVMFTSGSSGTPKGVIAPHRAIVRTLRAQDFL
                     AFGPDEVWLQSVPVSWDIFILELFGPLLTGARCVLQPGQKPDTVVLSELVYRHSVTSTW
                     FSAGLFSVLVDEYPDVFAGSLRQVITGGEAPSISHLLRIVDSRPELRLVNGYGPAESMV
                     ITNAHQIGAHDRGTPSVPIGVPLANTRVHVLDERLEPVPGGVVGELYVSGDGLAHGYVG
                     QPALTAERFVACPFGAPGEVMYRTGDLVRWRADGQIEYVGRGDFQVKIR"
     aSModule        4..1662
                     /complete
                     /domains="nrpspksdomains_JJV70_RS22225_AMP-binding.1"
                     /domains="nrpspksdomains_JJV70_RS22225_PCP.1"
                     /locus_tags="JJV70_RS22225"
                     /monomer_pairings="Val -> Val"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       160..201
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JJV70_RS22225_0001"
                     /evalue="4.70E-03"
                     /label="NRPS-A_a2"
                     /locus_tag="JJV70_RS22225"
                     /protein_end="67"
                     /protein_start="53"
                     /score="10.6"
                     /tool="antismash"
                     /translation="LAALKAGAAFTMLD"
     CDS_motif       394..453
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JJV70_RS22225_0002"
                     /evalue="3.30E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="JJV70_RS22225"
                     /protein_end="151"
                     /protein_start="131"
                     /score="22.7"
                     /tool="antismash"
                     /translation="AVVMFTSGSSGTPKGVIAPH"
     CDS_motif       961..1083
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JJV70_RS22225_0003"
                     /evalue="1.10E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="JJV70_RS22225"
                     /protein_end="361"
                     /protein_start="320"
                     /score="49.7"
                     /tool="antismash"
                     /translation="PGGVVGELYVSGDGLAHGYVGQPALTAERFVACPFGAPGEV"
     CDS_motif       1135..1200
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JJV70_RS22225_0004"
                     /evalue="4.60E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="JJV70_RS22225"
                     /protein_end="400"
                     /protein_start="378"
                     /score="37.8"
                     /tool="antismash"
                     /translation="YVGRGDFQVKIRGFRIEPGEVE"
     aSDomain        1456..1662
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JJV70_RS22225_PCP.1"
                     /evalue="6.60E-29"
                     /label="JJV70_RS22225_PCP.1"
                     /locus_tag="JJV70_RS22225"
                     /protein_end="554"
                     /protein_start="485"
                     /score="91.9"
                     /tool="antismash"
                     /translation="ETILCGLFAEVLGLRSVSVDDNFFELGGHSLLATRLTSRIRAGLG
                     AEVGLRALFETPTVAGLAQRLGRA"
     aSDomain        1708..>2199
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JJV70_RS22225_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="5.10E-49"
                     /label="JJV70_RS22225_Condensation_LCL.1"
                     /locus_tag="JJV70_RS22225"
                     /protein_end="733"
                     /protein_start="569"
                     /score="158.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVSDG"
                     /tool="antismash"
                     /translation="EVLLSSAQLRLWLIDRVQGPWSTYNAPILFGLSGSVDVVALDGAL
                     RDVTARHDTLRTVFPADAEDRPRQEVRPVPGTVLRVVDVPAGADASARATEAARRPFDL
                     AAELPFRAELFSVGGTEHVLALVSHHIVSDGWSFGVLARDLGVAYAARVSGRAPEWDAL
                     P"
     CDS_motif       1774..1887
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JJV70_RS22225_0005"
                     /evalue="1.40E-12"
                     /label="C2_LCL_024-062"
                     /locus_tag="JJV70_RS22225"
                     /protein_end="629"
                     /protein_start="591"
                     /score="40.0"
                     /tool="antismash"
                     /translation="TYNAPILFGLSGSVDVVALDGALRDVTARHDTLRTVFP"
     CDS_motif       2092..2154
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JJV70_RS22225_0006"
                     /evalue="2.10E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="JJV70_RS22225"
                     /protein_end="718"
                     /protein_start="697"
                     /score="33.0"
                     /tool="antismash"
                     /translation="HHIVSDGWSFGVLARDLGVAY"
ORIGIN
        1 gacgcggtcg cgctggtctt cggcgacgag gaggtcacct acgccgagct cgacgcccgc
       61 gccaactggc tcgcccacca cctcatcgcc gccggcgtga cccgcgggga cgtcgtcggc
      121 gtccacatcg agcgcggccc ccggatggtc gtcggcctgc tggccgcgct caaggccggc
      181 gcggccttca ccatgctcga cgtggactac cccgccgagc ggctcagcgg cctcctcgac
      241 cggaccgacg ccgccgccgt cctccacacc gccgagcggg ccgggcacct cacccacccc
      301 accgcccggt tcgtggacct cgacgccgag gcggacgaga tcgccgcccg gcccgaccac
      361 gccccggccc tccccttcca ccgggaggac ccggcggtgg tcatgttcac ctccggctcc
      421 agcggcacgc ccaagggcgt gatcgccccg caccgggcga tcgtgcgcac cctgcgggcc
      481 caggacttcc tggccttcgg cccggacgag gtgtggctcc agtccgtccc cgtctcctgg
      541 gacatcttca tcctcgaact cttcgggccc ctgctcaccg gcgcccgctg cgtcctccag
      601 cccggccaga agcccgacac cgtcgtcctc tccgagctgg tctaccggca ctccgtgacc
      661 agcacctggt tctccgccgg cctcttctcc gtgctcgtgg acgagtaccc ggacgtcttc
      721 gctggcagcc tccgccaggt catcaccggc ggcgaggcac cgtccatctc gcacctgctg
      781 cggatcgtcg acagtcggcc ggagctgcgc ctcgtcaacg gctacgggcc ggccgagagc
      841 atggtcatca ccaacgccca ccagatcggc gcccacgacc ggggcacccc ctcggtgccc
      901 atcggtgtcc ccctggccaa cacccgcgtc cacgtcctcg acgaacggct ggagcccgtc
      961 cccgggggtg tcgtcggcga gctgtacgtc tccggcgacg gcctcgccca cggctacgtc
     1021 ggacagcccg cgctgaccgc cgagcggttc gtggcctgcc cgttcggcgc ccccggcgag
     1081 gtgatgtacc gcaccggcga cctggtgcgg tggcgtgccg acggccagat cgagtacgtc
     1141 gggcgcggcg acttccaggt gaagatccgc ggcttccgga tcgagcccgg cgaggtcgag
     1201 gcggccgtgg ccgcccggcc cggcgtggcc cgttcggcgg tcgtcgtccg cgaggacgag
     1261 ccgggggaga agcggctggt cgcctacgtc gtgccccggg ccgggacgag cctcgacggg
     1321 cgcgcgctgc gggaggagtt gggcgggccg ctgcccgagt acatggtccc ctccgccgtg
     1381 gtcgtcctcg acnccctgcc cgcccccgac ttcgccgccc tcgccggcac cggccgggcc
     1441 ccgcgcaccc cccgggagac catcctgtgc gggctgttcg ccgaggtcct ggggctgcgc
     1501 tcggtctcgg tcgacgacaa cttcttcgag ctgggcggcc actcgctgct cgccacccgc
     1561 ctgaccagcc ggattcgcgc cgggctcggc gccgaggtcg gcctgcgggc cctcttcgag
     1621 acccccaccg tcgccggtct cgcccagcgg ctcgggcggg ccgaggcgga gctccggccc
     1681 gcgctcgccc cgattgagca cgccggggag gtgctgctgt cctccgcgca gctgcggctg
     1741 tggctgatcg accgggttca gggcccgtgg tccacctaca acgcgccgat actcttcggc
     1801 ctgagcgggt ccgtcgacgt ggtggcgctc gacggcgccc tgcgcgatgt cacggcccgg
     1861 cacgacacgc tgcggaccgt cttccccgcc gacgccgagg accggccccg gcaggaggtg
     1921 cggcccgttc ccgggaccgt gctgcgcgtg gtggacgtgc cggccggcgc ggacgcgtcc
     1981 gcacgggcga cggaggccgc ccgtcggccc ttcgacctgg cggccgaact gcccttccgg
     2041 gccgagctgt tctcggtggg cggcacggag cacgtgctcg ccctggtctc gcaccacatc
     2101 gtcagcgacg gctggtcctt cggcgtcctc gcccgtgacc tcggggtggc gtacgcggcc
     2161 cgggtgtcgg gtcgggcgcc ggagtgggac gccctgccg
//
