LOCUS       NZ_JAEVFI010000053      5650 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Streptomyces sp. JJ66 scaffold53_size5650, whole genome shotgun
            sequence.
ACCESSION   NZ_JAEVFI010000053
VERSION     NZ_JAEVFI010000053.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. JJ66
  ORGANISM  Streptomyces sp. JJ66
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Jose,P.A. and Jha,B.
  TITLE     Draft genome of Streptomyces sp. JJ66
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Jose,P.A. and Jha,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2021) Marine Biotechnology and Ecology Division,
            CSIR - Central Salt and Marine Chemicals Research Institute, G.B.
            Marg, Bhavnagar, Gujarat 364002, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-MAY-2017
            Assembly Method                   :: Velvet v. V 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 320.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019303495.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 03:51:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,446
            Genes (coding)                    :: 4,340
            CDSs (with protein)               :: 4,340
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 61
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 31 of 106
            Pseudo Genes (incomplete)         :: 82 of 106
            Pseudo Genes (internal stop)      :: 3 of 106
            Pseudo Genes (multiple problems)  :: 9 of 106
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:22:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5650
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAEVFI010000053.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5650
                     /collected_by="Polpass Arul Jose"
                     /collection_date="2015-10-14"
                     /db_xref="taxon:2803843"
                     /geo_loc_name="India"
                     /isolation_source="intertidal region"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. JJ66"
                     /strain="JJ66"
                     /submitter_seqid="scaffold53_size5650"
     region          1..5650
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..5650
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5650
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2375:4957](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2376..4957
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            complement(195..1112)
                     /locus_tag="JJV70_RS22180"
                     /old_locus_tag="JJV70_22165"
     CDS             complement(195..1112)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1226: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019984977.1"
                     /locus_tag="JJV70_RS22180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_22165"
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="WP_219049821.1"
                     /transl_table=11
                     /translation="MPGVPRWTGREAALLRKAMRKSGKAFADLVGVNPRQIPRWEGRGE
                     TLELSLANQAALDTVLAQVDRDAQQRFATLLTERAAVEHDERAEALLRRDSRTSQHPAD
                     GKLMVCVEEGIYLSGLEDRSVWLGTYRIDVYPTTNADYAKFVHATGHRPPLHWADGRYP
                     AGLATHPVVWVTWHDADAYARWAGKTLPTAPQWEKAARGAKGRVYPWGNDPTAAKCNVA
                     ETGIDATTSVSRYQSGVSPYGVFDLCGNVWEWCSTEEQSGSARYELKGSAFTSPFERAA
                     PSLKNTADARMQDNDTGFRCVSIP"
     misc_feature    complement(1068..1070)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(1122..1904)
                     /locus_tag="JJV70_RS22185"
                     /old_locus_tag="JJV70_22170"
     CDS             complement(1122..1904)
                     /NRPS_PKS="Domain: Aminotran_4 (39-252). E-value: 2.7e-25.
                     Score: 81.4. Matches aSDomain:
                     nrpspksdomains_JJV70_RS22185_Aminotran_4.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019766742.1"
                     /locus_tag="JJV70_RS22185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_22170"
                     /product="aminotransferase class IV family protein"
                     /protein_id="WP_219049822.1"
                     /transl_table=11
                     /translation="MATLNGKPVSADDLLPLALTNLGHFTSMRVDETGGIRGATLHLER
                     LVRDCKAIWGADLDTGRVRDFIRQSVEGYDRPCVARVTVYDPKVDMGYPAAADEPHILV
                     SVREAGGMPPAPLRAMSVPYERDLPEVKHVGLLGALHARRTAQLAGYGDALFVGRDGLV
                     SEGGTWNVGFVDQGGTIVWPQAPVLPGVTMALLQQHTEHRVAPVTLGQAKSMAAAFATN
                     TSIGVRALSTIDEAPFPTEHAAVRRLQEAYLSIPAETL"
     aSDomain        complement(1149..1787)
                     /aSDomain="Aminotran_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JJV70_RS22185_Aminotran_4.1"
                     /evalue="2.70E-25"
                     /label="JJV70_RS22185_Aminotran_4.1"
                     /locus_tag="JJV70_RS22185"
                     /protein_end="252"
                     /protein_start="39"
                     /score="81.4"
                     /tool="antismash"
                     /translation="TLHLERLVRDCKAIWGADLDTGRVRDFIRQSVEGYDRPCVARVTV
                     YDPKVDMGYPAAADEPHILVSVREAGGMPPAPLRAMSVPYERDLPEVKHVGLLGALHAR
                     RTAQLAGYGDALFVGRDGLVSEGGTWNVGFVDQGGTIVWPQAPVLPGVTMALLQQHTEH
                     RVAPVTLGQAKSMAAAFATNTSIGVRALSTIDEAPFPTEHAAVRRLQEAY"
     gene            2153..2293
                     /locus_tag="JJV70_RS22190"
                     /old_locus_tag="JJV70_22175"
     CDS             2153..2293
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF033525.2"
                     /locus_tag="JJV70_RS22190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_22175"
                     /product="albusnodin family lasso peptide"
                     /protein_id="WP_219049823.1"
                     /transl_table=11
                     /translation="METENDQPDDGPVADAPVLLVSMGEASGVTLGQGMGSSEDKRRAY
                     N"
     gene            2376..4199
                     /locus_tag="JJV70_RS22195"
                     /old_locus_tag="JJV70_22180"
     CDS             2376..4199
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF033561.1"
                     /locus_tag="JJV70_RS22195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_22180"
                     /product="albusnodin/ikarugamycin family macrolactam
                     cyclase"
                     /protein_id="WP_219049824.1"
                     /sec_met_domain="Asn_synthase (E-value: 1.4e-29, bitscore:
                     92.5, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRWFGGFCNPTAPPRAPADSARPWPTTPGLWTVGQWAGEEVRTMC
                     TGRRFVAVIGPSGIARRDLARLGTFGVPDDVAWRWPGSYTTVEVTDEFTRVWTDLGGAW
                     PVYTTAADGGVYWASSSRALAGLTHTGPNVDRLAAWLLAPSVPVLLAGRSAFAGVELVP
                     AGHRLTVARDGVVRTNQMWSPRPRPGAADHRLRAELAAAVAVRVVAASAPTVDLSGGYD
                     STALALLAHESVGPDHAVTGVTVCPVGHPSGGDLDYARLVAERSGIAHRLMPLDADHAP
                     YTSLCAVPVTDEPAPSTVAHARFSGQLHWMRTTLGTDCHMTGDGGDSLLCSPPIMLADL
                     LATGRNRRGVVETIRWARLRRLPVWSLLVSAYRTSRSNRADALARLSVELFTGVRKAVP
                     KGDIGWCATDLVPSWATYDARARAAAVASSVPQRFPEAAIGMFAATVAAEGIADVGRSA
                     RADIQLAETVGVPLHNPFTDSRVVDAYLSIPLDERPGSADYKPVLRDALADLFPAELAA
                     RTTKGDFNPDHYHGMRTNLPELHALADGQLAALGLVDPVELRRTLAMTAAGLPVPFSTV
                     EPAVAAEVWLRALADAPDVVWTPVAAGEEAK"
     misc_feature    3999..4001
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    4131..4133
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            4196..4957
                     /locus_tag="JJV70_RS23430"
                     /old_locus_tag="JJV70_22185"
     CDS             4196..4957
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086158.1"
                     /locus_tag="JJV70_RS23430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_22185"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_219049825.1"
                     /sec_met_domain="PF13471 (E-value: 1.1e-20, bitscore: 62.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRPRYITVPEHVRAINVGPATVIVNYRNGRAETLIGPAARWWDE
                     ATTSGYAEVPGVLDGLAEQTLCRQLLEAGLISPTFSRRPGQHTASGPPWVPSWGTQEMT
                     AGRGEPVRVPFGVTARAGVALAVVLGVLGAGRKRRRMARLVWLLTWIARRSTGSATADQ
                     ARLAINAVRRAGLVVPGRVACLEESAAVAVMLALSRQRVTWCHGAAADPVRLHAWVETD
                     DGHRVAEPPSTARFAVLRAIPERDNGGENDA"
     gene            4950..>5200
                     /locus_tag="JJV70_RS23075"
                     /pseudo=""
     CDS             4950..>5200
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086157.1"
                     /locus_tag="JJV70_RS23075"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MHEHGEPIVWIHGDRAGLGPLSGDLVDLYWQWEQDPGVLIGYGRQ
                     TPDSLENRNEGFQHQARSTDNQLRFTVYDMTTTPLTPV"
     assembly_gap    5201..5215
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
ORIGIN
        1 ctcgcgcgag tagcccgcga tctcgaacgc ctcgtacatg atctccggct cgtggttccg
       61 gatggcatcg gaggcccgct cgctagcgcc gcacagctgt ccagacggga ggcaggcgcg
      121 cgtgaggaaa gcggcgtgcg cagtactggc gcagcacccg ccgacaaccg gactcggcgc
      181 gtgccggact gggcttacgg aatgctcacg caacggaatc cggtgtcgtt gtcctgcatg
      241 cgggcgtccg ccgtgttctt cagcgacggc gcggcacgct cgaacggcga ggtgaaggcc
      301 gaacccttca gctcgtaccg ggcgctgccg gattgctcct cggtggaaca ccactcccat
      361 acgttgccac agaggtcgaa gacgccgtag gggctgacgc cggactggta gcgggagaca
      421 gacgtcgtgg cgtctatgcc ggtttcggcg acgttgcact tcgcggctgt ggggtcgttg
      481 ccccacgggt agacgcggcc cttcgcgccg cgcgcggcct tctcccactg tggggccgtg
      541 ggcaatgtct taccggccca acgggcgtac gcgtcggcgt cgtgccacgt cacccacacc
      601 actggatgcg tcgccagacc ggccgggtac cggccgtctg cccagtgcag gggcggccgg
      661 tgaccggtgg cgtgtacgaa cttcgcgtag tcggcgttgg tcgtcgggta tacgtcgatg
      721 cggtatgtcc ccagccacac cgagcgatct tccaggccgg agaggtagat accctcctcg
      781 acgcagacca tcagctttcc gtcggcgggg tgctgactgg ttcgcgagtc gcggcgcagc
      841 agtgcctcgg cgcgctcgtc gtgctcgact gcggcccgct ccgtgaggag cgtcgcgaat
      901 cgctgctggg cgtcgcggtc aacttgcgcg agcacggtgt caagagccgc ttgattggcg
      961 aggctcagtt ccagagtctc gccccgacct tcccagcggg ggatctggcg cggattcacc
     1021 cccaccagat cggcgaatgc cttgccgctc ttgcgcatgg ccttgcgtaa cagggcggct
     1081 tcgcggcctg tccatctggg cacccccggc atgctgtcgt gctacagggt ctcggcgggg
     1141 atcgacaggt acgcctcttg aagtcgtctc acagcggcgt gctcggtggg gaagggggcc
     1201 tcgtcgatgg tggacagggc gcggacgccg atgctcgtgt tcgtcgcgaa cgccgccgcc
     1261 atgctcttgg cctgtcccag ggtgaccggg gccacccggt gctcggtgtg ctgttggagc
     1321 agtgccatgg tcacgccggg aagcaccggg gcctgcggcc acacgatggt tccgccctgg
     1381 tcgacgaagc cgacgttcca ggtgccgcct tcggaaacca atccgtcgcg gccgacgaac
     1441 agggcgtcgc cgtatccggc gagctgcgcg gtgcggcgcg cgtgcagtgc tccgagcagc
     1501 ccgacgtgct tgacctcagg gaggtctcgt tcgtagggaa cgctcattgc gcgcagcggc
     1561 gccgggggca ttccgccagc ttcgcgcaca gaaaccagga tgtgtggctc gtcggcggct
     1621 gccgggtatc ccatatcgac cttcgggtca tagaccgtga cgcgggccac gcacggccgg
     1681 tcgtacccct ccacactctg gcgaatgaag tcccggacgc gtccggtgtc caggtctgcg
     1741 ccccagatcg ccttgcagtc ccgcacgaga cgttccaggt gcagcgtcgc accgcggatg
     1801 ccgccggtct catccacccg catcgaggtg aagtgaccga ggttggtcag ggccaggggc
     1861 aacaggtcgt cggcggatac gggctttcca ttgagcgtcg ccatgccgcc cagcatgtca
     1921 gggtggccgc tcctgcggca ccccaagcgg agttactgac ggggcaacgt catccgcacg
     1981 tcattcccat gccgccctca cagggtgttt gctggtcacc ggccggacac gtttggtgac
     2041 tacgtccccg gacggcccgc aagacacccg acgcggctgt cccgtcgcgg ccgggtgtgc
     2101 aaggagcgaa cgtgcggcac aagccgcgtg gataccgcga aaggaggcag aaatggagac
     2161 ggagaacgac cagccggatg acggcccggt ggccgacgcc cccgtgctgc tggtcagcat
     2221 gggggaggcg tcgggggtga cgctcggcca gggcatgggc agctccgagg acaagcggcg
     2281 ggcctacaac tgacccgcct ccgtgctggt gcccggaccg gatgcgggct gggcgccagc
     2341 cacgttcagg tcaacaccgc aggaaggctc agcgtatgcg ttggttcggg ggtttctgca
     2401 accccacagc accaccaaga gccccggccg acagcgcccg accgtggcct actacgccgg
     2461 ggctctggac ggtcggccag tgggccggag aagaggtccg cacgatgtgc accggccgcc
     2521 ggttcgtggc cgtgatcggc ccgagcggca ttgcacggag agacctcgcc cggcttggca
     2581 ctttcggcgt tcccgatgac gtggcgtggc gctggccggg gagctacacg acagtagaag
     2641 tcactgatga gtttacgcga gtgtggaccg acctcggcgg agcatggccg gtctacacca
     2701 cggcggcaga cggcggcgtg tactgggcgt cgagctcccg cgccctggcc gggctgaccc
     2761 acaccggtcc gaacgtggac cgacttgccg catggttgct tgccccttcc gtgcccgtac
     2821 tgctggctgg acggtccgcg ttcgcgggcg tggaactcgt gccagccggg catcgcctga
     2881 ccgtggcacg ggacggagtg gttcggacga accagatgtg gtcgcctcga ccgcgcccag
     2941 gggccgccga tcaccggttg cgggctgagc tcgcggcggc tgtggccgta agagtggtcg
     3001 cagcgtccgc gccgaccgtg gacctgtcgg gcggatacga ctccaccgcg ctggcactgc
     3061 tagcgcacga atcggttggt cccgatcatg cggtgaccgg ggtgactgtc tgcccggtcg
     3121 gccacccatc gggcggagac ctggactacg cgcggttggt cgccgagcgg tccgggatcg
     3181 cgcaccggct catgccgttg gacgccgacc acgcgcccta cacctcgttg tgcgcggtcc
     3241 ctgtcaccga tgagcccgcg ccatcaacgg tcgcgcacgc gcgattttcc ggtcaactcc
     3301 actggatgcg caccacactt ggaaccgatt gtcacatgac cggcgacggt ggagacagcc
     3361 tgttgtgctc ccctccgatc atgctcgccg acctgttggc taccgggcgc aaccggcggg
     3421 gcgtggtcga aacgatccgc tgggcaaggc tgcgtcggct tccagtgtgg tcgctgctgg
     3481 tgtcggccta ccgcacctca cgctcgaatc gtgcggatgc gctggcacgc ttgtctgtgg
     3541 agctcttcac aggcgttcga aaggccgttc cgaagggaga catcggttgg tgtgcaacgg
     3601 acttggtgcc gtcgtgggct acctacgacg cgcgggcacg ggctgcggcc gtcgcgtcat
     3661 cggtgcctca acggtttccc gaggccgcca tcggtatgtt cgccgcgacc gtggcagccg
     3721 aagggatagc cgacgtgggc cgctcggcac gcgccgacat tcagctggcc gaaacggtcg
     3781 gggtgccgct gcacaacccg ttcaccgact cccgcgtggt ggacgcatac ctgtcgattc
     3841 ccctggacga gcgacccggc tccgctgact acaaaccggt cctgcgggat gccctggccg
     3901 acctgttccc cgctgaactg gccgcgcgga ccaccaaagg tgacttcaac cccgaccact
     3961 accacgggat gcgtacgaac ctgccggagc ttcacgcatt agcggacggc cagttggctg
     4021 cgctgggcct cgtggacccc gtagagctcc ggcgcacgct ggcgatgacg gcggccggac
     4081 tgcctgtccc cttctccacg gtggaacctg cggtggcagc ggaggtgtgg ttacgtgctt
     4141 tggccgacgc cccggacgtg gtgtggacac cggtagcggc cggggaggag gccaagtgaa
     4201 gcggccccgg tacatcaccg ttcccgagca cgtccgcgct atcaacgtcg gcccggcaac
     4261 ggtgatcgtg aactaccgca acggccgcgc agagacgctg atcggcccgg ccgcgcgctg
     4321 gtgggacgag gcgacaacca gcggctacgc cgaggtgccc ggcgtgctgg acggactggc
     4381 cgaacagacg ttgtgtcgcc agcttctgga ggctggactg atctcgccga ctttctcccg
     4441 ccgaccgggg cagcacacag cctctgggcc gccgtgggtg cccagttggg gcacacagga
     4501 gatgacggcc gggcgtggcg agccggtccg cgtgccgttc ggagtgacag cgcgcgcggg
     4561 cgtggccttg gctgtcgtgc tcggcgtgct gggggcaggc cgcaagcgcc gtcgcatggc
     4621 acggctcgtg tggctgctga catggatagc ccggcgtagc accggcagcg ccacagccga
     4681 tcaggctcgc ctagccatca acgcggtacg acgggctggg ctggtcgtac cgggccgcgt
     4741 ggcctgcctg gaagaatcag cggccgtcgc tgtgatgctg gccctatccc gccaacgggt
     4801 gacgtggtgc cacggtgcgg cggccgaccc cgtccggctg cacgcatggg tggaaactga
     4861 tgacggacat cgggtggccg agccgccatc tacggcgcgg ttcgccgtgc tgcgcgccat
     4921 cccggaacgt gacaacggag gcgagaacga tgcatgagca tggcgaacca atcgtgtgga
     4981 ttcacgggga ccgtgccgga ctcggcccgc tctccggtga cctggtggac ctgtactggc
     5041 agtgggaaca agaccccggc gtgctgatcg gctacggccg tcagacaccg gactcactgg
     5101 agaaccgcaa cgaaggattc cagcaccagg cgcgcagcac cgacaaccaa ctacgcttca
     5161 ccgtctacga catgaccacg acgcctctga cgcccgtcgg nnnnnnnnnn nnnnnaaaag
     5221 gccgggttcc gcaagatcgg tgagcgtcgg cagtccggct actggctcgg gcagtccgcc
     5281 aacgaaacac tcatggacgc cgttcccgcc gacttccccg gcccgtccgt tgtgcgacac
     5341 atcgttgaac cctcccggta ggcacccata ccggccccag cgccggggtg gtcggtgcga
     5401 gcccgcctcg ccgctgtcag tgttgccagg ccacgttcag tgactgcaca gggggccgta
     5461 tgggaggcat ggggtgagga gggggacccg ctatgccgcc ctccttaccc cgacgtgctg
     5521 cgggtgcggc tgccaggggc gcagaccccc cacgtggcct gaggccccac ggcgcccgcg
     5581 catggctggt gcggcacgcg gtacgcgggc gtccgtcggg tggggtgcta cttcggcggg
     5641 ggcttgcgca
//
